CN112125407A - Method for treating pig manure wastewater by using microalgae and application - Google Patents
Method for treating pig manure wastewater by using microalgae and application Download PDFInfo
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- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The invention discloses a method for treating pig manure wastewater by using microalgae and application thereof, relating to the technical field of wastewater treatment, and the technical scheme is as follows: filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate; diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-25%; pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement; activation treatment before inoculation enables the algae to reach the optimal growth state; inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in spring, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 14-24 ℃, and the removal period is 5-7 days; and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid. Not only can improve the overall removal rate of ammonia nitrogen, total phosphorus and phosphate, but also improve the yield of microalgae, and simultaneously provides technical support for the preparation of pig manure wastewater and biodiesel.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a method for treating pig manure wastewater by using microalgae and application thereof.
Background
In recent years, due to the rapid development of the pig raising industry in China, a large amount of pig manure wastewater is generated, but the pig manure wastewater is not treated in time, so that the environment is polluted greatly. Compared with the traditional pig raising, the large-scale pig farm produces large amount of excrement, urine and sewage, and 80t of fresh excrement, 260t of sewage and NH are produced in a 10 ten thousand pig farm3-N about 159 kg/h. The pollution characteristics of the pig farm fecal sewage are shown as follows: the wastewater amount and impact load are large, solid and liquid are mixed, the concentrations of suspended matters and organic matters are high, the contents of ammonia nitrogen and phosphorus are high, the carbon-nitrogen ratio is unbalanced, the concentrations of nitrogen and phosphorus are 20-50 times of that of domestic sewage, and the treatment difficulty is very large; carry a large amount of pathogenic bacteria and emit a very strong odor.
At present, microalgae have application values in the aspects of nitrogen and phosphorus removal of wastewater, carbon emission reduction and the like. In the growth process of microalgae cells, organic nitrogen, phosphorus and other substances in the wastewater can be converted into self-needed high-energy organic compounds by using three forms of substrate level phosphorylation, oxidative phosphorylation and photo-phosphorylation, so that the content of the organic substances such as nitrogen, phosphorus and the like in the wastewater is effectively reduced. Canizares and the like prepare pig manure into nutrient solution, and the nutrient solution is used for culturing spirulina, and research shows that: on the 6 th day of culture, the biomass of spirulina reached the highest, the ammonia nitrogen removal rate reached 75%, and the total phosphorus and phosphate removal rates were 53% and 98%, respectively. Chang et al, cultured Haematococcus pluvialis with domestic sewage (subjected to primary treatment) and pig farm wastewater, and proved by research: when the waste water is diluted by 4 times, the growth amount of the haematocyte algae cultured by the waste water is equivalent to that of the haematocyte algae cultured by a common culture medium, and the nitrogen and phosphorus removal effect is good. Elizabeth and the like use microalgae to treat pig manure wastewater, researches the relation between the wastewater addition amount and the growth rate of the microalgae, and nutrient components, removal efficiency and the like, and shows that: when the addition amount of the wastewater is 0.40L/(m2 d), the algae growth amount is 9.4g/(m2 d), and the removal rates of nitrogen and phosphorus are respectively more than 90% and 68-78%. An and the like utilize the wastewater of the pig farm to culture the botryococcus braunii, and find that the botryococcus braunii grows well, and the maximum algal density is as high as 7.8 g/L. The researchers use the pig manure wastewater to cultivate energy microalgae, and have carried out a great deal of research work and obtained remarkable achievements.
However, the microalgae adapting to the pig manure wastewater environment, the growth rate, the nitrogen and phosphorus removal efficiency and the biomass yield are convenient to be improved. Therefore, intensive research on the method for treating the pig manure wastewater and the application thereof is a problem which is urgently needed to be solved at present.
