CN112048401B - Micro-fluidic chip cleaning agent and method thereof - Google Patents
Micro-fluidic chip cleaning agent and method thereof Download PDFInfo
- Publication number
- CN112048401B CN112048401B CN202010887325.1A CN202010887325A CN112048401B CN 112048401 B CN112048401 B CN 112048401B CN 202010887325 A CN202010887325 A CN 202010887325A CN 112048401 B CN112048401 B CN 112048401B
- Authority
- CN
- China
- Prior art keywords
- micro
- chip
- cleaning agent
- fluidic chip
- fluidic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000012459 cleaning agent Substances 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000004140 cleaning Methods 0.000 claims abstract description 28
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000010355 oscillation Effects 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229960001484 edetic acid Drugs 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- FYFFGSSZFBZTAH-UHFFFAOYSA-N methylaminomethanetriol Chemical compound CNC(O)(O)O FYFFGSSZFBZTAH-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229940049964 oleate Drugs 0.000 claims abstract description 4
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims abstract description 4
- 239000003292 glue Substances 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 21
- 239000011259 mixed solution Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000012901 Milli-Q water Substances 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 description 17
- 239000007788 liquid Substances 0.000 description 15
- 239000000203 mixture Substances 0.000 description 11
- 239000000523 sample Substances 0.000 description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 210000003811 finger Anatomy 0.000 description 4
- 210000005224 forefinger Anatomy 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 210000003813 thumb Anatomy 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 238000009472 formulation Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 229910021642 ultra pure water Inorganic materials 0.000 description 3
- 239000012498 ultrapure water Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000003260 vortexing Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- -1 oleic acid ester Chemical class 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000012430 stability testing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/32—Organic compounds containing nitrogen
- C11D7/3245—Aminoacids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/265—Carboxylic acids or salts thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/26—Organic compounds containing oxygen
- C11D7/266—Esters or carbonates
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/32—Organic compounds containing nitrogen
- C11D7/3218—Alkanolamines or alkanolimines
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Emergency Medicine (AREA)
- Detergent Compositions (AREA)
Abstract
The invention relates to the technical field of biology, in particular to a micro-fluidic chip cleaning agent and a method thereof, wherein the cleaning agent comprises the following components in parts by mass: 10-20 parts of ethylene diamine tetraacetic acid, 300 parts of trihydroxymethyl aminomethane 200-acetic acid, 30-80 parts of acetic acid and 0.1-10 parts of oleate; the method comprises adding 10-20ul MilliQ water into the holes of the microfluidic chip, mixing at low speed, and oscillating; holding two sides of the chip, and knocking the microfluidic chip downwards until all the solution in the microfluidic chip hole flows out; adding the micro-fluidic chip cleaning agent into micro-fluidic chip holes respectively, performing vortex oscillation, and removing the solution in the micro-fluidic chip holes to obtain a clean micro-fluidic chip; the invention provides a micro-fluidic chip cleaning agent which is clean in cleaning, free of residues and convenient to operate and a method thereof.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a micro-fluidic chip cleaning agent and a method thereof.
Background
The microfluidic chip has the characteristics of controllable fluid flow, extremely less consumed samples and reagents, high analysis speed and the like, so compared with the traditional agarose and polyacrylamide gel electrophoresis, the electrophoresis based on the microfluidic chip has the advantages of less required sample loading amount, simple and time-saving operation and better performance. At present, microfluidic chips are widely applied to biological experiments such as nucleic acid protein electrophoresis. However, microfluidic chip processing is very expensive and the technology is almost monopolized in europe and america.
There have been successive articles reporting reagent formulations and cleaning methods developed in laboratories for cleaning chips. In the reported patents and literature, the formulation of cleaning agents mostly adopts low pH value ()<4) H of (A) to (B)2O2Or a sodium ion buffer such as sodium hypochlorite with the addition of a suitable DNAse, or a high pH value: (>10) The NaOH solution is also used for sequentially cleaning the regeneration chip by the two formula reagents at intervals. In addition, since the diameter of the lanes of the microfluidic chip is in the order of micrometers, most of the cleaning methods employ pumps or negative pressure.
