CN112022224A - Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology - Google Patents
Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology Download PDFInfo
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- CN112022224A CN112022224A CN202011052412.1A CN202011052412A CN112022224A CN 112022224 A CN112022224 A CN 112022224A CN 202011052412 A CN202011052412 A CN 202011052412A CN 112022224 A CN112022224 A CN 112022224A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
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Abstract
The invention discloses a virus collecting tube for a new coronavirus nucleic acid mixed collection detection technology, which comprises a collecting tube body and a tube cap, wherein the tube cap is detachably connected to the collecting tube body; the collecting tube is characterized in that an auxiliary pharynx swab breaking piece is connected to the inner concave cylinder and formed by connecting a plurality of pharynx swab breaking ribs, and virus preserving fluid is arranged in the collecting tube body. The invention utilizes the annular space formed between the concave cylinder arranged in the collecting tube body and the tube wall of the collecting tube body to ensure that enough space is provided for placing 10 pharyngeal swab heads; the collection tube is arranged on the high-frequency vortex device, so that 10 pharyngeal swab heads vibrate in the collection tube and rotate around the concave cylinder at the same time, and the epithelial cells on the pharyngeal swab heads can be fully eluted into the collection tube preservation solution.
Description
Technical Field
The invention belongs to the technical field of medical appliances, and particularly relates to a virus collection tube for a novel coronavirus nucleic acid mixed collection detection technology.
Background
Throat swab collection is the main means for collecting new coronavirus nucleic acid detection specimens at present. In the prior art, a single collected pharyngeal swab or a plurality of collected pharyngeal swabs are put into a virus collecting tube, a tube cover is screwed down and marked, and then the virus collecting tube is sent to a laboratory for subsequent inspection.
The existing method for collecting the nucleic acid detection specimen of the new coronavirus mainly comprises a single collection mode and a mixed collection mode. The single extraction is not suitable for the detection of the new coronavirus nucleic acid in large-scale population because of low detection efficiency and high cost; and (3) mixed collection, wherein a 10-in-1 mixed collection technology is the most effective specimen collection mode for the detection of the new coronavirus nucleic acid of large-scale crowds. However, the space structure arranged in the 10 in 1 virus collecting tube used by the current 10 in 1 mixed collecting technology is unreasonable and small, and enough gaps among 10 swabs cannot be ensured; therefore, when the collection tube is subsequently processed by the high-frequency vortex device, the vibration amplitude of the 10 throat swabs in the collection tube is limited, and the vortex time needs to be prolonged for effectively extracting the sample nucleic acid, which causes certain obstruction to the sample nucleic acid extraction process.
Disclosure of Invention
Aiming at the problems, the invention makes up the defects of the prior art and provides a virus collection tube of a new coronavirus nucleic acid mixed collection detection technology; the invention improves the efficiency of nucleic acid detection of the new coronavirus of large-scale crowds, ensures the accuracy of the result of nucleic acid detection, reduces the use cost of the virus collection tube to one tenth of that of the original single collection, makes contribution to timely screening out the positive patients of the new coronavirus, and realizes effective control of virus propagation among crowds.
In order to achieve the purpose, the invention adopts the following technical scheme.
The invention relates to a virus collection tube of a novel coronavirus nucleic acid mixed collection detection technology, which comprises a collection tube body and a tube cap, and is characterized in that: the pipe cap is detachably connected to the collecting pipe body, an inwards concave cylinder is arranged in the collecting pipe body, and an annular space for placing a plurality of pharyngeal swab heads is formed between the inwards concave cylinder and the pipe wall of the collecting pipe body; the collecting tube is characterized in that an auxiliary pharynx swab breaking piece is connected to the inner concave cylinder and formed by connecting a plurality of pharynx swab breaking ribs, and virus preserving fluid is arranged in the collecting tube body.
As a preferable scheme of the invention, the pipe cap is provided with internal threads, and the collecting pipe body is provided with external threads matched and connected with the internal threads of the pipe cap.
As another preferable scheme of the invention, a positioning and shifting-stopping sheet structure is arranged at the joint of the tube cap and the collecting tube body, the positioning and shifting-stopping sheet structure comprises a clamping rib arranged on the collecting tube body and a shifting sheet arranged on the tube cap, and the clamping rib is in matched clamping connection with the shifting sheet.
As another preferable scheme of the invention, the tube cap and the collection tube body are made of polypropylene materials, and the collection tube body is transparent.
As another preferable scheme of the invention, an anti-seepage ring and an anti-leakage pad are arranged in the pipe cap.
In another preferred embodiment of the present invention, the auxiliary pharyngeal swab breaking piece is formed by connecting three pharyngeal swab breaking ribs at equal intervals, and each pharyngeal swab breaking rib has a height of 11.0 mm and a length of 3.1 mm.
In another preferred embodiment of the present invention, the outer diameter of the collection tube body is 24.3 mm and the height thereof is 50.7 mm.
As another preferable scheme of the invention, the diameter of the concave cylinder is 10.0 mm, and the height of the concave cylinder is 20.0 mm.
As another preferable scheme of the invention, the outer diameter of the pipe cap is 30.4 mm, and the height of the pipe cap is 13.6 mm.
As another preferable scheme of the invention, the tube wall of the collecting tube body is provided with 6.0ml of liquid height scale marks, and 10 pharyngeal swab heads can be placed in an annular space formed between the concave cylinder and the tube wall of the collecting tube body.
The invention has the beneficial effects.
1. According to the virus collecting tube adopting the novel coronavirus nucleic acid mixed collection detection technology, an annular space is formed between the concave cylinder arranged in the tube body of the collecting tube and the tube wall of the tube body of the collecting tube, so that enough space is ensured for placing 10 pharyngeal swab heads; the collection tube is arranged on the high-frequency vortex device, so that 10 pharyngeal swab heads vibrate in the collection tube and rotate around the concave cylinder at the same time, and the epithelial cells on the pharyngeal swab heads can be fully eluted into the collection tube preservation solution.
2. 6.0ml of virus preservation solution is the minimum volume of 10 oropharyngeal swab heads completely immersed in the preservation solution, so that the minimum dilution of the specimen is realized, and the accuracy of the result is ensured.
3. The auxiliary pharynx swab breaking piece arranged on the concave cylinder is used as an acting point of the pharynx swab head to facilitate the breakage of the pharynx swab, so that the easy breakage is realized, and the subsequent nucleic acid extraction is convenient.
4. The invention improves the efficiency of nucleic acid detection of the new coronavirus of large-scale crowds, ensures the accuracy of the result of nucleic acid detection, reduces the use cost of the virus collection tube to one tenth of that of the original single collection, makes contribution to timely screening out the positive patients of the new coronavirus, and realizes effective control of virus propagation among crowds.
Drawings
In order to make the technical problems, technical solutions and advantageous effects solved by the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and the detailed description. It should be understood that the detailed description and specific examples, while indicating the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
FIG. 1 is a schematic structural diagram of a virus collection tube according to the novel coronavirus nucleic acid mixed collection detection technology of the present invention.
FIG. 2 is a schematic view of the structure of the virus collecting tube in the detection technique of mixed collection of nucleic acid of coronavirus.
FIG. 3 is a schematic structural diagram of a collecting tube body of a virus collecting tube according to the new coronavirus nucleic acid mixed collection detection technology of the present invention.
FIG. 4 is a schematic side view of the collection tube body of the virus collection tube according to the new coronavirus nucleic acid mixed collection detection technique of the present invention.
FIG. 5 is a schematic view of the internal structure of a virus collection tube after containing a eustachian swab head according to the novel coronavirus nucleic acid mixed collection detection technology.
FIG. 6 is a schematic top view of the structure of the collecting tube body of the virus collecting tube according to the new coronavirus nucleic acid mixed collection and detection technique of the present invention.
FIG. 7 is a schematic structural view of a new coronavirus nucleic acid mixed collection detection technique of the present invention, wherein the pharyngeal swab is pre-broken on the collection tube body of the virus collection tube.
FIG. 8 is a schematic top view of a collecting tube body of a virus collecting tube according to the new coronavirus nucleic acid mixed collection detection technology of the present invention, wherein 10 eustachian swab heads are filled in the collecting tube body and arranged in order.
The labels in the figure are: 1 is the collection pipe body, 2 is the pipe cap, 3 is the indent cylinder, 4 is the pharynx swab head, 5 is the annular space, 6 is supplementary pharynx swab rupture piece, 7 is the pharynx swab rupture muscle, 8 is virus preservation liquid, 9 is the internal thread, 10 is the external screw thread, 11 is the card muscle that stops, 12 is the plectrum, 13 is 6.0ml liquid height scale mark, 14 is the pharynx swab.
