CN111869364A - Method for promoting germination of chicory seeds - Google Patents
Method for promoting germination of chicory seeds Download PDFInfo
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- CN111869364A CN111869364A CN202010785869.7A CN202010785869A CN111869364A CN 111869364 A CN111869364 A CN 111869364A CN 202010785869 A CN202010785869 A CN 202010785869A CN 111869364 A CN111869364 A CN 111869364A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a method for promoting the germination of chicory seeds, which specifically comprises the following steps: seed soaking: firstly, soaking the seeds of the chicory in distilled water for 1-5 hours, and then soaking the seeds of the chicory; culturing: placing the soaked chicory seeds in a culture dish for culture, and placing two layers of filter paper in the culture dish; temperature change treatment: culturing the chicory seeds at the temperature change condition of 15 ℃/30 ℃ for 10-12 days continuously; and (3) illumination treatment: continuously illuminating the chicory seeds for 24 hours every day at the illumination intensity of 6000lx within the culture period of temperature-changing treatment for 10-12 days, and germinating the seeds. The invention provides a method for promoting the germination of chicory seeds, which is simple to operate and short in germination time, and belongs to a method for improving the germination rate of the chicory seeds. The method has the advantages of high germination rate, short germination time and low cost when the chicory seeds are treated, and can well meet the large-scale production requirement of the chicory.
Description
Technical Field
The invention belongs to the technical field of methods for promoting seed germination, and particularly relates to a method for promoting seed germination of lactuca sativa.
Background
Lactuca (Lactuca tatarica) belongs to Compositae (Compositae), Lactuca L.s.lat., and Mongolian lettuce, sow thistle and Philippine violet lettuce belong to perennial herbs and are 30-70 cm high. In the north China, the northwest, the northeast and Xinjiang provinces, the endive is a common seasonal wild vegetable and is a plant with homology of medicine and food which is very popular with common people. The whole herb of the herba cichorii is used as a medicine and can be used for treating diseases such as acute appendicitis, carbuncle species, erysipelas, conjunctival congestion, blood sucking abdominal pain, red leucorrhea and the like, researchers find that the herba cichorii contains compounds such as natural flavonoids, polyphenols and the like, the substances have good effects on resisting cardiovascular and cerebrovascular diseases, resisting inflammation and easing pain, regulating immunity, reducing blood sugar and blood pressure, treating osteoporosis and the like, and the herba cichorii also has certain effects on clearing free radicals in vivo, inhibiting bacteria, resisting oxidation, resisting radiation and the like. Scientific researchers in China find that the medicinal components contained in the aqueous extract and the ethanol extract of the chicory can effectively inhibit the normal growth of human leukemia cells, human lung cancer cells and human liver cancer cells cultured in vitro and can induce the apoptosis of the human leukemia cells, the human lung cancer cells and the human liver cancer cells.
The endive is a popular season wild vegetable which is favored by people in most areas in the north, has high nutritive value, is natural and pollution-free, has homology of medicine and food, and has good development prospect. However, the current development and utilization of the chicory mainly adopt wild resources, the heavy harvest is managed lightly, and the wild resources are seriously damaged. In addition, the germination conditions of the chicory seeds in the wild are harsh, so that the chicory resources are not concentrated in distribution, a large amount of chicory resources are difficult to collect, and the large-scale production is difficult to meet. According to the method for germinating the chicory seeds in the prior art, the germination rate is low when the chicory seeds are treated, the germination time is long, the seed consumption amount is large in actual production, and the cost is high. Therefore, it is an urgent need to meet the requirement of large-scale production of the chicory at present if a method for rapidly germinating the chicory seeds can be found.
Disclosure of Invention
The invention provides a method for promoting the germination of the chicory seeds, which is simple to operate and short in germination time, in order to overcome the defects in the prior art.
The invention is realized by the following technical scheme: a method for promoting the germination of chicory seeds specifically comprises the following steps:
(1) seed soaking: firstly, soaking the seeds of the chicory in distilled water for 1-5 hours, and then soaking the seeds of the chicory;
(2) culturing: placing the soaked chicory seeds in a culture dish for culture, and placing two layers of filter paper in the culture dish;
(3) temperature change treatment: culturing the chicory seeds at the temperature change condition of 15 ℃/30 ℃ for 10-12 days continuously;
(4) and (3) illumination treatment: and (4) continuously illuminating the chicory seeds for 24 hours every day within the culture period of the temperature-changing treatment in the step (3), wherein the illumination intensity is 6000lx, and the continuous illumination treatment is carried out for 10-12 days, so that the seeds germinate.
Preferably, in the step (1), the chicory seeds are soaked in distilled water for 5 hours to carry out seed soaking treatment on the chicory seeds.
Preferably, in the step (2), the culture dish is a laboratory round culture dish with a diameter of 9 cm.
Preferably, in the step (3), the temperature of the light-temperature automatic control incubator is set to be 15 ℃ and 30 ℃, the chicory seeds are subjected to alternating temperature change treatment, the temperature is 15 ℃ for 12 hours, the temperature is 30 ℃ for 12 hours, and the temperature change treatment is continuously carried out for 10 days.
Preferably, in step (4), the light treatment is continued for 12 days.
Table 1 shows the influence of temperature-variable treatment on the germination indexes of the chicory seeds, and the results in Table 1 show that when the chicory seeds are cultured under the temperature-variable condition of 15 ℃/30 ℃, the germination rate is highest, the germination vigor is best, the germination index is highest, and the starting days are shortest.
Table 1: influence of temperature change on sprouting index of chicory seeds
| Temperature/. degree.C | The germination percentage is% | The germination potential% | Index of germination | Days of origin |
| 15℃ | 4.44c | 0f | 0.74f±0.05 | 9 |
| 15/20℃ | 6.66c | 3.3e±0.05 | 3.23e±0.06 | 5 |
| 15/25℃ | 21.1b | 10.0d±0.07 | 3.78d±0.05 | 5 |
| 10/25℃ | 47.63a | 11.1c±0.04 | 4.72c±0.07 | 6 |
| 15/30℃ | 54.4a | 45.5a±0.06 | 10.39a±0.06 | 3 |
| 20/35℃ | 47.73a | 41.1b±0.08 | 9.44b±0.04 | 4 |
The invention has the beneficial effects that: the invention provides a method for promoting the germination of chicory seeds, which is simple to operate and short in germination time, and belongs to a method for improving the germination rate of the chicory seeds. The technical scheme of the invention overcomes the defect that the development and utilization of the chicory in the prior art can only be wild, so that the chicory which has high nutritive value, is natural and pollution-free and has homology of medicine and food has good development prospect. The technical scheme of the invention also overcomes the defects of harsh field germination conditions of the chicory seeds, non-centralized distribution of the chicory resources, difficult acquisition of a large amount, difficult large-scale production and the like. The method for promoting the germination of the chicory seeds has the advantages of high germination rate, short germination time and low cost when the chicory seeds are treated, is a method for rapidly germinating the chicory seeds, and can well meet the requirement of large-scale production of the chicory.
Detailed Description
The present invention will be described in detail with reference to specific embodiments.
The invention discloses a method for promoting the germination of chicory seeds, which specifically comprises the following steps: (1) seed soaking: firstly, soaking the seeds of the chicory in distilled water for 1-5 hours, and then soaking the seeds of the chicory; (2) culturing: placing the soaked chicory seeds in a culture dish for culture, and placing two layers of filter paper in the culture dish; (3) temperature change treatment: culturing the chicory seeds at the temperature change condition of 15 ℃/30 ℃ for 10-12 days continuously; (4) and (3) illumination treatment: and (4) continuously illuminating the chicory seeds for 24 hours every day within the culture period of the temperature-changing treatment in the step (3), wherein the illumination intensity is 6000lx, and the continuous illumination treatment is carried out for 10-12 days, so that the seeds germinate.
Preferably, the chicory seeds are soaked in distilled water for 5 hours to carry out seed soaking treatment on the chicory seeds. The culture dish is a laboratory round culture dish with the diameter of 9 cm. Setting the temperature of a light-temperature automatic control incubator at 15 ℃ and 30 ℃, carrying out alternate temperature change treatment on the chicory seeds, culturing for 12 hours at 15 ℃, culturing for 12 hours at 30 ℃, and continuously culturing for 10 days by temperature change treatment. The light treatment was continued for 12 days.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (6)
1. A method for promoting the germination of chicory seeds is characterized in that: the method for promoting the germination of the chicory seeds specifically comprises the following steps:
(1) seed soaking:
firstly, soaking the seeds of the chicory in distilled water for 1-5 hours, and then soaking the seeds of the chicory;
(2) culturing:
placing the soaked chicory seeds in a culture dish for culture, and placing two layers of filter paper in the culture dish;
(3) temperature change treatment:
culturing the chicory seeds at the temperature change condition of 15 ℃/30 ℃ for 10-12 days continuously;
(4) and (3) illumination treatment:
and (4) continuously illuminating the chicory seeds for 24 hours every day within the culture period of the temperature-changing treatment in the step (3), wherein the illumination intensity is 6000lx, and the continuous illumination treatment is carried out for 10-12 days, so that the seeds germinate.
2. Method for promoting the germination of chicory seeds according to claim 1, characterized in that: in the step (1), the chicory seeds are soaked in distilled water for 5 hours to carry out seed soaking treatment on the chicory seeds.
3. Method for promoting the germination of chicory seeds according to claim 1, characterized in that: in the step (2), the culture dish is a laboratory round culture dish with the diameter of 9 cm.
4. Method for promoting the germination of chicory seeds according to claim 1, characterized in that: in the step (3), setting the temperature of the light-temperature automatic control incubator to be 15 ℃ and 30 ℃, carrying out alternate temperature change treatment on the chicory seeds, culturing for 12 hours at 15 ℃ and culturing for 12 hours at 30 ℃.
5. Method for promoting the germination of chicory seeds according to claim 1, characterized in that: in the step (3), the temperature-changing treatment is carried out for continuous culture for 10 days.
6. Method for promoting the germination of chicory seeds according to claim 1, characterized in that: in the step (4), the treatment is carried out for 12 days by continuous illumination.
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| CN202010785869.7A CN111869364A (en) | 2020-08-04 | 2020-08-04 | Method for promoting germination of chicory seeds |
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