CN111962307B - Antibacterial material capable of providing nitric oxide by itself and preparation method and application thereof - Google Patents

Abstract

The invention relates to an antibacterial material capable of self-providing nitric oxide, a preparation method and application thereof, wherein the antibacterial material capable of self-providing nitric oxide comprises a substrate material and a functional material loaded on the substrate material; the functional material includes a NO donor material, a binder, a dispersant, and a water repellent. The NO donor material in the antibacterial material capable of providing nitric oxide can release NO under the stimulation of light, heat or water vapor, so that the antibacterial material has the functions of inhibiting the growth of microorganisms, sterilizing and resisting viruses; the NO donor material is stably adhered to the substrate material by adding the adhesive, the dispersing agent is added to ensure that the NO donor material is uniformly distributed on the substrate material, the waterproof agent helps the slow controlled release behavior of NO, the four materials supplement each other to realize synergistic interaction, meanwhile, the comfort and the air permeability of the antibacterial material can be ensured, and the antibacterial material is particularly suitable for being used as a preparation raw material of sanitary towels or protection pad products, and the duration time of NO release is kept to be about 2-5 hours under the triggering of water vapor.

Description

An antibacterial material capable of self-supplying nitric oxide, its preparation method and application

technical field

The invention belongs to the technical field of antibacterial materials, in particular to an antibacterial material and its preparation method and application, especially to an antibacterial material capable of self-providing nitric oxide, its preparation method and its application in the preparation of sanitary napkins or protective pads .

Background technique

Sanitary napkin or panty liner is an absorbent material, the main material is cotton, non-woven fabric, pulp or polymer composite paper formed by a composite of the above materials, used to absorb women's menstrual cramps menstrual blood from the vagina. Because women will discharge a large amount of menstrual blood during menstruation, a small local moist environment will be formed between the sanitary napkin and the vagina, which is very suitable for the growth of bacteria. At the same time, combined with the composition characteristics of menstrual blood, it can provide nutrients for the growth of bacteria, which is more conducive to the reproduction of bacteria. Moreover, women’s resistance decreases during menstruation. If a large number of bacteria gather near the vagina at this time, bacterial infection is easy to occur, which in turn leads to a series of gynecological diseases, which are reflected in clinical manifestations, usually genital itching, leucorrhea Increase and backache and other symptoms. However, some sanitary napkins with antibacterial effect in the prior art have the disadvantages that they are not highly targeted and cannot effectively play an antibacterial effect.

CN102961773A discloses a preparation method of chitin antibacterial sanitary napkin, preparing chitin antibacterial fiber, the mass ratio is (20%-40%): (80%-60%) chitin antibacterial fiber and ES fiber are passed through an opener Opening and mixing, setting according to the weight requirements, setting by hot air, net laying, winding, and cutting, to make chitin biological antibacterial non-woven fabrics; when making sanitary napkins, after making core materials, spray glue to cover the carapace Su biological antibacterial non-woven material; then, groove forming, cover the bottom film, paste the back wing release paper, seal the shape, cut and form, cover the film and quick and easy paste, horizontally seal, cut, and make the carapace Antibacterial Sanitary Napkins.

CN204798138U discloses a sanitary napkin provided with mineral-plant compound health-care antibacterial chip layer, including a skirt and a sanitary napkin body, and the sanitary napkin body includes a superfine fiber non-woven fabric, a fiber deflector, and a 360-degree coated non-woven fabric. Dust paper, kapok layer and mineral-plant compound health-care antibacterial chip; the sanitary napkin with mineral-plant compound health-care antibacterial chip layer is equipped with mineral-plant compound health-care antibacterial chip inside, which can help neutralize oxidative substances and relieve menstrual discomfort. Oxygen and nutrients are fully absorbed into the cells.

Nitric oxide widely exists in various tissues and cells of the human body and has important physiological functions. Nitric oxide, also known as "vascular endothelial relaxation factor", can relax vascular smooth muscle and inhibit platelet aggregation. It can also act on adjacent peripheral neurons such as presynaptic nerve endings and astrocytes through diffusion, and reactivate Guanylate cyclase thus increases the level of cyclic guanosine monophosphate to produce physiological effects, such as inducing long-term potentiation effects related to learning and memory. Nitric oxide is also an effective broad-spectrum antibacterial and fungal agent. Normal nasal mucosal epithelial cells can produce high concentrations of nitric oxide to prevent respiratory tract infections. Nitric oxide produced by macrophages can kill a variety of pathogens, including Escherichia coli and Staphylococcus aureus associated with common medical device infections. Studies have shown that nitric oxide at the nM level is sufficient to effectively destroy biofilms formed by various bacterial strains. Polymer materials containing nitric oxide donors can also effectively control bacterial infections. Nitric oxide is a free radical gas molecule with a very short half-life, and it is not easy to produce drug resistance and toxic side effects during the action process. The properties of nitrogen itself gas and short half-life are not conducive to storage and practical use, which greatly limits the application of nitric oxide as a bacteriostat or antibacterial agent for sanitary products.

