CN1119213A - Production of zinc gluconate by fermenting glucose using Aspergillus niger - Google Patents

Production of zinc gluconate by fermenting glucose using Aspergillus niger Download PDF

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Publication number
CN1119213A
CN1119213A CN94116330A CN94116330A CN1119213A CN 1119213 A CN1119213 A CN 1119213A CN 94116330 A CN94116330 A CN 94116330A CN 94116330 A CN94116330 A CN 94116330A CN 1119213 A CN1119213 A CN 1119213A
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China
Prior art keywords
fermentation
glucose
aspergillus niger
zinc gluconate
seed
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CN94116330A
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Chinese (zh)
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CN1053471C (en
Inventor
计亮年
杨惠英
刘建忠
翁丽萍
王能东
鲁统部
叶保辉
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Sun Yat Sen University
National Sun Yat Sen University
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National Sun Yat Sen University
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Priority to CN94116330A priority Critical patent/CN1053471C/en
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Abstract

The method for producing medical or edible zinc gluconate by fermenting glucose with Aspergillus niger IFFI 2230 in the presence of zinc compound includes culture, fermentation, removing thallus, decolouring, concentration, crystallizing and drying, and features high yield up to 85%, simple process, low cost and energy consumption, and less chemical post-treatment.

Description

Production of zinc gluconate by fermenting glucose using Aspergillus niger
The present invention relates to a kind of fermentation method for producing of Zinc Gluconate, particularly adopt the method for production of zinc gluconate by fermenting glucose using Aspergillus niger.
Zinc Gluconate is a kind of bulk drug and foodstuff additive of trophicity supplement trace zinc, its production method mainly contains two kinds: a kind of is fermentation-chemical method, with aspergillus niger strain glucose oxidase is become gluconic acid, add lime carbonate or, be replaced as Zinc Gluconate with zinc type ion exchange resin again with in the sodium hydroxide and generation calglucon or Sunmorl N 60S; Also calglucon and zinc sulfate reaction can be generated Zinc Gluconate, above-mentioned two kinds of approach, the former is 59.7% to the yield of calglucon, and the latter's results of laboratory yield is 95%, but both all are that reactions steps is many, technical process is long and complicated, thereby produces investment greatly, the cost height; Another kind of production method is a chemical method, be glucose oxidase to be become gluconic acid with catalytic oxidation, neutralize with zinc oxide again, its results of laboratory is for to reach 80% to the glucose yield, this method production technique is still complicated, and temperature of reaction higher (60～90 ℃), also has the catalyzer step of removing in the aftertreatment, increase production cost and facility investment, also brought environmental pollution.
The objective of the invention is to adopt one-step fermentation direct production Zinc Gluconate.
Method of the present invention is to adopt aspergillus niger IFFI2230 slant strains, under the zn cpds existence condition, through seed culture, fermentation, remove thalline, decolouring, concentrate, crystallization and drying and other steps, glucose fermentation is generated Zinc Gluconate.
The fermentation condition that Zinc Gluconate is produced is as follows: at sterilized 5～15% glucose, the 0.037%KH of containing 2PO 4, 0.055% urea, 0.015%MgSO 40.1%CaCO 3In the fermentation culture of 1.0～5.0% zn cpdss, insert cultured seed liquid with the inoculum size of 10% (W/W), at 25～32 ℃, vapour-liquid ratio is 2.87～3.67m 3/ m 3Ventilating fermentation is 51～56 hours under the min condition, finishes fermentation when residual sugar amount＜1%.
The required zn cpds of fermenting process can be ZnO, ZnCl 2Or ZnCo 3, under the above-mentioned fermentation condition, glucose content is 5～15% o'clock, required ZnO is 1～3%, ZnCl 2Be 1.7～5.0%, ZnCo 3Be 1.5～4.5%, zn cpds can also be Zn (OH) 2
The used seed liquor of fermenting is cultivated by following condition: at sterilized 5% glucose, 0.012% sal epsom, 0.02%KCl, the 0.015%KH of containing 2PO 4, 0.06%NH 4H 2PO 4, 0.2% peptone and 0.3% extractum carnis seed culture medium in add aspergillus niger IFFI2230 slant strains after several rings activation, cultivated 24 hours with the 200rpm shaking table in 30 ℃.
After finishing fermentation, filtering fermentation liquor removes thalline, and add gac again and boil altogether with decolouring, concentrating under reduced pressure then, crystallizing and drying promptly gets the gluconic acid zinc product.
Method of the present invention adopts aspergillus niger IFFI2230 slant strains at zn cpds existence condition bottom fermentation glucose direct production Zinc Gluconate, finish oxidizing reaction at normal temperatures and pressures, but one-step fermentation obtains product, laboratory and pilot-scale experiment show that yield calculates by glucose all can be up to 85% (not counting secondary and three mother liquors recovery).Present method has been simplified technology, shortened technical process greatly, has less investment, the advantage that energy consumption is low, cost is low, whole technology compares to original chemical method and fermentation-chemical method, has reduced the chemical after-treatment reason, avoid introducing any reagent, thereby reduced environmental pollution, also greatly reduce the toxicity of product, as medicine or food safety and reliability.
Below in conjunction with specific embodiment the inventive method is further described:
Embodiment 1:
1. culture of seed liquid: at sterilized 5% glucose, the 0.012%MgSO of containing 4, 0.02%kCl, 0.015%KH 2PO 4, 0.06%NH 4H 2PO 4, 0.2% peptone and 0.3% extractum carnis seed culture medium in, add activated aspergillus niger IFFI2230 slant strains, cultivated 24 hours at 30 ℃, 200rpm shaking table, seed liquor;
2. fermentation: sterilized 8% glucose, the 0.037%KH of containing of the 6.5L that packs in the 10L airlift fermentor 2PO 4, 0.055% urea, 0.015%MgSO 4, 0.1%CaO and 3.4%ZnCl 2Fermentation culture, the inoculum size with 10% inserts cultured seed liquid, is 2.87%m at 32 ℃, vapour-liquid ratio 3/ m 3Ventilating fermentation is 54 hours under the min condition. when being 0.8%, finishes residual sugar fermentation;
3. remove thalline: put jar and remove thalline with the filter bag filtering fermentating liquid;
4. decolouring: add 2% gac and boil and decoloured in 5 minutes, gac is removed in centrifugation again;
5. condensing crystal: in 55 ℃ be evaporated to original volume 1/5th, crystallisation by cooling;
6. dry: suction filtration, filtrate change over to down and continue in the batch fermentation liquid to concentrate, and crystallization washes with water, in 90 ℃ of dryings, gets the gluconic acid zinc product.
It is 51.1% that whole process yield connects glucose calculating, does not count secondary and three mother liquors recovery.
Embodiment 2:
In the fermentation culture contained glucose be 8.44%, ZnCl 2Be 3.4%, fermentation condition is 32 ℃, vapour-liquid ratio 3.67%m 3/ m 3.min, aerobic fermentation 54 hours, the residual sugar amount is 0.78%.
It is 78% that yield calculates by glucose.
Other is all with embodiment 1.
Embodiment 3:
Contained ZnO is 2% in the fermentation culture, and it is 85% that yield calculates by glucose.
Other is all with embodiment 2.
Embodiment 4:
Contained ZnCO in the fermentation culture 3Be 3.1%, it is 80% that yield calculates by glucose, and other is all with embodiment 2.
Embodiment 5:
Contained glucose is 15% in the fermentation culture, ZnCl 2Be 5.0%, fermentation condition is 30 ℃, vapour-liquid ratio 3.67%m 3/ m 3.min, aerobic fermentation 52 hours, residual sugar amount 0.7%, it is 80% that yield calculates by glucose, other is all with embodiment 1.
Embodiment 6:
Contain 5.0% glucose, 1.7%ZnCl in the fermentation culture 2, fermentation condition is 25 ℃, vapour-liquid ratio 3.67%m 3/ m 3Min, aerobic fermentation 46 hours, residual sugar amount 0.5%, it is 50.8% that yield calculates by glucose.Other is all with embodiment 1.

Claims (4)

1, a kind of method of production of zinc gluconate by fermenting glucose using Aspergillus niger, through seed culture, fermentation, remove thalline, decolouring, concentrate, crystallization and drying and other steps, glucose fermentation is generated Zinc Gluconate, it is characterized in that: adopt aspergillus niger IFFI2230 slant strains, under the following conditions fermentation: at sterilized 5～15% glucose, the 0.037%KH of containing 2PO 4, 0.055% urea, 0.015%MgSO 4, 0.1%CaCO 3In the fermentation culture of 1.0～5.0% zn cpdss, insert cultured seed liquid with the inoculum size of 10% (W/W), be 2.87～3.67M at 25～32 ℃, vapour-liquid ratio 3/ M 3Under the min condition, ventilating fermentation 51～56 hours finishes fermentation when residual sugar＜1%.
2, method according to claim 1, it is characterized in that said zn cpds is ZnO or ZnCl 3Or ZnCO 3, content range is respectively 1～3%, 1.7～5.0% and 1.5～4.5%.
3, method according to claim 1 is characterized in that glucose content is 8.5% in the said fermentation culture, and ZnO content 2%, vapour-liquid ratio are 3.67M 3/ M 3Min, leavening temperature are 32 ℃, and fermentation time is 54 hours, and the residual sugar amount is 0.78% when finishing fermentation.
4, method according to claim 1 is characterized in that the seed liquor of said seed liquor for cultivating by following condition: at sterilized 5% glucose, the 0.012%MgSO of containing 4, 0.02%KCl, 0.015%KH 2PO 4, 0.06%NH 4H 2PO 4, 0.2% peptone and 0.3% extractum carnis seed culture medium in add aspergillus niger IFFI2230 slant strains after several rings activation, at 30 ℃, cultivated 24 hours with the 200rpm shaking table.
CN94116330A 1994-09-18 1994-09-18 Production of zinc gluconate by fermenting glucose using Aspergillus niger Expired - Fee Related CN1053471C (en)

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CN94116330A CN1053471C (en) 1994-09-18 1994-09-18 Production of zinc gluconate by fermenting glucose using Aspergillus niger

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CN94116330A CN1053471C (en) 1994-09-18 1994-09-18 Production of zinc gluconate by fermenting glucose using Aspergillus niger

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CN1053471C CN1053471C (en) 2000-06-14

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1054161C (en) * 1997-12-17 2000-07-05 中山大学 Producing of magnesium (manganese) gluconate by aspergillus fermentation glucose
CN1087781C (en) * 1998-05-28 2002-07-17 济南三株药业有限公司 Bioconversion method of selenium and zinc by utilizing beneficial bacterium
CN100413957C (en) * 2006-09-21 2008-08-27 江苏省农业科学院 Apergillus niger strain and application thereof
CN102080106A (en) * 2010-12-09 2011-06-01 山东西王生化科技有限公司 Double enzyme method for preparing zinc gluconate
CN103483181A (en) * 2013-08-30 2014-01-01 洪军 Calcium zinc gluconate compound

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RO97130B1 (en) * 1986-12-29 1989-07-02 Institutul De Cercetari Chimico-Farmaceutice Process for obtaining the technical calcium gluconate
CN1100306A (en) * 1994-05-30 1995-03-22 吴秀清 Compound zinc gluconate capsules, tablets and buccal tablets and their preparing method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1054161C (en) * 1997-12-17 2000-07-05 中山大学 Producing of magnesium (manganese) gluconate by aspergillus fermentation glucose
CN1087781C (en) * 1998-05-28 2002-07-17 济南三株药业有限公司 Bioconversion method of selenium and zinc by utilizing beneficial bacterium
CN100413957C (en) * 2006-09-21 2008-08-27 江苏省农业科学院 Apergillus niger strain and application thereof
CN102080106A (en) * 2010-12-09 2011-06-01 山东西王生化科技有限公司 Double enzyme method for preparing zinc gluconate
CN102080106B (en) * 2010-12-09 2012-11-14 山东西王生化科技有限公司 Double enzyme method for preparing zinc gluconate
CN103483181A (en) * 2013-08-30 2014-01-01 洪军 Calcium zinc gluconate compound
CN103483181B (en) * 2013-08-30 2015-08-26 洪军 A kind of Calcium zinc gluconate compound

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