As shown in FIG. 1, the chip is cleaned by using a conventional cleaning reagent 0.5M NaOH and a negative pressure pump in the prior art. From the results, it is understood that the chip washed with 0.5M NaOH causes degradation of RNA Ladder.
In summary, the method for cleaning the chip in the prior art has two obvious defects, 1, the residual cleaning solution can degrade the nucleic acid/protein of the subsequent experiment sample; 2. the chip is cleaned by sucking in the cleaning liquid by means of a pump or negative pressure, which is inconvenient in practical operation and is not available in every laboratory.
MilliQ water, which is ultrapure water, is produced by Milli-Q Academic A10 ultrapure water system from Bai Millipore corporation, USA. An ultrapure water system of the Milli-Q Biocel type, zhi Millipore, USA. Most suitable for cell culture.
Disclosure of Invention
Aiming at the problems, the invention discloses a micro-fluidic chip cleaning agent which is clean in cleaning, free of residue and convenient to operate and a method thereof.
The invention provides a micro-fluidic chip cleaning agent, which comprises the following components in parts by mass:
preferably, the pH of the ethylenediaminetetraacetic acid is 7.5-8.5.
Preferably, the PH of the micro-fluidic chip cleaning agent is 7-8.
Preferably, the cleaning agent is an aqueous agent.
According to the invention, the traditional low-pH-value acid or high-pH-value alkaline buffer solution is changed, and the cleaning agent with pH of 7-8 is selected, so that the degradation interference of the cleaning agent on the subsequent nucleic acid protein sample is completely avoided, and the real data result is influenced; the oleic acid ester non-ionic detergent is added into the formula of the cleaning reagent, so that the protein can be emulsified in a biological experiment, the structure of the protein is not damaged, and the damage to the original interaction between the proteins can be reduced.
In a second aspect of the invention, a method for preparing a cleaning agent for a microfluidic chip is provided, which comprises the following steps:
1.1) preparing ethylenediamine tetraacetic acid to ensure that the PH value is 7.5-8.5;
1.2) adding trihydroxymethyl aminomethane into acetic acid to form a mixed solution;
1.3) adding the ethylene diamine tetraacetic acid prepared in the step 1.1) into the mixed solution obtained in the step 1.2);
1.4) adding oleate into the mixed solution obtained in the step 1.3), and adjusting the pH value to 7-8 to obtain the micro-fluidic chip cleaning agent.
Preferably, the micro-fluidic chip cleaning agent comprises the following components in parts by mass:
preferably, the pH of the ethylenediaminetetraacetic acid is 7.5-8.5.
Preferably, the pH value of the micro-fluidic chip cleaning agent is 7-8.
Preferably, the cleaning agent is an aqueous agent.
In a third aspect of the invention, a method for cleaning a microfluidic chip is provided, and the microfluidic chip is cleaned by using the cleaning agent for the microfluidic chip.
Preferably, the method comprises the following steps:
2.1) respectively adding 10-20ul of MilliQ water into the holes of the microfluidic chip, and uniformly mixing and oscillating at a low speed;
2.2) holding two sides of the chip, and downwards knocking the microfluidic chip until all the solution in the microfluidic chip hole flows out;
2.3) adding the micro-fluidic chip cleaning agent into the micro-fluidic chip holes respectively, performing vortex oscillation, and removing the solution in the micro-fluidic chip holes by adopting the method in the step 2.2) to obtain the clean micro-fluidic chip.
The chips are knocked by moderate manual force, and the residual liquid in the chip lane is removed. Thus, devices such as a pump or a negative pressure device are completely abandoned, and the operation of a laboratory is convenient and easy.
The method can effectively remove the residual glue in the used micro-fluidic chip hole, so that the micro-fluidic chip hole can be reused, and the cost is greatly saved.
Preferably, the method comprises the following steps: and adding the micro-fluidic chip cleaning agent into the periphery of the micro-fluidic chip hole after glue pouring.
The method can remove the redundant glue on the periphery of the glued microfluidic chip hole, so that the electrophoresis result is more accurate.
Preferably, the liquid adding amount of the micro-fluidic chip cleaning agent is 5-20 ul.