Contents of the invention

Aiming at the deficiencies in the prior art, the object of the present invention is to provide a kind of antibacterial material and its preparation method and application, especially to provide a kind of antibacterial material which can self-provide nitric oxide and its preparation method and its use in the preparation of sanitary napkin or panty liner in the application.

To achieve this purpose of the invention, the present invention adopts the following technical solutions:

In a first aspect, the present invention provides an antibacterial material that can self-provide nitric oxide, the antibacterial material that can self-provide nitric oxide includes a base material and a functional material loaded on the base material; the functional material includes NO Donor materials, binders, dispersants and water repellents.

The NO donor material in the antibacterial material that can self-provide nitric oxide involved in the present invention can release NO under the stimulation of light, heat or water vapor, and play the role of inhibiting microbial growth, sterilization and anti-virus; but the simple NO donor The material is unevenly distributed on the base material and is easy to fall off, which ultimately affects the antibacterial performance of the material. The present invention makes the NO donor material adhere to the base material stably by adding an adhesive, and adds a dispersant to ensure that the NO donor material Evenly distributed on the base material, the water repellent helps the slow and controlled release of NO, the four complement each other and synergize, and at the same time can ensure the comfort and air permeability of the antibacterial material, especially suitable for the preparation of sanitary napkins or panty liners , under the trigger of water vapor, the duration of NO release is kept at about 2-5h.

Preferably, the functional material includes 4-7 parts by mass of NO donor material, 1-5 parts of binder, 0.1-10 parts of dispersant and 1-30 parts of waterproofing agent.

The four kinds of functional materials in the antibacterial material that can self-provide nitric oxide involved in the present invention can make the adhesion, stability, dispersion uniformity, and NO donor material utilization rate of the material under the proportioning ratio of the above-mentioned parts by mass. , antibacterial to achieve better results. If the number of adhesives relative to the NO donor material is too large, the hardness of the non-woven fabric will increase, and the structural pores between the non-woven fabrics will be blocked, resulting in a decrease in the local air permeability of the sanitary napkin pad. Reduce the total amount of donors adhering to the base material, resulting in a decrease in the utilization of NO donors; too many parts of the dispersant relative to the NO donor material will lead to a decrease in adhesive adhesion and concentration decay, thereby weakening The role of its adhesion, if the number of parts is too small, the donor will not be completely evenly distributed in the solution, and the dispersion will be unstable and easy to form donor sedimentation, resulting in the appearance of particles when sprayed on the non-woven fabric. The distribution on the surface is uneven; the number of parts of the waterproofing agent relative to the NO donor material is too large, and the donor will not be completely released during the process of using sanitary napkins by the human body until the replacement time, and the number of parts is too small. In , the donor releases NO time too fast to meet the required wearing time.

The mass parts of the NO donor material can be 4 parts, 5 parts, 6 parts or 7 parts, etc. Any specific point value within the above numerical range can be selected, and details will not be repeated here.

The mass parts of the waterproofing agent can be 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 12 parts, 13 parts, 15 parts, 18 parts, 20 parts, 25 parts or 30 parts, etc., the above numerical range Any specific point value in can be selected, and will not be repeated here.

The mass parts of the adhesive can be 1 part, 2 parts, 3 parts, 4 parts or 5 parts, etc. Any specific point value within the above numerical range can be selected, and details will not be repeated here.

The number of parts by mass of the dispersant can be 0.1 part, 0.2 part, 0.6 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 8 parts or 10 parts, etc. The specific point values can be selected, and will not be repeated here.

Preferably, the loading rate of the NO donor material on the base material is 50%-90%.

In the antibacterial chip involved in the present invention, the loading rate of the NO donor material on the base material is specifically selected as 50%-90%, because if the loading rate is too high, the finished non-woven fabric will have high hardness and decreased air permeability. , If the load rate is too low, the donor utilization rate will decrease and the cost will increase.

Preferably, the base material includes non-woven fabric, melt-blown fabric, filter cotton, polypropylene non-woven fabric or sponge, preferably non-woven fabric.

The base material that the present invention relates to can self-provide the antibacterial material of nitric oxide can choose above-mentioned type, single factor compares the property of above-mentioned each base material and finds, the water permeability and water vapor permeability of filter cotton, polypropylene non-woven fabric and sponge are all Better, the dispersion uniformity of functional materials on it is poor; the water permeability and water vapor permeability of melt blown cloth are poor, and the dispersion uniformity of functional materials on it is also poor; the water permeability of non-woven fabrics is poor, but the water vapor permeability The performance is good, and the dispersion uniformity of functional materials on it is also good, so non-woven fabric is more preferred as the base material.

Preferably, the NO donor material includes nitrosoferric sodium hydride, S-nitroso-N-acetylpenicillamine, S-nitrosoglutathione, azodiol series compounds or even Any one or a combination of at least two of the carbazole-loaded polymers, such as S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine The combination of sodium nitrosoferric hydride and azocenediol series compounds, etc., any other combination can be selected, and will not be repeated here. S-nitrosoglutathione is preferred.

In the antibacterial material that can provide nitric oxide involved in the present invention, the NO donor material can be selected from the above-mentioned types, wherein preferred S-nitrosoglutathione (GSNO) or S-nitroso-N-acetylcyanine Mycamine (SNAP) two materials are used as NO donor materials, S-nitrosoglutathione powder is light pink, and S-nitroso-N-acetylpenicillamine powder is light green, making the antibacterial functional material overall It is light pink or light green. In actual use, with the release of NO, the material gradually changes from light pink or light green to colorless. The longer the time, the lighter the color, which serves as an indicator for the user. The side reflects the release rate of NO and the antibacterial activity of the material. If it is used to prepare sanitary napkins or panty liners, during use, due to the effect of water vapor, it can stimulate the release of NO, thereby inhibiting microorganisms in the local environment, and with the prolongation of use time, the color of sanitary napkins or panty liner pads will change. It gradually changes from light pink or light green to colorless, which reminds users of "the remaining amount of antibacterial activity" and timely replacement of sanitary napkins or panty liners.

While comparing the two materials of S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine, the former is an endogenous substance without odor, while the latter is not an endogenous substance, and There is a certain smell, and subsequent use needs to take other methods to remove or cover up the smell. Therefore, S-nitrosoglutathione is more preferred as the NO donor material in the antibacterial material capable of self-supplying nitric oxide of the present invention.

Preferably, the binder includes any one or a combination of at least two of dextrin, hydroxymethyl cellulose, silica gel, pectin or polyvinyl acetate latex, the combination of at least two such as dextrin and The combination of hydroxymethyl cellulose, the combination of silica gel and pectin, the combination of pectin and polyvinyl acetate latex, etc., any other combination can be selected, and will not be repeated here. Polyvinyl acetate latex is preferred.

Compared to other adhesive types, polyvinyl acetate latex is superior in its ability to promote the adhesion of functional materials to substrate materials.

Preferably, the dispersant includes any one or a combination of at least two of polyvinyl alcohol, polyethylene glycol, sodium lauryl sulfate or sodium dodecyl sulfate, and the combination of at least two For example, the combination of polyvinyl alcohol and polyethylene glycol, the combination of sodium lauryl sulfate and sodium lauryl sulfate, the combination of polyvinyl alcohol and sodium lauryl sulfate, etc., any other combination can be selected, I won’t go into details here. A combination of polyvinyl alcohol and sodium lauryl sulfate is preferred.

Compared with other dispersant types, the combination of polyvinyl alcohol and sodium lauryl sulfate is more significant in promoting the uniformity of dispersion of functional materials on substrate materials.

Preferably, the waterproofing agent includes any one or a combination of at least two of simethicone, liquid paraffin, solid paraffin, silicone resin, lanolin, natural resin or petrolatum; The combination of paraffin wax and solid paraffin, the combination of simethicone and silicone resin, the combination of silicone resin and lanolin, etc., any other combination can be selected, and will not be repeated here. Natural resins are preferred.

Compared with other types of waterproofing agents, natural resins have a better appearance, no stickiness, and no falling off when used in the scene of antibacterial chips in sanitary napkin pads. At the same time, they can make the antibacterial chips donate nitric oxide after passing through the water system It releases NO slowly and has a better effect in waterproofing.

In a second aspect, the present invention provides a method for preparing an antibacterial material that can self-provide nitric oxide as described above, the preparation method comprising:

(1) mixing NO donor material, binder, dispersant and solvent to prepare coating liquid;

(2) Spray the coating solution prepared in step (1) onto the base material, dry, and finally apply a waterproofing agent on the surface of the base material to obtain the antibacterial material that can provide nitric oxide by itself.

The spray needs to be sprayed slowly at a uniform speed, and the speed should not be too high, so as to prevent the water molecules of the solution from penetrating through the pores of the base material and fail to achieve the retention effect. The preparation method of the antibacterial material capable of self-providing nitric oxide involved in the present invention has simple process, easy operation and is suitable for industrial production.

Preferably, step (1) specifically includes the following steps:

(a) mixing the dispersant with the solvent and stirring to prepare solution A; mixing the NO donor material with the solvent and stirring, then mixing with the binder and stirring to obtain solution B;

(b) Mix and stir the solution A and solution B prepared in step (a) to prepare a coating liquid.

The preparation of solution B in step (a) will affect the visual effect of the antibacterial material after coating. If the donor material is not fully dissolved in the solvent, pink plaques of different sizes will be formed after spraying, which will affect the overall aesthetic feeling of the material.

When the antibacterial material is prepared in the present invention, the above specific preparation sequence of the coating solution not only ensures the release of the NO donor material, but also facilitates the adhesion of the donor material and the base material, and finally exerts the self-cleaning performance of inhibiting microorganisms.

The purpose of the drying is to allow the NO donors to better adhere to the base material. You can choose a clean and flat platform, volatilize and dry within a specified time, and the donor material penetrates into the base material. At this time, the NO donors in the solution The adhesive acts as an adhesive to better lock the donor to the base material. It cannot be exposed to sunlight or dried under hot and strong wind. Sunlight and hot wind will cause the donor to release NO early, resulting in a decrease in the effective content of the donor.

Preferably, the drying temperature in step (2) is 20-40°C, such as 20°C, 25°C, 30°C, 35°C or 40°C, etc. Other specific point values in the range can be selected, and it will not be mentioned here Let me repeat them one by one.

Preferably, the drying time is 1-3h, such as 1h, 1.5h, 2h, 2.5h or 3h, etc. Other specific points within the range can be selected, and will not be repeated here.

Preferably, the operation of spraying the coating liquid onto the base material in step (2) is performed 1-5 times in total, such as 1 time, 2 times, 3 times, 4 times or 5 times.

In a third aspect, the present invention provides an application of the above-mentioned self-providing nitric oxide antibacterial material in the preparation of sanitary napkins or panty liners.

Compared with the prior art, the present invention has the following beneficial effects:

The NO donor material in the antibacterial material that can self-provide nitric oxide involved in the present invention can release NO under the stimulation of light, heat or water vapor, and play the role of inhibiting microbial growth, sterilization and anti-virus; but the simple NO donor The material is unevenly distributed on the base material and is easy to fall off, which ultimately affects the antibacterial performance of the material. The present invention makes the NO donor material adhere to the base material stably by adding an adhesive, and adds a dispersant to ensure that the NO donor material Evenly distributed on the base material, the water repellent helps the slow and controlled release of NO, the four complement each other and synergize, and at the same time can ensure the comfort and air permeability of the antibacterial material, especially suitable for the preparation of sanitary napkins or panty liners , under the trigger of water vapor, the duration of NO release is kept at about 2-5h.

When GSNO or SNAP is used as NO donor, if the antibacterial material that can self-provide nitric oxide is used to prepare sanitary napkins or panty liners, the color will change from pale pink or The light green gradually turns colorless, which reminds the user of the "remaining amount of antibacterial activity" and timely replacement of sanitary napkins or panty liners.

Description of drawings

Fig. 1 is the color change diagram of the product prepared in Example 1 before and after releasing NO;

Fig. 2 is the plate colony diagram when the product of embodiment 1 is carrying out antibacterial investigation;

Fig. 3 is the plate colony figure of kanamycin group when carrying out antibacterial investigation;

Fig. 4 is the plate colony diagram when silver ion group is carrying out antibacterial investigation;

Figure 5 is a plate colony diagram of the blank control group when the antibacterial property was investigated.

Detailed ways

The technical solutions of the present invention will be further described below through specific embodiments. It should be clear to those skilled in the art that the embodiments are only for helping to understand the present invention, and should not be regarded as specific limitations on the present invention.

Example 1

This embodiment prepares a kind of antibacterial material that can self-provide nitric oxide, comprises base material (non-woven fabric) and the functional material loaded on the described non-woven fabric; Said functional material comprises NO donor material (GSNO), Binders (polyvinyl acetate latex), dispersants (polyvinyl alcohol and sodium lauryl sulfate) and water repellents (natural resins). Its preparation method is as follows:

(1) Mix 20mg of polyvinyl alcohol, 5mg of sodium lauryl sulfate with 10mL of water, and stir at 23°C for 10min to prepare solution A; mix 500mg of GSNO with 10mL of water, stir at 23°C for 5min, then mix with 250mg of polystyrene Mix vinyl acetate latex and stir at 23°C for 5 minutes to prepare solution B;

(2) Mix solution A and solution B prepared in step (1), and stir at 23° C. for 5 minutes to obtain a coating solution;

(3) Evenly spray the coating solution obtained in step (2) onto a nonwoven fabric with a thickness of 0.185 mm, volatilize and dry, and repeat the spraying again. Finally, the loading rate of GSNO on the nonwoven fabric is 83%. volatilize and dry again;

(4) Place 800mg of waterproofing agent (natural resin) on both sides of the roller and evenly coat the front and back of the product surface in step (3) to obtain the antibacterial material that can provide nitric oxide by itself.

Example 2

This embodiment prepares a kind of antibacterial material that can self-provide nitric oxide, comprises base material (non-woven fabric) and the functional material loaded on the described non-woven fabric; Said functional material comprises NO donor material (SNAP), Binders (polyvinyl acetate latex), dispersants (polyvinyl alcohol and sodium lauryl sulfate) and water repellents (natural resins). Its preparation method is as follows:

(1) Mix 10mg of polyvinyl alcohol, 2.5mg of sodium lauryl sulfate with 5mL of water, and stir at 23°C for 10min to prepare solution A; mix 250mg of SNAP with 2mL of water, stir at 23°C for 5min, then mix with 125mg Mix polyvinyl acetate latex, stir at 23°C for 5 minutes, and finally add SNAP solubilizer 3mL absolute ethanol and stir at 23°C for 5 minutes to prepare solution B;

(2) Mix solution A and solution B prepared in step (1), and stir at 23° C. for 5 minutes to obtain a coating solution;

(3) Evenly spray the coating solution obtained in step (2) onto a 0.185mm non-woven fabric, volatilize and dry, and repeat the spraying again. Finally, the loading rate of SNAP on the non-woven fabric is 84%, and volatilizes again dry;

(4) Put 500mg of waterproofing agent (natural resin) on both sides of the roller and apply it on the front and back of the product in step (3), to obtain the antibacterial material that can provide nitric oxide by itself.

Example 3

This embodiment prepares a kind of antimicrobial material that can self-provide nitric oxide, and the difference of its structural composition and embodiment 1 is only that dispersant (polyvinyl alcohol and sodium lauryl sulfate) is replaced with the dispersant of equal weight (ten Dialkyl sodium sulfate), in the preparation method step (1): 25mg sodium lauryl sulfate is mixed with 10mL water. All other conditions remain unchanged.

Example 4

This example prepares an antibacterial material that can self-provide nitric oxide. The difference between its structure and composition and Example 1 is that the adhesive polyvinyl acetate latex is replaced by an equal amount of adhesive silica gel, and other conditions are kept the same. . Its preparation method refers to Example 1.

Example 5

In this example, an antibacterial material capable of self-supplying nitric oxide is prepared, and its structure and composition are consistent with Example 1. Its preparation method is slightly different from Example 1:

(1) Mix 20mg of polyvinyl alcohol, 5mg of sodium lauryl sulfate, 500mg of GSNO, 250mg of polyvinyl acetate latex with 20mL of water, and stir at 23°C for 10min to prepare a coating solution;

(2) Evenly spray the coating solution obtained in step (1) onto a non-woven fabric with a thickness of 0.185mm, volatilize and dry, and repeat the spraying again. Finally, the loading rate of GSNO on the non-woven fabric is 80%. volatilize and dry again;

(3) Place 800mg of waterproofing agent (natural resin) on both sides of the roller and apply it to the front and back of the product in step (2) to obtain the antibacterial chip.

Comparative example 1

In this comparative example, an antibacterial material capable of self-supplying nitric oxide was prepared. The difference between its structure and composition and that of Example 1 is that it does not contain a binder component, and other conditions remain the same. Its preparation method is as follows:

(1) Mix 20mg of polyvinyl alcohol, 5mg of sodium lauryl sulfate with 10mL of water, and stir at 23°C for 10min to prepare solution A; mix 500mg of GSNO with 10mL of water, and stir at 23°C for 5min to prepare solution B ;

(2) Mix solution A and solution B prepared in step (1), and stir at 23° C. for 5 minutes to obtain a coating solution;

(3) Evenly spray the coating solution obtained in step (2) onto a nonwoven fabric with a thickness of 0.185mm, volatilize and dry, and repeat the spraying again. Finally, the loading rate of GSNO on the nonwoven fabric is 47%. volatilize and dry again;

(4) Place 800mg of water repellent (natural resin) on both sides of the roller and apply it on the surface of the product in step (3) to obtain the antibacterial material that can self-provide nitric oxide.

Comparative example 2

In this comparative example, an antibacterial material capable of self-supplying nitric oxide was prepared. The difference between its structure and composition and that of Example 1 is that it does not contain a dispersant component, and other conditions remain the same. Its preparation method is as follows:

(1) Mix 500mg GSNO with 10mL water, stir at 23°C for 5min, then mix with 250mg of polyvinyl acetate latex, stir at 23°C for 5min to prepare solution B;

(2) Mix solution A and solution B prepared in step (1), and stir at 23° C. for 5 minutes to obtain a coating solution;

(3) Evenly spray the coating solution obtained in step (2) onto a nonwoven fabric with a thickness of 0.185 mm, volatilize and dry, and repeat the spraying again. Finally, the loading rate of GSNO on the nonwoven fabric is 70%. volatilize and dry again;

(4) Place 800mg of water repellent (natural resin) on both sides of the roller and apply it on the surface of the product in step (3) to obtain the antibacterial material that can self-provide nitric oxide.

Comparative example 3

In this comparative example, an antibacterial material capable of self-supplying nitric oxide was prepared. The difference between its structure and composition and that of Example 1 is that it does not contain a water repellent component, and other conditions remain the same. Its preparation method is as follows:

(1) Mix 20mg of polyvinyl alcohol, 5mg of sodium lauryl sulfate with 10mL of water, and stir at 23°C for 10min to prepare solution A; mix 500mg of GSNO with 10mL of water, stir at 23°C for 5min, then mix with 250mg of polystyrene Mix vinyl acetate latex and stir at 23°C for 5 minutes to prepare solution B;

(2) Mix solution A and solution B prepared in step (1), and stir at 23° C. for 5 minutes to obtain a coating solution;

(3) Evenly spray the coating solution obtained in step (2) onto a nonwoven fabric with a thickness of 0.185 mm, volatilize and dry, and repeat the spraying again. Finally, the loading rate of GSNO on the nonwoven fabric is 83%. Volatilize and dry again; obtain the antibacterial material that can self-provide nitric oxide.

Evaluation test:

(1) Adhesion evaluation:

The adhesiveness of the products prepared in Examples 1-5 and Comparative Examples 1-3 was inspected, and the evaluation method was as follows: based on the prepared samples, after rubbing for different times, the weight changes before and after the samples were compared to evaluate Adhesion, if there is no significant change in weight before and after rubbing, it indicates good adhesion, and if the weight changes significantly before and after rubbing, it indicates poor adhesion. The results are shown in Table 1.

Table 1

group Untreated (mg) Knead for 1min(mg) Knead for 2min(mg) Example 1 80.82 80.40 80.21 Example 2 81.16 80.65 80.32 Example 3 79.49 78.57 78.02 Example 4 79.17 78.58 77.49 Example 5 79.89 79.53 79.20 Comparative example 1 68.84 65.97 63.95 Comparative example 2 76.50 75.45 74.38 Comparative example 3 80.82 80.40 79.86

From the data in Table 1, it can be seen that compared with Comparative Example 1, the weight of the antibacterial chip in Example 1 and Example 2 did not change significantly after rubbing, indicating that the NO donor can better adhere to the base material under the action of the adhesive. superior.

(2) Stability evaluation:

The stability of the products prepared in Examples 1-5 and Comparative Examples 1-3 is investigated. The evaluation method is as follows: this experiment is an accelerated experiment, and the samples are placed in a 4°C refrigerator and a 45°C incubator respectively. Weigh (in mg) and take pictures, the results are shown in Table 2 (4°C) and Table 3 (45°C).

Table 2

table 3

group 0 weeks (mg) 4 weeks (mg) 9 weeks (mg) 18 weeks (mg) Example 1 80.89 80.85 80.83 80.77 Example 2 81.02 81.00 80.98 80.95 Example 3 79.46 79.44 79.41 79.34 Example 4 79.17 79.15 79.13 79.10 Example 5 79.90 79.85 79.60 79.40 Comparative example 1 68.92 68.90 68.87 68.78 Comparative example 2 76.41 76.39 76.35 76.29 Comparative example 3 80.79 80.68 80.54 80.20

From the data in Table 2 and Table 3, it can be known that the stability acceleration test results of each embodiment and comparative example show that under two different temperature environments, the quality of the front and back of the pad chip does not change significantly, and the color does not change at the same time, indicating that The nitric oxide donor coated on the surface of the pad chip has good stability and can be stored stably for a long time.

(3) Evaluation of dispersion uniformity:

The dispersion uniformity of the products prepared in Examples 1-5 and Comparative Examples 1-3 was inspected, and the evaluation method was specifically: since there are two types of NO donors, pink and light green, can the donors be evenly distributed in the NO donor? On woven fabrics, it can be roughly evaluated by human visual senses. After spraying, the non-woven fabric with the same volume and size has no plaque, the overall color is relatively uniform, and there is no aggregation of donor particles at the same time, it is rated as good dispersion uniformity, and the results are shown in Table 4.

Table 4

group With or without plaque Example 1 no plaque Example 2 no plaque Example 3 small amount of plaque Example 4 small amount of plaque Example 5 small amount of plaque Comparative example 1 no plaque Comparative example 2 Many plaques Comparative example 3 no plaque

From the data in Table 4, it can be seen that the formation of plaques is due to the uneven dispersion of the donor particles, and the small lumps of substances gathered together. The actual sensory comparison statistics show that the uniformity of Examples 1 and 2 is better, and there is no plaque, while Comparative Example 2 had many plaques.

(4) Evaluation of NO donor utilization rate:

The products obtained in Examples 1-5 and Comparative Examples 1-3 were investigated on the utilization rate of NO donors, and the utilization rate of NO donors was the ratio of the actual donor load of the final product to the amount of donor feed. The results are shown in Table 5 shown.

table 5

From the data in Table 5, it can be seen that compared with Comparative Example 1, the donor utilization rate of Example 1 and Example 2 is higher, indicating that the presence of the binder can effectively improve the utilization rate of the NO donor, and increase the donor on the substrate. The amount of attachment reduces donor waste.

(5) The products prepared in Examples 1-5 and Comparative Examples 1-3 were investigated in an environment with water vapor, and the specific method was: the samples were exposed to the aqueous solution under a nitrogen environment, and the effect was different for different times. : 1h, 2h, 3h, 4h; take the solutions of the above different time periods, use the nitric oxide detection kit to detect the release concentration of NO at different times, and record the results of the NO concentration (μmol) of each group changing with time (h) , and the results are shown in Table 6.

Table 6

group 1h 2 hours 3 hours 4 hours Example 1 18.62 38.60 55.72 57.54 Example 2 18.62 39.24 57.47 58.14 Example 3 17.92 35.12 52.08 54.63 Example 4 17.50 36.26 51.38 53.91 Example 5 18.49 38.10 53.86 55.46 Comparative example 1 17.22 25.90 29.96 32.48 Comparative example 2 16.13 30.94 44.80 48.44 Comparative example 3 45.29 57.45 57.13 57.43

It can be seen from the data in Table 6 that: under the condition of simulated water vapor, Example 1 and Example 2 are completely released in about 3 hours, and under the normal use time of the pad chip, the NO is completely released to achieve long-term antibacterial effect; while the product of Comparative Example 3 The release of NO was too fast, reaching a plateau in less than 2 hours, and the long-term antibacterial effect could not be achieved.

The product prepared in Example 1 was photographed and observed before the test and after 3 hours of the test, and the results are shown in Figure 1, wherein the left side is the product picture before illumination, the non-woven fabric is red, and the right side is the product picture after illumination , the red color disappears.

(6) Antibacterial evaluation

The antibacterial properties of the products prepared in Examples 1-5 and Comparative Examples 1-3 were investigated. The evaluation method was as follows: In this experiment, by simulating the application of antibacterial chips on sanitary napkins, NO donors continuously and slowly release monoxide in water. Nitrogen, the duration is 3 hours, and the process of release is also a process of color change, from pink to colorless gradually, the color can be used as a measure of judging the content of polymer material donors and the appearance of NO release. Therefore, we took different groups of non-woven fabrics with a diameter of 6mm and soaked them in the same amount of bacterial solution for 3 hours, and then took a certain amount of soaking solution to culture and count the colonies, and judge the antibacterial effect of different groups by the number of colonies. (With kanamycin and silver ions as the positive control group, the concentration of 1 mg/mL kanamycin solution is sprayed on the non-woven fabric and air-dried to obtain it; the silver ion non-woven fabric is purchased; a blank control group is set at the same time) , the results are shown in Table 7 (each group was measured in parallel three times, and the data in the table are the average value of three determinations).

Table 7

The sterilizing conditions of Example 1 group, Kanamycin group, Silver ion group, and blank control group are shown in Figures 2, 3, 4, and 5 respectively, as can be seen from the figure: Example 1 group can effectively kill grapes aureus cocci, the antibacterial rate was as high as 98.2%, which was slightly lower than that of the kanamycin group. It is worth mentioning that the silver ion pads used in the market have almost no antibacterial activity.

The applicant declares that the present invention illustrates a self-providing nitric oxide antibacterial material and its preparation method and application through the above examples, but the present invention is not limited to the above examples, that is, it does not mean that the present invention Must rely on the above-mentioned embodiment to implement. Those skilled in the art should understand that any improvement of the present invention, the equivalent replacement of each raw material of the product of the present invention, the addition of auxiliary components, the selection of specific methods, etc., all fall within the scope of protection and disclosure of the present invention.

The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention. These simple modifications All belong to the protection scope of the present invention.

In addition, it should be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable way if there is no contradiction. The combination method will not be described separately.

Claims (12)

1. An antibacterial material capable of self-supplying nitric oxide, which is characterized by comprising a base material and a functional material loaded on the base material; the functional material comprises, by mass, 4-7 parts of NO donor material, 1-5 parts of adhesive, 0.1-10 parts of dispersant and 1-30 parts of waterproofing agent;

the NO donor material comprises S-nitroso-N-acetylpenicillamine and/or S-nitrosoglutathione;

the waterproof agent is natural resin;

the dispersing agent is a combination of polyvinyl alcohol and sodium dodecyl sulfate.

2. The self-nitric oxide-supplying antimicrobial material according to claim 1, wherein said base material comprises a non-woven fabric, a filter cotton or a sponge.

3. The self-nitric oxide donating antimicrobial material of claim 1, wherein said base material is a nonwoven fabric.

4. The self-nitric oxide donating antimicrobial material of claim 1, wherein said NO donor material is S-nitrosoglutathione.

5. The self-nitric oxide donating antimicrobial material of claim 1, wherein said binding agent comprises any one or a combination of at least two of dextrin, hydroxymethyl cellulose, silica gel, pectin, or polyvinyl acetate latex.

6. The self-nitric oxide donating antimicrobial material of claim 1, wherein said adhesive comprises polyvinyl acetate latex.

7. The method of preparing the nitric oxide self-supplying antibacterial material according to any one of claims 1 to 6, comprising:

(1) Mixing NO donor material, adhesive, dispersant and solvent to prepare coating liquid;

(2) And (2) spraying the coating liquid prepared in the step (1) onto a substrate material, drying, and finally coating a waterproof agent on the surface of the substrate material to obtain the antibacterial material capable of self-providing nitric oxide.

8. The method for preparing the antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the step (1) comprises the following steps:

(a) Mixing a dispersant and a solvent, and stirring to prepare a solution A; mixing the NO donor material with a solvent, stirring, mixing with an adhesive, and stirring to obtain a solution B;

(b) And (B) mixing the solution A and the solution B prepared in the step (a), and stirring to prepare a coating liquid.

9. The method for preparing an antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the drying temperature in the step (2) is 20 to 40 ℃.

10. The method for preparing an antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the drying time is 1 to 3 hours.

11. The method for preparing an antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the spraying of the coating solution onto the base material in the step (2) is performed 1 to 5 times in total.

12. Use of the self-nitric oxide donating antibacterial material of any one of claims 1-6 in the manufacture of a sanitary napkin or panty liner.

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