CN111937674A - Method for cultivating cordyceps sinensis by taking momordica grosvenori as container - Google Patents
Method for cultivating cordyceps sinensis by taking momordica grosvenori as container Download PDFInfo
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- CN111937674A CN111937674A CN202010925587.2A CN202010925587A CN111937674A CN 111937674 A CN111937674 A CN 111937674A CN 202010925587 A CN202010925587 A CN 202010925587A CN 111937674 A CN111937674 A CN 111937674A
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- Prior art keywords
- cordyceps
- momordica grosvenori
- mycelia
- cordyceps sinensis
- fruits
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- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 120
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A method for culturing Cordyceps in a container of fructus Siraitiae Grosvenorii comprises placing fructus Siraitiae Grosvenorii as a culture container, fructus Siraitiae Grosvenorii pulp as a nutrition source, and various food materials as adjuvants, inoculating Cordyceps strain into fructus Siraitiae Grosvenorii, and culturing until Cordyceps mycelium grows over the whole fructus Siraitiae Grosvenorii and even grows out Cordyceps fruiting body to obtain a fructus Siraitiae Grosvenorii Cordyceps complex. By implementing the method, fresh momordica grosvenori fruits can be placed in the inoculation box, and cordyceps sinensis strains are directly inoculated into the momordica grosvenori fruits after fumigating and sterilizing the fruits, so that the conventional high-temperature high-pressure or normal-pressure sterilization process can be omitted, time and labor are saved, and the production cost is greatly saved. Various food materials are put into the grosvenor momordica body, and cordyceps strains are inoculated into the grosvenor momordica fruit, so that cordyceps mycelia overgrow the whole grosvenor momordica fruit body and even grow cordyceps sporophores, a grosvenor momordica and cordyceps complex is obtained, and the health-care food which has multiple functions and characteristics of delicacy, leisure, food therapy, health preservation and the like is prepared.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a method for cultivating cordyceps sinensis by taking momordica grosvenori as a container.
Background
Momordica grosvenori, a perennial vine of the Cucurbitaceae family, is a famous local specialty of Guilin. The fruits of the traditional Chinese medicine are used as medicines, contain medicinal components such as mogroside, various amino acids, vitamins and the like, and are mainly used for treating cough caused by lung heat and phlegm fire, sphagitis, tonsillitis, acute gastritis, constipation and the like. Lo Han Guo is widely used as sweetener due to its high sweetness and low calorie, and is often used as sugar substitute for obese patients and diabetic patients. The momordica grosvenori fruits contain abundant glucose, fructose, various vitamins and the like, have wide application, are popular in domestic and foreign markets, enjoy high reputation in international markets, and are praised as "immortal fruit" by people.
The functional indications are as follows:
clearing heat and moistening lung, relieving cough, relieving sore throat, and relaxing bowels. Can be used for treating cough due to lung fire dryness, pharyngalgia, aphonia, and constipation due to intestinal dryness.
The pharmacological action is as follows:
(1) cough relieving effect: d-mannitol has antitussive effect. It can also be used for treating cerebral edema, and has effects of increasing blood osmotic pressure, reducing intracranial pressure, and dewatering stronger than urea, and has long lasting time. It can also be used for treating edema caused by large-area burn and scald, preventing and treating acute renal failure, reducing intraocular pressure, treating acute glaucoma, and replacing sugar as sweet food or flavoring agent for diabetic patients.
(2) Has effects on intestinal canal: the grosvenor momordica health tea (containing 77.5 percent of grosvenor momordica, 15 percent of tea and 7.5 percent of grosvenor momordica preparation) has no obvious influence on the spontaneous activity of the isolated small intestine of the white mouse, but can strengthen the spontaneous activity of the isolated small intestine of the rabbit and the dog. Has antagonistic action on intestinal canal tonic contraction caused by acetylcholine or barium chloride to relax intestinal canal and relieve spasm, and also has antagonistic action on intestinal canal relaxation caused by epinephrine to restore spontaneous activity of intestinal canal. The fructus momordicae body building tea is proved to have a bidirectional regulating effect on the intestinal canal motor function.
(3) Other functions are as follows: the grosvener siraitia body-building tea has no influence on the bioelectricity of the stomach of a normal rabbit, namely the normal motor function of the digestive tract. The momordica grosvenori body-building tea has no obvious influence on blood pressure and electrocardiogram when being used for stomach irrigation of anaesthetized dogs.
The efficacy and the effect are as follows:
(1) fructus momordicae is sweet in taste and cool in nature, enters lung and large intestine channels, has the effects of moistening lung to arrest cough, promoting the production of body fluid to quench thirst, is suitable for lung heat or cough with dryness of lung, pertussis, body fluid impairment caused by summer heat, thirst and the like, and also has the effect of relaxing bowel.
(2) Fructus Siraitiae Grosvenorii has effects of clearing heat and moistening lung, and can be used for treating pertussis, cough due to phlegm fire, blood dryness and constipation; the root of the beverage is mashed and then applied to the affected part, so that the beverage can treat stubborn dermatitis, carbuncle swelling, sore and furuncle, the fruit hairs can be used as a medicine for incised wound, and a little momordica grosvenori is soaked in boiled water, so that the beverage is a good cool beverage, can refresh the body and promote the production of body fluid, can prevent respiratory tract infection, and can prolong the life after being taken for a long time. Fructus Siraitiae Grosvenorii juice can also be used for cooking, and has good taste. Has good curative effect on treating acute tracheitis, acute tonsillitis, throat fire and acute gastritis.
(3) The momordica grosvenori is a famous and precious traditional Chinese medicine for both medicine and food, which is published by Ministry of health, contains momordica grosvenori glycoside which is 300 times sweeter than sucrose, does not generate heat, is a famous and precious raw material in beverage and candy industries, and is an optimal substitute for sucrose. The momordica grosvenori tea can prevent various diseases after being drunk frequently, and modern medicine proves that the momordica grosvenori has obvious curative effect on bronchitis, hypertension and other diseases and also has the function of preventing and treating coronary heart disease, angiosclerosis and obesity.
At present, the cold tea brewed by using the fruits of the momordica grosvenori is popular for a long time and can be used as a condiment for stew. The deep processing products mainly comprise momordica grosvenori solid beverage, momordica grosvenori drink, cake prepared from momordica grosvenori, candy, biscuit and the like.
The invention patent of Huangxiying applied in 2016, 6/29: a Cordyceps biological beverage (application number: CN 201610485800.6) is prepared by using fructus Siraitiae Grosvenorii extract as carrier. The technical scheme is that a grosvenor momordica fruit extracting solution is used as a liquid culture medium, cordyceps strains are inoculated into the sterilized liquid culture medium of grosvenor momordica fruit, and then the liquid culture medium of grosvenor momordica fruit is cultivated until cordyceps mycelia or fungus balls grow over the liquid culture medium of grosvenor momordica fruit to obtain the biological drink of grosvenor momordica fruit and cordyceps.
Disclosure of Invention
The invention aims to provide a brand-new method for cultivating cordyceps sinensis by taking momordica grosvenori as a container. The method specifically comprises the following steps: the method comprises the steps of taking fructus momordicae fruits as a cultivation container, taking pulp of fructus momordicae as a nutrition source, taking various food materials as auxiliary materials, placing the food materials in the fructus momordicae, inoculating cordyceps sinensis strains into the fructus momordicae, and cultivating until cordyceps sinensis mycelia grow over the whole fructus momordicae and even grow out cordyceps sinensis sporophores, so as to obtain a fructus momordicae and cordyceps sinensis complex.
The invention is realized by the following technical scheme:
1. a method for culturing cordyceps sinensis by taking momordica grosvenori as a container comprises the following steps: the method comprises the steps of taking fructus momordicae fruits as a cultivation container, taking pulp of fructus momordicae as a nutrition source, taking various food materials as auxiliary materials, placing the food materials in the fructus momordicae, inoculating cordyceps sinensis strains into the fructus momordicae, and cultivating until cordyceps sinensis mycelia grow over the whole fructus momordicae and even grow out cordyceps sinensis sporophores, so as to obtain a fructus momordicae and cordyceps sinensis complex.
2. A method for culturing cordyceps by taking momordica grosvenori as a container, which adopts the technical scheme that momordica grosvenori fruits can be fresh momordica grosvenori or dry momordica grosvenori.
3. A method for culturing cordyceps sinensis by taking momordica grosvenori as a container adopts the technical scheme that the method for collecting and processing the momordica grosvenori comprises the following steps:
(1) collecting and processing fresh momordica grosvenori: collecting fresh momordica grosvenori with the maturity of more than eighty percent as a carrier, then flatly paving momordica grosvenori fruits in a room, ventilating and airing until the surfaces of the fruits are yellow, and evaporating the water content to remove 15-20% of the fruit weight for later use;
(2) collecting and processing dried momordica grosvenori: collecting dried fructus Siraitiae Grosvenorii with undamaged pericarp as carrier, soaking fructus Siraitiae Grosvenorii fruit in starch paste, and naturally drying.
4. A method for culturing Cordyceps in a container with fructus Siraitiae Grosvenorii is provided, and the technical scheme is that the collected food materials comprise one or more of various grains, coarse cereals, fruits and vegetables, animal meat, and Chinese herbal medicines.
5. A method for culturing cordyceps sinensis by taking fructus momordicae as a container adopts the technical scheme that the method for culturing cordyceps sinensis mycelia and cordyceps sinensis sporocarp comprises the following steps:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) direct inoculation: placing undamaged fresh momordica grosvenori fruits in an inoculation box, fumigating and sterilizing the whole inoculation box and momordica grosvenori fruits, then cutting off one end of the momordica grosvenori fruits in an aseptic environment, pricking a plurality of holes in the momordica grosvenori fruits by using a tool, then inoculating a proper amount of cordyceps sinensis strains into the momordica grosvenori fruits, and finally placing the inoculated momordica grosvenori fruits in a culture box for antifouling protection and later-stage culture;
(3) fruit body packaging food materials and sterilization and inoculation: preparing a nutrient solution, placing food materials in the nutrient solution, soaking for 10-12 hours, fishing out, cutting off one end of a fresh fructus momordicae fruit or a dry fructus momordicae fruit, pricking holes in the fructus momordicae fruit by using a tool, then filling the food materials which absorb the nutrient solution into the fructus momordicae fruit body, sterilizing according to a conventional method, then placing the sterilized fructus momordicae in an inoculation chamber, and inoculating a proper amount of cordyceps sinensis strains into the fructus momordicae in an aseptic environment;
(4) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(5) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
The cordyceps, also called cordyceps militaris, is an edible fungus rich in nutrients such as cordycepin, cordycepic acid, cordyceps polysaccharide, high protein, amino acid and the like, and is a high-quality nourishing product. At present, the artificially cultivated cordyceps militaris mainly comprises two types: one is that the cordyceps strains are inoculated into the silkworm pupa which is artificially bred and cultivated to grow a silkworm pupa cordyceps character base, the second is the most common cordyceps flower in the market, and the cordyceps strains are inoculated into cereals, wheat and the like and cultured to obtain cordyceps fruiting bodies. The cordyceps militaris and the cordyceps sinensis belong to the genus cordyceps of the phylum mycomycota, and have similar medicinal and nourishing effects, and a large number of scientific researches prove that the edible and medicinal values of the cordyceps militaris can be comparable with those of the traditional cordyceps sinensis, and the cordycepin content in the artificially cultured cordyceps militaris is dozens of times higher than that of the traditional cordyceps sinensis. Has effects in nourishing lung, invigorating kidney, stopping bleeding, eliminating phlegm, dilating trachea, tranquilizing mind, resisting bacteria, and lowering blood pressure. The research and detection of various countries in the world prove that the cordyceps militaris is the most ideal substitute of the traditional cordyceps militaris.
The invention has the following advantages:
1. the method for cultivating the cordyceps sinensis by taking the momordica grosvenori fruits as the containers has novel conception, simple production process and easy implementation.
2. By implementing the method, fresh momordica grosvenori fruits can be placed in an inoculation box, then the fruits are fumigated and sterilized, microorganisms on the surfaces of fruit peels are killed, one end of the momordica grosvenori fruits are cut off in an aseptic environment, cordyceps sinensis strains are directly inscribed in the momordica grosvenori fruits, the conventional high-temperature high-pressure or normal-pressure sterilization process can be omitted, time and labor are saved, the production cost is greatly saved, and the momordica grosvenori fruits can be eaten through the whole momordica grosvenori fruits quickly due to the fact that the humidity environment in the momordica grosvenori fruits is moderate and the momordica grosvenori fruits are rich in nutrition, hyphae grow rapidly under the rich nutrition of the momordica grosvenori fruits, and when the hyphae are converted into fruit bodies, the.
3. By implementing the method, the dried momordica grosvenori fruits are soaked in the starch paste and then naturally dried, so that the toughness of the momordica grosvenori peels is effectively improved, the fruits are not easy to damage, hypha in the fruits are not easy to pollute during the culture in the middle and later periods, and the value of the commodities is mutually improved by the complete fruits.
4. By implementing the method, various food materials are filled into the grosvenor momordica body, and cordyceps sinensis strains are inoculated into the grosvenor momordica fruit, so that cordyceps sinensis mycelia grow over the whole grosvenor momordica fruit body and even grow cordyceps sinensis sporophores, and a grosvenor momordica and cordyceps sinensis complex is obtained. The prepared fructus momordicae cordyceps militaris complex simultaneously has the original nutrition and functional components of the fructus momordicae fruit body, various food materials and cordyceps militaris, does not lose the original taste of the fructus momordicae, and is a health-care food which simultaneously has multiple functions and characteristics of delicacy, leisure, food therapy, health preservation and the like.
5. By implementing the method, the value of the momordica grosvenori can be increased without limit, the current situations that the momordica grosvenori deep-processed products in the current market have no characteristics and poor value-added effect and the like are changed, the postpartum way of the momordica grosvenori is solved, the development of related industries of the momordica grosvenori is driven, a new economic growth point is formed, and great economic benefits and social benefits are achieved.
6. By implementing the invention, the formed fructus momordicae cordyceps militaris complex is novel and special, has high nutritional value and obvious health care effect, and can be used for making tea, making wine and cooking soup or processing the fructus momordicae cordyceps militaris complex into other special foods. People can achieve the effects of food therapy, health care and health preservation when people take leisure food regularly. Therefore, the invention not only has good market prospect, but also has significant economic benefit.
Detailed Description
The process of the present invention is further illustrated below with reference to examples.
A method for culturing cordyceps sinensis by taking momordica grosvenori as a container comprises the following steps: the method comprises the steps of taking fructus momordicae fruits as a cultivation container, taking pulp of fructus momordicae as a nutrition source, taking various food materials as auxiliary materials, placing the food materials in the fructus momordicae, inoculating cordyceps sinensis strains into the fructus momordicae, and cultivating until cordyceps sinensis mycelia grow over the whole fructus momordicae and even grow out cordyceps sinensis sporophores, so as to obtain a fructus momordicae and cordyceps sinensis complex.
The first embodiment is as follows:
1. collecting momordica grosvenori: collecting fresh momordica grosvenori with the maturity of more than eighty percent as a carrier, then flatly paving momordica grosvenori fruits in a room, ventilating and airing until the surfaces of the fruits are yellow, and evaporating the water content to remove 15-20% of the fruit weight for later use;
2. the method for cultivating the cordyceps mycelia and the cordyceps fruiting bodies comprises the following steps:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) direct inoculation: placing undamaged fresh momordica grosvenori fruits and related tools in an inoculation box, fumigating and disinfecting the whole inoculation box, momordica grosvenori fruit bodies and the related tools for 30-50 minutes, then cutting off one end of the momordica grosvenori fruits in an aseptic environment, pricking the momordica grosvenori fruits through holes by using the tools, then inoculating a proper amount of cordyceps liquid strains into the momordica grosvenori fruits, and finally placing the inoculated momordica grosvenori fruits in a culture box for antifouling protection and later-stage culture;
(3) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(4) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
Example two:
1. collecting momordica grosvenori: collecting fresh momordica grosvenori with the maturity of more than eighty percent as a carrier, then flatly paving momordica grosvenori fruits in a room, ventilating and airing until the surfaces of the fruits are yellow, and evaporating the water content to remove 15-20% of the fruit weight for later use;
2. rice is selected as an auxiliary material.
3. The method for cultivating the cordyceps mycelia and the cordyceps fruiting bodies comprises the following steps:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) fruit body packaging food materials and sterilization and inoculation: preparing a nutrient solution, placing rice in the nutrient solution, soaking for 10-12 hours, fishing out, removing one end of a momordica grosvenori fruit, pricking a plurality of holes in the momordica grosvenori fruit by using a tool, filling the rice which absorbs the nutrient solution into the momordica grosvenori fruit body, sterilizing according to a conventional method, placing the sterilized momordica grosvenori into an inoculation chamber, and inoculating a proper amount of cordyceps sinensis strains into the momordica grosvenori in a sterile environment;
(3) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(4) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
Example three:
1. collecting and processing momordica grosvenori: collecting the dried fructus momordicae with undamaged pericarp as a carrier, then placing glutinous rice into water according to the proportion of putting 5-10 jin of glutinous rice powder into 100 jin of water, heating and decocting the glutinous rice into rice paste, then soaking the fructus momordicae fruit into the rice paste for 1-2 minutes, and naturally airing the fructus momordicae for later use, thereby effectively improving the toughness of the fructus momordicae pericarp and ensuring that the pericarp is not easy to damage.
2. Rice is selected as an auxiliary material.
3. The method for cultivating the cordyceps mycelia and the cordyceps fruiting bodies comprises the following steps:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) fruit body packaging food materials and sterilization and inoculation: preparing a nutrient solution, placing rice in the nutrient solution, soaking for 10-12 hours, fishing out, removing one end of a momordica grosvenori fruit, pricking a plurality of holes in the momordica grosvenori fruit by using a tool, filling the rice which absorbs the nutrient solution into the momordica grosvenori fruit body, sterilizing according to a conventional method, placing the sterilized momordica grosvenori into an inoculation chamber, and inoculating a proper amount of cordyceps sinensis strains into the momordica grosvenori in a sterile environment;
(3) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(4) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
Example four:
1. collecting and processing momordica grosvenori: collecting the dried fructus momordicae with undamaged pericarp as a carrier, then placing glutinous rice into water according to the proportion of putting 5-10 jin of glutinous rice powder into 100 jin of water, heating and decocting the glutinous rice into rice paste, then soaking the fructus momordicae fruit into the rice paste for 1-2 minutes, and naturally airing the fructus momordicae for later use, thereby effectively improving the toughness of the fructus momordicae pericarp and ensuring that the pericarp is not easy to damage.
2. Wheat is selected as an auxiliary material.
3. The method for cultivating the cordyceps mycelia and the cordyceps fruiting bodies comprises the following steps:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) fruit body packaging food materials and sterilization and inoculation: preparing a nutrient solution, placing wheat in the nutrient solution, soaking for 10-12 hours, fishing out, removing one end of a momordica grosvenori fruit, pricking a plurality of holes in the momordica grosvenori fruit by using a tool, filling the wheat which is full of the nutrient solution into the momordica grosvenori fruit body, sterilizing according to a conventional method, placing the sterilized momordica grosvenori into an inoculation chamber, and inoculating a proper amount of cordyceps sinensis strains into the momordica grosvenori under a sterile environment;
(3) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(4) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
Example five:
1. collecting and processing momordica grosvenori: collecting the dried fructus momordicae with undamaged pericarp as a carrier, then placing glutinous rice into water according to the proportion of putting 5-10 jin of glutinous rice powder into 100 jin of water, heating and decocting the glutinous rice into rice paste, then soaking the fructus momordicae fruit into the rice paste for 1-2 minutes, and naturally airing the fructus momordicae for later use, thereby effectively improving the toughness of the fructus momordicae pericarp and ensuring that the pericarp is not easy to damage.
2. The comprehensive auxiliary materials are selected from 10-20 parts of dehydrated coix seeds, 5-10 parts of red dates, 6-8 parts of medlar, 3-5 parts of oyster meat, 3-5 parts of sea cucumber meat, 2-3 parts of ginseng and 2-3 parts of cistanche deserticola, and are mixed.
3. The method for cultivating the cordyceps mycelia and the cordyceps fruiting bodies comprises the following steps:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) fruit body packaging food materials and sterilization and inoculation: preparing a nutrient solution, placing the comprehensive auxiliary materials into the nutrient solution, soaking for 10-12 hours, fishing out, cutting off one end of the fructus momordicae fruit, pricking holes in the fructus momordicae fruit by using a tool, filling the comprehensive auxiliary materials which absorb the nutrient solution into the fructus momordicae fruit body, sterilizing according to a conventional method, placing the sterilized fructus momordicae into an inoculation chamber, and inoculating a proper amount of cordyceps sinensis strains into the fructus momordicae in a sterile environment;
(3) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(4) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
As described above, it will be apparent to those skilled in the art that other various modifications can be made based on the technical solution and the technical idea of the present invention, and all such modifications shall fall within the scope of the appended claims.
Claims (5)
1. A method for culturing cordyceps sinensis by taking momordica grosvenori as a container is characterized by comprising the following steps: the method comprises the steps of taking fructus momordicae fruits as a cultivation container, taking pulp of fructus momordicae as a nutrition source, taking various food materials as auxiliary materials, placing the food materials in the fructus momordicae, inoculating cordyceps sinensis strains into the fructus momordicae, and cultivating until cordyceps sinensis mycelia grow over the whole fructus momordicae and even grow out cordyceps sinensis sporophores, so as to obtain a fructus momordicae and cordyceps sinensis complex.
2. The method for cultivating cordyceps sinensis in a container according to claim 1, wherein the momordica grosvenori fruits can be either fresh momordica grosvenori or dry momordica grosvenori.
3. The method for cultivating cordyceps sinensis by taking momordica grosvenori as a container according to claim 1, wherein the collection and processing method of momordica grosvenori is as follows:
(1) collecting and processing fresh momordica grosvenori: collecting fresh momordica grosvenori with the maturity of more than eighty percent as a carrier, then flatly paving momordica grosvenori fruits in a room, ventilating and airing until the surfaces of the fruits are yellow, and evaporating the water content to remove 15-20% of the fruit weight for later use;
(2) collecting and processing dried momordica grosvenori: collecting dried fructus Siraitiae Grosvenorii with undamaged pericarp as carrier, soaking fructus Siraitiae Grosvenorii fruit in starch paste, and naturally drying.
4. The method for cultivating cordyceps sinensis by taking momordica grosvenori as a container according to claim 1, wherein the collected food materials comprise one or more of various grains, coarse cereals, fruits and vegetables, animal meat and Chinese herbal medicines.
5. The method for cultivating cordyceps sinensis by taking momordica grosvenori as a container according to claim 1, wherein the cultivation method of cordyceps sinensis mycelia and cordyceps sinensis fruiting bodies is as follows:
(1) collecting cordyceps sinensis strains: collecting cordyceps sinensis strains with white hyphae, strong wall climbing force, vigorous hyphae and strong as inoculation strains;
(2) direct inoculation: placing undamaged fresh momordica grosvenori fruits in an inoculation box, fumigating and sterilizing the whole inoculation box and momordica grosvenori fruits, then cutting off one end of the momordica grosvenori fruits in an aseptic environment, pricking a plurality of holes in the momordica grosvenori fruits by using a tool, then inoculating a proper amount of cordyceps sinensis strains into the momordica grosvenori fruits, and finally placing the inoculated momordica grosvenori fruits in a culture box for antifouling protection and later-stage culture;
(3) fruit body packaging food materials and sterilization and inoculation: preparing a nutrient solution, placing food materials in the nutrient solution, soaking for 10-12 hours, fishing out, cutting off one end of a fresh fructus momordicae fruit or a dry fructus momordicae fruit, pricking holes in the fructus momordicae fruit by using a tool, then filling the food materials which absorb the nutrient solution into the fructus momordicae fruit body, sterilizing according to a conventional method, then placing the sterilized fructus momordicae in an inoculation chamber, and inoculating a proper amount of cordyceps sinensis strains into the fructus momordicae in an aseptic environment;
(4) the method for cultivating the cordyceps sinensis mycelia comprises the following steps: placing the fructus Siraitiae Grosvenorii inoculated with Cordyceps strain in an environment with room temperature of 16-20 deg.C, humidity of 70-75 and dark light, culturing for 3-5 days, adjusting room temperature environment of the culture chamber to 20-25 deg.C and humidity of 80-90% when mycelia overgrow the material surface, allowing mycelia to be exposed to light for 3-5 days, allowing Cordyceps mycelia to overgrow the whole fructus Siraitiae Grosvenorii, and turning color on the mycelia surface to complete the whole Cordyceps mycelia culture process, and further obtain a fructus Siraitiae Grosvenorii and Cordyceps complex;
(5) the cultivation method of the grass carpophore comprises the following steps: when the cordyceps mycelia overgrow the whole momordica grosvenori and the surface of the mycelia begins to turn color, scratching the surface of the mycelia and culturing continuously at the room temperature of 20-25 ℃ and the humidity of 80-90% in an illumination environment until cordyceps mycelia overgrow in the momordica grosvenori, thereby obtaining another momordica grosvenori and cordyceps complex.
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