CN112126596B - Streptomyces spectabilis with disease-resistant and growth-promoting effects and separation method and application thereof - Google Patents
Streptomyces spectabilis with disease-resistant and growth-promoting effects and separation method and application thereof Download PDFInfo
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Abstract
The invention discloses a streptomyces spectabilis strain with disease-resistant and growth-promoting effects, a separation method and application thereof, and belongs to the technical field of biological control. The invention discloses a strain of streptomyces spectabilis named as streptomyces spectabilis ()Streptomyces spectabilis)69-1, Streptomyces spectaculeatus 69-1 is now deposited in a depository designated by the national intellectual Property office: china center for type culture Collection, with a preservation date of 2019, 11 months and 25 daysThe serial numbers are: CCTCC NO: m2019974; the strain can prevent and control plant fungal diseases such as tobacco black shank, can also prevent and control plant bacterial diseases such as bacterial wilt, explores a new source of biological pesticides, and the streptomyces spectabilis 69-1 can also promote the growth and development of tobacco plants.
Description
Technical Field
The invention relates to the technical field of biological control, in particular to a streptomyces spectabilis strain with disease-resistant and growth-promoting effects, and a separation method and application thereof.
Background
During the growth process of crops, various soil-borne diseases can occur, particularly in continuous cropping soil, and various diseases are high. Among these, fungal and bacterial diseases are common forms of plant diseases. The method for preventing and treating the plant diseases mainly comprises soil fumigation, chemical sterilization pesticides or microbial agents with disease resistance. The fumigation has been banned by the state due to the huge destructive effect on the environment. Chemical pesticides are undesirable to consumers because they can remain in the soil and in crops, causing various hazards to humans. Promoting the growth of crops mainly by using fertilizers and growth regulators. Wherein, the fertilizer is mainly a chemical fertilizer. The national ministry of agriculture proposes ' zero increase action plan of fertilizer usage to 2020 and ' zero increase action plan of pesticide usage to 2020 ', and defines strict agricultural control of fertilizer and pesticide. The microorganism has the advantages of pure nature, no residue and the like, becomes an important material for preventing and treating plant diseases and promoting plant growth, and is a development direction encouraged by the nation.
Ralstonia solanacearum is a gram-negative soil-borne plant pathogenic bacterium, and is one of ten kinds of plant pathogenic bacteria (Ralstonia solanacearum) which are widely distributed and seriously damaged in the world (Ralstonia solanacearum ranks the second name). The ralstonia solanacearum has a wide host range, and can infect more than 450 plants of 54 families, including dicotyledonous herbaceous plants (such as solanaceae, leguminosae and the like), dicotyledonous woody plants (such as mulberry, eucalyptus, casuarina equisetifolia and the like) and monocotyledonous plants (such as banana, ginger and the like). Ralstonia solanacearum can cause destructive wilt (also called bacterial wilt) of various important economic crops (such as tobacco, tomatoes, potatoes, ginger and the like), causes the loss of diseased land of up to 95 percent, and is an important limiting factor in the production of many crops and economic crops.
Phytophthora parasitica (Phytophthora parasitica) is one of the most devastating fungal diseases, the tobacco blight is also called blackleg, and the tobacco growers are called blackleg crazy, blackroot and aconite. All main smoke producing areas occur in different degrees, wherein Anhui, Shandong and Henan provinces are historically serious disease areas; the occurrence of tobacco in southern areas such as Yunnan, Guizhou, Sichuan, Hunan, Guangdong, Guangxi and Fujian is also quite common. Foot rot of citrus, epidemic rot of peach, and neck rot of peach are all caused by phytophthora parasitica. After phytophthora parasitica causes plant diseases, other diseases are associated, for example, tobacco black shank is mixed with bacterial wilt, and citrus foot rot is associated with fusarium, so that the damage is more serious. However, most of the common microbial agents have single resistance, namely antifungal or antibacterial, and a broad-spectrum biological control bacterium which can resist both fungi and bacteria is lacked.
Disclosure of Invention
In view of the research background, the invention aims at providing a streptomyces spectabilis strain with disease-resistant and growth-promoting effects.
The application provides a strain of Streptomyces spectabilis, named Streptomyces spectabilis (Streptomyces spectabilis)69-1, and the Streptomyces spectabilis 69-1 is deposited in a deposit unit specified by the national intellectual property office: the preservation date of the China center for type culture Collection is 2019, 11 and 25 months, and the preservation numbers are: CCTCC NO: m2019974, deposit unit address: wuhan Lojia mountain, Wuhan university, postal code 430072.
