CN1120173C - Antitumor substance extracted from Grifola - Google Patents
Antitumor substance extracted from Grifola Download PDFInfo
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- CN1120173C CN1120173C CN97192894A CN97192894A CN1120173C CN 1120173 C CN1120173 C CN 1120173C CN 97192894 A CN97192894 A CN 97192894A CN 97192894 A CN97192894 A CN 97192894A CN 1120173 C CN1120173 C CN 1120173C
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/375—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Basidiomycetes
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- Natural Medicines & Medicinal Plants (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Alternative & Traditional Medicine (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
An antitumor substance obtained from the mycelia or fruit bodies of hen-of-the-woods (grifola frondosa) by extraction and fractionation and having a potent immunopotentiating activity. The antitumor activity and immunopotentiating power can be enhanced by extracting the mycelia or fruit bodies with hot water and adding alcohol to the extract in a final concentration of 20 to 70 % by volume (low-concentration addition) to remove the suspension or deposits.
Description
Field of the present invention
The present invention relates to have the antitumorigenic substance of high immune-enhancing activity, this material is to extract from mycelium of " Maitake " mushroom (tree flower Pseudomonas) or sporophore and fractional separation obtains.
The technology of the present invention background
Known from the mycelium of setting colored Pseudomonas or sporophore extract by having β-1, the 3-key connects the β-1 of branched glucose, the 6-key connects that the glucose main chain constitutes or by having β-1, the 6-key connects the β-1 of branched glucose, and the polysaccharide that the 3-key connects glucose main chain formation has antitumour activity (referring to the open No.210901/1984 of Japanese Patent LOP).
It also is known comprising the following steps to prepare cancer-resisting substance, and described step is: with hot water to Grifola frondosa, tree flower Pseudomonas resemble ear orchid (Grifola gigantea) (Tonbimai) or Laetiporussulphureus (Masutake) extract; The concentrating under reduced pressure extracting solution precipitates concentrated solution with organic solvent, throw out is dialysed removing low-molecular-weight material, and with lipophilic organic solvent extraction impurity from dialyzate, to remove them.(referring to the open No.16047/1968 of Japanese Patent).
Yet, those methods of describing among open No.210901/1984 of Japanese Patent LOP and the open No.16047/1968 of Japanese Patent, prepare effectively for a large amount of medicament of preparation with from limited resources and not to be well suited for for the heath food, because the purification step of these methods is quite complicated, and contains the material that suppresses immune-enhancing activity in the product of gained.
Of the present invention open
In the case, the inventor to the method for extracting tree flower bacterium and thus the various extracts of method gained carried out deep research.Found that the antitumorigenic substance that obtains to have higher immune-enhancing activity effectively is possible.Be primarily characterized in that, by to the mycelium of setting colored Pseudomonas or sporophore water are carried out heat extract add in the water-soluble extracting liquid that get pure to the volume final concentration be 20-70% (lower concentration interpolation), remove the buoyant material or be attached to material on the wall of container, thereby improve anti-tumor activity and immune-enhancing activity.
That is to say, the present invention relates to have the dextran/protein complex of immune-enhancing activity and comprise the anti-tumor agent comprising salmosin of this mixture that described mixture is got by the following steps preparation as activeconstituents:
(1) water carries out the heat extraction to mycelium or the sporophore of setting colored Pseudomonas, and this tree bacterium can be Grifola frondosa;
(2) adding alcohol to final volume concentration in the water-soluble extracting liquid of gained is 20-70% (lower concentration interpolation), and described extracting solution is left standstill in 1-25 ℃ in container, and removes and swim in the material on the liquid level or in the liquid or be attached to material on the wall of container; And
(3) adding alcohol to final volume concentration in described extracting solution is 80-90% (high density interpolation), described extracting solution is left standstill in 1-25 ℃, and the precipitation of recovery gained, or after (2) step, described extracting solution is concentrated with the generation precipitation, or be concentrated into described extracting solution dried in common mode.
In the present invention, " Maitake " mushroom (Grifola) can be a Grifola frondosa, white porous tree flower bacterium (Grifola albicans Imaz.), tree flower Pseudomonas umbellate pore furgus (Grifola umbellatus), tree flower Pseudomonas resembles ear orchid etc., and these mushrooms can bright mushroom or the form of dried mushroom use, also can or use its form of powder if necessary with their choppings.
