CN111960870A - Method for producing microbial fertilizer by using whisky distillation residual liquid - Google Patents
Method for producing microbial fertilizer by using whisky distillation residual liquid Download PDFInfo
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- CN111960870A CN111960870A CN202010780557.7A CN202010780557A CN111960870A CN 111960870 A CN111960870 A CN 111960870A CN 202010780557 A CN202010780557 A CN 202010780557A CN 111960870 A CN111960870 A CN 111960870A
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- stage
- residual liquid
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- whisky
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a method for producing microbial fertilizer by whisky distillation residual liquid, which comprises two stages of propagation, wherein the first stage is 220L in weight and the second stage is 1000L in weight. After the whisky distillation is finished, introducing 180-220L of residual liquid into a first-stage propagation tank, adjusting the pH to 5.5 by using NaOH solution, heating for disinfection, simultaneously filling sterile air for 10min every 3 hours until the pH value is reduced to 3.5, and detecting the number of thalli; when the thallus content reaches 10 hundred million/ml, firstly adding 1000L of residual liquid of 900 plus materials into the secondary propagation tank, adjusting the pH value to 5.5, cooling to 30 ℃, then pressing all culture solution in the primary culture tank into the secondary propagation tank, culturing for 3-4 days by the same method as the primary tank, finally, filling sterile air for 30min every day, aerating for 7 days, and then bottling, warehousing and selling. The invention not only reduces the manufacturing cost of the culture medium, does not need to purchase materials such as syrup, bean cakes and the like, but also changes waste into valuable, saves resources and avoids environmental pollution caused by discharging residual liquid.
Description
Technical Field
The invention relates to the technical field of microbial fertilizer production, in particular to a method for producing a microbial fertilizer by using whisky distillation residual liquid.
Background
In recent years, microbial inoculum fertilizers are widely applied, and comprise growth promoting inoculants, mycorrhizal inoculants, bioremediation inoculants, rhizobium inoculants, azotobacter inoculants, phosphate solubilizing inoculants, silicate inoculants, photosynthetic bacteria inoculants and the like. The fertilizer has the important functions of improving the yield, improving the quality of crops, enhancing the stress resistance of the crops, improving the utilization rate of the fertilizer, improving the soil nutrient and the like. The microbial agent fertilizer is widely popularized by the nation at present due to the characteristics of effectiveness, safety, environmental protection and no side effect.
The microbial inoculum fertilizer can be divided into liquid, powder and granular according to the formulation, the liquid microbial inoculum is generally prepared by an industrialized culture method, a liquid culture medium is added with strains and is produced in a step-by-step amplification mode, and the main component of the culture medium is saccharide and simultaneously contains certain trace components such as N, P. The preparation method of the culture medium in the prior art is to prepare a solution with sugar content of 3-5% by purchasing high-concentration syrup, bean cakes and the like, and the cost is high.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a method for producing microbial fertilizer by using whisky distillation residual liquid, which solves the problem of high preparation cost of a culture medium.
In order to achieve the purpose, the invention adopts the technical scheme that: a process for producing a microbial fertilizer from a whisky distillation residue comprising the steps of:
step 1: distilling whisky with beer, removing ethanol, carbon dioxide and small amount of volatile substances in the distillation residue, wherein the residue contains sugar 4-4.5% and abundant N, P;
step 2: introducing the whisky distillation residual liquid into an expansion culture tank, adding strains, ventilating and culturing, wherein the production process comprises two-stage expansion culture, wherein the first stage is 180-grade 220L, and the second stage is 900-grade 1000L;
and step 3: after whisky distillation is finished, introducing 180-wall 220L residual liquid into a first-stage propagation tank, adjusting the pH to 5.5 by using NaOH solution, heating and sterilizing, wherein the propagation tank is provided with a stirring and oxygenating device and a jacket, introducing steam into the jacket for heating and sterilizing, introducing ice water into the jacket after sterilization, starting stirring and cooling, stopping cooling when the temperature is reduced to 30 ℃, inoculating strains from a strain inoculation port in a sterile operation mode, measuring the pH value every day, after 3-4 days, reducing the pH value to 3.5, and detecting the number of the strains;
and 4, step 4: when the thallus content in the step 3 reaches 10 hundred million/ml, firstly adding 1000L of whisky distillation raffinate into the secondary expansion culture tank, adjusting the pH to 5.5, cooling to 30 ℃, then pressing all the culture solution in the primary culture tank into the secondary expansion culture tank, and culturing for 3-4 days by the same method as the primary expansion culture tank;
and 5: and finally, filling sterile air for 30min every day, aerating for 7 days, and bottling, warehousing and selling.
Further, the remaining whisky distillation liquid in the step 2 is divided into two stages of propagation, wherein the first stage is 200L, and the second stage is 1000L.
Further, in the step 3, the sterile operation mode means that sterile air is filled for 10min every 3 hours.
Compared with the prior art, the invention has the beneficial effects that: the beer contains 2-3% of residual sugar, after the whisky is distilled by the beer, the ethanol, the carbon dioxide and a small amount of volatile substances in the beer are removed, the sugar content of the residual liquid is 4-4.5%, and the residual liquid also contains rich N, P and other components, so the beer is an ideal raw material for culturing the agricultural microbial agent. Introducing the whisky distillation residual liquid into an expanding culture tank, adding strains, ventilating and culturing, after the thallus content meets the requirement, aerating and after-ripening, and filling for sale. In a word, the invention not only reduces the manufacturing cost of the culture medium, does not need to purchase materials such as syrup, bean cakes and the like, but also changes waste into valuable, saves resources and avoids environmental pollution caused by discharging residual liquid.
Detailed Description
The following will be described in detail with reference to the technical solutions in the embodiments of the present invention:
example 1
A method for producing microbial fertilizer by using whisky distillation residual liquid comprises two-stage propagation, wherein the first stage is 180L, and the second stage is 900L. After whisky distillation is finished, introducing 180L of residual liquid into a first-stage propagation tank, adjusting the pH to 5.5 by using NaOH solution, and heating for disinfection; the expanding culture tank is provided with a stirring device, an oxygenating device and a jacket, steam can be introduced into the jacket for heating, ice water can also be introduced for cooling, after disinfection, the ice water is introduced into the jacket, stirring and cooling are started simultaneously, when the temperature is reduced to 30 ℃, cooling is stopped, strains are inoculated from a strain inoculation port in a sterile operation mode, sterile air is filled for 10min every 3 hours, the pH value is measured every day, about 3-4 days, the pH value is reduced to 3.5, and then the number of the strains is detected; when the thallus content reaches 10 hundred million/ml, adding 900L of whisky distillation residual liquid into a secondary propagation tank, adjusting the pH value to 5.5, cooling to 30 ℃, pressing all culture solution in a primary culture tank into the secondary propagation tank, culturing for 3-4 days by the same method as the primary tank, finally, introducing sterile air for 30min every day, aerating for 7 days, and bottling, warehousing and selling.
Product detection index (execute according to GB 20287-2006)
As can be seen from the above table, the production of the microbial fertilizer from the whisky distillation residual liquid meets the national standard required by the GB 20287-2006 agricultural microbial agent.
Example 2
A method for producing microbial fertilizer by using whisky distillation residual liquid comprises two-stage propagation, wherein the first stage is 200L, and the second stage is 1000L. After whisky distillation is finished, introducing 200L of residual liquid into a first-stage propagation tank, adjusting the pH to 5.5 by using NaOH solution, and heating for disinfection; the expanding culture tank is provided with a stirring device, an oxygenating device and a jacket, steam can be introduced into the jacket for heating, ice water can also be introduced for cooling, after disinfection, the ice water is introduced into the jacket, stirring and cooling are started simultaneously, when the temperature is reduced to 30 ℃, cooling is stopped, strains are inoculated from a strain inoculation port in a sterile operation mode, sterile air is filled for 10min every 3 hours, the pH value is measured every day, about 3-4 days, the pH value is reduced to 3.5, and then the number of the strains is detected; when the thallus content reaches 10 hundred million/ml, firstly adding 1000L of whisky distillation residual liquid into a secondary propagation tank, adjusting the pH value to 5.5, cooling to 30 ℃, then pressing all culture solution in a primary culture tank into the secondary propagation tank, culturing for 3-4 days by the same method as the primary tank, finally, introducing sterile air for 30min every day, aerating for 7 days, and bottling, warehousing and selling.
Product detection index (execute according to GB 20287-2006)
As can be seen from the above table, the production of the microbial fertilizer from the whisky distillation residual liquid meets the national standard required by the GB 20287-2006 agricultural microbial agent.
Example 3
A method for producing microbial fertilizer by using whisky distillation residual liquid comprises two stages of propagation, wherein the first stage is 220L, and the second stage is 950L. After whisky distillation is finished, introducing 220L of residual liquid into a first-stage propagation tank, adjusting the pH to 5.5 by using a NaOH solution, and heating for disinfection; the expanding culture tank is provided with a stirring device, an oxygenating device and a jacket, steam can be introduced into the jacket for heating, ice water can also be introduced for cooling, after disinfection, the ice water is introduced into the jacket, stirring and cooling are started simultaneously, when the temperature is reduced to 30 ℃, cooling is stopped, strains are inoculated from a strain inoculation port in a sterile operation mode, sterile air is filled for 10min every 3 hours, the pH value is measured every day, about 3-4 days, the pH value is reduced to 3.5, and then the number of the strains is detected; when the thallus content reaches 10 hundred million/ml, firstly adding 950L of whisky distillation residual liquid into a secondary propagation tank, adjusting the pH value to 5.5, cooling to 30 ℃, then pressing all culture solution in the primary culture tank into the secondary propagation tank, culturing for 3-4 days by the same method as the primary tank, finally, introducing sterile air for 30min every day, aerating for 7 days, and bottling, warehousing and selling.
Product detection index (execute according to GB 20287-2006)
As can be seen from the above table, the production of the microbial fertilizer from the whisky distillation residual liquid meets the national standard required by the GB 20287-2006 agricultural microbial agent.
The foregoing is directed to embodiments of the present application and it is understood that various modifications and enhancements may be made by those skilled in the art without departing from the principles of the application and are intended to be included within the scope of the application.
Claims (3)
1. A method for producing microbial fertilizer by using whisky distillation residual liquid is characterized by comprising the following steps:
step 1: distilling whisky with beer, removing ethanol, carbon dioxide and small amount of volatile substances in the distillation residue, wherein the residue contains sugar 4-4.5% and abundant N, P;
step 2: introducing the whisky distillation residual liquid into an expansion culture tank, adding strains, ventilating and culturing, wherein the production process comprises two-stage expansion culture, wherein the first stage is 180-grade 220L, and the second stage is 900-grade 1000L;
and step 3: after whisky distillation is finished, introducing 180-wall 220L residual liquid into a first-stage propagation tank, adjusting the pH to 5.5 by using NaOH solution, heating and sterilizing, wherein the propagation tank is provided with a stirring and oxygenating device and a jacket, introducing steam into the jacket for heating and sterilizing, introducing ice water into the jacket after sterilization, starting stirring and cooling, stopping cooling when the temperature is reduced to 30 ℃, inoculating strains from a strain inoculation port in a sterile operation mode, measuring the pH value every day, after 3-4 days, reducing the pH value to 3.5, and detecting the number of the strains;
and 4, step 4: when the thallus content in the step 3 reaches 10 hundred million/ml, firstly adding 1000L of whisky distillation raffinate into the secondary expansion culture tank, adjusting the pH to 5.5, cooling to 30 ℃, then pressing all the culture solution in the primary culture tank into the secondary expansion culture tank, and culturing for 3-4 days by the same method as the primary expansion culture tank;
and 5: and finally, filling sterile air for 30min every day, aerating for 7 days, and bottling, warehousing and selling.
2. The method for producing microbial fertilizer from whisky distillation residue as claimed in claim 1, wherein the whisky distillation residue in the step 2 is divided into two stages of propagation, the first stage is 200L, and the second stage is 1000L.
3. The method for producing microbial fertilizer from whiskey distillation residue as claimed in claim 1, wherein in the step 3, the aseptic operation is performed by filling the aseptic air for 10min every 3 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010780557.7A CN111960870A (en) | 2020-08-06 | 2020-08-06 | Method for producing microbial fertilizer by using whisky distillation residual liquid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010780557.7A CN111960870A (en) | 2020-08-06 | 2020-08-06 | Method for producing microbial fertilizer by using whisky distillation residual liquid |
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| CN111960870A true CN111960870A (en) | 2020-11-20 |
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| CN202010780557.7A Pending CN111960870A (en) | 2020-08-06 | 2020-08-06 | Method for producing microbial fertilizer by using whisky distillation residual liquid |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078215A (en) * | 1992-04-30 | 1993-11-10 | 深圳市环境保护设备工程公司 | The treatment process of molasses alcohol waste liquid |
| CN101913919A (en) * | 2010-08-09 | 2010-12-15 | 昆明康禄生物科技有限公司 | Process for treating waste mash of molasses alcohol by adopting compound bacterium two-step fermentation method |
| CN103193521A (en) * | 2013-04-10 | 2013-07-10 | 昆明康禄生物科技有限公司 | Method for preparing fertilizer effect type molasses alcohol waste mash bacterial liquid through triple fermentation |
| CN107382504A (en) * | 2017-08-16 | 2017-11-24 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of method that Liquid Fertilizer is prepared using molasses-spirit raffinate |
| CN107840706A (en) * | 2017-10-17 | 2018-03-27 | 中国科学院生态环境研究中心 | A kind of microbial manure and its application using the production of cassava alcohol waste water |
-
2020
- 2020-08-06 CN CN202010780557.7A patent/CN111960870A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078215A (en) * | 1992-04-30 | 1993-11-10 | 深圳市环境保护设备工程公司 | The treatment process of molasses alcohol waste liquid |
| CN101913919A (en) * | 2010-08-09 | 2010-12-15 | 昆明康禄生物科技有限公司 | Process for treating waste mash of molasses alcohol by adopting compound bacterium two-step fermentation method |
| CN103193521A (en) * | 2013-04-10 | 2013-07-10 | 昆明康禄生物科技有限公司 | Method for preparing fertilizer effect type molasses alcohol waste mash bacterial liquid through triple fermentation |
| CN107382504A (en) * | 2017-08-16 | 2017-11-24 | 广东省生物工程研究所（广州甘蔗糖业研究所） | A kind of method that Liquid Fertilizer is prepared using molasses-spirit raffinate |
| CN107840706A (en) * | 2017-10-17 | 2018-03-27 | 中国科学院生态环境研究中心 | A kind of microbial manure and its application using the production of cassava alcohol waste water |
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Application publication date: 20201120 |
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