Compared with the prior art, the invention has the following advantages or beneficial effects: the micro-fluidic chip cleaning agent provided by the invention is common chemical raw materials, is very cheap, can realize the reutilization of the chip without preparing additional pumps or negative pressure devices and the like in a laboratory, and has the advantages of economical use and strong operability.
Drawings
The invention and its features, aspects and advantages will become more apparent from reading the following detailed description of non-limiting embodiments with reference to the accompanying drawings. Like reference symbols in the various drawings indicate like elements. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the invention.
FIG. 1 is a diagram showing the results of cleaning a chip with a cleaning agent and a cleaning method according to the prior art;
FIG. 2 is a graph of the results of baseline stability testing of the chip after 5 washes with the cleaning agent in the example of the present invention;
FIG. 3 is a graph showing the results of comparative tests before and after cleaning with the cleaning agent in the example of the present invention;
FIG. 4 is a graph showing the results of a repeated cleaning test in an embodiment of the present invention;
FIG. 5 is a graph showing the results of a multi-cleaning test according to an embodiment of the present invention.
Detailed Description
Those skilled in the art will appreciate that variations may be implemented by those skilled in the art in combination with the prior art and the above-described embodiments, and will not be described herein in detail. Such variations do not affect the essence of the present invention and are not described herein.
Examples 1 to 3
The microfluidic chip cleaning agents of examples 1-3 were prepared according to the raw materials and formulations in table 1 as follows:
1. weighing ethylenediaminetetraacetic acid by using a balance, fully dissolving the ethylenediaminetetraacetic acid into 1L of deionized water, and adjusting the pH value to 8.0;
weighing trihydroxymethyl aminomethane by using a balance, adding acetic acid, uniformly mixing, adding the solution obtained in the first step, and finally fixing the volume to 1L;
3. diluting the solution obtained in the second step, and adding oleate according to the volume ratio of 1% to prepare the finished cleaning reagent for later use.
Examples 1 to 3 were subjected to the following cleaning effect test.
[ Baseline stability test of chips after 5 washes with detergent ]
The chip was washed 5 times as follows
1. Adding 10-20ul MilliQ water (>18M omega) into each hole of the used microfluidic chip, uniformly mixing at low speed of 500rpm, and oscillating for 5 minutes;
2. preparing sterile dust-free paper, holding two sides of the chip by a thumb and a forefinger, knocking the chip downwards for 20 times (paying attention to the fact that the fingers do not contact glass on the back of the chip), and ensuring that no solution remains in each hole of the chip;
3. adding 18ul of the cleaning agent in the example 1 into each hole, and removing the solution residue in the chip hole in the step 2 after vortex oscillation for 1 minute/2400 rpm;
4. ensuring to remove residual liquid in chip holes, adding 9ul of glue dye mixture into glue filling holes, performing normal pressure glue filling according to the specification, adding 18ul of the cleaning agent in the embodiment 1 into each hole, removing the residual liquid in each hole according to the step 2, and adding 6ul of the glue dye mixture into each waste liquid hole;
5. add ladder and sample according to the chip vendor's instructions. After vortex oscillation for 1 min/2400 rpm, the program was run plus voltage running electrophoresis;
6. the method of the step 1-5 is continuously adopted to repeatedly carry out the micro-fluidic chip cleaning and the glue running for 4 times;
7. after the 5 th washing, the electrophoresis experiment is carried out according to the method of the step 4-5, and H is used2O as blank sample.
As shown in FIG. 2, after 5 times of cleaning, the baseline stability of the chip is good, and it can be seen that the chip can be reused after being cleaned by the cleaning agent provided by the invention for many times.
[ comparative test before and after washing ]
1. A brand new microfluidic chip was taken and ladder and sample added according to the instructions of the chip supplier. After vortex oscillation for 1 min/2400 rpm, the program was run plus voltage running electrophoresis; and detecting the electrophoresis result;
2. adding 10-20ul MilliQ water (>18M omega) into each hole of the used microfluidic chip, uniformly mixing at low speed of 500rpm, and oscillating for 5 minutes;
3. preparing sterile dust-free paper, holding two sides of the chip by a thumb and a forefinger, knocking the chip downwards for 20 times (paying attention to the fact that the fingers do not contact glass on the back of the chip), and ensuring that no solution remains in each hole of the chip;
4. adding 18ul of the cleaning agent of example 2 into each hole, and removing the solution residue in the chip holes in reference to step 3 after vortex oscillation for 1 minute/2400 rpm;
5. ensuring to remove residual liquid in chip holes, adding 9ul of glue dye mixture into glue filling holes, performing normal pressure glue filling according to the specification, adding 18ul of the cleaning agent in the embodiment 2 into each hole, removing the residual liquid in each hole according to the step 3, and adding 6ul of the glue dye mixture into each waste liquid hole;
6. add ladder and sample according to the chip vendor's instructions. After vortex oscillation for 1 min/2400 rpm, the program was run plus voltage running electrophoresis; and detecting the electrophoresis result.
The results of 2 times of electrophoresis are shown in FIG. 3, and it can be seen that there is little difference in the electrophoresis results of the same sample before and after washing.
[ cleaning effect repeat test ]
2 used chips were taken for the following experiment:
1. adding 10-20ul MilliQ water (>18M omega) into each hole of the used microfluidic chip, uniformly mixing at low speed of 500rpm, and oscillating for 5 minutes;
2. preparing sterile dust-free paper, holding two sides of the chip by a thumb and a forefinger, knocking the chip downwards for 20 times (paying attention to the fact that the fingers do not contact glass on the back of the chip), and ensuring that no solution remains in each hole of the chip;
3. adding 18ul of the cleaning agent of example 3 into each hole, and removing the solution residue in the chip holes in the reference step 2 after vortex oscillation for 1 minute/2400 rpm;
4. ensuring to remove residual liquid in chip holes, adding 9ul of glue dye mixture into glue filling holes, performing normal pressure glue filling according to the specification, adding 18ul of the cleaning agent in the embodiment 3 into each hole, removing the residual liquid in each hole according to the step 2, and adding 6ul of the glue dye mixture into each waste liquid hole;
5. add ladder and sample according to the chip vendor's instructions. After vortexing for 1 min/2400 rpm, the program was run plus voltage running electrophoresis.
The electrophoresis results of 2 chips are shown in FIG. 4, and it can be seen that the electrophoresis structure of 2 different chips was almost indistinguishable after the cleaning agent of example 3. Experiments prove that the cleaning agent provided by the invention has the stability of the chip cleaning effect.
[ multiple cleaning Effect test ]
1 used chip was taken out, and the following experiment was repeated 5 times:
1. adding 10-20ul MilliQ water (>18M omega) into each hole of the used microfluidic chip, uniformly mixing at low speed of 500rpm, and oscillating for 5 minutes;
2. preparing sterile dust-free paper, holding two sides of the chip by a thumb and a forefinger, knocking the chip downwards for 20 times (paying attention to the fact that the fingers do not contact glass on the back of the chip), and ensuring that no solution remains in each hole of the chip;
3. adding 18ul of the cleaning agent in the example 1 into each hole, and removing the solution residue in the chip hole in the step 2 after vortex oscillation for 1 minute/2400 rpm;
4. ensuring to remove residual liquid in chip holes, adding 9ul of glue dye mixture into glue filling holes, performing normal pressure glue filling according to the specification, adding 18ul of the cleaning agent in the embodiment 1 into each hole, removing the residual liquid in each hole according to the step 2, and adding 6ul of the glue dye mixture into each waste liquid hole;
5. add ladder and sample according to the chip vendor's instructions. After vortexing for 1 min/2400 rpm, the program was run plus voltage running electrophoresis.
The results of 5 times electrophoresis are shown in FIG. 5, and it can be seen that there is little difference in the results of the chip detection samples after 5 times washing. Experiments prove that the chip repeatedly cleaned by the cleaning solution provided by the invention can be repeatedly used.
In conclusion, the chip cleaning agent and the method for cleaning the excess glue around the chip hole in the sample adding process can effectively remove the chip residual glue. The combination of the two methods of the cleaning agent for the bit stream control chip provided by the invention can increase the stability of an electrophoresis test, and the flow control chip can be repeatedly utilized for 10 times, thereby greatly saving the cost.