The invention also aims to provide a method for separating and obtaining the Streptomyces spectabilis 69-1, which is obtained by separating and obtaining the Streptomyces spectabilis 69-1 from flower garden humus soil in university campus of Yunnan Queenming and adopting a plate dilution method.
The process for separating and obtaining the streptomyces spectabilis 69-1 comprises the following steps: preparing a potato glucose agar culture medium (PDA), inoculating Phytophthora nicotianae (Phytophthora parasitica var nicotianae) on the PDA culture medium, after the Phytophthora is overgrown on a flat plate, spreading a soil sample collected from humus in a flower garden in campus of university of Yunnan on the flat plate overgrown with the Phytophthora, culturing for one week at 25 ℃, forming a colony of a transparent ring on the Phytophthora flat plate, wherein the colony is a strain with the function of resisting Phytophthora fungi, then picking out the strain, and storing the strain in a PDA inclined plane. Meanwhile, a beef extract peptone agar plate is prepared, Ralstonia solanacearum (Ralstonia solanacearum) is coated and inoculated, a Ralstonia solanacearum plate is prepared, a phytophthora resistant strain obtained from the phytophthora plate is inoculated on the Ralstonia solanacearum plate, and a bacterium capable of forming a transparent ring is a streptomyces spectabilis 69-1 strain capable of resisting both fungi and bacteria.
The spectinomycin 69-1 is on the PDA culture medium, the colony is limited, the edge is provided with wrinkles, and the colony is red. The spore has more than 10-50 spores, and has straight and curved spore silk and pseudorecurrent spore. The spores are oval and have smooth surfaces, as shown in FIG. 1.
Results for homology sequence of 16 SrDNA: as shown in FIG. 2, Streptomyces spectaculeatus 69-1 and Streptomyces spectaculeatus are gathered into one branch, and the morphological characteristics are combined, which indicates that the streptomyces spectaculeatus belongs to.
In order to better implement the invention, the invention further aims at the application of the streptomyces spectabilis 69-1 in preventing and treating tobacco black shank and bacterial wilt.
In order to better implement the invention, a fourth object of the invention is the use of Streptomyces spectabilis 69-1 for promoting the growth and development of tobacco.
The application comprises the steps of preventing and treating tobacco black shank and bacterial wilt by using Streptomyces spectaculeatus 69-1 fermentation liquor and promoting tobacco growth and development, wherein the Streptomyces spectaculeatus 69-1 fermentation liquor is obtained by inoculating a spore suspension of Streptomyces spectaculeatus 69-1 to a potato-oat meal agar culture medium for culture.
The spore concentration in the Streptomyces spectabilis 69-1 fermentation liquor is 1 multiplied by 107-1×1012cfu/ml。
The fermentation liquor of the streptomyces spectabilis 69-1 is prepared by the following method: inoculating the seed solution of the streptomyces spectabilis 69-1 to a potato-oat powder liquid culture medium, and culturing at the temperature of 28 ℃ for 80-180 r.min-1Culturing for 6-10 days in a shaking way to obtain the Streptomyces spectabilis 69-1 fermentation liquor, wherein the inoculation amount of the seed liquor is 1-10% of the volume of the potato-oat flour liquid culture medium;
the seed solution is prepared by the following method: growing the spectinomycin on the slant of the potato-oat flour liquid culture medium for 4-8 days to obtain a seed liquid.
The application method of the Streptomyces spectabilis 69-1 fermentation liquor comprises the following steps:
A. when crops are cultivated, digging cultivation holes corresponding to the sizes of seedling roots in soil, placing seedlings, and pouring the Streptomyces spectaculeatus 69-1 fermentation liquid at the roots of the plants, wherein the using amount of the Streptomyces spectaculeatus 69-1 fermentation liquid is 10ml-100ml per plant according to the sizes of the roots; or the like, or, alternatively,
B. mixing 10ml-100ml of fermentation liquor of Streptomyces spectabilis 69-1 with water for root watering in any proportion, and watering the mixture into a cultivation hole when crops are cultivated, or watering the mixture after soil covering; or the like, or, alternatively,
C. adding the fermentation liquor of the streptomyces spectabilis 69-1 into the sterilized wheat bran, drying at low temperature to prepare fungus powder, and applying 0.1-10 g of the dried fungus powder to a field when plants are cultivated; or the like, or, alternatively,
D. adding Streptomyces spectabilis 69-1 fermentation liquor into sterilized wheat bran, drying at low temperature to obtain fungus powder, activating the fungus powder 0.1-10 g/strain in nutrient solution, and pouring into plant root during cultivation; or the like, or, alternatively,
E. freeze-drying the fermentation liquor of the spectinomycin 69-1 to prepare 0.01-1 g of pure bacterial powder per plant, and directly applying the pure bacterial powder when cultivating plants; or the like, or, alternatively,
F. freeze-drying Streptomyces spectabilis 69-1 fermentation liquor to prepare pure bacterial powder, and mixing 0.01-1 g/strain of the pure bacterial powder with an organic fertilizer for application; or the like, or, alternatively,
G. freeze drying Streptomyces spectabilis 69-1 fermentation liquor to prepare pure bacterial powder, activating bacterial powder by 0.01-1 g/strain, and pouring bacterial liquid containing activated bacteria on the roots of plants.
Compared with the prior art, the invention has the following advantages and effects:
the invention aims to solve the problems that the chemical method adopted in the prior art for preventing and treating the tobacco black shank and the bacterial wilt has large limitation, is difficult to eradicate and has serious environmental pollution caused by chemical pesticides and the problem that the biological bactericide is difficult to simultaneously prevent and treat fungal diseases and bacterial diseases, explores a new source of the biological pesticides, separates the biological bactericide from soil to obtain the spectinomycin 69-1 which can be used for preventing and treating the plant fungal diseases such as the tobacco black shank and the bacterial wilt, has high efficiency and can be industrially produced, and the spectinomycin 69-1 can promote the growth and development of tobacco plants.
The invention applies the streptomyces spectabilis 69-1 to the control of the tobacco black shank and bacterial wilt and the promotion of the growth and development of tobacco plants, particularly adopts the fermentation liquor of the streptomyces spectabilis 69-1 to control the tobacco black shank and the bacterial wilt and promote the growth and development of the tobacco plants, and has simple preparation method, high control efficiency and simple use method.
Drawings
FIG. 1 is a diagram showing the growth state of Streptomyces spectabilis 69-1 on PDA plates.
FIG. 2 is a phylogenetic tree of a strain similar to Streptomyces spectabilis 69-1 constructed based on the 16SrDNA gene sequence.
FIG. 3 shows the disease prevention effect of Streptomyces spectaculeatus 69-1 in continuous cropping soil, wherein the graph shows the effect of a control group without applying a microbial inoculum in the left behavior and the effect of Streptomyces spectaculeatus 69-1 in the right behavior, and the graph shows that the strain can not only prevent and treat soil-borne diseases, but also promote plant growth.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding of the skilled person.
The formula of the culture medium in the following examples of the invention is as follows:
the potato-oat flour agar culture medium comprises the following components in percentage by weight: peeling potato 200g, boiling for half an hour, filtering, adding glucose 10g, oat flour 30 g, adding 1000 mL tap water, and sterilizing with high pressure steam at 121 deg.C for 20 min.
The formula of a Potato Dextrose Agar (PDA) culture medium is as follows: peeled potato 200g, glucose 20g, distilled water 1000 mL, natural pH. Boiling rhizoma Solani Tuber osi for half an hour, filtering, adding 1000 mL tap water, and sterilizing with high pressure steam at 121 deg.C for 20 min.
Example 1 isolation and characterization of Streptomyces spectabilis 69-1
The application provides a strain of Streptomyces spectabilis, named Streptomyces spectabilis 69-1(Streptomyces spectabilis 69-1), and the Streptomyces spectabilis 69-1 is deposited in a deposit unit specified by the national intellectual property office: the preservation date of the China center for type culture Collection is 2019, 11 and 25 months, and the preservation numbers are: CCTCC NO: m2019974, deposit unit address: wuhan Lojia mountain, Wuhan university, postal code 430072.
The process for separating and obtaining the streptomyces spectabilis 69-1 comprises the following steps: preparing a potato glucose agar (PDA) culture medium, inoculating Phytophthora nicotianae (Phytophthora paragentica var nicotianae) on the PDA culture medium, after the Phytophthora is overgrown on a plate, scattering a soil sample obtained from humus soil in a flower garden of university campus of Yunnan on the plate overgrown with the Phytophthora, culturing for one week at 25 ℃, forming a colony of a transparent ring on the Phytophthora plate, wherein the colony is a strain with the function of resisting Phytophthora fungi, then selecting the strain, and storing the strain in a PDA inclined plane. Meanwhile, a beef extract peptone agar plate is prepared, Ralstonia solanacearum (Ralstonia solanacearum) is coated and inoculated, a Ralstonia solanacearum plate is prepared, the phytophthora resistant strain obtained from the phytophthora plate is inoculated on the Ralstonia solanacearum plate, and the bacterium capable of forming a transparent ring is the streptomyces spectabilis 69-1 strain capable of resisting both fungi and bacteria.
The Streptomyces spectabilis 69-1 is on the PDA culture medium, the colony is limited, the edge is provided with wrinkles, and the colony is red. The spore has more than 10-50 spores, and has straight and curved spore silk and pseudorecurrent spore. The spores are oval and have smooth surfaces, as shown in FIG. 1.
16SrDNA homology sequence results: as shown in FIG. 2, Streptomyces spectinolyticus 69-1 and Streptomyces spectinolyticus are gathered into one branch, and the morphological characteristics are combined, which indicates that the Streptomyces spectinolyticus belongs to the Streptomyces spectinolyticus.
Example 2 preparation of Streptomyces spectabilis 69-1 fermentation broth
Inoculating a spore suspension of the spectinostreptomyces 69-1 to a potato-oat flour agar culture medium, and culturing at 28 ℃ for 80-180 r.min-1Culturing for 6-10 days in a shaking way to obtain the Streptomyces spectabilis 69-1 fermentation liquor, wherein the inoculation amount of the seed liquor is 1-10% of the volume of the potato-oat flour liquid culture medium;
growing Streptomyces spectabilis 69-1 on potato-oat flour liquid culture medium for 4-8 days, and making into seed liquid.
Example 3 Effect of different microbial inoculum treatment on agronomic traits of tobacco plants
Selecting 90 tobacco seedlings with the same growth condition, and averagely dividing the tobacco seedlings into three groups, wherein the first group is a blank control group without applied microbial inoculum, the second group is bacillus subtilis wettable powder, and the third group is streptomyces spectabilis 69-1 fermentation liquor; when crops are cultivated, cultivation holes corresponding to the sizes of seedling roots are dug in soil, and seedlings are placed in the cultivation holes. The first group does not contain any microbial inoculum, the second group pours bacillus subtilis wettable powder after dissolving in water on the roots of plants, and the third group pours streptomyces spectabilis 69-1 fermentation liquor on the roots of plants, and the dosage is 60ml per plant according to the size of the roots. The agronomic traits of the tobacco plants in each group are shown in table 1 and fig. 3.
TABLE 1 Effect of different inoculum treatments on agronomic traits of tobacco plants
It can be seen that the agronomic character of the tobacco strain applied with the Streptomyces spectabilis 69-1 fermentation broth is better than that of other groups.
Example 4 Effect of different microbial Agents on the fresh weight and Dry weight of Single leaf of tobacco leaves at the upper, middle and lower parts
Selecting 90 tobacco seedlings with the same growth condition, and averagely dividing the tobacco seedlings into three groups, wherein the first group is a blank control group without applied microbial inoculum, the second group is a bacillus subtilis wettable powder group, and the third group is a streptomyces spectabilis 69-1 fermentation broth; the first group does not contain any microbial inoculum, and the second group pours the bacillus subtilis wettable powder into a cultivation hole for 1 g/plant when crops are cultivated after the effective number of live spores of the bacillus subtilis wettable powder is more than or equal to 10.0 hundred million/g and root watering is carried out by water mixing; in the third group, the fermentation liquor of the streptomyces spectabilis 69-1 is mixed with watering water in any proportion and then poured into cultivation holes when crops are cultivated, and the dosage is 10ml per plant. The fresh weight and dry weight of each leaf of the upper, middle and lower tobacco of each group of tobacco plants are shown in Table 2.
