CN111972565A - Compound polysaccharide feed additive for improving quality of cock semen and preparation method and application thereof - Google Patents
Compound polysaccharide feed additive for improving quality of cock semen and preparation method and application thereof Download PDFInfo
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- CN111972565A CN111972565A CN202010857431.5A CN202010857431A CN111972565A CN 111972565 A CN111972565 A CN 111972565A CN 202010857431 A CN202010857431 A CN 202010857431A CN 111972565 A CN111972565 A CN 111972565A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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Abstract
The invention belongs to the technical field of animal feed, and particularly discloses a compound polysaccharide feed additive for improving the quality of cock semen, a preparation method and an application thereof, wherein the compound polysaccharide feed additive for improving the quality of cock semen is obtained by mixing agaricus blazei polysaccharide, lycium barbarum polysaccharide, seaweed selenium polysaccharide and a compound polysaccharide stabilizer; the compound polysaccharide feed additive for improving the quality of the semen of the cocks is added into basic ration of the cocks. The compound polysaccharide feed additive for improving the sperm quality of the cocks can improve the sperm quality and the oxidation resistance level of the cocks. The preparation method is suitable for preparing the compound polysaccharide feed additive for improving the quality of the semen of the cocks, and the compound polysaccharide feed additive for improving the quality of the semen of the cocks is suitable for being added into basic daily ration of the cocks to feed the cocks.
Description
Technical Field
The invention belongs to the technical field of animal feed, and relates to an additive, in particular to a compound polysaccharide feed additive for improving the quality of cock semen, and a preparation method and application thereof.
Background
The modern chicken raising mode of high density and intensification enables the chicken raising industry to be rapidly developed. For a long time, the nutrition research on breeding hens is mainly focused on the nutrition of breeding hens, while the nutrition research on breeding cocks is relatively less. With the large-scale application of the cage-breeding mode and the artificial insemination mode, the function of the breeding cock in the healthy development of the poultry industry is more important. Due to the fact that the requirement of the breeding cock semen is increased and the semen collection frequency is increased, the semen quality is poor, the fertility rate and the hatching rate of hatching eggs are reduced, meanwhile, the breeding cock is eliminated in advance, and the healthy development of the chicken industry is affected.
The improvement of the quality of cock semen through various ways is one of the hot spots of the research in the field of animal science at present, the feed nutrition regulation and control is a key factor for obtaining high-quality semen, however, most of the current research results are difficult to popularize and apply in the actual production due to the reasons of poor effectiveness or short effect, and the like, so that the appropriate feed additive with good effectiveness is sought and applied to the production practice to improve the reproductive performance of cocks, and the feed additive has important economic value.
Disclosure of Invention
The invention aims to provide a compound polysaccharide feed additive for improving the quality of cock semen, so as to improve the quality of the cock semen and have good effectiveness; the invention also aims to provide a preparation method of the compound polysaccharide feed additive for improving the quality of the cock semen; the invention also aims to provide the application of the compound polysaccharide feed additive for improving the quality of the cock semen.
In order to achieve the purpose, the invention adopts the following technical scheme:
a compound polysaccharide feed additive for improving the quality of cock semen comprises the following raw materials of effective components by weight ratio of 75-90: 8.5-23.5: 1: 0.5 of agaricus blazei murill polysaccharide, lycium barbarum polysaccharide, seaweed selenium polysaccharide and compound polysaccharide stabilizer.
As a limitation: the selenium content in the seaweed selenium polysaccharide is 10-20 mg/g.
As a further limitation: the raw materials for preparing the effective components of the compound polysaccharide stabilizer comprise 40 percent of potassium sorbate, 30 percent of tocopherol and 30 percent of tea polyphenol by weight percentage.
The invention also provides a preparation method of the compound polysaccharide feed additive for improving the quality of cock semen, which comprises the following steps:
s1, mixing potassium sorbate, tocopherol and tea polyphenol uniformly to prepare a compound polysaccharide stabilizer;
s2, uniformly mixing the agaricus blazei polysaccharide, the lycium barbarum polysaccharide, the seaweed selenium polysaccharide and the compound polysaccharide stabilizer prepared in the step S1 to obtain the compound polysaccharide feed additive for improving the quality of the cock semen.
The invention also provides an application of the compound polysaccharide feed additive for improving the quality of the semen of the cocks, and the compound polysaccharide feed additive for improving the quality of the semen of the cocks is added into basic ration of the cocks to feed the cocks.
As a limitation: the compound polysaccharide feed additive for improving the quality of the semen of the cocks is added into the basic ration of the cocks according to the addition amount of 0.1-0.5 percent of the weight proportion.
The compound polysaccharide feed additive for improving the quality of cock semen comprises the following components:
the agaricus blazei polysaccharide is an effective active ingredient extracted from agaricus blazei fruiting bodies, is fragrant in smell, and has the effects of inhibiting growth of tumor cells, reducing blood sugar, resisting oxidation and radiation, improving immunity, reducing cholesterol and improving arteriosclerosis; it has effects of preventing diseases, improving intestinal flora, and promoting growth;
the lycium barbarum polysaccharide is plant polysaccharide consisting of arabinose, rhamnose, xylose, mannose, galactose, glucose neutral monosaccharide, galacturonic acid and 18 amino acids, and has good functions of regulating immunity, reducing blood sugar, resisting oxidation, delaying aging, promoting tumor cell apoptosis, resisting radiation damage, reducing blood fat and the like; has the functions of regulating immunity, improving carcass quality, promoting growth and the like in animal production;
the seaweed selenium polysaccharide is a polysaccharide which can simultaneously play double roles of seaweed polysaccharide and organic selenium, and has the effects of resisting oxidation, regulating immunity, improving the quality of animal products, promoting growth and the like in animal production.
Due to the adoption of the scheme, compared with the prior art, the invention has the beneficial effects that:
the compound polysaccharide feed additive for improving the quality of the cock semen relieves the stress in poultry bodies, improves the secretion function in the poultry bodies, improves the sperm motility and the sperm density, reduces the sperm aberration rate, and improves the glutathione peroxidase activity in seminal plasma by the compatibility of the agaricus blazei polysaccharide, the lycium barbarum polysaccharide and the seaweed selenium polysaccharide, thereby achieving the effect of improving the quality of the cock semen and further improving the reproductive performance of the cock; the compound polysaccharide feed additive for improving the quality of the cock semen is also added with a compound polysaccharide stabilizer, so that the storage life of the compound polysaccharide feed additive can be effectively prolonged.
In conclusion, the compound polysaccharide feed additive for improving the sperm quality of the cocks improves the sperm quality and the oxidation resistance level of the cocks.
The preparation method is suitable for preparing the compound polysaccharide feed additive for improving the quality of the semen of the cocks, and the compound polysaccharide feed additive for improving the quality of the semen of the cocks is suitable for being added into basic daily ration of the cocks to feed the cocks.
Detailed Description
The present invention is further described with reference to the following examples, but it should be understood by those skilled in the art that the present invention is not limited to the following examples, and any modifications and variations based on the specific examples of the present invention are within the scope of the claims of the present invention.
Examples 1-6 Compound polysaccharide feed additive for improving sperm quality of rooster
Examples 1 to 6 are a compound polysaccharide feed additive for improving the quality of the semen of a cock, and the composition of the raw materials for preparing the compound polysaccharide stabilizer is shown in table 1, and the composition of the raw materials for preparing the compound polysaccharide feed additive for improving the quality of the semen of the cock is shown in table 2.
TABLE 1 EXAMPLES 1-6 raw materials for Compound polysaccharide stabilizers
TABLE 2 examples 1-6 raw materials of compound polysaccharide feed additive for improving sperm quality of cock
In the examples 1-6, the selenium content in the seaweed selenium polysaccharide is respectively 12g, 20g, 9g, 14g, 20g and 32g in sequence.
Examples 1-6 methods for preparing a compound polysaccharide feed additive for improving the quality of cock semen are performed according to the following steps, all of which are performed in an environment with a temperature of 18-25 ℃ and an air humidity of less than 25%, as follows:
s1, mixing potassium sorbate, tocopherol and tea polyphenol uniformly according to the dosage in the table 1 to prepare a compound polysaccharide stabilizer;
s2, uniformly mixing the agaricus blazei polysaccharide, the lycium barbarum polysaccharide, the seaweed selenium polysaccharide and the compound polysaccharide stabilizer prepared in the step S1 according to the dosage in the table 2 to obtain the compound polysaccharide feed additive for improving the quality of the cock semen.
Examples 7-12 applications of Compound polysaccharide feed additives for improving sperm quality of rooster
Examples 7 to 12 are the applications of the compound polysaccharide feed additive for improving the semen quality of the cocks, and the applications are that the compound polysaccharide feed additive is added into the basic ration of the cocks to feed the cocks, except that the compound polysaccharide feed additive for improving the semen quality of the cocks is added in the amount shown in table 3.
TABLE 3 additive amount of compound polysaccharide feed additive for improving sperm quality of cock
Example 13 verification test of Compound polysaccharide feed additive for improving sperm quality of cock
(1) Test animal selection and grouping
Selecting 40 feathers of healthy Hailan brown parent breeding cocks of 44 weeks old in a certain chicken farm in Tianjin, and dividing the cocks into a control group and a test group after balancing according to the weight, the amount of sperms, the sperm motility and the sperm density; each group had 4 replicates, each replicate 5 feather cocks.
(2) Daily ration and feeding management
Preparing a basic ration according to the NRC and the chicken feeding standard in China by matching the nutrition required by the breeding cocks, feeding the basic ration by a control group, feeding the test ration by adding 0.1 percent of the compound polysaccharide feed additive for improving the sperm quality of the cocks in the example 3 into the basic ration by a test group, and freely feeding and drinking water in the test process and carrying out conventional immunization; after the control group and the test group are both fed with the basic ration for pre-testing for 1 week, the test group starts to feed the test daily ration, the control group starts to continue to feed the basic daily ration, and the test period is 5 weeks, namely 45 weeks to 50 weeks.
(3) Collection and index determination
Semen collection is carried out for 1 week and 5 times when the breeding cock is 50 weeks old, semen collection is carried out for 3 days continuously, 1 time per day, 1 day of rest is carried out, semen collection is carried out for 2 days again, 1 time per day is carried out, and semen collection is carried out manually by adopting an abdominal massage method by a timing member; immediately after artificial semen collection, the amount of semen, sperm motility, sperm density and sperm aberration rate were measured as follows.
Measuring the injection quantity: preheating the semen collection cup in advance, directly reading on the semen collection cup after collecting semen by an abdominal massage method, and determining the semen volume;
measurement of density: dripping semen diluted by 0.3% NaCl solution into a blood cell counting chamber by a dropper (wiping the semen around a cover glass by a piece of lens wiping paper and ensuring that no air bubble exists in the cover glass), then placing the chamber under a high power microscope for observation, recording the number of the sperms in 5 squares in total at 4 corners and the center, wherein the number of the sperms in the grid only exceeds 3/4 during counting, and the counting is carried out when the size of the sperms exceeds 3/4;
the semen density calculation method comprises the following steps:
number of sperm per ml = total number of sperm per 5 squares × 5 × 10 × 1000 × dilution;
measuring the sperm motility: sucking semen diluted by 0.3% NaCl solution by using a dropper, dropping the semen on a glass slide, covering a cover glass, ensuring that no bubbles exist in the semen, observing the semen under a microscope, and calculating the number of the linearly movable sperms in a visual field;
the sperm motility calculation method comprises the following steps:
sperm motility = number of sperm moving straight ahead/total number of sperm in visual field × 100%;
fourthly, measuring the sperm teratogenesis rate: taking semen smears, performing microscopic examination on teratospermia after Giemsa staining, smearing 2 pieces of sperm on each sample, counting 500 sperms on each piece, and calculating the deformity rate;
determination criteria of teratospermia: teratospermia mainly refers to giant head, thin head, defect, double head, pear-shaped head, head protrusion, neck enlargement, mitochondria enlargement, with primary particles, no tail, tail curl and other curls;
the method for calculating the sperm teratogenesis rate comprises the following steps:
sperm aberration (%) = number of teratospermic sperm/500 × 100;
fifthly, measuring the activity of glutathione peroxidase in seminal plasma: the activity of glutathione peroxidase in seminal plasma is measured by a kit provided by Nanjing institute of bioengineering, Inc.
(4) Test results
TABLE 4 index measurement results
Note: indicates that the difference is very significant compared with the control group (P< 0.01 > showing significant difference from control group >P﹤0.05）。
As can be seen from Table 4, the compound polysaccharide feed additive for improving the semen quality of the cocks significantly improves the sperm motility and the sperm density, significantly reduces the sperm aberration rate, significantly improves the glutathione peroxidase activity in seminal plasma, improves the semen quality and the antioxidant level, and is beneficial to prolonging the preservation period of the semen.
Example 14 verification test of Compound polysaccharide feed additive for improving sperm quality of cock
(1) Test animal selection and grouping
Selecting 40 feathers of healthy Hailan brown parent breeding cocks of 41 weeks old in a certain chicken farm in Qingdao, Shandong province, and dividing the cocks into a control group and a test group after balancing according to weight, sperm amount, sperm motility and sperm density; each group had 4 replicates, each replicate 5 feather cocks.
(2) Daily ration and feeding management
Preparing a basic ration according to the NRC and the chicken feeding standard in China by matching the nutrition required by the breeding cocks, feeding the basic ration by a control group, feeding the test ration by adding 0.5 percent of the compound polysaccharide feed additive for improving the sperm quality of the cocks in the example 4 into the basic ration by a test group, and freely feeding and drinking water in the test process and carrying out conventional immunization; after the control group and the test group are both fed with the basic ration for pre-testing for 1 week, the test group starts to feed the test daily ration, the control group starts to continue to feed the basic daily ration, and the test period is 5 weeks, namely 42 weeks to 47 weeks.
(3) Collection and index determination
Semen collection is carried out for 1 week and 5 times when the breeding cock is 47 weeks old, semen collection is continuously carried out for 3 days, 1 time is carried out each day, 1 day is carried out for rest, semen collection is carried out for 2 days again, 1 time is carried out each day, and semen collection is carried out manually by adopting an abdominal massage method by a timing member; the method of index measurement was the same as that of example 7.
(4) Test results
TABLE 5 index measurement results
Note: indicates that the difference is very significant compared with the control group (P< 0.01 > showing significant difference from control group >P﹤0.05）。
As can be seen from Table 5, the compound polysaccharide feed additive for improving the semen quality of the cocks significantly improves the sperm motility and the sperm density, significantly reduces the sperm aberration rate, significantly improves the glutathione peroxidase activity in seminal plasma, improves the semen quality and the antioxidant level, and is beneficial to prolonging the preservation period of the semen.
Claims (6)
1. The compound polysaccharide feed additive for improving the quality of cock semen is characterized in that the raw materials for preparing the effective components comprise 75-90 percent by weight: 8.5-23.5: 1: 0.5 of agaricus blazei murill polysaccharide, lycium barbarum polysaccharide, seaweed selenium polysaccharide and compound polysaccharide stabilizer.
2. The compound polysaccharide feed additive for improving the quality of cock semen as claimed in claim 1, wherein the selenium content in the selenium-rich polysaccharide is 10-20 mg/g.
3. The compound polysaccharide feed additive for improving the quality of the semen of the cock as claimed in claim 1 or 2, wherein the raw materials for preparing the effective components of the compound polysaccharide stabilizer comprise 40% by weight of potassium sorbate, 30% by weight of tocopherol and 30% by weight of tea polyphenol.
4. The preparation method of the compound polysaccharide feed additive for improving the quality of cock semen as claimed in any one of claims 1-3, which comprises the following steps:
s1, mixing potassium sorbate, tocopherol and tea polyphenol uniformly to prepare a compound polysaccharide stabilizer;
s2, uniformly mixing the agaricus blazei polysaccharide, the lycium barbarum polysaccharide, the seaweed selenium polysaccharide and the compound polysaccharide stabilizer prepared in the step S1 to obtain the compound polysaccharide feed additive for improving the quality of the cock semen.
5. The use of the compound polysaccharide feed additive for improving the semen quality of the cocks as claimed in any one of claims 1 to 3, wherein the compound polysaccharide feed additive for improving the semen quality of the cocks is added into basal diet of the cocks to feed the cocks.
6. The application of the compound polysaccharide feed additive as claimed in claim 5, wherein the compound polysaccharide feed additive for improving the quality of the semen of the cocks is added into the basal diet of the cocks according to the addition amount of 0.1-0.5% by weight.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1656931A (en) * | 2005-03-23 | 2005-08-24 | 余姚市泰夫昌农庄有限公司 | Health-care food containing ganoderma polysaccharide and tea polyphenol |
| CN104206815A (en) * | 2014-07-31 | 2014-12-17 | 北京农学院 | Feed for enhancing comprehensive breeding performance of breeder cock |
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- 2020-08-24 CN CN202010857431.5A patent/CN111972565A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1656931A (en) * | 2005-03-23 | 2005-08-24 | 余姚市泰夫昌农庄有限公司 | Health-care food containing ganoderma polysaccharide and tea polyphenol |
| CN104206815A (en) * | 2014-07-31 | 2014-12-17 | 北京农学院 | Feed for enhancing comprehensive breeding performance of breeder cock |
Non-Patent Citations (5)
| Title |
|---|
| 朱宏娟，等: "家禽有机硒营养研究进展", 《中国饲料》 * |
| 汪志君，等: "《食品工艺学》", 30 November 2006, 中国计量出版社 * |
| 罗琼，等: "枸杞多糖与海带多糖联合对慢性腹部电离辐射雄性大鼠生殖损伤的影响", 《营养学报》 * |
| 闫岩: "姬松茸多糖对镉致鸡睾丸毒性损伤的保护作用", 《中国优秀硕士学位论文全文数据库（电子期刊），农业科技辑》 * |
| 陈国良，等: "《功效非凡的食用菌》", 30 June 2005, 上海科学技术出版社 * |
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Application publication date: 20201124 |