CN112094734A - High-throughput gene sequencing device for cancer cell gene mutation research - Google Patents
High-throughput gene sequencing device for cancer cell gene mutation research Download PDFInfo
- Publication number
- CN112094734A CN112094734A CN201910528269.XA CN201910528269A CN112094734A CN 112094734 A CN112094734 A CN 112094734A CN 201910528269 A CN201910528269 A CN 201910528269A CN 112094734 A CN112094734 A CN 112094734A
- Authority
- CN
- China
- Prior art keywords
- sequencing
- cell
- cancer cell
- gene
- self
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a high-throughput gene sequencing device for cancer cell gene mutation research, which comprises a support pedestal, a cell sequencing driving structure, a sequencing self-cleaning structure, a self-cleaning liquid collecting structure and a sequencing chamber drying structure, wherein a connecting hinge is arranged at the left upper side of the support pedestal, the upper part of the support pedestal is hinged with a cover plate through the connecting hinge, a handle is arranged on the right end surface of the cover plate, a movable cavity is arranged in the cover plate, and the cell sequencing driving structure is arranged in the movable cavity. The cell sequencing driving structure can provide power for the circulation measurement of cancer cells conveniently, the stepping motor can enable the cancer cell tissue fluid to stay above the sequencing structure for a short time, the cancer cell tissue fluid can flow into the sequencing structure conveniently, and the gene sequencing chip arranged in the cell sequencing chamber can complete the measurement of cancer cell genes.
Description
Technical Field
The invention relates to the technical field of cancer cell gene mutation research devices, in particular to a high-throughput gene sequencing device for cancer cell gene mutation research.
Background
Gene sequencing is a novel gene detection technology, can analyze and determine the complete sequence of genes from blood or saliva, and predicts the possibility of suffering from various diseases, and the behavior characteristics and behaviors of individuals are reasonable. The gene sequencing technology can lock the individual pathological change gene and prevent and treat in advance. Products and techniques related to gene sequencing have evolved from laboratory studies to clinical use, and gene sequencing technology can be said to be the next world-changing technology. Gene sequencing is only one of gene detection methods, which is called gene spectrum sequencing, and is an internationally recognized gene detection standard. The gene sequencing technology is developed to the present, the corresponding gene sequencer also undergoes the change of covering the ground, the flux, the sequencing accuracy, the sequencing period, the reading length and the like are continuously promoted, the application is more and more extensive, and the most key part of the gene sequencing is mainly completed by a gene sequencing chip. In recent years, many studies on gene sequencing have been conducted around the development of gene chips, and remarkable achievements and developments have been obtained.
The traditional gene sequencing device cannot perform circulating detection on cells when in use, and residual cancer cells exist during detection, so that the detection result is easily influenced, and the proper survival temperature cannot be provided when the cancer cells are stored.
Disclosure of Invention
The invention aims to provide a high-throughput gene sequencing device for cancer cell gene mutation research, and solves the problems that the conventional gene sequencing device cannot perform circulating detection on cells during use, residual cancer cells exist during detection, the detection result is easily influenced, and a proper survival temperature cannot be provided during storage of the cancer cells.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a high flux gene sequencing device for research of cancer cell gene mutation, collects structure and sequencing room stoving structure including support pedestal, cell sequencing drive structure, sequencing automatically cleaning structure, automatically cleaning liquid, the upper left side of supporting the pedestal is installed and is connected the hinge, and the top of supporting the pedestal is through connecting hinge and apron between articulated, the handle is installed to the right-hand member face of apron, and the inside of apron is provided with movable chamber, cell sequencing drive structure installs in movable chamber's inside.
Preferably, cell sequencing drive structure includes step motor, shaft coupling, drive shaft and bears the dish, and step motor's output is connected with the shaft coupling, the output of shaft coupling is connected with the drive shaft, and the top of drive shaft is installed and is born the dish to step motor passes through the shaft coupling and drive shaft and bears and constitute revolution mechanic between the dish.
Preferably, bear the weight of the inside of dish and wait the angle and be provided with sequencing cell and store the structure, and sequencing cell stores the structure and covers diaphragm, heating wire, rotation stirring rod, loop bar, spout and cell storage cylinder including pasting, the upper end of cell storage cylinder is connected with pastes and covers the diaphragm, and pastes the outside of covering the diaphragm and seted up the spout to it has the heating wire to inlay in the inner wall of cell storage cylinder, the inside of cell storage cylinder is provided with the loop bar, and the outside of loop bar has cup jointed the rotation and has stirred the pole.
Preferably, the number of the sequencing cell storage structures is six, the pasting membrane and the upper end face of the cell storage cylinder are connected in an adhesive mode, and a rotating structure is formed between the rotating stirring rod and the sleeve rod.
Preferably, the sequencing self-cleaning structure is arranged at the lower right end of the support pedestal, the sequencing self-cleaning structure comprises a cleaning liquid tank, a micro hydraulic pump, a first sprinkler head and a second sprinkler head, the micro hydraulic pump is arranged at the top end inside the cleaning liquid tank, the upper end of the micro hydraulic pump is connected with the first sprinkler head through a pipeline, and the left side of the micro hydraulic pump is connected with the second sprinkler head through a pipeline.
Preferably, the cleaning liquid tank is communicated with the first sprinkler head through a micro hydraulic pump and a pipeline, the cleaning liquid tank is communicated with the second sprinkler head through a micro hydraulic pump and a pipeline, and the first sprinkler head and the cell storage cylinder are perpendicular to each other.
Preferably, bear the weight of the below of dish and be provided with cell sequencing room, and the internally mounted of cell sequencing room has the slide bar, gene sequencing chip is installed to the below of slide bar, and linear connection between gene sequencing chip's the below through wire and the electrical control box, miniature electric putter is installed in cell sequencing room's left side, and miniature electric putter's top is connected with the baffle to constitute extending structure between miniature electric putter and the baffle.
Preferably, the self-cleaning liquid collecting structure is arranged at the lower left end of the support pedestal and comprises a collecting box connecting frame, a collecting box and a communicating hole, the collecting box is arranged in the self-cleaning liquid collecting structure, the communicating hole penetrates through the tail end of the collecting box, and the collecting box connecting frame is movably connected with the collecting box.
Preferably, sequencing room stoving structure sets up in the upper left end of cell sequencing room, and sequencing room stoving structure includes the pump body, intercommunication pipeline and heater strip, the right lower extreme of the pump body is connected with the intercommunication pipeline, and the internally mounted of intercommunication pipeline has the heater strip to the pump body links to each other between through intercommunication pipeline and the cell sequencing room and communicates with each other.
Compared with the prior art, the invention has the following beneficial effects:
1. the cell sequencing driving structure can provide power for the circulation measurement of the cancer cells conveniently, so that the cancer cells can have sufficient power during sequencing, and the step motor can enable the cancer cell tissue fluid to stay above the sequencing structure for a short time, so that the cancer cell tissue fluid can flow into the sequencing structure conveniently and smoothly.
2. The sequencing cell storage structure provided by the invention can be used for conveniently and temporarily storing the cancer cell tissue fluid, a good living environment can be provided for the cancer cell tissue fluid during storage, the temperature in the cell storage cylinder can be increased through the electric heating wire, the cancer cell tissue fluid is in a proper temperature environment, the tissue fluid can be fully stirred through the arrangement of the rotary stirring rod, and cells in the tissue fluid are uniformly distributed.
3. The sequencing self-cleaning structure can conveniently clean residual liquid after detection of cancer cell tissue fluid, so that influence on next sequencing of the cancer cell tissue fluid is avoided, and the sequencing self-cleaning structure can clean the cell sequencing chamber and the cell storage cylinder through the first sprinkler head and the second sprinkler head arranged in the sequencing self-cleaning structure, so that the cleanness of the cell sequencing chamber and the cell storage cylinder is ensured.
