CN111990544A - Biological feed for blocking and interfering combination of African swine fever virus and specific receptors of pigs and application thereof - Google Patents
Biological feed for blocking and interfering combination of African swine fever virus and specific receptors of pigs and application thereof Download PDFInfo
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- CN111990544A CN111990544A CN202010844117.3A CN202010844117A CN111990544A CN 111990544 A CN111990544 A CN 111990544A CN 202010844117 A CN202010844117 A CN 202010844117A CN 111990544 A CN111990544 A CN 111990544A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a biological feed for blocking and interfering the combination of African swine fever virus and a specific receptor of a pig and application thereof, and the biological feed comprises the following components: 5-10 wt% of a hydrophilic surfactant; 10-20 wt% of a lipophilic surfactant; 40 wt% of montmorillonite; 30-45 wt% of an organic vehicle. The biological feed provided by the invention is added into complete feed, and can block and interfere the combination of the African swine fever virus and a specific virus receptor of a pig, so that the risk of infection of the African swine fever virus is greatly reduced; in addition, the method is simple and easy to implement, low in cost, safe and practical.
Description
Technical Field
The invention belongs to the scientific field of animal biology, and relates to a biological feed for blocking and interfering the combination of African swine fever virus and a specific receptor of a pig and application thereof.
Background
African swine fever is an acute, febrile and highly contagious animal infectious disease, the morbidity and mortality of the African swine fever can reach 100 percent, and the African swine fever is a number one killer in the pig industry, and no commercial vaccine is available at present. The African swine fever virus is the only member of African swine fever virus of African swine fever family, the diameter of the virus particle is 175-215nm, the virus particle is symmetrical in a 20-face body and has an envelope; the genome is double stranded DNA with a size of 170-190 kb. The disease is first reported in kenya in 1921, and since 2007, the African swine fever occurs, spreads and epidemics in Russia and other countries around the world, especially the first African swine fever is diagnosed in 8 months in 2018, and the epidemic situation rapidly spreads to 32 provinces (city, autonomous state) in the whole country; in 2019, African swine fever also occurs in succession in countries such as southeast Asia, and the prevention and control situation is extremely severe.
The African swine fever virus has super-strong viability, and immature meat, salted meat and swill can survive for a long time; the virus can survive for 6 years in the blood in a low-temperature dark room, and can be killed by heating at 56 ℃ for 70 minutes or 60 ℃ for 20 minutes. However, to date, despite the last hundred years of effort of global scientists, no commercial vaccine capable of effectively controlling African swine fever has been developed all over the world, and biosafety is the only effective control means at present.
African Swine Fever (ASFV) is a highly contagious infectious disease that enters the pig body through the mouth and upper respiratory system, and is infected in the nasopharynx or tonsil, and the virus rapidly spreads to the mandibular lymph node, invading the whole body through lymph fluid and blood.
The virus infection of animals depends on the adsorption of virus adsorption protein to animal cell surface receptors, and the interaction of the virus surface specific adsorption protein and the virus receptors on the animal sensitive cell surfaces is the first step of starting virus infection and is a key link for determining the success of the virus infection. The cell receptor of the virus refers to a cell surface site which can generate specific binding with virus adsorption protein, mediate virus invasion into cells and start virus infection. The viral receptors determine the host spectrum of the virus, the avidity of the tissue cells and its pathogenicity.
The African swine fever virus only infects pigs, other animals do not infect, which indicates that there is a specific receptor between the African swine fever virus and the cells of the pig body, therefore, theoretically, only the combination between the African swine fever virus and the receptors of the pigs is blocked, the infection and transmission path of the African swine fever virus can be cut off, and the pigs can be free from infection and keep healthy.
Disclosure of Invention
In order to effectively prevent and reduce the onset risk of the African swine fever, the invention aims to provide a biological feed for blocking and interfering the combination of the African swine fever virus and a specific receptor of a pig and application thereof, which can cut off or interfere the combination between the African swine fever virus and the specific receptor of the pig, and further cut off the infection and transmission path of the African swine fever virus, so that the pig is free from infection and keeps healthy.
In order to achieve the technical purpose, the invention provides a biological feed for blocking and interfering the combination of African swine fever virus and a specific receptor of a pig, which comprises the following components:
preferably, the biological feed for blocking and interfering the binding of the African swine fever virus to the specific receptor of the pig comprises the following components:
preferably, the hydrophilic surfactant is at least one selected from the group consisting of caprylic capric acid glyceride, caprylic acid hexa-polyglyceryl ester, caprylic acid deca-polyglyceryl ester, sodium caseinate, mono-diglycerol fatty acid ester, and monoglyceride.
Preferably, the lipophilic surfactant is selected from at least one of glyceryl monolaurate, glyceryl monooleate, polyglyceryl monooleate, diglyceryl monolaurate, triglyceryl monolaurate, soybean phospholipid, and polyglyceryl stearate.
Preferably, the organic carrier is at least one selected from the group consisting of bran meal, corncob meal and wheat middling.
Preferably, the hydrophilic surfactant is food grade, and the purity is not lower than 99%; the lipophilic surfactant is food grade, and the purity is not lower than 99%; the montmorillonite is feed grade or pharmaceutical grade, and the purity is not lower than 90%.
The raw material components of the invention are all common products which can be purchased conveniently in the market.
The invention also provides application of the biological feed for blocking and interfering the combination of the African swine fever virus and the specific receptors of pigs, and the biological feed is added into complete feed and is uniformly mixed to feed the pigs.
Preferably, the addition amount of the biological feed is 0.5-1 wt% of the complete feed.
The principle of the invention is as follows:
the cell receptor of the virus refers to a cell surface site which can generate specific binding with virus adsorption protein, mediate virus invasion into cells and start virus infection, and determines the host spectrum of the virus, the avidity and pathogenicity of histiocytes. The african swine fever virus only affects pigs, which are the only natural host. The African swine fever virus is a highly contagious disease, and is mainly infected by binding with cell receptors of the African swine fever virus in the nasopharynx part or tonsil part of a pig through mutual contact of feed, drinking water and a pig, and by cutting off and preventing infection and transmission of the African swine fever virus as long as specific binding between the African swine fever virus and the cell surface receptors of the pig is blocked and interfered, as shown in FIG. 1.
The first innovation of the invention is to apply a film to the oral and nasopharyngeal mucosa of the pig to shield the virus receptor, as shown in figure 2. The invention skillfully utilizes that the mucosal surfaces of the oral cavity, the nasopharynx part, the tonsil part and the esophagus (above the stomach) of the pig are all a moist water interface, hydrophilic surfactant is added into the feed, and the pig remains on the mucosal surfaces of the oral cavity, the nasopharynx part, the tonsil and the esophagus (above the stomach) and forms a hydrophilic interface (hydrophobic groups are outward) in the air after ingestion, so that the cell receptors on the mucosal surfaces of the oral cavity, the nasopharynx part, the tonsil part and the esophagus (above the stomach) of the pig are covered (equivalent to the film is attached to the African swine fever cell receptor), and the combination of the cell surface receptor and the virus is blocked and interfered.
The second innovation point of the invention is to apply the film to the African swine fever virus to shield the virus adsorption protein. The interaction of lipid molecules with the virus particles can mask the virus-adsorbed proteins, thereby blocking or blocking their binding to cellular receptors, thus making viral infection difficult to occur. The African swine fever virus is a DNA envelope virus (diameter 175-.
The third innovation of the present invention is to eliminate electrostatic adsorption by using the principle of repulsion, as shown in fig. 4. When entering nasopharynx, tonsil and esophagus through feed ingestion or other ways, African swine fever virus wrapped by lipophilic surfactant repels hydrophilic ionic interface formed by hydrophilic surfactant on the surface of mucosa, and the two cooperate to further obstruct and interfere the combination of virus and specific receptor.
The invention has the beneficial effects that:
(1) the biological feed can block and interfere the combination of the African swine fever virus and a specific virus receptor of a pig, thereby greatly reducing the risk of infection of the African swine fever virus;
(2) the product of the invention is not beneficial to promoting the growth of pigs and improving the utilization rate of feed;
(3) the method is simple and easy to implement, and has low cost;
(4) the hydrophilic surfactant and the lipophilic surfactant added into the biological feed are food additives, and are safe and practical.
Drawings
FIG. 1 is a schematic diagram of the viral infection mechanism of African swine fever virus binding to cell surface virus specific receptors of the mucosa;
FIG. 2 is a schematic view of the principle of applying a film to the mucosa to mask its viral receptors;
FIG. 3 is a schematic diagram of the principle of attaching a membrane to African swine fever virus to shield the protein adsorbed by the virus;
FIG. 4 is a schematic diagram of the principle of the present invention;
FIG. 5 is a blood collection pathogen detection diagram of pigs before and after re-raising, wherein the left diagram is before re-raising, M represents Marker DL3000, negative represents negative control, positive represents positive control, 1-5 is experiment A group, and 6-10 is experiment B group; the right panel shows the result after re-breeding, wherein M represents Marker DL3000, negative represents negative control, positive represents positive control, 1-5 represents experiment A group, 6-8 represents experiment B group, and 9 represents feed.
Detailed Description
The following examples and examples are intended to further illustrate the invention, but are not intended to limit the invention.
Example 1
The biological feed is prepared by the following mass percentages:
example 2
The biological feed is prepared by the following mass percentages:
example 3
The biological feed is prepared by the following mass percentages:
example 4
The biological feed is prepared by the following mass percentages:
example 5
The biological feed is prepared by the following mass percentages:
example 6
And (3) a re-culture test: african swine fever epidemic situation occurs in 8 months in a certain rural farm in the Datong lake area of Yiyang city in this year, and the pigs in the whole farm are subjected to harmless treatment and then are subjected to environmental disinfection. After 9 months, the re-breeding experiment is carried out, 10 sentinel pigs with the weight of 15 kg are selected to be divided into 2 groups: the group A has 5 heads, the group B has 5 heads, and the sentinel pigs are subjected to blood sampling for antigen-antibody detection before the start of re-breeding, and the results show that the introduced pigs are all negative pigs and are infected by African swine fever virus (as shown in the left picture of figure 5). The product of example 2 was then fed as group a with no additions and group B with 1 wt% additions to the full ration. During breeding, group a was reared: after 5 days, the overall feed intake of 5 pigs is low, the mental state is poor, the pigs are emaciated and weak, and some pigs hardly eat the feed; the 5 pigs in the re-breeding group B have good mental state and high feed intake, and no problem pigs are found, and the detailed conditions are shown in Table 1. And (3) on the 8 th day of re-cultivation, 2 deaths of the group A are re-cultivated, and the feed and the serums of sentinel pigs are immediately collected by a farm for etiology detection: the result is shown in the right picture of fig. 5, the pathogen in the fed feed is detected to be positive, and the virus exists; the group A has 4 pigs infected with virus, and the group B has negative serum test and no virus infection. When the pig farm is used, the pig farm is cleaned accurately, 5 pigs of the group A are reared again and are subjected to harmless treatment, and the pig farm is disinfected strictly and takes related biological safety prevention and control measures. The group B was kept in re-breeding for 1 week (day 15), and the pigs were in good mental status, alive and normal feed intake, and the daily feed intake was 1 kg/head. To date, only pigs in group B grew and were in good health.
TABLE 1 repeated culture test of farm in certain village and town of Datong lake region in Yiyang City
| Group of | Number of heads | Weight (kg) | Daily average feed intake (kg) | Number of infected pigs | Number of dead |
| Group A | |||||
| 5 | 15.22 | 0.56 | 4 | 2 | |
| |
5 | 14.96 | 0.84 | 0 | 0 |
Example 7
The product of the invention has the functions of promoting growth and improving feed conversion rate. The bracing structures of the four bracing cities are towards the bracing structures of the four bracing cities and towards a certain pig farm in village 417 pigs, the African swine fever epidemic situation around the farm in 8 months in 2019 is serious, and the African swine fever epidemic situation occurs in an adjacent pig farm 300 meters away from the farm. On day 8, month 11, the pig farm was disinfected of the surrounding environment and the product of example 2 was fed to the herd at 0.5 wt% with the feed. After the product is used for 15 days (till 8 months and 26 days), no suspicious cases occur in the pig farm, the health condition of the pig herd is good, and no other epidemic diseases occur. As can be seen from the data in the following table 2, the daily gain of the experimental group of swinery is increased by about 10% compared with that of the control group, and the phase feed weight ratio is lower than that of the control group, which indicates that the product of the example 2 is added into the feed, so that the palatability is better, and the growth performance of the swinery can be improved.
TABLE 2 bracing structures in four corners of market and bracing structure in certain pig farm test
Example 8
Accurately eliminating and purifying African swine fever. According to the four corners in the four cities, 109 sows, 112 weaned piglets, 78 nursery pigs and 120 fattening pigs are fed in one pig farm in the village and the rest village, the African swine fever epidemic situation occurs in one fattening pig house in 8 months and early in 2019, 10 pigs die in two days, emergency prevention and control measures are taken immediately in the pig farm, and strict biological safety prevention and control are carried out in the whole farm. From 8 months and 11 days, the product of the example 2 is used in the whole pig farm, the feed is mixed according to the proportion of 1 wt% to feed the pig herd for 15 days, and then the feed is adjusted to be 0.5 wt% for addition; 128 pigs in one nursery pig house are reserved, namely 50 pigs in the group A are fed by mixing materials according to the proportion of 0.5 wt%, and 78 pigs in the group B are fed by mixing materials according to the proportion of 1 wt%, so that observation of different addition effects is carried out. The results show that: until now (10 months and 10 days), no suspicious cases occur in the whole field, which shows that the non-plague spreading is successfully and accurately eliminated and blocked, and the morbidity of respiratory tract, intestinal tract and bacterial diseases of the swinery is extremely low; the sow farrowing is normal, and the survival rate of the weaned pig is 98%. Comparative experiment of the addition amount of nursery pigs: the daily gain of the group B (1 wt%) was increased by 4% compared with the group A (0.5 wt%), the phase material weight ratio was also lower compared with the group A, and there was no difference in the incidence. Comprehensively considered, the product of the invention with the addition of 0.5-1 wt% can accurately eliminate and block the non-pestilence transmission by combining with the traditional biological safety measures such as disinfection and the like, and the growth performance of the swinery is better by adding 1 wt%. Specific data are detailed in table 3 below:
table 3 bracing structure of Liuyang city and certain pig farm test of Chongzhen and village
| Group of | Number of heads | Weight (kg) | Average weight of powder (kg) | Daily gain (g) | Number of onset of disease | Number of dead heads | Material to weight ratio |
| A(0.5％) | 50 | 34.8 | 45.3 | 350 | 0 | 0 | 2.2 |
| B(1％) | 78 | 33.1 | 44 | 363 | 0 | 0 | 2.15 |
Claims (8)
1. A biological feed for blocking and interfering the combination of African swine fever virus and a specific receptor of a pig is characterized by comprising the following components:
2. the biological feed for blocking and interfering with binding of african swine fever virus to a specific receptor of a pig according to claim 1, wherein: the biological feed for blocking and interfering the combination of the African swine fever virus and a specific receptor of a pig comprises the following components:
3. the biological feed for blocking and interfering with the binding of african swine fever virus to a specific receptor of a pig according to claim 1 or 2, wherein: the hydrophilic surfactant is at least one selected from caprylic capric acid glyceride, caprylic acid hexaglycerol, caprylic acid decaglycerol, sodium caseinate, mono-diglycerol fatty acid ester and monoglyceride.
4. The biological feed for blocking and interfering with the binding of african swine fever virus to a specific receptor of a pig according to claim 1 or 2, wherein: the lipophilic surfactant is at least one selected from the group consisting of glycerol monolaurate, glycerol monooleate, polyglycerol monooleate, diglycerol monolaurate, triglycerol monolaurate, soybean phospholipid and polyglycerol stearate.
5. The biological feed for blocking and interfering with the binding of african swine fever virus to a specific receptor of a pig according to claim 1 or 2, wherein: the organic carrier is at least one of bran meal, corncob meal and wheat middling.
6. The biological feed for blocking and interfering with binding of African swine fever virus to a specific receptor of a pig according to claim 3, wherein: the hydrophilic surfactant is food grade, and the purity is not lower than 99%; the lipophilic surfactant is food grade, and the purity is not lower than 99%; the montmorillonite is feed grade or pharmaceutical grade, and the purity is not lower than 90%.
7. Use of the biological feed according to any of claims 1 to 6 for blocking and interfering with the binding of African Swine fever Virus to specific receptors of pigs, characterized in that: the feed additive is added into complete feed and is uniformly mixed to feed the live pigs.
8. The use of the biological feed for blocking and interfering with the binding of African swine fever virus to a specific receptor of a pig according to claim 7, characterized in that: the addition amount of the biological feed is 0.5-1 wt% of the complete feed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2019109828689 | 2019-10-16 | ||
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105124301A (en) * | 2015-09-22 | 2015-12-09 | 武汉轻工大学 | Fat composition for reducing immunological stress of pigs and application of fat composition |
| CN106166160A (en) * | 2011-09-16 | 2016-11-30 | 吉利德制药有限责任公司 | For treating the compositions of HCV |
| CN106234770A (en) * | 2016-07-28 | 2016-12-21 | 湖南晶天科技实业有限公司 | A kind of feed additive, its application and a kind of feedstuff |
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- 2020-08-20 CN CN202010844117.3A patent/CN111990544A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106166160A (en) * | 2011-09-16 | 2016-11-30 | 吉利德制药有限责任公司 | For treating the compositions of HCV |
| CN105124301A (en) * | 2015-09-22 | 2015-12-09 | 武汉轻工大学 | Fat composition for reducing immunological stress of pigs and application of fat composition |
| CN106234770A (en) * | 2016-07-28 | 2016-12-21 | 湖南晶天科技实业有限公司 | A kind of feed additive, its application and a kind of feedstuff |
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Effective date of registration: 20230522 Address after: Room 304, 3rd Floor, Building A, No. 618 Heping Road, Longping High tech Park, Furong District, Changsha City, Hunan Province, China (Hunan) Free Trade Pilot Zone, 410116 Applicant after: CHANGSHA LVYE BIOTECHNOLOGY CO.,LTD. Address before: 410125 Hunan province Changsha Furong district Mapoling Academy of Agricultural Sciences in Applicant before: CHANGSHA LVYE BIOTECHNOLOGY CO.,LTD. Applicant before: HUNAN YANCUN VILLAGE ECOLOGICAL AGRICULTURE & HUSBANDRY TECHNOLOGY CO.,LTD. |