CN111944718A - Agricultural microbial comprehensive preparation - Google Patents
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Abstract
The invention provides an agricultural microbial comprehensive preparation, which belongs to the technical field of microbial preparations and is prepared from the following raw materials in parts by weight: 1-97% of A preparation, 1-97% of B preparation, 1-97% of C1 preparation and 1-97% of C2 preparation; the preparation has the advantages of obtaining the effect that the quality and the yield of agricultural products are higher than those of the planting effect of chemical fertilizers and pesticides by being lower than or equal to the input of the chemical fertilizers and the pesticides, realizing no residue, resistance and pollution, and providing the possibility of practical application for replacing the chemical fertilizers and the pesticides.
Description
Technical Field
The invention relates to the technical field of microbial preparations, in particular to an agricultural microbial comprehensive preparation.
Background
In recent years, the destruction of fertilizers and pesticides (including chemical herbicides and chemical growth hormones, the same applies hereinafter) to the ecological environment is more and more serious, and the great harm of pollutants and residues in agricultural products to the health of human beings is more and more accepted. However, chemical fertilizers and pesticides are still main production data for increasing both production and income of agriculture at present, and bring great benefits to human beings and also bring great threat to human environment and health. What techniques and products can replace fertilizers and pesticides with the same method, that is, the same yield can be obtained with the same investment, without damaging the ecological environment and affecting the survival and health of human beings? This is a major issue that people need to solve urgently and is a serious challenge to the progress of modern science and technology and human civilization.
Organic fertilizers (including farmyard manure) can replace fertilizers. In the farming era of thousands of years, no chemical fertilizer is available, but the dosage, the fund and the labor input of the organic fertilizer are several times of those of the chemical fertilizer to achieve the yield of the chemical fertilizer. Human beings have been unable to return to the rough times of knife cultivation and fire planting, and traditional farming methods without chemical fertilizers and pesticides are not viable nowadays.
Organic planting does not use chemical fertilizers and pesticides. However, the current organic planting and chemical fertilizer and pesticide planting are not equal in investment, the organic planting investment is high, the yield is low, the wide requirements cannot be met, and the chemical fertilizer and pesticide cannot be replaced.
The tolerance of crops and soil to chemical fertilizers makes the fertilizer efficiency worse and worse the more chemical fertilizers are applied. Chemical fertilizers remain in soil to cause hardening and hardening of cultivated land, remain in agricultural products to damage human health, and run off in a water system to cause extensive pollution of non-point sources. Therefore, under the condition that the prior art still can not replace chemical fertilizers and pesticides, methods for saving the chemical fertilizers and the pesticides such as soil testing formula fertilization, scientific fertilization, zero growth of the chemical fertilizers and the pesticides and the like are provided, so that the method is not durable and can not solve the fundamental problem.
The microbial fertilizer or the biological organic fertilizer can replace a chemical fertilizer. The development of preferential policies such as tax free are given by the country to encourage the development for many years, but the preferential policies are still used as an aid of the fertilizer, and the situation of replacing the fertilizer cannot be formed for many years. Of course, the application of the fertilizer is expensive, and as in organic planting, the application is not equal to the application of chemical fertilizer and pesticide (high application and low output), so that the wide demand cannot be met.
Microbial pesticides and biopesticides are alternatives to chemical pesticides. But why has not hitherto been a situation where chemical pesticides have been widely used and discarded? Mainly caused by factors such as high price, single strain or function, difficult preservation, unstable effect and the like.
The resistance of viruses and pests to chemical pesticides is also increasing, with more pesticides and varieties being used. The chemical pesticide has no selectivity to biological killing, can not be sprayed in a large area, is used locally to form driving, and cannot kill thoroughly. When the chemical pesticide is used, natural enemies of pests are killed, beneficial microbial bacteria in soil are inactivated, the natural ecological environment required by the natural growth of crops is destroyed, and the chemical pesticide comprises a large amount of residues of chemical herbicides and chemical growth hormones, so that the human health is seriously threatened. However, there is still no method for effectively solving these problems.
Disclosure of Invention
Aiming at the technical defects, the invention aims to provide an agricultural microorganism comprehensive preparation which has the advantages that the quality and the yield of agricultural products are higher than the effect of planting fertilizer pesticides by using the preparation which is lower than or equal to the input of the fertilizer pesticides, no residue, no resistance and no pollution are realized, and the possibility of practical application is provided for replacing the fertilizer pesticides.
In order to solve the technical problems, the invention adopts the following technical scheme:
an agricultural microbial comprehensive preparation is characterized by being prepared from the following raw materials in parts by weight:
1-97% of A preparation, 1-97% of B preparation, 1-97% of C1 preparation and 1-97% of C2 preparation;
the preparation A comprises rhizobia, azospirillum, bacillus megaterium, bacillus mucilaginosus and an adsorbing material;
the preparation B comprises Bacillus thuringiensis Israeli subspecies, Trichoderma harzianum, Streptomyces microflavus, Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea, Paecilomyces lilacinus, Trichoderma viride and an adsorbing material;
the C1 preparation comprises bacillus subtilis, bacillus licheniformis, bacillus pumilus, trichoderma pseudokoningii and an adsorbing material;
the C2 preparation comprises Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbrueckii and adsorbing material.
Preferably, the rhizobium culture medium formula consists of the following raw materials in percentage by weight: 1.0 percent of molasses, 0.05 percent of dipotassium phosphate, 0.3 percent of calcium carbonate, 0.02 percent of magnesium sulfate, 0.04 percent of yeast extract, 0.0002 percent of sodium molybdate and sterile water for supplementing 100 percent;
the formula of the nitrogen-fixing spirochete culture medium is composed of the following raw materials in parts by weight: 1.0 percent of molasses, 0.01 percent of dipotassium hydrogen phosphate, 0.04 percent of monopotassium phosphate, 0.02 percent of magnesium sulfate, 0.01 percent of sodium chloride, 0.002 percent of calcium chloride, 0.002 percent of ferrous sulfate, 0.0002 percent of sodium molybdate and sterile water which is up to 100 percent;
the bacillus megaterium culture medium formula consists of the following raw materials in percentage by weight: 1.0 percent of corn starch, 0.6 percent of molasses, 1.5 percent of beef extract, 0.1 percent of ammonium sulfate, 0.2 percent of dipotassium hydrogen phosphate, 0.1 percent of calcium carbonate, 0.05 percent of magnesium sulfate, 0.02 percent of zinc sulfate and the balance of sterile water to 100 percent;
the bacillus mucilaginosus culture medium formula consists of the following raw materials in percentage by weight: 2% of molasses, 0.5% of soybean meal powder, 0.1% of magnesium sulfate, 0.05% of dipotassium phosphate, 0.1% of calcium carbonate and the balance of sterile water to 100%.