TABLE 2 influence of different bacterial agents on fresh weight and dry weight of single leaf of tobacco leaves at upper, middle and lower parts
It can be seen that the tobacco strain to which the Streptomyces spectaculeatus 69-1 fermentation broth was applied had a single fresh weight per leaf higher than that of the second group, and all the fresh weights per leaf and dry weights of the other tobacco leaves were heavier than those of the other groups.
Example 5 Effect of different microbial inoculum treatments on tobacco diseases
Selecting 90 tobacco seedlings with the same growth condition, and averagely dividing the tobacco seedlings into three groups, wherein the first group is a blank control group without applied microbial inoculum, the second group is bacillus subtilis wettable powder, and the third group is bacterial powder prepared from streptomyces spectabilis 69-1 fermentation liquor; the first group does not contain any microbial inoculum; in the second group, when the bacillus subtilis wettable powder is used for cultivating plants, the dried powder is applied to the field, and the using amount is 1 g; and in the third group, the streptomyces spectabilis 69-1 fermentation liquor is added into the sterilized wheat bran, the mixture is dried at low temperature to prepare fungus powder, and the dried fungus powder is applied to the field when plants are cultivated, wherein the dosage is 100ml per plant. The influence of different microbial inoculum treatments on flue-cured tobacco diseases is shown in table 3.
TABLE 3 Effect of different inoculum treatments on tobacco diseases
| Treatment group | Incidence (%) | Index of disease condition | Relative control effect (%) |
| First group | 34.00±3.46 | 29.26±1.81 | -- |
| Second group | 25.33±2.31 | 21.48±1.03 | 26.54±1.13 |
| Third group | 14.67±1.51 | 11.56±0.59 | 60.42±2.82 |
It can be seen that the incidence and disease index of the third group of tobacco plants are significantly lower than those of the other two groups, and the relative control effect is significantly higher than that of the other groups.
EXAMPLE 6 Effect of different treatments on the chemical composition of flue-cured tobacco leaves
Selecting 90 tobacco seedlings with the same growth condition, and averagely dividing the tobacco seedlings into three groups, wherein the first group is a blank control group without applied microbial inoculum, the second group is bacillus subtilis wettable powder, and the third group is bacterial powder prepared from streptomyces spectabilis 69-1 fermentation liquor; the first group does not contain any microbial inoculum; in the second group, after bacillus subtilis wettable powder is activated in nutrient solution, the bacillus subtilis wettable powder is poured into roots of plants during cultivation, and the dosage is 1 g/plant; and in the third group, the fermentation liquor of the streptomyces spectabilis 69-1 is added with the sterilized wheat bran, the mixture is dried at low temperature to prepare fungus powder, and the fungus powder is activated in nutrient solution and poured into the roots of plants during cultivation, wherein the dosage is 50mL per plant. The effect of the different treatments on the chemical composition of the flue-cured tobacco leaves is shown in table 4.
TABLE 4 Effect of different treatments on the chemical composition of flue-cured tobacco leaves
| Treatment group | Total nitrogen (%) | Potassium oxide (%) | Total sugar (%) | Reducing sugar (%) | Nicotine (%) |
| First group | 2.37±0.03 | 3.17±0.03 | 21.82±0.03 | 14.17±0.09 | 2.13±0.01 |
| Second group | 2.13±0.04 | 2.46±0.05 | 23.09±0.07 | 15.48±0.12 | 2.06±0.02 |
| Third group | 2.25±0.06 | 3.08±0.03 | 21.37±0.07 | 11.07±0.05 | 2.12±0.02 |
As can be seen from table 4, the chemical composition of the three groups did not differ significantly.
EXAMPLE 7 Effect of different treatments on other chemical compositions of flue-cured tobacco leaves
Selecting 90 tobacco seedlings with the same growth condition, and averagely dividing the tobacco seedlings into three groups, wherein the first group is a blank control group without applied microbial inoculum, the second group is bacillus subtilis wettable powder, and the third group is streptomyces spectabilis 69-1 fermentation liquor, and freeze-drying the fermentation liquor to prepare pure bacterial powder; the first group does not contain any microbial inoculum; in the second group, the bacillus subtilis wettable powder is directly applied when plants are cultivated, and the dosage is 1 g per plant; freeze-drying the third group of Streptomyces spectabilis 69-1 fermentation liquor to prepare pure bacterial powder, and directly applying the pure bacterial powder with the dosage of 10ml per plant when cultivating plants. The effect of the different treatments on the other chemical components of the flue-cured tobacco leaves is shown in table 5. As shown in figure 3, the disease prevention effect of the Streptomyces spectaculeatus 69-1 in the continuous cropping soil is shown, wherein the effect of the control group without the microbial inoculum applied to the left behavior is shown in the figure, and the effect of the streptomyces spectaculeatus 69-1 applied to the right behavior is shown in the figure, so that the strain not only can prevent and control the occurrence of soil-borne diseases, but also can promote the growth of plants.
