CN111904917A - Method for extracting bamboo leaf compounds - Google Patents
Method for extracting bamboo leaf compounds Download PDFInfo
- Publication number
- CN111904917A CN111904917A CN202010543283.XA CN202010543283A CN111904917A CN 111904917 A CN111904917 A CN 111904917A CN 202010543283 A CN202010543283 A CN 202010543283A CN 111904917 A CN111904917 A CN 111904917A
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- Prior art keywords
- resin column
- distilled water
- washing
- ethanol
- until
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- 235000017166 Bambusa arundinacea Nutrition 0.000 title claims abstract description 41
- 235000017491 Bambusa tulda Nutrition 0.000 title claims abstract description 41
- 241001330002 Bambuseae Species 0.000 title claims abstract description 41
- 235000015334 Phyllostachys viridis Nutrition 0.000 title claims abstract description 41
- 239000011425 bamboo Substances 0.000 title claims abstract description 41
- 150000001875 compounds Chemical class 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 title claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 38
- 239000011347 resin Substances 0.000 claims abstract description 36
- 229920005989 resin Polymers 0.000 claims abstract description 36
- 238000005406 washing Methods 0.000 claims abstract description 23
- 239000012153 distilled water Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 12
- 239000012535 impurity Substances 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 12
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 230000007935 neutral effect Effects 0.000 claims abstract description 8
- 238000000746 purification Methods 0.000 claims abstract description 7
- 238000003809 water extraction Methods 0.000 claims abstract description 6
- 230000002378 acidificating effect Effects 0.000 claims abstract description 4
- 239000003463 adsorbent Substances 0.000 claims abstract description 4
- 238000001556 precipitation Methods 0.000 claims abstract description 4
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000032683 aging Effects 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 238000002481 ethanol extraction Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 239000002398 materia medica Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 150000004056 anthraquinones Chemical class 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0208—Tissues; Wipes; Patches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
The invention relates to an extraction method of bamboo leaf compounds, which comprises the following steps: bamboo leaf pretreatment, ultrasonic water extraction, alcohol precipitation and AB-8 pretreatment: soaking AB-8 in 95% ethanol for 24h to make AB-8 fully swell, removing impurities, and repeatedly washing resin column with distilled water at flow rate of 2 BV.h < -1 > until no ethanol residue exists; soaking and washing the resin column by using 5 percent HCl to remove acidic impurities, and repeatedly washing the resin column by using distilled water at the flow rate of 2 BV.h < -1 > until the resin column is neutral; soaking and washing the resin column with 2% NaOH to remove alkaline impurities, repeatedly washing the resin column with distilled water at a flow rate of 2 BV.h < -1 > until the resin column is neutral, and storing the resin in distilled water for later use; and (3) purification: purifying with activated AB-8 type macroporous adsorbent resin to obtain folium Bambusae extract.
Description
Technical Field
The invention relates to the field of compound extraction, in particular to an extraction method of bamboo leaf compounds.
Background
Bamboo leaves, sweet and bland in flavor, cold in nature, enter heart, lung and stomach meridians. The descriptions are in the books of materia medica, Anhui Chinese herbal medicine, dietetic therapy materia medica, etc. Bamboo leaves are favored by researchers in recent years because of the effects of clearing heat, relieving restlessness, promoting the production of body fluid, promoting urination, and being non-toxic and harmless. The bamboo leaf contains a large amount of active ingredients including flavonoids, phenolic acids, polysaccharides, anthraquinones, mineral elements, etc. Has antibacterial, antiseptic, antiinflammatory, antioxidant, and blood lipid reducing effects. Bamboo leaf extract is also gradually applied to food and health care product industries due to its characteristics of rich source, high safety, fresh taste and the like. Therefore, the bamboo leaf extract is added into the formula of the wet tissue, and has the effects of preventing corrosion and sterilizing, cooling and relieving and delaying senescence. However, the bamboo leaf compound has low activity, immature extraction technology and poor purification effect.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects in the prior art and provides an extraction method of bamboo leaf compounds.
The invention is realized by the following technical scheme:
a method for extracting bamboo leaf compounds comprises the following steps:
(1) bamboo leaf pretreatment: selecting a plurality of bamboo leaves with the length of 10-13 cm and the width of 1-2 cm, cleaning the bamboo leaves with tap water, washing the bamboo leaves with distilled water for 3 times, placing the cleaned bamboo leaves in a cool and ventilated place for airing, and then placing the bamboo leaves in a 70-DEG oven for drying to constant weight. Pulverizing, and sieving with 80 mesh sieve;
(2) ultrasonic water extraction: weighing a certain amount of pretreated bamboo leaf powder, and mixing the raw materials in a ratio of 1: 15-1: 25(g/mL) of distilled water is added and placed in a triangular flask, ultrasonic extraction is carried out for 10-60 minutes at 40-70 ℃ and under the power of 100-300W, suction filtration is carried out, re-extraction is carried out once again, and filtrate is combined for later use;
(3) alcohol precipitation: concentrating the filtrate to 80-120 mL, slowly adding a 95% ethanol solution with the volume 2-4 times that of the concentrated solution, stirring while adding ethanol until the content of ethanol in a solution system is 70-80%, settling at room temperature, centrifuging, and collecting precipitate;
(4) AB-8 pretreatment:
a: soaking AB-8 in 95% ethanol for 24h to make AB-8 fully swell, removing impurities, and repeatedly washing resin column with distilled water at flow rate of 2 BV.h < -1 > until no ethanol residue exists;
b: soaking and washing the resin column by using 5 percent HCl to remove acidic impurities, and repeatedly washing the resin column by using distilled water at the flow rate of 2 BV.h < -1 > until the resin column is neutral;
c: soaking and washing the resin column with 2% NaOH to remove alkaline impurities, repeatedly washing the resin column with distilled water at a flow rate of 2 BV.h < -1 > until the resin column is neutral, and storing the resin in distilled water for later use;
(5) and (3) purification: purifying with activated AB-8 type macroporous adsorbent resin to obtain folium Bambusae extract.
The invention has the beneficial effects that:
in order to fully extract and utilize the effective active ingredients in the bamboo leaves, the bamboo leaf extraction method has the following advantages: 1. the method is beneficial to separation and purification of water-soluble active ingredients, 2, the potential problem of ethanol residue in the traditional ethanol extraction method or water extraction and alcohol aging method is reduced, natural ingredients in the bamboo leaves can be extracted to a greater extent, and 3, the method meets the requirement of large-scale production.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the following preferred embodiments.
The invention comprises the following steps:
(1) bamboo leaf pretreatment: selecting a plurality of bamboo leaves with the length of 10-13 cm and the width of 1-2 cm, cleaning the bamboo leaves with tap water, washing the bamboo leaves with distilled water for 3 times, placing the cleaned bamboo leaves in a cool and ventilated place for airing, and then placing the bamboo leaves in a 70-DEG oven for drying to constant weight. Pulverizing, and sieving with 80 mesh sieve;
(2) ultrasonic water extraction: weighing a certain amount of pretreated bamboo leaf powder, and mixing the raw materials in a ratio of 1: 15-1: 25(g/mL) of distilled water is added and placed in a triangular flask, ultrasonic extraction is carried out for 10-60 minutes at 40-70 ℃ and under the power of 100-300W, suction filtration is carried out, re-extraction is carried out once again, and filtrate is combined for later use;
(3) alcohol precipitation: concentrating the filtrate to 80-120 mL, slowly adding a 95% ethanol solution with the volume 2-4 times that of the concentrated solution, stirring while adding ethanol until the content of ethanol in a solution system is 70-80%, settling at room temperature, centrifuging, and collecting precipitate;
(4) AB-8 pretreatment:
a: soaking AB-8 in 95% ethanol for 24h to make AB-8 fully swell, removing impurities, and repeatedly washing resin column with distilled water at flow rate of 2 BV.h < -1 > until no ethanol residue exists;
b: soaking and washing the resin column by using 5 percent HCl to remove acidic impurities, and repeatedly washing the resin column by using distilled water at the flow rate of 2 BV.h < -1 > until the resin column is neutral;
c: soaking and washing the resin column with 2% NaOH to remove alkaline impurities, repeatedly washing the resin column with distilled water at a flow rate of 2 BV.h < -1 > until the resin column is neutral, and storing the resin in distilled water for later use;
(5) and (3) purification: purifying with activated AB-8 type macroporous adsorbent resin to obtain folium Bambusae extract.
The invention has the beneficial effects that:
in order to fully extract and utilize the effective active ingredients in the bamboo leaves, the bamboo leaf extraction method has the following advantages: 1. the method is beneficial to separation and purification of water-soluble active ingredients, 2, the potential problem of ethanol residue in the traditional ethanol extraction method or water extraction and alcohol aging method is reduced, natural ingredients in the bamboo leaves can be extracted to a greater extent, and 3, the method meets the requirement of large-scale production.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (1)
1. A method for extracting bamboo leaf compounds is characterized by comprising the following steps: the method comprises the following steps:
(1) bamboo leaf pretreatment: selecting a plurality of bamboo leaves with the length of 10-13 cm and the width of 1-2 cm, cleaning the bamboo leaves with tap water, washing the bamboo leaves with distilled water for 3 times, placing the cleaned bamboo leaves in a cool and ventilated place for airing, and then placing the bamboo leaves in a 70-DEG oven for drying to constant weight. Pulverizing, and sieving with 80 mesh sieve;
(2) ultrasonic water extraction: weighing a certain amount of pretreated bamboo leaf powder, and mixing the raw materials in a ratio of 1: 15-1: 25(g/mL) of distilled water is added and placed in a triangular flask, ultrasonic extraction is carried out for 10-60 minutes at 40-70 ℃ and under the power of 100-300W, suction filtration is carried out, re-extraction is carried out once again, and filtrate is combined for later use;
(3) alcohol precipitation: concentrating the filtrate to 80-120 mL, slowly adding a 95% ethanol solution with the volume 2-4 times that of the concentrated solution, stirring while adding ethanol until the content of ethanol in a solution system is 70-80%, settling at room temperature, centrifuging, and collecting precipitate;
(4) AB-8 pretreatment:
a: soaking AB-8 in 95% ethanol for 24h to make AB-8 fully swell, removing impurities, and repeatedly washing resin column with distilled water at flow rate of 2 BV.h < -1 > until no ethanol residue exists;
b: soaking and washing the resin column by using 5 percent HCl to remove acidic impurities, and repeatedly washing the resin column by using distilled water at the flow rate of 2 BV.h < -1 > until the resin column is neutral;
c: soaking and washing the resin column with 2% NaOH to remove alkaline impurities, repeatedly washing the resin column with distilled water at a flow rate of 2 BV.h < -1 > until the resin column is neutral, and storing the resin in distilled water for later use;
(5) and (3) purification: purifying with activated AB-8 type macroporous adsorbent resin to obtain folium Bambusae extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010543283.XA CN111904917A (en) | 2020-06-15 | 2020-06-15 | Method for extracting bamboo leaf compounds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010543283.XA CN111904917A (en) | 2020-06-15 | 2020-06-15 | Method for extracting bamboo leaf compounds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111904917A true CN111904917A (en) | 2020-11-10 |
Family
ID=73237646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010543283.XA Pending CN111904917A (en) | 2020-06-15 | 2020-06-15 | Method for extracting bamboo leaf compounds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111904917A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1911101A (en) * | 2006-08-25 | 2007-02-14 | 胡林福 | Prodn. of antioxidant by extracting from bamboo leaves |
| CN101797447A (en) * | 2010-03-30 | 2010-08-11 | 武汉工程大学 | Method for separating and purifying effective ingredients in natural product |
| CN101889594A (en) * | 2010-07-06 | 2010-11-24 | 江南大学 | Method for extracting bacteriostatic agent from bamboo leaves with water |
-
2020
- 2020-06-15 CN CN202010543283.XA patent/CN111904917A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1911101A (en) * | 2006-08-25 | 2007-02-14 | 胡林福 | Prodn. of antioxidant by extracting from bamboo leaves |
| CN101797447A (en) * | 2010-03-30 | 2010-08-11 | 武汉工程大学 | Method for separating and purifying effective ingredients in natural product |
| CN101889594A (en) * | 2010-07-06 | 2010-11-24 | 江南大学 | Method for extracting bacteriostatic agent from bamboo leaves with water |
Non-Patent Citations (1)
| Title |
|---|
| 曹亚兰等: "提取纯化制备竹叶黄酮的研究", 《现代食品》 * |
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| PB01 | Publication | ||
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Application publication date: 20201110 |