Disclosure of Invention
The invention aims to provide a method for treating pig manure wastewater by using microalgae and application thereof, which can improve the overall removal rate of ammonia nitrogen, total phosphorus and phosphate, improve the yield of microalgae, provide technical support for preparation of pig manure wastewater and biodiesel, and have the characteristics of low cost, low energy consumption, low pollution, high efficiency, high benefit and the like compared with the traditional treatment method.
The technical purpose of the invention is realized by the following technical scheme:
in a first aspect, a method for treating pig manure wastewater by using microalgae is provided, which comprises the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-25%;
s3: pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at the rotating speed of 4000-5000r/min for 12-18min, removing the supernatant, taking the residual algae residue, adding a new BG-11 culture solution, and performing aeration culture for 24h under the conditions of the temperature of 20-25 ℃ and the illumination of 1200-1500lux to enable the algae seeds to reach the optimal growth state;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in spring, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 14-24 ℃, and the removal period is 5-7 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
Preferably, the method comprises the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-25%;
s3: pre-culturing algae seeds by BG-11 culture solution at the temperature of 25 ℃ under illumination of 1300lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at 4500r/min for 15min, removing supernatant, adding the rest algae residue into new BG-11 culture solution, and performing aerobic culture at 25 deg.C under 1300lux for 24 hr to obtain optimal growth state of algae;
s5: inoculating the inoculated algae seeds in the optimal growth state into a pig manure wastewater culture solution in a volume ratio of 1:10 in spring, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 20 ℃, and the removal period is 6 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
In a second aspect, a method for treating pig manure wastewater by using microalgae is provided, which comprises the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-15%;
s3: pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at the rotating speed of 4000-5000r/min for 12-18min, removing the supernatant, taking the residual algae residue, adding a new BG-11 culture solution, and performing aeration culture for 24h under the conditions of the temperature of 20-25 ℃ and the illumination of 1200-1500lux to enable the algae seeds to reach the optimal growth state;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in winter, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 1-13 ℃, and the removal period is 8-10 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
Preferably, the method comprises the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-15%;
s3: pre-culturing algae seeds by BG-11 culture solution at the temperature of 25 ℃ under illumination of 1300lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at 4500r/min for 15min, removing supernatant, adding the rest algae residue into new BG-11 culture solution, and performing aerobic culture at 25 deg.C under 1300lux for 24 hr to obtain optimal growth state of algae;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in winter, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 10 ℃, and the removal period is 9 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
Preferably, on the basis of the first aspect or the second aspect, the algal species is chlorella PCS.
Preferably, on the basis of the first aspect or the second aspect, the concentration of ammonia nitrogen in the filtrate of the pig manure wastewater is 160-200mg/L, the concentration of total nitrogen is 400-600mg/L, the concentration of total phosphorus is 30-50mg/L, and the concentration is 30-40 mg/L.
Preferably, in the first or second aspect, the BG-11 culture solution comprises: 200mg/L NaNO340mg/L of K2HPO4·3H2O, 75mg/L MgSO4·7H2O, 36mg/L CaCl2·2H2O, citric acid 6mg/L, ferric citrate 6mg/L, EDTA Na 1mg/L220mg/L of Na2CO3And trace elementsA5+Co。
Preferably, on the basis of the first aspect or the second aspect, the trace elements a5+ Co include: 2860mg/L of H3BO31800mg/L MnCl2·4H2O, 220mg/L ZnSO4·7H2O, 80mg/L CuSO4·5H2O。
In a third aspect, the application of the method for treating the pig manure wastewater by using the microalgae according to the first aspect or the second aspect in removing wastewater rich in ammonia nitrogen, total nitrogen, phosphate and total phosphorus is provided.
