CN112014183B - Preparation device and marking method for experimental animal embryo bone specimen - Google Patents
Preparation device and marking method for experimental animal embryo bone specimen Download PDFInfo
- Publication number
- CN112014183B CN112014183B CN202010907715.0A CN202010907715A CN112014183B CN 112014183 B CN112014183 B CN 112014183B CN 202010907715 A CN202010907715 A CN 202010907715A CN 112014183 B CN112014183 B CN 112014183B
- Authority
- CN
- China
- Prior art keywords
- pull rope
- child
- solution
- winding roller
- winding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000010171 animal model Methods 0.000 title claims abstract description 16
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 65
- 238000004804 winding Methods 0.000 claims abstract description 64
- 238000003825 pressing Methods 0.000 claims abstract description 35
- 238000004043 dyeing Methods 0.000 claims abstract description 25
- 229920000742 Cotton Polymers 0.000 claims description 12
- 210000001835 viscera Anatomy 0.000 claims description 7
- 210000000845 cartilage Anatomy 0.000 claims description 6
- 238000010186 staining Methods 0.000 claims description 6
- 239000001045 blue dye Substances 0.000 claims description 5
- 210000000683 abdominal cavity Anatomy 0.000 claims description 4
- 239000003086 colorant Substances 0.000 claims description 4
- 230000011164 ossification Effects 0.000 claims description 4
- 206010015548 Euthanasia Diseases 0.000 claims description 3
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 claims description 3
- 239000000975 dye Substances 0.000 claims description 3
- 239000001044 red dye Substances 0.000 claims description 3
- 238000010030 laminating Methods 0.000 claims description 2
- 230000001605 fetal effect Effects 0.000 claims 3
- 210000002082 fibula Anatomy 0.000 claims 1
- 238000002372 labelling Methods 0.000 claims 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 230000008595 infiltration Effects 0.000 description 5
- 238000001764 infiltration Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000985 reactive dye Substances 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 3
- 210000003754 fetus Anatomy 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000700159 Rattus Species 0.000 description 2
- 238000010009 beating Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 231100000378 teratogenic Toxicity 0.000 description 2
- 230000003390 teratogenic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Abstract
The invention discloses a preparation device and a marking method of experimental animal embryo bone specimens, comprising a plurality of solution boxes, a winding component and dyeing components arranged in the solution boxes, wherein the solution boxes are distributed at intervals, at least one dyeing component is arranged in any solution box, and different dye solutions are arranged in the solution boxes; the dyeing assembly comprises a plurality of clamping blocks which are arranged in parallel, and a sample groove is arranged between any two clamping blocks; grooves are formed in two ends of the solution tank, supporting mechanisms are arranged on the inner sides of the grooves, and pressing mechanisms are connected to the inner walls of the solution tank in a matched mode; the winding component comprises a first winding roller and a second winding roller, at least one first pull rope is wound on the first winding roller, at least one second pull rope is wound on the second winding roller, and the first pull rope corresponds to the position direction of the sample groove; a plurality of salient points are arranged on the first pull rope and the second pull rope at intervals, and the first pull rope and the second pull rope can be wound through the first winding roller and the second winding roller respectively.
Description
Technical Field
The invention relates to bone marking, in particular to a preparation device and a marking method of experimental animal embryo bone specimens.
Background
The current teratogenic test is a conventional healthy toxicological test which is required for drug registration, pesticide registration and chemical registration. Teratogenic tests are administered to rats or rabbits during pregnancy, and during caesarean section at the end of pregnancy, all the fetuses are taken for appearance, viscera and skeleton examination, wherein the preparation of skeleton specimens is particularly complex.
Average 15 litters in one litter of the rat (at least half of the litters in each litter are used for preparing bone specimens), and average 7 litters in one litter of the rabbit (all bone specimens need to be prepared). The young fetus needs to be corroded, dyed, transparent and the like, and can be manufactured into a young fetus specimen for examination after about one week.
Because each child needs to be inspected, each child bone specimen must be labeled. At present, common methods in the industry are to put the litters in the same nest together in a concentrated way, make box labels (information such as project numbers, good parent mice, number of litters and the like are marked), mark each litter in the box is often a superplastic small label (information such as the number of litters is marked on the label), and then want to plug the small label into the abdominal cavity of the litter and want to tie the small label on ribs. The liquid needs to be continuously changed in the process of manufacturing the specimen, the small labels of the individual child are likely to fall off in the process, the time and the labor are consumed for manufacturing the small labels, and the cost is high. Therefore, the patent designer considers a simpler method, and the time, the labor and the money are saved.
Disclosure of Invention
The invention aims to provide a preparation device and a marking method for experimental animal embryo bone specimens, which overcome the defects in the prior art.
In order to solve the technical problems, the technical scheme of the invention is as follows: the preparation device of experimental animal embryo bone specimen comprises a plurality of solution boxes, a winding component and a dyeing component arranged in the solution boxes,
the plurality of solution boxes are distributed at intervals, at least one dyeing component is arranged in any one of the solution boxes, and different dye solutions are arranged in the plurality of solution boxes;
the dyeing assembly comprises a plurality of clamping blocks which are arranged in parallel, and a sample groove is arranged between any two clamping blocks;
grooves are formed in two ends of the solution tank, supporting mechanisms are arranged on the inner sides of the grooves, and pressing mechanisms are connected to the inner wall of the solution tank in a matched mode;
the winding assembly comprises a first winding roller and a second winding roller, at least one first pull rope is wound on the first winding roller, at least one second pull rope is wound on the second winding roller, and the first pull rope corresponds to the sample groove in position;
the first pull rope and the second pull rope are respectively provided with a plurality of protruding points at intervals, and the first pull rope and the second pull rope can be wound by the first winding roller and the second winding roller respectively.
Preferably, the pressing mechanisms are in one-to-one correspondence with the sample grooves, and the pressing mechanisms are provided with anti-deflection grooves, and the anti-deflection grooves are in position correspondence with the sample grooves.
Preferably, the first pull rope is located below the pressing mechanism, and the first pull rope is arranged on the inner side of the anti-deflection groove.
Preferably, the support mechanism is provided with at least one anti-deflection groove, the second pull rope is located above the support mechanism, and the second pull rope is located at the inner side of the anti-deflection groove.
Preferably, a sliding groove is formed in the solution tank, the sliding groove is connected with the pressing mechanism in a matched mode through a sliding mechanism, and the sliding mechanism can slide along the sliding groove.
Preferably, a rope groove is arranged between any two clamping blocks, a plurality of adjusting mechanisms are arranged at the bottoms of the clamping blocks, and the adjusting mechanisms can stretch out and draw back.
Preferably, the number of the first winding rollers is two, the two first winding rollers rotate in the same direction at the same speed, the number of the second winding rollers is two, the two second winding rollers rotate in the same direction at the same speed, and the rotating speeds of the first winding rollers and the second winding rollers can be the same or different.
The invention also provides a marking method of the experimental animal embryo bone specimen, which comprises the following steps:
s1, removing viscera and fat after euthanasia of a child, opening an abdominal cavity, removing the viscera of a pleuroperitoneal cavity by using ophthalmic forceps, peeling skin by using forceps, and removing fat at the back of the neck and the shoulder blade by using scissors and the ophthalmic forceps;
s2, tying cotton threads with different colors on the waist of the child to identify child with different numbers;
s3, cartilage staining: clamping the child between a first pull rope and a second pull rope by adjusting the rotating speeds of the first winding roller and the second winding roller, transferring the child into a first solution tank, and pressing the child into a sample tank by a pressing mechanism for dyeing for 1-2 days;
s4, osteogenesis staining: after the cartilage is dyed, the height of the pressing mechanism is adjusted through the adjusting mechanism, the young child is clamped and hung through the first pull rope and the second pull rope, meanwhile, the first winding roller and the second winding roller move in a matched mode at the same speed, the young child is transferred to the second solution tank, and the young child is pressed into the sample tank through the pressing mechanism to be dyed for 1-3 days.
Preferably, in the dyeing process, the first pull rope slides along the sample groove, and the salient points on the first pull rope are contacted with the surface of the child so as to flap the bone surface of the child.
Preferably, 100mg/L of Alxin blue dye liquor is placed in the first solution box, and 100mg/L of alizarin red dye liquor is placed in the second solution box.
The invention solves the defects existing in the background technology, and has the following beneficial effects:
(1) The first stay cord and the second stay cord clamp the child after the mark in the middle, then move to the same direction through first roller and second roller with the same speed, when transporting child to first solution case top, press the child to the sample inslot through pressing mechanism, at this moment, stop the second stay cord and remove, drive first stay cord laminating child top layer through first roller and remove, the in-process that first stay cord removed can beat child surface through the bump, the bump can also stir the solution near child in addition, make child can more complete infiltration allrin blue dye liquor, the infiltration effect is better.
(2) After the first solution box is soaked, the supporting mechanism is matched with the pressing mechanism, the child is clamped between the first pull rope and the second pull rope and conveyed into the second solution box for secondary soaking, the supporting mechanism is matched with the pressing mechanism, automatic transfer of the child can be achieved, automation of making of bone specimens of the child is achieved, and operation is simple and flexible.
(3) The cotton thread is a colored cotton thread dyed by reactive dyes commonly used in the market, is convenient to purchase, is easy to obtain, is low in price, saves money, is tied on the middle part of the waist and abdomen when a specimen is manufactured, and is simpler to operate compared with the conventional method of tying a small label on the rib on the abdomen. Time and labor saving
(4) The adopted colored cotton thread is light and handy, and is not easy to fall off or break. Is suitable for liquid exchange and shaking in specimen preparation. The effect is good, and the common reactive dye of the colored cotton thread is not affected in the processes of corrosion, dyeing and transparency and is not decolorized; can also be stored in glycerol for a long period without decolorization.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings may be obtained according to the drawings without inventive effort to those skilled in the art.
FIG. 1 is a schematic view of a partial perspective structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the first solution tank of the present invention;
FIG. 3 is a top view of the first solution tank of the present invention;
FIG. 4 is a partial cross-sectional view of a first solution tank of the present invention;
FIG. 5 is a schematic view of the dyeing assembly structure of the present invention;
FIG. 6 is a table of litter size identification according to the invention;
specifically, 1, a first winding roller, 2, a second pull rope, 3, a second winding roller, 4, a first pull rope, 5, a sliding mechanism, 6, a convex point, 7, a supporting mechanism, 8, a first solution box, 9, a pressing mechanism, 10, a clamping block, 11, a second solution box, 12, an anti-deviation groove, 13, an adjusting mechanism, 14, a specimen groove, 15, a sliding groove, 16 and a rope groove.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the scope of the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may include one or more of the feature, either explicitly or implicitly. In the description of the invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art in a specific case.
As shown in fig. 1-5, the invention discloses a preparation device of experimental animal embryo bone specimens, which comprises a plurality of solution boxes, a winding component and a dyeing component arranged in the solution boxes,
the plurality of solution boxes are distributed at intervals, at least one dyeing component is arranged in any one solution box, and different dyeing solutions are arranged in the plurality of solution boxes;
the dyeing assembly comprises a plurality of clamping blocks 10 which are arranged in parallel, and a sample groove 14 is arranged between any two clamping blocks 10;
grooves are formed in the two ends of the solution tank, supporting mechanisms 7 are arranged on the inner sides of the grooves, and pressing mechanisms 9 are connected to the inner walls of the solution tank in a matched mode;
the winding assembly comprises a first winding roller and a second winding roller, at least one first pull rope 4 is wound on the first winding roller, at least one second pull rope 2 is wound on the second winding roller, and the first pull rope 4 corresponds to the sample groove 14 in position;
a plurality of salient points 6 are arranged on the first pull rope 4 and the second pull rope 2 at intervals, and the first pull rope 4 and the second pull rope 2 can be wound by a first winding roller and a second winding roller respectively.
The first pull rope 4 and the second pull rope 2 clamp the marked child in the middle, then the first roller 1 and the second roller 3 move in the same direction, when the child is conveyed to the upper side of the first solution tank 8, the child is pressed into the sample groove 14 through the pressing mechanism 9, at the moment, the second pull rope 2 is stopped to move, the first pull rope 4 is driven to be attached to the surface layer of the child only through the first roller 1, the surface of the child can be beaten through the convex points 6 in the moving process of the first pull rope 4, in addition, the convex points 6 can stir the solution near the child, so that the child can be more completely infiltrated with the Alxin blue dye solution, and the infiltration effect is better.
After the first solution box 8 is soaked, the supporting mechanism 7 is matched with the pressing mechanism 9, the child is clamped between the first pull rope 4 and the second pull rope 2 and conveyed into the second solution box 11 for secondary soaking, the supporting mechanism 7 is matched with the pressing mechanism 9, automatic transfer of the child can be achieved, automation of making of bone specimens of the child is achieved, and the operation is simple and flexible.
According to the embodiment of the invention, the pressing mechanisms 9 are in one-to-one correspondence with the sample grooves 14, the pressing mechanisms 9 are provided with the anti-deflection grooves 12, and the anti-deflection grooves 12 correspond to the sample grooves 14 in position.
According to the embodiment of the invention, the first pull rope 4 is positioned below the pressing mechanism 9, and the first pull rope 4 is positioned inside the deviation preventing groove 12.
According to the embodiment of the invention, the supporting mechanism 7 is provided with at least one deviation preventing groove 12, the second pull rope 2 is positioned above the supporting mechanism 7, and the second pull rope 2 is positioned at the inner side of the deviation preventing groove 12.
According to the embodiment of the invention, the inside of the solution tank is provided with the sliding groove 15, the sliding groove 15 is connected with the pressing mechanism 9 in a matched manner through the sliding mechanism 5, and the sliding mechanism 5 can slide along the sliding groove 15.
According to the embodiment of the invention, a rope groove 16 is arranged between any two clamping blocks 10, a plurality of adjusting mechanisms 13 are arranged at the bottoms of the clamping blocks 10, and the adjusting mechanisms 13 can stretch and retract.
It should be noted that the plurality of clamping blocks are distributed in parallel, each clamping block can be independently adjusted and stretched through the adjusting mechanism below, two clamping blocks located at the outermost side of the dyeing assembly are of symmetrical L-shaped structures, and the rest clamping blocks are of T-shaped structures.
According to the embodiment of the invention, the number of the first winding rollers is two, the two first winding rollers rotate in the same direction at the same speed, the number of the second winding rollers are two, the two second winding rollers rotate in the same direction at the same speed, and the rotating speeds of the first winding rollers and the second winding rollers can be the same or different.
It should be noted that, when the rotational speed of the first wind-up roll is the same with the rotational speed of the second wind-up roll, be used for transporting the sample, when the rotational speed of the first wind-up roll is greater than the second wind-up roll, bump 6 on the first stay cord 4 is used for beating the sample surface, makes sample upper surface infiltration completely, otherwise, when the rotational speed of the first wind-up roll is less than the second wind-up roll, bump 6 on the second stay cord 2 is used for beating the sample lower surface, makes the lower surface infiltration completely, and in carrying out sample dyeing in-process adjustment mechanism 13 can stretch out and draw back the height that adjustment sample groove 14 is located the solution incasement, makes the sample can get into in the solution completely, and sample preparation is simple nimble.
As shown in fig. 6, the present invention discloses a litter size identification table.
The invention also provides a marking method of the experimental animal embryo bone specimen, which comprises the following steps:
s1, removing viscera and fat after euthanasia of a child, opening an abdominal cavity, removing the viscera of a pleuroperitoneal cavity by using ophthalmic forceps, peeling skin by using forceps, and removing fat at the back of the neck and the shoulder blade by using scissors and the ophthalmic forceps;
s2, tying cotton threads with different colors on the waist of the child to identify child with different numbers;
s3, cartilage staining: clamping the child between the first pull rope 4 and the second pull rope 2 by adjusting the rotation speeds of the first winding roller and the second winding roller, transferring the child into the first solution tank 8, and pressing the child into the sample tank 14 by the pressing mechanism 9 for dyeing for 1-2 days;
s4, osteogenesis staining: after the cartilage is dyed, the height of the pressing mechanism 9 is adjusted through the adjusting mechanism 13, the young child is clamped and hung through the first pull rope 4 and the second pull rope 2, meanwhile, the first winding roller and the second winding roller move in a matched mode at the same speed, the young child is transferred to the second solution tank 11, and the young child is pressed into the sample tank 14 through the pressing mechanism 9 to be dyed for 1 to 3 days.
According to the embodiment of the invention, in the dyeing process, the first pull rope 4 slides along the sample groove 14, and the salient points 6 on the first pull rope 4 are contacted with the surface of the child so as to flap the bone surface of the child.
The baby can be a baby of a big mouse or a baby of a rabbit, after cotton threads with different colors are tied on the waist of the baby, the baby is rinsed in water, and then the same baby nest is placed in the same container and is stuck with a nest tag for distinguishing.
According to the embodiment of the invention, 100mg/L of Alxin blue dye liquor is placed in the first solution tank 8, and 100mg/L of alizarin red dye liquor is placed in the second solution tank 11.
After the completion of the bone formation dyeing, the dye solution in the second solution tank 11 is replaced by a transparent solution to soak the sample for 12-24 hours, and after the completion of the soaking, the transparent solution is replaced by 100% glycerol, so that the sample can be stored for a long time.
The cotton thread is a colored cotton thread dyed by reactive dyes commonly used in the market, is convenient to purchase, is easy to obtain, is low in price, saves money, is convenient to operate compared with the conventional method that a small label is tied on the rib at the middle part of the waist and abdomen during specimen preparation, and the adopted colored cotton thread is light and handy, is not easy to fall off and is not easy to break. The method is suitable for liquid exchange in specimen preparation, has good effect by shaking, and the common reactive dye of the colored cotton thread is not affected in the processes of corrosion, dyeing and transparency and is not decolorized; can also be stored in glycerol for a long period without decolorization.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (7)
1. The preparation device of experimental animal embryo bone specimen comprises a plurality of solution boxes, a winding component and a dyeing component arranged in the solution boxes, and is characterized in that,
the plurality of solution boxes are distributed at intervals, at least one dyeing component is arranged in any one of the solution boxes, and different dye solutions are arranged in the plurality of solution boxes;
the dyeing assembly comprises a plurality of clamping blocks which are arranged in parallel, and a sample groove is arranged between any two clamping blocks;
grooves are formed in two ends of the solution tank, supporting mechanisms are arranged on the inner sides of the grooves, and pressing mechanisms are connected to the inner wall of the solution tank in a matched mode;
the winding assembly comprises a first winding roller and a second winding roller, at least one first pull rope is wound on the first winding roller, at least one second pull rope is wound on the second winding roller, and the first pull rope corresponds to the sample groove in position;
a plurality of salient points are arranged on the first pull rope and the second pull rope at intervals, and the first pull rope and the second pull rope can be wound by the first winding roller and the second winding roller respectively;
the pressing mechanisms are in one-to-one correspondence with the sample grooves, and are provided with anti-deflection grooves, and the positions of the anti-deflection grooves correspond to the positions of the sample grooves;
the first pull rope is positioned below the pressing mechanism and is arranged at the inner side of the anti-deflection groove;
the support mechanism is provided with at least one deviation preventing groove, the second pull rope is positioned above the support mechanism, and the second pull rope is positioned at the inner side of the deviation preventing groove;
clamping the marked child in the middle through the first pull rope and the second pull rope, then moving the child in the same direction at the same speed through the first roller and the second roller, and pressing the child into the sample groove through the pressing mechanism when the child is conveyed to the position above the first solution tank; stopping the second stay cord and removing, only driving first stay cord laminating child top layer through first roller and removing, the in-process that first stay cord removed can beat child surface through the bump.
2. The preparation device of experimental animal embryo bone specimens according to claim 1, wherein a chute is arranged in the solution tank, the chute is connected with the pressing mechanism in a matched manner through a sliding mechanism, and the sliding mechanism can slide along the chute.
3. The preparation device of experimental animal embryo bone specimens according to claim 1, wherein a rope groove is arranged between any two clamping blocks, a plurality of adjusting mechanisms are arranged at the bottoms of the clamping blocks, and the adjusting mechanisms can stretch and retract.
4. The preparation device of experimental animal embryo bone specimens according to claim 1, wherein the number of the first winding rollers is two, the number of the second winding rollers is two, the number of the first winding rollers rotates in the same direction at the same speed, the number of the second winding rollers rotates in the same direction at the same speed, and the rotating speeds of the first winding rollers and the second winding rollers can be the same or different.
5. A method for marking experimental animal embryo bone specimens, which is applied to the preparation device of the experimental animal embryo bone specimens according to any one of claims 1-4, and is characterized by comprising the following steps:
s1, removing viscera and fat after euthanasia of a child, opening an abdominal cavity, removing the viscera of a pleuroperitoneal cavity by using ophthalmic forceps, peeling skin by using forceps, and removing fat at the back of the neck and the shoulder blade by using scissors and the ophthalmic forceps;
s2, tying cotton threads with different colors on the waist of the child to identify child with different numbers;
s3, cartilage staining: clamping the child between a first pull rope and a second pull rope by adjusting the rotating speeds of the first winding roller and the second winding roller, transferring the child into a first solution tank, and pressing the child into a sample tank by a pressing mechanism for dyeing for 1-2 days;
s4, osteogenesis staining: after the cartilage is dyed, the height of the sample tank in the solution tank is adjusted by stretching the adjusting mechanism, the young child is clamped and hung through the first pull rope and the second pull rope, meanwhile, the first winding roller and the second winding roller move in a matched mode at the same speed, the young child is transferred to the second solution tank, and the young child is pressed into the sample tank to be dyed for 1 to 3 days through the pressing mechanism.
6. The method according to claim 5, wherein a first pull rope slides along the sample groove during the dyeing process, and the protruding points on the first pull rope are contacted with the fetal bone surface to flap the fetal bone surface.
7. The method for labeling experimental animal fetal calf bone specimen according to claim 5, wherein 100mg/L of allround blue dye solution is placed in the first solution tank, and 100mg/L of alizarin red dye solution is placed in the second solution tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010907715.0A CN112014183B (en) | 2020-09-02 | 2020-09-02 | Preparation device and marking method for experimental animal embryo bone specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010907715.0A CN112014183B (en) | 2020-09-02 | 2020-09-02 | Preparation device and marking method for experimental animal embryo bone specimen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112014183A CN112014183A (en) | 2020-12-01 |
| CN112014183B true CN112014183B (en) | 2024-03-22 |
Family
ID=73515659
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010907715.0A Active CN112014183B (en) | 2020-09-02 | 2020-09-02 | Preparation device and marking method for experimental animal embryo bone specimen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112014183B (en) |
Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09257672A (en) * | 1996-03-22 | 1997-10-03 | Teruaki Ito | Specimen sample and specimen sample preparing apparatus |
| JP2001174376A (en) * | 1999-12-17 | 2001-06-29 | Inst Of Physical & Chemical Res | Laboratory device for organic-tissue slice sample and sample holder |
| EP1160612A1 (en) * | 2000-05-29 | 2001-12-05 | Sulzer Medica AG | Conveyor for specimen slide |
| DE20210451U1 (en) * | 2002-07-05 | 2002-10-17 | Leica Microsystems | Drive for a device for coloring objects |
| JP2002320428A (en) * | 2000-07-05 | 2002-11-05 | Mitsubishi Kasei Institute Of Life Sciences | Method for producing nucleus-transplanted egg, nucleus- transplanted egg obtained by the method, clonal individual obtained by breeding the nucleus- transplanted egg and method for producing the clonal individual |
| WO2007137272A2 (en) * | 2006-05-22 | 2007-11-29 | 10H, Inc. | Device and methods for preparing microscopy samples |
| CN201835124U (en) * | 2010-09-26 | 2011-05-18 | 新昌县艺力机械有限公司 | Continuous cotton dyeing machine |
| CN203101146U (en) * | 2012-12-13 | 2013-07-31 | 中国人民解放军军事医学科学院毒物药物研究所 | Batch dyeing case for foetus skeletons |
| KR101430253B1 (en) * | 2014-05-08 | 2014-09-23 | (주)하나 | Coating method for both sides of split leather and coating apparatus for the same |
| WO2015086531A1 (en) * | 2013-12-13 | 2015-06-18 | Ventana Medical Systems, Inc. | Automated processing systems and methods of thermally processing microscope slides |
| CN105307718A (en) * | 2013-04-19 | 2016-02-03 | 奥库利维公司 | Nasal stimulation devices and methods |
| CN205455615U (en) * | 2016-03-22 | 2016-08-17 | 山东省药学科学院 | Young specimen -box of child |
| KR101684233B1 (en) * | 2015-11-13 | 2016-12-20 | 김정식 | Method for Dyeing Crepe Yarn Space of Several Color and Apparatus thereof |
| CN106442045A (en) * | 2016-08-22 | 2017-02-22 | 李琳 | Full-automatic dyeing slide covering device |
| CN107249575A (en) * | 2014-12-23 | 2017-10-13 | 格赖夫斯瓦尔德恩斯特-莫里茨-阿尔恩特大学 | Pharmaceutical dosage form for being applied to mucous membrane |
| CN107796661A (en) * | 2017-10-30 | 2018-03-13 | 上海聪浮环保科技有限公司 | A kind of sampling sealing device for the automatic rope closing function of floating plate band in assembled |
| CN108193404A (en) * | 2017-12-22 | 2018-06-22 | 汉帛(中国)有限公司 | A kind of jeans processing technology |
| CN109295417A (en) * | 2018-12-04 | 2019-02-01 | 攀枝花学院 | The method of serialization vacuum electron beam plated film |
| CN110629431A (en) * | 2019-10-28 | 2019-12-31 | 徐州兰贵机械科技有限公司 | Multifunctional nylon product processing equipment |
| CN110672607A (en) * | 2019-10-15 | 2020-01-10 | 华中科技大学 | Ex-situ microscopic imaging system in section collection process |
| CN111157320A (en) * | 2020-04-08 | 2020-05-15 | 胜利油田新大管业科技发展有限责任公司 | Fiber yarn tensile sample manufacturing equipment |
| CN211374283U (en) * | 2019-09-16 | 2020-08-28 | 甘肃金域医学检验所有限公司 | Device is got to tissue slide staining rack clamp |
| CN213632875U (en) * | 2020-09-02 | 2021-07-06 | 广东中科英海科技有限公司 | Preparation facilities of young skeleton sample of experimental animals child |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2789952C (en) * | 2010-02-25 | 2018-06-12 | Prairie Ventures, L.L.C. | System and method for anatomical pathology sample handling, storage, and analysis |
| US20140082859A1 (en) * | 2011-12-21 | 2014-03-27 | Shaw Industries Group, Inc. | System and method for space-dyeing yarn |
-
2020
- 2020-09-02 CN CN202010907715.0A patent/CN112014183B/en active Active
Patent Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09257672A (en) * | 1996-03-22 | 1997-10-03 | Teruaki Ito | Specimen sample and specimen sample preparing apparatus |
| JP2001174376A (en) * | 1999-12-17 | 2001-06-29 | Inst Of Physical & Chemical Res | Laboratory device for organic-tissue slice sample and sample holder |
| EP1160612A1 (en) * | 2000-05-29 | 2001-12-05 | Sulzer Medica AG | Conveyor for specimen slide |
| JP2002320428A (en) * | 2000-07-05 | 2002-11-05 | Mitsubishi Kasei Institute Of Life Sciences | Method for producing nucleus-transplanted egg, nucleus- transplanted egg obtained by the method, clonal individual obtained by breeding the nucleus- transplanted egg and method for producing the clonal individual |
| DE20210451U1 (en) * | 2002-07-05 | 2002-10-17 | Leica Microsystems | Drive for a device for coloring objects |
| WO2007137272A2 (en) * | 2006-05-22 | 2007-11-29 | 10H, Inc. | Device and methods for preparing microscopy samples |
| CN201835124U (en) * | 2010-09-26 | 2011-05-18 | 新昌县艺力机械有限公司 | Continuous cotton dyeing machine |
| CN203101146U (en) * | 2012-12-13 | 2013-07-31 | 中国人民解放军军事医学科学院毒物药物研究所 | Batch dyeing case for foetus skeletons |
| CN105307718A (en) * | 2013-04-19 | 2016-02-03 | 奥库利维公司 | Nasal stimulation devices and methods |
| WO2015086531A1 (en) * | 2013-12-13 | 2015-06-18 | Ventana Medical Systems, Inc. | Automated processing systems and methods of thermally processing microscope slides |
| KR101430253B1 (en) * | 2014-05-08 | 2014-09-23 | (주)하나 | Coating method for both sides of split leather and coating apparatus for the same |
| CN107249575A (en) * | 2014-12-23 | 2017-10-13 | 格赖夫斯瓦尔德恩斯特-莫里茨-阿尔恩特大学 | Pharmaceutical dosage form for being applied to mucous membrane |
| KR101684233B1 (en) * | 2015-11-13 | 2016-12-20 | 김정식 | Method for Dyeing Crepe Yarn Space of Several Color and Apparatus thereof |
| CN205455615U (en) * | 2016-03-22 | 2016-08-17 | 山东省药学科学院 | Young specimen -box of child |
| CN106442045A (en) * | 2016-08-22 | 2017-02-22 | 李琳 | Full-automatic dyeing slide covering device |
| CN107796661A (en) * | 2017-10-30 | 2018-03-13 | 上海聪浮环保科技有限公司 | A kind of sampling sealing device for the automatic rope closing function of floating plate band in assembled |
| CN108193404A (en) * | 2017-12-22 | 2018-06-22 | 汉帛(中国)有限公司 | A kind of jeans processing technology |
| CN109295417A (en) * | 2018-12-04 | 2019-02-01 | 攀枝花学院 | The method of serialization vacuum electron beam plated film |
| CN211374283U (en) * | 2019-09-16 | 2020-08-28 | 甘肃金域医学检验所有限公司 | Device is got to tissue slide staining rack clamp |
| CN110672607A (en) * | 2019-10-15 | 2020-01-10 | 华中科技大学 | Ex-situ microscopic imaging system in section collection process |
| CN110629431A (en) * | 2019-10-28 | 2019-12-31 | 徐州兰贵机械科技有限公司 | Multifunctional nylon product processing equipment |
| CN111157320A (en) * | 2020-04-08 | 2020-05-15 | 胜利油田新大管业科技发展有限责任公司 | Fiber yarn tensile sample manufacturing equipment |
| CN213632875U (en) * | 2020-09-02 | 2021-07-06 | 广东中科英海科技有限公司 | Preparation facilities of young skeleton sample of experimental animals child |
Non-Patent Citations (2)
| Title |
|---|
| 可手洗柯根纱的染色工艺;秦玉艳;《印染》;20160531(第09期);第20-22页 * |
| 新型丝织准备设备及工艺(续) 第五章 络筒机;江苏丝绸(04);第44-51页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112014183A (en) | 2020-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112014183B (en) | Preparation device and marking method for experimental animal embryo bone specimen | |
| CN213632875U (en) | Preparation facilities of young skeleton sample of experimental animals child | |
| CN106332870A (en) | Acer palmatum thunb variety acer matsumurae green retention method | |
| CN103411813B (en) | Method for rapidly and efficiently dyeing arbuscular mycorrhizal fungi | |
| CN205337275U (en) | Novel cocoon is plucked to square silkworm cage device | |
| CN212152716U (en) | Full real silk surface fabric reactive dye high colour fastness environmental protection dyeing apparatus | |
| CN107907397A (en) | Collagenous fibres horse pine trichrome stain kit and preparation method thereof and colouring method | |
| CN209432560U (en) | Slide staining apparatus | |
| CN107568198A (en) | The sample for preparing the method for meiofauna pattern of glassy carcass and preparing in this way | |
| CN2881595Y (en) | Dyeing dry plate device used for inspecting biological material | |
| CN102879251B (en) | Dehydrating and dyeing dual-function machine and control method thereof | |
| CN201192671Y (en) | Screw automatic spiral dipping machine | |
| CN213689128U (en) | Novel gram staining device | |
| CN212670034U (en) | Dyeing apparatus for textile processing | |
| CN208334020U (en) | A kind of glass slide batch dyeing apparatus | |
| CN220170683U (en) | Tableware disinfectant detection device | |
| CN202903569U (en) | Dehydrating and dyeing difunctional machine | |
| CN113155563A (en) | Method for preparing scale insect specimen | |
| JPS612758A (en) | Phenylazosulfonic acid or salt | |
| CN218916993U (en) | Tissue slice dyeing machine capable of quantitatively conveying dyeing agent | |
| CN213295774U (en) | Dyeing equipment for polyester thread production | |
| CN217521024U (en) | Portable environment-friendly detection device | |
| CN217499690U (en) | Auxiliary agent dispersion equipment for cloth dyeing | |
| CN86207758U (en) | Specimen slice producing and dyeing apparatus | |
| CN216585644U (en) | Sewing thread dyeing device for dyeing towel uniformly |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |