CN111961747B - Method for assisting in identifying sugar content of watermelon fruit, SNP marker and KASP marker - Google Patents
Method for assisting in identifying sugar content of watermelon fruit, SNP marker and KASP marker Download PDFInfo
- Publication number
- CN111961747B CN111961747B CN202010945909.XA CN202010945909A CN111961747B CN 111961747 B CN111961747 B CN 111961747B CN 202010945909 A CN202010945909 A CN 202010945909A CN 111961747 B CN111961747 B CN 111961747B
- Authority
- CN
- China
- Prior art keywords
- watermelon
- genotype
- sugar content
- sequence
- identifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
The invention discloses a method for assisting in identifying the sugar content of watermelon fruits, an SNP marker and a KASP marker. The invention discloses a method for identifying or assisting in identifying the sugar content of watermelon fruits, which comprises the following steps: detecting the genotype of the watermelon to be detected based on the specific SNP; the sugar content of the fruit of the CC genotype watermelon is higher than that of the AA genotype watermelon. The specific SNP is (a) or (b) as follows: (a) 18785631 th nucleotide on chromosome 2 of watermelon genome; (b) the 52 th nucleotide of the DNA molecule shown in the sequence 1 of the sequence table in the watermelon genome. The method can realize the initial screening of the watermelon variety, achieve the aim of detecting the sugar content of the watermelon fruit in the seedling stage, greatly shorten the breeding period, and have important theoretical and practical significance.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a method for assisting in identifying sugar content of watermelon fruits, an SNP marker and a KASP marker.
Background
Watermelon (Citrullus lanatus) belongs to important cucurbitaceae crops, and the yield of Chinese watermelons accounts for about 60% of the annual global yield (http:// faostat. fao. org). Watermelon is an important horticultural crop with international competitiveness and great economic growth space in china. The watermelons originate in Africa, and the sugar content of wild watermelons in Africa is low and only 1-3 degrees. The sugar content of modern watermelon fruits can reach 10-14 degrees. The high-sugar watermelon is the main character of the human breeding.
In watermelon germplasm resources stored in a watermelon germplasm resource library (http:// www.ars-grin. gov /), genes with excellent traits such as disease resistance and the like mostly exist in wild and semi-wild low-sugar materials. Since fruit sugar is composed of raffinose in leaves, it is stored in the fruit as sucrose through a complex network of transport and metabolism. Due to linkage drag of unfavorable characters, under the condition of no molecular marker-assisted breeding sugar content, breeding breeders dare not to easily use wild and semi-wild disease-resistant excellent resources to improve the disease resistance of parents. The early-stage screening of the variety is carried out by identifying the molecular marker closely linked with the sugar content of the watermelon fruit, the aim of molecular assisted breeding is achieved, the breeding period can be greatly shortened, the breeding efficiency is improved, and the aim of applying low-sugar wild excellent resources to the molecular improvement of main cultivars is fulfilled.
Disclosure of Invention
The invention aims to provide a method for assisting in identifying the sugar content of watermelon fruits, an SNP marker and a KASP marker.
The invention provides an application of a substance for detecting specific SNP in identification or auxiliary identification of the sugar content of watermelon fruits.
The invention also protects the application of the substance for detecting the specific SNP in watermelon breeding.
The invention also discloses a method for identifying or assisting in identifying the sugar content of the watermelon fruit, which comprises the following steps: detecting the genotype of the watermelon to be detected based on the specific SNP; the sugar content of the fruit of the CC genotype watermelon is higher than that of the AA genotype watermelon.
The invention also provides a method for identifying or assisting in identifying the sugar content of the watermelon fruit, which comprises the following steps: detecting the genotype of the watermelon to be detected based on the specific SNP; the CC genotype watermelon is or is candidate for a watermelon with a high sugar phenotype, and the AA genotype watermelon is or is candidate for a watermelon with a low sugar phenotype.
The invention also provides a watermelon breeding method, which comprises the following steps: detecting the genotype of the watermelon to be detected based on the specific SNP; and (3) selecting the CC genotype watermelon for breeding.
The invention also protects a specific DNA molecule which is shown as a sequence 1 in a sequence table.
The invention also protects the application of the specific DNA molecule in identification or auxiliary identification of the sugar content of the watermelon fruit.
The invention also protects a specific primer group which consists of a primer FAM-TST2-A1, a primer HEX-TST2-A2 and a primer TST 2-C.
The invention also protects the application of the specific primer group, which is (c) or (d) as follows:
(c) identifying or assisting in identifying the sugar content of the watermelon fruits;
(d) and (5) breeding the watermelon.
Any one of the above substances for detecting a specific SNP is specifically the specific primer pair.
The method for detecting the genotype of the watermelon to be detected based on the specific SNP specifically comprises the following steps: and (3) amplifying the target fragment with the specific SNP by taking the genome DNA of the watermelon to be detected as a template, and then sequencing.
The method for detecting the genotype of the watermelon to be detected based on the specific SNP specifically comprises the following steps: using the genomic DNA of the watermelon to be tested as a template, and carrying out KASP-based genotyping.
The method for detecting the genotype of the watermelon to be detected based on the specific SNP specifically comprises the following steps: and (3) carrying out KASP by using the specific primer group by using the genome DNA of the watermelon to be detected as a template.
The method for detecting the genotype of the watermelon to be detected based on the specific SNP specifically comprises the following steps:
(1) using the genome DNA of the watermelon to be detected as a template, and adopting the specific primer group to carry out KASP;
(2) and (3) after the step (1) is finished, performing fluorescence scanning to determine the genotype of the watermelon to be detected.
In the reaction system of KASP, the concentrations of FAM-TST2-A1, HEX-TST2-A2 and TST2-C were all 0.5 mM.
Reaction procedure of KASP: pre-denaturation at 94 ℃ for 15 min; denaturation at 94 deg.C for 20s, 61-55 deg.C (touch down program is selected, and each cycle is reduced by 0.6 deg.C) for 1min, 10 cycles; denaturation at 94 ℃ for 20s, renaturation & elongation at 55 ℃ for 1min, 26 cycles.
FAM, blue (focused on the X-axis), represents the CC genotype.
HEX, red (focusing on the Y axis), represents AA genotype.
The primer FAM-TST2-A1 has the 22 nd to 49 th nucleotides in the sequence 2 of the sequence table;
the primer HEX-TST2-A2 has the 22 nd to 51 th nucleotides in the sequence 3 of the sequence table;
the primer TST2-C is shown as a sequence 4 in the sequence table.
The primer FAM-TST2-A1 is a single-stranded DNA molecule shown in a sequence 2 in a sequence table.
The primer HEX-TST2-A2 is a single-stranded DNA molecule shown in a sequence 3 of a sequence table.
The primer TST2-C is specifically a single-stranded DNA molecule shown in a sequence 4 in a sequence table.
The breeding target of the watermelon breeding is to cultivate the watermelon with high sugar phenotype.
The breeding target of any one of the watermelon breeding is to improve the sugar content of watermelon fruits.
The sugar content of the fruit of the CC genotype watermelon is higher than that of the AA genotype watermelon.
The CC genotype watermelon is or is candidate for a watermelon with a high sugar phenotype, and the AA genotype watermelon is or is candidate for a watermelon with a low sugar phenotype.
Watermelon with high sugar phenotype: the fruit contains sugar of 5Brix or more.
Watermelon with low sugar phenotype: the fruit contains less than 5Brix of sugar.
Any one of the specific SNPs is as follows (a) or (b):
(a) 18785631 th nucleotide on chromosome 2 of watermelon genome;
(b) the 52 th nucleotide of the DNA molecule shown in the sequence 1 of the sequence table in the watermelon genome;
the specific SNP is A/C polymorphism.
In scientific research and practice in the field of watermelon breeding, a Single Nucleotide Polymorphism (SNP) marker linked with the sugar content of watermelon fruits and a method for identifying the marker by using competitive Allele Specific PCR (KASP) are required.
The invention develops an SNP marker related to the sugar content of watermelon fruits, identifies the sugar content of the fruits of 111 watermelon natural population materials, detects the linkage condition of the SNP marker and the sugar content of the watermelon fruits by utilizing a KASP marker, and finds that the watermelon varieties can be initially screened by SNP polymorphism or genotype. The method can realize the initial screening of the watermelon varieties, achieves the aim of detecting the sugar content of the watermelon fruits at the seedling stage, greatly shortens the breeding period, and has important theoretical and practical significance.
Drawings
FIG. 1 is a graph showing the results of example 2.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, and the examples are given only for illustrating the present invention and not for limiting the scope of the present invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.
The experimental procedures in the following examples, unless otherwise indicated, are conventional and are carried out according to the techniques or conditions described in the literature in the field or according to the instructions of the products. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Unless otherwise stated, the quantitative tests in the following examples were carried out in triplicate, and the results were averaged.
The germplasm resources of 111 watermelons used in the examples are shown in table 1 and can be obtained from a germplasm resource library of vegetable research center of agriculture and forestry academy of sciences of Beijing. The germplasm resource numbers in the subsequent tables are all in one-to-one correspondence with the germplasm resource numbers in the table 1.
TABLE 1
Example 1 discovery of SNP marker and KASP marker relating to sugar content in watermelon fruit
Test materials: 111 kinds of watermelon germplasm resources.
First, detection of sugar content in fruit
All test materials were tested in parallel: the sugar content of the mature fruit was measured using a hand-held refractometer (ATAGO co., ltd., Tokyo, Japan) grown continuously at the same site for two years. The average value of the fruits of a plurality of plants in two years is taken as the sugar content of the fruits of each test material. The fruit contains sugar in an amount of 5Brix or more, and is defined as a high-sugar phenotype. The fruit has a sugar content of less than 5Brix and is defined as a low sugar phenotype.
The results of the sugar content in the fruit are shown in column 2 of Table 2. 78 parts of the test material had a high sugar phenotype and 33 parts of the test material had a low sugar phenotype.
TABLE 2
Second, discovery of SNP associated with sugar content in fruit
1. Whole genome association analysis
The TASSEL3.0 software is used for carrying out whole genome association analysis on the test material, and as a result, the fruit sugar content gene is positioned at the CHR2-18785631 position and SNP on both sides of the CHR2-18785631 position.
2. Discovery of SNPs related to sugar content in fruit
Comparing genome sequences of the watermelon natural population materials in a primary positioning gene interval by using a test material whole-gene association analysis result to obtain candidate SNP sites highly conforming to the phenotypic characters of the watermelon natural population materials; searching SNP loci closely linked with the sugar content of the fruits in the natural population material of the watermelon, and finding that one SNP locus (A → C) of the No. 2 chromosome is most related to the sugar content character of the fruits.
The SNP found above was named as a specific SNP located in the watermelon genome (http:// cucurbitgenomics.org/V2) at position 18785631 (corresponding to position 52 in sequence 1 of the sequence listing, M represents A or C) on chromosome 2, which is an A/C polymorphism.
Based on the genotype of the specific SNP, the genotype of the test material is shown in column 3 of Table 2 as AA genotype or CC genotype. The sugar content of the fruit of the CC genotype watermelon is higher than that of the AA genotype watermelon.
Design of three, KASP labeled primer group
And designing a complete set of KASP primer sets according to the specific SNP. The KASP primer group consists of 2 upstream primers (FAM-TST2-A1 and HEX-TST2-A2) and 1 universal downstream primer (TST 2-C).
FAM-TST2-A1 (SEQ ID NO: 2 of the sequence Listing):
5′-GAAGGTGACCAAGTTCATGCTCTCAAAACCCTCTTTAAATTTTACTGAC-3′；
HEX-TST2-A2 (sequence 3 of the sequence table):
5′-GAAGGTCGGAGTCAACGGATTAACTCAAAACCCTCTTTAAATTTTACTGAA-3′；
TST2-C (SEQ ID NO: 4 of the sequence Listing): 5'-GAATTATCACACTGACCATTGTTTCACTC-3' are provided.
Example 2 detection of genotype and identification of sugar content in fruits Using KASP primer set
Test materials: 111 kinds of watermelon germplasm resources.
1. Taking the plant leaves of the test material, and extracting genome DNA.
2. Using the genomic DNA obtained in step 1 as a template, and performing genotyping by using a KASP primer set through KASP (Kompetitive Allle-Specific PCR).
The SNP genotyping procedure was carried out according to the experimental procedure of KASP technique provided by LGC company, and the reagents, consumables and instruments used below, which are not specifically described, were all provided by LGC company, including reagent amounts, usage, and the whole experimental procedure were carried out according to the LGC company's instruction KASP user guide and manual (www.lgcgenomics.com).
Reaction system (total volume 2 μ l): template DNA (DNA content 50-100ng), 2 XKASP reaction mix 1. mu.l, mixed primer 0.2. mu.l, and the balance ddH 2 And (O). The effective components provided by the mixed primer are FAM-TST2-A1, HEX-TST2-A2 and TST 2-C. In the reaction system, the concentrations of FAM-TST2-A1, HEX-TST2-A2 and TST2-C were all 0.5 mM.
The 2 XKASP reaction Mix, also called KASP 2 XMaster Mix, is a product of LGC company (product number KBS-1016-002). The 2 xKASP reaction mix comprises a fluorescent probe A, a fluorescent probe B, a quenching probe A and a quenching probe B, as well as high-fidelity Taq enzyme, dNTP and the like. The sequence of the fluorescent probe A is 5'-GAAGGTGACCAAGTTCATGCT-3', and the 5 ' end is connected with 1 fluorophore FAM; the sequence of the fluorescent probe B is 5'-GAAGGTCGGAGTCAACGGATT-3', and the 5 ' end is connected with 1 fluorescent group HEX; the sequence of the quenching probe A is 5'-AGCATGAACTTGGTCACCTTC-3', and the 3 ' terminal is connected with a quenching group BHQ; the sequence of the quenching probe B is 5'-AATCCGTTGACTCCGACCTTC-3', and the 3 ' terminal is connected with a quenching group BHQ.
Reaction procedure: pre-denaturation at 94 ℃ for 15 min; denaturation at 94 deg.C for 20s, 61-55 deg.C (touch down program is selected, and each cycle is reduced by 0.6 deg.C) for 1min, 10 cycles; denaturation at 94 ℃ for 20s, renaturation & elongation at 55 ℃ for 1min, 26 cycles.
3. And (3) after the step (2) is finished, when the temperature of the PCR amplification product is reduced to be below 40 ℃, scanning and reading a fluorescence value through FAM and HEX light beams of a microplate reader (reading value is observed when FAM fluorescence is at the wavelength of 485nm of exciting light and 520nm of emitted light; reading value is observed when HEX fluorescence is at the wavelength of 528nm of exciting light and 560nm of emitted light), and judging the genotype according to the color of a fluorescence signal.
The results are shown in FIG. 1. The results are presented as scatter plots, each dot representing a sample, with the same genotype of dots concentrated in one region. The red fluorescence signal (concentrated near the Y-axis) represents the AA genotype. The blue fluorescence signal (concentrated around the X-axis) represents the CC genotype. The green fluorescent signal represents the AC genotype.
The results of the detection were 100% consistent with the genotype results in Table 2. Watermelon of CC genotype has high sugar phenotype. The AA genotype watermelons all have a low sugar phenotype.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific examples, it will be appreciated that the invention may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. The use of some of the essential features is possible within the scope of the claims attached below.
Sequence listing
<110> agriculture, forestry, and scientific colleges in Beijing
Jingyan Yinong (Beijing) seed industry science and technology Co., Ltd
<120> method for auxiliary identification of sugar content of watermelon fruit, SNP marker and KASP marker
<130> GNCYX202232
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 105
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
ccactcaaca catccaagtt gcaactcaaa accctcttta aattttactg amtaaagtta 60
attgcgagtg aaacaatggt cagtgtgata attcttcgtt ttgaa 105
<210> 2
<211> 49
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
gaaggtgacc aagttcatgc tctcaaaacc ctctttaaat tttactgac 49
<210> 3
<211> 51
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
gaaggtcgga gtcaacggat taactcaaaa ccctctttaa attttactga a 51
<210> 4
<211> 29
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
gaattatcac actgaccatt gtttcactc 29
Claims (4)
1. A method for identifying or assisting in identifying the sugar content of watermelon fruits comprises the following steps: detecting the genotype of the watermelon to be detected based on the specific SNP molecular marker; the sugar content of the watermelon with the CC genotype of the specific SNP molecular marker is higher than that of the watermelon with the AA genotype; the specific SNP molecular marker is the 52 th nucleotide of the DNA molecule shown in the sequence 1 of the sequence table, and is A or C.
2. A method for identifying or assisting in identifying the sugar content of watermelon fruits comprises the following steps:
detecting the genotype of the watermelon to be detected based on the specific SNP molecular marker; the watermelon with the genotype of CC of the specific SNP molecular marker is or is selected as a watermelon with a high sugar phenotype, and the watermelon with the genotype of AA of the specific SNP molecular marker is or is selected as a watermelon with a low sugar phenotype;
the specific SNP molecular marker is the 52 th nucleotide of a DNA molecule shown in a sequence 1 of a sequence table, and is A or C;
watermelon with high sugar phenotype: the sugar content of the fruit is more than or equal to 5 Brix; watermelon with low sugar phenotype: the fruit contains less than 5Brix of sugar.
3. A method for breeding watermelon comprises the following steps: detecting the genotype of the watermelon to be detected based on the specific SNP molecular marker; selecting the watermelon with the genotype of CC of the specific SNP molecular marker for breeding; the specific SNP molecular marker is the 52 th nucleotide of a DNA molecule shown in a sequence 1 of a sequence table, and is A or C; the breeding target of the watermelon breeding is to improve the sugar content of watermelon fruits.
4. The application of the specific primer pair is (c) or (d):
(c) identifying or assisting in identifying the sugar content of the watermelon fruits;
(d) breeding the watermelon; the breeding target of the watermelon breeding is to improve the sugar content of watermelon fruits;
the specific primer pair detects the genotype of a specific SNP molecular marker, and the sugar content of the watermelon with the genotype of CC is higher than that of the watermelon with the genotype of AA; the specific SNP molecular marker is the 52 th nucleotide of a DNA molecule shown in a sequence 1 of a sequence table, and is A or C;
the specific primer pair consists of a primer FAM-TST2-A1, a primer HEX-TST2-A2 and a primer TST 2-C; the primer FAM-TST2-A1 is a single-stranded DNA molecule shown in a sequence 2 of a sequence table, the primer HEX-TST2-A2 is a single-stranded DNA molecule shown in a sequence 3 of the sequence table, and the primer TST2-C is a single-stranded DNA molecule shown in a sequence 4 of the sequence table.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010945909.XA CN111961747B (en) | 2020-09-10 | 2020-09-10 | Method for assisting in identifying sugar content of watermelon fruit, SNP marker and KASP marker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010945909.XA CN111961747B (en) | 2020-09-10 | 2020-09-10 | Method for assisting in identifying sugar content of watermelon fruit, SNP marker and KASP marker |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111961747A CN111961747A (en) | 2020-11-20 |
| CN111961747B true CN111961747B (en) | 2022-09-06 |
Family
ID=73392837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010945909.XA Active CN111961747B (en) | 2020-09-10 | 2020-09-10 | Method for assisting in identifying sugar content of watermelon fruit, SNP marker and KASP marker |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111961747B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113969323B (en) * | 2021-10-14 | 2022-08-02 | 广东省农业科学院蔬菜研究所 | SNP molecular marker related to luffa melon length and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194444A (en) * | 2013-04-11 | 2013-07-10 | 北京市农林科学院 | SNP (single nucleotide polymorphism) site and CAPS (cleaved amplified polymorphic sequence) mark interlocked with citrullus lanatus fruit bitter taste gene Bt (bitterness) |
| CN108192990A (en) * | 2018-02-01 | 2018-06-22 | 中国农业科学院郑州果树研究所 | SNP marker relevant with watermelon pericarp background color and its application |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105506149B (en) * | 2016-01-27 | 2019-03-05 | 北京市农林科学院 | The chain SNP site of Watermelon Fruit sucrose accumulation gene STP1 and CAPS label |
| CN109152346B (en) * | 2016-02-05 | 2022-03-08 | 瑞克斯旺种苗集团公司 | QTLs conferring resistance to potyvirus in watermelons |
| CN110656199B (en) * | 2019-10-24 | 2022-07-05 | 北京市农林科学院 | SNP marker and KASP marker related to watermelon fruit raffinose unloading capacity |
| CN111334597B (en) * | 2020-01-23 | 2022-07-19 | 北京市农林科学院 | SNP (Single nucleotide polymorphism) site and KASP (Kaempferi protein) marker for detecting powdery mildew resistance of watermelon and application thereof |
-
2020
- 2020-09-10 CN CN202010945909.XA patent/CN111961747B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103194444A (en) * | 2013-04-11 | 2013-07-10 | 北京市农林科学院 | SNP (single nucleotide polymorphism) site and CAPS (cleaved amplified polymorphic sequence) mark interlocked with citrullus lanatus fruit bitter taste gene Bt (bitterness) |
| CN108192990A (en) * | 2018-02-01 | 2018-06-22 | 中国农业科学院郑州果树研究所 | SNP marker relevant with watermelon pericarp background color and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111961747A (en) | 2020-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111394506B (en) | Wheat molecular marker and application thereof in identifying wheat grain weight characteristics | |
| CN110295251B (en) | SNP molecular marker linked with wheat effective tillering number QTL and application thereof | |
| CN111733281B (en) | Molecular marker for identifying peroxidase activity of wheat grains and application thereof | |
| CN112080582A (en) | KASP molecular marker closely linked with spike length major QTL locus and application thereof | |
| CN110205398B (en) | KASP marker primer for detecting wheat high molecular weight glutelin Dy10-m619SN subunit and application | |
| CN111961750A (en) | KASP primer for detecting tomato yellow leaf curl virus disease resistance gene Ty-1 and application thereof | |
| CN111961749B (en) | KASP primer for detecting tomato yellow leaf curl virus disease-resistant genes Ty-3 and Ty-3a and application thereof | |
| CN109609687B (en) | KASP marker primer combination for detecting watermelon fusarium wilt resistance and application thereof | |
| CN111961747B (en) | Method for assisting in identifying sugar content of watermelon fruit, SNP marker and KASP marker | |
| CN112266975B (en) | Primer group and kit for detecting KASP marker related to POD activity of wheat grains and application of kit | |
| CN113604596A (en) | KASP primer for detecting cucumber small zucchini yellow mosaic virus disease resistance gene zym and application thereof | |
| CN114606332A (en) | SNP (Single nucleotide polymorphism) site and Hf-KASP1 marker for judging pulp hardness of watermelon and application thereof | |
| CN107287210B (en) | Rice appearance quality gene qAQ7 and molecular marking method and application thereof | |
| CN113736866B (en) | SNP locus combination for detecting tomato yellow leaf curl virus resistance and application thereof | |
| CN112779348B (en) | Major QTL site of ear number per unit area of wheat, KASP primer closely linked with major QTL site and application of KASP primer | |
| CN110656199B (en) | SNP marker and KASP marker related to watermelon fruit raffinose unloading capacity | |
| CN111647677B (en) | Molecular marker closely linked with wheat grain filling rate QTL QGfr. sicau-6D and application | |
| CN113528703A (en) | Development and application of KASP molecular marker of rice blast resistance gene Pid3-A4 | |
| CN113355443B (en) | Molecular marker Oil-11-6708663 for assisting in identifying high and low soybean Oil content, kit and method | |
| CN113699273B (en) | SNP locus combination for detecting resistance of tomato root-knot nematode and application thereof | |
| CN113481318B (en) | KASP primer for detecting powdery mildew disease-resistant gene mlo of cucumber and application thereof | |
| CN110904261B (en) | Molecular marker linked with wheat effective tillering number QTL (quantitative trait locus) and application of molecular marker | |
| KR102273447B1 (en) | A set of SNP molecular markers for discriminating radish accessions using KASP assay and uses thereof | |
| CN117887895A (en) | KASP molecular marker for pumpkin fruit length character and application thereof | |
| Chaithra et al. | Development of sex-linked PCR markers for gender identification in papaya |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |