CN111849757A

CN111849757A - Multifunctional microorganism culture device and culture method

Info

Publication number: CN111849757A
Application number: CN202010798321.6A
Authority: CN
Inventors: 黄国莲; 袁红; 程巧霞
Original assignee: Shaoxing Dalengken Biotechnology Co ltd
Current assignee: Shaoxing Dalengken Biotechnology Co ltd
Priority date: 2020-08-11
Filing date: 2020-08-11
Publication date: 2020-10-30

Abstract

The invention discloses a multifunctional microorganism culture device and a culture method, comprising an incubator, an illumination unit, a humidification unit, an air inlet filtering unit, a temperature control unit and a refrigeration and heating unit; the incubator comprises an incubator body, a transverse partition plate, a cold chamber, a hot chamber, at least three mounting frames and a screen plate, wherein one side of an opening in the front of the incubator body is movably connected with the chamber door, the inner rear side of the incubator body is divided into a front-side incubation chamber and a rear-side equipment chamber through the transverse partition plate, the equipment chamber is divided into a left-side cold chamber and a right-side hot chamber through a middle-longitudinal partition plate II, the bottom surface of the incubation chamber is provided with four upright posts which are distributed in a rectangular shape, the four upright posts are vertically fixed; temperature humidity control is convenient to more peaceful safety when the temperature promotes can guarantee the quick growth of microorganism, adopts lighting apparatus to carry out the illumination completely, and the time is long easy control, and convenient to use has improved the cultivation efficiency of microorganism.

Description

Multifunctional microorganism culture device and culture method

Technical Field

The invention relates to the technical field of microbial culture, in particular to a multifunctional microbial culture device and a culture method.

Background

In the microbial cultivation process, as the microbes can be propagated faster under the conditions of proper illumination, temperature and humidity, a proper environment needs to be created, in the actual cultivation process, the illumination time is not easy to control due to the natural illumination under the condition of need, in the temperature control process, the temperature needs to be controlled by means of refrigeration or heating wire heating, more refrigeration and heating equipment is needed, the temperature of the heating wire is higher, the temperature is not controlled well, and the cultivation efficiency of the microbes is reduced.

Disclosure of Invention

The invention aims to overcome the defects in the prior art, provides a multifunctional microorganism culture device and a culture method, is convenient to control temperature and humidity, is more gentle and safer when the temperature is increased, can ensure the rapid growth of microorganisms, completely adopts lighting equipment for illumination, is easy to control the illumination time, is convenient to use, improves the culture efficiency of the microorganisms, and can effectively solve the problems in the background art.

In order to achieve the purpose, the invention provides the following technical scheme: the multifunctional microorganism culture device comprises an incubator, an illumination unit, a humidification unit, an air inlet filtering unit, a temperature control unit and a refrigeration and heating unit;

the incubator comprises an incubator body, a transverse partition plate, a cold chamber, a hot chamber, a temperature sensor, a humidity sensor, a door, a controller and a controller, wherein one side of an opening in the front of the incubator body is movably connected with the door, the inner rear side of the incubator body is divided into a front-side incubation chamber and a rear-side equipment chamber through the transverse partition plate, the equipment chamber is divided into a left-side cold chamber and a right-side hot chamber through a middle-longitudinal partition plate, four upright columns which are distributed in a rectangular shape are arranged on the bottom surface of the incubation chamber, at least three installation frames are vertically fixed on the four upright columns at equal intervals, a screen plate is arranged on the inner side of each installation frame, a controller is installed on;

cultivate the illumination unit that the both sides in chamber are installed respectively and are corresponded with the otter board, and the top of incubator installs the humidification unit that is used for cultivating the chamber humidification, the air inlet filter unit is installed respectively to two through-holes that the top of incubator corresponds cold cavity and hot cavity, install refrigeration heating unit in the equipment cavity, the side is provided with respectively and cultivates the chamber intercommunication around cold cavity and the hot cavity, with the logical groove of outside intercommunication, with two logical groove bottom swing joint who cultivates the chamber intercommunication have inside baffle, with two logical groove bottom swing joint of outside intercommunication have outside baffle, the switching of controlling inside baffle and outside baffle in the equipment cavity is installed to the temperature control unit.

