CN112014434A - Water quality real-time detection method and detection device for biochemical immunoassay analyzer - Google Patents
Water quality real-time detection method and detection device for biochemical immunoassay analyzer Download PDFInfo
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Abstract
The invention discloses a real-time detection method and a real-time detection device for water quality of water for a biochemical immunoassay analyzer, which comprise a detection electrode sensor, an impedance network circuit system, a temperature sensor, a signal processing controller and an MCU (microprogrammed control Unit) controller, wherein firstly, circuit system parameters are initialized, and water is filled into a pipeline; then according to the received detection command, the digital-to-analog conversion module converts the excitation signal source sent by the digital signal synthesizer into the water to be detected, after the impedance value is tested through the impedance network circuit system, the obtained impedance value is subjected to mean value filtering and amplification processing, and after the impedance value subjected to analog-to-digital conversion is subjected to discrete Fourier transform, the impedance value is output to the MCU controller to be compared with an impedance value threshold; and calculating and analyzing the impedance value obtained by testing and the water use temperature value collected at the corresponding moment until the impedance value meets the requirement to obtain a detection impedance value, so as to obtain a conductivity value, improve the accuracy of water quality detection and reduce the hidden danger of water use.
Description
Technical Field
The invention relates to the technical field of measurement, in particular to a method and a device for detecting water quality of water for a biochemical immunoassay analyzer in real time.
Background
The analyzer is used for carrying out in vitro quantitative detection on various liquids, such as serum, urine, cerebrospinal fluid and the like, and pipelines, reaction cups, needles and the like of a liquid path of the analyzer need to be cleaned and filled with specified water before analyzing body fluid, so that the analyzer can be ensured to be normally tested in the process of carrying out project testing. When project testing is carried out, if water quality which does not meet the conductivity requirement is used, deviation of a testing result can be caused, even serious medical accidents of misdiagnosis of a patient can be caused, and currently common analytical instruments are not provided with related real-time water quality detection functions, so that great hidden danger of water consumption exists in the using process of the instruments.
Disclosure of Invention
The invention aims to provide a real-time detection method and a real-time detection device for water quality of biochemical immunoassay analyzer, which can improve the accuracy of water quality detection and reduce hidden danger of water consumption.
In order to achieve the above object, in a first aspect, the present invention provides a real-time detection device for water quality for a biochemical immunoassay analyzer, the real-time detection device for water quality for a biochemical immunoassay analyzer comprises a detection electrode sensor, an impedance network circuit system, a temperature sensor, a signal processing controller and an MCU controller, wherein the detection electrode sensor and the impedance network circuit system are both connected to the signal processing controller, and the signal processing controller and the temperature sensor are both connected to the MCU controller;
the detection electrode sensor is used for detecting an electrode pair of water quality;
the impedance network circuit system is used for carrying out impedance test on water used by the analyzer;
the temperature sensor is used for detecting the real-time temperature of water quality;
the signal processing controller is used for generating an excitation signal source required by testing and carrying out corresponding operation processing on received and generated data;
and the MCU controller is used for receiving the data uploaded by the signal processing controller and the temperature sensor and outputting a corresponding conductivity value.
The signal processing controller comprises a digital signal synthesizer, a digital-to-analog conversion module, an analog-to-digital conversion module, a filtering and amplifying module and a discrete Fourier operation module, wherein the digital signal synthesizer is connected with the digital-to-analog conversion module, the digital-to-analog conversion module is connected with the detection electrode sensor, the filtering and amplifying module is connected with the impedance network circuit system, the analog-to-digital conversion module is connected with the filtering and amplifying module, and the discrete Fourier operation module is connected with the analog-to-digital conversion module and the MCU controller;
the digital signal synthesizer is used for generating an excitation signal source required by the water quality testing condition;
the digital-to-analog conversion module is used for converting the digital quantity of the digital signal synthesizer into a voltage value, adjusting the voltage value and outputting the voltage value to the detection electrode sensor;
the filtering and amplifying module is used for filtering and amplifying the feedback signal;
the analog-to-digital conversion module is used for acquiring the analog signal of the impedance network circuit system and converting the analog signal into digital quantity;
and the discrete Fourier operation module is used for performing discrete Fourier operation on the output value of the analog-to-digital conversion module.
In a second aspect, the invention provides a real-time detection method for water quality of a biochemical immunoassay analyzer, comprising the following steps:
initializing circuit system parameters, filling water into the pipeline, and waiting for detection;
starting an impedance test according to the obtained detection command, and carrying out mean value filtering on the obtained impedance value;
and collecting temperature values corresponding to the test moments, and calculating the conductivity value by combining the filtered impedance values.
Wherein, initializing circuit system parameters, and after pouring water into the pipeline, waiting for the detection, include:
initializing circuit system parameters, setting signal frequency, voltage signal amplitude value and impedance value threshold, and waiting for the sending of a detection command after filling the pipeline with the detected water.
Wherein, start the impedance test according to the detection command who obtains to carry out mean value filtering to the impedance value who obtains, include:
according to the received detection command, the digital-to-analog conversion module converts an excitation signal source sent by the digital signal synthesizer into the detected water and outputs the converted excitation signal source to the detected water, after an impedance value is tested through the impedance network circuit system, the obtained impedance value is subjected to mean value filtering and amplification processing, and after discrete Fourier transform is performed on the impedance value subjected to analog-to-digital conversion, the impedance value is output to the MCU controller and compared with the impedance value threshold.
Wherein, start the impedance test according to the detection command who obtains to carry out mean value filtering to the impedance value who obtains, still include:
if the impedance value received by the MCU controller is larger than the impedance value threshold, the signal frequency and the voltage signal amplitude value of the excitation signal source are readjusted until the impedance value obtained by retesting is smaller than or equal to the impedance value threshold, and a detected impedance value is obtained.
The method for acquiring the temperature value corresponding to the test moment and calculating the conductivity value by combining the filtered impedance value comprises the following steps:
and acquiring a water use temperature value corresponding to the moment of detecting the impedance value by using a temperature sensor, and calculating and analyzing by combining the detected impedance value to obtain a conductivity value after temperature compensation.
The invention relates to a real-time detection method and a real-time detection device for water quality of water for a biochemical immunoassay analyzer, wherein the real-time detection device for water quality of water for the biochemical immunoassay analyzer comprises a detection electrode sensor, an impedance network circuit system, a temperature sensor, a signal processing controller and an MCU (microprogrammed control Unit) controller, wherein the signal processing controller comprises a digital signal synthesizer, a digital-to-analog conversion module, an analog-to-digital conversion module, a filtering and amplifying module and a discrete Fourier operation module; then according to the received detection command, the digital-to-analog conversion module converts the excitation signal source sent by the digital signal synthesizer into an output signal to be detected, after the impedance value is tested through the impedance network circuit system, the obtained impedance value is subjected to mean filtering and amplification processing, and after the impedance value subjected to analog-to-digital conversion is subjected to discrete Fourier transform, the output signal is output to the MCU controller to be compared with an impedance value threshold; and calculating and analyzing the detected impedance value and the water use temperature value acquired at the corresponding moment until the impedance value obtained by the test is less than or equal to the impedance value threshold value to obtain a conductivity value, so that the accuracy of water quality detection is improved, and the hidden danger of water use is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a real-time water quality detection device for a biochemical immunoassay analyzer according to the present invention.
FIG. 2 is a schematic step diagram of a real-time detection method for water quality of a biochemical immunoassay analyzer according to the present invention.
Fig. 3 is a diagram of the structure and application of the analyzer of the present invention.
FIG. 4 is a schematic flow chart of real-time detection of water quality for a biochemical immunoassay analyzer provided by the present invention.
The device comprises a detection electrode sensor, a 2-impedance network circuit system, a 3-temperature sensor, a 4-signal processing controller, a 5-MCU controller, a 41-digital signal synthesizer, a 42-digital-to-analog conversion module, a 43-analog-to-digital conversion module, a 44-filtering and amplifying module and a 45-discrete Fourier operation module.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1, the present invention provides a real-time detection apparatus for water quality for a biochemical immunoassay analyzer, the real-time detection apparatus for water quality for a biochemical immunoassay analyzer includes a detection electrode sensor 1, an impedance network circuit system 2, a temperature sensor 3, a signal processing controller 4 and an MCU controller 5, the detection electrode sensor 1 and the impedance network circuit system 2 are both connected to the signal processing controller 4, and the signal processing controller 4 and the temperature sensor 3 are both connected to the MCU controller 5;
the detection electrode sensor 1 is used for detecting an electrode pair of water quality;
the impedance network circuit system 2 is used for carrying out impedance test on water used by the analyzer;
the temperature sensor 3 is used for detecting the real-time temperature of water quality;
the signal processing controller 4 is used for generating an excitation signal source required by testing and performing corresponding operation processing on received and generated data;
and the MCU controller 5 is used for receiving the data uploaded by the signal processing controller 4 and the temperature sensor 3 and outputting a corresponding conductivity value.
In this embodiment, as shown in the structure and application diagram of the analyzer provided in fig. 3, the analyzer includes a liquid path interface board, a main water inlet valve, a drain valve, a buffer tank, a water quality detection device, and other liquid path pipelines, and this schematic diagram is only a partial principle of the whole liquid path system. The analyzer induces the water quality condition from the liquid path pipeline through the electrodes of the water quality detection module. The water inlet is an input interface of an external pipeline, and the power device in the liquid path is started to fill the buffer tank with water by opening the main water inlet valve. When the instrumented machine delivers goods, the water draining process is completed through the drain valve. After the liquid way is filled, the water quality used by the whole machine is monitored by the water quality device added in the liquid way pipeline, and the quality of water used by the instrument is ensured.
When the water quality detection device is used for detection, firstly, an excitation signal is sent to the detected water through the signal processing controller 4, the electrode pair in the detected water quality is detected through the detection electrode sensor 1, the detection electrode sensor 1 is an electrode made of metal, the electrode has a fixed conductance constant, and the corresponding conductivity can be converted only by testing a measured impedance value obtained through the electrode; and then the impedance value is transmitted to the impedance network circuit system 2 for impedance test, after a corresponding impedance value is obtained, the impedance value is subjected to corresponding operation and conversion operation through the signal processing controller 4 again, and then the impedance value is output to the MCU controller 5, and is compared and adjusted with an impedance value threshold value until a detection impedance value smaller than or equal to the impedance value threshold value is obtained, and in the actual detection process, the default excitation signal frequency is preferentially adopted for impedance test on the water quality. If the detection range exceeds the range of the test value, the software can adjust the resistance value in the feedback network at the moment, and the test is carried out again at the same frequency. After the feedback resistance is adjusted, the impedance is tested again until a range of impedance values is determined. The design patent considers the practical application in the water quality detection of the analyzer, so the measurable conductivity range of the analyzer is determined at the beginning of design; meanwhile, the temperature sensor 3 is used for collecting the temperature value of the detected water quality at the corresponding moment, data operation and analysis are carried out on the temperature value and the detected impedance value, the conductivity value after temperature compensation is obtained according to the conversion formula of the conductivity at different temperatures, the conductivity value is compared with a threshold value, and the MCU controller 5 automatically enters dormancy after the result output is completed once every time. And restarting conversion and result output until a new starting instruction arrives, and combining and converting the impedance value and the temperature value, so that the influence of the temperature on the conductivity can be reduced, whether the water for the analyzer meets the requirement can be effectively detected, and the reliability of the detection result of the analyzer is ensured. Because the whole detection module adopts the signal processing controller 4 to complete the design of the module, the problem that signals generated by the lap joint of each circuit module are unstable and are easy to interfere is avoided. The circuit design and the realization are simplified, thereby increasing the monitoring of the liquid temperature and improving the reliability and the accuracy of the design; the detection device is added in an internal liquid path system of the instrument, so that the water consumption condition of the instrument can be monitored in real time, the quality of water for instrument testing is guaranteed, the correctness of the detection result of the analyzer is ensured, and the hidden danger of water consumption is reduced.
Further, the signal processing controller 4 includes a digital signal synthesizer 41, a digital-to-analog conversion module 42, an analog-to-digital conversion module 43, a filtering and amplifying module 44, and a discrete fourier operation module 45, where the digital signal synthesizer 41 is connected to the digital-to-analog conversion module 42, the digital-to-analog conversion module 42 is connected to the detection electrode sensor 1, the filtering and amplifying module 44 is connected to the impedance network circuit system 2, the analog-to-digital conversion module 43 is connected to the filtering and amplifying module 44, and the discrete fourier operation module 45 is connected to the analog-to-digital conversion module 43 and the MCU controller 5;
the digital signal synthesizer 41 is used for generating an excitation signal source required by the water quality testing condition;
the digital-to-analog conversion module 42 is configured to convert the digital quantity of the digital signal synthesizer 41 into a voltage value, adjust the voltage value, and output the voltage value to the detection electrode sensor 1;
the filtering and amplifying module 44 is configured to filter and amplify the feedback signal;
the analog-to-digital conversion module 43 is configured to acquire an analog signal of the impedance network circuit system 2 and convert the analog signal into a digital quantity;
the discrete fourier operation module 45 is configured to perform discrete fourier operation on the output value of the analog-to-digital conversion module 43.
In this embodiment, after the detection device receives the detection command, the digital signal synthesizer 41 is controlled to send out the excitation signal source required for testing the water quality condition, wherein both the frequency and the amplitude of the excitation signal can be flexibly adjusted along with the testing process, so as to meet the requirement of the adaptive test. The digital-to-analog conversion module 42 converts the digital quantity of the excitation signal source into a voltage value and adjusts the voltage value, and then outputs the voltage value to the detection electrode sensor 1, when the impedance network circuit system 2 performs an impedance test to obtain a corresponding impedance value, the filtering and amplifying module 44 is used for filtering the impedance value, removing noise to realize signal conversion and acquisition functions, and in order to perform a stable conductivity test result, data in the acquisition process is filtered in real time to ensure the stability and reliability of the result; then, the analog-to-digital conversion module 43 is used for analog-to-digital conversion, and the discrete fourier operation module 45 is used for operating the digital value after analog-to-digital conversion, so that the signal-to-noise ratio is improved and the corresponding result value is output. Due to the adoption of the integrated signal processing controller 4, the signal interference can be effectively reduced, and the anti-interference capability is improved. While considering that the results of the conductivity are greatly affected by the temperature. Therefore, the device has the function of collecting temperature signals, and the reliability of the test result is further improved.
Referring to fig. 2 and 4, the present invention provides a real-time detection method for water quality of a biochemical immunoassay analyzer, comprising:
s101, initializing circuit system parameters, filling water into the pipeline, and waiting for detection.
Specifically, a circuit system in the water quality detection device is initialized, a signal frequency and a voltage signal amplitude value used by an excitation signal are selected or set, feedback impedance value information of an initial impedance network is configured, then the detected water quality is poured into a pipeline in an analyzer, the water quality detection device is guaranteed to be completely immersed in water, and then a detection command is waited to be sent.
And S102, starting an impedance test according to the obtained detection command, and carrying out mean value filtering on the obtained impedance value.
Specifically, after receiving a detection command, the digital signal synthesizer 41 is controlled to send out an excitation signal source required for testing the water quality condition, the digital quantity of the excitation signal source is converted into a voltage value by the digital-to-analog conversion module 42 and is adjusted, and then the voltage value is output to the detection electrode sensor 1, after the impedance network circuit system 2 performs an impedance test to obtain a corresponding impedance value, the filtering and amplification module 44 is used for filtering the impedance value, removing noise to realize signal conversion and acquisition functions, and in order to perform a stable conductivity test result, data in the acquisition process is filtered in real time to ensure the stability and reliability of the result; then, the analog-to-digital conversion module 43 is used for analog-to-digital conversion, the discrete fourier operation module 45 is used for operating the digital value after the analog-to-digital conversion, the digital value is output to the MCU controller 5 to be compared with an impedance value threshold, if the impedance value received by the MCU controller 5 is greater than the impedance value threshold, the signal frequency and the voltage signal amplitude value of the excitation signal source are readjusted, and after the adjustment is completed, the test is performed again until the impedance value obtained by the retest is less than or equal to the impedance value threshold, so as to obtain a final detected impedance value, which is used as the test use value of this time. When the impedance value is tested, multiple sampling and averaging filtering are performed, and the signal-to-noise ratio is improved.
S103, collecting temperature values corresponding to the test moments, and calculating a conductivity value by combining the filtered impedance values.
Specifically, the current water temperature value at the moment corresponding to the detected impedance value is read and obtained. The signal processing amplification and filtering processing are performed with respect to the temperature using the analog temperature sensor 3 in the present design example. The acquisition of the temperature signal is not limited to the analog method, and a sensor which is output in a digital mode can also be adopted, and the MCU controller 5 carries out data operation and analysis according to the acquired impedance value and the current water temperature value to obtain the current conductivity value. And obtaining the conductivity value after temperature compensation according to a conversion formula of the conductivity at different temperatures, so that the measured result is reliable.
The method further comprises the following steps:
and S104, comparing the conductivity value with an instrument threshold value, and carrying out corresponding early warning operation.
Specifically, through the interaction between the MCU controller 5 and the host command, the MCU controller 5 outputs the current conductivity value and the current water temperature value through the communication interface; and after outputting the conductivity value to the host, the MCU controller 5 waits for the next test instruction. The MCU controller 5 automatically enters the sleep mode after finishing outputting the result once every time. Restarting conversion and result output until a new starting instruction arrives; after receiving the output conductivity value, the host determines whether the output conductivity value is within a required range. If the water quality is qualified, the alarm is given, the water quality is unqualified, and attention is paid to avoid influencing the test result.
The invention relates to a real-time detection method and a real-time detection device for water quality used for a biochemical immunoassay analyzer, wherein the real-time detection device for water quality used for the biochemical immunoassay analyzer comprises a detection electrode sensor 1, an impedance network circuit system 2, a temperature sensor 3, a signal processing controller 4 and an MCU (microprogrammed control Unit) controller 5, wherein the signal processing controller 4 comprises a digital signal synthesizer 41, a digital-to-analog conversion module 42, an analog-to-digital conversion module 43, a filtering and amplifying module 44 and a discrete Fourier operation module 45, firstly, circuit system parameters are initialized, and after water is filled into a pipeline, a detection command is waited to be issued; then, according to the received detection command, the digital-to-analog conversion module 42 converts the excitation signal source sent by the digital signal synthesizer 41 into an output to be detected, performs impedance value test through the impedance network circuit system 2, performs mean filtering and amplification processing on the obtained impedance value, performs discrete fourier transform on the impedance value after analog-to-digital conversion, and outputs the impedance value to the MCU controller 5 to compare the impedance value with an impedance value threshold; and calculating and analyzing the detected impedance value and the water use temperature value acquired at the corresponding moment until the impedance value obtained by the test is less than or equal to the impedance value threshold value to obtain a conductivity value, so that the accuracy of water quality detection is improved, and the hidden danger of water use is reduced.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A water quality real-time detection device for a biochemical immunoassay analyzer, which is characterized in that,
the water quality real-time detection device for the biochemical immunoassay analyzer comprises a detection electrode sensor, an impedance network circuit system, a temperature sensor, a signal processing controller and an MCU (microprogrammed control Unit) controller, wherein the detection electrode sensor and the impedance network circuit system are both connected with the signal processing controller, and the signal processing controller and the temperature sensor are both connected with the MCU controller;
the detection electrode sensor is used for detecting an electrode pair of water quality;
the impedance network circuit system is used for carrying out impedance test on water used by the analyzer;
the temperature sensor is used for detecting the real-time temperature of water quality;
the signal processing controller is used for generating an excitation signal source required by testing and carrying out corresponding operation processing on received and generated data;
and the MCU controller is used for receiving the data uploaded by the signal processing controller and the temperature sensor and outputting a corresponding conductivity value.
2. The device for real-time detection of water quality for biochemical immunoassay analyzer according to claim 1,
the signal processing controller comprises a digital signal synthesizer, a digital-to-analog conversion module, an analog-to-digital conversion module, a filtering and amplifying module and a discrete Fourier operation module, wherein the digital signal synthesizer is connected with the digital-to-analog conversion module, the digital-to-analog conversion module is connected with the detection electrode sensor, the filtering and amplifying module is connected with the impedance network circuit system, the analog-to-digital conversion module is connected with the filtering and amplifying module, and the discrete Fourier operation module is connected with the analog-to-digital conversion module and the MCU controller;
the digital signal synthesizer is used for generating an excitation signal source required by the water quality testing condition;
the digital-to-analog conversion module is used for converting the digital quantity of the digital signal synthesizer into a voltage value, adjusting the voltage value and outputting the voltage value to the detection electrode sensor;
the filtering and amplifying module is used for filtering and amplifying the feedback signal;
the analog-to-digital conversion module is used for acquiring the analog signal of the impedance network circuit system and converting the analog signal into digital quantity;
and the discrete Fourier operation module is used for performing discrete Fourier operation on the output value of the analog-to-digital conversion module.
3. A real-time detection method for water quality of biochemical immunoassay analyzer is characterized by comprising the following steps:
initializing circuit system parameters, filling water into the pipeline, and waiting for detection;
starting an impedance test according to the obtained detection command, and carrying out mean value filtering on the obtained impedance value;
and collecting temperature values corresponding to the test moments, and calculating the conductivity value by combining the filtered impedance values.
4. The method for real-time detection of water quality for biochemical immunoassay analyzer according to claim 3, wherein initializing circuit system parameters and waiting for detection after filling water into the pipeline comprises:
initializing circuit system parameters, setting signal frequency, voltage signal amplitude value and impedance value threshold, and waiting for the sending of a detection command after filling the pipeline with the detected water.
5. The method for real-time detection of water quality for biochemical immunoassay analyzer according to claim 4, wherein the starting of the impedance test according to the obtained detection command and the mean filtering of the obtained impedance values comprises:
according to the received detection command, the digital-to-analog conversion module converts an excitation signal source sent by the digital signal synthesizer into the detected water and outputs the converted excitation signal source to the detected water, after an impedance value is tested through the impedance network circuit system, the obtained impedance value is subjected to mean value filtering and amplification processing, and after discrete Fourier transform is performed on the impedance value subjected to analog-to-digital conversion, the impedance value is output to the MCU controller and compared with the impedance value threshold.
6. The method of claim 5, wherein the impedance test is started according to the obtained detection command, and the obtained impedance value is subjected to mean filtering, and further comprising:
if the impedance value received by the MCU controller is larger than the impedance value threshold, the signal frequency and the voltage signal amplitude value of the excitation signal source are readjusted until the impedance value obtained by retesting is smaller than or equal to the impedance value threshold, and a detected impedance value is obtained.
7. The method of claim 6, wherein the step of collecting a temperature value corresponding to a test time and calculating a conductivity value by combining the filtered impedance value comprises:
and acquiring a water use temperature value corresponding to the moment of detecting the impedance value by using a temperature sensor, and calculating and analyzing by combining the detected impedance value to obtain a conductivity value after temperature compensation.
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