CN111955308B - Application of microbial compound bacterium agent in increasing content of total saponins in spina date seeds - Google Patents
Application of microbial compound bacterium agent in increasing content of total saponins in spina date seeds Download PDFInfo
- Publication number
- CN111955308B CN111955308B CN202010896827.0A CN202010896827A CN111955308B CN 111955308 B CN111955308 B CN 111955308B CN 202010896827 A CN202010896827 A CN 202010896827A CN 111955308 B CN111955308 B CN 111955308B
- Authority
- CN
- China
- Prior art keywords
- microbial compound
- added
- sing
- compound bacteria
- spina date
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of spina date seed medicines, and particularly discloses an application of a microbial compound microbial inoculum in improving the content of total saponins in spina date seeds.
Description
Technical Field
The invention relates to the technical field of spina date seed medicines, in particular to application of a microbial compound inoculant in increasing the content of total saponins in spina date seeds.
Background
Wild jujube, a plant of the genus ziziphus of the family Rhamnaceae, is a variety of jujube. Also named as thorn, wild jujube, kudzu, etc., the original product is also distributed in north China and south China. It is usually a shrub or a small arbor in many wild plants. The tree vigor is strong. The shape of branch, leaf and flower is similar to that of common jujube, but the branch has shorter internodes and developed support thorn, and except that each node of the growing branch has support thorn, the support leaf of the bearing branch also becomes sharp support thorn. Small and dense leaves, small, round or oval fruits, thick peel, smooth, purplish red or purple brown, thin flesh, much sour taste, round or oval kernels, smooth surface of kernels, 1 to 2 seeds inside, full kernel and can be used as a traditional Chinese medicine. It has better adaptability than common jujube, long flowering phase, and can be used as honey source plant. Red or purple peel, thin and loose pulp, sour and sweet taste. It has better adaptability than common jujube, long flowering phase, and can be used as honey source plant. Red or purple peel, thin and loose pulp, sour and sweet taste. The spina date seed is a dry mature seed of a Rhamnaceae plant spina date, is produced in northern areas of China, is a traditional natural plant food material with definite effects of nourishing the heart and soothing the nerves in China, is recorded as spina date seed porridge as early as the original generation 'diet essentials', and can treat consumptive disease, vexation and sleeplessness. Spina date seed saponin B, the English name Jujuboside B, molecular formula C52H84O21 molecular weight 1045.21, CAS registry number 55466-05-2.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides application of a microbial compound bacterium agent in improving the content of total saponins in spina date seeds.
In order to achieve the purpose, the invention adopts the following technical scheme:
the application of the microbial compound bacteria in improving the content of the total saponins in the spina date seeds comprises white mushroom (Russula delaica Fr.), eupolyphaga geophilum Fr and Suillus giganteus (Suillus grevillei (Kl.) Sing), and the microbial compound bacteria are added into a soil matrix, then nutrient substances are added, and the spina date seed seedlings are cultured.
Preferably, the soil matrix comprises Chongqing purple soil, Xinjiang lime soil and nutrient soil.
Preferably, the nutrients include sucrose, agar, 6-BA, and IBA.
Preferably, the wild jujube culture environment is as follows: the temperature range is 20-25 ℃, the humidity range is 15% -20%, and the daily sunshine time is 10-14 hours.
Preferably, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum (Suillus grevillei (Kl.) Sing.) are 8: 1:1.
Preferably, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum (Suillus grevillei (Kl.) Sing.) are 1: 8:1.
Preferably, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum (Suillus grevillei (Kl.) Sing.) are 1: 1:8.
Preferably, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum (Suillus grevillei (Kl.) Sing.) are 4: 3:3.
The invention has the beneficial effects that:
the microbial compound microbial inoculum comprises white mushroom (Russula delaca Fr.), euglena terrestris (Cenococcum geophilum Fr.) and boletus caecum (Suillus grevillei (Kl.) Sing), and is added in the process of cultivating wild jujube seedlings, so that the total saponin content of the mature wild jujube kernels can be increased, and the medicinal effect of the wild jujube is improved.
