CN112098183B - Automatic processing device and method for tissue sample dehydration embedding - Google Patents
Automatic processing device and method for tissue sample dehydration embedding Download PDFInfo
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- CN112098183B CN112098183B CN202010833406.3A CN202010833406A CN112098183B CN 112098183 B CN112098183 B CN 112098183B CN 202010833406 A CN202010833406 A CN 202010833406A CN 112098183 B CN112098183 B CN 112098183B
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- 230000018044 dehydration Effects 0.000 title claims abstract description 32
- 238000006297 dehydration reaction Methods 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 239000001993 wax Substances 0.000 claims description 26
- 239000000463 material Substances 0.000 claims description 9
- 239000012188 paraffin wax Substances 0.000 claims description 8
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 4
- 239000006247 magnetic powder Substances 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 239000000975 dye Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000004043 dyeing Methods 0.000 claims description 2
- 239000000696 magnetic material Substances 0.000 claims description 2
- 210000001519 tissue Anatomy 0.000 description 102
- 238000010586 diagram Methods 0.000 description 4
- 241000167854 Bourreria succulenta Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000019693 cherries Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229920002994 synthetic fiber Polymers 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides an automatic processing device and method for tissue sample dehydration embedding, wherein the device comprises a three-dimensional moving device, a rotary driving device, a disc collector, a multi-groove liquid exchange workbench and a tissue embedding mould box assembly; the tissue embedding die box assembly comprises an embedding die box upper cover, an embedding die box and a tissue positioning bracket positioned between the embedding die box upper cover and the embedding die box, wherein the tissue positioning bracket is used for seamlessly attaching biological tissues to the bottom of the embedding die box; the disc collector is used for accommodating at least one tissue embedding mould box assembly; the multi-tank liquid exchange workbench comprises at least one container containing melted embedded wax liquid; the three-dimensional moving device is used for driving the disc collector to move up and down in each container; the rotary driving device is used for driving the disc collector to rotate in the container. Compared with the prior art, the whole process is automatically carried out, and samples are not required to be transferred among different machines manually, so that the working efficiency is greatly improved.
Description
Technical Field
The invention relates to the field of biomedical pathological tissue automatic detection, in particular to an automatic processing device and method for tissue sample dehydration embedding.
Background
In pathological diagnosis, the work of fixing, dehydrating, embedding, slicing, labeling a sample, and the like of a biopsy is involved. It is obvious that these are cumbersome tasks. For example, the labeling of a sample, on each tissue section, requires a label to indicate what person is in what case the sample, and once labeled incorrectly, will lead to errors. As another example, conventional tissue dehydration is performed in an empty box, which is then manually positioned with wax at the bottom of the embedding box.
The American sakura test instruments society in China patent publication No. CN102292030B presents a method of positioning and securing sample tissue in a soft, microtomed box. However, this box has a wall thickness of the order of one millimeter. Thus, the paraffin-embedded tissue block is at a distance from the surface of the paraffin block, rather than being embedded as done manually, and the tissue block is on the surface of the paraffin. It is obvious that the tissue block is positioned at a deeper position of the wax block, which is inconvenient for the next slicing operation; liu Yong in CN203908850U, a method is proposed in which a piece of foam is pressed against the tissue, pushing the tissue against the walls of the embedding mould. By adopting the method, the tissue block can be pressed and fixed from one side, the periphery of the tissue is not well limited correspondingly, and the strip-shaped and sheet-shaped tissue is obviously difficult to fix to a required position. As another example, existing embedding cassettes typically have dimensions that are 5 to 10 times the volume of the tissue mass. The excessive die results in a corresponding expansion of the machine volume involved in sample processing and the amount of processing reagent, thus causing unnecessary waste.
Fixation, dehydration, paraffin embedding of tissue pieces is a complex process. The method of using manual work is complex and even with the use of automated machines today, it is necessary to manually transfer the sample between several different machines.
In summary, with the advancement of technology, although the technology of tissue fixation and embedding has been greatly improved, a complex process is still provided.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention provides an automatic processing device and method for tissue sample dehydration embedding, which are optimized in the aspects of tissue positioning embedding in an embedding block, miniaturization of an embedding mould box, sample dehydration, wax filling automation and the like, thereby realizing a machine and method capable of realizing automatic tissue embedding.
An automatic processing device for tissue sample dehydration embedding comprises a three-dimensional moving device, a rotary driving device, a disc collector, a multi-groove liquid changing workbench and a tissue embedding mould box assembly;
the tissue embedding die box assembly comprises an embedding die box upper cover, an embedding die box and a tissue positioning bracket positioned between the embedding die box upper cover and the embedding die box, wherein the tissue positioning bracket is used for seamlessly attaching biological tissues to the bottom of the embedding die box;
the disc collector is used for accommodating at least one tissue embedding mould box assembly;
the multi-tank liquid exchange workbench comprises at least one container containing melted embedded wax liquid;
the three-dimensional moving device is used for driving the disc collector to move up and down in each container;
the rotary driving device is used for driving the disc collector to rotate in the container so that the wax liquid enters the tissue embedding mould box assembly to dehydrate and embed the biological tissues in the container.
Further, the tissue positioning bracket is formed by a block-shaped compressible and rebounding penetrating porous sponge or a thorn-shaped multi-protrusion structure, and the lower part of the tissue positioning bracket is provided with a corresponding sample accommodating space; the upper part of the tissue positioning bracket is provided with a space for containing a strong magnetic material, or the tissue positioning bracket is made of a composite material consisting of plastic and nanometer magnetic powder, so that the tissue positioning bracket is adsorbed to the bottom of the embedding mould box through magnetic force.
Further, the device also comprises a support connector and a disc collector support, wherein the disc collector is arranged in the disc collector support, the disc collector support is connected with the three-dimensional moving device through the support connector, and the disc collector support is detachably connected with the support connector.
Further, the three-dimensional moving device is used for driving the disc container support through the support connector, and further driving the disc container to move in the front-back, left-right, up-down and three-axis directions, and when the multi-groove liquid changing workbench is an array formed by a plurality of containers, the disc container is driven by the three-dimensional moving device to move in each container.
Further, the upper part of the disc collector bracket is square frame-shaped, and the lower part of the disc collector bracket is semicircular arc frame-shaped, can be placed into a plurality of stacked disc collectors and allows the disc collectors to rotate in the disc collectors; the tissue embedding mould box component is divided into a standard specification and a miniature specification, and is installed in a matched mode with a standard slicing machine through a converter when the tissue embedding mould box component is of the miniature specification.
Further, the support connector is provided with an electromagnet and an alignment column which are electrically connected, the disc collector support is provided with a permanent magnet corresponding to the electromagnet, the alignment column is provided with an alignment hole, the electromagnet generates magnetic fields in different directions through electrification to magnetically attract or repel the permanent magnet, and then the alignment column is inserted into the alignment hole or separated from the alignment hole, so that the support connector and the disc collector support are connected and separated.
Further, the rotary driving device comprises a motor and a gear driven by the motor, the rotary driving device is arranged at the bottom of the bracket connector, a driving window is arranged on the top surface of the bracket of the disc container, the gear of the rotary driving device extends into the driving window from the driving window to be in driving connection with the disc container, and the disc container can be driven to rotate by starting the motor.
Further, the disc container comprises a hollow round frame, the outer wall of the hollow round frame is provided with gear teeth matched with the gear of the rotary driving device, and after the support connector is connected with the disc container support, the gear of the rotary driving device is meshed with the gear teeth of the outer wall of the hollow round frame.
Further, the surface of the tissue positioning bracket is provided with coding column holes for installing coding columns, the sections of the coding columns are all the same coding patterns, the coding columns and the sample are subjected to dehydration embedding, the coding columns and the sample are cut into slices during slicing, codes containing the sections of the coding columns are arranged beside the sample of each slice, and the coding columns are made of materials which can be dyed by tissue dyeing dyes, and the materials comprise proteins, nucleic acids, fat, sugar or other synthetic materials.
An automatic processing method for tissue sample dehydration embedding, which is carried out by adopting the device, comprises the following steps:
selecting a proper tissue embedding mould box assembly according to the size of a clinically collected fixed sample, and selecting a tissue positioning bracket with a corresponding sample accommodating space according to the shape of the sample;
step two, loading the samples and the tissue positioning brackets into corresponding embedding die boxes, adding an upper cover of the embedding die boxes to form embedding die box combinations containing the samples, fixing the embedding die box combinations containing the samples into disc collectors, and placing a plurality of the disc collectors into the disc collector brackets to obtain disc collector combinations containing the samples;
step three, arranging a disc container combination containing samples at a sample to-be-treated position on a multi-groove liquid changing workbench, moving a bracket connector to the upper part of the disc container combination by a three-dimensional moving device, and then continuing to move downwards to connect the disc container combination with the bracket connector; simultaneously, the rotary driving device drives the disc collector to enable the embedded die box combination to be positioned at a vertical position;
step four, the three-dimensional moving device drives a disc container combination containing samples to enter containers containing different solutions in sequence, and the sample dehydration process is completed;
step five, after the sample dehydration is completed, the three-dimensional moving device drives the disc container combination containing the sample to enter a container containing melted embedded wax liquid, the rotary driving device drives the disc container to rotate, so that the embedded mold box combination is in a horizontal position, the embedded mold box is filled with the wax liquid, the three-dimensional moving device moves the disc container combination up from the container embedded with the wax liquid, so that the wax liquid which cannot enter the embedded mold box is drained, then the three-dimensional moving device moves the disc container combination into a low-temperature tank, the wax liquid is cooled, then the disc container is combined to a sample collecting position, and the bracket connector is controlled to be separated from the disc container combination;
and step six, the three-dimensional moving device starts to dehydrate and embed paraffin wax for the next disc container combination.
According to the invention, through the combination of the tissue embedding mould box assembly, the disc collector, the three-dimensional moving device and the multi-groove liquid exchanging workbench, the liquid exchanging, dehydrating and embedding process is implemented through the three-dimensional movement of the three-dimensional moving device and the rotary movement of the rotary driving device driving the disc collector, and compared with the prior art, the whole process is automatically carried out, and samples are not required to be transferred among different machines manually, so that the working efficiency is greatly improved.
Drawings
FIG. 1 is a schematic diagram of one embodiment of an automated tissue sample dewatering embedding processing apparatus according to the present invention;
FIG. 2 is a schematic view of a partially exploded construction of a bracket connector, a disk collector bracket, and a disk collector according to an embodiment of the present invention;
FIG. 3 is a schematic view of a disk assembly in a horizontal position according to an embodiment of the present invention;
FIG. 4 is a schematic view of a disk assembly in a vertical position according to an embodiment of the present invention;
FIG. 5 is a schematic diagram showing a positional relationship between a disc collector and a multi-groove liquid changing table according to an embodiment of the present invention;
FIG. 6 is a schematic illustration of an exploded view of one of the tissue embedding cassettes of an embodiment of the invention;
FIG. 7 is an exploded view of another embodiment of a tissue embedding cassette of the present invention;
FIG. 8 is a schematic structural view of a tissue positioning scaffold according to an embodiment of the present invention;
FIG. 9 is a schematic diagram of an encoding column according to an embodiment of the present invention;
FIG. 10 is a schematic diagram of the structure of a sliced code and sample according to an embodiment of the present invention.