Described heat is extracted in 50-135 ℃ and carried out 15 minutes-3 hours.Be rapid extraction, can carry out this treatment step adding to depress in 100 ℃,, under 2 normal atmosphere, in pressure pan, carry out about 30 minutes to 1 hour or 1 hour for example at about 120 ℃.
Used water is distilled water, purified water, ion exchanged water, tap water or the like.The drying tree flower bacterium of every relatively weight part is adopted the doubly water of (preferred 4-10 doubly) volume of about 4-20.If what adopt is fresh tree bacterium, for the tree bacterium of every weight part, adopt the doubly water of (preferred 2-5 doubly) volume of about 2-10.
In (2) step, the alcohol that joins in the extracting solution can be methyl alcohol, ethanol etc.Adding alcohol to final volume concentration in extracting solution is 20-70%.Can adopt water content is the alcohols of 0-50%.After adding alcohol, in 1-25 ℃ leave standstill 1-20 hour after, just have floating substance on liquid level or in the liquid and occur, perhaps have material to be attached on the wall of container, can be by filtering or these materials being removed with whole pipet, screen cloth etc.
Because with floating substance be attached to material on the wall of container and remove the anti-tumor activity that causes extract and the raising of immune-enhancing activity, this step of removing described material is very important.For this step, must add alcohol to final volume concentration be 20-70%, preferably to final concentration be 30-60%.
Adding alcohol to final volume concentration in (2) solution that obtains of step is 80-99%, preferably to final volume concentration be 80-90% (high density interpolation), make solution at 1-25 ℃ then, preferably leave standstill at 1-5 ℃, to be settled out required material, perhaps, the solution of (2) step gained is concentrated with the generation precipitation under heating, or it is concentrated into dried.
The characteristic of the material of the present invention of gained is as follows:
Outward appearance: brown hygroscopic powder.
Solvability: water-soluble, basic solution and methyl-sulphoxide.
Color reaction: positive in anthrone reaction and ninhydrin reaction.
Aqueous solution characteristic: neutral to slightly acidic.
Molecular weight: about 1000000.
Analysis revealed to gained material of the present invention: its main component is dextran and protein.After the column chromatography purifying, the main component of finding the antitumorigenic substance with immune-enhancing activity of gained of the present invention is dextran/protein complex, wherein dextran and proteinic ratio are mainly 80: 20-99: change between 1, this ratio depends on the quality as the tree bacterium of starting raw material, and the condition of extraction and purifying or the like.
Implement best mode of the present invention (1) extracting method
With 5 liters of distilled water the sporophore of 500 exsiccant Grifola frondosa was extracted 60 minutes down at 120 ℃, adding ethanol to final volume concentration in the 950ml of gained aqueous solution part is 60%.When this solution 4 ℃ leave standstill 12 hours after, on liquid level, in the liquid or on wall of container, have the viscous substance of brownish black to generate.Remove these materials with suction pipe.Add ethanol to final volume concentration at least 80% after, solution is left standstill under 4 ℃ low temperature, obtain the dark brown precipitation of 3g to black.The material of gained is all positive in anthrone reaction and ninhydrin reaction.Behind the column chromatography purifying, find that this material is dextran/protein complex, wherein dextran is 96: 4 with proteinic ratio.
Tsk gel GMPW
XLThe result of the gel filtration chromatography check on the post is to find that its molecular weight is about 1000000.When its dextran partly is hydrolyzed and during with the neutral dextran of high performance liquid chromatography qualitative reaction, has only detected glucose.
Check its protein portion, result to show that this protein is made up of L-glutamic acid, aspartic acid, L-Ala, leucine, Methionin, glycine, Isoleucine, Serine, Xie Ansuan, proline(Pro), Threonine, arginine, phenylalanine, tyrosine, Histidine, tryptophane, methionine(Met), halfcystine etc. with automatic amino acid analyser (only tryptophane efficient liquid phase chromatographic analysis).(2) antitumor test:
Just do not add with the material (hereinafter being referred to as substance A) that obtains in above-mentioned (1) step with in the same mode of step (1) respectively and purely be dissolved in the physiological saline to remove the floating substance on the liquid level or in the liquid or to be attached to the dry substance (hereinafter being referred to as substance B) that the step of the material on the wall of container makes to lower concentration (final volume concentration is 20-70%).To each solution of C3H mouse peritoneal injection of having transplanted the MM-46 cancer 10 times, each dosage is 0.1mg/kg, checks it to the effect that tumor growth suppresses with this, and the result is as shown in table 1.
(every group of 15 mouse, ※ t-test: have 5% or lower significant difference).
Measure tumor growth by following formula and suppress (%):
Tumor growth suppresses (%)=[1-(average tumor weight (g) of treatment group/contrast
The average tumor weight (g) of group)] * 100
That group of application of substances A significantly is better than the inhibition effect of application of substances B group to the inhibition effect of tumor growth.
Use after each substances 5 days, gather scavenger cell and killer T cell from the group of control group (only using physiological saline), application of substances A and the group of application of substances B, according to right
3The absorbed dose of H-thymus pyrimidine is surveyed the activity of cellular immunization active cells.The result is as shown in table 2.
Table 2
Cellular immunization active cells activity ( 3H-thymus pyrimidine specific absorption) | ||
Scavenger cell | Killer T cell | |
Control group (using physiological saline) | 100.0 | 100.0 |
The group of application of substances A | 203.5 | 284.5 |
The group of application of substances B | 157.2 | 233.7 |
From The above results as can be seen, substance A shows stronger anti-tumor activity and immune-enhancing activity than substance B.
Industrial applicibility
The above results shows, by being that 20-70% produces the material that suspends in the floating and liquid of liquid level or the material on the attached ground chamber wall is removed owing in the hot water water lift liquid of tree flower bacterium, adding alcohol to final volume concentration, can improve immune-enhancing activity and tumor growth and suppress activity.
Therefore, the invention is characterized in, is not to extract simply beta glucan, but effectively prepares the glucan/protein complex with immune-enhancing activity by simple method from limited resource.
The material that makes according to the present invention is hypotoxicity and high security, and can make health food and oral drug, and it is oral with the form of tablet, capsule, liquid, syrup etc. particularly to can be used as anti-tumor agent comprising salmosin.
Claims (5)
1. dextran/protein complex by the following step preparation:
(1) water carries out the heat extraction to mycelium or the sporophore of setting colored Pseudomonas (Grifola), and it is to carry out under 50-135 ℃ 15 minutes-3 hours that described heat is extracted, and this tree bacterium can be Grifola frondosa (Grifola frondosa);
(2) adding alcohol to final volume concentration in the water-soluble extracting liquid of gained is 20-70%, and described extracting solution is left standstill under 1-25 ℃ in container, removes the material that swims on the liquid level or in the liquid or attached to the material on the wall of container; And
(3) adding alcohol to final volume concentration in described extracting solution is 80-99%, and described extracting solution is left standstill at 1-25 ℃, and reclaims the precipitation that produces.
2. dextran/protein complex by the following step preparation:
(1) water carries out the heat extraction to mycelium or the sporophore of setting colored Pseudomonas, and described heat is extracted in carries out 15 minutes-3 hours under 50-135 ℃, and this tree bacterium can be Grifola frondosa;
(2) adding alcohol to final volume concentration in the water-soluble extracting liquid of gained is 20-70%, and described extracting solution is left standstill under 1-25 ℃ in container, removes to swim in the material on the liquid level or in the liquid or be attached to material on the wall of container; And
(3) described extracting solution is concentrated with the generation precipitation, or be concentrated into described extracting solution dried.
3. according to the dextran/protein complex of claim 1 or 2, wherein dextran and proteinic ratio are 80: 20-99: 1.
4. the anti-tumor agent comprising salmosin that has immune-enhancing activity contains the dextran/protein complex as claimed in claim 1 or 2 as activeconstituents.
5. according to the dextran/protein complex of claim 1 or 2, join wherein that water carries out to the mycelium of setting colored Pseudomonas or sporophore that heat is extracted and water-soluble extracting liquid in alcohol have the final volume concentration of 30-60%.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8051880A JP2859843B2 (en) | 1996-03-08 | 1996-03-08 | Antitumor substance extracted from Maitake |
JP51880/1996 | 1996-03-08 | ||
JP51880/96 | 1996-03-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1213378A CN1213378A (en) | 1999-04-07 |
CN1120173C true CN1120173C (en) | 2003-09-03 |
Family
ID=12899202
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN97192894A Expired - Lifetime CN1120173C (en) | 1996-03-08 | 1997-03-07 | Antitumor substance extracted from Grifola |
Country Status (7)
Country | Link |
---|---|
US (1) | US5854404A (en) |
EP (1) | EP0893449B1 (en) |
JP (1) | JP2859843B2 (en) |
CN (1) | CN1120173C (en) |
AU (1) | AU2232997A (en) |
DE (1) | DE69730420T2 (en) |
WO (1) | WO1997032896A1 (en) |
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JP2001097881A (en) * | 1999-09-28 | 2001-04-10 | Yukiguni Maitake Co Ltd | Cancer prophylaxis agent, and cancer prophylaxis food or feed all derived from grifola frondosa |
JP2001172194A (en) * | 1999-12-17 | 2001-06-26 | Yukiguni Maitake Co Ltd | Nitrogen monoxide (no)-production inducer originating from grifola frondosa (a kind of mushroom) |
CA2425954A1 (en) * | 2000-04-29 | 2002-11-08 | Eric A. Scheinbart | Compositions and methods for treatment of multiple myeloma |
KR100501039B1 (en) * | 2000-08-23 | 2005-07-18 | 한국 한의학 연구원 | Grifola frondosa extracts for controling side effect of cisplatin |
US7507724B2 (en) * | 2001-01-16 | 2009-03-24 | Sloan-Kettering Institute For Cancer Research | Therapy-enhancing glucan |
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AU2002322702A1 (en) * | 2001-07-27 | 2003-02-17 | Tanical Therapeutics, Inc. | Grifola extracts and methods of use thereof |
WO2004092334A2 (en) * | 2003-04-09 | 2004-10-28 | Jarrow Formulas, Inc. | Methods of producing edible fungi containing activated folates and nutritional supplements containing activated folates |
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CA2455655C (en) * | 2003-07-18 | 2011-11-08 | Shirota, Masaki | A glycoprotein with antidiabetic, antihypertensive, antiobesity and antihyperlipidemic effects from grifola frondosa, and a method for preparing same |
JP4842507B2 (en) * | 2003-11-19 | 2011-12-21 | 株式会社雪国まいたけ | Anti-influenza virus activator |
CN1322141C (en) * | 2004-09-22 | 2007-06-20 | 中国食品发酵工业研究院 | Method of preparing huishuhua (grey tree flower) polysaccharide by enzymolysis |
JP2006273835A (en) * | 2005-03-04 | 2006-10-12 | Michishi Tani | Therapeutic agent for malignant tumor and food or beverage containing the same |
JP2007031665A (en) * | 2005-07-29 | 2007-02-08 | Yukiguni Maitake Co Ltd | Glycoprotein extracted from grifola frondosa |
KR100729213B1 (en) * | 2005-07-29 | 2007-06-19 | 주식회사 라이벌코리아 | Exo-biopolymer isolated from submerged mycelial culture of Grifola frondosa increasing immune activity |
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JP2008106018A (en) | 2006-10-27 | 2008-05-08 | Yukiguni Maitake Co Ltd | Substance having anti-influenza virus activity derived from grifola frondosa and its manufacturing method |
JP2008161109A (en) | 2006-12-28 | 2008-07-17 | Yukiguni Maitake Co Ltd | Method for preventing disease of baby pig in weaning period |
JP2008161120A (en) | 2006-12-28 | 2008-07-17 | Yukiguni Maitake Co Ltd | Mother pig rearing method |
JP5153188B2 (en) * | 2007-03-30 | 2013-02-27 | 小林製薬株式会社 | Th1 / Th2 balance improver |
JP2009191009A (en) | 2008-02-14 | 2009-08-27 | Yukiguni Maitake Co Ltd | Low molecular substance derived from grifola frondosa having immunostimulatory activity and antitumor activity |
US20100178279A1 (en) * | 2009-01-15 | 2010-07-15 | Cornell University | Beta-Glucan Enhances Hematopoietic Progenitor Cells Engraftment and Promotes Recovery from Chemotoxicity |
JP2011184306A (en) * | 2010-03-04 | 2011-09-22 | Yukiguni Maitake Co Ltd | Substance derived from grifola spp. mushroom suppressing elevation of postprandial hyperglycemia |
US8597697B2 (en) | 2011-06-24 | 2013-12-03 | Nutragenesis, Llc | Composition of beta-glucan and ashwagandha |
CN103059160B (en) * | 2011-10-20 | 2015-12-02 | 中国科学院上海药物研究所 | Beta-glucan GFPBW1 and its production and use |
CN103304680B (en) * | 2012-03-09 | 2017-02-08 | 中国科学院上海药物研究所 | Beta-glucan, and extraction method and application thereof |
US10688158B2 (en) | 2012-07-05 | 2020-06-23 | Nutramax Laboratories, Inc. | Compositions comprising sulforaphane or a sulforaphane precursor and a milk thistle extract or powder |
CN104906150A (en) * | 2015-06-10 | 2015-09-16 | 云南大学 | Application of grifola frondosa in preparation of anti-depression products |
CN104825499B (en) * | 2014-10-28 | 2020-04-03 | 云南大学 | Application of maitake mushroom extract in preparation of anti-depression drug |
WO2016066102A1 (en) * | 2014-10-28 | 2016-05-06 | 云南大学 | Use of maitake and maitake grifolan d component in preparation of antidepressant drug |
CN107541533B (en) * | 2017-06-13 | 2021-03-19 | 湖南民康生物技术研究所 | Preparation method of medicinal and edible fungi hypha polysaccharide polypeptide immunopotentiator |
CN108586590B (en) * | 2018-04-26 | 2020-11-10 | 中国医学科学院药用植物研究所 | Application of grifola frondosa and grifola frondosa polysaccharide peptide in promoting in vivo mercury discharge |
US20220387535A1 (en) | 2021-05-26 | 2022-12-08 | Nutramax Laboratories, Inc. | Compositions comprising sulforaphane or a sulforaphane precursor and moringa plant components |
CN114230646B (en) * | 2021-12-07 | 2023-07-04 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Antitumor grifola frondosa glycoprotein and preparation method and application thereof |
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JPH0769913A (en) * | 1993-09-02 | 1995-03-14 | Yukiguni Maitake:Kk | Production of substance having aids-curing effect |
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JPS59210901A (en) * | 1983-05-17 | 1984-11-29 | Nippon Kinoko Kenkyusho | Glucan having beta-1,6 bond-containing main chain, obtained from maitake and antineoplastic agent comprising same |
JPS60255733A (en) * | 1984-05-30 | 1985-12-17 | Nippon Beet Sugar Mfg Co Ltd | Beta-d-glucan |
JPS62209091A (en) * | 1986-03-08 | 1987-09-14 | Nippon Beet Sugar Mfg Co Ltd | Antitumor active polysaccharide |
JPH08119874A (en) * | 1994-10-24 | 1996-05-14 | Takumi Sogabe | Production of suppressor of aids virus and cancer cell |
JPH08131133A (en) * | 1994-11-09 | 1996-05-28 | Nippon Chrome Kogyo Kk | Production of health drink containing 'maitake' extract |
JPH08291078A (en) * | 1995-04-21 | 1996-11-05 | M I O:Kk | Mushroom protein for food and beverage effective in preventing and treating hypertension and hyperlipemia and having antitumor action, mushroom protein for food and beverage effective in preventing and treating obesity and having antitumor action and method for extracting these proteins |
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1996
- 1996-03-08 JP JP8051880A patent/JP2859843B2/en not_active Expired - Lifetime
-
1997
- 1997-03-06 US US08/812,795 patent/US5854404A/en not_active Expired - Lifetime
- 1997-03-07 DE DE69730420T patent/DE69730420T2/en not_active Expired - Lifetime
- 1997-03-07 CN CN97192894A patent/CN1120173C/en not_active Expired - Lifetime
- 1997-03-07 WO PCT/JP1997/000728 patent/WO1997032896A1/en active IP Right Grant
- 1997-03-07 EP EP97905467A patent/EP0893449B1/en not_active Expired - Lifetime
- 1997-03-07 AU AU22329/97A patent/AU2232997A/en not_active Abandoned
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JPH06312934A (en) * | 1993-04-30 | 1994-11-08 | Yukiguni Maitake:Kk | Production of substance having immunosuppressive effect |
JPH0769913A (en) * | 1993-09-02 | 1995-03-14 | Yukiguni Maitake:Kk | Production of substance having aids-curing effect |
JPH09238697A (en) * | 1996-03-08 | 1997-09-16 | Yukiguni Maitake:Kk | Antitumor substance extracted from grifola frondosa |
Also Published As
Publication number | Publication date |
---|---|
US5854404A (en) | 1998-12-29 |
EP0893449A1 (en) | 1999-01-27 |
EP0893449A4 (en) | 1999-04-07 |
AU2232997A (en) | 1997-09-22 |
CN1213378A (en) | 1999-04-07 |
JP2859843B2 (en) | 1999-02-24 |
DE69730420D1 (en) | 2004-09-30 |
WO1997032896A1 (en) | 1997-09-12 |
DE69730420T2 (en) | 2005-09-08 |
JPH09238697A (en) | 1997-09-16 |
EP0893449B1 (en) | 2004-08-25 |
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