Preferably, the formulation of the culture medium for bacillus thuringiensis israelensis comprises the following raw materials in parts by weight: 2.0 percent of molasses, 0.5 percent of peptone, 0.5 percent of yeast extract, 0.03 percent of dipotassium phosphate, 0.1 percent of disodium phosphate, 0.1 percent of magnesium sulfate, 0.002 percent of ferrous sulfate, 0.002 percent of calcium chloride and the balance of sterile water to 100 percent;
the formula of the trichoderma harzianum culture medium comprises the following raw materials in parts by weight: 20% of potato cooking juice, 0.3% of dipotassium phosphate, 2.0% of sugar, 0.15% of magnesium sulfate, 0.1% of vitamin and sterile water for supplementing 100%;
the culture medium formula of the streptomyces microflavus comprises the following raw materials in parts by weight: 2.0% of soluble starch, 0.1% of potassium nitrate, 0.05% of dipotassium phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate, 0.05% of sodium chloride and sterile water for supplementing 100%;
the formula of the beauveria bassiana culture medium comprises the following raw materials in parts by weight: 5.0 percent of molasses, 0.1 percent of dipotassium phosphate, 1 percent of corn leaching liquor and sterile water which are complemented to 100 percent;
the formula of the metarhizium anisopliae culture medium comprises the following raw materials in parts by weight: 4.0 percent of molasses, 1.0 percent of peptone, 1.0 percent of yeast extract, 0.15 percent of dipotassium phosphate, 0.05 percent of magnesium sulfate, 0.03 percent of sodium borate, 0.04 percent of manganese sulfate, 0.05 percent of sodium molybdate, 0.08 percent of copper sulfate, 0.4 percent of zinc sulfate, 0.01 percent of ferrous sulfate, trace vitamin B1 and sterile water which is up to 100 percent;
the isaria fumosorosea culture medium formula consists of the following raw materials in percentage by weight: molasses 2.0%, dipotassium hydrogen phosphate 0.3%, magnesium sulfate 0.15%, and 20% of potato extract to make up 100%.
The formula of the lilac paecilomyces culture medium comprises the following raw materials in parts by weight: 1.5 percent of molasses, 0.5-1.0 percent of soluble starch, 0.5 percent of ammonium sulfate, 0.1 percent of dipotassium phosphate, 0.01 percent of zinc sulfate, 0.025 percent of calcium carbonate, 0.001 percent of manganese sulfate and the balance of sterile water to 100 percent;
the formula of the trichoderma viride culture medium comprises the following raw materials in parts by weight: 1.0 percent of molasses, 1.3 percent of corn flour, 0.5 percent of soybean flour, 0.3 percent of dipotassium phosphate, 0.2 percent of ammonium nitrate and sterile water which make up to 100 percent.
Preferably, the bacillus subtilis culture medium formula consists of the following raw materials in percentage by weight: 1.5 percent of molasses, 0.05 percent of dipotassium phosphate, 0.05 percent of yeast extract, 0.02 percent of beef extract, 0.05 percent of peptone, 0.025 percent of sodium chloride, 0.001 percent of manganese sulfate and the balance of sterile water to 100 percent;
the culture medium formula of the bacillus licheniformis is composed of the following raw materials in parts by weight: 1.5 percent of molasses, 1.0 percent of yeast extract, 0.3 percent of magnesium sulfate, 0.1 percent of beef extract, 0.05 percent of peptone, 0.025 percent of calcium carbonate, 0.02 percent of sodium chloride and the balance of sterile water to 100 percent;
the bacillus pumilus culture medium formula comprises the following raw materials in parts by weight: molasses 0.5%, corn flour 0.5%, peptone 1.5%, bean cake powder 0.5%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.2%, and sterile water to make up 100%;
the trichoderma pseudokoningii culture medium formula consists of the following raw materials in percentage by weight: molasses 0.75%, wheat bran 3.0%, dipotassium hydrogen phosphate 0.1%, corn flour 3.0%, and sterile water to make up 100%.
Preferably, the lactobacillus bulgaricus culture medium formula consists of the following raw materials in grams by weight: 20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L;
the lactobacillus acidophilus culture medium formula consists of the following raw materials in parts by weight: 20g of sucrose, 20g of peptone, 1g of sodium chloride, 0.01g of manganese sulfate, 0.02g of magnesium sulfate, 0.5g of sodium acetate and the balance of sterile water to 1L;
the lactobacillus plantarum culture medium formula consists of the following raw materials in parts by weight: 20g of sucrose, 25g of yeast extract, 2g of dipotassium phosphate, 10g of casein peptone, 10g of beef extract, 5g of sodium acetate, 2g of diamine citrate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 801 g of tween and 1L of sterile water;
the lactobacillus delbrueckii culture medium formula consists of the following raw materials in weight gram: 20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L.
Preferably, the adsorbing material is prepared from the following raw materials in percentage by weight: 50-97% of calcined vermiculite powder, 1-48% of humic acid, 1-48% of turfy soil and 1-48% of attapulgite.
The invention has the beneficial effects that: the preparation has the advantages of obtaining the effect that the quality and the yield of agricultural products are higher than those of the planting effect of chemical fertilizers and pesticides by being lower than or equal to the input of the chemical fertilizers and the pesticides, realizing no residue, resistance and pollution, and providing the possibility of practical application for replacing the chemical fertilizers and the pesticides.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
An agricultural microbial comprehensive preparation is prepared from the following raw materials in parts by weight:
1-97% of A preparation, 1-97% of B preparation, 1-97% of C1 preparation and 1-97% of C2 preparation;
the preparation A comprises rhizobium, azospirillum, bacillus megaterium, bacillus mucilaginosus and an adsorbing material;
the preparation B comprises Bacillus thuringiensis Israeli subspecies, Trichoderma harzianum, Streptomyces microflavus, Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea, Paecilomyces lilacinus, Trichoderma viride and an adsorbing material;
the C1 preparation comprises Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Trichoderma pseudokoningii, and adsorbent material;
the C2 preparation comprises Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbrueckii and adsorbent material.