4. The gene sequencing chip arranged in the cell sequencing chamber can complete the measurement of cancer cell genes, and meanwhile, the opening and closing of the cell sequencing chamber can be controlled by controlling the expansion and contraction of the baffle plate through the miniature electric push rod, so that the waste liquid after measurement can be conveniently treated.
5. According to the invention, the collection of the waste liquid after measurement can be completed through the arranged self-cleaning liquid collection structure, so that the problem of environmental pollution caused by poor treatment of the waste liquid generated by the device can be avoided, the drying process in the drying structure of the sequencing chamber can be completed through the arrangement of the drying structure of the sequencing chamber, the problem of corrosion of internal parts of the device caused by long-time non-use is avoided, and the practicability of the high-throughput gene sequencing device for cancer cell gene mutation research is greatly improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of a cell sequencing driver according to the present invention;
FIG. 3 is a schematic view of the interior of the self-cleaning liquid collection structure of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 1A;
FIG. 5 is an enlarged view of a portion B of FIG. 1 according to the present invention.
In the figure: 1. a support pedestal; 2. a cover plate; 3. a connecting hinge; 4. a handle; 5. a movable cavity; 6. a cell sequencing drive architecture; 601. a stepping motor; 602. a coupling; 603. a drive shaft; 604. a carrier tray; 7. sequencing the cell storage structure; 701. pasting a membrane; 702. an electric heating wire; 703. rotating the stirring rod; 704. a loop bar; 705. a chute; 706. a cell storage jar; 8. sequencing the self-cleaning structure; 801. a cleaning liquid tank; 802. a micro hydraulic pump; 803. a first sprinkler head; 804. a second spray head; 9. a cell sequencing chamber; 10. gene sequencing chip; 11. a slide bar; 12. a self-cleaning liquid collection structure; 121. the collecting box is connected with the frame; 122. a collection box; 123. a communicating hole; 13. a sequencing room drying structure; 131. a pump body; 132. a communicating pipe; 133. heating wires; 14. an electric push rod; 15. a baffle plate; 16. an electrical control box.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, a high throughput gene sequencing device for cancer cell gene mutation research comprises a support pedestal 1, a cover plate 2, a connection hinge 3, a handle 4, a movable chamber 5, a cell sequencing driving structure 6, a stepping motor 601, a coupling 602, a driving shaft 603, a bearing plate 604, a sequencing cell storage structure 7, a coating film 701, a heating wire 702, a rotating stirring rod 703, a sleeve rod 704, a chute, a cell storage cylinder 706, a sequencing self-cleaning structure 8, a cleaning solution tank 801, a micro hydraulic pump 802, a first spray head 803, a second spray head 804, a cell sequencing chamber 9, a gene sequencing chip 10, a sliding rod 11, a self-cleaning solution collection structure 12, a collection box connection frame 121, a collection box 122, a communication hole 123, a sequencing chamber drying structure 13, a pump body 131, a communication pipe 132, a heating wire 133, a micro electric push rod 14, a baffle 15, An electric control box 16, a connecting hinge 3 is installed on the upper left side of a support pedestal 1, the upper side of the support pedestal 1 is hinged with a cover plate 2 through the connecting hinge 3, a handle 4 is installed on the right end face of the cover plate 2, a movable cavity 5 is arranged inside the cover plate 2, a cell sequencing driving structure 6 is installed inside the movable cavity 5, the cell sequencing driving structure 6 comprises a stepping motor 601, a coupler 602, a driving shaft 603 and a bearing plate 604, the output end of the stepping motor 601 is connected with the coupler 602, the output end of the coupler 602 is connected with the driving shaft 603, the bearing plate 604 is installed at the top end of the driving shaft 603, the stepping motor 601 forms a rotating structure through the coupler 602 and the space between the driving shaft 603 and the bearing plate 604, power can be conveniently provided for the circulation measurement of cancer cells through the arranged cell sequencing driving structure 6, so that the cancer cells can have sufficient power during sequencing, and the arrangement of the stepping motor 601 can enable the cancer cell tissue fluid to temporarily stay above the sequencing structure, thereby facilitating the cancer cell tissue fluid to smoothly flow into the sequencing structure,
the sequencing cell storage structure 7 is arranged in the bearing disc 604 at equal angles, the sequencing cell storage structure 7 comprises a pasting membrane 701, a heating wire 702, a rotating stirring rod 703, a loop bar 704, a sliding groove 705 and a cell storage cylinder 706, the upper end of the cell storage cylinder 706 is connected with the pasting membrane 701, the outer side of the pasting membrane 701 is provided with the sliding groove 705, the inner wall of the cell storage cylinder 706 is embedded with the heating wire 702, the loop bar 704 is arranged in the cell storage cylinder 706, the outer side of the loop bar 704 is sleeved with the rotating stirring rod 703, six groups of sequencing cell storage structures 7 are arranged, the connection mode between the pasting membrane 701 and the upper end face of the cell storage cylinder 706 is adhesive connection, the rotating structure is formed between the rotating stirring rod 703 and the loop bar 704, the sequencing cell storage structure 7 can conveniently store cancer cell tissue fluid for a short time, and can provide a good living environment for the cancer cell tissue fluid during storage, the temperature inside the cell storage cylinder 706 can be raised by the electric heating wire 702, so that the cancer cell tissue fluid is in a proper temperature environment, and the tissue fluid can be fully stirred by rotating the stirring rod 703, so that the cells in the tissue fluid are uniformly distributed,
the sequencing self-cleaning structure 8 is arranged at the right lower end of the support pedestal 1, the sequencing self-cleaning structure 8 comprises a cleaning liquid tank 801, a micro hydraulic pump 802, a first sprinkler head 803 and a second sprinkler head 804, the micro hydraulic pump 802 is arranged at the top end inside the cleaning liquid tank 801, the upper end of the micro hydraulic pump 802 is connected with the first sprinkler head 803 through a pipeline, the left side of the micro hydraulic pump 802 is connected with the second sprinkler head 804 through a pipeline, the cleaning liquid tank 801 is connected and communicated with the first sprinkler head 803 through the micro hydraulic pump 802 and the pipeline, the cleaning liquid tank 801 is connected and communicated with the second sprinkler head 804 through the micro hydraulic pump 802 and the pipeline, the first sprinkler head 803 and the cell storage cylinder 706 are perpendicular to each other, and the sequencing self-cleaning structure 8 is arranged to facilitate cleaning of residual liquid after detection of cancer cell tissue liquid, so as to avoid affecting sequencing of next cancer cell tissue liquid, and the sequencing self-cleaning structure 8 can complete the cleaning of the two parts of the cell sequencing chamber 9 and the cell storage cylinder 706 through the first sprinkler head 803 and the second sprinkler head 804 arranged inside, thereby ensuring the cleanness of the inside of the cell sequencing chamber 9 and the cell storage cylinder 706,
a cell sequencing chamber 9 is arranged below the bearing disc 604, a slide bar 11 is arranged in the cell sequencing chamber 9, a gene sequencing chip 10 is arranged below the slide bar 11, the lower part of the gene sequencing chip 10 is linearly connected with an electrical control box 16 through a lead, a micro electric push rod 14 is arranged on the left side of the cell sequencing chamber 9, the top end of the micro electric push rod 14 is connected with a baffle plate 15, a telescopic structure is formed between the micro electric push rod 14 and the baffle plate 15, the gene sequencing chip 10 arranged in the cell sequencing chamber 9 can complete measurement of cancer cell genes, and the control of opening and closing of the cell sequencing chamber 9 can be completed through the telescopic control of the baffle plate 15 by the micro electric push rod 14, so that waste liquid after measurement can be conveniently treated;
the self-cleaning liquid collecting structure 12 is arranged at the left lower end of the supporting pedestal 1, the self-cleaning liquid collecting structure 12 comprises a collecting box connecting frame 121, a collecting box 122 and a communicating hole 123, the collecting box 122 is arranged in the self-cleaning liquid collecting structure 12, the communicating hole 123 penetrates through the tail end of the collecting box 122, the collecting box connecting frame 121 is movably connected with the collecting box 122, the sequencing chamber drying structure 13 is arranged at the left upper end of the cell sequencing chamber 9, the sequencing chamber drying structure 13 comprises a pump body 131, a communicating pipeline 132 and a heating wire 133, the communicating pipeline 132 is connected with the right lower end of the pump body 131, the heating wire 133 is arranged in the communicating pipeline 132, the pump body 131 is communicated with the cell sequencing chamber 9 through the communicating pipeline 132, the self-cleaning liquid collecting structure 12 is arranged to collect the measured waste liquid, and therefore the problem of environmental pollution caused by poor treatment of the waste liquid generated by the device can be avoided, and the setting of sequencing room stoving structure 13 can accomplish the process of drying in sequencing room stoving structure 13, avoids the device need not cause the problem of inside spare part corrosion for a long time, has improved the practicality of this kind of cancer cell gene mutation research's high flux gene sequencing device greatly.
The working principle is as follows: for the gene sequencing device, firstly, the cover plate 2 can play a role of covering and protecting the internal components of the device, the cover plate 2 can be driven to move by pulling the handle 4, the cover plate 2 can be rotated and opened from the support pedestal 1 through the connecting hinge 3, cancer cell tissues to be detected are led into the cell storage cylinder 706 of the sequencing cell storage structure 7, the arranged heating wire 702 can heat the inside of the cell storage cylinder 706, so that an environment suitable for the survival of cancer cells is ensured to be arranged in the cell storage cylinder 706, after the cancer cell tissue liquid is led into the cell storage cylinder 706, the cell storage cylinder 706 is sealed by the pasting membrane 701, the arranged electric control box 16 can be communicated with an external power supply and can supply power for the electric device in the device, the operation of the device can be controlled through the internal control switch, and the cell sequencing driving structure 6 is started at the same time, the driving shaft 603 can be driven to rotate by the operation of the stepping motor 601, the driving shaft 603 is connected with the output end of the stepping motor 601 through the coupling 602, and the bearing disc 604 is arranged at the top end of the driving shaft 603, so that the bearing disc 604 can be driven to rotate in the movable cavity 5, the cell tissue fluid can rock in the cell storage cylinder 706 by the rotation of the bearing disc 604, so that the rotating stirring rod 703 can be driven to move, the rotating stirring rod 703 is movably connected with the sleeve rod 704, so that the rotating stirring rod 703 can rotate, and the rotating stirring rod 703 can slightly stir the cancer cell tissue fluid,
when the bearing disc 604 rotates, the sliding rod 11 slides in the sliding groove 705 on the circumference of the bearing disc 604, and when the sliding rod 11 slides to the cylinder opening of the cell storage cylinder 706, the sliding rod 11 will cut the covering membrane 701, so that the cancer cell tissue fluid in the cell storage cylinder 706 flows into the cell sequencing chamber 9 along with the sliding rod 11, and the gene sequencing chip 10 in the cell sequencing chamber 9 can complete sequencing of the cancer cell, the upper end surface of the gene sequencing chip 10 is in contact with the cancer cell tissue fluid, after contacting with the cancer cell tissue fluid sample reagent, the number and sequence information of the sample molecules can be obtained, after the cell sequencing, the electric push rod 14 is started, the electric push rod 14 can work to drive the baffle 15 to extend and retract up, when the baffle 15 rises, the left side wall of the cell sequencing chamber 9 can be opened, so that the cancer cell tissue fluid can flow out, the sequencing self-cleaning structure 8 is started, the micro hydraulic pump 802 is electrified to work to draw out the cleaning fluid in the cleaning fluid tank 801, and is transported to the first sprinkler 803 and the second sprinkler 804 through pipelines, the first sprinkler 803 is perpendicular to the cell storage cylinder 706, so that the cell storage cylinder 706 can be cleaned, and the liquid generated by the cleaning can flow into the self-cleaning liquid collecting structure 12, and the second sprinkler 804 can clean the cell sequencing chamber 9, so that the upper end surface of the gene sequencing chip 10 is kept clean, after the cell sequencing chamber 9 is cleaned, the sequencing chamber drying structure 13 is started, the heating wire 133 is electrified to generate heat, the pump body 131 works to pump the gas heated by the heating wire 133 in the communication pipeline 132 into the cell sequencing chamber 9, so that the cell sequencing chamber 9 can be dried, the self-cleaning liquid collecting structure 12 can be communicated with the inside of the device through the communication hole 123, so that the cleaned liquid can flow into the collecting tank 122, and the collecting tank connecting frame 121 can be pumped, so can take collecting box 122 that collects fully out from collecting box linking frame 121, so can conveniently handle the cancer cell waste liquid that collects with reasonable mode, avoid causing environmental pollution for whole gene sequencing device's practicality obtains fine improvement, accomplishes whole gene sequencing device's use like this.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A high throughput gene sequencing device for cancer cell gene mutation research, includes support pedestal (1), cell sequencing drive structure (6), sequencing self-cleaning structure (8), self-cleaning liquid collection structure (12) and sequencing room stoving structure (13), its characterized in that: support the upper left side of pedestal (1) and install connection hinge (3), and the top of supporting pedestal (1) is articulated between through connecting hinge (3) and apron (2), handle (4) are installed to the right-hand member face of apron (2), and the inside of apron (2) is provided with movable chamber (5), cell sequencing drive structure (6) are installed in the inside of movable chamber (5).
2. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 1, wherein: cell sequencing drive structure (6) include step motor (601), shaft joint (602), drive shaft (603) and bear dish (604), and the output of step motor (601) is connected with shaft joint (602), the output of shaft joint (602) is connected with drive shaft (603), and the top of drive shaft (603) is installed and is born dish (604) to step motor (601) pass through shaft joint (602) and drive shaft (603) and bear and constitute revolution mechanic between dish (604).
3. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 2, wherein: bear the interior angle such as dish (604) and be provided with sequencing cell and store structure (7), and sequencing cell stores structure (7) and stores jar (706) including pasting and cover diaphragm 701, heating wire (702), rotate and stir pole (703), loop bar (704), spout (705) and cell, the upper end of cell storage jar (706) is connected with pastes and covers diaphragm 701, and pastes the outside of covering diaphragm 701 and seted up spout (705) to it has heating wire (702) to inlay in the inner wall of cell storage jar (706), the inside of cell storage jar (706) is provided with loop bar (704), and the outside of loop bar (704) has cup jointed and has rotated and has stirred pole (703).
4. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 3, wherein: six groups of sequencing cell storage structures (7) are arranged, the connection mode between the pasting membrane 701) and the upper end face of the cell storage cylinder (706) is adhesive connection, and a rotating structure is formed between the rotating stirring rod (703) and the sleeve rod (704).
5. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 1, wherein: the sequencing self-cleaning structure (8) is arranged at the right lower end of the support pedestal (1), the sequencing self-cleaning structure (8) comprises a cleaning liquid tank (801), a micro hydraulic pump (802), a first sprinkler head (803) and a second sprinkler head (804), the micro hydraulic pump (802) is installed at the top end inside the cleaning liquid tank (801), the upper end of the micro hydraulic pump (802) is connected with the first sprinkler head (803) through a pipeline, and the left side of the micro hydraulic pump (802) is connected with the second sprinkler head (804) through a pipeline.
6. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 5, wherein: the cleaning liquid tank (801) is communicated with the first spray head (803) through a micro hydraulic pump (802) and a pipeline, the cleaning liquid tank (801) is communicated with the second spray head (804) through the micro hydraulic pump (802) and the pipeline, and the first spray head (803) and the cell storage cylinder (706) are perpendicular to each other.
7. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 2, wherein: bear the below of dish (604) and be provided with cell sequencing room (9), and the internally mounted of cell sequencing room (9) has slide bar (11), gene sequencing chip (10) are installed to the below of slide bar (11), and the below of gene sequencing chip (10) passes through linear connection between wire and electrical control box (16), miniature electric putter (14) are installed in the left side of cell sequencing room (9), and the top of miniature electric putter (14) is connected with baffle (15) to constitute extending structure between miniature electric putter (14) and baffle (15).
8. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 1, wherein: the self-cleaning liquid collecting structure (12) is arranged at the lower left end of the supporting pedestal (1), the self-cleaning liquid collecting structure (12) comprises a collecting box connecting frame (121), a collecting box (122) and a communicating hole (123), the collecting box (122) is arranged inside the self-cleaning liquid collecting structure (12), the communicating hole (123) penetrates through the tail end of the collecting box (122), and the collecting box connecting frame (121) is movably connected with the collecting box (122).
9. The high-throughput gene sequencing device for cancer cell gene mutation research according to claim 7, wherein: sequencing room stoving structure (13) set up in the upper left end of cell sequencing room (9), and sequencing room stoving structure (13) include the pump body (131), intercommunication pipeline (132) and heater strip (133), the right lower extreme of pump body (131) is connected with intercommunication pipeline (132), and the internally mounted of intercommunication pipeline (132) has heater strip (133) to pump body (131) link to each other between intercommunication pipeline (132) and cell sequencing room (9) and communicate with each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910528269.XA CN112094734A (en) | 2019-06-18 | 2019-06-18 | High-throughput gene sequencing device for cancer cell gene mutation research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910528269.XA CN112094734A (en) | 2019-06-18 | 2019-06-18 | High-throughput gene sequencing device for cancer cell gene mutation research |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112094734A true CN112094734A (en) | 2020-12-18 |
Family
ID=73749379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910528269.XA Pending CN112094734A (en) | 2019-06-18 | 2019-06-18 | High-throughput gene sequencing device for cancer cell gene mutation research |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112094734A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4227886A (en) * | 1976-05-13 | 1980-10-14 | National Research Development Corporation | Testing a cuvette for cleanliness |
| US4981801A (en) * | 1984-05-15 | 1991-01-01 | University Of Tokyo | Automatic cycling reaction apparatus and automatic analyzing apparatus using the same |
| US5434083A (en) * | 1989-07-17 | 1995-07-18 | Hitachi, Ltd. | Method and apparatus for automatically analyzing a plurality of test items |
| US20100234237A1 (en) * | 2005-04-30 | 2010-09-16 | Jae Chern Yoo | Bio disc, bio-driver apparatus, and assay method using the same |
| CN203123855U (en) * | 2013-03-05 | 2013-08-14 | 成都诺鑫石油科技有限公司 | Polymer swollen particle dissolution accelerating device with cleaning groove and water seepage structures |
| US20130316336A1 (en) * | 2011-02-18 | 2013-11-28 | Hitachi High-Technologies Corporation | Analyzer |
| CN203904351U (en) * | 2014-04-11 | 2014-10-29 | 珠海美路得企业发展有限公司 | Cleaning system for gene detection equipment |
| CN105316228A (en) * | 2015-10-30 | 2016-02-10 | 北京中科紫鑫科技有限责任公司 | Box-type DNA sequencer with linear feeding CCD camera |
| CN107446808A (en) * | 2017-08-23 | 2017-12-08 | 苏州思维医疗科技有限公司 | Gene sequencer |
| CN108949503A (en) * | 2018-08-03 | 2018-12-07 | 张蕊 | A kind of gene assaying device applied to biological medicine detection |
| CN111033263A (en) * | 2017-09-20 | 2020-04-17 | 深圳迈瑞生物医疗电子股份有限公司 | Automatic analysis device and working method thereof |
| CN211057117U (en) * | 2019-06-18 | 2020-07-21 | 广州市汉威信息科技有限公司 | High-throughput gene sequencing device for cancer cell gene mutation research |
-
2019
- 2019-06-18 CN CN201910528269.XA patent/CN112094734A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4227886A (en) * | 1976-05-13 | 1980-10-14 | National Research Development Corporation | Testing a cuvette for cleanliness |
| US4981801A (en) * | 1984-05-15 | 1991-01-01 | University Of Tokyo | Automatic cycling reaction apparatus and automatic analyzing apparatus using the same |
| US5434083A (en) * | 1989-07-17 | 1995-07-18 | Hitachi, Ltd. | Method and apparatus for automatically analyzing a plurality of test items |
| US20100234237A1 (en) * | 2005-04-30 | 2010-09-16 | Jae Chern Yoo | Bio disc, bio-driver apparatus, and assay method using the same |
| US20130316336A1 (en) * | 2011-02-18 | 2013-11-28 | Hitachi High-Technologies Corporation | Analyzer |
| CN203123855U (en) * | 2013-03-05 | 2013-08-14 | 成都诺鑫石油科技有限公司 | Polymer swollen particle dissolution accelerating device with cleaning groove and water seepage structures |
| CN203904351U (en) * | 2014-04-11 | 2014-10-29 | 珠海美路得企业发展有限公司 | Cleaning system for gene detection equipment |
| CN105316228A (en) * | 2015-10-30 | 2016-02-10 | 北京中科紫鑫科技有限责任公司 | Box-type DNA sequencer with linear feeding CCD camera |
| CN107446808A (en) * | 2017-08-23 | 2017-12-08 | 苏州思维医疗科技有限公司 | Gene sequencer |
| CN111033263A (en) * | 2017-09-20 | 2020-04-17 | 深圳迈瑞生物医疗电子股份有限公司 | Automatic analysis device and working method thereof |
| CN108949503A (en) * | 2018-08-03 | 2018-12-07 | 张蕊 | A kind of gene assaying device applied to biological medicine detection |
| CN211057117U (en) * | 2019-06-18 | 2020-07-21 | 广州市汉威信息科技有限公司 | High-throughput gene sequencing device for cancer cell gene mutation research |
Non-Patent Citations (2)
| Title |
|---|
| CONNELLY,: ""Paper Machine" for Molecular Diagnostics", ANALYTICAL CHEMISTRY, vol. 87, no. 15, pages 7595 - 7601, XP055330797, DOI: 10.1021/acs.analchem.5b00411 * |
| 施红: "提高DNA序列判定的准确方案", 《生物技术通讯》, vol. 12, no. 2, pages 19 - 20 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211057117U (en) | High-throughput gene sequencing device for cancer cell gene mutation research | |
| CN208098824U (en) | A kind of test tube clearing and drying device | |
| CN108325985A (en) | A kind of test tube clearing and drying device | |
| CN207479136U (en) | A kind of medical test tube cleaning storage integrated device | |
| CN112094734A (en) | High-throughput gene sequencing device for cancer cell gene mutation research | |
| CN207914281U (en) | A kind of medical test tube cleaning-drying integrated device | |
| CN111906104A (en) | Test tube cleaning device for blood collection | |
| CN210812727U (en) | Ultraviolet sterilizing box for thermometer | |
| CN107446816B (en) | Detection device suitable for PCR or HRM detection and analysis | |
| CN207444251U (en) | A kind of moveable digitlization Tobacco leaf roasting room | |
| CN110081682A (en) | Pathology are test tube cleaning drying device for experiment | |
| CN212560211U (en) | PCR gene amplification instrument for biological gene | |
| CN212856943U (en) | Cleaning device for medical instrument | |
| CN213454340U (en) | Self-cleaning solar hot water tank | |
| CN2739214Y (en) | Dyeing machine heating device | |
| CN210571619U (en) | Portable type pathology department sample preprocessing device | |
| CN210614242U (en) | Baking room for automobile | |
| CN210419915U (en) | Biological gene detection device | |
| CN209238545U (en) | A kind of pitch DSR test device for cleaning mould | |
| CN209432737U (en) | A kind of Drinking Water microbial detection device | |
| CN217568828U (en) | Analytical instrument is used in laboratory convenient to wash | |
| CN214627966U (en) | Rice seed germination box | |
| CN209669250U (en) | A kind of dry type constant incubator | |
| CN213747699U (en) | Quick drying biological tissue dyeing machine drying device | |
| CN220961014U (en) | Intelligent analysis and detection equipment for backwater quality of heat supply system of incineration power plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |