CN112067554A - Dynamic observation device and method for centrifugal machine - Google Patents
Dynamic observation device and method for centrifugal machine Download PDFInfo
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- CN112067554A CN112067554A CN202011058112.4A CN202011058112A CN112067554A CN 112067554 A CN112067554 A CN 112067554A CN 202011058112 A CN202011058112 A CN 202011058112A CN 112067554 A CN112067554 A CN 112067554A
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- centrifuge
- transparent
- light source
- image collector
- position sensor
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000005119 centrifugation Methods 0.000 claims description 14
- 239000004743 Polypropylene Substances 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 abstract description 18
- 239000008280 blood Substances 0.000 abstract description 18
- 239000012503 blood component Substances 0.000 abstract description 4
- 239000013589 supplement Substances 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 210000004700 fetal blood Anatomy 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- H—ELECTRICITY
- H05—ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
- H05B—ELECTRIC HEATING; ELECTRIC LIGHT SOURCES NOT OTHERWISE PROVIDED FOR; CIRCUIT ARRANGEMENTS FOR ELECTRIC LIGHT SOURCES, IN GENERAL
- H05B45/00—Circuit arrangements for operating light-emitting diodes [LED]
- H05B45/30—Driver circuits
- H05B45/305—Frequency-control circuits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention relates to the technical field of blood component treatment, and discloses a dynamic observation device and a dynamic observation method for a centrifugal machine, wherein the dynamic observation device comprises a transparent centrifugal tube; an image collector; a light source; a bucket; a rotor position sensor; and a strobe trigger module. The invention also discloses a dynamic observation method for the centrifuge, which comprises the following steps: s1, operating the centrifuge; s2, the rotor position sensor generates an angle position signal and transmits the angle position signal to the stroboscopic trigger module, and the stroboscopic trigger module lights the light source once; s3, collecting the instantaneous picture of the transparent centrifugal tube by an image collector; s4, the image collector transmits the instantaneous picture to the display; and S5, visually observing the instantaneous picture to judge the centrifugal state of the sample. According to the invention, the stroboscopic trigger module triggers the light source to supplement light for the image collector, so that the image collector can capture clear instantaneous images of the transparent centrifugal tube, and an operator can conveniently judge the centrifugal progress of the blood sample.
Description
Technical Field
The invention relates to the technical field of blood component processing, in particular to a dynamic observation device and method for a centrifugal machine.
Background
The current cell technology is developed rapidly, and the research and application of the cell technology still have a large space. The essential step in cell technology-related research and applications is the acquisition of stem cells or immune cells. Such as cord blood stem cells and peripheral blood mononuclear cells, by centrifuging cord blood or peripheral blood using a centrifugation technique. The quality and efficiency of centrifugation directly affect the results of related research work and applications.
The quality of blood centrifugation is affected in many ways by the centrifuge factor. Generally, the more stable the centrifuge runs, the more appropriate the centrifugal force, and the more reasonable the centrifugal time is, the more the quality of blood centrifugation can be controlled. In reality, the separation factor used in the same blood separation procedure is always constant, and the individual blood sample sources are different, which results in that the empirical separation factor is not well adapted to the different individual blood sample sources, and thus the quality of partial blood separation is not good and even the waste of blood samples is caused.
At present, a centrifuge is at a high rotating speed in the operation process of centrifugation, and a centrifuge tube moving at a high speed and a blood sample in the centrifuge tube cannot be directly observed. Generally, only one method for knowing whether the centrifugal work is finished is to stop the centrifuge, however, the start and stop of the centrifuge are generally slow and smooth to facilitate the separation of components, which is time-consuming and tedious. The operator is not able to observe the change in the blood sample in the centrifuge tube during the time that the centrifuge is started and stopped.
Disclosure of Invention
The present invention is directed to a dynamic observation device and method for a centrifuge, which solves the above problems of the related art.
In order to achieve the above object, in one aspect, the present invention provides the following technical solutions: a dynamic observation device and method for a centrifuge comprise
The transparent centrifugal tube is used for storing samples;
the image collector is positioned above the transparent centrifugal tube and used for obtaining an image of the layered state of the sample in the transparent centrifugal tube;
the light source is positioned below the transparent centrifugal tube and used for supplementing light for the image collector;
the hanging bucket is fixedly arranged on a rotor of the centrifugal machine and used for storing the transparent centrifugal tube and allowing light rays generated by the light source to penetrate through;
the rotor position sensor is fixedly arranged on the rotor and used for acquiring an angle position signal of the rotor;
and the stroboscopic trigger module is fixedly arranged in the centrifugal machine, is used for receiving the signal from the rotor position sensor and is electrically connected with the input end of the light source.
Optionally, the transparent centrifuge tube comprises a polypropylene centrifuge tube; the bucket comprises a transparent centrifuge tube bucket or a windowed centrifuge tube bucket.
Optionally, the rotor position sensor includes any one of a hall element type position sensor, an electromagnetic type position sensor, and a photoelectric type position sensor.
Optionally, the strobe trigger module includes a working power supply and a strobe controller; the output voltage of the working power supply is 5V, 12V, 24V and 28V; the width of the output voltage pulse of the stroboscopic controller is 0-1000 us.
Optionally, the light source includes a white LED array light source, and the luminance of output light of the white LED array light source is 15000-20000 mcd.
Optionally, the lens of the image collector comprises a wide-angle lens, and the wide-angle lens covers the length of the transparent centrifugal tube; the output end of the image collector 7 is electrically connected with the display.
On the other hand, the invention also provides the following technical scheme: a dynamic viewing method for a centrifuge, comprising the steps of:
s1, operating the centrifuge with certain centrifugal force parameters;
s2, the rotor position sensor generates an angle position signal and transmits the angle position signal to the stroboscopic trigger module, and the stroboscopic trigger module lights the light source once;
s3, collecting the instantaneous picture of the transparent centrifugal tube by an image collector;
s4, the image collector transmits the instantaneous picture to the display, and the display displays the instantaneous picture;
and S5, visually observing the instantaneous picture by an operator, and judging the centrifugation state of the sample.
Compared with the prior art, the invention provides a dynamic observation device and a method for a centrifugal machine, which have the following beneficial effects: according to the invention, the stroboscopic trigger module triggers the light source to supplement light for the image collector, so that the image collector can capture clear instantaneous images of the transparent centrifuge tube, operators can conveniently judge the centrifugation progress of blood samples, the influence of individual differences in the blood samples of different individuals is eliminated, and the optimal blood centrifugation quality is obtained.
Drawings
FIG. 1 is a schematic view of the apparatus of the present invention.
In the figure: the device comprises a centrifuge 1, a bucket 2, a transparent centrifuge tube 3, a rotor position sensor 4, a stroboscopic trigger module 5, a light source 6, an image collector 7 and a display 8.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b): referring to fig. 1, the present invention provides a dynamic observation device for a centrifuge, which includes a transparent centrifuge tube 3 for storing a blood sample; the image collector 7 is positioned above the transparent centrifugal tube 3 and is used for obtaining an image of the layered state of the sample in the transparent centrifugal tube 3; the light source 6 is positioned below the transparent centrifugal tube 3 and used for supplementing light for the image collector 7; the hanging bucket 2 is fixedly arranged on the rotor of the centrifuge 1 and used for storing the transparent centrifuge tube 3 and allowing light rays generated by the light source 6 to penetrate through; the rotor position sensor 4 is fixedly arranged on the rotor and used for acquiring an angle position signal of the rotor; and the stroboscopic trigger module 5 is fixedly arranged in the centrifuge 1, is used for receiving a signal from the rotor position sensor 4, and is electrically connected with the input end of the light source 6.
Wherein, the centrifugal machine 1 is a swing-out centrifugal machine. The transparent centrifugal tube 3 is a polypropylene centrifugal tube and has high transparency; the bucket 2 is a transparent centrifuge tube bucket. The rotor position sensor 4 is a hall element type position sensor. The stroboscopic trigger module 5 comprises a working power supply and a stroboscopic controller; the output voltage of working power supply is 5V, 12V, 24V, 28V, and working power supply is rotor position sensor 4, stroboscopic trigger module 5 and the energy supply of light source 6, and the width of stroboscopic controller output voltage pulse is 0 ~ 1000 us. The light source 6 is a white light LED array light source, and the brightness of output light of the white light LED array light source is 15000-20000 mcd. The lens of the image collector 7 is a wide-angle lens, and the wide-angle lens covers the length of the transparent centrifuge tube 3; the output end of the image collector 7 is electrically connected with the display 8.
