CN112029641A - Fermentation device - Google Patents

Fermentation device Download PDF

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Publication number
CN112029641A
CN112029641A CN202010944826.9A CN202010944826A CN112029641A CN 112029641 A CN112029641 A CN 112029641A CN 202010944826 A CN202010944826 A CN 202010944826A CN 112029641 A CN112029641 A CN 112029641A
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culture tank
pipeline
culture
temperature
valve
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Pending
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CN202010944826.9A
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Inventor
涂桂洪
潘忠林
罗雄生
谭志康
林佰开
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Guangzhou Jinyinyuan Biotechnology Co ltd
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Guangzhou Jinyinyuan Biotechnology Co ltd
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Priority to CN202010944826.9A priority Critical patent/CN112029641A/en
Publication of CN112029641A publication Critical patent/CN112029641A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26Conditioning fluids entering or exiting the reaction vessel
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/02Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/16Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature by recirculation of culture medium at controlled temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls

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  • Health & Medical Sciences (AREA)
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Abstract

The invention discloses a fermentation device, which comprises a first culture tank and a second culture tank, wherein the temperature of the first culture tank is higher than that of the second culture tank, a heating pipeline and a cooling pipeline are arranged between the first culture tank and the second culture tank, a first oxygen-increasing air inlet valve is arranged on the heating pipeline, a second oxygen-increasing air inlet valve is arranged on the cooling pipeline, a culture solution in the first culture tank can enter the second culture tank through the cooling pipeline, and a culture solution in the second culture tank can enter the first culture tank through the heating pipeline, so that the reaction speed of microorganisms can be effectively increased, the speed of microbial metabolites is increased, and the yield of the metabolites is increased.

Description

Fermentation device
Technical Field
The invention belongs to the technical field of fermentation, and particularly relates to a fermentation device.
Background
The high-temperature bacteria are extreme microorganisms capable of growing at the temperature of over 45 ℃, small molecular peptides, protease and the like generated by the high-temperature bacteria have good heat resistance, but the oxygen solubility of the high-temperature bacteria is low in the high-temperature environment, the growth speed of the bacteria is slow, and the metabolic products are generated slowly, so that the fermentation yield is low; the genetic engineering bacteria for inducing secretion products at low temperature are adopted, the cell division speed is low, the growth speed of the bacteria is slow, the total amount of the bacteria is not high, and the fermentation yield is low.
Disclosure of Invention
The present invention is directed to solve at least one of the problems of the prior art, and to provide a fermentation apparatus capable of increasing the production rate of microbial metabolites and increasing the fermentation yield.
The technical scheme adopted by the invention for solving the technical problems is as follows: the utility model provides a fermenting installation, including first cultivation jar and second cultivation jar, the temperature of first cultivation jar is higher than the temperature of second cultivation jar, first cultivation jar with be equipped with intensification pipeline and cooling pipeline between the second cultivation jar, be equipped with first oxygenation admission valve on the intensification pipeline, be equipped with second oxygenation admission valve on the cooling pipeline, culture solution in the first cultivation jar can pass through the cooling pipeline gets into the second cultivation jar, culture solution in the second cultivation jar can pass through the intensification pipeline gets into the first cultivation jar.
As a further improvement of the technical scheme of the invention, a first jacket is installed on the warming pipeline, and a first water inlet valve and a first water outlet valve are respectively arranged at two ends of the first jacket.
As a further improvement of the technical scheme of the invention, a second jacket is arranged on the cooling pipeline, and a second water inlet valve and a second water outlet valve are respectively arranged at two ends of the second jacket.
Further as an improvement of the technical scheme of the invention, stirring devices are arranged on the first culture tank and the second culture tank.
Further as an improvement of the technical scheme of the invention, defoaming electrodes are arranged on the first culture tank and the second culture tank respectively.
As a further improvement of the technical scheme of the invention, a first one-way valve is arranged in the cooling pipeline, and an outlet of the first one-way valve faces the second culture tank.
As a further improvement of the technical scheme of the invention, a second one-way valve is arranged in the warming pipeline, and an outlet of the second one-way valve faces to the first culture tank.
As a further improvement of the technical scheme of the invention, the first culture tank and the second culture tank are both provided with heat preservation devices.
As a further improvement of the technical scheme of the invention, a first suction pump is arranged in the temperature rising pipeline.
As a further improvement of the technical scheme of the invention, a second suction pump is arranged in the cooling pipeline.
One of the above technical solutions has at least one of the following advantages or beneficial effects: cultivate in first culture tank when cultivateing the high temperature bacterial, the temperature can reduce when the cooling tube is flowed through to the culture solution in the first culture tank, open second oxygenation admission valve and let in aseptic oxygen to cooling tube, make the dissolved oxygen volume of culture solution increase, culture solution after the cooling gets into the second culture tank and cultivates, further improve the intracellular oxygen content of culture solution, cultivate in the second culture tank and accomplish the back, with the culture solution in the second culture tank through the pipeline intensification backward flow to first culture tank, continue to carry out high temperature cultivation, make the microorganism contain more oxygen like this, can effectively promote the reaction rate of microorganism, make the speed of microorganism metabolite accelerate, improve fermentation output.
Drawings
The invention will be further described with reference to the accompanying drawings in which:
FIG. 1 is a schematic structural diagram of one embodiment of the present invention;
fig. 2 is a detailed illustration of the embodiment shown in fig. 1.
Detailed Description
Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.
In the present invention, if directions (up, down, left, right, front, and rear) are described, it is only for convenience of describing the technical solution of the present invention, and it is not intended or implied that the technical features referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, it is not to be construed as limiting the present invention.
In the invention, the meaning of "a plurality" is one or more, the meaning of "a plurality" is more than two, and the terms of "more than", "less than", "more than" and the like are understood to exclude the number; the terms "above", "below", "within" and the like are understood to include the instant numbers. In the description of the present invention, if there is description of "first" and "second" only for the purpose of distinguishing technical features, it is not to be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features or implicitly indicating the precedence of the indicated technical features.
In the present invention, unless otherwise specifically limited, the terms "disposed," "mounted," "connected," and the like are to be understood in a broad sense, and for example, may be directly connected or indirectly connected through an intermediate; can be fixedly connected, can also be detachably connected and can also be integrally formed; may be mechanically coupled, may be electrically coupled or may be capable of communicating with each other; either as communication within the two elements or as an interactive relationship of the two elements. The specific meaning of the above-mentioned words in the present invention can be reasonably determined by those skilled in the art in combination with the detailed contents of the technical solutions.
Referring to fig. 1 and 2, an embodiment of the present invention provides a fermentation apparatus, including a first culture tank 1 and a second culture tank 2, where the temperature of the first culture tank 1 is higher than that of the second culture tank 2, a temperature rising pipeline 4 and a temperature reducing pipeline 3 are disposed between the first culture tank 1 and the second culture tank 2, the temperature rising pipeline 4 is provided with a first oxygen increasing inlet valve 41, the temperature reducing pipeline 3 is provided with a second oxygen increasing inlet valve 31, a culture solution in the first culture tank 1 can enter the second culture tank 2 through the temperature reducing pipeline 3, and a culture solution in the second culture tank 2 can enter the first culture tank 1 through the temperature rising pipeline 4.
Wherein the first culture tank 1 is a high temperature culture tank, the culture temperature is over 43 ℃, and the second culture tank 2 is a low temperature culture tank, the culture temperature is below 30 ℃.
The bottom at first culture tank 1 and second culture tank 2 is connected to cooling pipe 3, and intensification pipeline 4 is located cooling pipe 3 top, and the one end intercommunication first culture tank 1 of intensification pipeline 4, and jar 2 is cultivateed and stretch into the bottom of second culture tank 2 to the other end intercommunication second of intensification pipeline 4.
The high-temperature bacteria need to synthesize products in a high-temperature environment, the dissolved oxygen in the culture solution is low at high temperature, and the dissolved oxygen in the culture solution is high at low temperature or normal temperature. Cultivate in first culture tank 1 when cultivateing the high temperature bacterial, the temperature can reduce when the culture solution in first culture tank 1 flows through cooling tube 3, open second oxygenation admission valve 31 and let in aseptic oxygen to cooling tube 3, make the dissolved oxygen volume of culture solution increase, culture solution after the cooling gets into and cultivates in second culture tank 2, further improve the intracellular oxygen content of culture solution, cultivate in second culture tank 2 and accomplish the back, with the culture solution in second culture tank 2 through warming up 4 intensification backward flows to first culture tank 1 of pipeline, continue to carry out high temperature cultivation, make the microorganism contain more oxygen like this, can effectively promote the reaction rate of microorganism, make fermentation output increase.
The genetic engineering bacteria for inducing secretion products at low temperature are adopted, the cell division speed is slow under the low-temperature environment, the growth and separation speed of the bacteria is slow, the total amount of the bacteria is not high, the bacteria are cultured in the second culture tank 2 when low-temperature strains are cultured, the temperature of the culture solution in the second culture tank 2 is increased when the culture solution flows through the heating pipeline 4, meanwhile, the first oxygen-increasing air inlet valve 41 on the heating pipeline 4 is opened to introduce sterile oxygen, so that the dissolved oxygen of the culture solution is increased, the heated culture solution enters the first culture tank 1, the intracellular dissolved oxygen of the culture solution is further increased, the number of bacteria division in the culture tank 1 is increased, the growth speed is accelerated, after the culture in the first culture tank 1 is finished, the culture solution is cooled through the cooling pipeline 3 and then flows back to the second culture tank 2 for low-temperature culture, so that the number of microorganisms for low-temperature culture in the second culture tank 2 is increased, the yield of fermentation product is increased.
The first jacket 42 is installed on the warming pipeline 4, a first water inlet valve 421 and a first water outlet valve 422 are respectively arranged at two ends of the first jacket 42, the first water inlet valve 421 is located below the first water outlet valve 422, specifically, water vapor or hot water enters the first jacket 42 through the first water inlet valve 421, heat exchange is achieved in the first jacket 42, the temperature of the warming pipeline 4 is increased, so that the culture solution flowing through the warming pipeline 4 is heated and warmed, and the water vapor or hot water after heat exchange flows out through the first water outlet valve 422.
Of course, heating may be performed by a method such as electric heating, in addition to heat exchange using a heat exchange medium such as hot water or steam.
The cooling pipeline 3 is provided with a second jacket 32, two ends of the second jacket 32 are respectively provided with a second water inlet valve 321 and a second water outlet valve 322, the second water inlet valve 321 is positioned below the second water outlet valve 322, specifically, cold water enters the second jacket 32 through the second water inlet valve 321, heat exchange is realized in the second jacket 32 to cool the culture solution flowing through the cooling pipeline 3, and the cold water after heat exchange flows out from the second water outlet valve 322.
In some embodiments, the stirring devices 5 are installed on the first culture tank 1 and the second culture tank 2, and the stirring devices 5 increase the reaction rate of the culture solution, thereby further increasing the intracellular dissolved oxygen amount of the culture solution.
In some embodiments, defoaming electrodes 6 are installed on both the first culture tank 1 and the second culture tank 2 to remove foam generated during fermentation and improve the reaction efficiency of the culture solution.
Be equipped with first check valve 33 on the cooling tube way 3, jar 2 is cultivateed to the export of first check valve 33 towards the second, makes the culture solution of the first culture tank 1 of outflow can only one-wayly get into in the second culture tank 2 like this, avoids appearing the backward flow phenomenon, is equipped with second check valve 43 in the pipeline 4 that heaies up, and the export of second check valve 43 is towards first culture tank 1, and the culture solution of the second culture tank 2 of outflow can only one-wayly get into in the first culture tank 1.
In some embodiments, the first culture tank 1 and the second culture tank 2 are both provided with an incubation device 7 to prevent the temperature of the first culture tank 1 and the second culture tank 2 from being affected by the external environment.
In some embodiments, the first suction pump 44 is provided in the warming conduit 4, and the first suction pump 44 provides power for the culture solution to flow from the second culture tank 2 into the first culture tank 1, and the second suction pump 34 is provided in the warming conduit 3, and the second suction pump 34 provides power for the culture solution to flow from the first culture tank 1 into the second culture tank 2.
In addition, all be equipped with manometer 11, temperature electrode 12, PH electrode 13, air inlet unit 14 and waste gas valve 15 on first culture tank 1 and the second culture tank 2, wherein manometer 11 is used for detecting jar internal pressure, and temperature electrode 12 is used for monitoring jar internal temperature, and PH electrode 13 is used for detecting the PH value of culture solution, and air inlet unit 14 provides oxygen for jar interior microbial reaction, and waste gas valve 15 is used for discharging the waste gas in the jar.
The invention is not limited to the above embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the invention, and such equivalent modifications or substitutions are included in the scope defined by the claims of the present application.

Claims (10)

1. A fermentation device, characterized in that: including first cultivation jar and second cultivation jar, the temperature of first cultivation jar is higher than the temperature of second cultivation jar, first cultivation jar with be equipped with intensification pipeline and cooling pipeline between the second cultivation jar, be equipped with first oxygenation admission valve on the intensification pipeline, be equipped with second oxygenation admission valve on the cooling pipeline, culture solution in the first cultivation jar can pass through cooling pipeline gets into the second cultivation jar, culture solution in the second cultivation jar can pass through the intensification pipeline gets into first cultivation jar.
2. The fermentation apparatus according to claim 1, wherein: the heating pipeline is provided with a first jacket, and two ends of the first jacket are respectively provided with a first water inlet valve and a first water outlet valve.
3. The fermentation apparatus according to claim 1, wherein: and a second jacket is arranged on the cooling pipeline, and a second water inlet valve and a second water outlet valve are respectively arranged at two ends of the second jacket.
4. The fermentation apparatus according to claim 1, wherein: and stirring devices are arranged on the first culture tank and the second culture tank.
5. The fermentation apparatus according to claim 1, wherein: defoaming electrodes are arranged on the first culture tank and the second culture tank.
6. The fermentation apparatus according to claim 1, wherein: a first one-way valve is arranged in the cooling pipeline, and an outlet of the first one-way valve faces the second culture tank.
7. The fermentation apparatus according to claim 1, wherein: and a second one-way valve is arranged in the warming pipeline, and the outlet of the second one-way valve faces to the first culture tank.
8. The fermentation apparatus according to claim 1, wherein: and the first culture tank and the second culture tank are both provided with heat preservation devices.
9. The fermentation apparatus according to claim 1, wherein: and a first suction pump is arranged in the temperature rising pipeline.
10. The fermentation apparatus according to claim 1, wherein: and a second suction pump is arranged in the cooling pipeline.
CN202010944826.9A 2020-09-10 2020-09-10 Fermentation device Pending CN112029641A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010944826.9A CN112029641A (en) 2020-09-10 2020-09-10 Fermentation device

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Application Number Priority Date Filing Date Title
CN202010944826.9A CN112029641A (en) 2020-09-10 2020-09-10 Fermentation device

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CN112029641A true CN112029641A (en) 2020-12-04

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CN202010944826.9A Pending CN112029641A (en) 2020-09-10 2020-09-10 Fermentation device

Country Status (1)

Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113583859A (en) * 2021-08-27 2021-11-02 重庆赛诺生物药业股份有限公司 Oxygen-resistant bifidobacterium fermentation equipment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113583859A (en) * 2021-08-27 2021-11-02 重庆赛诺生物药业股份有限公司 Oxygen-resistant bifidobacterium fermentation equipment

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