Detailed Description
In order that the invention may be more fully understood, reference will now be made to the following description. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The application of the microbial compound bacteria in improving the content of the total saponins in the spina date seeds comprises white mushroom (Russula delaica Fr.), eupolyphaga geophilum Fr and Suillus giganteus (Suillus grevillei (Kl.) Sing), and the microbial compound bacteria are added into a soil matrix, then nutrient substances are added, and the spina date seed seedlings are cultured.
Preferably, the soil matrix comprises Chongqing purple soil, Xinjiang lime soil and nutrient soil.
Preferably, the nutrients include sucrose, agar, 6-BA, and IBA.
Preferably, the wild jujube culture environment is as follows: the temperature range is 20-25 ℃, the humidity range is 15% -20%, and the daily sunshine time is 10-14 hours.
Example one, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum [ Suillus grevillei (Kl.) Sing ] in the weight percentage of 8: 1:1.
Example two, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum [ Suillus grevillei (Kl.) Sing ] in the weight percentage of 1: 8:1.
Example three, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum [ Suillus grevillei (Kl.) Sing ] in the weight percentage of 1: 1:8.
Example four, the white mushroom (Russula delica Fr.), the soil-borne hollow bolete (Cenococcum geophilum Fr.) and the boletus globosum [ Suillus grevillei (Kl.) Sing ] were 4: 3:3.
In the embodiment, wild jujube is obtained after 180 days of cultivation of wild jujube seedlings, and the content of the total saponins of wild jujube is extracted. Specifically, the wild jujube seed powder is degreased by petroleum ether and then soaked by ethanol, and finally microwave-assisted extraction is carried out twice, and filtration and concentration are carried out to obtain the wild jujube seed total saponin.
Firstly, removing impurities from spina date seeds, and drying the spina date seeds in a constant-temperature blast dryer at 37 ℃; then crushing by a high-speed universal crusher and sieving by a 40-mesh sieve to obtain spina date seed powder; then adding petroleum ether into the spina date seed powder, and performing reflux extraction by using a Soxhlet extractor until the extracting solution is colorless; and then, soaking the wild jujube seed powder treated in the step 2 in 70% ethanol for 30min, wherein the liquid-material ratio is 12-20: 1 ml/g; and finally, performing microwave-assisted extraction twice on the spina date seed powder soaked in the ethanol at the power of 1000W and the temperature of 40-60 ℃ for 4-8 min, filtering and concentrating to obtain the spina date seed total saponins. Through detection, the extraction rate of the spina date seed total saponins is as follows:
the first embodiment is as follows: 3.13 percent;
example two: 3.56 percent;
example three: 3.12 percent;
example four: 4.78 percent;
blank control group: 2.85 percent.
The results show that the microbial compound bacteria with different proportions can improve the content of the total saponins of the spina date seeds, wherein the content of the white mushrooms (Russula delaica Fr.), the soil-born empty-mass bacteria (Cenococcum geophilum Fr.) and the boletus Suillus greville (Kl.) Sing in percentage by weight is 4: the effect of 3: 3 is optimal.
The microbial compound microbial inoculum comprises white mushroom (Russula delaca Fr.), euglena terrestris (Cenococcum geophilum Fr.) and boletus caecum (Suillus grevillei (Kl.) Sing), and is added in the process of cultivating wild jujube seedlings, so that the total saponin content of the mature wild jujube kernels can be increased, and the medicinal effect of the wild jujube is improved.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The application of the microbial compound bacteria in increasing the content of the total saponins in the spina date seeds is characterized in that the microbial compound bacteria comprise white mushroom (Russula deleica Fr.), euglena georginiana (Cenococcum geophilum Fr.) and Suillus pachychniformis (Suillus grevillei (Kl.) Sing), after the microbial compound bacteria are added into a soil matrix, nutrient substances are added, wherein the nutrient substances comprise sucrose, agar, 6-BA and IBA, the zizyphus jujube seedlings are cultured, the white mushroom (Russula delectabica Fr.), the euglena geophilum Fr.) and the suis grifolum (Kl.) Sing are added into the soil matrix in a weight percentage of 4: 3:3.
2. The application of the microbial compound inoculant in increasing the content of total saponins in spina date seeds according to claim 1, wherein the soil matrix comprises Chongqing purple soil and Xinjiang lime soil.
3. The application of the microbial compound inoculant according to claim 1 in increasing the content of total saponins in spina date seeds, wherein the wild jujube culture environment is as follows: the temperature range is 20-25 ℃, the humidity range is 15% -20%, and the daily sunshine time is 10-14 hours.
4. The application of the microbial compound bacteria in increasing the content of the total saponins in the spina date seeds is characterized in that the microbial compound bacteria comprise white mushroom (Russula deleica Fr.), euglena georginiana (Cenococcum geophilum Fr.), and Suillus pachyrhizus (Kl grevillei) Sing, the microbial compound bacteria are added with nutrient substances comprising sucrose, agar, 6-BA and IBA after being added with a soil matrix, wild jujube seedlings are cultured, the white mushroom (Russula deleica Fr.), the euglena georginiana geophilum Fr., and the suis globularis glauca (Kl) Sing are 8 in percentage by weight: 1:1.
5. The application of the microbial compound bacteria in increasing the content of the total saponins of the spina date seeds is characterized in that the microbial compound bacteria comprise white mushroom (Russula deleica Fr.), euglena georginiana (Cenococcum geophilum Fr.) and Suillus pachychniformis (Suillus grevillei (Kl.) Sing), after the microbial compound bacteria are added into a soil matrix, nutrient substances are added, wherein the nutrient substances comprise sucrose, agar, 6-BA and IBA, the zizyphus jujube seedlings are cultured, the white mushroom (Russula delectabica Fr.), the euglena geophilum Fr.) and the suis grifolum (Kl.) Sing are added into the soil matrix according to the weight percentage of 1: 8:1.
6. The application of the microbial compound bacteria in increasing the content of the total saponins of the spina date seeds is characterized in that the microbial compound bacteria comprise white mushroom (Russula deleica Fr.), euglena georginiana (Cenococcum geophilum Fr.) and Suillus pachychniformis (Suillus grevillei (Kl.) Sing), after the microbial compound bacteria are added into a soil matrix, nutrient substances are added, wherein the nutrient substances comprise sucrose, agar, 6-BA and IBA, the zizyphus jujube seedlings are cultured, the white mushroom (Russula delectabica Fr.), the euglena geophilum Fr.) and the suis grifolum (Kl.) Sing are added into the soil matrix according to the weight percentage of 1: 1:8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010896827.0A CN111955308B (en) | 2020-08-31 | 2020-08-31 | Application of microbial compound bacterium agent in increasing content of total saponins in spina date seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010896827.0A CN111955308B (en) | 2020-08-31 | 2020-08-31 | Application of microbial compound bacterium agent in increasing content of total saponins in spina date seeds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111955308A CN111955308A (en) | 2020-11-20 |
| CN111955308B true CN111955308B (en) | 2022-04-12 |
Family
ID=73399505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010896827.0A Active CN111955308B (en) | 2020-08-31 | 2020-08-31 | Application of microbial compound bacterium agent in increasing content of total saponins in spina date seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111955308B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861315A (en) * | 2015-11-22 | 2016-08-17 | 南京金埔园林股份有限公司 | Method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi |
| CN110604048A (en) * | 2018-06-14 | 2019-12-24 | 南京农业大学 | Woody plant mycorrhiza multi-inoculation method and application |
| CN110878258A (en) * | 2019-12-12 | 2020-03-13 | 中国科学院生态环境研究中心 | Growth-promoting compound microbial agent for dahurian larch as well as preparation method and application method thereof |
| CN111072418A (en) * | 2019-12-21 | 2020-04-28 | 赵晓莉 | Compound bacterial fertilizer and preparation method thereof |
| CN111357566A (en) * | 2020-04-10 | 2020-07-03 | 福建农林大学 | Method for quickly cultivating mycorrhizal seedlings of ectomycorrhizal fungi |
-
2020
- 2020-08-31 CN CN202010896827.0A patent/CN111955308B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861315A (en) * | 2015-11-22 | 2016-08-17 | 南京金埔园林股份有限公司 | Method for optimizing nursery-grown plant cultivation by using ectomycorrhizal fungi |
| CN110604048A (en) * | 2018-06-14 | 2019-12-24 | 南京农业大学 | Woody plant mycorrhiza multi-inoculation method and application |
| CN110878258A (en) * | 2019-12-12 | 2020-03-13 | 中国科学院生态环境研究中心 | Growth-promoting compound microbial agent for dahurian larch as well as preparation method and application method thereof |
| CN111072418A (en) * | 2019-12-21 | 2020-04-28 | 赵晓莉 | Compound bacterial fertilizer and preparation method thereof |
| CN111357566A (en) * | 2020-04-10 | 2020-07-03 | 福建农林大学 | Method for quickly cultivating mycorrhizal seedlings of ectomycorrhizal fungi |
Non-Patent Citations (3)
| Title |
|---|
| 不同基质和生物菌肥对南酸枣容器苗生长的影响;罗树凯等;《温带林业研究》;20180615(第02期);第44-47页 * |
| 丛枝菌根对酸枣实生苗耐盐性的影响;申连英等;《土壤学报》;20040630(第03期);第426-433页 * |
| 基质和AM真菌对酸枣幼苗生长及营养的影响;白祯;《中国优秀硕士学位论文全文数据库 农业科技辑》;20131215(第12期);第D048-28 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111955308A (en) | 2020-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1704469A (en) | Method for producing Chinese aweto fungus | |
| CN112359084B (en) | Preparation, prevention and control application of mycoprotein in bacillus subtilis fermentation product | |
| KR20200025135A (en) | Cultivation Method of the Fruiting Bodies of Bio-active Cordyceps sp. Using an Extract of Salvia plebeia | |
| CN111183902B (en) | Tissue culture method for polygonatum sibiricum | |
| CN106119136A (en) | Epicoccum nigrum and application thereof | |
| CN110476709A (en) | A kind of matrix for living body glossy ganoderma dish garden | |
| AU2020101766A4 (en) | Manufacturing method and application of biological fertilizer prepared by kelp residue and bacillus | |
| CN111676168B (en) | Nano-selenium detoxicant and preparation method thereof, detoxified biological protein selenium and preparation method and application thereof | |
| Solouki et al. | Comparison and evaluation of steroid alkaloid solasodine on in vivo and in vitro cultures of Solanum surattense Burm L | |
| CN111955308B (en) | Application of microbial compound bacterium agent in increasing content of total saponins in spina date seeds | |
| CN115851553B (en) | Streptomyces virginiae capable of preventing and treating clubroot and application thereof | |
| CN100362907C (en) | Cremastra appendiculata in vitro culturing and fast reproducing biotechnological method | |
| CN112219638A (en) | Ganoderma lucidum wild-imitating cultivation method | |
| CN110615698A (en) | Preparation method and application of biofertilizer taking enteromorpha and bacillus as raw materials | |
| CN108157183B (en) | Purslane callus induction and suspension culture method | |
| CN109819868B (en) | Dendrobium officinale culture medium and preparation method thereof | |
| CN108432544A (en) | A kind of cultural method of ramulus mori cloud ear | |
| CN102356908A (en) | Herba violae beverage and preparation method thereof | |
| KR20210035601A (en) | Production method for cultured root of leguminous plants comprising high-content of coumestrol using methyl jasmonate treatment | |
| CN110655424A (en) | Preparation method and application of biofertilizer taking enteromorpha and burkholderia pyrrocinia as raw materials | |
| KR100464704B1 (en) | Manufacturing method of fermented alcoholic drink using medicinal and eatable plants having organic germanium and fermented alcoholic drink obtained therefrom | |
| CN111955483A (en) | Application of microbial compound bacterium agent in increasing content of spina date seed spinosad | |
| CN105713845B (en) | A method of cigarette Yunnan olive fragrance is prepared using trichoderma aureoviride fermentation | |
| CN110183273A (en) | A kind of fertilizer and its preparation process of the growth substance containing natural plants | |
| CN110218109A (en) | A kind of high-quality Cranberry growth regulator and preparation method thereof and application method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |