CN112023707A - Shadowless film counterdyeing method - Google Patents
Shadowless film counterdyeing method Download PDFInfo
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- CN112023707A CN112023707A CN202010772923.4A CN202010772923A CN112023707A CN 112023707 A CN112023707 A CN 112023707A CN 202010772923 A CN202010772923 A CN 202010772923A CN 112023707 A CN112023707 A CN 112023707A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
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Abstract
The invention discloses a shadowless film counterdyeing method, which comprises the following steps: uncovering the original specimen cover glass with tweezers, dripping a photocatalyst liquid to the periphery of the specimen, and adsorbing the shadowless film after the specimen is dissociated from the glass slide; then, mounting a new shadowless film interlayer cup with the same specification on a working instrument, and transferring the shadowless film to the bottom of the new interlayer cup; then, dropwise adding a removing liquid, and immediately sucking at negative pressure until the photocatalyst liquid is removed and the shadowless film is restored to the initial state; and finally, re-dyeing the shadowless film. The invention provides a shadowless film counterdyeing method which can well complete counterdyeing and transfer work of a shadowless film.
Description
Technical Field
The invention relates to the field of medical detection, in particular to a shadowless membrane counterstaining method.
Background
Chinese patent application No. 201110462479.7: a detection method using membrane separation and membrane transparent liquid disclose a filtering membrane made of polyvinylidene fluoride, which is changed from a non-transparent state to a transparent state under the action of the membrane transparent liquid, so as to be beneficial to directly observing and counting substances adhered on the filtering membrane. The filtration membrane in the above patent, which can be changed from a non-transparent state to a transparent state, is a shadowless membrane.
The shadowless membrane can be used for conveniently observing and counting substances, but after the company performs summary analysis on the feedback problems in the clinical application process, the company finds that the situation that the staining is poor or cells and bacteria fade after the shadowless membrane is placed for a long time after the staining is poor due to the fact that the thickness of the shadowless membrane is only about 20 micrometers, and the staining is difficult to perform again (the staining is performed again, namely the staining is performed again) when the specimen is required to be checked or rechecked; and the thickness of the shadowless film is only about 20 microns, so that the phenomena of wrinkling, breakage and the like of the shadowless film are easy to occur in the transfer process.
Disclosure of Invention
The invention provides a shadowless film counterdyeing method which can well complete counterdyeing and transfer work of a shadowless film.
In order to realize the purpose, the invention adopts the technical scheme that: the method comprises the following steps:
1) uncovering the original specimen cover glass with tweezers, dripping a photocatalyst liquid to the periphery of the specimen to enable the shadowless film to be in a transparent state, and adsorbing the shadowless film after the specimen is dissociated from the glass slide;
2) installing a new shadowless film interlayer cup with the same specification on a working instrument, and transferring the shadowless film to the bottom of the new interlayer cup;
3) dripping a scavenging solution, immediately sucking at negative pressure, repeating for many times until the photocatalyst liquid is scavenged and the shadowless film is restored to the initial state;
4) the shadowless film is re-dyed.
Further, the scavenging solution is alcohol with the concentration of 75%.
Further, step 1) and step 2) adsorb the shadowless membrane and shift the used adsorption equipment that is, adsorption equipment includes adsorption part and handle portion, and the adsorption part is used for adsorbing the shadowless membrane, and handle portion conveniently grips, adsorption part and shadowless membrane contact surface are the smooth surface, and when the adsorption part leaned on the shadowless membrane, the shadowless membrane can adsorb on the adsorption part because of surface tension, reach the absorption and shift the purpose, the external diameter and the shadowless membrane looks adaptation of adsorption part.
Further, the optical medium liquid is formed by mixing H201 methyl silicone oil and methyl silicone oil, and the volume ratio of the H201 methyl silicone oil to the methyl silicone oil is 30-38: 62 to 70.
The invention has the beneficial effects that: the phenomena of wrinkling, breakage and the like of the shadowless film can be avoided in the process of counterdyeing and transferring of the shadowless film.
Drawings
FIG. 1 is a schematic view of the structure of an adsorption apparatus.
The text labels in the figures are represented as: 1. an adsorption device; 2. an adsorption part; 3. a handle portion.
Detailed Description
The present invention is described in detail below for the purpose of better understanding technical solutions of the present invention by those skilled in the art, and the description of the present invention is only exemplary and explanatory and should not be construed as limiting the scope of the present invention in any way.