The technical scheme of the invention is as follows:
4 bacteria agents for promoting plant growth are combined in proportion to prepare A preparation, 8 biocontrol bacteria agents for preventing and treating plant diseases and insect pests are combined in proportion to prepare B preparation, 4 aerobic decomposition bacteria agents are combined in proportion to prepare C1 preparation, and 4 anaerobic decomposition bacteria are combined in proportion to prepare C2 preparation. Then, mixing A + B + C1+ C2 in proportion to prepare the comprehensive preparation.
The scheme adopts a liquid microbial inoculum propagation technology for respectively carrying out open fermentation on aerobic bacteria and closed fermentation on anaerobic bacteria, so that the production propagation of the microbial inoculum realizes low cost and high effect; the mixed adsorbent containing a plurality of beneficial elements is adopted, so that the adsorption capacity of the microbial inoculum is increased, the activity is lasting, and medium and trace elements are added; the technology of mixing aerobic bacteria and anaerobic bacteria is adopted, so that conditions are created for the complementary symbiosis of the two bacteria and the activity maintenance; the method of secondary propagation before use is adopted, so that the cost is reduced again, and the effectiveness of the function of the microbial inoculum is ensured; the method of adjusting the use ratio of the three preparations in the comprehensive preparation according to the actual condition is more practical and targeted.
The important characteristics of the comprehensive preparation are as follows: promoting the growth of plants and fully utilizing organic fertilizer; the pesticide can prevent and treat various plant diseases and insect pests, does not generate resistance, does not hurt natural enemies of the pests, and does not harm the health of users; biologically decomposing the residual pollutants in soil and water, including antibiotics, aromatics, chemical pesticides, chemical fertilizers, growth hormones, heavy metals and the like; the hardened, acidified and salinized soil is improved, and the soil aggregate structure and the benign micro-ecological environment are recovered; the fertilizer and pesticide are replaced by the input which is lower than or equal to the input of the fertilizer and pesticide, the quality and the yield of agricultural products are higher than those of the fertilizer and pesticide planting, and no residue is left
Preparation method and steps of A preparation
The preparation A consists of the following 4 aerobic bacteria agents:
1. rhizobia, latin scientific name Rhizobium, bacterial kingdom, main functional role: nitrogen fixation is carried out by symbiosis with leguminous plants, and molecular nitrogen naturally existing in the air is reduced into ammonia nitrogen, so as to provide nitrogen nutrition for the plants.
2. Azospirillum, bacterial kingdom, latin school name: azospirillum, main functional role: promoting the combined nitrogen fixation of leguminous crops and non-leguminous crops.
3. Bacillus megatherium, bacteria kingdom, latin university name: bacillus megaterium, the main functional role: dissolving phosphorus, decomposing inorganic phosphorus and organic phosphorus, degrading organic phosphorus pesticides and aflatoxin, and improving soil fertility.
4. Bacillus mucilaginosus, bacterial kingdom, latin school name: bacillus mucoginosus, main functional role: the potassium and the phosphorus are dissolved, the nitrogen is fixed, the solidified medium and trace elements such as calcium, magnesium and sulfur in the soil are released, the stress resistance of crops is enhanced, the quality is improved, and the yield is increased.
Firstly, preparing liquid A microbial inoculum
1. According to the formula and the culture conditions, 4 strains are respectively inoculated into a triangular cup, and the triangular cup is put on a shaking table for seed culture.
2. According to the formula and culture conditions, respectively inoculating 4 liquid inocula into a fermentation tank, and carrying out primary amplification culture of the inocula.
3. And repeating the step 2 to perform secondary and tertiary amplification culture of the microbial inoculum. 4 liquid A bactericides after three-stage culture are obtained.
A propagation method of a liquid aerobic bacteria preparation.
Aerobic bacteria in the preparation are respectively propagated by a liquid microbial inoculum of open aerobic fermentation, so that dominant flora can reject and phagocytize infectious microbes. The strain cultured and propagated by the method has high activity, strong tolerance and low cost. The specific method comprises the following steps: injecting natural water (well water, river water, lake water) into the tank, introducing ozone for 30min, sterilizing, standing for 24 hr, injecting culture medium, mixing, adding strain, stirring and propagating according to required conditions without sealing the tank opening.
Culture medium formula and culture conditions of liquid A microbial inoculum
1. The rhizobium culture medium formula comprises:
1.0 percent of molasses, 0.05 percent of dipotassium phosphate, 0.3 percent of calcium carbonate, 0.02 percent of magnesium sulfate, 0.04 percent of yeast extract, 0.0002 percent of sodium molybdate and sterile water for supplementing 100 percent.
Fermentation conditions are as follows: the inoculation amount is 4 percent, the initial pH value is 7.1-7.5, the rotation speed is 110-.
2. The formula of the nitrogen-fixing spirochete culture medium comprises the following components:
1.0 percent of molasses, 0.01 percent of dipotassium hydrogen phosphate, 0.04 percent of monopotassium phosphate, 0.02 percent of magnesium sulfate, 0.01 percent of sodium chloride, 0.002 percent of calcium chloride, 0.002 percent of ferrous sulfate and sodium molybdate. 0.0002% and sterile water to make up 100%.
Fermentation conditions are as follows: the inoculation amount is 4 percent, the initial pH value is 7.2-7.4, the rotation speed is 110-.
3. The formula of the bacillus megaterium culture medium comprises the following components:
1.0 percent of corn starch, 0.6 percent of molasses, 1.5 percent of beef extract, 0.1 percent of ammonium sulfate, 0.2 percent of dipotassium hydrogen phosphate, 0.1 percent of calcium carbonate, 0.05 percent of magnesium sulfate, 0.02 percent of zinc sulfate and sterile water which make up 100 percent.
Fermentation conditions are as follows: the inoculation amount is 4 percent, the initial pH value is 7.1-7.5, the rotation speed is 110-.
4. The formula of the bacillus mucilaginosus culture medium comprises the following components:
2% of molasses, 0.5% of soybean meal powder, 0.1% of magnesium sulfate, 0.05% of dipotassium phosphate, 0.1% of calcium carbonate and the balance of sterile water to 100%.
Fermentation conditions are as follows: the inoculation amount is 4 percent, the initial pH value is 7.1-7.5, the rotation speed is 110-.
Second, preparing solid A preparation
1. Respectively mixing 4 liquid A bactericides according to the proportion of 2 portions of the bactericides and 1 portion of adsorbing materials to prepare 4A preparations of different strains.
2. 4A preparations are prepared according to the actual planting requirements. The preparation proportion is as follows: 1-97% of rhizobium preparation, 1-97% of azospirillum preparation, 1-97% of bacillus megaterium preparation and 1-97% of bacillus mucilaginosus preparation.
Preparation method and steps of B preparation
The preparation B consists of 8 biocontrol microbial agents of the following bacteria;
1. bacillus thuringiensis subspecies israelensis, Latin scientific name Bacillus thuringiensis israelensis Bti, bacterial kingdom, main functional role: is a safe bacterial pesticide which is friendly to human and environment, has toxic and killing effects on more than 200 lepidoptera pests, and can be widely spread in pest affected areas.
2. Trichoderma harzianum, kingdom fungoides, name of latin: trichoderma harzianun. The main functions are as follows: preventing and treating various soil-borne diseases, including powdery mildew, gray mold, rust disease, downy mildew, fusarium wilt, verticillium wilt, rear spot disease, anthracnose, root rot and the like; improve the granular structure of soil, strengthen the disease resistance of plants and increase the crop yield.
3. Streptomyces microflavus, kingdom bacteria, title latin: streptomyces microflavus, major functional role: the soil nitrogen phosphorus potassium nutrient components are converted, the soil fertility is improved, the germ reproduction is inhibited, the disease prevention and seedling protection are realized, the rooting and the sprouting of crops are promoted, and the yield is increased. The quality of agricultural products is improved.
4. Beauveria bassiana, Latin name: beauveria gossiana, kingdom fungoides, aerobic bacteria, main functional role: the insecticidal fungus is widely used for controlling pests, and has an infection killing effect on 707 pests of 15 orders, 149 families, 521 genera and 707 varieties such as lepidoptera, coleoptera and the like.
5. Metarhizium anisopliae, Latin's name Metarhizium anisopliae, fungus, aerobic bacteria; the main functions are as follows: broad spectrum insecticidal fungus, and more than two hundred kinds of insects can be killed by infection.
6. Isaria Fumosorosea, Latin's name Fumosososea wize, entomopathogenic fungi, aerobic bacteria, main functional role: can infect and kill various pests of hemiptera, coleopteran, dipteran, homopteran, etc.
7. Lilac paecilomyces, Latin scientific name: paecilmyces lilacinus samson. Endoparasitic fungi, aerobic bacteria. The main functions are as follows: the parasitic insects kill various nematodes and stimulate the growth of crops.
8. Trichoderma viride, fungi kingdom and aerobic bacteria. Latin learning name: trichoderma Viride, main functional role: produce enzyme, decompose fiber, and effectively prevent and treat soil-borne diseases.
Firstly, preparing liquid B microbial inoculum
1. According to the formula and the culture conditions, respectively inoculating 8 strains into a triangular cup, and performing seed culture on a shaking table.
2. According to the formula and the culture strip, respectively inoculating 8 liquid inocula into a fermentation tank, and carrying out primary amplification culture of the inocula.
3. And repeating the step 2 to perform secondary and tertiary amplification culture of the microbial inoculum. Respectively obtaining 8 liquid B bactericides after three-stage culture.
Culture medium formula and culture conditions of liquid B microbial inoculum
1. The formula of the bacillus thuringiensis culture medium comprises the following components:
2.0 percent of molasses, 0.5 percent of peptone, 0.5 percent of yeast extract, 0.03 percent of dipotassium phosphate, 0.1 percent of disodium phosphate, 0.1 percent of magnesium sulfate, 0.002 percent of ferrous sulfate, 0.002 percent of calcium chloride and the balance of sterile water of 1L;
the culture conditions are as follows: the initial pH value is 7.2, the temperature is 25-35 ℃, the rotation speed is 120-;
2. the formula of the trichoderma harzianum culture medium comprises the following components:
comprehensive potato culture medium:
2.0 percent of sugar, 0.3 percent of dipotassium phosphate, 0.15 percent of magnesium sulfate, 0.1 percent of vitamin and 20 percent of potato juice to make up 100 percent.
The preparation method of the 20% potato juice comprises the following steps: taking peeled potato 200g, cutting into small pieces, adding water, boiling for 30min, and adding sterile water to 1000 ml.
Fermentation conditions are as follows: initial Ph is 7.2-7.4, 28-35 ℃, the rotation speed is 120-.
3. The formula of the streptomyces microflavus culture medium comprises the following components:
2.0% of soluble starch, 0.1% of potassium nitrate, 0.05% of dipotassium phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate, 0.05% of sodium chloride and sterile water for supplementing 100%;
the culture conditions are as follows: the initial pH value is 7.2, the temperature is 25-35 ℃, the rotation speed is 120-;
4. the formula of the beauveria bassiana culture medium comprises the following components:
5.0 percent of molasses, 0.1 percent of dipotassium phosphate, 1 percent of corn leaching liquor and sterile water which are complemented to 100 percent;
the culture conditions are as follows: the initial pH value is 7.0, the light is shielded, the temperature is 25-37 ℃, the temperature is 120-;
5. the formula of the metarhizium anisopliae culture medium comprises the following components:
4.0 percent of molasses, 1.0 percent of peptone, 1.0 percent of yeast extract, 0.15 percent of dipotassium phosphate, 0.05 percent of magnesium sulfate, 0.03 percent of sodium borate, 0.04 percent of manganese sulfate, 0.05 percent of sodium molybdate, 0.08 percent of copper sulfate, 0.4 percent of zinc sulfate, 0.01 percent of ferrous sulfate, trace vitamin B1 and sterile water which are up to 100 percent.
