CN112098655A - Heart failure detection kit based on intestinal flora - Google Patents

Heart failure detection kit based on intestinal flora Download PDF

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Publication number
CN112098655A
CN112098655A CN202010781699.5A CN202010781699A CN112098655A CN 112098655 A CN112098655 A CN 112098655A CN 202010781699 A CN202010781699 A CN 202010781699A CN 112098655 A CN112098655 A CN 112098655A
Authority
CN
China
Prior art keywords
heart failure
strip
cellulose membrane
membrane pad
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010781699.5A
Other languages
Chinese (zh)
Inventor
许顶立
曾庆春
任昊
彭健
詹琼
白煜佳
屠燕
赖文岩
黄裕立
刘祖恒
谭燕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Southern Medical University
Southern Hospital Southern Medical University
Original Assignee
Southern Medical University
Southern Hospital Southern Medical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Southern Medical University, Southern Hospital Southern Medical University filed Critical Southern Medical University
Priority to CN202010781699.5A priority Critical patent/CN112098655A/en
Publication of CN112098655A publication Critical patent/CN112098655A/en
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/32—Cardiovascular disorders
    • G01N2800/325—Heart failure or cardiac arrest, e.g. cardiomyopathy, congestive heart failure

Abstract

The invention discloses a heart failure detection kit based on intestinal flora, which comprises a shell, wherein a detection head is arranged at the front end of the shell, an installation groove is formed in the upper surface of the shell, a detection channel is formed in the upper surface of the detection head, the detection channel is communicated with the front end of the installation groove, a cellulose membrane pad is laid in the installation groove of the shell, covers are jointly arranged on the upper surfaces of the shell and the detection head, a transparent panel is filled at the rear end of each cover, a detection strip is embedded in the upper surface of the cellulose membrane pad corresponding to the rear end of the transparent panel, a quality control strip is embedded in the upper surface of the cellulose membrane pad corresponding to the front end of the transparent panel, the quality control strip is formed by a colloidal gold marking agent, and an antigen agent based on the intestinal flora is coated on the cellulose membrane pad corresponding to the front side. In the application process, whether the patient suffers from heart failure can be detected, and the method is convenient and simple to operate in the detection process and is favorable for popularization and use.

Description

Heart failure detection kit based on intestinal flora
Technical Field
The invention relates to the technical field of heart failure detection, in particular to a heart failure detection kit based on intestinal flora.
Background
Heart failure, heart failure for short, refers to a heart circulatory disturbance syndrome caused by insufficient discharge of venous return blood volume from the heart due to the failure of the systolic and/or diastolic functions of the heart, resulting in venous system blood stasis and arterial system blood perfusion deficiency, which is manifested as pulmonary congestion and vena cava congestion. Whether a patient has heart failure or not needs to be detected timely, however, the traditional detection method has the defect that the detection is troublesome and inconvenient in the process of detecting the heart failure. To this end, we propose a heart failure detection kit based on the intestinal flora.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a heart failure detection kit based on intestinal flora.
In order to achieve the purpose, the invention adopts the following technical scheme:
a heart failure detection kit based on intestinal flora comprises a shell, wherein a detection head is arranged at the front end of the shell, a mounting groove is formed in the upper surface of the shell, a detection channel is formed in the upper surface of the detection head, the front ends of the detection channel and the mounting groove are communicated, a cellulose membrane pad is laid in the mounting groove of the shell, the shell and the upper surface of the detection head are jointly provided with a cover body, the outer side of the upper surface of the shell and the outer side of the upper surface of the detection head are both provided with a connecting hole, the lower surface of the cover body is provided with a connecting pin corresponding to the connecting hole, the connecting pin is inserted into the connecting hole, the rear end of the cover body is filled with a transparent panel, a detection strip is embedded into the upper surface of the cellulose membrane pad corresponding to the rear end of the transparent panel, and a quality control strip is embedded into the upper surface, the quality control strip is composed of a colloidal gold labeling agent, and an antigen agent based on intestinal flora is coated on the cellulose membrane pad corresponding to the front side of the quality control strip.
Preferably, the detection head and the shell are of an integrated structure.
Preferably, the upper surface of the cellulose membrane mat is flush with the upper surface of the housing.
Preferably, the upper surfaces of the covers corresponding to the two ends of the detection strip are provided with first indicating head bodies, and the upper surfaces of the covers corresponding to the two ends of the quality control strip are provided with second indicating head bodies.
Preferably, the quality control strip has the same color as the background of the cellulose membrane pad.
The heart failure detection kit based on the intestinal flora has the beneficial effects that: in the using process of the detection head, the detection head is immersed in urine collected from a patient, the urine moves to the rear end of the cellulose membrane pad through capillary action, and in the moving process of the urine on the cellulose membrane pad, when the urine is in contact with the quality control strip, the urine can react with the colloidal gold labeling agent corresponding to the quality control strip, a reaction combination substance is generated between the urine and the colloidal gold labeling agent in the reaction process, and the quality control strip can develop color; the reaction combination is further specifically combined with the antigen agent of the intestinal flora corresponding to the detection strip, the reaction combination is intercepted by the detection strip and gathered on the detection strip, and the detection strip can display whether the patient corresponding to the urine suffers from the heart failure or not through color development, so that whether the patient suffers from the heart failure or not can be detected.
Drawings
FIG. 1 is a front view of a heart failure detection kit based on intestinal flora according to the present invention;
FIG. 2 is a top view of a heart failure detection kit based on intestinal flora according to the present invention;
FIG. 3 is a schematic diagram of the internal structure of a heart failure detection kit based on intestinal flora according to the present invention;
FIG. 4 is a top view of a housing of the present invention illustrating a heart failure testing kit based on intestinal flora;
fig. 5 is a bottom view of a cover of the heart failure detection kit based on intestinal flora according to the present invention.
In the figure: the device comprises a shell 1, a cover body 2, connecting pins 3, connecting holes 4, a detection head 5, a detection channel 6, a mounting groove 7, a transparent panel 8, a quality control strip 9, a detection strip 10, a first indication head body 11, a second indication head body 12 and a cellulose membrane pad 13.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-5, a heart failure detection kit based on intestinal flora comprises a shell 1, a detection head 5 is arranged at the front end of the shell 1, and the detection head 5 and the shell 1 are of an integrated structure.
Mounting groove 7 has been seted up to casing 1's upper surface, detects the upper surface of head 5 and has seted up detection channel 6, is linked together between the front end of detection channel 6 and mounting groove 7, has laid cellulose membrane pad 13 in casing 1's the mounting groove 7, and the upper surface of cellulose membrane pad 13 is parallel and level mutually with casing 1's upper surface.
Casing 1 and detection head 5's upper surface is equipped with lid 2 jointly, connecting hole 4 has all been seted up in the outside of casing 1 upper surface and the outside of detection head 5 upper surface, the lower surface of lid 2 corresponds connecting hole 4 and is equipped with connecting pin 3, connecting pin 3 inserts and establishes inside connecting hole 4, the rear end of lid 2 is filled with transparent panel 8, the upper surface embedding of the cellulose membrane pad 13 that transparent panel 8 rear end corresponds has detection strip 10, the upper surface embedding of the cellulose membrane pad 13 that transparent panel 8 front ends correspond has matter accuse strip 9, matter accuse strip 9 comprises colloidal gold marking agent, scribble on the cellulose membrane pad 13 that the front side of matter accuse strip 9 corresponds and scribble the antigenic agent based on intestinal fungus crowd.
The upper surfaces of the cover bodies 2 corresponding to the two ends of the detection strip 10 are provided with first indicating head bodies 11, the upper surfaces of the cover bodies 2 corresponding to the two ends of the quality control strip 9 are provided with second indicating head bodies 12, the background colors of the quality control strip 9 and the cellulose membrane pad 13 are the same, and the detection strip 10 and the quality control strip 9 corresponding to the quality control strip are convenient to check through the first indicating head bodies 11 and the second indicating head bodies 12.
In summary, the following steps: in the using process of the invention, the detection head 5 is immersed in urine collected from a patient, at the moment, the urine can move to the rear end of the cellulose membrane pad 13 through capillary action, and in the moving process of the urine on the cellulose membrane pad 15, when the urine is contacted with the quality control strip 9, the urine can react with the colloidal gold labeling agent corresponding to the quality control strip, a reaction conjugate is generated between the urine and the colloidal gold labeling agent in the reaction process, and the quality control strip 9 can develop color; at this time, the reaction conjugate is further specifically combined with the antigen agent of the intestinal flora corresponding to the detection strip 10, and is intercepted by the detection strip 10 and gathered on the detection strip 10, at this time, the detection strip 10 can display whether the patient corresponding to the urine suffers from the heart failure through color development, so that whether the patient suffers from the heart failure can be detected, the operation in the whole detection process is convenient and simple, namely, the detection of whether the patient suffers from the heart failure becomes simple, convenient and easy to operate, and the popularization and the use are facilitated.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (5)

1. The utility model provides a heart failure detect reagent box based on intestinal fungus crowd, includes casing (1), its characterized in that, the front end of casing (1) is equipped with detects head (5), mounting groove (7) have been seted up to the upper surface of casing (1), the upper surface of detecting head (5) has been seted up and has been detected channel (6), be linked together between the front end of detecting channel (6) and mounting groove (7), cellulose membrane pad (13) have been laid in mounting groove (7) of casing (1), the upper surface of casing (1) and detecting head (5) is equipped with lid (2) jointly, connecting hole (4) have all been seted up in the outside of casing (1) upper surface and the outside of detecting head (5) upper surface, the lower surface of lid (2) corresponds connecting hole (4) and is equipped with connecting foot (3), connecting foot (3) are inserted and are established inside connecting hole (4), transparent panel (8) are filled in the rear end of lid (2), the upper surface embedding of the cellulose membrane pad (13) that transparent panel (8) rear end corresponds has test strip (10), the upper surface embedding of the cellulose membrane pad (13) that transparent panel (8) front end corresponds has matter accuse strip (9), matter accuse strip (9) comprise colloidal gold marker, scribble on the cellulose membrane pad (13) that the front side of matter accuse strip (9) corresponds and scribble the antigen agent based on intestinal fungus crowd.
2. The enteric flora-based heart failure detection kit according to claim 1, wherein the detection head (5) and the housing (1) are integrally formed.
3. The enteric flora-based heart failure detection kit according to claim 1, wherein the upper surface of the cellulose membrane pad (13) is flush with the upper surface of the housing (1).
4. The enteric flora-based heart failure detection kit according to claim 1, wherein the first indicator heads (11) are disposed on the upper surfaces of the covers (2) corresponding to the two ends of the detection strip (10), and the second indicator heads (12) are disposed on the upper surfaces of the covers (2) corresponding to the two ends of the quality control strip (9).
5. The enteric flora-based heart failure detection kit according to claim 1, wherein the background color of the quality control strip (9) and the cellulose membrane pad (13) is the same.
CN202010781699.5A 2020-08-06 2020-08-06 Heart failure detection kit based on intestinal flora Pending CN112098655A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010781699.5A CN112098655A (en) 2020-08-06 2020-08-06 Heart failure detection kit based on intestinal flora

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010781699.5A CN112098655A (en) 2020-08-06 2020-08-06 Heart failure detection kit based on intestinal flora

Publications (1)

Publication Number Publication Date
CN112098655A true CN112098655A (en) 2020-12-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010781699.5A Pending CN112098655A (en) 2020-08-06 2020-08-06 Heart failure detection kit based on intestinal flora

Country Status (1)

Country Link
CN (1) CN112098655A (en)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753517A (en) * 1996-03-29 1998-05-19 University Of British Columbia Quantitative immunochromatographic assays
CN1191603A (en) * 1995-06-02 1998-08-26 史密丝克莱恩诊断公司 Competitive immunoassay device
US20020015947A1 (en) * 2000-06-27 2002-02-07 David Charlton Rapid methods and kits for detection and semi-quantitation of anti-adenovirus antibody
US6998273B1 (en) * 2000-02-09 2006-02-14 A-Fem Medical Corporation Collection device for lateral flow chromatography
CN101173924A (en) * 2007-11-23 2008-05-07 北京怡成生物电子技术有限公司 Biological liquid sample detecting card
US20150153366A1 (en) * 2014-02-18 2015-06-04 Melinda Sanders At-Home Blood Pregnancy Test Kit
CN107167593A (en) * 2017-06-02 2017-09-15 亳州市新健康科技有限公司 Illicit drugs inspection kit
US20190299205A1 (en) * 2018-03-31 2019-10-03 Chang Gung Memorial Hospital, Linkou Detection device
CN111020038A (en) * 2019-12-30 2020-04-17 南方医科大学南方医院 Intestinal microorganisms for diagnosing heart valve calcification, primer set and application

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1191603A (en) * 1995-06-02 1998-08-26 史密丝克莱恩诊断公司 Competitive immunoassay device
US5753517A (en) * 1996-03-29 1998-05-19 University Of British Columbia Quantitative immunochromatographic assays
US6998273B1 (en) * 2000-02-09 2006-02-14 A-Fem Medical Corporation Collection device for lateral flow chromatography
US20020015947A1 (en) * 2000-06-27 2002-02-07 David Charlton Rapid methods and kits for detection and semi-quantitation of anti-adenovirus antibody
CN101173924A (en) * 2007-11-23 2008-05-07 北京怡成生物电子技术有限公司 Biological liquid sample detecting card
US20150153366A1 (en) * 2014-02-18 2015-06-04 Melinda Sanders At-Home Blood Pregnancy Test Kit
CN107167593A (en) * 2017-06-02 2017-09-15 亳州市新健康科技有限公司 Illicit drugs inspection kit
US20190299205A1 (en) * 2018-03-31 2019-10-03 Chang Gung Memorial Hospital, Linkou Detection device
CN111020038A (en) * 2019-12-30 2020-04-17 南方医科大学南方医院 Intestinal microorganisms for diagnosing heart valve calcification, primer set and application

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Application publication date: 20201218

RJ01 Rejection of invention patent application after publication