The specific scheme of the invention is as follows: the method comprises the following steps:
1) uncovering an original specimen cover glass by using tweezers, dripping a photocatalyst liquid to the periphery of a specimen, holding the handle part 3 of the adsorption device 1 by an operator after the specimen and a glass slide are dissociated, slowly attaching the adsorption part 2 to a shadowless film, adsorbing the shadowless film on the adsorption part under the action of surface tension, and using the photocatalyst liquid as a film transparent liquid disclosed in the application No. CN 201110462479.7;
2) installing a new shadowless membrane interlayer cup with the same specification on a working instrument, transferring the shadowless membrane to the bottom of the new interlayer cup, and using tweezers and the like for assistance when the shadowless membrane is separated from the adsorption part;
3) dripping the scavenging solution, and immediately performing negative pressure suction by using an MS-5 automatic pelleter, wherein the actions are repeated for many times until the photocatalyst liquid is removed and the shadowless film is restored to the initial state;
4) the shadowless film is re-dyed.
Preferably, the scavenging solution is 75% alcohol, and 2-3ml is added dropwise each time.
Preferably, the photocatalyst liquid is prepared by mixing H201 methyl silicone oil and methyl silicone oil, and the volume ratio of the H201 methyl silicone oil to the methyl silicone oil is 30-38: 62 to 70.
The above detailed description of the method for the double-dyeing of the shadowless film provided by the invention, and the principle and the embodiment of the invention are explained herein, the above description of the embodiment is only used to help understanding the method of the invention and the core idea thereof; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (4)
1. A shadowless film counterdyeing method is characterized by comprising the following steps:
uncovering the original specimen cover glass with tweezers, dripping a photocatalyst liquid to the periphery of the specimen, and adsorbing the shadowless film after the specimen is dissociated from the glass slide;
installing a new shadowless film interlayer cup with the same specification on a working instrument, and transferring the shadowless film to the bottom of the new interlayer cup;
dripping a scavenging solution, and immediately sucking at negative pressure until the photocatalyst solution is scavenged and the shadowless film is restored to the initial state;
the shadowless film is re-dyed.
2. The method according to claim 1, wherein the removing solution is 75% alcohol.
3. The method for the counterdyeing of the shadowless film according to claim 1, wherein the shadowless film is adsorbed and transferred by the adsorption device (1) in the step 1) and the step 2), the adsorption device (1) comprises an adsorption part (2) contacted with the shadowless film and a handle part (3), and the contact surface of the adsorption part (2) and the shadowless film is a smooth surface.
4. The method for counterdyeing the shadowless film according to claim 1, wherein the photocatalyst liquid is prepared by mixing H201 methyl silicone oil and methyl silicone oil, and the volume ratio of the H201 methyl silicone oil to the methyl silicone oil is 30-38: 62 to 70.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010772923.4A CN112023707A (en) | 2020-08-04 | 2020-08-04 | Shadowless film counterdyeing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010772923.4A CN112023707A (en) | 2020-08-04 | 2020-08-04 | Shadowless film counterdyeing method |
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| CN112023707A true CN112023707A (en) | 2020-12-04 |
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| CN202010772923.4A Pending CN112023707A (en) | 2020-08-04 | 2020-08-04 | Shadowless film counterdyeing method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
| CN106198158A (en) * | 2016-08-08 | 2016-12-07 | 湖南省天骑医学新技术股份有限公司 | A kind of microporous filter membrane film-making dyeing detection automatic negative-pressure control method |
| CN106226896A (en) * | 2016-08-08 | 2016-12-14 | 湖南省天骑医学新技术股份有限公司 | A kind of can be with the microscope mirror oil compound method of transparent microporous filter membrane |
| CN106769327A (en) * | 2017-01-23 | 2017-05-31 | 湖南省天骑医学新技术股份有限公司 | A kind of Double-layered cup and the method for inspection for collecting Helminthic Eggs |
| CN108982179A (en) * | 2018-07-25 | 2018-12-11 | 湖南省天骑医学新技术股份有限公司 | A kind of method and apparatus of fast transfer miillpore filter |
-
2020
- 2020-08-04 CN CN202010772923.4A patent/CN112023707A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102042924A (en) * | 2010-10-30 | 2011-05-04 | 湖南省天骑医学新技术有限公司 | Dyeing and flaking method for cells or bacteria |
| CN106198158A (en) * | 2016-08-08 | 2016-12-07 | 湖南省天骑医学新技术股份有限公司 | A kind of microporous filter membrane film-making dyeing detection automatic negative-pressure control method |
| CN106226896A (en) * | 2016-08-08 | 2016-12-14 | 湖南省天骑医学新技术股份有限公司 | A kind of can be with the microscope mirror oil compound method of transparent microporous filter membrane |
| CN106769327A (en) * | 2017-01-23 | 2017-05-31 | 湖南省天骑医学新技术股份有限公司 | A kind of Double-layered cup and the method for inspection for collecting Helminthic Eggs |
| CN108982179A (en) * | 2018-07-25 | 2018-12-11 | 湖南省天骑医学新技术股份有限公司 | A kind of method and apparatus of fast transfer miillpore filter |
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Application publication date: 20201204 |
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