CN112014450B - Method for detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode - Google Patents
Method for detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode Download PDFInfo
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Abstract
A method for electrochemically detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode adopts electrodeposition technology and electrostatic adsorption to manufacture a novel electrochemical sensor Fc-ECG/MEL/AuNPs/SPE for detecting C-reactive protein. The detection method comprises the steps of taking SPE as a substrate, taking ferrocenyl formyl glutathione (Fc [ CO-Glu-Cys-Gly-OH ], Fc-ECG) as an electrochemical probe and a recognition element, performing specific recognition on CRP, building a sensor by means of the synergistic effect of melamine and gold nano materials and double-enhancing electrochemical signals, and realizing CRP detection by using a DPV method. The method has the advantages of simple operation, time saving, low cost and lower detection limit.
Description
Technical Field
The invention belongs to the technical field of biomedical detection, and particularly relates to a method for electrochemically detecting C-reactive protein.
Background
C-reactive protein (CRP) is an important clinical biomarker for diseases such as inflammation, cardiovascular disease, coronary heart disease, septicemia and the like, and has important significance for sensitively, selectively and simply detecting CRP. The CRP detection method comprises a one-way immunodiffusion method, a latex agglutination method, an immune scattering turbidimetry method, a latex enhanced immune transmission turbidimetry method, an immune labeling technology, an enzyme-linked immunosorbent assay and the like, wherein the detection principle is that a specific anti-CRP antibody is used for reacting with CRP in a sample to be detected, and the CRP content in the sample to be detected is judged according to the diameter of a formed precipitation ring, the height of a precipitation peak, the agglutination degree or the color generation degree. Electrochemical analysis is a very effective method for researching CRP detection, and has the characteristics of real time, rapidness, sensitivity and easy miniaturization. The invention patent with the publication number of CN 109633174A discloses a C-reactive protein hypersensitivity detection method based on a biotin quantum dot probe, the time required by the whole set of detection is 110 min, and the detection is relatively time-consuming. The invention patent with the publication number of CN 109946273A discloses a fluorescent probe for detecting C-reactive protein and a preparation method thereof, wherein the detection concentration range is 0.39 mu g/mL-50 mu g/m L, and the detection range is smaller.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for electrochemically detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode, which can improve the detection specificity, sensitivity and detection range.
In order to solve the technical problem, an Fc-ECG/MEL/AuNPs/SPE-based reactive protein electrochemical biosensor is manufactured by adopting an electrodeposition technology and electrostatic adsorption. Screen Printed Electrode (SPE) as substrate, ferrocenyl formyl glutathione (Fc [ CO-Glu-Cys-Gly-OH)]Fc-ECG) is an electrochemical probe and a recognition element, specifically recognizes CRP, doubly enhances electrochemical signals by means of synergistic action of Melamine (mele) and gold nanoparticles (AuNPs) to construct a sensor, and records peak current change value of the electrochemical probe after interaction of the sensor and CRP by Differential Pulse Voltammetry (DPV) of an electrochemical workstation. Optimizing conditions such as MEL dosage, Fc-ECG dosage, Phosphate Buffer Solution (PBS) pH value, incubation time, incubation temperature and the like, drawing a working curve, and calculating to obtain the CRP concentration in the actual detection sample through the working curve. Compared with the existing method, the method has the advantages of relatively simple operation, high specificity, less time and cost consumption, and capability of reaching 0.3 ng/mL -1 The detection limit of (2).
The detection principle of the invention is as follows: and respectively modifying Fc-ECG, MEL and AuNPs on the surface of a screen printing electrode by adopting an electrodeposition technology and an electrostatic adsorption manner and utilizing a layer-by-layer self-assembly manner, constructing a sensor, and realizing the electrochemical detection of CRP. Firstly, the method comprisesSPE is the substrate of the sensor, and the electrode is preprocessed; secondly, the treated electrode is soaked in HAuCl 4 In the method, a layer of AuNPs particles is formed on the surface of a working electrode of SPE by a constant potential deposition method; dropping MEL on the surface of AuNPs/SPE, and airing at room temperature, wherein in view of the structure and chemical characteristics of MEL, the covalent bonding of groups induces aggregation of AuNPs particles in a dispersion state, so that MEL is attached to AuNPs, namely MEL/AuNPs/SPE is formed; and an Fc-ECG electroactive material is dripped on the surface of the MEL/AuNPs/SPE to serve as an electrochemical probe, and hydrogen bonding force exists between amino in the MEL and the Fc-ECG, so that the adsorption of the Fc-ECG on the electrode is facilitated, and the current response of the attached electrochemical probe is increased, thereby preparing the Fc-ECG/MEL/AuNPs/SPE electrochemical sensor. In the CRP detection process, ferrocene-labeled free sulfhydryl-containing small molecule polypeptide Fc-ECG reacts with CRP covalent bond or non-covalent bond, and the CRP content is detected through the change of electrochemical signals. And (3) detecting the electrochemical signal change value of Fc-ECG/MEL/AuNPs/SPE by using a PBS solution as a supporting electrolyte through an electrochemical workstation by using a cyclic pulse voltammetry (DPV) method, and drawing a working curve of the current and the CRP concentration so as to realize the detection of CRP.