The above description is of the preferred embodiment of the invention. It is to be understood that the invention is not limited to the particular embodiments described above, in that devices and structures not described in detail are understood to be implemented in a manner common in the art; those skilled in the art can make many possible variations and modifications to the disclosed embodiments, or modify equivalent embodiments to equivalent variations, without departing from the spirit of the invention, using the methods and techniques disclosed above. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention are still within the scope of the protection of the technical solution of the present invention, unless the contents of the technical solution of the present invention are departed.
Claims (6)
1. A method for preparing a micro-fluidic chip cleaning agent is characterized by comprising the following steps:
1.1) preparing an ethylene diamine tetraacetic acid deionized water solution, and adjusting the pH value to 7.5-8.5;
1.2) adding the trihydroxymethyl aminomethane into acetic acid to form a mixed solution;
1.3) adding the ethylenediamine tetraacetic acid deionized water solution prepared in the step 1.1) into the mixed solution obtained in the step 1.2);
1.4) adding oleate into the mixed solution obtained in the step 1.3), and adjusting the pH value to 7-8 to obtain the micro-fluidic chip cleaning agent;
the micro-fluidic chip cleaning agent comprises the following components in parts by mass:
2. a micro-fluidic chip cleaning agent prepared by the method for preparing a micro-fluidic chip cleaning agent according to claim 1.
3. A method for cleaning a microfluidic chip, which is characterized in that the microfluidic chip cleaning agent as claimed in claim 2 is used for cleaning.
4. A method according to claim 3, characterized by the steps of:
2.1) respectively adding 10-20uL of Milli-Q water into the holes of the microfluidic chip, and uniformly mixing and oscillating at low speed;
2.2) holding two sides of the chip, and downwards knocking the microfluidic chip until all the solution in the microfluidic chip hole flows out;
2.3) adding the micro-fluidic chip cleaning agent in claim 2 into the micro-fluidic chip holes respectively, performing vortex oscillation, and removing the solution in the micro-fluidic chip holes by adopting the method in the step 2.2) to obtain the clean micro-fluidic chip.
5. The method of claim 4, further comprising the steps of: adding the micro-fluidic chip cleaning agent of claim 2 to the periphery of the micro-fluidic chip hole after glue pouring.
6. The method according to claim 4 or 5, wherein the amount of the micro-fluidic chip cleaning agent added is 5-20 uL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010887325.1A CN112048401B (en) | 2020-08-28 | 2020-08-28 | Micro-fluidic chip cleaning agent and method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010887325.1A CN112048401B (en) | 2020-08-28 | 2020-08-28 | Micro-fluidic chip cleaning agent and method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112048401A CN112048401A (en) | 2020-12-08 |
| CN112048401B true CN112048401B (en) | 2022-05-17 |
Family
ID=73608091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010887325.1A Expired - Fee Related CN112048401B (en) | 2020-08-28 | 2020-08-28 | Micro-fluidic chip cleaning agent and method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112048401B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115044417A (en) * | 2022-05-11 | 2022-09-13 | 苏州莱博睿思生物科技有限公司 | Cleaning fluid, preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349272A (en) * | 2015-11-17 | 2016-02-24 | 港龙生物技术（深圳）有限公司 | Cleaning agent used for microscale liquid-transfering needle, and preparation method and usage method thereof |
| CN106867698A (en) * | 2016-12-27 | 2017-06-20 | 泰州欣康基因数码科技有限公司 | A kind of biochip cleaning fluid |
| CN106943880A (en) * | 2017-01-17 | 2017-07-14 | 南京中材水务股份有限公司 | A kind of cleaning agents of membrane, its preparation method and its application |
| CN108676629A (en) * | 2018-06-13 | 2018-10-19 | 北京日光旭升精细化工技术研究所 | Remove bloodstain cleaning agent and preparation method thereof |
| CN108913376A (en) * | 2018-05-30 | 2018-11-30 | 武汉华大医学检验所有限公司 | A kind of reagent and its preparation method and application for sequence testing chip cleaning |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100523212B1 (en) * | 2003-01-04 | 2005-10-24 | 한국과학기술원 | A Protein Chip for Analyzing Interaction Between Protein and Substrate Peptide Therefor |
| CN101000339B (en) * | 2007-01-22 | 2011-05-11 | 上海浩源生物科技有限公司 | Method for reusing microflow chip |
| JP5642361B2 (en) * | 2008-07-03 | 2014-12-17 | ジョンソン・アンド・ジョンソン・アーベー | Methods for analysis of circulating antibodies |
| CN101363838A (en) * | 2008-09-02 | 2009-02-11 | 浙江大学 | Method for detecting blood serum tumor markers |
| CN101363837A (en) * | 2008-09-02 | 2009-02-11 | 浙江大学 | Method for estimating curative effect of pancreatic cancer chemotherapy medicine |
| TWI509074B (en) * | 2012-11-28 | 2015-11-21 | Fooyin University Hospital | Enzyme Gene Chip Detection Reagent and Its Detection |
-
2020
- 2020-08-28 CN CN202010887325.1A patent/CN112048401B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349272A (en) * | 2015-11-17 | 2016-02-24 | 港龙生物技术（深圳）有限公司 | Cleaning agent used for microscale liquid-transfering needle, and preparation method and usage method thereof |
| CN106867698A (en) * | 2016-12-27 | 2017-06-20 | 泰州欣康基因数码科技有限公司 | A kind of biochip cleaning fluid |
| CN106943880A (en) * | 2017-01-17 | 2017-07-14 | 南京中材水务股份有限公司 | A kind of cleaning agents of membrane, its preparation method and its application |
| CN108913376A (en) * | 2018-05-30 | 2018-11-30 | 武汉华大医学检验所有限公司 | A kind of reagent and its preparation method and application for sequence testing chip cleaning |
| CN108676629A (en) * | 2018-06-13 | 2018-10-19 | 北京日光旭升精细化工技术研究所 | Remove bloodstain cleaning agent and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112048401A (en) | 2020-12-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112048401B (en) | Micro-fluidic chip cleaning agent and method thereof | |
| US20050282293A1 (en) | System for delivering a diluted solution | |
| WO2007035414A2 (en) | Methods and systems for fluid delivery | |
| JP2011513737A (en) | Apparatus and method for cleaning fluid processing probes | |
| Wommack et al. | Methods for the isolation of viruses from environmental samples | |
| CN112058325B (en) | Ultrasonic phased array microfluidic detection device and method based on immunomagnetic bead technology | |
| WO2014048263A1 (en) | Method for pressure extraction of nucleic acids and device thereof | |
| US8969098B2 (en) | Methods and systems providing reagent mixing | |
| JP2006078475A (en) | Reactor and manufacturing method therefor | |
| JP3343038B2 (en) | Dispensing method in automatic sample analyzer | |
| CN102297795B (en) | Computer-controlled automatic liquid micro-extraction device | |
| JP2008241250A (en) | Stirring rod using specifically binding reaction, and analyzing method and device using stirring rod thereof | |
| Kerrouche et al. | Megasonic sonication for cost-effective and automatable elution of Cryptosporidium from filters and membranes | |
| JP4098531B2 (en) | Cell cleaning method for biochemical analyzer | |
| CN101539583A (en) | Automatic analyzer | |
| CN209741157U (en) | Portable microbead washing and recovering device and single cell sequencing sample processing system | |
| CN107091809A (en) | A kind of Silver nanorod array surface strengthens the cleaning method of Raman scattering substrate | |
| CN106916812A (en) | A kind of paramagnetic particle method Ago-Gel DNA extraction kit and its extracting method | |
| CN211652912U (en) | Full-automatic biochemical analyzer | |
| JP2012047457A (en) | Cleaning method of micro fluid device and cleaning liquid | |
| CN101000339B (en) | Method for reusing microflow chip | |
| CN110527346A (en) | Overflow glue remover and preparation method and application thereof | |
| CN114871210B (en) | Cleaning method of sample analyzer, sample analyzer and control device thereof | |
| CN105219836A (en) | The active aldehyde group modified substrate of a kind of microarray | |
| JP3952718B2 (en) | Method of cleaning the analyzer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220517 |