In the figure: 10-three-dimensional moving device, 20-support connector, 21-electromagnet, 22-alignment column, 30-rotation driving device, 40-disc collector, 41-hollow round frame, 42-gear tooth, 43-partition, 50-multislot liquid changing table, 60-tissue embedding mould box assembly, 61-first tissue embedding mould box, 62-second tissue embedding mould box, 70-disc collector support, 71-permanent magnet, 72-alignment hole, 73-driving window, 80-coding column, 81-coding, 90-tissue slice, 611, 621-embedding mould box upper cover, 612, 622-tissue positioning support, 613, 623-embedding mould box, 614-converter, 6121-penetrating porous sponge or thorn-shaped multi-protrusion structure, 6122-coding column hole.
Detailed Description
The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings.
Referring to fig. 1-5, an embodiment of the present invention provides an automatic processing device for tissue sample dehydration and embedding, which comprises a three-dimensional moving device 10, a rotary driving device 30, a disc collector 40, a multi-groove liquid changing table 50, and a tissue embedding module assembly 60.
The tissue embedding mould box assembly 60 comprises an embedding mould box upper cover, an embedding mould box and a tissue positioning bracket positioned between the embedding mould box upper cover and the embedding mould box, wherein the tissue positioning bracket is used for attaching biological tissues to the bottom of the embedding mould box in a seamless manner;
the disc collector 40 is adapted to receive at least one tissue embedding cassette assembly 60;
the multi-tank liquid change table 50 includes at least one container containing molten embedding wax liquid;
the three-dimensional moving device 10 is used for driving the disc collector 40 to move up and down in each container;
the rotary driving device 30 is used for driving the disc collector 40 to rotate in the container so as to enable the wax liquid to enter the tissue embedding mould box assembly 60 to carry out dehydration and paraffin embedding treatment on biological tissues in the wax liquid.
In another embodiment, the automatic tissue sample dehydration embedding treatment apparatus further comprises a bracket connector 20 and a disk container bracket 70, as shown in fig. 2, the upper part of the disk container bracket 70 is square frame-shaped, the lower part is semicircular arc frame-shaped, and a plurality of stacked disk containers 40 can be placed in the disk container bracket 70, and the disk containers 40 are installed in the disk container bracket 70 and allowed to rotate therein.
The disc container support 70 is connected with the three-dimensional moving device 10 through the support connector 20, so that the three-dimensional moving device 10 can drive the disc container support 70 through the support connector 20, further drive the disc container 40 to move in three axial directions, namely front, back, left, right, up and down, when the multi-groove liquid changing workbench 50 is an array formed by a plurality of containers, the disc container 40 can move in each container under the driving of the three-dimensional moving device 10, and both the three-dimensional moving device 10 and the rotary driving device 30 are controlled by a host machine.
The disc collector holder 70 is detachably connected to the holder connector 20, such as a clamping connection, a sleeving connection, or a magnetic connection, which is exemplified in this embodiment. The support connector 20 is provided with an electromagnet 21 and an alignment column 22 which are electrically connected, the disc collector support 70 is provided with a permanent magnet 71 corresponding to the electromagnet 21, the alignment column 22 is provided with alignment holes 72 corresponding to the alignment column 22, as shown in fig. 2, the alignment column 22 is arranged at four corners of the bottom surface of the support connector 20, and four corners of the top surface of the disc collector support 70 are provided with alignment holes 72 corresponding to the alignment column 22. When the three-dimensional moving device is in operation, the electromagnet 21 is electrified to magnetically attract the permanent magnet 71, and the alignment post 22 of the bracket connector 20 is correspondingly inserted into the alignment hole 72 of the disk collector bracket 70, so that the bracket connector 20 is connected with the disk collector bracket 70, and the three-dimensional moving device 10 is started to move the position of the disk collector 40; when no movement is required, the electromagnet 21 may be energized to generate a magnetic field opposite to the permanent magnet 71 on the disk cartridge holder 70, thereby separating the holder connector 20 from the disk cartridge holder 70.
The rotation driving device 30 may include a motor and a gear driven by the motor, the rotation driving device 30 is mounted at the bottom of the bracket connector 20, a driving window 73 is provided on the top surface of the disc collector bracket 70, the gear of the rotation driving device 30 extends into the disc collector 40 from the driving window 73 to be in driving connection with the disc collector 40, and the motor can be started to drive the disc collector 40 to rotate.
The disc collector 40 comprises a hollow circular frame 41, gear teeth 42 matched with the gears of the rotary driving device 30 are arranged on the outer wall of the hollow circular frame 41, and a plurality of partition plates are connected in the hollow circular frame 41 to form a separation cavity 43 for accommodating the tissue embedding mould box assembly 60. When in operation, after the bracket connector 20 is connected with the disk container bracket 70, the gear of the rotary driving device 30 is meshed with the gear teeth 42 on the outer wall of the hollow round frame 41, and then the motor is started to drive the gear to rotate, so that the hollow round frame 41 of the disk container 40 is driven to rotate to a horizontal position (shown in fig. 3) or a vertical position (shown in fig. 4).
With continued reference to fig. 6 and 7, the tissue embedding cassette assembly 60 of the present invention has two specifications, a first tissue embedding cassette 61 and a second tissue embedding cassette 62. Wherein the first tissue embedding mold 61 is of a miniature size and the second tissue embedding mold 62 is of a standard size.
The first tissue embedding die 61 comprises an embedding die upper cover 611, a tissue positioning bracket 612 and an embedding die 613, wherein the tissue positioning bracket 612 is positioned between the embedding die upper cover 611 and the embedding die 613, and the shape of the tissue positioning bracket 612 is consistent with the tissue embedding space formed by the embedding die upper cover 611 and the embedding die 613. The tissue positioning support 612 is composed of a block-shaped compressible and rebounding penetrating porous sponge or thorn-shaped multi-protrusion structure 6121, and the surface of the tissue positioning support 612 is provided with coding column holes 6122 for installing coding columns 80 (as shown in fig. 9)
The tissue positioning support 612 has a space corresponding to the shape of the embedded biological tissue sample at the center of the bottom of the contact embedding cassette 613. In practical use, the sample size may be slightly larger than the space. Thus, immediately after tissue placement, the sample will be compressed by slightly squeezing the tissue positioning scaffold 612. After the subsequent dewatering treatment, the sample will shrink to conform to the space after rebound. It will be apparent that the tissue positioning scaffold 612 of the present invention has the advantage of orienting and holding samples in the box of the us cherry blossom company, but allows the samples to be applied directly to the bottom of the box rather than having a millimeter gap as in the box of the us cherry company.
The first tissue embedding cassette 61 may also include a transducer 614, through which transducer 614 may be mated with a standard microtome. This has the advantage that the machine volume for processing the sample can be greatly reduced, while various reagents are saved.
The second tissue embedding cassette 62 shown in fig. 7 is similar to the first tissue embedding cassette 61 in structure and comprises an embedding cassette upper cover 621, a tissue positioning support 622 and an embedding cassette 623, wherein the difference is only that the size is slightly larger, a cover plate capable of closing the opening is arranged at the edge of the upper opening of the embedding cassette 623, and a converter matched with a standard slicing machine is not required.
As shown in fig. 9 and 10, the present embodiment adopts an intra-slice coding technique, and the principle thereof is as follows: a coding column 80 made of a material (such as protein, nucleic acid, fat, sugar, and the like, and other synthetic materials) that can be cut into pieces and stained with tissue staining dye is manufactured, the cross sections of the coding columns 80 are all the same coding patterns, the patterns can be bar codes, two-dimensional codes, characters, and the like, and the coding patterns of each coding column are unique. In use, the code column 80 is attached to a sample, and as shown in FIG. 8, the code column 80 is loaded into the code column bore 6122 of the tissue positioning scaffold 612, such that the code column 80 is subjected to dehydration embedding along with the sample. At the time of slicing, the code column 80 is sliced together with the sample. The sliced code 81 is subjected to a subsequent staining process along with the tissue slice 90. As such, each slice finally has a unique code 81 of the code column cross-section next to the sample.
An example of implementation is provided below with respect to the fabrication of a two-dimensional encoding column: by the principle similar to a three-dimensional printer, muscle tissue materials and nucleic acid materials formed by proteins are respectively stacked into long bar columns according to different thicknesses in sequence, so that the section of the coding column 80 has a coding rule of two-dimensional codes. The encoded column 80 is processed along with the sample and the final section may be stained with hematoxylin-eosin. In the encoding column 80, the protein-composed muscle tissue material will be stained pink, while the nucleic acid material will be stained dark purple. All information of the tissue slice 90 is available through the optical lens and computer automated identification.
Further, the upper part of the tissue positioning support (612, 622) can be of any shape under the condition that the lower part of the tissue positioning support has a corresponding sample accommodating space, and the tissue positioning support is fixed to the bottom of the embedding mould boxes (613, 623) through strong magnetic rings or strong magnetic columns.
Further, the tissue positioning brackets (612, 622) can be made of a composite material consisting of plastic and nano magnetic powder, so that samples with any shape can be directly adsorbed to the bottom of the embedding mould boxes (613, 623) through the reserved space by magnetic force.
The embodiment of the invention also provides an automatic processing method for tissue sample dehydration embedding, which is carried out by adopting the device, and comprises the following steps:
step one, according to the size of the clinically collected fixed sample, a suitable tissue embedding cassette assembly 60, such as a first tissue embedding cassette 61 or a second tissue embedding cassette 62, is selected, and then, according to the shape of the sample, tissue positioning scaffolds (612, 622) with corresponding sample receiving spaces are selected. As already mentioned above, in order to avoid the position change caused by the reduced shape shrinkage of the sample after dehydration, the tissue positioning scaffold should be selected according to the actual situation. Namely: the sample receiving space in the tissue positioning scaffold should be slightly smaller than the sample that has not yet contracted. Of course, the strong magnetic ring or the strong magnetic column fixing bracket can be selected, and the tissue embedding bracket made of the composite material consisting of the plastic and the nanometer magnetic powder can also be selected.
Step two, loading the sample and tissue positioning brackets (612, 622) into the corresponding embedding cassettes (613, 623) and adding the embedding cassette upper covers (611, 621), thus obtaining an embedding cassette combination containing the sample, fixing the embedding cassette combination containing the sample to the corresponding compartment 43 in the disk collector 40, placing a plurality of such disk collectors 40 into the disk collector brackets 70, obtaining a disk collector combination containing the sample;
step three, placing the disc container combination containing the samples at a sample to be processed position on the multi-groove liquid changing workbench 50, starting a host, moving the bracket connector 20 fixed on the three-dimensional moving device 10 to the upper side of the disc container combination by the host, and then continuing to move downwards to connect the disc container combination and the bracket connector 20 together; simultaneously, the rotary driving device 30 is inserted into the driving window 73 on the disc collector bracket 70, and the rotary driving device 30 drives the disc collector 40 to enable the embedding mould box combination to be positioned in the vertical position;
step four, the three-dimensional moving device 10 drives a disc container combination containing samples to enter containers containing different solutions in sequence, so that a sample dehydration process is completed;
step five, after the sample dehydration is completed, the three-dimensional moving device 10 drives the disc container combination containing the sample to enter a container containing melted embedded wax liquid, the rotary driving device 30 drives the disc container 40 to rotate, so that the embedded mold box combination is in a horizontal position to enable the wax liquid to enter, the embedded mold boxes (613 and 623) are filled, the three-dimensional moving device 10 moves the disc container combination up from the container embedded with the wax liquid to drain the wax liquid which cannot enter the embedded mold box, then the three-dimensional moving device 10 moves the disc container combination into a low-temperature tank, the wax liquid is cooled, and then the disc container combination is assembled to a sample collecting place; under the control of the host, the electromagnet on the bracket connector 20 is started to separate the bracket connector 20 from the disc container combination;
step six, the three-dimensional moving device 10 starts to dewater and paraffin-embedding the next disc container combination. Of course, the disk collectors may be mixed and interleaved in combination according to circumstances.
Finally, the paraffin-embedded tissue sample is removed from the disk assembly 40 by the user and placed on a universal microtome for sectioning. The sample paraffin block in the mini-embedding cassette may be mounted with a converter 614 and mounted on a universal microtome for sectioning.
Preferably, in step two, the sample and the tissue positioning support (612, 622) are loaded into the corresponding embedding cassettes (613, 623), and the code column 80 is mounted on the tissue positioning support 612, so that the code column 80 and the sample are subjected to dehydration embedding, and the code column 80 and the sample are sliced together in the subsequent slicing process, and the code containing the code column section is arranged beside each sliced sample.
The invention realizes the liquid-changing and dewatering embedding process by the combination of the tissue embedding mould box assembly 60, the disc container 40, the three-dimensional moving device 10 and the multi-groove liquid-changing workbench 50 and the three-dimensional movement and the rotary movement of the disc container 40 driven by the rotary driving device 30, and the whole process is automatically carried out, thus improving the working efficiency compared with the prior art.
The foregoing is merely illustrative embodiments of the present invention, and the present invention is not limited thereto, and any changes or substitutions that may be easily contemplated by those skilled in the art within the scope of the present invention should be included in the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.
Claims (7)
1. An automatic processing device for tissue sample dehydration embedding, which is characterized in that: comprises a three-dimensional moving device, a rotary driving device, a disc collector, a multi-groove liquid changing workbench and a tissue embedding mould box assembly;
the tissue embedding die box assembly comprises an embedding die box upper cover, an embedding die box and a tissue positioning bracket positioned between the embedding die box upper cover and the embedding die box, wherein the tissue positioning bracket is used for seamlessly attaching biological tissues to the bottom of the embedding die box;
the disc collector is used for accommodating at least one tissue embedding mould box assembly;
the multi-tank liquid exchange workbench comprises at least one container containing melted embedded wax liquid;
the three-dimensional moving device is used for driving the disc collector to move up and down in each container;
the rotary driving device is used for driving the disc collector to rotate in the container so as to enable the wax liquid to enter the tissue embedding mould box assembly and carry out dehydration and paraffin embedding treatment on biological tissues in the wax liquid;
the device comprises a three-dimensional moving device, a disc container and a support connector, wherein the disc container is arranged in the disc container support, the disc container support is connected with the three-dimensional moving device through the support connector, and the disc container support is detachably connected with the support connector;
the rotary driving device comprises a motor and a gear driven by the motor, the rotary driving device is arranged at the bottom of the bracket connector, a driving window is arranged on the top surface of the bracket of the disc container, the gear of the rotary driving device extends into the driving window from the driving window to be in driving connection with the disc container, and the disc container can be driven to rotate by starting the motor;
the disc container comprises a hollow circular frame, gear teeth matched with the gear of the rotary driving device are arranged on the outer wall of the hollow circular frame, and the gear of the rotary driving device is meshed with the gear teeth of the outer wall of the hollow circular frame after the support connector is connected with the disc container support.
2. The automated tissue sample dehydration embedding processing apparatus of claim 1, wherein: the tissue positioning bracket is composed of a block-shaped compressible and rebounding penetrating porous sponge or a thorn-shaped multi-protrusion structure, and a corresponding sample accommodating space is arranged at the lower part of the tissue positioning bracket; the upper part of the tissue positioning bracket is provided with a space for containing a strong magnetic material, or the tissue positioning bracket is made of a composite material consisting of plastic and nanometer magnetic powder, so that the tissue positioning bracket is adsorbed to the bottom of the embedding mould box through magnetic force.
3. The automated tissue sample dehydration embedding processing apparatus of claim 1, wherein: the three-dimensional moving device is used for driving the disc container support through the support connector, further driving the disc container to move in the front-back, left-right, up-down and three-axis directions, and when the multi-groove liquid changing workbench is an array formed by a plurality of containers, the disc container is driven by the three-dimensional moving device to move in each container.
4. The automated tissue sample dehydration embedding processing apparatus of claim 1, wherein: the upper part of the disc collector bracket is square frame-shaped, the lower part of the disc collector bracket is semicircular arc frame-shaped, a plurality of stacked disc collectors can be placed in the disc collector bracket, and the disc collectors are allowed to rotate in the disc collectors; the tissue embedding mould box component is divided into a standard specification and a miniature specification, and is installed in a matched mode with a standard slicing machine through a converter when the tissue embedding mould box component is of the miniature specification.
5. The automated tissue sample dehydration embedding processing apparatus of claim 4, wherein: the support connector is provided with an electromagnet and an alignment column which are electrically connected, the disc collector support is provided with a permanent magnet corresponding to the electromagnet, the alignment column is provided with an alignment hole, the electromagnet generates magnetic fields in different directions through electrification to magnetically attract or repel the permanent magnet, and the alignment column is inserted into the alignment hole or separated from the alignment hole, so that the support connector and the disc collector support are connected and separated.
6. The automated tissue sample dehydration embedding processing apparatus of claim 1, wherein: the surface of the tissue positioning bracket is provided with coding column holes for installing coding columns, the sections of the coding columns are all the same coding patterns, the coding columns and the sample are subjected to dehydration embedding, the coding columns and the sample are cut into slices during slicing, codes containing the sections of the coding columns are arranged beside the sample of each slice, the coding columns are made of materials which can be dyed by tissue dyeing dyes, and the materials comprise protein, nucleic acid, fat or sugar.
7. A method for automatically processing a dehydrated embedding of a tissue sample, characterized in that it is carried out by using the device according to any one of claims 1 to 6, said method comprising the steps of:
selecting a proper tissue embedding mould box assembly according to the size of a clinically collected fixed sample, and selecting a tissue positioning bracket with a corresponding sample accommodating space according to the shape of the sample;
step two, loading the samples and the tissue positioning brackets into corresponding embedding die boxes, adding an upper cover of the embedding die boxes to form embedding die box combinations containing the samples, fixing the embedding die box combinations containing the samples into disc collectors, and placing a plurality of the disc collectors into the disc collector brackets to obtain disc collector combinations containing the samples;
step three, arranging a disc container combination containing samples at a sample to-be-treated position on a multi-groove liquid changing workbench, moving a bracket connector to the upper part of the disc container combination by a three-dimensional moving device, and then continuing to move downwards to connect the disc container combination with the bracket connector; simultaneously, the rotary driving device drives the disc collector to enable the embedded die box combination to be positioned at a vertical position;
step four, the three-dimensional moving device drives a disc container combination containing samples to enter containers containing different solutions in sequence, and the sample dehydration process is completed;
step five, after the sample dehydration is completed, the three-dimensional moving device drives the disc container combination containing the sample to enter a container containing melted embedded wax liquid, the rotary driving device drives the disc container to rotate, so that the embedded mold box combination is in a horizontal position, the embedded mold box is filled with the wax liquid, the three-dimensional moving device moves the disc container combination up from the container embedded with the wax liquid, so that the wax liquid which cannot enter the embedded mold box is drained, then the three-dimensional moving device moves the disc container combination into a low-temperature tank, the wax liquid is cooled, then the disc container is combined to a sample collecting position, and the bracket connector is controlled to be separated from the disc container combination;
and step six, the three-dimensional moving device starts to dehydrate and embed paraffin wax for the next disc container combination.
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