The culture conditions are as follows: 30-37 ℃, initial ph of 6.5-7.2, and rotation number of 120-140r/min, ventilating, shaking, culturing and fermenting for 72-96 hours.
6. The formula of the Isaria fumosorosea culture medium comprises the following components:
2.0% of molasses, 0.3% of dipotassium phosphate, 0.15% of magnesium sulfate and 20% of potato extract to make up 100%;
the culture conditions are as follows: initial pH value is 7.0, shading is carried out at 25-37 ℃, temperature is 120-.
7. The formula of the culture medium of the lilac paecilomyces comprises the following components:
1.5 percent of molasses, 0.5-1.0 percent of soluble starch, 0.5 percent of ammonium sulfate, 0.1 percent of dipotassium phosphate, 0.01 percent of zinc sulfate, 0.025 percent of calcium carbonate, 0.001 percent of manganese sulfate and the balance of sterile water to 100 percent.
The culture conditions are as follows: 28-30 ℃, initial ph of 6-7, and aerobic shake culture for 60-120h at 180 r/min.
8. The formula of the trichoderma viride culture medium comprises the following components:
1.0 percent of molasses, 1.3 percent of corn flour, 0.5 percent of soybean flour, 0.3 percent of dipotassium phosphate, 0.2 percent of ammonium nitrate and sterile water which make up to 100 percent.
The culture conditions are as follows: 28-30 ℃, initial ph of 6-7, and aerobic shake culture for 60-120h at 180 r/min.
Further, the 1% corn extract is prepared by adding water 1L into 10g of fresh corn kernels, boiling for 30 minutes, filtering to remove residues, and supplementing sterile water to 1L; the 20% potato extractive solution is prepared from peeled potato 200g, cutting into small pieces, adding water 1L, boiling for 30min, filtering to remove residue, and supplementing sterile water to 1L.
Second, preparing solid B preparation
1. Respectively mixing 8 liquid B bactericides according to the proportion of 2 portions of the bactericides and 1 portion of the adsorbing materials to prepare 8B preparations of different strains.
2. 8 preparations were formulated. The prepared proportion can be adjusted according to the actual planting need: the Bacillus thuringiensis Israeli subspecies preparation accounts for 1-93%, the Trichoderma harzianum preparation accounts for 1-93%, the Streptomyces lavendulae preparation accounts for 1-93%, the Beauveria bassiana preparation accounts for 1-93%, the Metarhizium anisopliae preparation accounts for 1-93%, the Isaria fumosoroseus preparation accounts for 1-93%, the Paecilomyces lilacinus preparation accounts for 1-93%, and the Trichoderma viride preparation accounts for 1-93%.
Preparation method and steps of C1 preparation
The C1 preparation is prepared from the following microbial inoculum
1. Bacillus subtilis, Latin's name Bacillus subtilis, bacterium, prokaryote kingdom, aerobic facultative anaerobe. The main functions are as follows: inhibiting pathogenic bacteria, inducing plant to generate disease resistance, degrading agricultural chemical and heavy metal residue, purifying and improving soil.
2. Bacillus licheniformis, bacterial kingdom, aerobic bacteria, Latin science name Bacillus licheniformis, the main functional role: inhibiting pathogenic bacteria, improving disease resistance of crops, degrading agricultural chemical residue, and improving, purifying and repairing soil.
3. Bacillus pumilus, bacterium kingdom, aerobic bacteria, Latin's name; bacillus pumilus. The main functions are as follows: improving the disease resistance, cold resistance and drought resistance of crops, increasing soil nutrients, improving soil structure and promoting the decomposition of organic matters and the growth of crops. Fixing nitrogen, dissolving phosphorus and dissolving potassium.
4. Trichoderma pseudokoningii, fungi kingdom and aerobic bacteria. The Latin scientific name Trichoderma pseudokiningii. The main functions are as follows: decomposing fiber, and biologically preventing and treating gray botrytis, gray mold, fusarium oxysporum, leaf mold, early blight, anthracnose, blight, etc.
Firstly, preparing liquid C1 microbial inoculum
1. According to the formula and the culture conditions, 4 strains are respectively inoculated into a triangular cup, and the triangular cup is put on a shaking table for seed culture.
2. According to the formula and the culture strip, respectively inoculating 4 liquid inocula into a fermentation tank, and carrying out primary amplification culture of the inocula.
3. And repeating the step 2 to perform secondary and tertiary amplification culture of the microbial inoculum. 4 liquid C1 microbial inoculum which is subjected to three-stage culture is obtained.
Culture medium formula and culture conditions of liquid C1 microbial inoculum
1. The liquid culture medium formula of the bacillus subtilis comprises the following components:
1.5 percent of molasses, 0.05 percent of dipotassium phosphate, 0.05 percent of yeast extract, 0.02 percent of beef extract, 0.05 percent of peptone, 0.025 percent, 0.02 percent of sodium chloride, 0.001 percent of manganese sulfate and sterile water which is up to 100 percent.
The culture conditions are as follows: 28-30 ℃, initial ph 6-7, and 120-180r/min, and aerobic shake culture for 48-36 h.
2. The liquid culture medium formula of the bacillus licheniformis comprises the following components: 1.5 percent of molasses, 1.0 percent of yeast extract, 0.3 percent of magnesium sulfate, 0.1 percent of beef extract, 0.05 percent of peptone, 0.025 percent of calcium carbonate, 0.02 percent of sodium chloride and the balance of sterile water to 100 percent.
The culture conditions are as follows: 28-30 ℃, initial ph 6-7, and 120-180r/min, and aerobic shake culture for 48-36 h.
3. The liquid culture medium formula of the bacillus pumilus comprises the following components: molasses 0.5%, corn flour 0.5%, peptone 1.5%, bean cake powder 0.5%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.2%, and sterile water to make up 100%.
The culture conditions are as follows: 28-30 ℃, initial ph 6-7, 200r/min, aerobic shake culture for 48-36 h. .
4. The formula of the liquid culture medium of trichoderma pseudokoningii comprises the following components: molasses 0.75%, wheat bran 3.0%, dipotassium hydrogen phosphate 0.1%, corn flour 3.0%, and sterile water to make up 100%.
The culture conditions are as follows: 28-30 ℃, initial ph of 5.0-6.0, 120-.
Second, solid C1 preparation is prepared
1. Respectively mixing 4 liquid C1 microbial inoculum according to the proportion of 2 parts of microbial inoculum and 1 part of adsorption material to respectively prepare C1 preparations of 4 different strains.
2. 4C 1 preparations are prepared according to the actual planting requirements. The preparation proportion is as follows: 1-97% of bacillus subtilis preparation, 1-97% of bacillus licheniformis preparation, 1-97% of bacillus pumilus preparation and 1-97% of trichoderma pseudokoningii preparation.
Method for preparing C2 preparation and its steps
The C2 preparation consisted of the following anaerobic inoculants:
1. lactobacillus bulgaricus, the latin scientific name Lactobacillus bulgaricus, bacteria, anaerobes, the main functional functions are as follows: acid production, namely digesting and converting complex organic matters and colloidal insoluble harmful organic matters into organic acids and alcohols; produce gas and oxygen, and provide excellent conditions for the mutual benefit and symbiosis with aerobic bacteria.
2. Lactobacillus acidophilus, the latin scientific name Lactobacillus acidophilus, bacteria, anaerobes; the main functions are the same as those in 1.
3. Lactobacillus plantarum, Latin scientific name Lactobacillus plantarum, bacterium, anaerobe, the main functional role (same as 1).
4. Lactobacillus delbrueckii subsp, bacterial, anaerobic; the main functions are the same as those of 1.
Firstly, preparing liquid C2 microbial inoculum
1. Respectively preparing liquid anaerobic bacteria culture media of lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus plantarum and lactobacillus delbrueckii (see 'culture medium formula and culture conditions of 4 liquid C2 microbial inocula'), respectively inoculating respective corresponding strains into the 4 liquid anaerobic bacteria culture media, standing and sealing for fermentation for 48-72 hours at the temperature of 25-37 ℃, and performing first-stage expanded culture of the strains.
2. The 4 kinds of anaerobic bacteria liquid of the first-stage culture are prepared and mixed together according to the following proportion, and the mixture is sealed and kept stand for 24 hours, so that the anaerobic mixed bacteria liquid of the first-stage fermentation is obtained. The preparation proportion is as follows: 1-97% of lactobacillus bulgaricus preparation, 1-97% of lactobacillus acidophilus preparation, 1-97% of lactobacillus plantarum preparation and 1-97% of lactobacillus delbrueckii preparation.
3. Preparing a mixed fermentation liquid anaerobic bacteria culture medium (see a culture medium formula and culture conditions of 4 liquid C2 microbial inoculum), inoculating the anaerobic mixed bacterial liquid subjected to primary fermentation into the mixed fermentation liquid anaerobic bacteria culture medium, wherein the inoculation amount is 5-10% of the mass of the culture medium, standing the mixed fermentation liquid anaerobic bacteria culture medium at the temperature of 30-37 ℃ for sealed anaerobic fermentation for 48-72 hours, and performing secondary amplification culture on the strains to obtain a mixed fermentation secondary anaerobic bacterial liquid;
4. and repeating the step 3 to carry out three-stage amplification culture of the microbial inoculum. A mixed anaerobically fermented liquid C2 preparation was obtained.
Second, solid C2 preparation is prepared
And mixing the mixed anaerobic fermentation liquid C2 preparation into a C2 preparation according to the proportion of 2 parts of microbial inoculum and 1 part of adsorption material.
Culture medium formula and culture conditions of 4 liquid C2 microbial inoculum
1. Lactobacillus bulgaricus, culture medium formula and culture conditions:
20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L; the culture conditions are as follows: initial pH value of 6.0, 30-37 deg.C, shading, sealing, standing for anaerobic culture for 48-72 hr;
2. lactobacillus acidophilus, culture medium formula and culture conditions:
20g of sucrose, 20g of peptone, 1g of sodium chloride, 0.01g of manganese sulfate, 0.02g of magnesium sulfate, 0.5g of sodium acetate and the balance of sterile water to 1L; the culture conditions are as follows: initial pH value of 6.0, 30-37 deg.C, shading, sealing, standing for anaerobic culture for 48-72 hr;
3. lactobacillus plantarum, a culture medium formula and culture conditions are as follows:
20g of sucrose, 5g of yeast extract, 2g of dipotassium phosphate, 10g of casein peptone, 10g of beef extract, 5g of sodium acetate, 2g of diamine citrate, 801 g of tween, 0.2g of magnesium sulfate, 0.05g of manganese sulfate and the balance of sterile water to 1L; the culture conditions are as follows: initial pH value of 6.0, 30-37 deg.C, shading, sealing, standing for anaerobic culture for 48-72 hr;
4. lactobacillus delbrueckii, culture medium formula and culture conditions:
20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L; the culture conditions are as follows: initial pH value of 6.0, 30-37 deg.C, shading, sealing, and standing for anaerobic culture for 48-72 hr.
The formula and culture conditions of the liquid anaerobic bacteria culture medium for mixed fermentation are as follows:
20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L; the culture conditions are as follows: initial pH value of 6.0, 30-37 deg.C, shading, sealing, and standing for anaerobic culture for 48-72 hr.
The A, B, C1 and C2 are mixed according to the proportion of 1-97 percent respectively to prepare the comprehensive preparation. The proportion of mixing can be adjusted according to the requirements of increasing the yield, preventing and treating plant diseases and insect pests, improving soil and degrading pollution residues.
The adsorption material of the comprehensive preparation consists of the following 4 materials in proportion:
calcined vermiculite powder accounting for 50-97% by mass; vermiculite is a natural mineral substance, is microporous, has high water absorption, is easy for strain preservation, and has medium trace element content as high as 80 percent.
Humic acid accounting for 1-48% by mass; humic acid is a natural high organic matter fertilizer, can fertilize soil, improves fertilizer efficiency, promotes growth and enhances stress resistance.
Turfy soil accounting for 1-48% by mass; the turfy soil is a natural organic fertilizer with high humus content.
1-48% of attapulgite by mass; the silicon content of the attapulgite exceeds 60 percent, and the attapulgite also contains potassium and rare element molybdenum.
The comprehensive preparation can be used for propagation for 2 times. For the first propagation, each kilogram of the comprehensive preparation is added with 10 to 20 liters of 4 to 6 percent brown sugar water, stirred evenly, and ventilated and stirred for 2 to 3 days at 180r/min in a sun-shading way for 120 r/min, thus obtaining the first propagation-expanding microbial inoculum. And (3) performing secondary propagation, namely adding 10-20 liters of 4-6% brown sugar water per liter of the microbial inoculum obtained by the primary propagation, uniformly mixing, shading, and ventilating and stirring for 2-3 days at the speed of 180r/min for 120-. After the secondary propagation of the comprehensive preparation by 100 times and 400 times, the comprehensive preparation can be used for spraying, root irrigation, drip irrigation with water or large-area spraying on crops and soil. The preparation after propagation can also be mixed with organic fertilizer and farmyard manure or applied in a superposition way.
The yield of the applied fertilizer is obtained without using the fertilizer and by applying an organic fertilizer alone, and the dosage of the organic fertilizer reaches or exceeds 8-10 times of that of the fertilizer. And if the organic fertilizer is mixed with the microbial inoculum which is propagated twice and then applied, the yield which is 8-10 times that of the single applied organic fertilizer can be obtained. The amount of the microbial inoculum added in the organic fertilizer for secondary propagation can be the same as or even lower than that of the fertilizer pesticide, and the yield reaches or is higher than that of the fertilizer pesticide for planting under the same condition. The fertilizer and pesticide can be replaced in yield.
The preparation adopts an open fermentation technology, so that the production cost is greatly reduced; a high-performance adsorption material is adopted to adsorb the liquid microbial inoculum, so that the lasting activity of the microbial inoculum is guaranteed; through the secondary propagation of 100 times of the comprehensive preparation, the effect is improved, and the price is reduced. Under the same conditions, the same money can obtain better benefits than the planting of chemical fertilizers and pesticides, and the chemical fertilizers and pesticides can be completely replaced in price.
The safety level of the strains adopted by the comprehensive preparation is determined to be four levels by national authentication departments, and the strains are safe and harmless to human, livestock and ecological environment. These strains are deposited in units recognized in China and internationally as having the qualifications for microbial preservation for patent procedures, specifically in the China center for agricultural microbial culture Collection, the China center for general microbial culture Collection, and the Guangdong center for microbial culture Collection.
Due to the comprehensive effect of the preparation, the diseases of crops are reduced in the whole growth process, the soil is improved, the quality of the crops is improved, the taste returns to natural, no agricultural chemical residue exists, and the preparation can completely replace chemical fertilizers and pesticides in quality.
Example one
Time: 7-10 months in 2019
A place: guangxi Wuxuan county east ditch village
In the ancient and old leather paddies, 20 mu of late rice is planted, 6 mu of demonstration fields are drawn to adopt the comprehensive preparation, and the rest are control fields. The planting variety is Guangxi traditional rice 'Tiandong Xiang'. The secondary propagation expanding preparation is applied to the demonstration field for 4 times, 1, the seedlings are raised, and the seedlings are sprayed for 0.5L/mu; 2. harrowing, namely spreading organic fertilizer 100 k/mu, overlapping and spraying 20L of organic fertilizer, and carrying out rotary tillage harrowing; 3. spraying 2L/mu in the tillering stage; 4. and 2L/mu is sprayed in the booting stage. Fertilizer input: the preparation for secondary propagation is 0.5 yuan/kgx 24.5.5 + 1 yuan/kgx 100 yuan 113 yuan/mu of organic fertilizer. When harvested, the corn is produced by 1100 jin/mu. In a control field, 180 yuan per bag of the emulational brand 3 x 15 compound fertilizer is applied, 5kgx6 yuan per kg of potash fertilizer is applied, and 20 yuan per mu of pesticide is 230 yuan per mu. When harvested, the rice can be produced by 960 jin/mu. No fertilizer or pesticide was applied in the demonstration field, and all the produced rice was purchased by Hongkong Tang organic food company.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (6)
1. An agricultural microbial comprehensive preparation is characterized by being prepared from the following raw materials in parts by weight:
1-97% of A preparation, 1-97% of B preparation, 1-97% of C1 preparation and 1-97% of C2 preparation;
the preparation A comprises rhizobia, azospirillum, bacillus megaterium, bacillus mucilaginosus and an adsorbing material;
the preparation B comprises Bacillus thuringiensis Israeli subspecies, Trichoderma harzianum, Streptomyces microflavus, Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea, Paecilomyces lilacinus, Trichoderma viride and an adsorbing material;
the C1 preparation comprises bacillus subtilis, bacillus licheniformis, bacillus pumilus, trichoderma pseudokoningii and an adsorbing material;
the C2 preparation comprises Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus delbrueckii and adsorbing material.
2. The agricultural microbial complex formulation of claim 1,
the rhizobium culture medium formula comprises the following raw materials in parts by weight: 1.0 percent of molasses, 0.05 percent of dipotassium phosphate, 0.3 percent of calcium carbonate, 0.02 percent of magnesium sulfate, 0.04 percent of yeast extract, 0.0002 percent of sodium molybdate and sterile water for supplementing 100 percent;
the formula of the nitrogen-fixing spirochete culture medium is composed of the following raw materials in parts by weight: 1.0 percent of molasses, 0.01 percent of dipotassium hydrogen phosphate, 0.04 percent of monopotassium phosphate, 0.02 percent of magnesium sulfate, 0.01 percent of sodium chloride, 0.002 percent of calcium chloride, 0.002 percent of ferrous sulfate, 0.0002 percent of sodium molybdate and sterile water which is up to 100 percent;
the bacillus megaterium culture medium formula consists of the following raw materials in percentage by weight: 1.0 percent of corn starch, 0.6 percent of molasses, 1.5 percent of beef extract, 0.1 percent of ammonium sulfate, 0.2 percent of dipotassium hydrogen phosphate, 0.1 percent of calcium carbonate, 0.05 percent of magnesium sulfate, 0.02 percent of zinc sulfate and the balance of sterile water to 100 percent;
the bacillus mucilaginosus culture medium formula consists of the following raw materials in percentage by weight: 2% of molasses, 0.5% of soybean meal powder, 0.1% of magnesium sulfate, 0.05% of dipotassium phosphate, 0.1% of calcium carbonate and the balance of sterile water to 100%.
3. The agricultural microbial complex formulation of claim 1,
the bacillus thuringiensis Israeli subspecies culture medium formula comprises the following raw materials in parts by weight: 2.0 percent of molasses, 0.5 percent of peptone, 0.5 percent of yeast extract, 0.03 percent of dipotassium phosphate, 0.1 percent of disodium phosphate, 0.1 percent of magnesium sulfate, 0.002 percent of ferrous sulfate, 0.002 percent of calcium chloride and the balance of sterile water to 100 percent;
the formula of the trichoderma harzianum culture medium comprises the following raw materials in parts by weight: 20% of potato cooking juice, 0.3% of dipotassium phosphate, 2.0% of sugar, 0.15% of magnesium sulfate, 0.1% of vitamin and sterile water for supplementing 100%;
the culture medium formula of the streptomyces microflavus comprises the following raw materials in parts by weight: 2.0% of soluble starch, 0.1% of potassium nitrate, 0.05% of dipotassium phosphate, 0.1% of magnesium sulfate, 0.001% of ferrous sulfate, 0.05% of sodium chloride and sterile water for supplementing 100%;
the formula of the beauveria bassiana culture medium comprises the following raw materials in parts by weight: 5.0 percent of molasses, 0.1 percent of dipotassium phosphate, 1 percent of corn leaching liquor and sterile water which are complemented to 100 percent;
the formula of the metarhizium anisopliae culture medium comprises the following raw materials in parts by weight: 4.0 percent of molasses, 1.0 percent of peptone, 1.0 percent of yeast extract, 0.15 percent of dipotassium phosphate, 0.05 percent of magnesium sulfate, 0.03 percent of sodium borate, 0.04 percent of manganese sulfate, 0.05 percent of sodium molybdate, 0.08 percent of copper sulfate, 0.4 percent of zinc sulfate, 0.01 percent of ferrous sulfate, trace vitamin B1 and sterile water which is up to 100 percent;
the isaria fumosorosea culture medium formula consists of the following raw materials in percentage by weight: 2.0% of molasses, 0.3% of dipotassium phosphate, 0.15% of magnesium sulfate and 20% of potato extract to make up 100%;
the formula of the lilac paecilomyces culture medium comprises the following raw materials in parts by weight: 1.5 percent of molasses, 0.5-1.0 percent of soluble starch, 0.5 percent of ammonium sulfate, 0.1 percent of dipotassium phosphate, 0.01 percent of zinc sulfate, 0.025 percent of calcium carbonate, 0.001 percent of manganese sulfate and the balance of sterile water to 100 percent;
the formula of the trichoderma viride culture medium comprises the following raw materials in parts by weight: 1.0 percent of molasses, 1.3 percent of corn flour, 0.5 percent of soybean flour, 0.3 percent of dipotassium phosphate, 0.2 percent of ammonium nitrate and sterile water which make up to 100 percent.
4. The agricultural microbial complex formulation of claim 1,
the bacillus subtilis culture medium formula comprises the following raw materials in parts by weight: 1.5 percent of molasses, 0.05 percent of dipotassium phosphate, 0.05 percent of yeast extract, 0.02 percent of beef extract, 0.05 percent of peptone, 0.025 percent of sodium chloride, 0.001 percent of manganese sulfate and the balance of sterile water to 100 percent;
the culture medium formula of the bacillus licheniformis is composed of the following raw materials in parts by weight: 1.5 percent of molasses, 1.0 percent of yeast extract, 0.3 percent of magnesium sulfate, 0.1 percent of beef extract, 0.05 percent of peptone, 0.025 percent of calcium carbonate, 0.02 percent of sodium chloride and the balance of sterile water to 100 percent;
the bacillus pumilus culture medium formula comprises the following raw materials in parts by weight: molasses 0.5%, corn flour 0.5%, peptone 1.5%, bean cake powder 0.5%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.2%, and sterile water to make up 100%;
the trichoderma pseudokoningii culture medium formula consists of the following raw materials in percentage by weight: molasses 0.75%, wheat bran 3.0%, dipotassium hydrogen phosphate 0.1%, corn flour 3.0%, and sterile water to make up 100%.
5. The agricultural microbial complex formulation of claim 1,
the lactobacillus bulgaricus culture medium formula consists of the following raw materials in parts by weight: 20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L;
the lactobacillus acidophilus culture medium formula consists of the following raw materials in parts by weight: 20g of sucrose, 20g of peptone, 1g of sodium chloride, 0.01g of manganese sulfate, 0.02g of magnesium sulfate, 0.5g of sodium acetate and the balance of sterile water to 1L;
the lactobacillus plantarum culture medium formula consists of the following raw materials in parts by weight: 20g of cane sugar, 5g of yeast extract, 2g of dipotassium phosphate, 10g of casein peptone, 10g of beef extract, 5g of sodium acetate, 2g of diamine citrate, 801 g of tween, 0.2g of magnesium sulfate and 0.05g of manganese sulfate,
sterile water to make up 1L;
the lactobacillus delbrueckii culture medium formula consists of the following raw materials in weight gram: 20g of sucrose, 35g of peptone, 1.5g of calcium carbonate, 30g of yeast extract, 2g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate, 1g of calcium carbonate, 0.02g of manganese sulfate, 1.5g of ammonium hydrogen phosphate and the balance of sterile water to 1L.
6. The integrated preparation according to any one of claims 1 to 5, wherein the adsorbent material is prepared from the following raw materials in parts by weight: 50-97% of calcined vermiculite powder, 1-47% of humic acid, 1-47% of turfy soil and 1-47% of attapulgite.
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