TABLE 5 Effect of different treatments on other chemical compositions of flue-cured tobacco leaves
The third group of other chemicals was significantly higher than the other groups except for starch content.
Claims (8)
1. A strain of Streptomyces spectabilisStreptomyces spectabilis)69-1, the deposit number is: CCTCC NO: and M2019974.
2. The application of the streptomyces spectabilis strain 69-1 of claim 1 in controlling tobacco black shank and bacterial wilt.
3. The use of a strain of Streptomyces spectaculeatus 69-1 according to claim 1 for promoting plant growth and development.
4. Use according to claim 2 or 3, characterized in that: the method comprises the steps of utilizing a Streptomyces spectabilis 69-1 fermentation liquor to prevent and control tobacco black shank and bacterial wilt and promote plant growth and development, wherein the Streptomyces spectabilis 69-1 fermentation liquor is obtained by inoculating a seed liquid of the Streptomyces spectabilis 69-1 to a potato-oat flour liquid culture medium for culture.
5. Use according to claim 4, characterized in that: the spore concentration in the Streptomyces spectabilis 69-1 fermentation liquor is 1 multiplied by 107-1×1012cfu/ml。
6. Use according to claim 4, characterized in that: the fermentation liquor of the streptomyces spectabilis 69-1 is prepared by the following method: inoculating the seed solution of the streptomyces spectabilis 69-1 to a potato-oat powder liquid culture medium, and culturing at the temperature of 28 ℃ for 80-180 r.min-1And culturing for 6-10 days by a shaking table to obtain the Streptomyces spectabilis 69-1 fermentation liquid, wherein the inoculation amount of the seed liquid is 1-10% of the volume of the potato-oat flour liquid culture medium.
7. Use according to claim 4, characterized in that: culturing the Streptomyces spectabilis 69-1 in a potato-oat flour liquid culture medium for 4-8 days to obtain a seed liquid of the Streptomyces spectabilis 69-1.
8. Use according to claim 4, characterized in that: the application method of the Streptomyces spectabilis 69-1 fermentation liquor comprises the following steps: A. when crops are cultivated, digging cultivation holes corresponding to the sizes of seedling roots in soil, placing seedlings, pouring the Streptomyces spectabilis 69-1 fermentation liquor on the roots of the plants, wherein the using amount of the Streptomyces spectabilis 69-1 fermentation liquor is 10ml-100ml per plant according to the sizes of the roots; or the like, or, alternatively,
B. mixing 10ml-100ml of fermentation liquor of Streptomyces spectabilis 69-1 with water for root watering in any proportion, and watering the mixture into a cultivation hole when crops are cultivated, or watering the mixture after soil covering; or the like, or, alternatively,
C. adding the fermentation liquor of the streptomyces spectabilis 69-1 into the sterilized wheat bran, drying at low temperature to prepare fungus powder, and applying 0.1-10 g of the dried fungus powder to a field when plants are cultivated; or the like, or a combination thereof,
D. adding Streptomyces spectabilis 69-1 fermentation liquor into sterilized wheat bran, drying at low temperature to obtain fungus powder, activating the fungus powder 0.1-10 g/strain in nutrient solution, and pouring into plant root during cultivation; or the like, or, alternatively,
E. freeze-drying Streptomyces spectabilis 69-1 fermentation liquor to prepare pure bacterial powder, and directly applying 0.01-1 g of the pure bacterial powder per plant when plants are cultivated; or the like, or, alternatively,
F. freeze-drying Streptomyces spectabilis 69-1 fermentation liquor to prepare pure bacterial powder, and mixing 0.01-1 g/strain of the pure bacterial powder with an organic fertilizer for application; or the like, or, alternatively,
G. freeze drying Streptomyces spectabilis 69-1 fermentation liquor to prepare pure bacterial powder, activating bacterial powder by 0.01-1 g/strain, and pouring bacterial liquid containing activated bacteria on the roots of plants.
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