CN110129208A - One plant of phosphorus decomposing penicillium oxalicum with wide spectrum characteristic of lactic acid production - Google Patents
One plant of phosphorus decomposing penicillium oxalicum with wide spectrum characteristic of lactic acid production Download PDFInfo
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- CN110129208A CN110129208A CN201910351010.2A CN201910351010A CN110129208A CN 110129208 A CN110129208 A CN 110129208A CN 201910351010 A CN201910351010 A CN 201910351010A CN 110129208 A CN110129208 A CN 110129208A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
Abstract
The invention discloses one plant of phosphorus decomposing penicillium oxalicums with wide spectrum characteristic of lactic acid production.The deposit number of the bacterial strain are as follows: CCTCC NO.M2019233 is well grown in the solid medium for being only phosphorus source with calcium phosphate or ferric phosphate, can be growth substance using insoluble phosphorus compound after culture 48 hours, and culture grew a large amount of bacterium colonies after 3-7 days；In liquid medium, calcium phosphate or ferric phosphate which is 50 g/L using maximum concentration, highest can produce the soluble phosphate of 2500 mg/L.The bacterial strain is in addition solid phosphoric acid calcium culture medium, metabolizable generation oxalic acid, formic acid, malic acid, acetic acid and lactic acid, and the maximum secreted is up to 215.73mg/L.Present invention microbial resources isolated for the first time from soil environment have good application value to improve agricultural land soil phosphorus element, increase soil fertility and promote soil pollution by heavy metal comprehensive treatment etc..
Description
Technical field
The invention belongs to field of environmental biotechnology, and in particular to one plant of phosphorus decomposing Fungal oxalate with wide spectrum characteristic of lactic acid production
Mould PSF-4 and its application.
Background technique
Phosphorus is one of a great number of elements necessary to biological growth and development, is composition biomembrane, nucleic acid and many macromoleculars
The important composition ingredient of (such as atriphos).P elements are the most important battalion for being only second to nitrogen for plant growth
Support element.Phosphorus is converted with different compound forms, is flowed between plant, animal, microorganism and soil, animal and
Microorganism can be by the P elements needed for supplementing the effects of predation or catabolism, and plant mainly passes through plant root reality
(main include primary phosphoric acid H to biological effective state phosphorus in existing soil2PO4 -With secondary phosphoric acid HPO4 2-) absorption.Therefore, in soil
The content of biological effective state phosphorus is phosphorus compound conversion and phosphorus in the important environmental factor and soil environment for influence plant growth
The main constituents of element circular.
Total phosphorus content in soil is very high, but 95% or more phosphorus is existed in the form of indissoluble state phosphorus compound, raw
Object available P content is lower, lacks phosphorus phenomenon so as to cause soil.It is reported that the whole world is in there are about 67% soil lacks phosphorus shape
State, there are about 74% arable soils to lack phosphorus in China.Though application inorganic phosphorus fertilizer can solve the problems, such as that plant lacks phosphorus in the short time, it increases
Agriculture production cost is added, while the heavy metal (such as lead, zinc) in phosphate fertilizer is easily introduced soil environment, leads to environmental pollution.
Phosphate solubilizing microorganism refers to can be by indissoluble state phosphorus compound (including organic phosphorus and inorganic phosphorous compound) in soil environment
It is converted to the microorganism of soluble state phosphorus, indissoluble state phosphorus compound can be driven to decompose or be dissolved as soluble state phosphorus element, is inhaled for plant
It receives and utilizes, to improve the conversion ratio and utilization rate of biological effective state phosphorus element in soil, while reducing soil phosphorus accumulation, and promote
It is grown in the soil for lacking phosphorus, pH value slant acidity into plant.Complete confirmation is not yet received in the phosphorus decomposing mechanism of phosphate solubilizing microorganism at present,
But recent studies indicate that, the dissolution of indissoluble state phosphorus compound is related to phosphate solubilizing microorganism secretion organic acid, it is extracellular can
To dissolve the inorganic phosphorous compound of slightly solubility, such as Ca3(PO4)2、FePO4Deng thus by H therein2PO4 -、HPO4 2-Etc. solubilities
Phosphate radical release.Simultaneously in recent years the study found that phosphate solubilizing bacteria secretion a variety of organic acids, be alternatively arranged as good life
Object chelating agent is used for heavy-metal contaminated soil, reduces the concentration of soil heavy metal ion, so that it is dirty to reach effective remediating heavy metal
The purpose of dye.However, the phosphorus decomposing fungi of the tool wide spectrum metabolism of organic acids characteristic obtained both at home and abroad at present is less, therefore, to phosphorus decomposing
The research that bacterium is applied to soil remediation is also extremely limited.
Summary of the invention
In order to solve defect and deficiency of the existing technology, the present invention provides one plant of phosphorus decomposing with wide spectrum characteristic of lactic acid production
Microorganism penicillium oxalicum PSF-4, the bacterial strain have been sent to China typical culture collection center (CCTCC) guarantor on April 4th, 2019
Hiding, deposit number are as follows: CCTCC NO:M2019233, classification naming: penicillium oxalicum PSF-4Penicillium oxalicum
PSF-4, address: the Chinese Wuhan Wuhan University.
Another object of the present invention is to provide the application of penicillium oxalicum PSF-4, bacterial strain tool provided by the invention is efficient
The fungi of phosphorus decomposing characteristic, while can secrete and generate a variety of organic acid compounds.The bacterial strain is in laboratory conditions in solid phosphorus
In sour calcium and ferric phosphate phosphorus decomposing culture medium, PSF-4 can be grown within 72h, and form significant transparent phosphorus decomposing circle.PSF-4
The efficiency of degradation calcium phosphate is higher than ferric phosphate；Meanwhile in the experiment of solid phosphoric acid calcium liquid shaking bottle, PSF-4 be can produce largely
Organic acid finds 5 kinds of organic acids such as metabolizable generation oxalic acid, formic acid, malic acid, acetic acid and lactic acid through HPLC detection.Improving
Agricultural land soil phosphorus element increases soil fertility and promotes soil pollution by heavy metal comprehensive treatment etc. with important before
Scape.
In order to achieve the above-mentioned object of the invention, The technical solution adopted by the invention is as follows:
In a first aspect, providing one plant of penicillium oxalicum PSF-4, which is sent to Chinese Typical Representative culture on April 4th, 2019
Object collection (CCTCC) preservation, deposit number are as follows: CCTCC NO:M2019233, classification naming: penicillium oxalicum PSF-
4Penicillium oxalicum PSF-4, address: the Chinese Wuhan Wuhan University.Penicillium oxalicum PSF-4Penicillium
Oxalicum PSF-4 CCTCC NO:M2019233 abbreviation penicillium oxalicum PSF-4.
Second aspect provides a kind of microbial inoculum, which contains penicillium oxalicum PSF-4 Penicillium oxalicum
PSF-4, deposit number are as follows: CCTCC NO:M2019233.
Growth cycle of the bacterium in liquid and solid phosphorus decomposing culture medium is longer.Liquid fermentation medium shaking table shaken cultivation
Reach logarithmic growth phase in 48 hours, phosphorus decomposing culture medium is become clarifying from muddiness.On phosphorus decomposing solid medium, fungus colony is big
Small irregular, edge is white fluffy, and the aobvious green in mature bacterium colony center, aerial hyphae and Ji Sheng mycelia are flourishing, easy picking.Bacterium
It falls as irregular cycle, flat protuberance is easily provoked；Bacterium colony is larger, and edge is irregular.It observes under an optical microscope, bacterium colony is in
Velvet-like, branch bifurcated, mostly three is forked, center asymmetrical type bifurcated；Spore color is green, and shape is ellipse, spherical, directly
Diameter is about 0.10 μm；Sporophore is short and thick, and branch is obvious, and forked acute angle branch, branch is not symmetrical, and length is about 0.25 μm.Bacterium
The ITS rDNA gene order of strain has carried out sequencing analysis, and sequence has been filed on to GenBank database, passes through sequence alignment point
Analysis, the dientification of bacteria are penicillium oxalicum (Penicillium oxalicum), Serial No. MK720103 in GenBank.
In solid phosphoric acid calcium and ferric phosphate phosphorus decomposing culture medium, PSF-4 can be grown penicillium oxalicum PSF-4 within 72h,
And form significant transparent phosphorus decomposing circle.In the liquid phosphorus decomposing culture medium that solid phosphoric acid calcium and phosphoric acid concentration of iron are 5-50g/L,
PSF-4 can be grown, and can degradable calcium phosphate and ferric phosphate under 5g/L concentration.The efficiency of PSF-4 degradation calcium phosphate
Higher than ferric phosphate, highest amount of phosphorus dissolved is more than 2400mg/L.By changing the experiment item such as cultivation temperature, fungi inoculum density, revolving speed
Part, PSF-4 are less to the degradation efficiency variation of solid phosphoric acid calcium, and 100% degradation can be achieved in highest, and maximum amount of phosphorus dissolved is more than
3000mg/L。
In the experiment of solid phosphoric acid calcium liquid shaking bottle, PSF-4 can produce a large amount of organic acid, can generation through HPLC detection discovery
It thanks and generates 5 kinds of organic acids such as oxalic acid, formic acid, malic acid, acetic acid and lactic acid, the secretion of organic acid enables to a part of in soil
Insoluble phosphate dissolution, to discharge the available phosphorus of bioavailable, phosphorus content, and the maximum secreted can be utilized by improving soil
It measures up to 215.7mg/L.
The third aspect provides the application of above-mentioned penicillium oxalicum PSF-4 or microbial inoculum in degradation slightly solubility inorganic phosphate.
Preferably, the inorganic phosphate is calcium phosphate or ferric phosphate, preferably calcium phosphate.
Fourth aspect provides above-mentioned penicillium oxalicum PSF-4 or microbial inoculum in the product of preparation degradation slightly solubility inorganic phosphate
In application.
5th aspect provides a kind of method for improving agricultural land soil phosphorus element, to use above-mentioned penicillium oxalicum PSF-4 or microbial inoculum
Soil is handled, thus slightly solubility inorganic phosphate in soil of degrading.
Bacterial strain of the present invention most common insoluble phosphorus compound in soil: calcium phosphate and ferric phosphate are only phosphorus source
Solid medium in well grow, after 48 hours of incubation can using insoluble phosphorus compound be growth substance, cultivate 3-7
A large amount of biological bacterium colonies are grown after it.In liquid medium, the phosphoric acid that bacterium strain capable of high-efficiency of the present invention is 50g/L using maximum concentration
Calcium and ferric phosphate, highest can produce the soluble phosphate of 2500mg/L.Invention also discloses the wide spectrum organic acid generations of fungi
Thank to characteristic, in addition solid phosphoric acid calcium culture medium, 5 kinds of metabolizable generation oxalic acid, formic acid, malic acid, acetic acid and lactic acid etc. has
Machine acid, and the maximum secreted is up to 215.7mg/L.Due to the solution of the tool wide spectrum metabolism of organic acids characteristic obtained both at home and abroad at present
Phosphorus fungi is less, and bacterial strain of the present invention is inventor's microbial resources isolated for the first time from soil environment, to improvement
Agricultural land soil phosphorus element increases soil fertility and promotes soil pollution by heavy metal comprehensive treatment etc., has good application
Value.
Detailed description of the invention
Fig. 1: the fungi PSF-4 growing state on calcium phosphate and ferric phosphate solid phosphorus decomposing plate；
A is calcium phosphate solid phosphorus decomposing plate, and B is ferric phosphate solid phosphorus decomposing plate；
Fig. 2: fungi PSF-4 sporophore and its molecular biology phylogenetic tree under an optical microscope；
A is the sporophore of fungi PSF-4 under an optical microscope, and B is fungi PSF-4 molecular biology phylogenetic tree；
The wide spectrum metabolism of organic acids characteristic of Fig. 3: HPLC detection PSF-4；
For the biological characteristics explanation of preservation fungi:
The fungi is preserved in China typical culture collection center, and deposit number is CCTCC NO:M2019233.Address: in
The Wuhan state Wuhan University, Classification system: Penicillium oxalicum PSF-4, Chinese are as follows: penicillium oxalicum PSF-
4, the bacterium was in preservation on April 4 in 2019.
Specific embodiment
By following detailed description combination attached drawing it will be further appreciated that the features and advantages of the invention.Provided implementation
Example is only the explanation to the method for the present invention, remaining content without limiting the invention in any way announcement.
The screening of [implementing 1] phosphorus decomposing fungi
It is natural immediately after acquisition in the activated sludge for testing soil sample Hunan Yueyang Yunxi District industry park sewage treatment plant
48h is air-dried, after removing undesired impurities, ground 20 mesh soil sieve, extra soil sample is placed in 4 DEG C of refrigerators and saves.Take 10g sieving soil
Sample, be added 100mL sterilizing liquid enriched medium (contain sterile glass beads) (enrichment culture based component: peptone 10g/L,
NaCl 1.0g/L, KH2PO31.0g/L, pH=7), 150rpm vibrates 7 days.After cultivating 1 week, the culture in enriched medium is taken
Liquid 1mL takes 1mL supernatant to be diluted to 10 according to dilution spread flat band method-5, take wherein 10-4,10-5,10-63 dilution gradients
Under 200 μ L of solution, taking respectively calcium phosphate and ferric phosphate as the phosphorus decomposing culture medium (ingredient of phosphorus decomposing culture medium of only phosphorus source
Are as follows: sucrose 10g/L, Ca3(PO4)2Or FePO410g/L, NaCl 0.3g/L, KCl 0.3g/L, MgS04·7H20 0.3g/L,
FeS04 0.03g/L,MnS04 0.03g/L,(NH4)2S040.5g/L, yeast extract 0.5g/L, phosphorus decomposing fluid nutrient medium
Ingredient and solid phase are same, but are free from agar) on be coated, each processing repeats 3 plates, and plate is then placed in 28 DEG C
Insulating box culture records the bacterial strain quantity on each plate, and observes the colony characteristics of microorganism on solid plate, root after 5 days
According to the phosphorus decomposing circle that bacterial strain each on phosphorus decomposing culture medium is formed, isolated purebred phosphate solubilizing microorganism of crossing.
According to Fig. 1 growing state can be seen that PSF-4 can in normal growth on the phosphorus decomposing plate of calcium phosphate and ferric phosphate,
And there is very high degradation capability.
The identification of [embodiment 2] bacterial strain
By the strain inoculated of purifying in solid PDA medium (ingredient: potato 200g/L, glucose 20g/L, agar 20g/
L, pH=7) colony morphology characteristic of the observation bacterial strain on solid plate after culture 5 days, then a little bacterium colony of picking is aobvious in optics
The microstructure of micro- microscopic observation bacterial strain, and record the morphological feature of bacterial strain: bacterium colony is in velvet-like, and branch bifurcated, mostly three is forked,
Center asymmetrical type bifurcated；Spore color is green, and shape is ellipse, and spherical, diameter is about 0.10 μm；Sporophore is short and thick,
Branch is obvious, and forked acute angle branch, branch is not symmetrical, and length is about 0.25 μm.
Fungi ITS rDNA sequencing includes that bacterial strain DNA is extracted, ITS rDNA amplification in vitro (uses ITS universal primer
ITS1 and ITS4) and gene sequencing.The sequence results being sequenced by genome company by software analysis, merge and bring into
The comparison of BLAST gene order is carried out in the database of NCBI.According to fig. 2 the phylogenetic tree of morphologic observation and building as a result,
The bacterial strain is determined as penicillium oxalicum (Penicillium oxalicum)
[embodiment 3] bacterial strain analyzes the degradation characteristic of calcium phosphate, ferric phosphate
Using liquid phosphorus decomposing culture medium, the final concentration after calcium phosphate, ferric phosphate is added is respectively set as 5g/L and 2g/L.It surveys
Before fixed, this strain inoculated is cultivated in phosphorus decomposing slant medium, 28 DEG C activation culture 72 hours, then take 1mL sterile water, add
Enter in inclined-plane, the spore of inclined-plane fungi and mycelia are scraped and form spore suspension in sterile water, is added to 5% volume ratio
In culture medium.All cultures are based on 28 DEG C of shaking table 150rpm revolving speed shaking table shaken cultivation 14 days, measure Medium's PH Value respectively
And titanium pigment content.Finally, PSF-4 is 2071mg/L to the amount of phosphorus dissolved that 5g/L calcium phosphate generates, and Soluble phosphorus rate is 100%, right
The amount of phosphorus dissolved that 2g/L ferric phosphate generates is 198mg/L, and Soluble phosphorus rate is 48.5%.
Use the content of titanium pigment in phosphorus antimony molybdenum blue spectrophotometric method detection supernatant.KH is used first2PO4Draw phosphorus
Standard curve, the degree of fitting for drawing curve should be more than 0.995.In measurement experiment group when titanium pigment content, liquid relief is used daily
Rifle accurately draws 50 μ L supernatants, is added in the centrifuge tube equipped with 950 μ L distilled water, and then 12000rpm is centrifuged 1min, small
The heart supernatant in centrifuge tube is added in test tube with a scale, then plus the anti-reagent of molybdenum antimony of 4mL and is settled to 25mL, room temperature is quiet
It sets sample to be placed at 700nm after 30min and measure, titanium pigment content is finally found out according to standard curve value, and calculate degradation
Characteristic.
Design 5,10,20,25, the calcium phosphate of 50g/L and 1,2,4,5,10g/L ferric phosphate are to study the degradation of PSF-4
Furthermore ability separately designs 24,28,32 DEG C of temperature gradients, design 10 using 5g/L calcium phosphate, 2g/L ferric phosphate as concentration of substrate6、
107、108Initial inoculation spore concentration, 100,150,200rpm revolving speed to be to study Decomposing phosphate activity.
Table 1: phosphorus decomposing fungi PSF-4 studies calcium phosphate and ferric phosphate degradation characteristic
It observes to obtain by 1 data of table and experimental phenomena, PSF-4 is very strong to the degradation capability of calcium phosphate, and highest can be real
Now it is more than the degradation concentration of 50g/L, and degradation rate is more than 19%, the complete drop to calcium phosphate can be achieved under low consistency conditions
Solution, and under varying experimental conditions, it can be achieved to the degradable of 5g/L calcium phosphate；To the degradation capability of ferric phosphate compared with
It is weak, the concentration of degradable 1g/L can only be realized within culture 14 days, and condition of culture variation is to the degradation efficiency of ferric phosphate
It is affected.
The detection and analysis of [embodiment 4] PSF-4 metabolism organic acid
Using high performance liquid chromatography (Agilent 1260Infinity) to calcium phosphate (5g/L, 10 being added before7/mL、
150rpm), ferric phosphate (2g/L, 107/ mL, 150rpm) fluid nutrient medium carry out organic acid detection and analysis, extract a certain amount of training
Base fluid body is supported, is centrifuged 2min in 12000rpm, then collect in liquid phase bottle by 0.22 μm of filter, the same day detects wherein organic acid
Content.Detector is UV detector, chromatographic column be enlightening horse platisil ODS C18 chromatographic column (4.6mm × 250mm, 5 μm,
Platisil company, the U.S.).Mobile phase: A phase is 0.01mol/L, and pH=2.9 ammonium dihydrogen phosphate, B phase is acetonitrile.Sample introduction
Amount: 20 μ L, column temperature: 30 DEG C, flow velocity: 0.5mL/min.A variety of organic acid standard curves are drawn first, draw the degree of fitting of curve
More than 0.995, when measuring organic acid concentration, sample is first passed through into 0.22 μm of filter membrane, then upper machine testing, by comparing sample peak
Relationship between area and standard curve can find out detected organic acid concentration.
Table 2: phosphorus decomposing fungi PSF-4 is to calcium phosphate and ferric phosphate secretion organic acid content research
Table 3: phosphorus decomposing fungi PSF-4 is to calcium phosphate secretion organic acid content research in one week
Claims (7)
1. one plant of penicillium oxalicum PSF-4, which is characterized in that strain classification name: penicillium oxalicum PSF-4Penicillium oxalicumPSF-4 has been preserved in China typical culture collection center, preservation date are as follows: on April 4th, 2019, preservation are compiled
Number are as follows: CCTCC NO:M2019233, preservation address are as follows: the Chinese Wuhan Wuhan University.
2. a kind of microbial inoculum, which is characterized in that the microbial inoculum contains penicillium oxalicum PSF-4 described in claim 1Penicillium oxalicum PSF-4。
3. penicillium oxalicum PSF-4 described in claim 1 or microbial inoculum as claimed in claim 2 are in degradation slightly solubility inorganic phosphate
In application.
4. application according to claim 3, which is characterized in that the slightly solubility inorganic phosphate is calcium phosphate or phosphoric acid
Iron.
5. application according to claim 4, which is characterized in that the slightly solubility inorganic phosphate is calcium phosphate.
6. penicillium oxalicum PSF-4 described in claim 1 or microbial inoculum as claimed in claim 2 are in preparation degradation slightly solubility Phos
Application in the product of hydrochlorate.
7. a kind of method for improving agricultural land soil phosphorus element, which is characterized in that the method is to use grass described in claim 1
Sour mould PSF-4 or microbial inoculum as claimed in claim 2 handle soil, thus slightly solubility inorganic phosphate in soil of degrading.
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Cited By (7)
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| CN110951625A (en) * | 2019-12-27 | 2020-04-03 | 滨州学院 | Penicillium phosphate solubilizing PSF and application thereof |
| CN111924982A (en) * | 2020-08-12 | 2020-11-13 | 中国科学院武汉岩土力学研究所 | Biological permeable reactive barrier for composite contaminated site and preparation method thereof |
| CN111979159A (en) * | 2020-09-03 | 2020-11-24 | 中南大学 | Phosphate solubilizing bacterium agent and preparation method and application thereof |
| CN113333459A (en) * | 2021-06-08 | 2021-09-03 | 湘潭大学 | Solid fermentation of penicillium oxalicum and method for efficiently dissolving phosphorus and removing lead by using same |
| CN114540208A (en) * | 2022-03-28 | 2022-05-27 | 山东省林业科学研究院 | Absidia capable of efficiently dissolving phosphorus and application thereof |
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| CN116410870A (en) * | 2023-02-14 | 2023-07-11 | 安徽农业大学 | Penicillium oxalate, composite microbial inoculum, combined repairing agent and application thereof |
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| CN111924982A (en) * | 2020-08-12 | 2020-11-13 | 中国科学院武汉岩土力学研究所 | Biological permeable reactive barrier for composite contaminated site and preparation method thereof |
| CN111979159A (en) * | 2020-09-03 | 2020-11-24 | 中南大学 | Phosphate solubilizing bacterium agent and preparation method and application thereof |
| CN113333459A (en) * | 2021-06-08 | 2021-09-03 | 湘潭大学 | Solid fermentation of penicillium oxalicum and method for efficiently dissolving phosphorus and removing lead by using same |
| CN113333459B (en) * | 2021-06-08 | 2022-06-03 | 湘潭大学 | Solid fermentation of penicillium oxalicum and method for efficiently dissolving phosphorus and removing lead by using same |
| CN114854600A (en) * | 2022-03-21 | 2022-08-05 | 中国科学院南京土壤研究所 | Penicillium oxalicum and application thereof |
| CN114540208A (en) * | 2022-03-28 | 2022-05-27 | 山东省林业科学研究院 | Absidia capable of efficiently dissolving phosphorus and application thereof |
| CN114540208B (en) * | 2022-03-28 | 2023-06-20 | 山东省林业科学研究院 | Absidia with high-efficiency phosphate-dissolving effect and application thereof |
| CN116410870A (en) * | 2023-02-14 | 2023-07-11 | 安徽农业大学 | Penicillium oxalate, composite microbial inoculum, combined repairing agent and application thereof |
| CN116410870B (en) * | 2023-02-14 | 2024-02-27 | 安徽农业大学 | Penicillium oxalate, composite microbial inoculum, combined repairing agent and application thereof |
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