CN111924982A - Biological permeable reactive barrier for composite contaminated site and preparation method thereof - Google Patents
Biological permeable reactive barrier for composite contaminated site and preparation method thereof Download PDFInfo
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- CN111924982A CN111924982A CN202010807812.2A CN202010807812A CN111924982A CN 111924982 A CN111924982 A CN 111924982A CN 202010807812 A CN202010807812 A CN 202010807812A CN 111924982 A CN111924982 A CN 111924982A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/002—Reclamation of contaminated soil involving in-situ ground water treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
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Abstract
The invention discloses a biological permeable reactive barrier for a composite pollution site, which comprises a concrete base, a concrete top cover, a reactive barrier cavity and a reactive zone filler, wherein the reactive zone filler comprises a phosphorus-dissolving bacillus mineralization layer, an anaerobic bacillus degradation layer and an aerobic bacillus degradation oxygen generation layer which are sequentially arranged along the seepage direction of underground water; the mineralized layer of the bacillus phosphate solubilizing comprises bacillus phosphate solubilizing, first quartz sand and phosphate rock powder; the anaerobic bacillus degradation layer comprises anaerobic bacillus, second quartz sand and first activated carbon; the aerobic bacillus degradation oxygen release layer comprises aerobic bacillus, peroxide, third quartz sand and second activated carbon; the heavy metal-organic compound pollution remediation can be realized, and the purification capacity is strong; the service strength can be adjusted elastically according to the pollution degree, and the service life of the reaction wall is prolonged; can be used for preventing and treating high-risk sudden pollution sites.
Description
Technical Field
The invention relates to the technical field of groundwater pollution remediation, in particular to a biological permeable reactive barrier for a composite pollution site and a preparation method thereof.
Background
In developing countries driven by industry, complex conditions such as various heavy metals, organic and composite pollution, water and soil composite pollution and the like generally exist. The existing methods for treating polluted underground water comprise in-situ remediation and ex-situ remediation, and the permeable reactive barrier technology is a common in-situ treatment technology in underground water remediation and is constructed perpendicular to the flowing direction of the polluted underground water, and pollutants flowing through the underground water are removed by reaction materials in the permeable reactive barrier. The permeable reactive barrier has the advantages of continuous in-situ treatment of pollutants, treatment of various pollutants, small disturbance, good treatment effect, convenience in installation and construction, relatively high cost performance and the like, so that a plurality of successful practical engineering examples exist in Europe and America at present, wherein the permeable reactive barrier has an organic pollution site and a heavy metal pollution site, but the permeable reactive barrier is less and is efficiently suitable for the organic-heavy metal composite pollution site. In addition, the polluted sites of Yangtze river and yellow river basin in China have the characteristics of complex geological and hydrological conditions, variable pollution degree and sudden polluted sites. Therefore, the permeable reactive barrier not only needs to be repaired in the existing polluted site, but also needs to meet the prevention and treatment of the high-risk sudden polluted site.
The reaction medium permeable in the reaction wall or apatite, zeolite, slag, organophilic clay, biochar and the like mainly used for adsorption; or zero-valent iron with redox properties; or microorganisms that metabolize contaminants in the water. The microorganism permeable reactive barrier has no secondary pollution in the repairing process, is low-carbon and energy-saving, can thoroughly degrade organic pollutants, and has good application prospect.
However, the existing microorganism permeable reactive barrier has the following defects: the remediation effect on heavy metal and heavy metal-organic compound pollution is poor, and the purification capacity is weak; microbial nutrient substances in the permeable reactive wall are continuously consumed, so that the service life of the reactive wall is short; the microbial activity is determined by the content of the existing nutrient substances in the reaction wall, and the adaptive metabolic activity cannot be adopted according to the pollution degree, so that the restoration strength cannot be flexibly adjusted according to the pollution degree for polluted sites with variable pollution degrees; once the microbial reaction wall is put in, the living metabolism of the microbes is started, and the requirement of prevention and control of a high-risk sudden pollution site on standby service of the permeable reaction wall cannot be met.
Therefore, how to prepare a permeable reactive barrier which has strong capacity of purifying heavy metal pollution, can elastically adjust the service strength according to the pollution degree and can meet the prevention and treatment of high-risk sudden pollution sites becomes a technical problem to be solved urgently.
Disclosure of Invention
The invention aims to provide a biological permeable reactive barrier for a composite pollution site and a preparation method thereof, which can realize the restoration of heavy metal-organic matter composite pollution, have strong purification capacity, and can meet the requirement that the service strength of a high-risk sudden pollution site can be elastically adjusted according to the pollution degree.
In order to achieve the purpose, the invention provides a biological permeable reactive barrier for a composite pollution site, which comprises a concrete base, a concrete top cover, a reactive barrier cavity and reactive zone fillers, wherein the reactive barrier cavity is arranged between the concrete base and the concrete top cover, and the reactive zone fillers are arranged in the reactive barrier cavity;
the reaction area filler comprises a phosphorus-dissolving bacillus mineralization layer, an anaerobic bacillus degradation layer and an aerobic bacillus degradation oxygen layer which are sequentially arranged along the groundwater seepage direction;
the raw materials of the mineralized layer of the bacillus phosphate solubilizing comprise bacillus phosphate solubilizing, first quartz sand and phosphate rock powder;
the raw materials of the anaerobic bacillus degradation layer comprise anaerobic bacillus, second quartz sand and first activated carbon;
the raw materials of the aerobic bacillus degradation oxygen layer comprise aerobic bacillus, peroxide, third quartz sand and second activated carbon.
Further, the bacillus phosphate solubilizing bacteria, the anaerobic bacillus and the aerobic bacillus are domesticated to take organic pollutants as required carbon sources and nitrogen sources; the phosphate solubilizing bacillus, the anaerobic bacillus and the aerobic bacillus are purchased bacteria or screened from a polluted site to be repaired.
