CN111956818B - Blue light disinfection and cleaning method for ring-pull tab of pop-top can - Google Patents
Blue light disinfection and cleaning method for ring-pull tab of pop-top can Download PDFInfo
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- CN111956818B CN111956818B CN202010823925.1A CN202010823925A CN111956818B CN 111956818 B CN111956818 B CN 111956818B CN 202010823925 A CN202010823925 A CN 202010823925A CN 111956818 B CN111956818 B CN 111956818B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
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Abstract
The invention discloses a blue light disinfection and cleaning method for a pull ring of a pop can, and belongs to the technical field of food safety packaging and sterilization. The invention relates to a blue light disinfection and cleaning method at a pull ring of a pop can, which comprises the following steps: (1) blue light irradiation is carried out at the pull ring of the pop can; wherein the dose of blue light irradiation is not less than 5J/cm2(ii) a (2) And (3) after irradiation, adopting an adhesive to enable the sterile protective film to reach the ring pull of the pop can irradiated by the blue light in the step (1). According to the invention, blue light irradiates the normally placed ring-pull can, so that pollution bacteria at the ring-pull can are killed non-specifically, the ring-pull can is ensured to be in an aseptic state, meanwhile, the can body of the ring-pull can is ensured not to be adversely affected, and the ring-pull can is ensured to be always in the aseptic state by sticking the protective film, so that the edible safety of consumers is ensured.
Description
Technical Field
The invention relates to a blue light disinfection and cleaning method for a pull ring of a pop can, and belongs to the technical field of food safety packaging and sterilization.
Background
The pop can is the most common metal beverage container in life, can be used for containing various beverages, beer, cold tea, coffee, porridge and the like, has the advantages of convenient transportation, convenient refrigeration, good structural strength, difficult breakage and the like, and not only lays the foundation of the beverage industry, but also plays a role in promoting the aluminum product industry.
Pop-top can structures on the market are basically the same at present, the difference point is mainly in the difference of capacity, can diameter, can height and material, mainly go on through the pull ring when opening, the pull ring structure of current pop-top can divide into embedded pull ring structure and lifts formula pull ring structure outward, nevertheless no matter embedded or lift formula outward, all there is sanitary problem, most pop-top cans all expose outside promptly wholly, very easily be polluted by microorganism in transportation and storage process, especially at the pull ring opening part, because its design is located jar body top groove, so be infected with the possibility of breeding the bacterium bigger. In recent years, there have been reports of food poisoning caused by microbial contamination on a tab due to contact with media such as dust or insects after a number of consumers purchase canned beverages.
The bacteria easy to breed at the pull ring of the pop can mainly comprise escherichia coli, staphylococcus aureus, salmonella, listeria monocytogenes, campylobacter jejuni and vibrio parahaemolyticus. Among them, food poisoning caused by staphylococcus aureus is a food-borne disease caused by exotoxin or endotoxin produced in food. Salmonella-induced food poisoning is a bodily hazard due to the pathogenic bacteria that can pass through the gastrointestinal mucosa, leading to cell death.
The existing bacteriostasis or sterilization method aiming at the pollution bacteria at the pull ring of the pop can comprises the following steps: 1) the high-temperature instantaneous sterilization technology has the defect that the tank body is easily adversely affected; 2) the wiping or the water washing can cause unclean wiping or secondary pollution, and consumes manpower and material resources; 3) the protective agent is coated on the pull ring, which has a certain effect, but causes the problems of inconvenient refrigeration or easy bringing into the mouth of a consumer, and the cost is high.
In view of these problems, there is a need to develop a new sterilization method for a pull-tab of a can, which has the following features: 1) non-specific sterilization; 2) the quality and the safety of the pull ring of the pop can are not damaged, and the color, the aroma and the taste of food in the pop can are not negatively influenced; 3) safe operation, simple method and low equipment requirement.
Disclosure of Invention
In order to solve at least one problem, the invention provides a blue light disinfection and cleaning method at a pull ring of a pop can. Sterilizing and curing sealing operation is carried out by using blue light with specific wavelength (462-480nm), and the pull ring of the pop can is sterilized and kept in an aseptic state; and guarantee is provided for the subsequent storage and transportation of pop can food until the safety of consumers.
The invention aims to provide a blue light disinfection and cleaning method at a pull ring of a pop can, which comprises the following steps:
(1) blue light irradiation is carried out at the pull ring of the pop can; wherein the dose of blue light irradiation is not less than 5J/cm2；
(2) And (3) after irradiation is finished, adhering the sterile protective film to the ring pull of the pop can irradiated by the blue light in the step (1) by adopting an adhesive.
In one embodiment of the present invention, the can is a room temperature or refrigerated can.
In one embodiment of the present invention, the wavelength of the blue light is 462-480 nm.
In one embodiment of the present invention, the irradiation dose of the blue light is 5 to 20J/cm2。
In one embodiment of the invention, the light source of blue light comprises an LED lamp or a fluorescent lamp.
In one embodiment of the invention, the pull tab of the pop can refers to a circular area 2 cm around the pull tab opening.
In one embodiment of the present invention, the binder is a blue light curing binder that achieves curing of a polymerization system through chemical reaction of excited state molecules using blue light waves as an energy source.
In one embodiment of the invention, the parameters of the blue light cure of the adhesive are: the blue light wavelength is 430-485nm, and the curing time is 60 seconds.
In one embodiment of the invention, the binder is poly (glycerol sebacate).
In one embodiment of the invention, the sterile protective film is a PET film, is transparent and has a thickness of 0.1mm-0.25 mm.
In one embodiment of the present invention, the can may be a beverage, beer, herbal tea, coffee, porridge can.
The invention has the beneficial effects that:
(1) various pollution bacteria at the pull ring of the pop can be killed by the irradiation of 462-480nm blue light, and the blue light can generate peroxide by exciting free porphyrin substances in bacteria to damage the cell membrane structure of the bacteria, so that the bacteria die, the treatment time is short, the efficiency is high, unpleasant gas cannot be generated, and the pop can body cannot be damaged.
(2) The pop can body can be refrigerated at low temperature or processed at room temperature, the sterilization operation process has no requirement on the placement environment, and the operation process has no harm to the safety of operators.
(3) The adhesion of the protective film at the pull ring after blue light irradiation ensures the sterile state of the pull ring of the subsequent pop can in the transportation and storage processes.
(4) The blue light wave is used as an energy source, the solidification of a polymerization system is realized through the chemical reaction of excited molecules, and the protective film is stuck to the pull ring, so that the curing is fast, the gluing is convenient, the time is short, the bonding strength is high, and the fine adjustment is easy to realize.
(5) The adhesive, namely the polysebacic acid glyceride is a hydrophobic polymer which has good biocompatibility and is biodegradable, and is safe and harmless to human bodies.
(6) The protective film is made of PET, the PET is a copolymer of polyester, the protective film has the characteristics of high hardness, high transparency and high temperature resistance, the adhesive surface can be provided with glue or has various choices of static electricity and the like, the protective film is the most excellent protective film raw material at present, and the protective film is widely applied to multiple fields.
(7) According to the invention, blue light irradiates the normally placed ring-pull can, so that pollution bacteria at the ring-pull can are killed non-specifically, the ring-pull can is ensured to be in an aseptic state, meanwhile, the can body of the ring-pull can is ensured not to be adversely affected, and the ring-pull can is ensured to be always in the aseptic state by sticking the protective film, so that the edible safety of consumers is ensured.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of better illustrating the invention and is not intended to limit the invention thereto.
Example 1: blue light sterilization and curing of cola and beer cans
The cans were treated as follows:
(1) purchasing two pop cans (36 can of Coca Cola pop can and 36 can of Sandeli beer pop can) from a small retail supermarket in Wuxi city, Jiangsu province, firstly placing 18 cans of Coca Cola pop can and 18 cans of beer pop can at room temperature for 30min, wiping an area 3 cm around a pull ring by using an alcohol cotton swab for degerming, and then inoculating 0.1mL of mixed microbial solution (containing 1 multiplied by 10) into a circular area 2 cm around the pull ring of the pop can6Salmonella strain/mL, 1X 106Staphylococcus aureus per mL, which is a main pollutant detected in the transportation process), placing and volatilizing, and then respectively taking a cola pop can 3 and a beer pop can 3 as a group, and totally dividing into 6 groups at room temperature.
Placing another 18 cans of cola pop can and 18 cans of beer pop can in a refrigerator at 4 deg.C for 30min, wiping with alcohol cotton swab, inoculating the same bacteria solution, standing for volatilizing, and dividing into 6 groups, namely 6 groups in the refrigerator. The room temperature group and the refrigerated group were subjected to the following operations, respectively.
(2) 6 groups at room temperature are taken to respectively carry out blue light irradiation, a 462nm blue light LED lamp is arranged above the pull ring of the pop can at a distance of about 10cm, and the irradiation dose is respectively controlled to be as follows at different times: first group 0J/cm2The second group is 1.25J/cm2And the third group is 2.5J/cm2And the fourth group irradiated at 5J/cm2And the fifth group 10J/cm2And a sixth group of 20J/cm2；
The blue light irradiation operation of the refrigerated 6 groups was the same as that of the room temperature 6 groups, but was performed in a refrigerator during the irradiation process to ensure the temperature maintenance.
(3) After irradiation, a sterile protective film PET film (transparent and with the thickness of 0.25mm) is attached to a circular area 2 cm around the pull ring receiving blue light irradiation, and then irradiation curing is carried out through a blue light photocuring machine (the blue light wavelength is 430 and 485nm, and the irradiation time is 60 seconds), so that the PET film is tightly adhered to the pop can.
After the blue light irradiation, 6 group's samples of room temperature continue to place for 30 days at room temperature, and 6 cold-stored group's samples are torn the pad pasting and are sealed after 4 ℃ freezer storage 30 days, carry out following operation and test:
clamping the sterile cotton ball by using a forceps, and wiping the pull ring of the can opening; then, uniformly coating the substances attached to the sterile cotton balls in a culture medium of a plate culture dish, placing the culture dish in a constant-temperature incubator at 37 ℃, culturing for 24 hours, and then calculating the sterilization rate of the pollution bacteria by referring to GB 4789.2-2010 food microbiology test; the data obtained are the average of triplicates.
The results of the bactericidal ratio test are shown in table 1, and it can be seen from table 1 that: after 462nm blue light irradiation, the second set is 1.25J/cm2And a third group of 2.5J/cm2The sterilization rate is lower than 40%, the sterilization effect is not obvious, and the sterilization efficiency is obviously improved from the fourth group, and the sterilization efficiency is higher than 95%, so the lowest irradiation dose is selected to be 5J/cm2. Therefore, the blue light irradiation and the adhesion of the sterile protective film have obvious synergistic effect, and the sterilization and preservation effects at the pull ring of the pop can beverage can be obviously improved.
TABLE 1 Sterilization efficiency of blue light irradiation on contaminating bacteria at the broke of cola and beer pop can beverages
EXAMPLE 2 wavelength optimization
The fourth group at room temperature (fourth group irradiated at 5J/cm) in example 12) The blue light irradiation wavelength of (2) is adjusted to 400nm, 415nm, 440nm, 460nm, 462nm, 475nm, and 480 nm; the rest and room temperature group 4 of example 1 remained unchanged.
The bactericidal effect is shown in table 2, and it can be seen from table 2 that: the sterilizing efficiency of 462nm, 475nm and 480nm wavelengths is higher than 95%, the sterilizing effect is obvious, while the sterilizing efficiency of 400nm, 415nm, 440nm and 460nm wavelengths is lower than 60%, and the sterilizing effect is lower, so that the 462-480nm range has obvious sterilizing effect on diversified polluted bacteria in the nature.
TABLE 2 comparison of the effectiveness of blue light of different wavelengths in sterilizing contaminating bacteria at the tear-off site of cola and beer cans
Comparative example 1
Two pop cans (3 can coca cola pop can and 3 can Sandeli beer pop can) are purchased from a small retail supermarket in Wuxi city of Jiangsu province, the cola pop can and the beer pop can are placed at room temperature for 30min, an alcohol cotton swab is used for wiping and sterilizing a region 3 cm around a pull ring, and then 0.1mL (containing 1 multiplied by 10) of mixed microbial solution is inoculated into a circular region 2 cm around the pull ring of the pop can6Salmonella strain/mL, 1X 106Staphylococcus aureus per mL) is placed and volatilized, then a 462nm blue-light LED lamp is placed above a pull ring of a room temperature group pop can at a distance of about 10cm, and the time is controlled so that the illumination dose is 5J/cm2And the PET film does not need to be pasted.
Comparative example 2
Two pop cans (3 cans of cola pop cans and 3 cans of beer pop cans) are purchased from a small retail supermarket in Wuxi city, Jiangsu province, the cola pop cans and the beer pop cans are placed at room temperature for 30min, an alcohol cotton swab is used for wiping and sterilizing a region 3 cm around a pull ring, and then 0.1mL (containing 1 multiplied by 10) of mixed microbial solution is inoculated into a circular region 2 cm around the pull ring of the pop cans6Salmonella strain/mL, 1X 106Staphylococcus aureus/mL) is placed and volatilized, and then the protective film-sticking PET film is cured according to the step (3) of the embodiment 1 (the sterile protective film PET film (transparent and 0.25mm in thickness) is stuck to a circular area of 2 cm around the pull ring of the pop can, and then the PET film is tightly adhered to the pop can by irradiation curing through a blue light curing machine (the blue light wavelength is 430-.
The samples of control 1 and control 2 were left for 30 days at room temperature.
The samples of the comparative examples 1 and 2 were tested for their bactericidal effect (same method as in example 1), and the results are shown in table 3, the treatment method of comparative example 1 has very low bactericidal efficiency and is likely to be contaminated by microorganisms in the environment after sterilization, while the treatment method of comparative example 2 has almost no bactericidal effect, whereas example 1 combines the two operations of comparative example 1 and comparative example 2, and combines the sterilization and the maintenance of aseptic conditions to achieve the sterilization and preservation of pop cans.
TABLE 3 comparison of the sterilizing efficiency of different blue light treatment methods on the spoilage site of cola and beer pop-top cans
Comparative example 3
The fourth group at room temperature (fourth group irradiated at 5J/cm) in example 12) The PET film of (1) was modified into a PP film and a PVC film, and the others were kept as in example 1.
The test results are shown in table 4, and it can be seen from table 4 that: the PET material has the best effect, good sealing performance and good food safety.
TABLE 4 comparison of sealing effect of cans of different sealing film materials
Comparative example 4
The fourth group at room temperature (fourth group irradiated at 5J/cm) in example 12) The adhesives of (a) were adjusted to methacrylate adhesives, acrylic pressure sensitive adhesives, camphorquinone, others were kept the same as in example 1.
The test results are shown in table 5, and it can be seen from table 5 that: among 4 common curing agents, the poly (glycerol sebacate) has the best effect and good sealing performance, is a hydrophobic polymer which has good biocompatibility and is biodegradable, and is safe and harmless to human bodies.
TABLE 5 comparison of the sealing effect of cans with different adhesives
Example 3 blue light sterilization and curing of juice and herbal tea Pop-Top cans
Treating the pop can body according to the following method:
(1) 2 canned drinks are purchased from a certain large supermarket in Wuxi city of Jiangsu province, namely canned fruit juice and canned herbal tea (specifically 36 cans of fruit juice and 36 cans of herbal tea pop-top cans), the 18 cans of fruit juice pop-top cans and the 18 cans of herbal tea pop-top cans are placed at room temperature for 30min, an alcohol cotton swab is used for wiping and sterilizing an area 3 cm around a pull ring, then the 3 cans of fruit juice pop-top cans and the 3 cans of herbal tea pop-top cans are taken as a group and are divided into 6 groups at room temperature, and the other part is refrigerated at 4 ℃ and is divided into 6 groups, namely 6 groups. Similar to example 1, 0.1mL (containing 1X 10 of mixed microbial solution) of the mixed microbial solution was inoculated into a circular area 2 cm around the pull tab of the can6Salmonella strain/mL, 1X 106Staphylococcus aureus, 1X 10/mL6Candida albicans per mL), and volatilizing.
Other blue light sterilization and curing bonding operations and analysis methods were the same as in example 1.
As shown in table 6, after the protective film PET film was irradiated and adhered with blue light, the sterilization rates of the second group and the third group were all lower than 50% and the sterilization effect was insignificant, and the sterilization rates of the fourth group, the fifth group and the sixth group were respectively higher than 97% after being placed at room temperature or refrigerated at 4 ℃ (30 days), so that the sterilization effect was very good, and the healthy consumption of consumers was ensured. In addition, the blue light treatment has no influence on the appearance of the easy-open can, compared with the method before irradiation, the method has no obvious difference (p is less than or equal to 0.05), and no unpleasant odor is generated in the irradiation process, so that the health of consumers and the quality of foods in the can are not influenced.
Meanwhile, three blue light treatment modes (sterilization and curing, only sterilization and only curing) are compared, and the following results are found: sterilization and curing are effective ways of sterilizing and ensuring sterility for a long period of time.
In addition, comparison of different sealing film materials and adhesives shows that the PET film has the best effect when matched with the poly (glycerol sebacate), and after being cured by blue light, the blue light sterilized pull ring can keep a sterile state for a long time, so that the edible safety of consumers is ensured.
TABLE 6 Sterilization efficiency of blue light irradiation on contaminated bacteria at the beverage spoiled area of pop cans for fruit juice and herbal tea
Example 4 blue light sterilization and curing of Rice wine and dried pork floss Pop-Top cans
Buying rice wine and pork floss pop-top can, adopting different blue light dose: first group 0J/cm2The second group is 3J/cm2And the third group is 6J/cm2And the fourth group irradiated at 12J/cm2And the fifth group 24J/cm2. Similar to example 1, 0.1mL (containing 1X 10 of mixed microbial solution) of the mixed microbial solution was inoculated in a circular area of 2 cm near the tab of the can61X 10P/mL Pseudomonas aeruginosa6Staphylococcus aureus, 1X 10/mL6Aspergillus flavus spores per ml. These three bacteria are the main contaminants found by analyzing rice wine and dried pork floss pop cans). The other operations were the same as in example 1.
The results are shown in Table 7, and it can be seen from Table 7 that: after blue light irradiation, the second group is 3J/cm2The sterilization rate is lower, and the third group is 6J/cm2The content is increased to 95 percent, the pollution of food-borne pathogenic bacteria can be effectively prevented, and the effect is better.
Similarly, the embodiment also discloses that 462-480nm blue light sterilization is selected, and then the ET film and the poly (glycerol sebacate) are cured and bonded at the pull ring, so that the sterile state at the pull ring can be maintained for a long time, and the health of consumers is facilitated.
TABLE 7 comparison of the sterilizing efficiency of blue light irradiation on rice wine and dried pork floss pop-top can contaminating bacteria
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (5)
1. A blue light disinfection and cleaning method at a pull ring of a pop can is characterized by comprising the following steps:
(1) blue light irradiation is carried out at the pull ring of the pop can; wherein the dose of the blue light irradiation is 5-20J/cm2；
(2) After irradiation is finished, adhering the sterile protective film to the ring pull of the pop can irradiated by the blue light in the step (1) by adopting an adhesive;
the wavelength of the blue light is 462-480 nm;
the sterile protective film is a PET film;
the adhesive is a blue light curing adhesive which adopts blue light waves as an energy source and realizes the curing of a polymerization system through the chemical reaction of excited molecules;
the binder is poly (glycerol sebacate);
the blue light irradiation aiming bacteria are salmonella, staphylococcus aureus, candida albicans, pseudomonas aeruginosa and aspergillus flavus spores.
2. The method as set forth in claim 1, wherein the can is a room temperature or refrigerated can.
3. The method of claim 1, wherein the source of blue light comprises an LED lamp or a fluorescent lamp.
4. The method as claimed in claim 1, wherein the can tab location is a circular area 2 cm around the tab opening.
5. The method as claimed in claim 1, wherein the can is a beverage, beer, herbal tea, coffee, porridge can.
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