The Fc-ECG/MEL/AuNPs/SPE electrochemical sensor is prepared according to the following steps to realize CRP detection:
step one, pretreatment. Using SPE as the substrate of the sensor and placing in H 2 SO 4 In the solution, adopting cyclic voltammetry to scan and activate for pretreatment;
and step two, preparing the AuNPs/SPE electrode. Soaking the activated bare electrode in HAuCl 4 Forming a layer of AuNPs particles on the surface of a working electrode by adopting a constant potential deposition method, and washing the electrode clean by using water after the deposition is finished to obtain an AuNPs/SPE electrode;
and step three, preparing the MEL/AuNPs/SPE electrode. Dropping MEL on the AuNPs/SPE electrode, airing at room temperature, and washing the electrode with water to obtain the MEL/AuNPs/SPE electrode;
and step four, preparing the Fc-ECG/MEL/AuNPs/SPE electrochemical sensor. Dripping Fc-ECG on an MEL/AuNPs/SPE electrode, airing at room temperature, and washing the electrode with water to obtain an Fc-ECG/MEL/AuNPs/SPE electrochemical sensor;
and step five, drawing a standard curve. And respectively dripping CRP solutions with different concentrations onto the Fc-ECG/MEL/AuNPs/SPE electrochemical sensor, airing at room temperature and then detecting. In the preparation process of the sensor, factors such as the dosage of MEL, the dosage of Fc-ECG, the pH of the reaction medium, the incubation temperature of Fc-ECG and the incubation time of CRP are considered to be optimized. After optimizing the conditions, testing and recording data by adopting a DPV technology; drawing a standard curve, and calculating the lowest detection limit of the method, wherein the detection limit can reach 0.3 ng - 1；
And step six, detecting the CRP concentration. Dripping an actual serum sample to be detected on an Fc-ECG/MEL/AuNPs/SPE sensing interface, incubating for a period of time, washing with a PBS solution, airing, putting a working electrode into the PBS solution, scanning by adopting DPV of an electrochemical workstation, and recording the peak current of the working electrode. And calculating to obtain the concentration of CRP in the actual serum sample to be detected according to the standard curve in the fifth step.
Further, in the step one, H 2 SO 4 Has a concentration of 0.5 mol.L -1 。
Further, the scanning potential range of the cyclic voltammetry in the step one is-0.4 to + 1.0V, and the scanning rate is 0.2 V.s -1 And 20 times of scanning.
Further, in the second step, the concentration of the chloroauric acid is 0.01%, the deposition potential is-0.5V, and the deposition time is 120 s.
Further, in the third step, the MEL concentration used is 10 mmol.L -1 The amount of the solution was 3. mu.L.
Further, in the fourth step, the Fc-ECG concentration used was 0.05 mmol.L -1 The amount of the solution added was 6. mu.L.
Further, in the fifth step, the optimal CRP incubation temperature is 25 ℃, and the optimal incubation time is 90 min.
Further, in the step, a supporting electrolyte solution PBS solution was used at 0.1 mol/L and pH 5.5.
Preferably, in the step, the linear scanning range is 0 to 0.6V, and the amplitude voltage is 0.05V.
Of these, both MEL and AuNPs enhance the catalytic properties of Fc-ECG, while MEL enhances better. However, when AuNPs and MEL act together, the AuNPs and the MEL have a synergistic effect, and the electrochemical signal of Fc-ECG is obviously enhanced.
Compared with the prior art, the invention has the following characteristics and advantages:
1. the Fc-ECG is an amido bond condensation compound formed by dehydrating ferrocenecarboxylic acid and reductive glutathione, on one hand, the ferrocenyl group can be used as an electrochemical probe, on the other hand, the Fc-ECG contains the reductive glutathione, and can specifically recognize CRP and improve the detection specificity.
2. AuNPs have the characteristics of high catalytic activity, large specific surface area and the like, MEL has a carbon-nitrogen conjugated heterocyclic ring structure similar to a benzene ring in the molecular structure, both the structure can enhance the electrochemical signal of Fc-ECG, and the MEL enhancing effect is better. If AuNPs and MEL act together, the synergistic effect is achieved, the capability of transferring electrons from the electrode into the solution is increased, and the electrochemical signal of the probe Fc-ECG is obviously enhanced. Compared with the traditional sensor, the novel Fc-ECG/MEL/AuNPs/SPE electrochemical biosensor prepared has a unique structure, and the attached figure 2D is an SEM image of the Fc-ECG/MEL/AuNPs/SPE electrochemical biosensor in the embodiment, so that the novel Fc-ECG/MEL/AuNPs/SPE electrochemical biosensor is smaller in size, higher in detection speed, higher in precision and higher in reliability.
Drawings
FIG. 1 is a schematic diagram of a CRP electrochemical sensor based on Fc-ECG/MEL/AuNPs/SPE dual signal amplification;
FIG. 2 SEM images of different electrodes (A: SPE, B, AuNPs/SPE, C: Fc-ECG/AuNPs/SPE, D: Fc-ECG/MEL/AuNPs/SPE);
FIG. 3 DPV curves on a sensor for different concentrations of CRP;
FIG. 4 Peak Current versus CRP concentration;
fig. 5 working curve of CRP.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
Based onThe detection principle of the method for electrochemically detecting the C-reactive protein by using the Fc-ECG/MEL/AuNPs/SPE modified electrode is shown in figure 1. Firstly, preprocessing an electrode by taking SPE as a substrate of the sensor in a layer-by-layer self-assembly mode; secondly, the treated electrode is soaked in HAuCl 4 In the method, a layer of AuNPs particles is formed on the surface of a working electrode of SPE by a constant potential deposition method; then dropping MEL on the surface of AuNPs/SPE, airing at room temperature, inducing the aggregation of dispersed AuNPs particles through covalent binding, and enabling the MEL to be attached to the AuNPs, namely forming MEL/AuNPs/SPE; and an Fc-ECG electroactive material is dripped on the surface of the MEL/AuNPs/SPE, and a hydrogen bonding force exists between amino groups in the MEL and the Fc-ECG, so that the adsorption of the Fc-ECG on an electrode is facilitated, the current response of an attached electrochemical probe is increased, and the Fc-ECG/MEL/AuNPs/SPE electrochemical sensor is prepared. In the CRP detection process, ferrocene is used for labeling small molecule polypeptide containing free sulfhydryl, the small molecule polypeptide and CRP covalent bond or non-covalent bond interact to react, and the CRP content is detected through the change of electrochemical signals.
The specific implementation steps are as follows:
step one, taking SPE as a substrate of the sensor and placing the SPE in H 2 SO 4 In the solution, the electrode is pretreated by adopting cyclic voltammetry scanning activation, and the SEM image of the SPE electrode is shown in figure 2A;
step two, soaking the activated bare electrode in 0.01% HAuCl4, forming a layer of AuNPs particles on the surface of the working electrode by adopting a constant potential deposition method, and washing the electrode clean by using water after the deposition is finished to obtain an AuNPs/SPE electrode, wherein the SEM image of the AuNPs/SPE electrode is shown in an attached figure 2B;
step three, dropping MEL on the AuNPs/SPE electrode, airing at room temperature, and washing the electrode with water to obtain the MEL/AuNPs/SPE electrode, wherein the SEM image of the MEL/AuNPs/SPE electrode is shown in the attached figure 2C;
step four, after Fc-ECG is dripped on the MEL/AuNPs/SPE electrode, the electrode is washed clean by water after being dried at room temperature, and the Fc-ECG/MEL/AuNPs/SPE electrochemical sensor is obtained, wherein the SEM image of the electrochemical sensor is shown in an attached figure 2D; as can be seen from the attached figure 2D, by the preparation method, the electrochemical sensor with unique particles and structures is gradually formed, the surface area is remarkably increased, the electrochemical probe Fc-ECG is facilitated, the capability of transferring electrons from the surface of the electrode to the solution is remarkably enhanced when AuNPs and MEL have synergistic effect, the electrochemical signal of the probe Fc-ECG is enhanced, and the high-sensitivity detection of CRP is realized;
step five, respectively dripping CRP solutions with different concentrations onto an Fc-ECG/MEL/AuNPs/SPE electrochemical sensor, testing and recording data by adopting DPV as a scanning interval under an optimized condition, wherein the concentration range of CRP is 0.001-1000 mu g/mL -1 And drawing a standard curve to obtain a linear equation, wherein the linear relationship exists between the peak current value and the peak current value:I(μA)=7.6468-0.78446lgC(μg·mL -1 ) Linear correlation coefficient R 2 To 0.99506, the minimum detection limit of this method was calculated to be 0.3 ng mL -1 ；
And step six, dripping an actual serum sample to be detected on the sensor, incubating for 90 min at room temperature, putting the working electrode into a PBS solution, scanning by adopting DPV, and recording the peak current of the working electrode. And calculating to obtain the concentration of CRP in the actual serum sample to be detected according to the standard curve in the fifth step. The results are shown in Table 1.
Table 1 results of spiking recovery of normal human serum samples (test n = 3)
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and any simple modifications, equivalent variations and modifications made to the above embodiment according to the technical essence of the present invention are within the scope of the technical solution of the present invention.
Claims (3)
1. A method for electrochemically detecting C-reactive protein based on Fc-ECG/MEL/AuNPs/SPE modified electrode comprises the following steps:
step 1, preparation of Fc-ECG/MEL/AuNPs/SPE electrochemical sensor
Using SPE as the substrate of the sensor, and feeding the electrodeLine preprocessing; is arranged at H 2 SO 4 Scanning and activating in the solution by adopting cyclic voltammetry; soaking the activated bare electrode in HAuCl 4 In the method, a layer of AuNPs particles is formed on the surface of a working electrode by adopting a constant potential deposition method, and the electrode is washed clean by water after the deposition is finished to obtain an AuNPs/SPE electrode; dripping melamine MEL on the AuNPs/SPE electrode, airing, and washing the electrode with water to obtain the MEL/AuNPs/SPE electrode; dropwise adding ferrocenyl formyl glutathione Fc-ECG on an MEL/AuNPs/SPE electrode, airing at room temperature, and washing the electrode with water to obtain an Fc-ECG/MEL/AuNPs/SPE electrochemical sensor;
step 2, respectively dripping CRP solutions with different concentrations onto an Fc-ECG/MEL/AuNPs/SPE electrochemical sensor, and airing at room temperature to be detected;
step 3, dripping an actual serum sample to be detected on an Fc-ECG/MEL/AuNPs/SPE sensing interface, incubating for a period of time, cleaning with a PBS solution to obtain a working electrode, and airing for later use;
putting the working electrode into a PBS solution, adopting DPV scanning of an electrochemical workstation, and recording the peak current of the working electrode;
and calculating to obtain the CRP concentration in the actual serum sample to be detected according to the standard curve.
2. The method of claim 1, wherein: h in said step 1 2 SO 4 Has a concentration of 0.5 mol.L -1 The scanning potential range of the cyclic voltammetry is-0.4 to + 1.0V, and the scanning rate is 0.2 V.s -1 Scanning for 20 circles; HAuCl used 4 The concentration is 0.01%, the deposition potential is-0.3V, and the deposition time is 120 s; the MEL concentration used was 10 mmol.L -1 The dropping amount is 3 mu L; the Fc-ECG concentration used was 0.05 mmol.L -1 The amount of the solution added was 6. mu.L.
3. The method of claim 1, wherein: the optimal incubation temperature of the CRP is 25 ℃, and the optimal incubation time is 90 min; the used supporting electrolyte PBS solution is 0.1 mol/L, the pH is 5.5, the linear scanning range is 0-0.6V, and the amplitude voltage is 0.05V.
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| CN110146581A (en) * | 2019-06-03 | 2019-08-20 | 桂林电子科技大学 | A method of alpha-fetoprotein is detected based on RGO-CS-Fc/Au NPs nanocomposite combination aptamers |
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| US5003065A (en) * | 1988-06-14 | 1991-03-26 | Carey Merritt | Compounds and process for measuring c-reactive protein |
| CN110146581A (en) * | 2019-06-03 | 2019-08-20 | 桂林电子科技大学 | A method of alpha-fetoprotein is detected based on RGO-CS-Fc/Au NPs nanocomposite combination aptamers |
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