Further, the particle sizes of the first quartz sand, the second quartz sand and the third quartz sand are all 0.1-3 mm; the particle sizes of the first active carbon and the second active carbon are both 0.05 mm-1 mm.
Further, the thickness of the phosphorizing bacillus mineralization layer, the thickness of the anaerobic bacillus degradation layer and the thickness of the aerobic bacillus degradation oxygen layer are all 30-40 cm.
Further, the preparation method of the mineralized layer of the bacillus phosphate solubilizing bacterium comprises the following steps:
obtaining the bacillus phosphate solubilizing bacteria, the phosphate rock powder and the first quartz sand, wherein the bacillus phosphate solubilizing bacteria is OD6001.5-3 of a bacillus phosphate solubilizing suspension;
and (3) mixing the bacillus phosphate solubilizing bacteria and the phosphate rock powder according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria;
uniformly mixing the phosphate rock powder loaded with the phosphate solubilizing bacillus and the first quartz sand to obtain a phosphate solubilizing bacillus mineralization layer; wherein the volume ratio of the phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria to the first quartz sand is 1: (1-1.5).
Further, the preparation method of the mineralized layer of the anaerobic bacillus comprises the following steps:
obtaining the anaerobic bacillus, the first active carbon and the second quartz sand, wherein the anaerobic bacillus is OD6001.5-3 of an anaerobic bacillus suspension;
mixing the anaerobic bacillus and the first activated carbon according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain first activated carbon loaded with anaerobic bacillus;
uniformly mixing the second quartz sand and the first activated carbon loaded with the anaerobic bacillus to obtain an anaerobic bacillus mineralization layer; wherein the volume ratio of the first activated carbon loaded with anaerobic bacillus to the second quartz sand is 1: (1-1.5).
Further, the preparation method of the aerobic bacillus mineralization layer comprises the following steps:
obtaining the aerobic bacillus, the peroxide, the second active carbon and the third quartz sand, wherein the aerobic bacillus is OD6001.5-3 of aerobic bacillus suspension;
mixing the aerobic bacillus and the second activated carbon according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain second activated carbon loaded with aerobic bacillus;
uniformly mixing the peroxide, the third quartz sand and the second activated carbon loaded with aerobic bacillus to obtain an aerobic bacillus mineralization layer; wherein the volume ratio of the third quartz sand to the peroxide to the aerobic bacillus-loaded second activated carbon is (1-1.5): (0.4-0.7): 1.
further, the reaction wall cavity comprises a first pervious concrete cavity, a second pervious concrete cavity and a third pervious concrete cavity; the phosphate solubilizing bacillus mineralization layer, the anaerobic bacillus degradation layer and the aerobic bacillus degradation oxygen layer are sequentially arranged in the first permeable concrete cavity, the second permeable concrete cavity and the third permeable concrete cavity.
Further, the wall thicknesses of the first pervious concrete cavity, the second pervious concrete cavity and the third pervious concrete cavity are all 15 cm-25 cm.
Furthermore, be equipped with the draw-in groove on the concrete foundation, the draw-in groove includes first draw-in groove, second draw-in groove and third draw-in groove, just first concrete chamber that permeates water, second concrete chamber that permeates water and third concrete chamber that permeates water one-to-one set up on first draw-in groove, second draw-in groove and the third draw-in groove.
The invention also provides a preparation method of the bacillus microorganism permeable reactive barrier prepared by the method, which comprises the following steps:
obtaining a concrete base, and horizontally placing the concrete base;
obtaining a reaction wall cavity, arranging a reaction area filler in the reaction wall cavity, and arranging the reaction wall cavity on the concrete base, wherein the reaction area filler comprises a phosphorus-dissolving bacillus mineralization layer, an anaerobic bacillus degradation layer and an aerobic bacillus degradation oxygen layer which are sequentially arranged along the groundwater seepage direction;
and arranging a concrete top cover at the top of the reaction wall cavity.
One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:
(1) the invention provides a biological permeable reactive barrier for a composite pollution site and a preparation method thereof, the biological permeable reactive barrier consists of a phosphorite dissolving layer for repairing heavy metals, an anaerobic layer for degrading organic pollutants and an aerobic layer of three layers of reaction media, the three layers are paved layer by layer and are advanced, and the heavy metal-organic composite pollutants in polluted underground water can be efficiently removed through the actions of mineralization, adsorption, biodegradation, redox and the like, specifically: the method utilizes the efficient phosphorization of the phosphorus-dissolving bacillus to stably pollute the free heavy metal in the underground water, and utilizes the anaerobic bacillus and the aerobic bacillus to degrade the organic pollutant so as to realize the removal of the heavy metal-organic composite pollution.
(2) Utilize the characteristic that the bacillus produces the dormancy of spore, can satisfy the requirement of high risk sudden pollution place prevention and cure to permeable reactive barrier ready service to pollution place that the pollution degree is changeable, can be according to the flexible regulation service intensity of pollution degree, specifically: the permeable reactive wall sieve is a bacillus that feeds on organic pollutants and can produce spore dormancy in the absence of food. Therefore, when the permeable reactive barrier is positioned in an uncontaminated high-risk sudden compound contaminated site or a compound contaminated site with a reduced pollution degree of underground water, the bacillus lacks a food source and gradually generates spore dormancy; when the site is suddenly polluted compositely or the concentration of organic pollutants in underground water is increased, the bacillus is reactivated and effectively serves. Therefore, the permeable reactive wall surface of the bacillus can be flexibly used for polluted sites with variable pollution degrees, and can be used for standby in the face of uncontaminated high-risk sudden polluted sites, so that the prevention and treatment effect is achieved. The microbial flora has strong adaptability to polluted environment and high reaction activity, and no additional nutrient substances are needed to be added into the reaction wall, so that the rapid loss of a reaction medium is avoided, and the service life of the reaction wall is long.
