CN111962307B - Antibacterial material capable of providing nitric oxide by itself and preparation method and application thereof - Google Patents
Antibacterial material capable of providing nitric oxide by itself and preparation method and application thereof Download PDFInfo
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- CN111962307B CN111962307B CN202010784891.XA CN202010784891A CN111962307B CN 111962307 B CN111962307 B CN 111962307B CN 202010784891 A CN202010784891 A CN 202010784891A CN 111962307 B CN111962307 B CN 111962307B
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Abstract
The invention relates to an antibacterial material capable of self-providing nitric oxide, a preparation method and application thereof, wherein the antibacterial material capable of self-providing nitric oxide comprises a substrate material and a functional material loaded on the substrate material; the functional material includes a NO donor material, a binder, a dispersant, and a water repellent. The NO donor material in the antibacterial material capable of providing nitric oxide can release NO under the stimulation of light, heat or water vapor, so that the antibacterial material has the functions of inhibiting the growth of microorganisms, sterilizing and resisting viruses; the NO donor material is stably adhered to the substrate material by adding the adhesive, the dispersing agent is added to ensure that the NO donor material is uniformly distributed on the substrate material, the waterproof agent helps the slow controlled release behavior of NO, the four materials supplement each other to realize synergistic interaction, meanwhile, the comfort and the air permeability of the antibacterial material can be ensured, and the antibacterial material is particularly suitable for being used as a preparation raw material of sanitary towels or protection pad products, and the duration time of NO release is kept to be about 2-5 hours under the triggering of water vapor.
Description
Technical Field
The invention belongs to the technical field of antibacterial materials, particularly relates to an antibacterial material, a preparation method and an application thereof, and particularly relates to an antibacterial material capable of providing nitric oxide by itself, a preparation method thereof and an application thereof in preparing sanitary towels or protection pads.
Background
A sanitary towel or pad is an absorbent material, mainly made of cotton, non-woven fabric, paper pulp or high molecular polymer and high molecular polymer composite paper, and is used for absorbing menstrual blood flowing out from vagina when women have come to a tide. Because women can discharge a large amount of menstrual blood during the menstrual period, a local moist microenvironment is formed between the sanitary towel and the vagina, and the sanitary towel is very suitable for the breeding of bacteria. Meanwhile, the components of menstrual blood are matched for forming characteristics, so that nutritional ingredients can be provided for breeding of bacteria, and the propagation of the bacteria is facilitated. Moreover, the female resistance is reduced during the menstrual period, and if a large amount of bacteria are accumulated near the vagina at the moment, bacterial infection is easy to occur, so that a series of gynecological diseases are caused, and the symptoms such as pudendum pruritus, leukorrhagia, waist soreness and the like usually appear in clinical manifestations. However, some sanitary napkins with antibacterial effect in the prior art have the disadvantages of weak pertinence and incapability of effectively playing the antibacterial effect.
CN102961773A discloses a preparation method of chitin antibacterial sanitary napkin, which comprises preparing chitin antibacterial fiber, opening and mixing the chitin antibacterial fiber and ES fiber in a mass ratio of (20% -40%) (80% -60%) by an opener, setting according to gram weight, hot air shaping, lapping, rolling and cutting to obtain chitin biological antibacterial non-woven fabric; when the sanitary towel is manufactured, after a core material is manufactured, chitin biological antibacterial non-woven fabric material is sprayed and coated with glue; then, forming a concave channel, covering a bottom film, sticking back wing release paper, sealing and shaping the periphery, forming the external tangent, covering a film, quickly and easily sticking, sealing transversely and cutting to prepare the chitin biological antibacterial sanitary towel.
CN204798138U discloses a sanitary towel with a mineral plant compound health-care antibacterial chip layer, which comprises a protective enclosure and a sanitary towel body, wherein the sanitary towel body comprises superfine fiber non-woven fabric, a fiber flow guide sheet, 360-degree coated dust-free paper, a wood cotton layer and a mineral plant compound health-care antibacterial chip; the sanitary towel with the mineral plant compound health-care antibacterial chip layer is internally provided with the mineral plant compound health-care antibacterial chip, and can help to neutralize oxidizing substances, relieve discomfort in menstrual period and fully absorb oxygen and nutrition into cells.
Nitric oxide is widely present in various tissues and cells of the human body and has important physiological functions. Nitric oxide, also known as vasodilator of the vascular endothelium, relaxes vascular smooth muscle and inhibits platelet aggregation, and also acts on adjacent peripheral neurons such as presynaptic nerve endings and astrocytes by diffusion, reactivating guanylate cyclase and increasing the level of cyclic guanosine monophosphate, thus producing physiological effects such as induction of long-term potentiation associated with learning and memory. Nitric oxide is also an effective broad-spectrum antibacterial and antifungal agent, and normal nasal mucosal epithelial cells are capable of producing high concentrations of nitric oxide to prevent respiratory tract infections. Nitric oxide produced by macrophages can kill a variety of pathogens, including escherichia coli and staphylococcus aureus, which are associated with common medical device infections. Studies have shown that nM levels of nitric oxide are sufficient to effectively disrupt biofilms formed by a variety of strains. The high molecular material containing the nitric oxide donor can also effectively control bacterial infection, nitric oxide is a free radical gas molecule with extremely short half-life period, drug resistance and toxic and side effects are not easy to generate in the action process, and the disadvantage of the nitric oxide is that the self gas and the short half-life period of nitric oxide are not beneficial to storage and practical use, so that the application of nitric oxide as a bacteriostatic agent or an antibacterial agent of a sanitary product is greatly limited.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an antibacterial material, a preparation method and application thereof, and particularly provides an antibacterial material capable of self-providing nitric oxide, a preparation method thereof and application thereof in preparing sanitary towels or panty liners.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an antibacterial material capable of self-supplying nitric oxide, which includes a base material and a functional material supported on the base material; the functional material includes a NO donor material, a binder, a dispersant, and a water repellent.
The NO donor material in the antibacterial material capable of providing nitric oxide can release NO under the stimulation of light, heat or water vapor, so that the antibacterial material has the functions of inhibiting the growth of microorganisms, sterilizing and resisting viruses; the invention adds adhesive to make NO donor material stably adhere to the substrate material, adds dispersant to ensure that NO donor material is uniformly distributed on the substrate material, and waterproof agent helps NO slow-release behavior, and the four materials complement each other to realize synergistic effect and ensure comfort and air permeability of the antibacterial material, and is especially suitable for being used as raw material for preparing sanitary towel or pad products, and the duration of NO release is kept at about 2-5h under the triggering of water vapor.
Preferably, the functional material comprises 4-7 parts of NO donor material, 1-5 parts of adhesive, 0.1-10 parts of dispersant and 1-30 parts of waterproof agent by mass.
According to the invention, under the condition that the four functional materials in the antibacterial material capable of providing nitric oxide by self are mixed according to the mass parts, the adhesion, stability, dispersion uniformity, NO donor material utilization rate and antibacterial property of the material can achieve better effects. If the number of the adhesive is too large relative to the NO donor material, the hardness of the non-woven fabrics is increased, structural pores among the non-woven fabrics are blocked, the local air permeability of the sanitary towel pad is reduced, and if the number of the adhesive is too small, the total amount of the donor adhered to the base material is reduced, so that the utilization rate of the NO donor is reduced; the excessive part of the dispersing agent relative to the NO donor material can cause the adhesive force of the adhesive to be reduced and the concentration to be attenuated, so that the adhesion effect of the adhesive is weakened, the insufficient part of the dispersing agent can cause the donor not to be completely and uniformly distributed in the solution, the dispersion is unstable, the donor is easy to form sedimentation, the phenomenon that the NO donor is granular when the NO donor is sprayed on the non-woven fabric is caused, and meanwhile, the NO donor is not uniformly distributed on the non-woven fabric; the excessive parts of the waterproof agent relative to the NO donor material can lead the donor not to be completely released from the time of using the sanitary towel by a human body to the time of replacing the NO donor, and the excessive parts of the waterproof agent can lead the NO donor to release NO too quickly and cannot meet the required wearing time in the process of using the sanitary towel by the human body.
The mass portion of the NO donor material can be 4 parts, 5 parts, 6 parts or 7 parts, and any specific point value in the numerical value range can be selected, and the detailed description is omitted.
The mass portion of the waterproof agent can be 1 part, 3 parts, 5 parts, 8 parts, 10 parts, 12 parts, 13 parts, 15 parts, 18 parts, 20 parts, 25 parts or 30 parts, and any specific point value in the numerical value range can be selected, and is not repeated herein.
The mass portion of the adhesive can be 1 part, 2 parts, 3 parts, 4 parts or 5 parts, and any specific point value in the above numerical range can be selected, and is not repeated herein.
The mass portion of the dispersing agent can be 0.1 portion, 0.2 portion, 0.6 portion, 1 portion, 2 portions, 3 portions, 4 portions, 5 portions, 6 portions, 8 portions or 10 portions, and any specific point value in the above numerical value range can be selected, and the description is omitted.
Preferably, the loading of the NO donor material on the substrate material is between 50% and 90%.
The specific selection of the loading rate of the NO donor material on the substrate material in the antibacterial chip is 50-90%, because if the loading rate is too high, the hardness of the finished non-woven fabric is high, the air permeability is reduced, and if the loading rate is too low, the utilization rate of the donor is reduced, and the cost is increased.
Preferably, the base material comprises a non-woven fabric, a melt-blown fabric, a filter cotton, a polypropylene non-woven fabric or a sponge, preferably a non-woven fabric.
The base material in the antibacterial material capable of providing nitric oxide by self can be selected from the types, and the water permeability and the water vapor permeability of the filter cotton, the polypropylene non-woven fabric and the sponge are better and the dispersion uniformity of the functional material on the filter cotton, the polypropylene non-woven fabric and the sponge is poorer by comparing the properties of the base materials by single factors; the melt-blown fabric has poor water permeability and water vapor permeability, and the functional material has poor dispersion uniformity on the melt-blown fabric; the nonwoven fabric is preferably used as the base material because it has a low water permeability, a high water vapor permeability, and a high dispersion uniformity of the functional material on the surface.
Preferably, the NO donor material includes any one or a combination of at least two of sodium nitrosoiron hydride, S-nitroso-N-acetylpenicillamine, S-nitrosoglutathione, azoalkene diol series compounds, or azoalkene diol loaded macromolecules, such as a combination of S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine, a combination of sodium nitrosoiron hydride and azoalkene diol series compounds, and the like, and any combination may be selected, which is not described in detail herein. S-nitrosoglutathione is preferred.
The NO donor material in the antibacterial material capable of self-supplying nitric oxide can be selected from the above types, wherein two materials, namely S-nitrosoglutathione (GSNO) or S-nitroso-N-acetylpenicillamine (SNAP) are preferably used as the NO donor material, the S-nitrosoglutathione powder is in light pink color, and the S-nitroso-N-acetylpenicillamine powder is in light green color, so that the antibacterial functional material is in light pink color or light green color as a whole. If the antibacterial agent is used for preparing the sanitary towel or the sanitary pad, NO can be stimulated to be released under the action of water vapor in the using process, so that microorganisms in the local environment are inhibited, and the color of the sanitary towel or the sanitary pad is gradually changed from light pink or light green to colorless along with the prolonging of the using time, so that the user is prompted to change the residual amount of the antibacterial activity and replace the sanitary towel or the sanitary pad in time.
Compared with two materials, namely S-nitrosoglutathione and S-nitroso-N-acetylpenicillamine, the former is an endogenous substance and has no odor, the latter is not an endogenous substance and has certain odor, and the odor of the latter needs to be removed or masked in other ways when the later is used. Therefore, S-nitrosoglutathione is more preferable as the NO donor material in the antibacterial material that can self-supply nitric oxide according to the present invention.
Preferably, the binder includes any one or a combination of at least two of dextrin, hydroxymethyl cellulose, silica gel, pectin and polyvinyl acetate latex, for example, a combination of dextrin and hydroxymethyl cellulose, a combination of silica gel and pectin, a combination of pectin and polyvinyl acetate latex, and the like, and any other combination may be selected, which is not described in detail herein. Polyvinyl acetate latex is preferred.
Polyvinyl acetate latex is superior to other adhesive types in promoting the ability of the functional material to adhere to the base material.
Preferably, the dispersant includes any one of polyvinyl alcohol, polyethylene glycol, sodium dodecyl sulfate or sodium dodecyl sulfate, or a combination of at least two of them, for example, a combination of polyvinyl alcohol and polyethylene glycol, a combination of sodium dodecyl sulfate and sodium dodecyl sulfate, a combination of polyvinyl alcohol and sodium dodecyl sulfate, etc., and any other combination may be selected, which is not described herein again. A combination of polyvinyl alcohol and sodium lauryl sulfate is preferred.
The combination of polyvinyl alcohol and sodium lauryl sulfate is more pronounced in promoting the uniformity of dispersion of the functional material on the substrate material than other dispersant types.
Preferably, the waterproof agent comprises any one of or a combination of at least two of simethicone, liquid paraffin, solid paraffin, silicone resin, lanolin, natural resin or vaseline; the combination of at least two of the above-mentioned compounds, such as the combination of liquid paraffin and solid paraffin, the combination of dimethicone and silicone resin, the combination of silicone resin and lanolin, etc., can be selected in any combination manner, and is not repeated herein. Natural resins are preferred.
Compared with other waterproof agent types and natural resin, when the antibacterial chip is used in a scene of an antibacterial chip of a sanitary towel pad, the antibacterial chip is better in appearance, free of sticky feeling and falling off, and meanwhile, the antibacterial chip can slowly release NO from a nitric oxide donor after passing through a water system, and has a better waterproof effect.
In a second aspect, the present invention provides a method for preparing an antibacterial material capable of self-supplying nitric oxide, the method comprising:
(1) Mixing NO donor material, adhesive, dispersant and solvent to prepare coating liquid;
(2) And (2) spraying the coating solution prepared in the step (1) onto a substrate material, drying, and finally coating a waterproof agent on the surface of the substrate material to obtain the antibacterial material capable of providing nitric oxide.
The spraying needs to be carried out slowly at a constant speed, and the speed is not too high so as to prevent water molecules of the solution from penetrating through pores of the substrate material and not reaching the retention effect. The preparation method of the antibacterial material capable of self-providing nitric oxide, which is provided by the invention, has the advantages of simple process and easiness in operation, and is suitable for industrial production.
Preferably, the step (1) specifically comprises the following steps:
(a) Mixing a dispersant and a solvent, and stirring to prepare a solution A; mixing the NO donor material with a solvent, stirring, mixing with an adhesive, and stirring to obtain a solution B;
(b) And (B) mixing the solution A and the solution B prepared in the step (a), and stirring to prepare a coating liquid.
The preparation of the solution B in the step (a) influences the visual effect of the antibacterial material after coating, and if the donor material is not fully dissolved in the solvent, pink patches with different sizes can be formed after spraying, so that the overall aesthetic feeling of the material is influenced.
When the antibacterial material is prepared by the method, the specific preparation sequence of the coating liquid ensures the release of the NO donor material, is beneficial to the adhesion of the donor material and the substrate material, and finally exerts the self-cleaning performance of inhibiting microorganisms.
The purpose of said drying is to give better adhesion of the NO donor to the substrate material, and a clean flat table can be selected, which is dried by evaporation in a defined time, and the donor material penetrates into the substrate material, where the binder in the solution functions as a blocking, and locks the donor better to the substrate material. It cannot be exposed to sunlight or dried by strong hot wind, and the sunlight and hot wind can cause the donor to release NO in advance, thereby reducing the effective content of the donor.
Preferably, the drying temperature in step (2) is 20-40 ℃, for example, 20 ℃, 25 ℃, 30 ℃, 35 ℃ or 40 ℃, and other specific values in the range can be selected, and are not described in detail herein.
Preferably, the drying time is 1-3h, for example, 1h, 1.5h, 2h, 2.5h or 3h, and other specific values within the range can be selected, and are not repeated herein.
Preferably, the spraying of the coating solution onto the base material in step (2) is performed 1 to 5 times in total, for example 1, 2, 3, 4 or 5 times.
In a third aspect, the present invention provides a use of the above-mentioned antibacterial material capable of self-supplying nitric oxide in the preparation of a sanitary napkin or a panty liner.
Compared with the prior art, the invention has the following beneficial effects:
the NO donor material in the antibacterial material capable of providing nitric oxide can release NO under the stimulation of light, heat or water vapor, so that the antibacterial material has the functions of inhibiting the growth of microorganisms, sterilizing and resisting viruses; the invention adds adhesive to make NO donor material stably adhere to the substrate material, adds dispersant to ensure that NO donor material is uniformly distributed on the substrate material, and waterproof agent helps NO slow-release behavior, and the four materials complement each other to realize synergistic effect and ensure comfort and air permeability of the antibacterial material, and is especially suitable for being used as raw material for preparing sanitary towel or pad products, and the duration of NO release is kept at about 2-5h under the triggering of water vapor.
When GSNO or SNAP is used as NO donor, if the self-nitric oxide-providing antibacterial material is used to prepare sanitary napkins or pads, the color gradually changes from light pink or light green to colorless as the using time is prolonged, which prompts the user to "residual antibacterial activity" and replace the sanitary napkins or pads in time.
Drawings
FIG. 1 is a graph of the color change of the product prepared in example 1 before and after release of NO;
FIG. 2 is a plate colony chart of the product of example 1 in the case of antibacterial property examination;
FIG. 3 is a plate colony diagram of the kanamycin group during antibacterial property investigation;
FIG. 4 is a plate colony diagram of silver ion groups in antibacterial property investigation;
FIG. 5 is a plate colony diagram of the blank control group in the antibacterial property test.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitation of the present invention.
Example 1
The embodiment prepares an antibacterial material capable of self-providing nitric oxide, which comprises a base material (non-woven fabric) and a functional material loaded on the non-woven fabric; the functional material includes NO donor material (GSNO), binder (polyvinyl acetate latex), dispersant (polyvinyl alcohol and sodium lauryl sulfate), and water repellent (natural resin). The preparation method comprises the following steps:
(1) Mixing 20mg of polyvinyl alcohol, 5mg of sodium dodecyl sulfate and 10mL of water, and stirring at 23 ℃ for 10min to prepare a solution A; mixing 500mg of GSNO with 10mL of water, stirring at 23 ℃ for 5min, mixing with 250mg of polyvinyl acetate latex, and stirring at 23 ℃ for 5min to prepare a solution B;
(2) Mixing the solution A and the solution B prepared in the step (1), and stirring for 5min at 23 ℃ to prepare coating liquid;
(3) Uniformly spraying the coating liquid obtained in the step (2) onto a non-woven fabric with the thickness of 0.185mm, volatilizing and drying, repeating the spraying, and finally volatilizing and drying again, wherein the load rate of GSNO on the non-woven fabric is 83%;
(4) And (3) placing 800mg of a waterproof agent (natural resin) on two sides of the roller, and uniformly coating the waterproof agent on the front side and the back side of the surface of the product obtained in the step (3) to obtain the antibacterial material capable of self-supplying nitric oxide.
Example 2
The embodiment prepares an antibacterial material capable of providing nitric oxide by itself, which comprises a base material (non-woven fabric) and a functional material loaded on the non-woven fabric; the functional materials include NO donor material (SNAP), binder (polyvinyl acetate latex), dispersant (polyvinyl alcohol and sodium lauryl sulfate), and water repellent (natural resin). The preparation method comprises the following steps:
(1) Mixing 10mg of polyvinyl alcohol, 2.5mg of sodium dodecyl sulfate and 5mL of water, and stirring at 23 ℃ for 10min to prepare a solution A; mixing 250mg of SNAP with 2mL of water, stirring at 23 ℃ for 5min, mixing with 125mg of polyvinyl acetate latex, stirring at 23 ℃ for 5min, and finally adding 3mL of anhydrous ethanol serving as an SNAP promoter, and stirring at 23 ℃ for 5min to prepare a solution B;
(2) Mixing the solution A and the solution B prepared in the step (1), and stirring for 5min at 23 ℃ to prepare coating liquid;
(3) Uniformly spraying the coating liquid obtained in the step (2) onto a non-woven fabric with the thickness of 0.185mm, volatilizing and drying, repeating the spraying, and finally volatilizing and drying again, wherein the load rate of SNAP on the non-woven fabric is 84%;
(4) And (3) placing 500mg of a waterproof agent (natural resin) on two sides of the roller to coat the front side and the back side of the product obtained in the step (3), so as to obtain the antibacterial material capable of providing nitric oxide by self.
Example 3
This example prepares an antibacterial material capable of self-supplying nitric oxide, and its structural composition differs from that of example 1 only in that the dispersant (polyvinyl alcohol and sodium lauryl sulfate) is replaced by the same amount of dispersant (sodium lauryl sulfate), and the preparation method comprises the following steps (1): 25mg of sodium lauryl sulfate was mixed with 10mL of water. All other conditions remained unchanged.
Example 4
This example prepares an antimicrobial material capable of self-supplying nitric oxide, and the structural composition of the material is different from that of example 1 only in that the adhesive polyvinyl acetate latex is replaced by an equal amount of adhesive silica gel, and the other conditions are kept the same. The preparation process is as in example 1.
Example 5
This example prepares an antibacterial material capable of self-supplying nitric oxide, and its structural composition is the same as that of example 1. The preparation method is slightly different from that of the example 1:
(1) Mixing 20mg of polyvinyl alcohol, 5mg of sodium dodecyl sulfate, 500mg of GSNO, 250mg of polyvinyl acetate latex and 20mL of water, and stirring at 23 ℃ for 10min to prepare coating liquid;
(2) Uniformly spraying the coating liquid obtained in the step (1) onto a non-woven fabric with the thickness of 0.185mm, volatilizing and drying, repeating the spraying, and finally volatilizing and drying again, wherein the load rate of GSNO on the non-woven fabric is 80%;
(3) And (3) placing 800mg of a waterproof agent (natural resin) on two sides of the roller, and coating the waterproof agent on the front side and the back side of the product obtained in the step (2) to obtain the antibacterial chip.
Comparative example 1
This comparative example prepared an antibacterial material capable of self-supplying nitric oxide, which was different in structural composition from example 1 only in that it did not contain a binder component, and the other conditions were all kept the same. The preparation method comprises the following steps:
(1) Mixing 20mg of polyvinyl alcohol, 5mg of sodium dodecyl sulfate and 10mL of water, and stirring at 23 ℃ for 10min to prepare a solution A; mixing 500mg of GSNO with 10mL of water, and stirring for 5min at 23 ℃ to prepare a solution B;
(2) Mixing the solution A and the solution B prepared in the step (1), and stirring for 5min at 23 ℃ to prepare coating liquid;
(3) Uniformly spraying the coating liquid obtained in the step (2) onto a non-woven fabric with the thickness of 0.185mm, volatilizing and drying, repeating the spraying, and finally volatilizing and drying again, wherein the load rate of GSNO on the non-woven fabric is 47%;
(4) And (4) placing 800mg of a waterproof agent (natural resin) on two sides of the roller to coat the surface of the product obtained in the step (3), so as to obtain the antibacterial material capable of self-providing nitric oxide.
Comparative example 2
This comparative example prepared an antibacterial material capable of self-supplying nitric oxide, which was different in structural composition from example 1 only in that no dispersant component was contained, and the other conditions were kept the same. The preparation method comprises the following steps:
(1) Mixing 500mg of GSNO with 10mL of water, stirring at 23 ℃ for 5min, mixing with 250mg of polyvinyl acetate latex, and stirring at 23 ℃ for 5min to prepare a solution B;
(2) Mixing the solution A and the solution B prepared in the step (1), and stirring for 5min at 23 ℃ to prepare coating liquid;
(3) Uniformly spraying the coating liquid obtained in the step (2) onto a non-woven fabric with the thickness of 0.185mm, volatilizing and drying, repeating the spraying, and finally volatilizing and drying again, wherein the load rate of GSNO on the non-woven fabric is 70%;
(4) And (3) placing 800mg of a waterproof agent (natural resin) on two sides of the roller to coat the surface of the product obtained in the step (3), so as to obtain the antibacterial material capable of self-providing nitric oxide.
Comparative example 3
This comparative example prepared an antibacterial material capable of self-supplying nitric oxide, which was different in structural composition from example 1 only in that it did not contain a water repellent ingredient, and the other conditions were kept the same. The preparation method comprises the following steps:
(1) Mixing 20mg of polyvinyl alcohol, 5mg of sodium dodecyl sulfate and 10mL of water, and stirring at 23 ℃ for 10min to prepare a solution A; mixing 500mg of GSNO with 10mL of water, stirring at 23 ℃ for 5min, mixing with 250mg of polyvinyl acetate latex, and stirring at 23 ℃ for 5min to prepare a solution B;
(2) Mixing the solution A and the solution B prepared in the step (1), and stirring for 5min at 23 ℃ to prepare coating liquid;
(3) Uniformly spraying the coating liquid obtained in the step (2) onto a non-woven fabric with the thickness of 0.185mm, volatilizing and drying, repeating the spraying, and finally volatilizing and drying again, wherein the load rate of GSNO on the non-woven fabric is 83%; obtaining the antibacterial material capable of self-providing nitric oxide.
Evaluation test:
(1) Evaluation of adhesion:
the adhesion of the products obtained in examples 1 to 5 and comparative examples 1 to 3 was examined, and the evaluation method was specifically as follows: based on the prepared sample, after the sample is acted in a kneading mode for different time, the weight change before and after the sample is compared to evaluate the adhesiveness, the adhesiveness is good if the weight is not obviously changed before and after kneading, and the adhesiveness is poor if the weight is obviously changed before and after kneading, and the results are shown in table 1.
TABLE 1
| Group of | Untreated (mg) | Rub for 1min (mg) | Rub for 2min (mg) |
| Example 1 | 80.82 | 80.40 | 80.21 |
| Example 2 | 81.16 | 80.65 | 80.32 |
| Example 3 | 79.49 | 78.57 | 78.02 |
| Example 4 | 79.17 | 78.58 | 77.49 |
| Example 5 | 79.89 | 79.53 | 79.20 |
| Comparative example 1 | 68.84 | 65.97 | 63.95 |
| Comparative example 2 | 76.50 | 75.45 | 74.38 |
| Comparative example 3 | 80.82 | 80.40 | 79.86 |
As can be seen from the data in Table 1: in examples 1 and 2, compared to comparative example 1, the weight of the antibacterial chip did not change significantly before and after rubbing, which indicates that the NO donor can be better adhered to the substrate under the action of the adhesive.
(2) Evaluation of stability:
the stability of the products prepared in the examples 1-5 and the comparative examples 1-3 is examined, and the evaluation method specifically comprises the following steps: in the accelerated test, the samples were placed in a 4 ℃ refrigerator and a 45 ℃ incubator, respectively, and weighed (in mg) at one week intervals, and photographed as shown in tables 2 (4 ℃) and 3 (45 ℃).
TABLE 2
TABLE 3
| Group of | Week 0 (mg) | 4 weeks (mg) | 9 weeks (mg) | 18 weeks (mg) |
| Example 1 | 80.89 | 80.85 | 80.83 | 80.77 |
| Example 2 | 81.02 | 81.00 | 80.98 | 80.95 |
| Example 3 | 79.46 | 79.44 | 79.41 | 79.34 |
| Example 4 | 79.17 | 79.15 | 79.13 | 79.10 |
| Example 5 | 79.90 | 79.85 | 79.60 | 79.40 |
| Comparative example 1 | 68.92 | 68.90 | 68.87 | 68.78 |
| Comparative example 2 | 76.41 | 76.39 | 76.35 | 76.29 |
| Comparative example 3 | 80.79 | 80.68 | 80.54 | 80.20 |
From the data in tables 2 and 3, it can be seen that: the results of accelerated stability tests of the examples and comparative examples show that the front and rear qualities of the pad chip are not significantly changed and the color is not changed in two different temperature environments, which indicates that the nitric oxide donor coated on the surface of the pad chip has good stability and can be stably stored for a long time.
(3) Evaluation of dispersion uniformity:
the products prepared in examples 1 to 5 and comparative examples 1 to 3 were examined for the uniformity of dispersion, and the evaluation method specifically was: since the NO donor has two donors of pink and light green, whether the donors can be uniformly distributed on the non-woven fabric or not can be roughly evaluated through human visual sense. The non-woven fabric of the same volume after spraying was free of specks, uniform in overall color, and free of aggregation of donor particles, and the result was evaluated as good dispersion uniformity, as shown in table 4.
TABLE 4
| Group of | Presence or absence of plaque |
| Example 1 | Non-plaque |
| Example 2 | Non-plaque |
| Example 3 | Small amount of plaque |
| Example 4 | Small amount of plaque |
| Example 5 | Small amount of plaque |
| Comparative example 1 | Non-plaque |
| Comparative example 2 | Multiple plaque |
| Comparative example 3 | Non-plaque |
From the data in table 4, it can be seen that: plaque formation due to the small clumpy mass of donor particles that did not disperse uniformly, actual sensory comparison revealed that example 1 and example 2 were more uniform and no plaques, while comparative example 2 had many plaques.
(4) Evaluation of NO Donor availability:
the results of the NO donor utilization test on the products obtained in examples 1-5 and comparative examples 1-3, wherein the NO donor utilization is the ratio of the actual donor loading of the final product to the donor charge, are shown in Table 5.
TABLE 5
From the data in table 5, it can be seen that: the higher donor usage of examples 1 and 2 compared to comparative example 1 indicates that the presence of the binder effectively increases the usage of the NO donor, increases the amount of donor attached to the substrate, and reduces donor waste.
(5) The investigation of the NO release behavior of the products prepared in the examples 1-5 and the comparative examples 1-3 in the presence of water vapor comprises the following specific steps: the samples were exposed to the aqueous solution under nitrogen for various times: 1h, 2h, 3h and 4h; the solutions in different time periods are taken, the NO release concentration under different time periods is detected by a nitric oxide detection kit, the change of the NO concentration (mu mol) of each group along with the time (h) is recorded, and the results are shown in the table 6.
TABLE 6
| Group of | 1h | 2h | 3h | 4h |
| Example 1 | 18.62 | 38.60 | 55.72 | 57.54 |
| Example 2 | 18.62 | 39.24 | 57.47 | 58.14 |
| Example 3 | 17.92 | 35.12 | 52.08 | 54.63 |
| Example 4 | 17.50 | 36.26 | 51.38 | 53.91 |
| Example 5 | 18.49 | 38.10 | 53.86 | 55.46 |
| Comparative example 1 | 17.22 | 25.90 | 29.96 | 32.48 |
| Comparative example 2 | 16.13 | 30.94 | 44.80 | 48.44 |
| Comparative example 3 | 45.29 | 57.45 | 57.13 | 57.43 |
From the data in table 6, it can be seen that: the embodiment 1 and the embodiment 2 completely release NO in about 3 hours under the simulated water vapor condition, and completely release NO under the normal use time of the pad chip, so as to achieve the long-term bacteriostasis; the product NO of the comparative example 3 is released too fast, reaches the plateau stage within 2 hours, and cannot realize the long-time antibacterial effect.
The product obtained in example 1 was photographed and observed before and after 3 hours of the test, and the results are shown in fig. 1, in which the left side shows the picture of the product before the light, the nonwoven fabric is red, the right side shows the picture of the product after the light, and the red color disappears.
(6) Evaluation of antibacterial Properties
The antibacterial property of the products prepared in the examples 1-5 and the comparative examples 1-3 is examined, and the evaluation method specifically comprises the following steps: according to the experiment, by simulating the application scene of the antibacterial chip on the sanitary towel, the NO donor continuously and slowly releases nitric oxide in water for 3 hours, and the release process is also a color change process, so that the NO donor gradually changes from pink to colorless, and the color can be used as a measurement standard for judging the content of the high polymer material donor and the appearance phenomenon of NO release. Therefore, the non-woven fabrics with the diameter of 6mm in different groups are respectively soaked in the bacterial liquid with the same bacterial quantity for 3 hours, then a certain amount of soaking liquid is extracted to culture and count bacterial colonies, and the antibacterial effect of different groups is judged according to the number of the bacterial colonies. (kanamycin and silver ion were used as positive control groups, a kanamycin solution with a concentration of 1mg/mL was sprayed on a nonwoven fabric and air-dried, and a silver ion nonwoven fabric was purchased; a blank control group was also provided), and the results are shown in Table 7 (three replicates for each group, and the data in the table are the average of the three assays).
TABLE 7
The sterilization conditions of the example 1 group, kanamycin group, silver ion group and blank control group are shown in fig. 2, 3, 4 and 5, respectively, and it can be seen from the figures that: the group of example 1 can effectively kill staphylococcus aureus, the antibacterial rate is as high as 98.2%, which is slightly lower than that of the kanamycin group, and it is worth mentioning that the silver ion protection pad used in the market has almost no antibacterial activity.
The applicant states that the present invention is illustrated by the above examples, but the present invention is not limited to the above examples, i.e. the present invention is not limited to practice of the present invention. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
Claims (12)
1. An antibacterial material capable of self-supplying nitric oxide, which is characterized by comprising a base material and a functional material loaded on the base material; the functional material comprises, by mass, 4-7 parts of NO donor material, 1-5 parts of adhesive, 0.1-10 parts of dispersant and 1-30 parts of waterproofing agent;
the NO donor material comprises S-nitroso-N-acetylpenicillamine and/or S-nitrosoglutathione;
the waterproof agent is natural resin;
the dispersing agent is a combination of polyvinyl alcohol and sodium dodecyl sulfate.
2. The self-nitric oxide-supplying antimicrobial material according to claim 1, wherein said base material comprises a non-woven fabric, a filter cotton or a sponge.
3. The self-nitric oxide donating antimicrobial material of claim 1, wherein said base material is a nonwoven fabric.
4. The self-nitric oxide donating antimicrobial material of claim 1, wherein said NO donor material is S-nitrosoglutathione.
5. The self-nitric oxide donating antimicrobial material of claim 1, wherein said binding agent comprises any one or a combination of at least two of dextrin, hydroxymethyl cellulose, silica gel, pectin, or polyvinyl acetate latex.
6. The self-nitric oxide donating antimicrobial material of claim 1, wherein said adhesive comprises polyvinyl acetate latex.
7. The method of preparing the nitric oxide self-supplying antibacterial material according to any one of claims 1 to 6, comprising:
(1) Mixing NO donor material, adhesive, dispersant and solvent to prepare coating liquid;
(2) And (2) spraying the coating liquid prepared in the step (1) onto a substrate material, drying, and finally coating a waterproof agent on the surface of the substrate material to obtain the antibacterial material capable of self-providing nitric oxide.
8. The method for preparing the antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the step (1) comprises the following steps:
(a) Mixing a dispersant and a solvent, and stirring to prepare a solution A; mixing the NO donor material with a solvent, stirring, mixing with an adhesive, and stirring to obtain a solution B;
(b) And (B) mixing the solution A and the solution B prepared in the step (a), and stirring to prepare a coating liquid.
9. The method for preparing an antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the drying temperature in the step (2) is 20 to 40 ℃.
10. The method for preparing an antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the drying time is 1 to 3 hours.
11. The method for preparing an antibacterial material capable of self-supplying nitric oxide according to claim 7, wherein the spraying of the coating solution onto the base material in the step (2) is performed 1 to 5 times in total.
12. Use of the self-nitric oxide donating antibacterial material of any one of claims 1-6 in the manufacture of a sanitary napkin or panty liner.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1723778A (en) * | 2004-07-20 | 2006-01-25 | 深圳市清华源兴生物医药科技有限公司 | Silver-contg. type antibiotic agent, prepn. method and application thereof |
| CN1730804A (en) * | 2005-08-26 | 2006-02-08 | 上海汽车集团股份有限公司 | Antibacterial fabric and its preparation method |
| CN104927348A (en) * | 2014-03-20 | 2015-09-23 | 北京化工大学 | Antibacterial and anticoagulant polymer material, preparation method and application thereof |
| CN109846113A (en) * | 2018-11-29 | 2019-06-07 | 南京诺全生物医疗科技有限公司 | One kind can be from the nitric oxide production mouth and nose amenities of offer |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1723778A (en) * | 2004-07-20 | 2006-01-25 | 深圳市清华源兴生物医药科技有限公司 | Silver-contg. type antibiotic agent, prepn. method and application thereof |
| CN1730804A (en) * | 2005-08-26 | 2006-02-08 | 上海汽车集团股份有限公司 | Antibacterial fabric and its preparation method |
| CN104927348A (en) * | 2014-03-20 | 2015-09-23 | 北京化工大学 | Antibacterial and anticoagulant polymer material, preparation method and application thereof |
| CN109846113A (en) * | 2018-11-29 | 2019-06-07 | 南京诺全生物医疗科技有限公司 | One kind can be from the nitric oxide production mouth and nose amenities of offer |
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