CN112114061A - Method for detecting taurine in formula food with special medical application - Google Patents
Method for detecting taurine in formula food with special medical application Download PDFInfo
- Publication number
- CN112114061A CN112114061A CN202010741833.9A CN202010741833A CN112114061A CN 112114061 A CN112114061 A CN 112114061A CN 202010741833 A CN202010741833 A CN 202010741833A CN 112114061 A CN112114061 A CN 112114061A
- Authority
- CN
- China
- Prior art keywords
- sample
- taurine
- solution
- supernatant
- standard
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/36—Control of physical parameters of the fluid carrier in high pressure liquid systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8679—Target compound analysis, i.e. whereby a limited number of peaks is analysed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Library & Information Science (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention discloses a method for detecting taurine in formula food with special medical application, which relates to the technical field of food detection and comprises the following steps: firstly, preparing a standard solution; preparing a sample; finally analyzing the result; the invention dissolves the sample by water, and uses potassium ferrocyanide and zinc acetate to precipitate the protein; and (3) carrying out derivatization reaction on the supernatant by using dansyl chloride, separating the derivative by using a C18 reversed phase chromatographic column, and finally detecting the content of taurine in the formula food with special medical application by using a detector.
Description
Technical Field
The invention relates to the technical field of food detection, in particular to a method for detecting taurine in formula food with special medical application.
Background
The special food is specially processed according to a special formula in order to meet the physiological needs of certain special people or the nutritional needs of certain patients.
Taurine is a special amino acid, is the most abundant in marine shellfish and is an essential nutrient element for human body. It has effects of resisting atherosclerosis, and preventing hyperlipemia and cerebral thrombosis. The content of taurine is 50 percent of amino acid in the heart, has obvious protective effect on ischemic myocardial cells, inhibits the occurrence of arrhythmia, and provides a new method for treating viral myocarditis.
In the prior art, compared with the method provided by the national standard, the method can improve the sensitivity and selectivity of detection, but the detection limit of the method is still to be improved, and the method has single substrate and component of functional beverage and food, has few impurities and can not adapt to the detection of taurine in special food.
Therefore, the detection method with low detection limit, high accuracy and easy control is sought to solve the problem urgently.
Disclosure of Invention
The invention provides a method for detecting taurine in formula food with special medical application.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for detecting taurine in formula food with special medical application comprises the following steps:
(1) preparing a standard solution:
preparing 1mg/mL taurine standard stock solution: accurately weighing 0.1000g of taurine standard substance, dissolving with water and fixing the volume to 100 mL;
preparing a taurine standard working solution: diluting the taurine standard stock solution with water to prepare a series of standard solutions, wherein the concentrations of the standard solutions are respectively 2.0 mug/mL, 5.0 mug/mL, 10.0 mug/mL, 20.0 mug/mL and 50.0 mug/mL;
(2) preparing a sample:
firstly, extracting a test solution:
accurately weighing 2.5g of a solid sample into a centrifuge tube, adding 20mL of warm water at about 40 ℃, shaking uniformly to dissolve the sample, and putting the sample into an ultrasonic oscillator for ultrasonic extraction for 10 min; cooling to room temperature, adding 0.5mL of precipitator I, mixing by vortex, adding 0.5mL of precipitator II, mixing by vortex, centrifuging the sample liquid at 5000r/min for 10min, and taking the supernatant liquid for later use;
accurately weighing a liquid sample which does not contain a milk beverage sample, putting 5-30 g of the liquid sample into a conical flask, adding 20mL of water, and fully shaking up; adding 1.0mL of precipitator I, mixing by vortex, adding 1.0mL of precipitator II, mixing by vortex, transferring into a 100mL volumetric flask, adding water to a constant volume to a scale, and fully and uniformly mixing; centrifuging the sample solution at 5000r/min for 10min, and collecting the supernatant II for use;
weighing 5 g-40 g of milk beverage sample, putting the sample into a conical flask, adding 20mL of warm water at 40 ℃, fully mixing, placing on an ultrasonic oscillator for ultrasonic extraction for 10min, and cooling to room temperature; adding 1.0mL of precipitator I, carrying out vortex mixing on 1.0mL of precipitator II, transferring into a 100mL volumetric flask, adding water to a constant volume to a scale, fully and uniformly mixing, centrifuging sample liquid at 5000r/min for 10min, and taking supernatant liquid for later use;
in carrying out the derivatization in the test solution:
accurately sucking 1.00mL of supernatant I, supernatant II and supernatant III into a glass test tube with a plug respectively, adding 1.00mL of sodium carbonate buffer solution and 1.00mL of dansyl chloride solution, fully mixing, carrying out derivatization reaction at room temperature in a dark place for 2 hours, shaking the derivatization reaction for 1 time every 1 hour, adding 0.10mL of methylamine hydrochloride solution, carrying out vortex mixing to terminate the reaction, and standing in the dark place until the precipitation is complete; filtering the supernatant fluid four by a 0.45 mu m microporous filter membrane, and taking the filtrate for later use;
taking another 1.00mL of taurine standard working solution, and synchronously derivatizing with the test solution, wherein the step is consistent with the step of derivatization;
(3) and (3) analysis results:
respectively injecting derivative solutions of the standard series working solutions into a high performance liquid chromatograph, measuring corresponding chromatographic peak height or peak area, and drawing a standard curve by taking the concentration of the standard working solutions as a horizontal coordinate and taking response values as a vertical coordinate;
the taurine content of the sample is calculated according to the following formula:
A＝C×V/m/1000×100
in the formula:
a-taurine content in milligrams per hundred grams (mg/100g) of sample;
c-concentration of taurine in the sample measurement solution in units of micrograms per milliliter
(μg/mL)；
V is the volumetric volume of the sample in milliliters (mL);
m is the mass of the sample in grams (g).
Further, before all steps are carried out, 1mol/L hydrochloric acid solution, 80mmol/L sodium carbonate buffer solution, 1.5mg/mL dansyl chloride solution, 20mg/L methylamine hydrochloride solution and 10mmol/L sodium acetate buffer solution are required to be distributed and prepared;
further, the preparation method of the precipitating agent I comprises the following steps: weighing 15.0g of potassium ferrocyanide, dissolving with water and fixing the volume to 100 mL; the precipitant is stable at room temperature for 3 months;
further, the preparation method of the precipitator II comprises the following steps: weighing 30.0g of zinc acetate, dissolving with water and fixing the volume to 50 mL; the precipitant remains stable for 3 months at room temperature;
further, the supernatant I, the supernatant II and the supernatant III in the step (2) are all stable within 24 hours after being stored at 4 ℃ in the dark.
Further, the derivative generated in the derivatization of the test solution can be stored for 48 hours at the temperature of below 4 ℃ in the dark.
The invention has the following advantages: the invention dissolves the sample by water, and precipitates the protein by potassium ferrocyanide and zinc acetate; and (3) carrying out derivatization reaction on the supernatant by using dansyl chloride, separating the derivative by using a C18 reversed phase chromatographic column, and finally detecting the content of taurine in the formula food with special medical application by using a detector.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
A method for detecting taurine in formula food with special medical application comprises the following steps:
the principle is as follows:
the sample was dissolved in water and the protein was precipitated with potassium ferrocyanide and zinc acetate. Collecting supernatant, derivatizing with dansyl chloride, and subjecting the derivative to reaction C18Separating with reverse phase chromatographic column. Detecting with ultraviolet detector (254nm) or fluorescence detector (excitation wavelength: 330 nm; emission wavelength: 530nm), and quantifying by external standard method.
Reagents and materials:
unless otherwise stated, all reagents used in the method are analytically pure, and water is primary water specified in GB/T6682. Acetonitrile: and (4) carrying out chromatographic purification. Glacial acetic acid. Hydrochloric acid. Anhydrous sodium carbonate. And (3) sodium acetate. Methylamine hydrochloride (methylamine hydrochloride). Dansyl chloride (5-dimethylaminonaphthalene 1-sulfonyl chloride): and (4) carrying out chromatographic purification. The dansyl chloride is not sensitive to light and moisture and is preserved in a desiccator without light.
Preparing a reagent:
hydrochloric acid solution (1 mol/L): 9mL of hydrochloric acid (2.1.3) (100mL beaker) was aspirated, diluted with water and brought to 100mL (100mL volumetric flask).
Sodium carbonate buffer (pH 9.5) (80 mmol/L): 0.424g of anhydrous sodium carbonate (2.1.4) was weighed out, dissolved in 40mL of water (50mL beaker), adjusted to pH 9.5 with 1mol/L hydrochloric acid solution (2.2.1) and made up to 50mL with water (50mL volumetric flask). The solution was stable for 3 months at room temperature.
Dansyl chloride solution (1.5 mg/mL): 0.15g dansyl chloride (2.1.7) (50mL beaker) was weighed, dissolved with acetonitrile (2.1.1) and made to volume of 100mL (100mL volumetric flask). It is prepared just before use.
Methylamine hydrochloride solution (20 mg/L): 2.0g methylamine hydrochloride (2.1.6) (50mL beaker) was weighed, dissolved with water and made to volume of 100mL (100mL volumetric flask). The solution was stable for 3 months when stored at 4 ℃.
Sodium acetate buffer (10mmol/L, pH 4.2): 0.820g of sodium acetate (2.1.5) (1000mL beaker) was weighed out, dissolved in 800mL of water, adjusted to pH 4.2 with glacial acetic acid (2.1.2), made up to 1000mL (1000mL volumetric flask) with water and filtered through a 0.45 μm microporous membrane.
The purity of the standard substance is more than or equal to 99 percent, and the CAS: 107-35-7.
Preparing a standard solution:
taurine standard stock solution (1 mg/mL): 0.1000g of taurine standard (50mL beaker) was weighed out accurately, dissolved in water and made up to 100mL (100mL volumetric flask).
Taurine standard working solution (for uv detector): a series of standard solutions were prepared by diluting taurine standard stock solutions (2.4.1) with water, the standard concentrations being 2.0. mu.g/mL, 5.0. mu.g/mL, 10.0. mu.g/mL, 20.0. mu.g/mL, 50.0. mu.g/mL, and prepared immediately before use.
Instruments and devices:
high performance liquid chromatograph: with a fluorescence detector or an ultraviolet detector or a diode array detector. Vortex the mixer. An ultrasonic oscillator. A pH meter: the precision is 0.01. A centrifugal machine: not less than 5000 r/min. Microporous filter membrane: 0.45 μm. Balance: 0.0001g and 0.001 g.
And (3) an analysis step:
sample preparation, sample liquid extraction:
accurately weighing 2.5g (accurate to 0.01g) of solid sample into a centrifuge tube (50mL), adding 20mL of warm water at about 40 ℃, shaking uniformly to dissolve the sample, and placing the sample into an ultrasonic oscillator for ultrasonic extraction for 10 min. Cooling to room temperature, adding 0.5mL of precipitant I (precipitant I: 15.0g potassium ferrocyanide is weighed, dissolved with water and constant volume to 100mL, the precipitant is stable at room temperature for 3 months), mixing by vortex, adding 0.5mL of precipitant II (precipitant II: 30.0g zinc acetate is weighed, dissolved with water and constant volume to 50mL, the precipitant is stable at room temperature for 3 months), mixing by vortex, centrifuging the sample solution at 5000r/min for 10min, and taking the supernatant for later use. The supernatant was stored at 4 ℃ in the dark and stabilized within 24 h.
Accurately weighing 5 g-30 g (accurate to 0.01g) of liquid sample (except milk beverage sample) in a conical flask, adding 20mL of water, and fully shaking up. Adding 1.0mL of precipitator I, mixing by vortex, adding 1.0mL of precipitator II, mixing by vortex, transferring into a 100mL volumetric flask, adding water to constant volume to scale, and mixing completely. Centrifuging the sample solution at 5000r/min for 10min, and collecting the supernatant. The supernatant was stored at 4 ℃ in the dark and stabilized within 24 h.
Weighing 5-40 g of sample (accurate to 0.01g) of milk beverage sample, adding 20mL of warm water at 40 ℃ into a conical flask, fully and uniformly mixing, placing on an ultrasonic oscillator for ultrasonic extraction for 10min, and cooling to room temperature. Adding 1.0mL of precipitator I, carrying out vortex mixing on 1.0mL of precipitator II, transferring into a 100mL volumetric flask, adding water to a constant volume to a scale, fully and uniformly mixing, centrifuging the sample liquid at 5000r/min for 10min, and taking the supernatant for later use. The supernatant was stored at 4 ℃ in the dark and stabilized within 24 h.
Derivatization of test solutions
Accurately sucking 1.00mL of the supernatant obtained by 4.1.1 into a 10mL glass test tube with a plug, adding 1.00mL of sodium carbonate buffer solution (2.2.2) and 1.00mL of dansyl chloride solution (2.2.3), fully mixing, carrying out derivatization reaction at room temperature in the dark for 2h (shaking for 1 time after 1 h), adding 0.10mL of methylamine hydrochloride solution (2.2.4), carrying out vortex mixing to terminate the reaction, and standing in the dark until the precipitate is complete. Filtering the supernatant with 0.45 μm microporous membrane (3.6), and collecting the filtrate. The derivative can be stored at below 4 deg.C in dark for 48 h.
Another 1.00mL standard working solution (2.4.2) was taken and derivatized simultaneously with the test solution.
Instrument reference conditions:
a chromatographic column: c18Reversed phase chromatography column (250 mm. times.4.6 mm, 5 μm) or equivalent. Mobile phase: sodium acetate buffer (2.2.5) + acetonitrile (2.1.1) ═ 75+25 (volume ratio), PH adjusted to 7.5. Flow rate of mobile phase: 1.0 mL/min. Column temperature: and (4) room temperature. Detection wavelength: ultraviolet detector or diode array detector: 290 nm. Sample introduction amount: 20 μ L.
And (3) preparing a standard curve:
and respectively injecting derivative solutions of the standard series working solutions into a high performance liquid chromatograph, measuring corresponding chromatographic peak heights or peak areas, and drawing a standard curve by taking the concentration of the standard working solution as a horizontal coordinate and taking a response value (peak area or peak height) as a vertical coordinate.
Measurement of sample solution:
and injecting the sample solution into a high performance liquid chromatograph to obtain the chromatographic peak height or peak area, and obtaining the concentration of the taurine in the liquid to be detected according to the standard curve.
Presentation of the results of the analysis:
the taurine content in the sample is calculated according to the formula (2):
A＝C×V/m/1000×100
in the formula:
a-taurine content in milligrams per hundred grams (mg/100g) of sample;
c-concentration of taurine in units of micrograms per milliliter (. mu.g/mL) in the test sample assay;
v is the volumetric volume of the sample in milliliters (mL);
m is the mass of the sample in grams (g).
The calculations are expressed as the arithmetic mean of two independent measurements obtained under repetitive conditions, with the results remaining three significant digits.
Precision:
the absolute difference between two independent measurements obtained under repetitive conditions must not exceed 10% of the arithmetic mean.
And others:
when the sampling amount is 10.00g, the detection limit of the fluorescence detection method is 0.05mg/100 g; the quantitative limit is as follows: 0.1mg/100 g. The detection limit of the ultraviolet detection method is 1.5mg/100 g; the limit of quantification is: 5mg/100 g.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention should be covered by the scope of the present invention.
Claims (6)
1. A method for detecting taurine in formula food with special medical application is characterized in that: the method comprises the following steps:
(1) preparing a standard solution:
preparing 1mg/mL taurine standard stock solution: accurately weighing 0.1000g of taurine standard substance, dissolving with water and fixing the volume to 100 mL;
preparing a taurine standard working solution: diluting the taurine standard stock solution with water to prepare a series of standard solutions, wherein the concentrations of the standard solutions are respectively 2.0 mug/mL, 5.0 mug/mL, 10.0 mug/mL, 20.0 mug/mL and 50.0 mug/mL;
(2) preparing a sample:
firstly, extracting a test solution:
accurately weighing 2.5g of a solid sample into a centrifuge tube, adding 20mL of warm water at about 40 ℃, shaking uniformly to dissolve the sample, and putting the sample into an ultrasonic oscillator for ultrasonic extraction for 10 min; cooling to room temperature, adding 0.5mL of precipitator I, mixing by vortex, adding 0.5mL of precipitator II, mixing by vortex, centrifuging the sample liquid at 5000r/min for 10min, and taking the supernatant liquid for later use;
accurately weighing a liquid sample which does not contain a milk beverage sample, putting 5-30 g of the liquid sample into a conical flask, adding 20mL of water, and fully shaking up; adding 1.0mL of precipitator I, carrying out vortex mixing on 1.0mL of precipitator II, transferring into a 100mL volumetric flask, carrying out constant volume with water to a scale, and fully and uniformly mixing; centrifuging the sample solution at 5000r/min for 10min, and collecting the supernatant II for use;
weighing 5 g-40 g of milk beverage sample, putting the sample into a conical flask, adding 20mL of warm water at 40 ℃, fully mixing, placing on an ultrasonic oscillator for ultrasonic extraction for 10min, and cooling to room temperature; adding 1.0mL of precipitator I, carrying out vortex mixing on 1.0mL of precipitator II, transferring into a 100mL volumetric flask, adding water to a constant volume to a scale, fully and uniformly mixing, centrifuging sample liquid at 5000r/min for 10min, and taking supernatant liquid for later use;
in carrying out the derivatization in the test solution:
accurately sucking 1.00mL of supernatant I, supernatant II and supernatant III into a glass test tube with a plug respectively, adding 1.00mL of sodium carbonate buffer solution and 1.00mL of dansyl chloride solution, fully mixing, carrying out room-temperature light-proof derivatization reaction for 2 hours, shaking the derivatization reaction for 1 time every 1 hour, adding 0.10mL of methylamine hydrochloride solution, carrying out vortex mixing to terminate the reaction, and standing in a light-proof manner until the precipitation is complete; filtering the supernatant fluid four by a 0.45 mu m microporous filter membrane, and taking the filtrate for later use;
taking another 1.00mL of taurine standard working solution, and synchronously derivatizing the taurine standard working solution with the test solution, wherein the steps are consistent with the steps of derivatizing;
(3) and (3) analysis results:
respectively injecting derivative solutions of the standard series working solutions into a high performance liquid chromatograph, measuring corresponding chromatographic peak height or peak area, and drawing a standard curve by taking the concentration of the standard working solutions as a horizontal coordinate and taking response values as a vertical coordinate;
the taurine content of the sample is calculated according to the following formula:
A＝C×V/m/1000×100
in the formula:
a-taurine content in milligrams per hundred grams (mg/100g) of sample;
c-concentration of taurine in the sample measurement solution in units of micrograms per milliliter
(μg/mL)；
V is the volumetric volume of the sample in milliliters (mL);
m is the mass of the sample in grams (g).
2. The method for detecting taurine in formula food for special medical application according to claim 1, wherein 1mol/L hydrochloric acid solution, 80mmol/L sodium carbonate buffer solution, 1.5mg/mL dansyl chloride solution, 20mg/L methylamine hydrochloride solution, and 10mmol/L sodium acetate buffer solution are prepared by dispensing before all steps.
3. The method for detecting taurine in formula food for special medical application according to claim 1, wherein the preparation method of the precipitating agent I comprises the following steps: weighing 15.0g of potassium ferrocyanide, dissolving with water and fixing the volume to 100 mL; the precipitant was stable for 3 months at room temperature.
4. The method for detecting taurine in formula food for special medical application according to claim 1, wherein the preparation method of the precipitant II comprises the following steps: weighing 30.0g of zinc acetate, dissolving with water and fixing the volume to 50 mL; the precipitant remained stable for 3 months at room temperature.
5. The method for detecting taurine in a formula food for special medical application according to claim 1, wherein the supernatant I, the supernatant II and the supernatant III in the step (2) are all stable within 24 hours after being stored at 4 ℃ in the dark.
6. The method for detecting taurine in formula food for special medical application according to claim 1, wherein the derivative generated during derivatization of the test solution can be stored away from light for 48 hours at a temperature of below 4 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010741833.9A CN112114061A (en) | 2020-07-29 | 2020-07-29 | Method for detecting taurine in formula food with special medical application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010741833.9A CN112114061A (en) | 2020-07-29 | 2020-07-29 | Method for detecting taurine in formula food with special medical application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112114061A true CN112114061A (en) | 2020-12-22 |
Family
ID=73799184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010741833.9A Pending CN112114061A (en) | 2020-07-29 | 2020-07-29 | Method for detecting taurine in formula food with special medical application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112114061A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114577942A (en) * | 2022-03-08 | 2022-06-03 | 山东省食品药品检验研究院 | Method for determining 22 amino acids and taurine in formula food with special medical application |
| CN114577937A (en) * | 2022-02-28 | 2022-06-03 | 上海市质量监督检验技术研究院 | Method for detecting taurine content in formula milk powder for special medical purposes |
-
2020
- 2020-07-29 CN CN202010741833.9A patent/CN112114061A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 中华人民共和国卫生部: "食品安全国家标准 婴幼儿食品和乳品中牛磺酸的测定", 《中华人民共和国国家标准 GB 5413.26-2010》 * |
| 中华人民共和国卫生部: "食品安全国家标准 食品添加剂 牛磺酸", 《中华人民共和国国家标准 GB14759—-2010》 * |
| 中华人民共和国国家卫生和计划生育委员会: "食品安全国家标准 食品中牛磺酸的测定", 《中华人民共和国国家标准GB5009.169-2016》 * |
| 刘柱 等: "酶解反应柱前衍生高效液相色谱法测定高淀粉类食品中的牛磺酸", 《理化检验-化学分册》 * |
| 陈向明: "高效液相色谱法测定牛磺酸颗粒中牛磺酸的含量", 《福建分析测试》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114577937A (en) * | 2022-02-28 | 2022-06-03 | 上海市质量监督检验技术研究院 | Method for detecting taurine content in formula milk powder for special medical purposes |
| CN114577942A (en) * | 2022-03-08 | 2022-06-03 | 山东省食品药品检验研究院 | Method for determining 22 amino acids and taurine in formula food with special medical application |
| CN114577942B (en) * | 2022-03-08 | 2024-02-23 | 山东省食品药品检验研究院 | Method for determining 22 amino acids and taurine in formula food with special medical application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112114061A (en) | Method for detecting taurine in formula food with special medical application | |
| CN108663448B (en) | Method for detecting related substances in compound amino acid injection | |
| CN101975837B (en) | Method for determining content and purity of L-carnitine in milk powder | |
| CN108693273A (en) | Aflatoxin B1, the detection method of B2, G1, G2 in a kind of Chinese medicine | |
| CN112114060A (en) | Method for detecting vitamins in formula food with special medical application | |
| CN108689933A (en) | A kind of hypochlorous fluorescence probe of quick high-selectivity analysis | |
| CN102288687A (en) | Method for analysing and detecting impurities in ornithine aspartate | |
| Street Jr et al. | Spectrofluorometric determination of acetylsalicylic acid, salicylamide, and salicylic acid as an impurity in pharmaceutical preparations | |
| CN111366649A (en) | Method for measuring 5 anions in eggs and marinated egg products by ion chromatography | |
| Hernandez et al. | Measurement of nanomolar levels of psychoactive drugs in urine by adsorptive stripping voltammetry | |
| CN111912916A (en) | Method for measuring content of index components in fingered citron preparation | |
| CN108774514A (en) | A method of detection mercury ion | |
| CN111579684B (en) | Method for measuring content of total capsaicin in capsule wall material of capsule | |
| CN112964796A (en) | Method for determining taurocholic acid in cosmetics | |
| Xie et al. | Ultrasensitive determination of amoxicillin using chemiluminescence with flow injection analysis | |
| CN109061009B (en) | Method for measuring content of itaconic acid in fermentation liquor | |
| CN107505301B (en) | Synthesis method of fluorescent probe with high fluorescence intensity and method for detecting β receptor agonist by using fluorescent probe | |
| Samanidou et al. | Validation of a novel HPLC sorbent material for the determination of ten quinolones in human and veterinary pharmaceutical formulations | |
| Sallis et al. | An assay for oxalate based on the conductometric measurement of enzyme-liberated carbon dioxide | |
| Kishimoto et al. | Method for the simplified analysis of deproteinized plasma and urinary isoproterenol by high-performance liquid chromatography | |
| CN114062558B (en) | Method for measuring sulfur dioxide in food | |
| CN111272916B (en) | Method for detecting content of penicillin V acid | |
| CN115266993A (en) | Method for detecting content of nintedanib by adopting high performance liquid chromatography | |
| SU1188604A1 (en) | Method of quantitative determination of nialamide | |
| Vinh et al. | Determination of clonazepam in tablets by dc polarography |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201222 |
|
| RJ01 | Rejection of invention patent application after publication |