Detailed Description
The invention discloses a virus collection tube of a new coronavirus nucleic acid mixed collection detection technology, which is shown in the attached drawing and comprises a collection tube body 1 and a tube cap 2, and is characterized in that: the pipe cap 2 is detachably connected to the collecting pipe body 1, an inward concave cylinder 3 is arranged in the collecting pipe body 1, and an annular space 5 for placing a plurality of pharyngeal swab heads 4 is formed between the inward concave cylinder 3 and the pipe wall of the collecting pipe body 1; the inner concave cylinder 5 is connected with an auxiliary pharynx swab breaking piece 6, the auxiliary pharynx swab breaking piece 6 is formed by connecting a plurality of pharynx swab breaking ribs 7, and the collecting tube body 1 is internally provided with virus preserving fluid 8.
The pipe cap 2 is provided with an internal thread 9, and the collecting pipe body 1 is provided with an external thread 10 which is matched and connected with the internal thread of the pipe cap 2; a positioning and shifting-stopping sheet structure is arranged at the joint of the tube cap 2 and the collecting tube body 1, the positioning and shifting-stopping sheet structure comprises a clamping rib 11 arranged on the collecting tube body 1 and a shifting sheet 12 arranged on the tube cap 2, and the clamping rib 11 is in matched clamping connection with the shifting sheet 12; specifically, the pipe cap rotates at the upper end mouth of pipe of gathering the pipe body, and rotatory plectrum to on the pipe cap can send "clickly" sound on contacting with the card stopping muscle on gathering the pipe body, is sealed for screwing promptly.
The pipe cap 2 and the collecting pipe body 1 are made of polypropylene materials, and the collecting pipe body 1 is transparent, so that the visibility is good.
The pipe cap 2 is internally provided with an anti-seepage ring and an anti-leakage pad, so that the virus preservative fluid 8 can be prevented from leaking outwards, and the sealing property of the collection pipe and the safety of medical personnel are ensured.
The auxiliary throat swab breaking piece 6 is formed by connecting three throat swab breaking ribs 7 at equal intervals, and the height and the length of each throat swab breaking rib 7 are respectively 11.0 mm and 3.1 mm; the auxiliary pharyngeal swab break 6 serves as a point of application for the pharyngeal swab head 4 to facilitate breaking of the pharyngeal swab 14.
The outer diameter of the collecting tube body 1 is 24.3 mm, and the height is 50.7 mm; the diameter of the concave cylinder 3 is 10.0 mm, and the height of the concave cylinder is 20.0 mm; the outer diameter of the pipe cap 2 is 30.4 mm, and the height of the pipe cap is 13.6 mm; the tube wall of the collecting tube body 1 is provided with 6.0ml of liquid height scale marks 13, and 10 pharyngeal swab heads 4 can be placed in an annular space 5 formed between the concave cylinder 3 and the tube wall of the collecting tube body 1.
Specifically, the invention adopts guanidinium isothiocyanate with the concentration of 4 mol/L as the virus preservation solution 8, and the color of the virus preservation solution 8 is easily observed and identified, such as: pink, and certain fluidity is maintained, so that the sampling is convenient.
Specifically, the pharyngeal swab 14 matched with the virus collecting tube is a flocked swab, the handle part of the pharyngeal swab 14 is made of non-wood material, the area of the pharyngeal swab head 4 is enough to collect the retropharyngeal wall or the retropharyngeal fornix part of a subject, and the thickness of villus is 0.7 mm; the expansion coefficient of the swab head is moderate, so that the swab is prevented from being crowded due to excessive expansion; the intensity of the flocking cotton is 1.2D, the quality of the specimen is not influenced when the testee feels good, the swab breaking point is arranged at the position which is about 30.0 mm away from the top end of the swab head, and the swab breaking point is matched with the edge of the upper end pipe orifice of the collecting pipe body 1 and is easy to break.
The use method is explained by combining the attached drawings and the technical scheme of the invention: the head of the person to be collected slightly faces down, the mouth is enlarged, and the tongue is heard, the two pharyngeal tonsils are exposed, the person to be collected passes the pharyngeal swab 14 over the tongue root of the person to be collected, the two pharyngeal tonsils are wiped back and forth with little force for at least 3 times, and then the pharyngeal wall is wiped up and down for at least 3 times. After the operation is finished, the pharynx swab head 4 is pressed against the auxiliary pharynx swab breaking piece 6 on the inward concave cylinder 3, the swab breaking point of the pharynx swab 14 is arranged at the edge of the upper end pipe orifice of the collecting pipe body 1, the pharynx swab head 4 falls into virus preserving liquid 8 of the collecting pipe by breaking with little force, the broken swab rod is discarded, the pipe cap 2 is screwed, and the collecting pipe is arranged on a stable test-tube rack. Continuously and sequentially collecting 10 swabs, putting the swabs into the same collecting tube, performing soft action to avoid aerosol generation, and screwing the tube cap 2 to prevent spilling.
It should be understood that the detailed description of the present invention is only for illustrating the present invention and is not limited by the technical solutions described in the embodiments of the present invention, and those skilled in the art should understand that the present invention can be modified or substituted equally to achieve the same technical effects; as long as the use requirements are met, the method is within the protection scope of the invention.
Claims (10)
1. The utility model provides a new coronavirus nucleic acid is thoughtlessly adopted detection technology virus and is gathered pipe, includes and gathers pipe body, pipe cap, its characterized in that: the pipe cap is detachably connected to the collecting pipe body, an inwards concave cylinder is arranged in the collecting pipe body, and an annular space for placing a plurality of pharyngeal swab heads is formed between the inwards concave cylinder and the pipe wall of the collecting pipe body; the collecting tube is characterized in that an auxiliary pharynx swab breaking piece is connected to the inner concave cylinder and formed by connecting a plurality of pharynx swab breaking ribs, and virus preserving fluid is arranged in the collecting tube body.
2. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the pipe cap is provided with an internal thread, and the collecting pipe body is provided with an external thread which is matched and connected with the internal thread of the pipe cap.
3. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the pipe cap and the collecting pipe body are connected through a positioning shifting piece structure, the positioning shifting piece structure comprises a clamping rib arranged on the collecting pipe body and a shifting piece arranged on the pipe cap, and the clamping rib is in matched clamping connection with the shifting piece.
4. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the pipe cap and the collecting pipe body are made of polypropylene materials, and the collecting pipe body is transparent.
5. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the pipe cap is internally provided with an anti-seepage ring and an anti-seepage pad.
6. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the auxiliary pharynx swab breaking piece is formed by connecting three pharynx swab breaking ribs at equal intervals, the height of each pharynx swab breaking rib is 11.0 mm, and the length of each pharynx swab breaking rib is 3.1 mm.
7. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the outer diameter of the collecting tube body is 24.3 mm, and the height of the collecting tube body is 50.7 mm.
8. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the diameter of the concave cylinder is 10.0 mm, and the height of the concave cylinder is 20.0 mm.
9. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the outer diameter of the pipe cap is 30.4 mm, and the height of the pipe cap is 13.6 mm.
10. The virus collection tube for the mixed collection and detection of the novel coronavirus nucleic acid according to claim 1, which is characterized in that: the tube wall of the collecting tube body is provided with 6.0ml of liquid height scale marks, and 10 pharyngeal swab heads can be placed in an annular space formed between the concave cylinder and the tube wall of the collecting tube body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011052412.1A CN112022224A (en) | 2020-09-29 | 2020-09-29 | Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011052412.1A CN112022224A (en) | 2020-09-29 | 2020-09-29 | Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology |
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| Publication Number | Publication Date |
|---|---|
| CN112022224A true CN112022224A (en) | 2020-12-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011052412.1A Withdrawn CN112022224A (en) | 2020-09-29 | 2020-09-29 | Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology |
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| CN (1) | CN112022224A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300907A (en) * | 2020-12-16 | 2021-02-02 | 中国医科大学附属盛京医院 | Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology |
| CN113116396A (en) * | 2021-04-27 | 2021-07-16 | 连云港市第一人民医院 | Integrated throat swab sampling tube |
-
2020
- 2020-09-29 CN CN202011052412.1A patent/CN112022224A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300907A (en) * | 2020-12-16 | 2021-02-02 | 中国医科大学附属盛京医院 | Virus collection tube adopting novel coronavirus nucleic acid mixed collection detection technology |
| CN113116396A (en) * | 2021-04-27 | 2021-07-16 | 连云港市第一人民医院 | Integrated throat swab sampling tube |
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| PB01 | Publication | ||
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Application publication date: 20201204 |