CN111982631B - Pathological tissue dewatering device - Google Patents
Pathological tissue dewatering device Download PDFInfo
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- CN111982631B CN111982631B CN202010876728.6A CN202010876728A CN111982631B CN 111982631 B CN111982631 B CN 111982631B CN 202010876728 A CN202010876728 A CN 202010876728A CN 111982631 B CN111982631 B CN 111982631B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a pathological tissue dehydration device, and belongs to the technical field of tissue treatment equipment. This dewatering device includes: the tissue dehydration tank is used for storing pathological tissues to be dehydrated; the first atomization device is used for atomizing dehydrating agents with different concentrations into small molecular liquid beads; the inert gas supply device is communicated with the first atomization device, and the mixed gas-liquid mixture is conveyed into the tissue dehydration tank through a conveying pipe; the tissue dehydration tank is also connected with an output pipe used for conveying the gas-liquid mixture in the tissue dehydration tank to the buffer tank; the output pipe is also provided with a first humidity sensor for detecting the humidity of the output gas in the tissue dehydration tank. The dehydrating device effectively solves the problems of low tissue dehydrating efficiency and incomplete dehydrating, simultaneously has less dehydrating agent dosage, recycles the dehydrating agent, reduces the treatment capacity of the dehydrating agent after use, and can realize the purposes of dehydrating a plurality of tissues and improving the dehydrating efficiency without increasing the dosage of the dehydrating agent.
Description
Technical Field
The invention relates to the technical field of tissue treatment equipment, in particular to a pathological tissue dehydration device.
Background
The method is characterized in that water contained in pathological tissue affects the dipping and filling of embedding paraffin on denatured protein fibers, a tissue dehydrating agent is required to be used for completely dissolving and removing, in the tissue dehydrating process, the fixed tissue intracellular water and the tissue dehydrating agent are mutually soaked and combined, when the tissue dehydrating agent is mutually dissolved and balanced, the tissue dehydrating agent extracts the tissue intracellular water to reach the mutual dissolving and balancing and the concentration is almost unchanged, the tissue intracellular water is completely dissolved and removed, in order to avoid serious deformation and excessive hardening caused by the fact that a tissue block is directly soaked in high-concentration ethanol for dehydration too fast, ethanol with different concentrations is used for continuously soaking the tissue for dehydration, therefore, a plurality of reagent tanks with dehydrating agents with different concentrations are generally required to be prepared by a case tissue dehydrating device in the prior art, then the tissue is sequentially placed into each reagent tank for soaking until the water content of the pathological tissue is not more than 5 percent, and then the method is finished. The traditional pathological tissue dehydration device has the problems that the dosage of a dehydrating agent is large, the replacement of the dehydrating agent and water completely depends on the free permeation of a solvent, the replacement efficiency is low, and the replacement is incomplete.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide a pathological tissue dehydration device.
The invention provides a pathological tissue dehydration device, comprising:
the tissue dehydration tank is used for storing pathological tissues to be dehydrated, and the top of the tissue dehydration tank is provided with a sealing cover;
the first atomization device is connected with the dehydrating agent supply device and is used for atomizing the dehydrating agent with different concentrations into small molecular liquid beads;
the inert gas supply device is communicated with the first atomization device and is used for supplying dry inert gas mixed with the dehydrating agent microbeads, and the mixed gas-liquid mixture is conveyed into the tissue dehydration tank through the conveying pipe;
the tissue dehydration jar still is connected with the output tube that is used for carrying its inside gas-liquid mixture to the buffer memory case, still be equipped with the first humidity transducer that is used for detecting the humidity of output gas in the tissue dehydration jar on the output tube.
Preferably, the buffer box is communicated with the first atomization device and used for increasing the content of a dehydrating agent in the buffer box, the buffer box is further connected with the conveying pipe through a circulating pump, a second humidity sensor used for detecting the water content of gas entering the tissue dehydration tank is arranged on the conveying pipe, a dehydrating agent content detection device is arranged in the buffer box, the first humidity sensor and the second humidity sensor are electrically connected with the controller, and the controller is further electrically connected with a signal indication lamp arranged on the tissue dehydration tank.
Preferably, the number of the tissue dewatering tanks is multiple, the tissue dewatering tanks are sequentially arranged, each tissue dewatering tank is provided with an input port and an output port, the output port of each tissue dewatering tank is connected with the input port of the next tissue dewatering tank through a connecting pipe, the input port of the first tissue dewatering tank is connected with a conveying pipe, the output end of the tissue dewatering tank at the tail end is connected with an output pipe, each connecting pipe is provided with a third humidity sensor, and the third humidity sensor is electrically connected with the controller.
Preferably, each connecting pipe is provided with a third humidity sensor, and the third humidity sensor is also electrically connected with the controller.
Preferably, each connecting pipe and each output pipe are provided with a sterilizing device.
Preferably, the tissue dehydration jar is further provided with a pressure sensor for detecting the internal pressure of the tissue dehydration jar, the output pipe is provided with an electromagnetic valve, and the pressure sensor and the electromagnetic valve are in signal connection with the controller.
Preferably, the tissue dewatering tank comprises an inner tank body and an outer tank body, the inner tank body is arranged in the outer tank body, a gap is reserved between the inner tank body and the outer tank body, a plurality of air holes are formed in the inner tank body, a rotary disc is arranged on the inner top of the sealing cover, a tissue basket used for storing case tissues is arranged in the inner tank body, the tissue basket is connected with the bottom of the rotary disc through a telescopic rod, the sealing cover is connected with the outer tank body in a sealing mode, and the air outlet end of the conveying pipe is located in the gap between the inner tank body and the outer tank body.
Preferably, the bottom of the tissue dehydration tank is further provided with a liquid collecting tank, and a second atomization device is arranged in the liquid collecting tank and used for atomizing the liquid collected by the tissue dehydration tank.
Preferably, the inert gas is one of argon or nitrogen.
Preferably, the output pipe is further provided with a water removal device, and the water removal device comprises a shell and a water absorbent filled in the shell.
Compared with the prior art, the invention has the beneficial effects that: the dehydrating device provided by the invention atomizes the dehydrating agent into tiny liquid beads, then mixes the tiny liquid beads with the inert gas, carries the dehydrating agent liquid beads with the inert gas to enter the tissue dehydrating tank, the liquid beads enter the tank body through the through holes arranged on the inner tank body, so that the gas-liquid mixed gas can be rapidly and uniformly contacted with the pathological tissue to be dehydrated, dehydrating agent microbeads in the gas-liquid mixed gas can be attached to the surface of the pathological tissue and dissolve and remove water in the tissue, and the dehydrating agent can rapidly and uniformly permeate into the tissue under the drive of the inert gas to replace the water in the tissue.
The invention also utilizes the second atomization device to atomize the mixed liquid in the tissue dehydration tank, discharges the mixed liquid along with the inert gas, simultaneously detects the water content in the gas-liquid mixed gas before and after entering the tissue dehydration tank, and when the water content before and after entering the tissue dehydration tank is kept unchanged within a certain time, the dehydration is complete, and the signal indicator lamp lights up to send out a reminding signal of the dehydration completion.
The tissue dehydrating tanks are arranged, so that the simultaneous dehydration of a plurality of tissues is met, the dehydrating agents are maximally utilized in a series connection mode among the tissue dehydrating tanks, the tissues are dehydrated without increasing the using amount of the dehydrating agents, and the dehydration efficiency is improved.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of embodiment 2;
FIG. 3 is a schematic view of embodiment 3;
FIG. 4 is a schematic structural view of a liquid collecting tank and a second atomization device on the inner tank body of the invention;
figure 5 is a schematic view of the structure of the tissue dewatering tank of the present invention.
Description of reference numerals:
1. the device comprises a tissue dehydration tank, a sealing cover 101, a sealing cover 102, an inner tank body 103, an outer tank body 104, air holes, a rotating disc 105, a tissue basket 106, a telescopic rod 107, a first atomization device 2, an inert gas supply device 3, a conveying pipe 4, an output pipe 5, a buffer tank 6, a first humidity sensor 7, a connecting pipe 8, a circulating pump 9, a content detection device 10, a dehydrating agent tank 11, a dehydrating agent tank 12, a second atomization device 13, a signal indicator lamp 14 and a water adsorption device 14.
Detailed Description
Detailed description of the preferred embodimentsthe following detailed description of the present invention will be made with reference to the accompanying drawings 1-5, although it should be understood that the scope of the present invention is not limited to the specific embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
The embodiment provides a pathological tissue dehydration device, includes:
the tissue dehydration tank 1 is used for storing pathological tissues to be dehydrated, and the top of the tissue dehydration tank is provided with a sealing cover 101;
the first atomization device 2 is connected with the dehydrating agent supply device and is used for atomizing dehydrating agents with different concentrations into small molecular liquid beads;
the inert gas supply device 3 is communicated with the first atomization device 2 and is used for supplying dry inert gas mixed with the dehydrating agent microbeads, and the mixed gas-liquid mixture is conveyed into the tissue dehydration tank 1 through the conveying pipe 4;
the tissue dehydration tank 1 is also connected with an output tube 5 for conveying the gas-liquid mixture in the tissue dehydration tank to the buffer tank 6, and the output tube 5 is also provided with a first humidity sensor 7 for detecting the humidity of the output gas in the tissue dehydration tank 1.
The dehydrating agent is one of ethanol or acetone, preferably ethanol, the inert gas is preferably nitrogen, and a valve can be arranged on the conveying pipe 4 to better control whether the inert gas or the mixed gas of the inert gas and the dehydrating agent microbeads is required to be conveyed to the tissue dehydrating tank 1 according to the requirement.
The dewatering principle of the dewatering device of the embodiment is as follows: the dehydrating agents with different concentrations are atomized according to concentration gradient and then mixed with inert gas, the mixed gas-liquid mixed gas is conveyed into the tissue dehydration tank 1 to be contacted with the tissue to be dehydrated, dehydrating agent microbeads in the gas-liquid mixed gas can be uniformly distributed on the surface of the tissue to be dehydrated, the water in tissue cells and the tissue dehydrating agents are mutually soaked, gas-liquid circulation is continuously carried out, the concentration of the dehydrating agents is gradually increased until the water in the tissue cells is completely dissolved and removed.
The action of the inert gas in the embodiment is that the inert gas is used as a power source to carry the dehydrating agent microbeads to circularly flow, the inert gas does not react with the tissue, the inert gas can enable the dehydrating agent microbeads to have good dispersibility, the dehydrating agent microbeads are favorably and uniformly attached to the tissue to be dehydrated, and the dehydrating agent can be more easily permeated into the pathological tissue due to the carrying of the gas.
In the embodiment, the humidity of the gas after the gas is discharged from the tissue dehydrating tank 1 is detected by the first humidity sensor 7, the moisture in the gas entering the tissue dehydrating tank 1 completely comes from the dehydrating agent, the moisture content of the gas entering the tissue dehydrating tank 1 and discharged from the tissue dehydrating tank 1 can be determined according to the content of the dehydrating agent, and the moisture content of the gas entering the tissue dehydrating tank 1 and the gas discharged from the tissue dehydrating tank 1 can be considered to be completely dehydrated when the moisture content of the gas entering and discharging is kept consistent within 2-3 min.
When the device is used, inert gas is firstly introduced into the tissue dehydration tank 1, the air in the tissue dehydration tank 1 is discharged, then the dehydrating agent with the initial concentration is added into the first atomization device 2, atomization is carried out through the first atomization device 2, the inert gas supply device 3 is started to supply the inert gas into the first atomization device 2, and the atomized dehydrating agent microbeads and the inert gas are mixed and then enter the tissue dehydration tank 1 through the conveying pipe 4. The volume ratio of the inert gas to the dehydrating agent microbeads is preferably controlled to be 2.5-3, the flowing speed of the dehydrating agent microbeads and the content of the dehydrating agent microbeads in the inert gas can be considered, a gas-liquid mixture coming out of the tissue dehydration tank 1 is collected in the buffer tank 6 through the output pipe 5, gas-liquid separation treatment is carried out on the gas in the buffer tank 6 through the later stage, and the dehydrating agent and the inert gas are recovered.
Example 2
In order to realize the cyclic utilization to the dehydrating agent, the buffer tank 6 of this embodiment is used for increasing the content of the dehydrating agent in the buffer tank 6 with the 2 intercommunications of first atomizing device, and buffer tank 6 still is connected with conveyer pipe 4 through circulating pump 9, be equipped with the second humidity transducer who is used for detecting the gaseous water content in getting into tissue dehydration jar 1 on conveyer pipe 4, be equipped with dehydrating agent content detection device 10 in the buffer tank 6, first humidity transducer 7 and second humidity transducer all are connected with the controller electricity, the controller still is connected with signal indicator 13 electricity that sets up on tissue dehydration jar 1.
Because the water content of the gas-liquid mixture of output tube 5 output is continuous increasing, and the concentration of dehydrating agent needs continuous increasing, consequently with buffer tank 6 and 2 intercommunications of first atomizing device, continuous supplementary dehydrating agent, and detect the dehydrating agent content to buffer tank 6 exit, detect the gas water content that gets into in the tissue dewatering tank 1 through second humidity transducer simultaneously, the adjustment gets into the concentration of the dehydrating agent in the tissue dewatering tank 1, the change condition of water content is known through the detected value of comparing second humidity transducer and first humidity transducer 7, treat that the detected value between them is when unchangeable basically in a period of time, then explain the dehydration completely.
Example 3
In order to satisfy a large amount of pathological tissue's dehydration demand, the quantity of tissue dehydration jar 1 is a plurality of in this embodiment, and a plurality of tissue dehydration jar 1 arrange in proper order, all be equipped with input port and delivery outlet on each tissue dehydration jar 1, the delivery outlet of each tissue dehydration jar 1 all is connected with the input port of next tissue dehydration jar 1 through connecting pipe 8, the first input port of tissue dehydration jar 1 is connected with conveyer pipe 4, the output that is located terminal tissue dehydration jar 1 is connected with output tube 5, all be equipped with third humidity transducer on each connecting pipe 8, third humidity transducer and controller electricity are connected.
This embodiment adopts and establishes ties a plurality of tissue dehydration jar 1 in proper order, then makes gas-liquid mixture gas get into every tissue dehydration jar in proper order, and whether the gas water content that gets into 1 business turn over of every tissue dehydration jar through detecting detects the dehydration completely, treats the dehydration complete back, and the signal indicator lamp that corresponds is glittering, and the staff will be rather than the delivery outlet of adjacent last tissue dehydration jar 1 after taking out with it with next tissue dehydration jar 1's input port connection can. It should be noted that the valve provided in the transfer pipe 4 is used to temporarily close the valve to stop the gas transfer when the tissue dehydrating tank 1 after the dehydration is removed, and to open the valve to resume the gas transfer after the treatment.
Wherein, each connecting pipe 8 and each output pipe 5 are provided with a sterilizing device. The aim is to avoid the mutual influence between the tissues of the tissue dewatering pots 1.
Example 4
As another preferred embodiment, the tissue dehydrating tank 1 of the present embodiment is further provided with a pressure sensor for detecting the internal pressure of the tissue dehydrating tank 1, the output pipes 5 are provided with electromagnetic valves, and the pressure sensor and the electromagnetic valves are in signal connection with the controller. The purpose lies in through keeping certain pressure in making tissue drain sump 1 be inside the infiltration entering tissue that the dehydrating agent can be better, improve dehydration efficiency and dewater more completely.
Example 5
In order to enable gas entering the tissue dehydration tank 1 to be rapidly and uniformly attached to the tissue surface, the tissue dehydration tank 1 of the embodiment comprises an inner tank body 102 and an outer tank body 103, the inner tank body 102 is arranged inside the outer tank body 103, a gap is reserved between the inner tank body 102 and the outer tank body 103, a plurality of air holes 104 are formed in the inner tank body 101, a rotating disc 105 is arranged on the inner top of the sealing cover 101, a tissue basket 106 used for storing case tissues is arranged in the inner tank body 102, the tissue basket 106 is connected with the bottom of the rotating disc 105 through an expansion link 107, the sealing cover 101 is connected with the outer tank body 103 in a sealing manner, and the air outlet end of the conveying pipe 4 is located in the gap between the inner tank body 102 and the outer tank body 103.
In the above embodiment, the dehydrating agent is completely contacted with the tissue by the rotation of the tissue to be dehydrated and the uniform distribution of the airflow, and the large liquid beads attached to the surface of the tissue are separated from the tissue along with the tangential force generated between the rotation of the tissue and the airflow and the centrifugal effect generated during the rotation, so that the problem of reducing the concentration of the dehydrating agent and the dehydration efficiency due to the fact that a large amount of moisture is attached to the surface of the tissue is solved.
Wherein, 1 bottom of tissue dehydration jar still is equipped with collecting tank 11, be equipped with second atomizing device 12 in the collecting tank 11 for the liquid of collecting 1 of tissue dehydration jar atomizes. Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A pathological tissue dehydration device is characterized by comprising
The tissue dehydration tank (1) is used for storing pathological tissues to be dehydrated, and the top of the tissue dehydration tank is provided with a sealing cover (101);
the first atomization device (2) is connected with the dehydrating agent supply device and is used for atomizing the dehydrating agent with different concentrations into small molecular liquid beads;
the inert gas supply device (3) is communicated with the first atomization device (2) and is used for supplying dry inert gas mixed with the dehydrating agent microbeads, and the mixed gas-liquid mixture is conveyed into the tissue dehydration tank (1) through the conveying pipe (4);
the tissue dehydration tank (1) is also connected with an output pipe (5) used for conveying the gas-liquid mixture in the tissue dehydration tank to the buffer tank (6), and the output pipe (5) is also provided with a first humidity sensor (7) used for detecting the humidity of the output gas in the tissue dehydration tank (1);
the tissue dehydration tank is characterized in that the buffer tank (6) is communicated with the first atomization device (2) and used for increasing the content of a dehydrating agent in the buffer tank (6), the buffer tank (6) is further connected with the conveying pipe (4) through a circulating pump (9), a second humidity sensor used for detecting the water content of gas entering the tissue dehydration tank (1) is arranged on the conveying pipe (4), a dehydrating agent content detection device (10) is arranged in the buffer tank (6), the first humidity sensor (7) and the second humidity sensor are both electrically connected with a controller, and the controller is further electrically connected with a signal indicator lamp (13) arranged on the tissue dehydration tank (1);
the quantity of tissue dehydration jar (1) is a plurality of, and a plurality of tissue dehydration jar (1) arrange in proper order, all be equipped with input port and delivery outlet on each tissue dehydration jar (1), the delivery outlet of each tissue dehydration jar (1) all is connected through the input port of connecting pipe (8) with next tissue dehydration jar (1), the input port and the conveyer pipe (4) of first tissue dehydration jar (1) are connected, the output that is located terminal tissue dehydration jar (1) is connected with output tube (5), all be equipped with third humidity transducer on each connecting pipe (8), third humidity transducer and controller electricity are connected.
2. The pathological tissue dehydration device of claim 1 characterized in that each connecting tube (8) and each output tube (5) are equipped with sterilization means.
3. The pathological tissue dehydration device of claim 1, characterized in that said tissue dehydration tank (1) is further equipped with a pressure sensor for detecting the internal pressure of tissue dehydration tank (1), and the output pipes (5) are equipped with electromagnetic valves, both of said pressure sensor and electromagnetic valves are connected with controller by signal.
4. The pathological tissue dehydration device according to claim 1, characterized in that said tissue dehydration tank (1) comprises an inner tank (102) and an outer tank (103), said inner tank (102) is disposed inside the outer tank (103), and a gap is left between the inner tank (102) and the outer tank (103), said inner tank (102) is provided with a plurality of air vents (104), the inner top of said sealing cover (101) is provided with a rotating disc (105), said inner tank (102) is provided with a tissue basket (106) for storing pathological tissues, said tissue basket (106) is connected with the bottom of the rotating disc (105) through a telescopic rod (107), said sealing cover (101) is connected with the outer tank (103) in a sealing manner, and the air outlet end of said delivery pipe (4) is located in the gap between the inner tank (102) and the outer tank (103).
5. Pathological tissue dehydration device according to claim 1, characterized in that said tissue dehydration tank (1) is further provided with a liquid collection tank (11) at the bottom, said liquid collection tank (11) being provided with a second atomization means (12) for atomizing the liquid collected by the tissue dehydration tank (1).
6. The pathological tissue dehydration device of claim 1 wherein said inert gas is one of argon or nitrogen.
7. The pathological tissue dehydration device of claim 1, characterized in that said output pipe (5) is further provided with a water removal device (14), said water removal device (14) comprises a housing and a water absorbent filled in the housing.
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| CN116296682A (en) * | 2023-03-23 | 2023-06-23 | 南京市妇幼保健院 | Totally-enclosed pathological biological tissue dehydrator and application method thereof |
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