In a fourth aspect, the application of the method for treating pig manure wastewater by using microalgae according to the first aspect or the second aspect in the cultivation of energy microalgae is provided.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention combines the pig manure wastewater treatment with the large-scale culture of microalgae, thereby not only realizing low-cost discharge of the pig manure wastewater and reducing the discharge of harmful substances in the resource utilization process, but also saving the culture cost of the microalgae;
2. the method can improve the overall removal rate of ammonia nitrogen, total phosphorus and phosphate, also improve the yield of microalgae, and simultaneously provide technical support for the preparation of pig manure wastewater and biodiesel, and compared with the traditional treatment method, the method has the characteristics of low cost, low energy consumption, low pollution, high efficiency, high yield and the like;
3. the invention can carry out open type pig manure wastewater treatment and green ball algae PCS production in two seasons with large temperature difference during spring and winter, is not limited by climate and input cost, and has wide application prospect.
4. The preparation period of the pig manure wastewater and the biodiesel is short, and the working efficiency is improved.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.
FIG. 1 is a diagram illustrating the effect of the ammonia nitrogen concentration in the winter test in the embodiment of the present invention;
FIG. 2 is a graph showing the effect of total nitrogen concentration in winter experiments according to an embodiment of the present invention;
FIG. 3 is a graph showing the effect of total phosphorus concentration in winter experiments according to an embodiment of the present invention;
FIG. 4 is a graph showing the effect of the concentration of phosphate in the winter test in the example of the present invention;
FIG. 5 is a graph illustrating the effect of the removal rate of the winter test according to an embodiment of the present invention;
FIG. 6 is a diagram showing the effect of ammonia nitrogen concentration in spring experiments in the embodiment of the present invention;
FIG. 7 is a graph showing the effect of total nitrogen concentration in spring experiments according to an embodiment of the present invention;
FIG. 8 is a graph showing the effect of spring test total phosphorus concentration in the examples of the present invention;
FIG. 9 is a graph showing the effect of the concentration of phosphate in spring test in the examples of the present invention;
FIG. 10 is a graph showing the effect of the removal rate in the spring test according to the embodiment of the present invention.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the present invention more clearly understood, the present invention is further described in detail below with reference to fig. 1 to 10 and embodiments 1 to 2.
Example 1: a method for treating pig manure wastewater by using microalgae comprises the following steps:
and S1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate. The concentration of ammonia nitrogen in the filtrate of the pig manure wastewater is 160-200mg/L, the total nitrogen concentration is 400-600mg/L, the total phosphorus concentration is 30-50mg/L and the concentration is 30-40 mg/L.
S2: the filter liquor of the pig manure wastewater is diluted into a culture solution of the pig manure wastewater with the volume concentration of 5-25%.
S3: pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement. The algal species is chlorella PCS.
The pre-culture of the algae seeds comprises the following specific steps: preparing BG-11 solid culture medium, and pouring into a flat plate. Inoculating the algae seeds in the culture dish to the plate, and placing the plate in an incubator for culture. Then, 100mL of conical flask is taken, about 25mLBG-11 culture medium is added, a PCS plate with good growth is taken, a small amount of algae seeds are taken by an inoculating stick in a super-clean operation platform and inoculated into the conical flask, and the conical flask is placed at the temperature of 25 ℃ and the illumination of 1300lux for culture. Then, 2.5L of the culture flask was added with 1.5LBG-11 medium, PCS in the flask was inoculated into the culture flask in a clean bench, and the flask was placed at 25 ℃ under 1300lux of light for aeration and expanded culture.
S4: before inoculation, centrifuging the inoculated algae liquid at 4000-5000r/min for 12-18min, removing supernatant, adding the rest algae residue into new BG-11 culture solution, and culturing under the conditions of 20-25 deg.C and illumination of 1200-1500lux for 24 hr to make the algae seed reach the optimal growth state.
S5: in spring, inoculating the strain in optimal growth state into the culture solution of pig manure wastewater at a volume ratio of 1:10, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 14-24 ℃, and the removal period is 5-7 days.
S6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
PCS full name Chlorococcum pamirum, Chinese name Chlorococcum pamirum. Pamilfoil is a kind of prototheca unicellular green algae belonging to the class Chlorophyceae (Chlorophyceae) order Chlorococcales (Chlorococcales) order oocystis (Ocystaceae) genus (Chlorella).
PCS is wide in ecological distribution, generally mainly takes a fresh water area as a main part, and is high in growth and propagation speed, simple in culture process and large in biomass. Meanwhile, the algae can effectively utilize carbon dioxide (carbon source), light (energy source) and water to form carbon-rich grease, and can be converted into biodiesel through an ester exchange method. Importantly, biodiesel produced by PCS growth does not depend on basic production of food crops or feed and other products, and therefore many marginal lands can also be used for microalgae culture.
The specific components of BG-11 culture fluid are shown in Table 1:
TABLE 1 BG-11 culture solution specific composition
The specific components of the trace element A5+ Co culture solution are shown in Table 2:
Example 2: a method for treating pig manure wastewater by using microalgae comprises the following steps:
and S1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate. The concentration of ammonia nitrogen in the filtrate of the pig manure wastewater is 160-200mg/L, the total nitrogen concentration is 400-600mg/L, the total phosphorus concentration is 30-50mg/L and the concentration is 30-40 mg/L.
S2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-15%;
s3: pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement. The algal species is chlorella PCS. BG-11 culture solution composition and algal species preculture method were the same as in example 1.
S4: before inoculation, centrifuging the inoculated algae liquid at the rotating speed of 4000-5000r/min for 12-18min, removing the supernatant, taking the residual algae residue, adding a new BG-11 culture solution, and performing aeration culture for 24h under the conditions of the temperature of 20-25 ℃ and the illumination of 1200-1500lux to enable the algae seeds to reach the optimal growth state;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in winter, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 1-13 ℃, and the removal period is 8-10 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
Experimental verification and analysis:
detection item and detection method
Detecting items: ammonia Nitrogen (NH)4-N), Total Nitrogen (TN), Phosphate (PO)4-P), Total Phosphorus (TP).
The determination method comprises the following steps: respectively culturing for 0, 3, 6, 9, 12 and 15 days, filtering appropriate amount of culture water with 0.45 μm cellulose acetate membrane, measuring N, P content, measuring NH4-N by Nashi reagent photometry, measuring TN by potassium persulfate-ultraviolet spectrophotometry, measuring TP by potassium sulfate digestion, measuring PO by molybdenum-antimony anti-spectrophotometry, and measuring4The determination of-P was carried out by molybdenum-antimony anti-spectrophotometry, in particular according to the analysis method for monitoring water and wastewater (fourth edition).
Second, raw water concentration selection of pig manure wastewater
The concentrations of ammonia nitrogen, total phosphorus and phosphate of the raw water of the pig manure wastewater for the experiment are respectively 182.2mg/L, 490.2mg/L, 40.4mg/L and 35.2 mg/L.
Third, design of experiment
In the experiment, 5 glass culture jars of 65L are arranged. The glass culture jar has length of 100cm, height of 65cm, and width of 10 cm. The culture volume of each glass culture tank is 50L.
The 5 glass culture cylinder tubes are numbered 1, 2, 3, 4 and 5 respectively. No. 1 culture tank is BG-11 culture medium as control; no. 2 culture tank uses pig manure wastewater diluted to 5% volume concentration; the No. 3 culture tank uses pig manure wastewater diluted to 10% volume concentration; no. 4 culture tank uses pig manure wastewater diluted to 15% volume concentration; no. 5 culture tank uses pig manure wastewater diluted to 25% volume concentration. Experiments were performed by the treatment methods in example 1 and example 2, respectively.
5 glass culture tubes were inoculated with 5L of Chlorococcum pamirum each. The absorbance of the inoculated algae solution at 550nm is about 3, and the initial absorbance OD after inoculation according to the volume ratio is about 0.3. 5 glass culture pots are placed on the roof for 24h aeration culture. Before extracting the solution, stirring the PCS and the pig manure wastewater uniformly, then extracting a proper volume of solution, extracting a proper volume of solution every 3 days, and then measuring the contents of ammonia nitrogen, total phosphorus and phosphate.
Fourth, winter experimental results and analysis
The temperature and weather conditions during the winter experiments are shown in table 3.
TABLE 3 temperature and weather conditions during the winter experimental trial
(1) The ammonia nitrogen measurement and the analysis of the results are shown in table 4 and fig. 1.
TABLE 4 Ammonia Nitrogen concentration in winter experiments
The ammonia nitrogen concentrations of the 5%, 10%, 15% and 25% pig manure wastewater are respectively 18.39mg/L, 36.78mg/L, 56.23mg/L and 92.53mg/L, wherein the removal rates of the 5% and 10% pig manure wastewater are 100%, the removal rate of the 15% pig manure wastewater is 72.38% and the removal rate of the 25% pig manure wastewater is only 37.75%. The probable reasons are analyzed, the excessive ammonia nitrogen content of the 25% pig manure wastewater has a toxic effect on PCS, the growth of PCS is not utilized, the temperature is not high, PCS cannot adapt to the pig manure wastewater with high concentration in a short time, the pig manure wastewater with 15% concentration and 25% concentration has dark color, the photosynthesis of PCS is influenced, and finally the removal rate of ammonia nitrogen is low due to the death of PCS, so that the comprehensive result is obtained, and PCS has a very good removal effect on ammonia nitrogen in the pig manure wastewater with proper concentration.
(2) The measurement of nitrogen (TN) and the analysis of the results are shown in table 5 and fig. 2.
TABLE 5 Total Nitrogen concentration for winter experiments
The total nitrogen concentration of the pig manure wastewater with the concentration of 5%, 10%, 15% and 25% is 31.91mg/L, 63.91mg/L, 94.85mg/L and 154.42mg/L respectively, and the removal rate is 97.65%, 81.36%, 72.36% and 71.37% respectively. Wherein the removal rate of the pig manure wastewater with the concentration of 5% is highest, the removal rate of the pig manure wastewater with the concentration of 10% is relatively higher, and the removal rates of the pig manure wastewater with other concentrations are more than 70%. The PCS of the pig manure wastewater with the concentration of 5% grows best, which shows that the concentration is more suitable for the growth of the PCS and has better removal effect on the total nitrogen in the pig manure wastewater. However, the removal effect of the pig manure wastewater with the concentration of 15% and 25% is obvious only in the first few days, and the later growth of PCS is not suitable, so that the growth of PCS is basically not high, and the removal effect of the pig manure wastewater with the concentration of 5% is not good.
(3) The measurement and analysis of the results of Total Phosphorus (TP) are shown in table 6 and fig. 3.
TABLE 6 Total phosphorus concentration for winter experiments
The total phosphorus concentrations of the pig manure wastewater with the concentrations of 5%, 10%, 15% and 25% are respectively 2.22mg/L, 4.42mg/L, 6.71mg/L and 11.04mg/L, and the removal rates are respectively 100%, 99.45%, 94.30% and 53.45%. It can be known that the PCS has the total phosphorus removal rate higher than 90% for the pig manure wastewater with the concentration of 5%, 10%, 15%, wherein the removal rate of the pig manure wastewater with the concentration of 5% and 15% is close to 100%, and the total phosphorus removal rate is almost the same on the 9 th day. However, the 10%, 15%, 25% concentration of pig manure wastewater after day 9 showed a small increase in the amount of wastewater, probably due to the death of some PCS, releasing absorbed, unconsumed total phosphorus. But it can be seen that PCS has a good effect of removing total phosphorus in the pig manure wastewater.
(4) The measurement and analysis of the results of phosphate are shown in table 7 and fig. 4.
TABLE 7 winter test phosphate concentration
The phosphate concentrations of the pig manure wastewater with the concentrations of 5%, 10%, 15% and 25% are respectively 1.8mg/L, 3.65mg/L, 5.44mg/L and 8.98mg/L, and the removal rates are respectively 100%, 95.48%, 93.74% and 60.14%. Therefore, the removal rate of phosphate in the pig manure wastewater with the concentration of 5%, 10% and 15% is higher than 90%, and a very good removal effect is achieved; the removal rate of 25% concentration pig manure wastewater is only 60%, probably the high concentration pig manure wastewater has inhibition effect on the growth of PCS, the temperature is not high during the test, PCS cannot adapt to the high concentration pig manure wastewater in a short time, the color of the high concentration pig manure wastewater deeply influences the photosynthesis of PCS, and after 9 days, PCS begins to die greatly, so that phosphate which is not consumed in algae is released, and the content of phosphate measured later is slightly increased. The graph shows that PCS has a relatively obvious effect of removing phosphate in low-concentration pig manure wastewater.
(5) N, P the results and analysis of the removal rate are shown in Table 8 and FIG. 5.
As can be seen from Table 8 and FIG. 5, PCS has very good removal effects on ammonia nitrogen, total phosphorus and phosphate in 5%, 10% and 15% of pig manure wastewater, and the removal rates are almost all higher than 90%, wherein the removal rates on ammonia nitrogen and total nitrogen are respectively higher than 97% and 95%. The removal rate of the PCS to ammonia nitrogen, total phosphorus and phosphate in the pig manure wastewater with the concentration of 5% and 10% is integrally higher than that of the pig manure wastewater with the concentration of 15% and 25%, wherein the pig manure wastewater with the concentration of 5% is the best in removal effect, and the pig manure wastewater with the concentration of 25% is relatively poor in removal effect.
TABLE 8 winter test removal
Fifth, spring experiment results and analysis
The temperature and weather conditions during the spring test are shown in table 9.
TABLE 9 temperature and weather conditions during the spring test
(1) The measurement of ammonia nitrogen and the analysis of the results are shown in Table 10 and FIG. 6.
TABLE 10 spring test Ammonia Nitrogen concentration
The ammonia nitrogen concentrations of 5%, 10%, 15% and 25% of the pig manure wastewater are respectively 9.11mg/L, 18.12mg/L, 27.40mg/L and 45.52mg/L, and the removal rates are respectively 97.59%, 98.23%, 98.47% and 97.32%. Therefore, the pig manure wastewater with four different concentrations has good removal effect; table 15 shows that the 5%, 10% and 15% pig manure wastewater was removed almost at day 6, while the 25% pig manure wastewater was slightly higher in concentration, and PCS was adapted to this high concentration, resulting in slower growth of PCS at the early stage compared to other low concentrations, but with the removal of PCS, the ammonia nitrogen concentration was reduced, so that ammonia nitrogen was again largely consumed at the later stage. On the whole, the temperature is more suitable for the growth of PCS under the condition of more than 20 ℃ during the experiment, the growth and the reproduction speed of PCS are high, and ammonia nitrogen in the pig manure wastewater is greatly consumed in a short time, so the ammonia nitrogen removal rate of the pig manure wastewater with 4 concentrations is higher, which shows that the PCS has a very good removal effect on the ammonia nitrogen under the condition of suitable temperature and ammonia nitrogen concentration.
(2) The measurement of Total Nitrogen (TN) and the analysis of the results are shown in table 11 and fig. 7.
TABLE 11 spring test Total Nitrogen concentration
The total nitrogen concentration of the 5%, 10%, 15% and 25% pig manure wastewater is 24.51mg/L, 49.14mg/L, 73.40mg/L and 122.30mg/L respectively, and the removal rate is 95.40%, 96.85%, 95.93% and 93.70% respectively. The removal rate is very high and is more than 90%, wherein the removal rate of the total nitrogen in the pig manure wastewater with the concentration of 10% is as high as 96.85%, the pig manure wastewater with each concentration is consumed by more total nitrogen in the first 6 days, because the concentration is relatively proper, the temperature is kept at more than 20 ℃, the weather is good, the PCS is propagated in large quantities, and further a large amount of total nitrogen is consumed, so that the PCS has a very good removal effect on the total nitrogen.
(3) The measurement of Total Phosphorus (TP) and the analysis of the results are shown in table 12 and fig. 8.
TABLE 12 spring test Total phosphorus concentration
The total phosphorus concentrations of the pig manure wastewater with the concentrations of 5%, 10%, 15% and 25% are respectively 2.02mg/L, 4.04mg/L, 6.49mg/L and 10.11mg/L, and the removal rates are respectively 96.59%, 98.14%, 98.72% and 98.89%. The removal rate of the 10%, 15% and 25% concentration pig manure wastewater is higher than 98%, so that a very good removal effect is shown, the removal rate of the 5% concentration pig manure wastewater also reaches 96%, and the removal effect is very good. The pig manure wastewater with 4 concentrations is greatly consumed by total phosphorus in the first 6 days, and the condition is caused because the temperature is more suitable for the growth of PCS in the first 6 days, and the concentration of the total phosphorus is more moderate, and PCS can adapt to the pig manure wastewater with various concentrations in a short time, so that mass propagation is carried out, and a large amount of total phosphorus is consumed. Later, because the PCS grows in a large amount in the early stage, the total phosphorus content is less, the total phosphorus consumption in the later days is relatively less, but the PCS has a very good effect of removing the total phosphorus in the pig manure wastewater.
(4) The measurement and result analysis of phosphate are shown in table 13 and fig. 9.
TABLE 13 spring test phosphate concentration
The phosphate concentrations of the pig manure wastewater with the concentrations of 5%, 10%, 15% and 25% are respectively 1.76mg/L, 3.76mg/L, 6.27mg/L and 9.89mg/L, and the removal rates are respectively 96.04%, 96.78%, 96.92% and 97.33%. The removal rate of phosphate in the pig manure wastewater with the concentration of 5%, 10%, 15% and 25% is higher than 96%, and the phosphate in the pig manure wastewater with the concentration of 4 kinds of concentrations is consumed in a large amount in the first 6 days, so that the situation is caused because the temperature is relatively suitable for the growth of PCS in the first 6 days, and the concentration of the phosphate is relatively moderate, and the PCS can adapt to the pig manure wastewater with various concentrations in a short time, so that mass propagation is carried out, and a large amount of phosphate is consumed. Later, because the PCS grows in large quantity in the early stage, but the phosphate content is lower, the phosphate consumption in the next few days is relatively less, but the PCS has good removal effect on the phosphate in the pig manure wastewater with proper concentration as a whole.
(5) The results of the removal rate of spring N, P are shown in table 14 and fig. 10.
TABLE 14 removal rate in spring experiment
As can be seen from table 14 and fig. 10, PCS has very good removal effects on ammonia nitrogen, total phosphorus and phosphate in pig manure wastewater with concentrations of 5%, 10%, 15% and 25%, the removal rates are almost all higher than 95%, and the removal rate of total nitrogen in pig manure wastewater with a concentration of only 25% is about 93%, but the removal effects are also very obvious. The removal rate of the PCS to the ammonia nitrogen, the total phosphorus and the phosphate in the pig manure wastewater with the concentration of 10% and 15% is integrally higher than that of the pig manure wastewater with the concentration of 5% and 25%, wherein the pig manure wastewater with the concentration of 10% is the best in removal effect, and the pig manure wastewater with the concentration of 5% is relatively poor in removal effect.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (10)
1. A method for treating pig manure wastewater by using microalgae is characterized by comprising the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-25%;
s3: pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at the rotating speed of 4000-5000r/min for 12-18min, removing the supernatant, taking the residual algae residue, adding a new BG-11 culture solution, and performing aeration culture for 24h under the conditions of the temperature of 20-25 ℃ and the illumination of 1200-1500lux to enable the algae seeds to reach the optimal growth state;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in spring, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 14-24 ℃, and the removal period is 5-7 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
2. The method for treating the pig manure wastewater by using the microalgae according to claim 1, which comprises the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-25%;
s3: pre-culturing algae seeds by BG-11 culture solution at the temperature of 25 ℃ under illumination of 1300lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at 4500r/min for 15min, removing supernatant, adding the rest algae residue into new BG-11 culture solution, and performing aerobic culture at 25 deg.C under 1300lux for 24 hr to obtain optimal growth state of algae;
s5: inoculating the inoculated algae seeds in the optimal growth state into a pig manure wastewater culture solution in a volume ratio of 1:10 in spring, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 20 ℃, and the removal period is 6 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
3. A method for treating pig manure wastewater by using microalgae is characterized by comprising the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-15%;
s3: pre-culturing algae seeds by BG-11 culture solution at 20-25 deg.C under illumination of 1200-1500lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at the rotating speed of 4000-5000r/min for 12-18min, removing the supernatant, taking the residual algae residue, adding a new BG-11 culture solution, and performing aeration culture for 24h under the conditions of the temperature of 20-25 ℃ and the illumination of 1200-1500lux to enable the algae seeds to reach the optimal growth state;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in winter, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 1-13 ℃, and the removal period is 8-10 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
4. The method and the application of the microalgae for treating the pig manure wastewater as claimed in claim 1, wherein the method comprises the following steps:
s1, filtering the original pig manure wastewater to obtain a pig manure wastewater filtrate;
s2: diluting the pig manure wastewater filtrate into a pig manure wastewater culture solution with the volume concentration of 5-15%;
s3: pre-culturing algae seeds by BG-11 culture solution at the temperature of 25 ℃ under illumination of 1300lux, wherein the absorbance OD of the algae solution under illumination of 550nm wavelength is 3 to meet the inoculation requirement;
s4: before inoculation, centrifuging the inoculated algae liquid at 4500r/min for 15min, removing supernatant, adding the rest algae residue into new BG-11 culture solution, and performing aerobic culture at 25 deg.C under 1300lux for 24 hr to obtain optimal growth state of algae;
s5: inoculating the inoculated algae seeds in the optimal growth state into the pig manure wastewater culture solution in a volume ratio of 1:10 in winter, wherein the initial absorbance OD after inoculation is about 0.3, the removal temperature is 10 ℃, and the removal period is 9 days;
s6: and (4) centrifugally collecting the microalgae in the pig manure wastewater removal liquid.
5. The method for treating pig manure wastewater by using microalgae as claimed in claim 1 or 3, wherein the algal species is Chlorococcus PCS.
6. The method and application of claim 1 or 3, wherein the concentration of ammonia nitrogen in the pig manure wastewater filtrate is 160-200mg/L, the total nitrogen concentration is 400-600mg/L, the total phosphorus concentration is 30-50mg/L, and the concentration is 30-40 mg/L.
7. The method and application of claim 1 or 3, wherein the BG-11 culture solution comprises: 200mg/L NaNO340mg/L of K2HPO4·3H2O, 75mg/L MgSO4·7H2O, 36mg/L CaCl2·2H2O, citric acid 6mg/L, ferric citrate 6mg/L, EDTA Na 1mg/L220mg/L of Na2CO3And trace elements a5+ Co.
8. The method and application of claim 7, wherein the trace elements A5+ Co comprise: 2860mg/L of H3BO31800mg/L MnCl2·4H2O, 220mg/L ZnSO4·7H2O, 80mg/L CuSO4·5H2O。
9. The use of the method of any one of claims 1 to 8 for the treatment of pig manure wastewater with microalgae for the removal of wastewater rich in ammonia nitrogen, total nitrogen, phosphate and total phosphorus.
10. The use of the method of any one of claims 1 to 8 for treating swine waste water with microalgae for cultivating energy microalgae.
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