CN112090113A - Solid phase extraction equipment - Google Patents
Solid phase extraction equipment Download PDFInfo
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- CN112090113A CN112090113A CN202011058898.XA CN202011058898A CN112090113A CN 112090113 A CN112090113 A CN 112090113A CN 202011058898 A CN202011058898 A CN 202011058898A CN 112090113 A CN112090113 A CN 112090113A
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- phase extraction
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses solid phase extraction equipment, which comprises a plurality of purification columns and a box body, wherein the top end of the box body is hermetically connected with a box cover, a liquid guide pipe is connected below the box cover, the bottom ends of the purification columns penetrate through the box cover and are inserted into the top end of the liquid guide pipe, test tubes are arranged below the liquid guide pipe and are inserted into test tube holes in a fixed plate, the bottom ends of the test tubes are placed in positioning holes in a placing plate, a first threaded hole is formed in the fixed plate, a second threaded hole is formed in the placing plate, screw rods respectively penetrate through the first threaded hole and the second threaded hole and are supported on a bottom plate of the box body, the side wall of. The waste liquid groove is arranged in the box body, so that the waste liquid can be prevented from spilling to pollute the experimental apparatus and the experimental table; through setting up the purifying column adapter, solved the problem of two purifying columns series connection, satisfied the requirement that some detection methods often can establish ties two purifying columns, obtained fine effect.
Description
Technical Field
The invention relates to an extraction device, in particular to solid phase extraction equipment, and belongs to the technical field of detection analysis assay equipment.
Background
Solid Phase Extraction (SPE) is a sample pretreatment technology developed in the late 80 s of the 20 th century, and is based on the principle of liquid chromatography to separate sample components, i.e. the sample is enriched and purified by a solid adsorbent. Since the solid phase extraction technology is developed and commercialized, the method is widely applied to the fields of medicine, food, environment, commodity inspection, chemical industry and the like.
Present widely used solid phase extraction equipment has certain limitation, place the sample extract generally and extract the operation in the purifying column, utilize the piston to apply the mode of malleation, let the sample extract reach the extraction effect through purifying column, when adding the sample washing liquid in the purifying column every time, just need take off the piston, the operation degree of difficulty has been increased, and the easy outflow of scrubbing solvent pollutes the laboratory bench, the cross contact of piston and sample extract, can cause all kinds of pollutants to remain, thereby the pollution detection object.
Moreover, the waste liquid tank of the existing solid phase extraction equipment is inconvenient to replace, so that the waste liquid is often spilled to pollute the laboratory appliances and the laboratory bench and influence the sample detection result.
With the rapid development of the detection industry, various detection standards are continuously increased, many new detection standards are continuously generated, and because common solid phase extraction equipment cannot meet the experiment requirement that some detection methods need to be connected with two purification columns in series, two times of purification is often required, which is time-consuming and labor-consuming.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the solid phase extraction equipment is provided, and the waste liquid tank is arranged in the box body, so that the waste liquid can be prevented from spilling to pollute experimental appliances and an experimental bench; through setting up the purification column adapter, solved the problem of two purification column series connections, satisfied some detection methods and can establish ties two purification columns often, and the experiment requirement that ordinary solid phase extraction equipment can't satisfy often needs to carry out twice and purifies, not only wastes time but also the technical problem who wastes energy.
The technical scheme of the invention is as follows: the utility model provides a solid phase extraction equipment, it includes a plurality of purification posts, it still includes the box, box top sealing connection case lid, the catheter is connected to the case lid below, it inserts the catheter top to pass the case lid to purify the post bottom, establish the test tube below the catheter, the test tube inserts in the test tube hole on the fixed plate, the test tube bottom is placed and is placed in the locating hole on placing the board, establish screw hole one on the fixed plate, place and establish screw hole two on the board, the screw rod passes screw hole one respectively and supports to the box bottom plate with screw hole two on, through vacuum tube connection vacuum pump on the box lateral wall, be equipped with vacuum valve and vacuum meter on.
The support columns are fixedly connected to the side walls of the box body, and the bases are connected to the lower portions of four corners of the bottom plate of the box body.
And a discharge pipe is arranged on the side wall of the bottom end of the box body, and a discharge valve is arranged on the discharge pipe.
The cylinder of purification post inserts the fixed orifices on the purification post fixed plate, purification post fixed plate horizontal arrangement and both sides fixedly connected with position sleeve, and the position sleeve passes through locking screw to be fixed to the support column.
And a second purifying column is connected in series above the purifying column through an adapter.
The adapter is drawing pin shape structure, and the centre of adapter is equipped with the through-hole, and the second purifies in the bottom of post inserts the through-hole, and the adapter bottom is the cylinder, and the cylinder inserts and carries out the location connection in the cavity at the top of purifying the post.
The side below all around of case lid is equipped with the recess, and the lateral wall top of box is located the recess and passes through sealed pad and case lid sealing connection.
The invention has the beneficial effects that: compared with the prior art, the technical scheme of the invention has the advantages that different purification columns are selected for installation according to purification modes in different detection methods, and the purification modes are easily and conveniently changed. The device has the effects of convenient operation, simple structure and strong applicability. The gas in the box body is pumped out through the vacuum pump, so that negative pressure is formed in the box body, the sample extracting solution achieves a purifying effect through assembling the purifying column under the action of atmospheric pressure and the negative pressure of the box body, the problem that a detection object is polluted because the piston needs to be taken down when sample washing liquid is added into the purifying column every time in the process of extracting a sample by utilizing the mode that the piston applies positive pressure is solved, the operation difficulty is increased, a washing solvent easily flows out to pollute an experiment table, and various pollutants are left due to the cross contact of the piston and the sample extracting solution; the waste liquid tank is arranged in the box body (the bottom of the box body is the waste liquid tank), so that the waste liquid can be prevented from spilling to pollute experimental appliances and an experimental table; through setting up the purification column adapter, solved the problem of two purification column series connections, satisfied some detection methods and can establish ties two purification columns often, and the experiment requirement that ordinary solid phase extraction equipment can't satisfy often needs to carry out twice and purifies, not only wastes time but also the technical problem that difficultly, has gained fine result of use.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a top view of the present invention;
FIG. 3 is an enlarged partial cross-sectional view of the invention A;
figure 4 is a cross-sectional view of an adapter of the present invention;
figure 5 is a top view of an adapter of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.
Example 1: as shown in fig. 1 to 5, a solid phase extraction device comprises a plurality of purification columns 2, and further comprises a box body 17, wherein the top end of the box body 17 is hermetically connected with a box cover 7, a liquid guide pipe 9 is connected below the box cover 7, the bottom ends of the purification columns 2 penetrate through the box cover 7 and are inserted into the top end of the liquid guide pipe 9, test tubes 11 are arranged below the liquid guide pipe 9, the test tubes 11 are inserted into test tube holes 10 in a fixing plate 6, the bottom ends of the test tubes 11 are placed in positioning holes 13 in a placing plate 14, a first threaded hole 16 is formed in the fixing plate 6, a second threaded hole 12 is formed in the placing plate 14, screws 18 respectively penetrate through the first threaded hole 16 and the second threaded hole 12 and are supported on a bottom plate of the box body 17, a vacuum pump.
The diameter of the positioning hole 13 is smaller than the diameter of the test tube hole 10. The positioning hole 13 can make the waste liquid flow into the bottom of the box body 17 for centralized storage and then discharge the waste liquid to the outside of the box body 17 through the discharge pipe 25.
The height of the distance between the fixing plate 6 and the placing plate 14 can be adjusted by the screw 18 to meet the model of the test tube 11 with different specifications.
The vacuum degree inside the box 17 can be adjusted by the vacuum valve 24, and the degree of vacuum can be read by observing the vacuum gauge 22, thereby controlling the flow rate of the sample extracting solution through the purifying column 2.
The middle of the liquid guide tube 9 is provided with a small hole 8, and the bottom end of the purifying column 2 is inserted into the small hole 8 for positioning.
Furthermore, a supporting column 19 is fixedly connected to the side wall of the box body 17, and bases 15 are connected to the lower portions of four corners of a bottom plate of the box body 17.
Further, a discharge pipe 25 is provided on the side wall of the bottom end of the tank 17, and a discharge valve 26 is provided on the discharge pipe 25. The waste liquid after the experiment can be discharged to the outside of the tank 17.
Further, the column body of the purifying column 2 is inserted into the fixing hole 27 on the purifying column fixing plate 1, the purifying column fixing plate 1 is horizontally arranged, the two sides of the purifying column fixing plate 1 are fixedly connected with the positioning sleeves 4, and the positioning sleeves 4 are fixed on the supporting column 19 through the locking screws 5.
Further, a second purifying column 28 is connected in series above the purifying column 2 through a joint 3. Whether the second purification column 28 needs to be connected in series is determined by the needs of the experiment.
Further, adapter 3 is drawing pin shape structure, and the centre of adapter 3 is equipped with through-hole 31, and the bottom of second purification post 28 is inserted in through-hole 31, and adapter 3 bottom is cylinder 32, and cylinder 32 inserts and carries out the location connection in the cavity at the top of purifying post 2. The adapter 3 greatly improves the range and the experiment precision of the experiment by connecting the purifying column 2 and the second purifying column 28 together in series and in a sealing way.
Furthermore, a groove 21 is formed in the lower side of the periphery of the box cover 7, and the top end of the side wall of the box body 17 is located in the groove 21 and is connected with the box cover 7 in a sealing mode through a sealing gasket 20.
Place board 14 and test tube fixed plate 6 through being equipped with detachable in box 17 inside, can dismantle and place board 14 parallel bit in 6 downside of test tube fixed plate, it has test tube hole 10 to open on test tube fixed plate 6, and the test tube passes test tube hole 10 and is fixed in on test tube fixed plate 6, be equipped with test tube hole 10 one-to-one on catheter 9 and the test tube fixed plate on case lid 7, concrete simple structure, convenient operation, effect that the practicality is strong!
The working principle is as follows: when the device is used, the placing plate 14 and the test tube fixing plate 6 penetrate through the first threaded hole 16 and the second threaded hole 12 through the studs 18 to be supported on the bottom plate of the box body 17, the placing plate 14 is positioned at the lower side of the test tube fixing plate 6 in parallel, the test tube 11 is placed on the placing plate 14 in the box body 17 and is fixed in the vertical direction through the test tube fixing plate 6, the box body 17 is covered by the box cover 7, the liquid guide tubes 9 correspond to the test tube 11 one by one, the purifying column fixing plate 1 is fixed on the support column 19 through the locking screws 5 according to the height of the purifying column 2, the purifying column 2 and the second purifying column 28 are connected through the adapter 3 to form a series-connected column, the series-connected column is inserted on the liquid guide tube 9 and is fixed through the purifying column fixing plate 1, the vacuum pump is switched on for the device, so that the device obtains negative pressure, the device can normally operate, when the solid phase extraction equipment operates, the sample extracting solution flows through the series purification columns under the action of the atmospheric pressure and the negative pressure difference in the box body 17 to achieve the purification effect, and the vacuum valve 24 is adjusted to adjust the internal and external pressure difference in the box body 17, so that the flow rate of the sample extracting solution can be adjusted to carry out purification operation.
The problem of the extraction effect is achieved by using a piston to apply positive pressure in the sample extraction process, the piston needs to be taken down when a sample washing liquid is added into the purification column every time, the operation difficulty is increased, the washing solvent easily flows out to pollute an experiment table, and various pollutants are left due to the cross contact between the piston and the sample extraction liquid, so that a detection object is polluted; moreover, the waste liquid tank of the existing solid phase extraction equipment is inconvenient to replace, so that the waste liquid is often spilled to pollute the laboratory apparatus and the laboratory bench and influence the detection result of the sample; because some detection methods can often connect two purifying columns in series, common solid phase extraction equipment can not meet the experimental requirements, and two times of purification is often required, which is a technical problem of time and labor waste.
The present invention is not described in detail, but is known to those skilled in the art. Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.
Claims (7)
1. A solid phase extraction equipment, it includes a plurality of purifying columns (2), its characterized in that: it still includes box (17), box (17) top sealing connection case lid (7), liquid pipe (9) are connected to case lid (7) below, it inserts liquid pipe (9) top to pass case lid (7) to purify post (2) bottom, establish test tube (11) in liquid pipe (9) below, test tube (11) are inserted in test tube hole (10) on fixed plate (6), place in locating hole (13) on placing board (14) test tube (11) bottom, establish screw hole one (16) on fixed plate (6), place and establish screw hole two (12) on board (14), screw rod (18) pass screw hole one (16) and screw hole two (12) respectively and support on box (17) bottom plate, connect the vacuum pump through vacuum tube (23) on box (17) lateral wall, be equipped with vacuum valve (24) and vacuum meter (22) on vacuum tube (23).
2. The solid phase extraction apparatus of claim 1, wherein: fixedly connected with support column (19) on box (17) lateral wall, box (17) bottom plate four corners below is connected with base (15).
3. The solid phase extraction apparatus of claim 1, wherein: and a discharge pipe (25) is arranged on the side wall of the bottom end of the box body (17), and a discharge valve (26) is arranged on the discharge pipe (25).
4. The solid phase extraction apparatus of claim 1, wherein: the cylinder of purifying column (2) inserts fixed orifices (27) on purifying column fixed plate (1) in, purifying column fixed plate (1) horizontal arrangement and both sides fixedly connected with position sleeve (4), and position sleeve (4) are fixed to support column (19) through locking screw (5).
5. The solid phase extraction apparatus of claim 1 or 4, wherein: and a second purifying column (28) is connected in series above the purifying column (2) through an adapter (3).
6. The solid phase extraction apparatus of claim 5, wherein: adapter (3) are drawing pin shape structure, and the centre of adapter (3) is equipped with through-hole (31), and in through-hole (31) were inserted to the bottom of second purification post (28), adapter (3) bottom was cylinder (32), and cylinder (32) are inserted and are fixed a position in the top cavity of purification post (2) and connect.
7. The solid phase extraction apparatus of claim 1, wherein: the box cover (7) is provided with grooves (21) on the lower side all around, and the top end of the side wall of the box body (17) is positioned in the grooves (21) and is hermetically connected with the box cover (7) through a sealing gasket (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011058898.XA CN112090113A (en) | 2020-09-30 | 2020-09-30 | Solid phase extraction equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011058898.XA CN112090113A (en) | 2020-09-30 | 2020-09-30 | Solid phase extraction equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112090113A true CN112090113A (en) | 2020-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011058898.XA Pending CN112090113A (en) | 2020-09-30 | 2020-09-30 | Solid phase extraction equipment |
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| CN (1) | CN112090113A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092430A (en) * | 2021-04-09 | 2021-07-09 | 青岛复诺生物医疗有限公司 | Sperm active oxygen content measuring device and measuring method thereof |
| CN113750570A (en) * | 2021-08-02 | 2021-12-07 | 佛山科学技术学院 | Rotatable solid phase extraction device |
| CN114034537A (en) * | 2021-09-16 | 2022-02-11 | 拉萨海关技术中心 | Solid phase extraction equipment for food detection and separation method |
| CN116448535A (en) * | 2023-06-16 | 2023-07-18 | 杭州凯莱谱质造科技有限公司 | Full-automatic magnetic solid phase extraction equipment |
-
2020
- 2020-09-30 CN CN202011058898.XA patent/CN112090113A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092430A (en) * | 2021-04-09 | 2021-07-09 | 青岛复诺生物医疗有限公司 | Sperm active oxygen content measuring device and measuring method thereof |
| CN113092430B (en) * | 2021-04-09 | 2022-06-28 | 青岛复诺生物医疗有限公司 | Sperm active oxygen content measuring device and measuring method thereof |
| CN113750570A (en) * | 2021-08-02 | 2021-12-07 | 佛山科学技术学院 | Rotatable solid phase extraction device |
| CN114034537A (en) * | 2021-09-16 | 2022-02-11 | 拉萨海关技术中心 | Solid phase extraction equipment for food detection and separation method |
| CN116448535A (en) * | 2023-06-16 | 2023-07-18 | 杭州凯莱谱质造科技有限公司 | Full-automatic magnetic solid phase extraction equipment |
| CN116448535B (en) * | 2023-06-16 | 2023-11-10 | 杭州凯莱谱质造科技有限公司 | Full-automatic magnetic solid phase extraction equipment |
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