Stroboscopic trigger module 5 chooses FST-SCP24V96T4 stroboscopic controller, and for the technology known in the art, stroboscopic trigger module 5 lights light source 6 once every time stroboscopic trigger module 5 receives a signal from rotor position sensor 4, so that image collector 7 captures a clear instantaneous picture of transparent centrifuge tube 3.
The invention provides a dynamic observation method of a centrifuge, which comprises the following steps:
s1, operating the centrifuge 1 with certain centrifugal force parameters;
s2, the rotor position sensor 4 generates an angle position signal and transmits the angle position signal to the strobe trigger module 5, and the strobe trigger module 5 lights the light source 6 once;
s3, collecting the instantaneous picture of the transparent centrifuge tube 3 by the image collector 7;
s4, the image collector 7 transmits the instantaneous picture to the display 8, and the display 8 displays the instantaneous picture;
and S5, visually observing the instantaneous picture by an operator, and judging the centrifugation state of the sample.
Dynamic observation principle: after the centrifuge 1 operates, the rotor position sensor 4 generates a rotor position signal for triggering the stroboscopic triggering module 5, and the stroboscopic triggering module 5 receives the rotor angle position signal and then lights the light source 6 once. At this time, the bucket 2 and the transparent centrifuge tube 3 containing the blood sample in the centrifugal state are just under the image collector 7, and the image collector 7 collects bright and clear instantaneous pictures under the action of light supplement of the light source 6.
When the centrifuge 1 is still running with fixed centrifugal force parameters, the light source 6 is lighted once when the rotor of the centrifuge 1 reaches the same position every time, and the instantaneous centrifugal picture of the bright and clear transparent centrifuge tube 3 is collected by the image collector 7. The series of instantaneous centrifugation pictures are displayed through the display 8, and an operator can observe and judge the centrifugation state of the blood sample in the transparent centrifuge tube 3 in the centrifugation process at any time through naked eyes until the instantaneous pictures show that different blood components are layered obviously, and then the operator can judge that the blood sample centrifugation is finished.
Because the time interval of the instantaneous pictures of the transparent centrifuge tube 3 collected by the image collector 7 is short (the time interval is determined by the rotating speed of the centrifuge), the change of the collected images is also small, the instantaneous pictures collected by the image collector 7 are continuously played to form a group of video streams, and the video streams can indicate that blood samples in the transparent centrifuge tube 3 are gradually layered from an initial state in the operation process of the centrifuge 4, and finally, a complete process of different blood component layered clear states is formed.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A dynamic observation device for a centrifuge, characterized in that: comprises that
A transparent centrifuge tube (3) for storing samples;
the image collector (7) is positioned above the transparent centrifugal tube (3) and is used for obtaining an image of the layered state of the sample in the transparent centrifugal tube (3);
the light source (6) is positioned below the transparent centrifugal tube (3) and used for supplementing light for the image collector (7);
the hanging barrel (2) is fixedly arranged on a rotor of the centrifuge (1) and is used for storing the transparent centrifuge tube (3) and allowing light rays generated by the light source (6) to penetrate through;
the rotor position sensor (4) is fixedly arranged on the rotor and used for acquiring an angle position signal of the rotor;
and the stroboscopic trigger module (5) is fixedly arranged in the centrifuge (1) and is used for receiving the signal from the rotor position sensor (4) and is electrically connected with the input end of the light source (6).
2. A dynamic viewing device for a centrifuge according to claim 1, wherein: the transparent centrifuge tube (3) comprises a polypropylene centrifuge tube; the bucket (2) comprises a transparent centrifuge tube bucket or a windowed centrifuge tube bucket.
3. A dynamic viewing device for a centrifuge according to claim 1, wherein: the rotor position sensor (4) comprises any one of a Hall element type position sensor, an electromagnetic type position sensor and a photoelectric type position sensor.
4. A dynamic viewing device for a centrifuge according to claim 1, wherein: the stroboscopic trigger module (5) comprises a working power supply and a stroboscopic controller; the output voltage of the working power supply is 5V, 12V, 24V and 28V; the width of the output voltage pulse of the stroboscopic controller is 0-1000 us.
5. A dynamic viewing device for a centrifuge according to claim 1, wherein: the light source (6) comprises a white light LED array light source, and the brightness of output light of the white light LED array light source is 15000-20000 mcd.
6. A dynamic viewing device for a centrifuge according to claim 1, wherein: the lens of the image collector (7) comprises a wide-angle lens, and the wide-angle lens covers the length of the transparent centrifugal tube (3); the output end of the image collector (7) is electrically connected with the display (8).
7. A dynamic observation method for a centrifuge, characterized in that: the method comprises the following steps:
s1, operating the centrifuge (1) with certain centrifugal force parameters;
s2, the rotor position sensor (4) generates an angle position signal and transmits the angle position signal to the stroboscopic trigger module (5), and the stroboscopic trigger module (5) lights the light source (6) once;
s3, collecting the instantaneous picture of the transparent centrifugal tube (3) by an image collector (7);
s4, the image collector (7) transmits the instantaneous picture to the display (8), and the display (8) displays the instantaneous picture;
and S5, visually observing the instantaneous picture by an operator, and judging the centrifugation state of the sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011058112.4A CN112067554A (en) | 2020-09-30 | 2020-09-30 | Dynamic observation device and method for centrifugal machine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011058112.4A CN112067554A (en) | 2020-09-30 | 2020-09-30 | Dynamic observation device and method for centrifugal machine |
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| CN112067554A true CN112067554A (en) | 2020-12-11 |
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| CN202011058112.4A Pending CN112067554A (en) | 2020-09-30 | 2020-09-30 | Dynamic observation device and method for centrifugal machine |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080045394A1 (en) * | 2006-08-17 | 2008-02-21 | Gambro Bct, Inc. | Blood Processing Apparatus with Robust Automated Process Control |
| CN102348985A (en) * | 2009-03-09 | 2012-02-08 | 三星电子株式会社 | Strobo thin film chemical analysis apparatus and assay method using the same |
| CN102879399A (en) * | 2012-09-25 | 2013-01-16 | 西安交通大学 | Red blood cell and solid wall surface high-speed collision microscopic visual experiment device |
| CN103635209A (en) * | 2011-09-26 | 2014-03-12 | 汾沃有限公司 | Optical monitoring system for blood processing system |
| CN106659825A (en) * | 2014-08-19 | 2017-05-10 | 瑞帕利克斯有限公司 | Centrifuge and method of centrifuging a blood sample |
| CN108885201A (en) * | 2015-09-29 | 2018-11-23 | 美国血液技术公司 | System and method for rotating object to be imaged |
| CN110643565A (en) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | High-reliability automatic cell separation method and device based on visual identification |
| CN212301290U (en) * | 2020-09-30 | 2021-01-05 | 金西盟(天津)人工智能有限责任公司 | Dynamic observation device for centrifugal machine |
-
2020
- 2020-09-30 CN CN202011058112.4A patent/CN112067554A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080045394A1 (en) * | 2006-08-17 | 2008-02-21 | Gambro Bct, Inc. | Blood Processing Apparatus with Robust Automated Process Control |
| CN101501472A (en) * | 2006-08-17 | 2009-08-05 | 科安比司特公司 | Blood treatment apparatus with robust automated process control |
| CN102348985A (en) * | 2009-03-09 | 2012-02-08 | 三星电子株式会社 | Strobo thin film chemical analysis apparatus and assay method using the same |
| CN103635209A (en) * | 2011-09-26 | 2014-03-12 | 汾沃有限公司 | Optical monitoring system for blood processing system |
| CN102879399A (en) * | 2012-09-25 | 2013-01-16 | 西安交通大学 | Red blood cell and solid wall surface high-speed collision microscopic visual experiment device |
| CN106659825A (en) * | 2014-08-19 | 2017-05-10 | 瑞帕利克斯有限公司 | Centrifuge and method of centrifuging a blood sample |
| CN108885201A (en) * | 2015-09-29 | 2018-11-23 | 美国血液技术公司 | System and method for rotating object to be imaged |
| CN110643565A (en) * | 2018-06-26 | 2020-01-03 | 深圳市北科生物科技有限公司 | High-reliability automatic cell separation method and device based on visual identification |
| CN212301290U (en) * | 2020-09-30 | 2021-01-05 | 金西盟(天津)人工智能有限责任公司 | Dynamic observation device for centrifugal machine |
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