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In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a biological permeable reactive barrier of a composite pollution site provided by the invention;
FIG. 2 is a flow chart of a method for preparing a biological permeable reactive barrier of a composite pollution site provided by the invention;
1. a concrete base; 11. a first card slot; 12. a second card slot; 13. a third card slot; 2. a concrete top cover; 3. a reaction wall cavity; 31. a first pervious concrete cavity; 32. a second pervious concrete cavity; 33. a third pervious concrete cavity; 4. filling materials in the reaction zone; 41. b, mineralizing the phosphate solubilizing bacillus layer; 42. an anaerobic bacillus degradation layer; 43. the aerobic bacillus is used for reducing oxygen in the oxygen layer;
Detailed Description
The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is a conflict, the present specification will control.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be obtained by an existing method. For the sake of distinction, the terms "first", "second", etc. of the present invention do not denote an order, and may be understood as terms.
The embodiment of the invention provides a biological permeable reactive barrier for a composite pollution site, which has the following general idea:
according to a typical embodiment of the invention, a biological permeable reactive barrier for a composite contaminated site is provided, as shown in fig. 1, the permeable reactive barrier comprises a concrete base 1, a concrete top cover 2 and a reactive zone filler 4, a reactive barrier cavity 3 is arranged between the concrete base 1 and the concrete top cover 2, and the reactive zone filler is arranged in the reactive barrier cavity 3;
the reaction zone filler 4 comprises a phosphorus-dissolving bacillus mineralization layer 41, an anaerobic bacillus degradation layer 42 and an aerobic bacillus degradation oxygen-explaining layer 43 which are sequentially arranged along the groundwater seepage direction;
the raw materials of the Bacillus phosphate solubilizing mineralized layer 41 comprise Bacillus phosphate solubilizing, first quartz sand and phosphate rock powder;
the raw materials of the anaerobic bacillus degradation layer 42 comprise anaerobic bacillus, second quartz sand and first activated carbon;
the raw materials of the aerobic bacillus degradation oxygen layer 43 comprise aerobic bacillus, peroxide, third quartz sand and second activated carbon.
The invention provides a biological permeable reaction wall of a composite pollution site and a preparation method thereof, which consists of three layers of reaction media, namely a phosphorite dissolving layer for repairing heavy metal, an anaerobic layer for degrading organic pollutants and an aerobic layer, wherein the three layers are paved layer by layer and are advanced, and the heavy metal-organic composite pollutants in polluted underground water can be efficiently removed through the actions of mineralization, adsorption, biodegradation, redox and the like; specifically, the method comprises the following steps: the method has the advantages that the efficient phosphorization of the phosphorus-dissolving bacillus is utilized to stably pollute the free heavy metal in the underground water, and the anaerobic bacillus and the aerobic bacillus are utilized to degrade the organic pollutant, so that the heavy metal-organic composite pollution is removed; utilize the characteristic that the bacillus produces the dormancy of spore, can satisfy the requirement of high risk sudden pollution place prevention and cure to permeable reactive barrier ready service to pollution place that the pollution degree is changeable, can be according to the flexible regulation service intensity of pollution degree, specifically: the permeable reactive wall sieve is a bacillus that feeds on organic pollutants and can produce spore dormancy in the absence of food. Therefore, when the permeable reactive barrier is positioned in an uncontaminated high-risk sudden compound contaminated site or a compound contaminated site with a reduced pollution degree of underground water, the bacillus lacks a food source and gradually generates spore dormancy; when the site is suddenly polluted compositely or the concentration of organic pollutants in underground water is increased, the bacillus is reactivated and effectively serves. Therefore, the permeable reactive wall surface of the bacillus can be flexibly used for polluted sites with variable pollution degrees, and can be used for standby in the face of uncontaminated high-risk sudden polluted sites, so that the prevention and treatment effect is achieved. The microbial flora has strong adaptability to polluted environment and high reaction activity, and no additional nutrient substances are needed to be added into the reaction wall, so that the rapid loss of a reaction medium is avoided, and the service life of the reaction wall is long.
The first scheme is as follows: the phosphate solubilizing bacillus, the anaerobic bacillus and the aerobic bacillus are all commercially available microbial agents. Wherein (1) the bacillus phosphate solubilizing bacteria: three or more bacterial species in bacillus with phosphorus dissolving effect, such as bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, bacillus polymyxa and the like. (2) Anaerobic bacillus: comprises three or more than three types of bacteria in anaerobic bacilli such as clostridium butyricum, clostridium perfringens, clostridium difficile, clostridium sporogenes, clostridium botulinum and clostridium pasteurianum. (3) Aerobic bacillus: bacillus anthracis, Bacillus cereus, Bacillus subtilis, Bacillus mycoides, Bacillus polymyxa, Bacillus coagulans, Bacillus laterosporus, Bacillus thuringiensis, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus megaterium, Bacillus brevis, Bacillus lentus, Bacillus pumilus, Bacillus circulans, Bacillus firmus, Bacillus eastern, Bacillus natto, Lactobacillus and other aerobic Bacillus species.
Scheme II: domesticating the purchased phosphorus dissolving bacillus, anaerobic bacillus and aerobic bacillus and taking organic pollutants as required carbon sources and nitrogen sources, specifically: culturing the purchased phosphorus-dissolving bacillus, anaerobic bacillus and aerobic bacillus respectively by using a culture medium and a culture mode suggested in a purchase instruction of a preservation center, gradually replacing nutrient component increment with polluted site underground water, and continuously increasing the concentration of the polluted site underground water; the surviving bacilli in the three culture mediums are separated, preserved or expanded for culture. According to the scheme, the finally-surviving purchased phosphorus-dissolving bacillus, anaerobic bacillus and aerobic bacillus can be more quickly adapted to the severe environment of the polluted site during application, and the gene expression of the bacillus is stronger when organic pollutants in the polluted site are taken as food.
The third scheme is as follows: and screening the phosphate solubilizing bacillus, the anaerobic bacillus and the aerobic bacillus in a to-be-repaired polluted site, and acclimating the phosphate solubilizing bacillus, the anaerobic bacillus and the aerobic bacillus to take organic pollutants as required carbon sources and nitrogen sources. The preparation method comprises the following steps:
(1) enrichment culture of dissolved phosphorus
Preparing an inorganic phosphorus bacteria culture medium: glucose 10.0g, (NH)4)2SO4 0.5g、NaCl 0.3g、KCl 0.3g、MgSO4·7H2O 0.3g、FeSO4·7H2O 0.03g、MnSO4 1.0g、Ca3[PO4]2 5.0g、H2O1000mL, agar 15g
Preparing an organophosphorus bacteria culture medium: glucose 10.0g, (NH)4)2SO4 0.5g、NaCl 0.3g、KCl 0.3g、MgSO4·7H2O 0.3g、FeSO4·7H2O 0.03g、MnSO41.0g, lecithin 5.0g, H21000mL of O, 20g of CaO and 15g of agar;
screening of phosphate solubilizing bacteria: respectively coating the collected soil sample on the two culture media by using a dilution plate method, culturing for 3d at 30 ℃, selecting a single colony which generates a transparent ring around the culture medium, streaking and purifying, and storing on an LB solid slant culture medium.
And fourthly, separation and identification of the bacillus phosphate solubilizing bacteria: and (3) separating and identifying the screened bacillus by using a 16S rRNA/rDNA sequence analysis method, thereby obtaining the phosphorus-solubilizing bacillus taking organic pollutants as carbon sources and nitrogen sources required by the life of bacteria.
(2) Anaerobic enrichment culture
Preparing a common solid culture medium plate (such as LB/NA/NB and the like);
secondly, coating the collected soil samples on culture medium plates respectively by using a dilution plate method, putting the plates into an anaerobic bag, filling an oxygen absorbent, sealing the opening, and culturing in a common incubator for 3 d;
thirdly, the single colony grown is separated and identified by 16S rRNA/rDNA sequence analysis method.
(3) Aerobic enrichment culture
Preparing a common solid culture medium plate (such as LB/NA/NB and the like);
secondly, coating the collected soil samples on culture medium plates respectively by using a dilution plate method, and putting the plates into a common incubator for culture for 3 d;
thirdly, the single colony grown is separated and identified by 16S rRNA/rDNA sequence analysis method.
Since the genus Bacillus is common in nature, the above-mentioned screening and acclimatization method is not necessarily applicable to the microorganism, and is not incidental.
and 4, separating, storing or carrying out amplification culture on the viable bacilli in the three culture media.
The phosphorus-dissolving bacillus, the anaerobic bacillus and the aerobic bacillus are all screened from a polluted site to be repaired and are domesticated for the following reasons: (1) the local bacteria in the field have stronger adaptability to the polluted environment; (2) the indigenous bacillus in the field has the capability of feeding on organic matters in the polluted field; (3) further domestication makes the gene expression of the local bacillus in the field with organic pollutants in the polluted field as food stronger.
As a preferred embodiment, the reaction wall cavity 3 includes a first pervious concrete cavity 31, a second pervious concrete cavity 32 and a third pervious concrete cavity 33; the phosphate solubilizing bacillus mineralization layer 41, the anaerobic bacillus degradation layer 42 and the aerobic bacillus degradation oxygen layer 43 are sequentially arranged in the first permeable concrete cavity 31, the second permeable concrete cavity 32 and the third permeable concrete cavity 33.
In a preferred embodiment, the wall thickness of each of the first, second and third pervious concrete cavities is 15cm to 25 cm. The thickness needs to ensure that the pervious concrete cavity with a certain height has enough strength and is not easy to bend, deform or break, and the larger the pervious concrete cavity is, the larger the required pervious concrete cavity wall thickness is. The height of the pervious concrete cavity is determined according to the specific geological and hydrological conditions, the bottom end of the pervious concrete cavity is embedded into a impervious bed by at least 160cm so as to prevent the pollution plume from flowing to the downstream area under the action of bottom seepage, and the top end of the pervious concrete cavity is higher than the highest water level of underground water.
The water permeating speeds of the first water permeating concrete cavity, the second water permeating concrete cavity and the third water permeating concrete cavity reach 30-50L/m/h, the water permeating speed emphasizes the water permeating performance of the water permeating concrete, and the water permeating concrete with the water permeating performance within the parameter range is required to serve as the cavity. And the concrete determination of the parameters depends on the nature of the soil layer of the polluted site, and the higher the permeability of the soil layer of the site is (sandy soil is more than silty sand is more than sandy silt soil is more than silty clay is more than clay), the higher the permeability of the selected pervious concrete is within the range.
In a preferred embodiment, the thickness of the phosphate solubilizing bacillus mineralization layer, the thickness of the anaerobic bacillus degradation layer and the thickness of the aerobic bacillus degradation oxygen layer are all 30 cm-40 cm. The thickness range is designed according to the speed of the common groundwater flow of the polluted site and the hydraulic retention time of the pollutants with common concentration in the permeable reactive barrier, namely the reaction time required for repairing the pollutants, namely the retention time of the polluted plume in the reactive barrier. The rule that the thickness is thicker with higher contaminant concentration is followed within this thickness range. The pollutant objects corresponding to and repaired by each layer of medium are different, and the thickness of each layer is determined according to the concentration of the pollutant corresponding to each layer. For a certain pollutant concentration, the reaction medium layer is too thin to reach the target reaction effect, and the reaction effect is not improved by too thick, so that the cost is increased, and the cost performance is low.
The degree of compaction, also known as degree of compaction, is the ratio of the dry density of the compacted soil or other road-making material to the standard maximum dry density, expressed as a percentage, in the filling of the individual layers of the reaction medium. After the reaction materials of each layer are mixed in proportion, the reaction materials are filled into the cavity according to the designed compactness. When the filling compactness is low, the reaction medium is loose and has strong penetration; when the packing compactness is high, the reaction medium is dense, and the permeability is reduced when the permeability is looser. During actual operation, the compaction degree is determined according to the permeability of the site soil, and the permeability of each layer of reaction medium is ensured to be slightly larger than the permeability of the site polluted soil.
As a preferred embodiment, the concrete foundation 1 is provided with a clamping groove, the clamping groove includes a first clamping groove 11, a second clamping groove 12 and a third clamping groove 13, and the first permeable concrete cavity 31, the second permeable concrete cavity 32 and the third permeable concrete cavity 33 are correspondingly arranged on the first clamping groove 11, the second clamping groove 12 and the third clamping groove 13.
The concrete base is C30 common concrete, and the height is preferably 30-50 cm; the shapes of the first pervious concrete cavity 31, the second pervious concrete cavity 32 and the third pervious concrete cavity 33 are preferably U-shaped; the reactant media of each layer of the permeable reactive barrier are all borne by the independent U-shaped permeable concrete, so that the permeable reactive barrier has the advantages of draining underground water, preventing the deformation of a reactive barrier system, avoiding the loss and mutual interference of the reactant media of each layer, along with convenient assembly and disassembly, light weight and low cost;
the heights of the first clamping groove 11, the second clamping groove 12 and the third clamping groove 13 are all 20-35 cm, and the heights are favorable for clamping the first pervious concrete cavity 31, the second pervious concrete cavity 32 and the third pervious concrete cavity 33.
The shapes of the first clamping groove 11, the second clamping groove 12 and the third clamping groove 13 correspond to the bottom shapes of the first pervious concrete cavity 31, the second pervious concrete cavity 32 and the third pervious concrete cavity 33 respectively; the shapes of the first card slot 11, the second card slot 12 and the third card slot 13 are preferably U-shaped; the width of the clamping groove is determined by the width of the placed pervious concrete cavity;
the concrete top cover 12 is C30 common concrete, the thickness of the top cover is 10 cm-15 cm, and the inner width of the top cover is determined according to the total width of the permeable reactive barrier.
In a preferred embodiment, the preparation method of the mineralized layer of the bacillus phosphate solubilizing bacterium comprises the following steps:
obtaining the bacillus phosphate solubilizing bacteria, the phosphate rock powder and the first quartz sand, wherein the bacillus phosphate solubilizing bacteria is OD6001.5-3 of a bacillus phosphate solubilizing suspension;
and (3) mixing the bacillus phosphate solubilizing bacteria and the phosphate rock powder according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria;
uniformly mixing the phosphate rock powder loaded with the phosphate solubilizing bacillus and the first quartz sand to obtain a phosphate solubilizing bacillus mineralization layer; wherein the volume ratio of the phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria to the first quartz sand is 1: (1-1.5).
The reason why the phosphate rock powder is used as the phosphate solubilizing bacillus load body is as follows: 1) the ground phosphate rock has the adsorption property, and can be used for immobilizing bacteria on the surface of the ground phosphate rock; 2) the bacillus phosphate solubilizing bacteria can dissolve insoluble phosphate rock powder by secreting organic acid to provide phosphate ions for fixing heavy metals; 3) the ground phosphate rock also has better functions of adsorption, complexation and ion exchange on the heavy metal, and can further enhance the effect of removing the heavy metal from the mineralized layer of the bacillus phosphate solubilizing.
The reason for uniformly mixing the phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria and the first quartz sand is as follows: the quartz sand is used as coarse aggregate in the reaction medium due to the larger grain size of the quartz sand so as to ensure that the reaction medium has better permeability.
OD is selected from the bacillus phosphate solubilizing bacteria600The reason for the bacillus phosphate solubilizing suspension of 1.5-3 is: OD600The size is the absorbance value of the bacterial suspension at a wavelength of 600nm, the value size represents the concentration of the bacterial suspension, and the bacterial suspension with the concentration in the range is commonly used when the microorganism/bacteria is used for environmental treatment, and the value is selected to be larger in the range according to the larger concentration of the corresponding remediation pollutant.
The mass-volume ratio of the bacillus phosphate solubilizing bacteria to the phosphate rock powder is 1: the reasons for (0.8-1.2) are: the value is a better proportion found by experiments, if the ratio is too small, the number of the phosphorus-dissolving bacillus in the reaction medium is insufficient, and the repair reaction period is long; the effect is not improved due to the overlarge value, the cost is increased, and the cost performance is low.
The volume ratio of the phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria to the first quartz sand is 1: the reasons for (1-1.5) are: the value is a better proportion found by experiments, and is insufficient in reaction substances and poor in repairing effect when being too small; the repairing effect is not improved due to the overlarge value, and the cost performance is low; depending on the contaminant concentration, the higher the degree of contamination in this range, the larger the ratio.
In a preferred embodiment, the method for preparing the mineralized layer of anaerobic bacillus comprises the following steps:
obtaining the anaerobic bacillus, the first active carbon and the second quartz sand, wherein the anaerobic bacillus is OD6001.5-3 of an anaerobic bacillus suspension;
mixing the anaerobic bacillus and the first activated carbon according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain first activated carbon loaded with anaerobic bacillus;
uniformly mixing the second quartz sand and the first activated carbon loaded with the anaerobic bacillus to obtain an anaerobic bacillus mineralization layer; wherein the volume ratio of the first activated carbon loaded with anaerobic bacillus to the second quartz sand is 1: (1-1.5).
The anaerobic bacillus selects OD600The reason for the suspension of 1.5 to 3 anaerobic bacillus is: OD600 this value is the absorbance of the bacterial suspension at a wavelength of 600nm, the magnitude of which is representative of the bacterial suspension concentration, and is generally in this range or lower when treating the environment with microorganisms/bacteria, the value being selected to be greater in this range as the corresponding remediating contaminant concentration is greater.
The mass-volume ratio of the anaerobic bacillus to the first activated carbon is 1: the reasons for (0.8-1.2) are: the value is a better proportion found by experiments, if the ratio is too small, the microorganism content in the initial repair stage is insufficient, so that the initial repair effect is poor, and the microorganism content required for achieving the target effect after a period of propagation is needed; the excessive value does not improve the effect, increases the cost, has low cost performance,
The volume ratio of the second quartz sand to the first activated carbon is 1: the reasons for (1-1.5) are: the value is a better proportion found by experiments, and is too large, so that the reaction substances are insufficient, and the repairing effect is poor; if the value is too small, the effect is not improved, and the cost performance is not high; depending on the concentration of the contaminant, the higher the degree of contamination in this range, the smaller the ratio.
In a preferred embodiment, the preparation method of the aerobic bacillus oxygen reduction and explanation layer comprises the following steps:
obtaining the aerobic bacillus,The peroxide, the second activated carbon and the third quartz sand, and the aerobic bacillus is OD6001.5-3 of aerobic bacillus suspension;
mixing the aerobic bacillus and the second activated carbon according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain second activated carbon loaded with aerobic bacillus;
uniformly mixing the peroxide, the third quartz sand and the second activated carbon loaded with aerobic bacillus to obtain an aerobic bacillus mineralization layer; wherein the volume ratio of the third quartz sand to the peroxide to the aerobic bacillus-loaded second activated carbon is (1-1.5): (0.4-0.7): 1.
the mineralized layer of the bacillus phosphate solubilizing bacteria selects phosphate rock powder as a carrier; the reason why the activated carbon (instead of the rock phosphate powder) is selected as the carrier in the anaerobic bacillus degradation layer and the activated carbon (instead of the rock phosphate powder) is selected as the carrier in the aerobic bacillus degradation oxygen release layer is as follows: because the restoration objects of the anaerobic bacillus degradation layer and the aerobic bacillus degradation oxygen layer are organic pollutants, the biochar has better adsorption effect on the organic pollutants, the removal effect of the reaction layer on the organic pollutants can be further enhanced, and the ground phosphate rock can be used as a bacterial load body, but has no effect on the organic pollutants, and has no additional effect, so the biochar is selected.
The volume ratio of the third quartz sand to the peroxide mass to the second activated carbon is (1-1.5): (0.4-0.7): the reason for 1 is: the value is a better ratio found by experiments; the peroxide can be MgO2、CaO2Etc., which may react with water to release oxygen. If the peroxide is excessive, the excessive oxygen blocks the circulation of polluted underground water in the aerobic layer, so that the repair efficiency of the permeable reactive barrier is reduced. If the peroxide is too little and the oxygen yield is insufficient, the repair effect of the aerobic microorganisms in the layer is poor.
The volume ratio of the third quartz sand to the second activated carbon is 1: the reasons for (1-1.5) are: the value is a better proportion found by experiments, and is too large, so that the reaction substances are insufficient, and the repairing effect is poor; if the value is too small, the effect is not improved, and the cost performance is not high; depending on the concentration of the contaminant, the higher the degree of contamination in this range, the smaller the ratio.
In a preferred embodiment, the particle sizes of the first quartz sand, the second quartz sand and the third quartz sand are all 0.1mm to 3 mm; when the quartz sand in the range is used as the coarse aggregate of the reaction medium in the permeable reaction wall, the reaction medium has moderate water permeability, is larger than the soil body of the surrounding field, can guide the inflow of underground water, and simultaneously ensures that the polluted underground water is fully stayed and reacted in the reaction medium, so that the polluted underground water cannot flow away too fast.
The particle sizes of the first active carbon and the second active carbon are both 0.05 mm-1 mm. This range is selected as a preferable range found by experiments, and the adsorption effect of the activated carbon decreases as the particle size increases.
It should be noted that the particle size ranges of the first quartz sand, the second quartz sand, the third quartz sand, the first activated carbon and the second activated carbon are not intended to be a definite value within the range, but indicate that the first quartz sand, the second quartz sand, the third quartz sand, the first activated carbon or the second activated carbon may have different particle sizes, and only need to be within the range.
According to another exemplary embodiment of the present invention, there is provided a method for preparing a bio-permeable reactive barrier for a composite contaminated site, as shown in fig. 2, the method including:
s1, obtaining a concrete base and horizontally placing the concrete base;
s2, obtaining a reaction wall cavity, arranging a reaction area filler in the reaction wall cavity, and arranging the reaction wall cavity on the concrete base, wherein the reaction area filler comprises a phosphorus-dissolving bacillus mineralization layer, an anaerobic bacillus degradation layer and an aerobic bacillus degradation oxygen generation layer which are sequentially arranged along the groundwater seepage direction;
and S3, arranging a concrete top cover on the top of the cavity of the reaction wall.
Specifically, in S1, the concrete base is provided with the first slot 11, the second slot 12 and the third slot 13;
specifically, the reaction wall cavity in S2 includes a first pervious concrete cavity 31, a second pervious concrete cavity 32 and a third pervious concrete cavity 33; the first pervious concrete cavity 31, the second pervious concrete cavity 32 and the third pervious concrete cavity 33 are correspondingly arranged on the first clamping groove 11, the second clamping groove 12 and the third clamping groove 13 one by one.
The invention adopts the bacillus which is screened from the polluted site and takes organic pollutants as food as the reaction microorganism, the microorganism flora has strong adaptability to the polluted environment and high reaction activity, no additional nutrient substances are required to be added into the reaction wall, and the rapid loss of the reaction medium is avoided, so the reaction wall has long service life, in addition, the bacillus is taken as the reaction microorganism, the dormant characteristic of spore production is utilized, the elastic restoration can be realized aiming at the polluted site with variable pollution degree, and the standby prevention and control can be realized aiming at the high-risk sudden polluted site.
The biological permeable reactive barrier of a composite pollution site of the present application will be described in detail with reference to examples, comparative examples and experimental data.
S1, obtaining a concrete base 1, horizontally placing the concrete base 1, wherein the concrete base in the S1 is provided with a first clamping groove 11, a second clamping groove 12 and a third clamping groove 13;
s2, obtaining a reaction wall cavity 3, wherein the reaction wall cavity comprises a first pervious concrete cavity 31, a second pervious concrete cavity 32 and a third pervious concrete cavity 33; the wall thicknesses of the first, second and third pervious concrete cavities are shown in table 1.
Arranging a reaction area filler in the reaction wall cavity 3, arranging the reaction wall cavity 3 on the concrete base 1, wherein the reaction area filler 4 comprises a phosphorus-dissolving bacillus mineralization layer 41, an anaerobic bacillus degradation layer 42 and an aerobic bacillus degradation oxygen layer 43 which are sequentially arranged along the groundwater seepage direction; the phosphate solubilizing bacillus mineralization layer, the anaerobic bacillus degradation layer and the aerobic bacillus degradation oxygen layer are sequentially arranged in the first permeable concrete cavity, the second permeable concrete cavity and the third permeable concrete cavity.
The raw materials of the mineralized layer of the bacillus phosphate solubilizing comprise bacillus phosphate solubilizing, first quartz sand and phosphate rock powder;
the raw materials of the anaerobic bacillus degradation layer comprise anaerobic bacillus, second quartz sand and first activated carbon;
the raw materials of the aerobic bacillus degradation oxygen layer comprise aerobic bacillus, peroxide, third quartz sand and second activated carbon; the bacillus phosphate solubilizing bacteria, the anaerobic bacillus and the aerobic bacillus are screened from a polluted site to be repaired, and are domesticated to take organic pollutants as required carbon sources and nitrogen sources.
The thickness and the compactness of the phosphate solubilizing bacillus mineralization layer, the anaerobic bacillus degradation layer and the aerobic bacillus degradation oxygen layer are shown in table 2, and the material ratio among the raw materials is shown in table 3.
And S3, arranging a concrete top cover on the top of the cavity of the reaction wall.
The permeable reactive barrier in the embodiment 1 is applied to a heavy metal-organic composite polluted site which is prominently polluted by heavy metal and seriously polluted by heavy metal, the soil layer of the site is sandy soil, and the height of a cavity is determined to be 6m according to the underground hydrology condition;
the permeable reactive barrier in the embodiment 2 is applied to a heavy metal-organic composite polluted site which is prominently polluted by organic matters and seriously polluted, the polluted soil layer is sandy silt, and the height of a cavity is determined to be 5m according to the underground hydrology condition;
the permeable reactive barrier in the embodiment 3 is applied to a heavy metal-organic composite polluted site seriously polluted by heavy metal and organic matters, the polluted soil layer is silty sandy soil, and the height of the cavity is determined to be 4 m.
TABLE 1
TABLE 2
TABLE 3
The repairing effect of the permeable reactive barrier prepared in each example and each comparative example in the contaminated site is as follows, and the analysis results are shown in table 4.
TABLE 4
From the data in table 4, it can be seen that:
in comparative example 1, the mass-to-volume ratio of the bacillus to the carrier was 0.5: 1.2, which is smaller than the ratio of the invention, and the rest is the same as the example 1, the removal effect on Pb (II) is 73 percent, the removal effect on Cu (II) is 80 percent, the restoration rate on COD is only 69 percent, the restoration rate on BOD is only 85 percent, and the restoration effect is not good;
in comparative example 2, the ratio of the mass of the apatite ore powder attached with the phosphorus-dissolving bacillus to the volume of the first quartz sand, and the mass of the first activated carbon loaded with the anaerobic bacillus to the volume of the second quartz sand are all 0.5:1, which is smaller than the ratio of the present invention, and the rest of the same examples 1, the Pb (ii) removal effect is 74%, the COD restoration rate is only 71%, and the restoration effect is not good;
in comparative example 3, the ratio of the volume of the third silica sand, the amount of the peroxide, and the volume of the aerobic bacillus-loaded second activated carbon was 1.5: 0.3: 1, peroxide accounts for a small proportion of the invention, and the rest is the same as the embodiment 1, the repair rate to COD is only 75%, the repair rate to BOD is only 77%, and the repair effect is poor;
in comparative example 4, the thickness of the phosphate solubilizing bacillus mineralized layer, the anaerobic bacillus degraded layer and the aerobic bacillus degraded oxygen layer is 25cm and is less than the range of 30 cm-40 cm of the invention, the repair rate to Pb (II) is 83%, the repair rate to Zn (II) is 84%, the repair rate to Cu (II) is 79%, the repair rate to COD is only 78.7%, the repair rate to BOD is only 85%, and the repair effect is not good;
the bacillus microorganism permeable reactive barrier prepared in the embodiment 1-embodiment 3 has good repairing effect, the repairing rate of Pb (II), Zn (II), Cu (II), COD (chemical oxygen demand) and BOD (biochemical oxygen demand) is more than 99%, the purifying capacity is strong, and the service strength of a high-risk sudden pollution site can be flexibly adjusted according to the pollution degree.
In conclusion, the device consists of three layers of reaction media, namely a phosphorite dissolving layer for repairing heavy metals, an anaerobic layer for degrading organic pollutants and an aerobic layer, wherein the three layers are paved layer by layer and go forward, and the heavy metal-organic composite pollutants in the polluted underground water can be efficiently removed through the actions of mineralization, adsorption, biodegradation, oxidation reduction and the like; and the dormant characteristic of spore production of the bacillus is utilized, the requirement of prevention and control of a high-risk sudden pollution site on standby service of the permeable reactive barrier can be met, and the service strength of the pollution site with variable pollution degrees can be flexibly adjusted according to the pollution degrees.
The proportion of each component in the phosphorite dissolving layer, the anaerobic layer and the aerobic layer for degrading organic pollutants is further controlled, and the bacillus microorganism permeable reactive barrier with good performance can not be obtained if any component is lacked or the content is not in the range.
Finally, it should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The biological permeable reactive barrier for the composite contaminated site is characterized by comprising a concrete base, a concrete top cover, a reactive barrier cavity and reactive zone fillers, wherein the reactive barrier cavity is arranged between the concrete base and the concrete top cover, and the reactive zone fillers are arranged in the reactive barrier cavity;
the reaction area filler comprises a phosphorus-dissolving bacillus mineralization layer, an anaerobic bacillus degradation layer and an aerobic bacillus degradation oxygen layer which are sequentially arranged along the groundwater seepage direction;
the raw materials of the mineralized layer of the bacillus phosphate solubilizing comprise bacillus phosphate solubilizing, first quartz sand and phosphate rock powder;
the raw materials of the anaerobic bacillus degradation layer comprise anaerobic bacillus, second quartz sand and first activated carbon;
the raw materials of the aerobic bacillus degradation oxygen layer comprise aerobic bacillus, peroxide, third quartz sand and second activated carbon.
2. The biological permeable reactive barrier for the composite polluted site as claimed in claim 1, wherein the bacillus phosphate solubilizing bacteria, the anaerobic bacillus and the aerobic bacillus are domesticated to take organic pollutants as a required carbon source and a required nitrogen source; the phosphate solubilizing bacillus, the anaerobic bacillus and the aerobic bacillus are purchased bacteria or screened from polluted sites.
3. The biological permeable reactive barrier for a composite contaminated site according to claim 1, wherein the particle sizes of the first quartz sand, the second quartz sand and the third quartz sand are all 0.1mm to 3 mm; the particle sizes of the first active carbon and the second active carbon are both 0.05 mm-1 mm.
4. The biological permeable reactive barrier of a composite pollution site as claimed in claim 1, wherein the thickness of the mineralization layer of the phosphate solubilizing bacillus, the degradation layer of the anaerobic bacillus and the degradation layer of the aerobic bacillus are all 30 cm-40 cm.
5. The biological permeable reactive barrier of a composite pollution site according to claim 1, wherein the preparation method of the mineralized layer of the bacillus phosphate solubilizing bacterium is as follows:
obtaining the bacillus phosphate solubilizing bacteria, the phosphate rock powder and the first quartz sand, wherein the bacillus phosphate solubilizing bacteria is OD6001.5-3 of a bacillus phosphate solubilizing suspension;
and (3) mixing the bacillus phosphate solubilizing bacteria and the phosphate rock powder according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria;
uniformly mixing the phosphate rock powder loaded with the phosphate solubilizing bacillus and the first quartz sand to obtain a phosphate solubilizing bacillus mineralization layer; wherein the volume ratio of the phosphate rock powder loaded with the bacillus phosphate solubilizing bacteria to the first quartz sand is 1: (1-1.5).
6. The biological permeable reactive barrier for the composite polluted site according to claim 1, wherein the preparation method of the mineralized layer of the anaerobic bacillus comprises the following steps:
obtaining the anaerobic bacillus, the first active carbon and the second quartz sand, wherein the anaerobic bacillus is OD6001.5-3 of an anaerobic bacillus suspension;
mixing the anaerobic bacillus and the first activated carbon according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain first activated carbon loaded with anaerobic bacillus;
uniformly mixing the second quartz sand and the first activated carbon loaded with the anaerobic bacillus to obtain an anaerobic bacillus mineralization layer; wherein the volume ratio of the first activated carbon loaded with anaerobic bacillus to the second quartz sand is 1: (1-1.5).
7. The biological permeable reactive barrier for the composite polluted site according to claim 1, wherein the preparation method of the mineralized layer of aerobic bacillus comprises the following steps:
obtaining the aerobic bacillus, the peroxide, the second active carbon and the third quartz sand, wherein the aerobic bacillus is OD6001.5-3 of aerobic bacillus suspension;
mixing the aerobic bacillus and the second activated carbon according to a mass-volume ratio of 1: (0.8-1.2) carrying out uniform mixing and air drying to obtain second activated carbon loaded with aerobic bacillus;
uniformly mixing the peroxide, the third quartz sand and the second activated carbon loaded with aerobic bacillus to obtain an aerobic bacillus mineralization layer; wherein the volume ratio of the third quartz sand to the peroxide to the aerobic bacillus-loaded second activated carbon is (1-1.5): (0.4-0.7): 1.
8. the biological permeable reactive barrier for the composite contaminated site according to claim 1, wherein the reactive barrier cavity comprises a first permeable concrete cavity, a second permeable concrete cavity and a third permeable concrete cavity; the phosphate solubilizing bacillus mineralization layer, the anaerobic bacillus degradation layer and the aerobic bacillus degradation oxygen layer are sequentially arranged in the first permeable concrete cavity, the second permeable concrete cavity and the third permeable concrete cavity.
9. The biological permeable reactive barrier for the composite contaminated site according to claim 8, wherein the wall thickness of the first, second and third permeable concrete cavities is 15cm to 25 cm; the concrete foundation is provided with a clamping groove, the clamping groove comprises a first clamping groove, a second clamping groove and a third clamping groove, and the first water-permeable concrete cavity, the second water-permeable concrete cavity and the third water-permeable concrete cavity are arranged on the first clamping groove, the second clamping groove and the third clamping groove in a one-to-one correspondence mode.
10. A method for preparing a biological permeable reactive barrier of a composite pollution site according to any one of claims 1 to 9, wherein the method comprises the following steps:
obtaining a concrete base, and horizontally placing the concrete base;
obtaining a reaction wall cavity, arranging a reaction area filler in the reaction wall cavity, and arranging the reaction wall cavity on the concrete base, wherein the reaction area filler comprises a phosphorus-dissolving bacillus mineralization layer, an anaerobic bacillus degradation layer and an aerobic bacillus degradation oxygen layer which are sequentially arranged along the groundwater seepage direction;
and arranging a concrete top cover at the top of the reaction wall cavity.
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