The temperature sensor and the humidity sensor can transmit the temperature and the humidity in the culture cavity into electric signals to be transmitted to the controller, the controller can control related equipment to adjust the temperature and the humidity, the inoculated culture dish can be placed on the screen plate, the mounting frame can prevent the culture dish from sliding off the screen plate, the illumination unit is controlled by the controller to simulate natural illumination on microorganisms in the culture dish, the illumination duration is conveniently controlled, the humidification unit can add humidity in the culture cavity to facilitate the maintenance of a humidity range suitable for the propagation and growth of the microorganisms, the refrigeration and heating unit adopts the existing air conditioning principle, can adopt the low temperature of the evaporator to control the lower temperature in the culture cavity and can also adopt the high temperature of the condenser to control the higher temperature in the culture cavity, and can select low-temperature air around the evaporator in the cooling cavity to blow into the culture cavity through the temperature control unit, or high-temperature air around the condenser in the heating cavity is blown to the culture cavity, so that the control is convenient and quick.

Further, still include ultraviolet tube and transparent protection tube, the bottom at the otter board is fixed to the transparent protection tube, and the internally mounted of transparent protection tube has ultraviolet tube, ultraviolet tube's input electricity connection controller's output. Before putting into the microorganism and cultivateing, can disinfect other bacteriums of cultivateing the intracavity through the ultraviolet ray of ultraviolet tube, can avoid other bacteriums to influence the growth of required cultivation microorganism, can protect ultraviolet tube through transparent protection tube, avoid being damaged.

Further, the illumination unit includes fixed plate, illumination fluorescent tube, V-arrangement reflector panel and transparent lamp shade, the inner wall at the incubator is fixed to the fixed plate, the side of fixed plate is fixed with the V-arrangement reflector panel, and there is the reflector surface the inboard of V-arrangement reflector panel, and the illumination fluorescent tube is installed to the inboard of V-arrangement reflector panel, and the curved transparent lamp shade is installed to the tip of V-arrangement reflector panel, the output of illumination fluorescent tube's input electricity connection director. Through fixed plate installation V-arrangement reflector panel, the inboard reflection of light mirror surface of V-arrangement reflector panel can go out the light reflection of illumination fluorescent tube, promotes illumination intensity, and transparent lamp shade can protect the illumination fluorescent tube, avoids steam harm illumination fluorescent tube.

Furthermore, the humidifying unit comprises a water tank, a water injection pipe, a steam outlet pipe, an ultrasonic generator, a blower, a dovetail rail groove, a floating block and an ultrasonic oscillator, the water tank is fixed on the incubator, and the top of the water tank is provided with a water injection pipe, the outer side of the water tank is provided with an ultrasonic generator, the input end of the ultrasonic generator is electrically connected with the output end of the controller, the output end of the ultrasonic generator is electrically connected with the input end of the ultrasonic oscillator, the inner side surface of the water tank is provided with a vertical dovetail rail groove, the dovetail rail groove is connected with a dovetail slide bar on the side surface of the floating block in a sliding way, the ultrasonic oscillator is fixed on the floating block, the top of water tank corresponds ultrasonic oscillator and is provided with out the steam pipe, go out the import of steam pipe and air-blower, the air-blower is fixed at the incubator top and export extends to the culture chamber. Produce the ultrasonic wave through supersonic generator, then amplify the water to the water tank in with the ultrasonic wave through ultrasonic oscillator and oscillate to produce water smoke, carry on spacingly through forked tail rail groove to the floating block, the floating block can guarantee that ultrasonic oscillator floats at the surface of water, thereby the water smoke that conveniently produces floats, and the air-blower can carry steam to cultivateing the chamber.

Furthermore, the humidification unit still includes exhaust pipe and steam outlet, the exhaust pipe array sets up at the top of cultivateing the chamber, and the exit linkage of exhaust pipe and air-blower, the bottom of exhaust pipe is equipped with steam outlet. The steam exhaust pipe is used for conveying water mist and uniformly releasing the water mist to the culture cavity through the steam outlet.

Further, the air inlet filter unit includes cartridge filter, filter screen one, dust discharge port, branch one, filter screen two, branch two and inlet fan, the cartridge filter is fixed and is corresponded two through-holes department of cold cavity and hot cavity at the top of incubator, the inboard top of cartridge filter is fixed with the filter screen one of toper structure through branch one, and the dust discharge port has been seted up to all sides of cartridge filter corresponding bottom border department of filter screen one etc. and the inboard middle part of cartridge filter is fixed with discoid filter screen two through branch two, inlet fan is installed to the cartridge filter inboard, inlet fan's input electric connection director's output. The fan of advancing can make the entering into cold chamber and the hot chamber that outside air lasts, and two pairs of filtration of filter screen one and filter screen are to air filter, avoid the dust in the air or other microorganism to enter into the cultivation chamber through equipment by force, can discharge through the dust discharge port when the dust at filter screen one top is more.

Further, the air inlet filter unit still includes down tube and top cap, the top of cartridge filter is connected with the top cap of umbelliform through being no less than three down tube. Can avoid other things directly to fall into the cartridge filter through the top cap in, support through the down tube and can make things convenient for the air to enter into the cartridge filter from the space between top cap and the cartridge filter.

Further, the refrigeration and heating unit includes compressor, condenser, expansion valve, evaporimeter and mount, and the condenser passes through the mount to be fixed at the upside of hot cavity, and the evaporimeter passes through the mount to be fixed at the upside of cold cavity, the exit linkage of evaporimeter import of compressor, the exit linkage of compressor import of condenser, the export of condenser passes through the import that the expansion valve connects the evaporimeter, and the input electric connection controller's of compressor output. The basic principle of the air conditioner is adopted, the lower temperature of the evaporator and the higher temperature of the condenser are used, extra heating wires are not needed for heating, and the high-temperature source and the low-temperature source are convenient to adopt.

Further, the temperature control unit comprises a control motor, a gear, racks, connecting rods, sliding rails, sliding bars, telescopic rods and movable rods, the tops of the internal baffle and the external baffle on the front side and the back side of the cold chamber are respectively movably connected with the two ends of one movable rod, the tops of the internal baffle and the external baffle on the front side and the back side of the hot chamber are respectively movably connected with the two ends of the other movable rod, the middle parts of the two movable rods are respectively movably connected with the top ends of the two telescopic rods, the bottom ends of the two telescopic rods are fixedly connected with the two sliding bars, the two sliding bars are respectively slidably connected with the sliding rails fixed in the hot chamber and the cold chamber, one sides of the two sliding bars close to the second partition plate are respectively connected with the two racks through the connecting rods, the control motor is installed in the groove on the second partition plate, the output shaft of the control motor is, the input end of the control motor is electrically connected with the output end of the controller. If the microorganism needs higher temperature, the controller controls the control motor to rotate in the forward direction to drive the gear to rotate, the two racks move in the reverse direction, the internal baffle of the cold chamber can block the through groove through the transmission of the connecting rod and the telescopic rod, the external baffle of the cold chamber can open the through groove, the internal baffle of the hot chamber can open the through groove, the external baffle of the hot chamber can block the through groove, and the heat emitted by the condenser in the hot chamber is blown into the culture chamber through the through groove by the air inlet fan in the air inlet filtering unit; if the microorganism needs lower temperature, the controller controls the control motor to rotate in a rotating direction, finally the air inlet fan brings cold air around the evaporator in the cold chamber into the culture chamber, and the temperature sensor can monitor the temperature in the culture chamber, so that the operation of a compressor in the refrigerating and heating unit is controlled by the controller;

the culture method of the multifunctional microorganism culture device comprises the following steps;

s1: pre-sterilization, namely opening an ultraviolet lamp tube through a controller to sterilize and disinfect the culture cavity so as to ensure that the environment of the culture cavity is clean;

s2: placing the inoculated culture dish on the screen plate, closing the box door, and controlling the illumination time of the illumination lamp tube in the illumination unit through the controller according to the requirement;

s3: if the microorganism needs higher temperature, the controller controls the control motor to rotate in the forward direction to drive the gear to rotate, the two racks move in the reverse direction, the internal baffle of the cold chamber can block the through groove through the transmission of the connecting rod and the telescopic rod, the external baffle of the cold chamber can open the through groove, the internal baffle of the hot chamber can open the through groove, the external baffle of the hot chamber can block the through groove, and the heat emitted by the condenser in the hot chamber is blown into the culture chamber through the through groove by the air inlet fan in the air inlet filtering unit;

s4: if the microorganism needs lower temperature, the controller controls the control motor to rotate in a rotating direction, finally the air inlet fan brings cold air around the evaporator in the cold chamber into the culture chamber, and the temperature sensor can monitor the temperature in the culture chamber, so that the operation of a compressor in the refrigerating and heating unit is controlled by the controller;

s5: the humidity is controlled, the humidity in the culture cavity is detected through the humidity sensor, when the humidity is insufficient, the ultrasonic generator and the blower are controlled to operate through the controller, the ultrasonic oscillator amplifies and oscillates ultrasonic waves of the ultrasonic generator so as to vaporize water in the water tank, and then water vapor is conveyed to the culture cavity through the blower.

Compared with the prior art, the invention has the beneficial effects that: the multifunctional microorganism culture device and the culture method have the following advantages:

1. the temperature sensor and the humidity sensor can transmit the temperature and the humidity in the culture cavity into electric signals to be transmitted to the controller, the controller can control related equipment to adjust the temperature and the humidity, the inoculated culture dish can be placed on the screen plate, and the mounting frame can prevent the culture dish from sliding off the screen plate;

2. the illumination unit is controlled by the controller, natural illumination is simulated for microorganisms in the culture dish, illumination duration is conveniently controlled, the humidification unit can add humidity into the culture cavity, a humidity range suitable for propagation and growth of the microorganisms is kept, the refrigeration and heating unit adopts the existing air conditioning principle, the low temperature of the evaporator can be adopted to control the low temperature in the culture cavity, the high temperature of the condenser can be adopted to control the high temperature in the culture cavity, low-temperature air around the evaporator in the cooling cavity can be selectively blown into the culture cavity through the temperature control unit, or high-temperature air around the condenser in the heating cavity can be blown into the culture cavity through the temperature control unit, and control is convenient and rapid;

3. temperature humidity control is convenient to more peaceful safety when the temperature promotes can guarantee the quick growth of microorganism, adopts lighting apparatus to carry out the illumination completely, and the time is long easy control, and convenient to use has improved the cultivation efficiency of microorganism.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic bottom view of the present invention;

FIG. 3 is a schematic rear side view of the present invention;

FIG. 4 is a schematic rear sectional view of the present invention;

FIG. 5 is an enlarged view of a portion of FIG. 4;

FIG. 6 is a schematic diagram of a second embodiment of the present invention;

FIG. 7 is an enlarged cross-sectional view of the intake air filter unit of FIG. 6 according to the present invention;

FIG. 8 is a schematic cross-sectional view of a water tank according to the present invention.

In the figure: 1 incubator, 2 chamber door, 3 upright post, 4 illumination unit, 41 fixed plate, 42 lighting lamp tube, 43V-shaped reflector, 44 transparent lampshade, 5 humidification unit, 51 water tank, 52 water injection tube, 53 steam outlet tube, 54 ultrasonic generator, 55 blower, 56 steam exhaust tube, 57 steam outlet, 58 dovetail rail groove, 59 floating block, 510 ultrasonic oscillator, 6 air inlet filter unit, 61 filter cartridge, 62 diagonal rod, 63 top cover, 64 filter screen I, 65 dust outlet, 66 support rod I, 67 filter screen II, 68 support rod II, 69 air inlet fan, 7 temperature control unit, 71 control motor, 72 gear, 73 rack, 74 connecting rod, 75 slide rail, 76 slide bar, 77 telescopic rod, 78 movable rod, 8 refrigeration and heating unit, 81 compressor, 82 condenser, 83 expansion valve, 84 evaporator, 85 fixed frame, 9 mounting frame, 10 screen plate, 11 ultraviolet lamp tube, 12 transparent protection tube, 13 temperature sensor, 14 humidity transducer, 15 controller, 16 partition board one, 17 partition board two, 18 external baffle, 19 internal baffle, 20 through groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: the multifunctional microorganism culture device comprises an incubator 1, an illumination unit 4, a humidification unit 5, an air inlet filtering unit 6, a temperature control unit 7 and a refrigeration and heating unit 8;

one side of an opening in the front of the incubator 1 is movably connected with a chamber door 2, the rear side in the incubator 1 is divided into a front-side incubation cavity and a rear-side equipment cavity by a transverse first division plate 16, the equipment cavity is divided into a left-side cold cavity and a right-side hot cavity by a middle longitudinal second division plate 17, four upright posts 3 which are distributed in a rectangular shape are arranged on the bottom surface of the incubation cavity, at least three mounting frames 9 are vertically fixed on the four upright posts 3 at equal intervals, a screen plate 10 is arranged on the inner side of each mounting frame 9, a controller 15 is arranged on the outer side surface of the incubator 1, a temperature sensor 13 and a humidity sensor 14 are respectively arranged on each upright post 3, and output ends of the temperature sensor 13 and the humidity sensor 14 are;

cultivate the illumination unit 4 that the both sides in chamber are installed respectively and are corresponded with otter board 10, and incubator 1's top is installed and is used for the humidification unit 5 to cultivateing the chamber humidification, incubator 1's top corresponds two through-holes of cold cavity and hot cavity and installs air inlet filter unit 6 respectively, install refrigeration heating unit 8 in the equipment cavity, the front and back side of cold cavity and hot cavity is provided with respectively and cultivates the chamber intercommunication, logical groove 20 with outside intercommunication, it has inside board 19 to lead to groove 20 bottom swing joint with two of cultivateing the chamber intercommunication, it has outside baffle 18 to lead to groove 20 bottom swing joint with two of outside intercommunication, the switching of inside board 19 and outside baffle 18 is controlled at equipment intracavity to temperature control unit 7.

The temperature sensor 13 and the humidity sensor 14 can convert the temperature and humidity in the culture cavity into electric signals to be transmitted to the controller 15, the controller 15 can control related equipment to adjust the temperature and humidity, the inoculated culture dish can be placed on the screen plate 10, the mounting frame 9 can prevent the culture dish from sliding off the screen plate 10, the illumination unit 4 is controlled by the controller 15 to simulate natural illumination on microorganisms in the culture dish, so that the illumination duration is conveniently controlled, the humidification unit 5 can add humidity in the culture cavity to facilitate maintaining a humidity range suitable for propagation and growth of the microorganisms, the refrigeration and heating unit 8 adopts the existing air-conditioning principle, can adopt the low temperature of an evaporator to control the lower temperature in the culture cavity, can also adopt the high temperature of a condenser to control the higher temperature in the culture cavity, and can select low-temperature air around the evaporator in the cooling cavity to be blown into the culture cavity through the temperature control unit 7, or high-temperature air around the condenser in the heating cavity is blown to the culture cavity, so that the control is convenient and quick.

Referring to fig. 1, the display device further includes an ultraviolet lamp tube 11 and a transparent protection tube 12, the transparent protection tube 12 is fixed at the bottom of the screen 10, the ultraviolet lamp tube 11 is installed inside the transparent protection tube 12, and an input end of the ultraviolet lamp tube 11 is electrically connected to an output end of the controller 15. Before putting into microorganism and cultivateing, can disinfect to other bacteriums of cultivateing the intracavity through the ultraviolet ray of ultraviolet tube 11, can avoid other bacteriums to influence the growth of required cultivation microorganism, can protect ultraviolet tube 11 through transparent protection tube 12, avoid being damaged.

The illumination unit 4 comprises a fixing plate 41, an illumination lamp tube 42, a V-shaped reflector 43 and a transparent lampshade 44, the fixing plate 41 is fixed on the inner wall of the incubator 1, the V-shaped reflector 43 is fixed on the side surface of the fixing plate 41, a reflector surface is arranged on the inner side of the V-shaped reflector 43, the illumination lamp tube 42 is arranged on the inner side of the V-shaped reflector 43, the arc-shaped transparent lampshade 44 is arranged at the end part of the V-shaped reflector 43, and the input end of the illumination lamp tube 42 is electrically connected with the output end of the controller 15. Through the V-arrangement reflector panel 43 of fixed plate 41 installation, the inboard reflector surface of V-arrangement reflector panel 43 can go out the light reflection of illumination fluorescent tube 42, promotes illumination intensity, and transparent lamp shade 44 can protect illumination fluorescent tube 42, avoids steam harm illumination fluorescent tube 42.

Referring to fig. 1, 2 and 8, the humidifying unit 5 includes a water tank 51, a water injection pipe 52, a steam outlet pipe 53, an ultrasonic generator 54, a blower 55, a dovetail rail groove 58, a floating block 59 and an ultrasonic oscillator 510, the water tank 51 is fixed on the incubator 1, and the top of the water tank 51 is provided with a water injection pipe 52, the outer side of the water tank 51 is provided with an ultrasonic generator 54, the input end of the ultrasonic generator 54 is electrically connected with the output end of the controller 15, and the output of supersonic generator 54 is electrically connected with the input of supersonic oscillator 510, the inside side of water tank 51 is installed with vertical forked tail rail groove 58, forked tail rail groove 58 and the forked tail draw runner sliding connection of the floating block 59 side, supersonic oscillator 510 fixes on floating block 59, the top of water tank 51 is provided with steam outlet pipe 53 corresponding to supersonic oscillator 510, steam outlet pipe 53 and the import of air-blower 55, air-blower 55 is fixed at the top of incubator 1 and the export extends to the culture chamber. Produce the ultrasonic wave through supersonic generator 54, then amplify the water to the water tank 51 in with the ultrasonic wave through ultrasonic oscillator 510 and vibrate to produce water smoke, carry on spacingly to floating block 59 through forked tail rail groove 58, floating block 59 can guarantee that ultrasonic oscillator 510 floats at the surface of water, thereby the water smoke that conveniently produces floats, and air-blower 55 can carry steam to cultivateing the chamber.

The humidifying unit 5 further comprises a steam exhaust pipe 56 and a steam outlet 57, the steam exhaust pipe 56 is arranged at the top of the culture chamber in an array mode, the steam exhaust pipe 56 is connected with an outlet of the blower 55, and the steam outlet 57 is arranged at the bottom of the steam exhaust pipe 56. The steam discharge pipe 56 is used for delivering water mist and uniformly discharging the water mist to the culture chamber through a steam outlet 57.

Referring to fig. 6 and 7, the intake air filtering unit 6 includes a filter cartridge 61, a first filter screen 64, a dust outlet 65, a first support rod 66, a second filter screen 67, a second support rod 68 and an intake fan 69, the filter cartridge 61 is fixed at two through holes of the top of the incubator 1 corresponding to the cold chamber and the hot chamber, the first filter screen 64 with a cone structure is fixed at the top of the inner side of the filter cartridge 61 through the first support rod 66, the dust outlet 65 is arranged at the peripheral side of the filter cartridge 61 corresponding to the bottom edge of the first filter screen 64 at an equal angle, the second disc-shaped filter screen 67 is fixed at the middle of the inner side of the filter cartridge 61 through the second support rod 68, the intake fan 69 is installed at the inner side of. The air inlet fan 69 can make the outside air continuously enter the cold chamber and the hot chamber, the first filter screen 64 and the second filter screen 67 filter the air, so that dust or other microorganisms in the air are prevented from entering the culture chamber through the device, and when more dust exists on the top of the first filter screen 64, the dust can be discharged through the dust outlet 65.

The intake air filter unit 6 further comprises an inclined rod 62 and a top cover 63, and the top of the filter cartridge 61 is connected with the umbrella-shaped top cover 63 through not less than three inclined rods 62. Other things can be prevented from directly falling into the filter cartridge 61 through the top cover 63, and the air can be conveniently introduced into the filter cartridge 61 from the gap between the top cover 63 and the filter cartridge 61 through the support of the inclined rod 62.

Referring to fig. 4, the cooling and heating unit 8 includes a compressor 81, a condenser 82, an expansion valve 83, an evaporator 84 and a fixing frame 85, the condenser 82 is fixed on the upper side of the hot chamber through the fixing frame 85, the evaporator 84 is fixed on the upper side of the cold chamber through the fixing frame 85, an outlet of the evaporator 84 is connected to an inlet of the compressor 81, an outlet of the compressor 81 is connected to an inlet of the condenser 82, an outlet of the condenser 82 is connected to an inlet of the evaporator 84 through the expansion valve 83, and an input end of the compressor 81 is electrically connected to an output end of the controller 15. By adopting the basic principle of air conditioning, the lower temperature of the evaporator 84 and the higher temperature of the condenser 82 are used, no extra heating wires are needed for heating, and the high-low temperature source is convenient to use.

Referring to fig. 5, the temperature control unit 7 includes a control motor 71, a gear 72, a rack 73, a connecting rod 74, a sliding rail 75, a sliding bar 76, an expansion rod 77 and a movable rod 78, the tops of the internal baffle 19 and the external baffle 18 at the front and back sides of the cold chamber are respectively movably connected with two ends of one movable rod 78, the tops of the internal baffle 19 and the external baffle 18 at the front and back sides of the hot chamber are respectively movably connected with two ends of another movable rod 78, the middle parts of the two movable rods 78 are respectively movably connected with the top ends of the two expansion rods 77, the bottom ends of the two expansion rods 77 are fixedly connected with the two sliding bars 76, the two sliding bars 76 are respectively slidably connected with the sliding rail 75 fixed in the hot chamber and the cold chamber, one side of the two sliding bars 76 near the partition plate two 17 is respectively connected with two racks 73 through the connecting rod 74, the control motor 71 is installed in the slot on the, two sides of the gear 72 are respectively engaged with the two racks 73, and the input end of the control motor 71 is electrically connected with the output end of the controller 15. If the microorganism needs higher temperature, the controller 15 controls the control motor 71 to rotate in the forward direction to drive the gear 72 to rotate, the two racks 73 move in the reverse direction, the internal baffle plate 19 of the cold chamber can block the through groove 20 through the transmission of the connecting rod 74 and the telescopic rod 77, the external baffle plate 18 of the cold chamber can open the through groove 20, the internal baffle plate 19 of the hot chamber can open the through groove 20, the external baffle plate 18 of the hot chamber can block the through groove 20, and the heat emitted by the condenser 82 in the hot chamber is blown into the culture chamber through the through groove 20 by the air inlet fan 69 in the air inlet filtering unit 6; if the microorganism needs a lower temperature, the controller 15 controls the control motor 71 to rotate, finally the air inlet fan 69 brings the cold air around the evaporator 84 in the cold chamber into the culture chamber, the temperature sensor 13 can monitor the temperature in the culture chamber, and the controller 15 controls the operation of the compressor 81 in the cooling and heating unit 8;

referring to FIGS. 1 to 8, a method for culturing a multi-functional microorganism culture apparatus includes the following steps;

s1: pre-sterilization, namely opening the ultraviolet lamp tube 11 through the controller 15 to sterilize and disinfect the culture cavity, so as to ensure the clean environment of the culture cavity;

s2: placing the inoculated culture dish on the screen plate 10, closing the box door 2, and controlling the illumination time of the illumination lamp tube 42 in the illumination unit 4 through the controller 15 according to the requirement;

s3: if the microorganism needs higher temperature, the controller 15 controls the control motor 71 to rotate in the forward direction to drive the gear 72 to rotate, the two racks 73 move in the reverse direction, the internal baffle plate 19 of the cold chamber can block the through groove 20 through the transmission of the connecting rod 74 and the telescopic rod 77, the external baffle plate 18 of the cold chamber can open the through groove 20, the internal baffle plate 19 of the hot chamber can open the through groove 20, the external baffle plate 18 of the hot chamber can block the through groove 20, and the heat emitted by the condenser 82 in the hot chamber is blown into the culture chamber through the through groove 20 by the air inlet fan 69 in the air inlet filtering unit 6;

s4: if the microorganism needs a lower temperature, the controller 15 controls the control motor 71 to rotate, finally the air inlet fan 69 brings the cold air around the evaporator 84 in the cold chamber into the culture chamber, the temperature sensor 13 can monitor the temperature in the culture chamber, and the controller 15 controls the operation of the compressor 81 in the cooling and heating unit 8;

s5: humidity is controlled, humidity in the culture chamber is detected by the humidity sensor 14, and when the humidity is insufficient, the controller 15 controls the operation of the ultrasonic generator 54 and the blower 55, the ultrasonic oscillator 510 amplifies and oscillates the ultrasonic wave of the ultrasonic generator 54 to vaporize water in the water tank 51, and then the water vapor is sent to the culture chamber by the blower 55.

It should be noted that the electric components of the temperature sensor 13, the humidity sensor 14, the ultraviolet lamp 11, the lighting lamp 42, the ultrasonic generator 54, the ultrasonic oscillator 510, the blower 55, the air intake fan 69, the compressor 81, and the control motor 71 disclosed in this embodiment are all of commercially available types, power can be selected according to actual conditions, the controller 15 is a PLC controller, and the controller 15 controls the temperature sensor 13, the humidity sensor 14, the ultraviolet lamp 11, the lighting lamp 42, the ultrasonic generator 54, the blower 55, the air intake fan 69, the compressor 81, and the control motor 71 to operate by methods commonly used in the prior art.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Multi-functional microbial cultivation device, its characterized in that: comprises an incubator (1), an illumination unit (4), a humidification unit (5), an air inlet filtering unit (6), a temperature control unit (7) and a refrigeration and heating unit (8);

one side of the front opening of the incubator (1) is movably connected with a chamber door (2), the inner rear side of the incubator (1) is divided into an incubation chamber at the front side and a rear equipment chamber by a transverse division plate I (16), and the equipment cavity is divided into a cold cavity at the left side and a hot cavity at the right side by a second partition plate (17) with the longitudinal middle part, four upright posts (3) which are distributed in a rectangular shape are arranged on the bottom surface of the culture cavity, at least three mounting frames (9) are fixed on the four upright posts (3) in a vertical equidistant manner, a screen plate (10) is arranged on the inner side of the mounting frame (9), a controller (15) is arranged on the outer side surface of the incubator (1), a temperature sensor (13) and a humidity sensor (14) are respectively arranged on the upright post (3), the output ends of the temperature sensor (13) and the humidity sensor (14) are electrically connected with the input end of the controller (15);

cultivate the illumination unit (4) that the both sides in chamber are installed respectively and are corresponded with otter board (10), and the top of incubator (1) installs and be used for humidification unit (5) to cultivateing the chamber humidification, air inlet filter unit (6) are installed respectively to two through-holes that the top of incubator (1) corresponds cold chamber and hot chamber, refrigeration heating unit (8) are installed to the equipment intracavity, the side is provided with respectively around cold chamber and the hot chamber with cultivate chamber intercommunication, with logical groove (20) of outside intercommunication, with two logical groove (20) bottom swing joint of cultivateing the chamber intercommunication have inside board (19), with two logical groove (20) bottom swing joint of outside intercommunication have outside baffle (18), temperature control unit (7) are installed and are controlled the switching of inside board (19) and outside baffle (18) in the equipment intracavity.

2. The multifunctional microbial cultivation device according to claim 1, characterized in that: still include ultraviolet tube (11) and transparent protection tube (12), the bottom at otter board (10) is fixed in transparent protection tube (12), and the internally mounted of transparent protection tube (12) has ultraviolet tube (11), the output of the input electric connection controller (15) of ultraviolet tube (11).

3. The multifunctional microbial cultivation device according to claim 1, characterized in that: illumination unit (4) include fixed plate (41), illumination fluorescent tube (42), V-arrangement reflector panel (43) and transparent lamp shade (44), the inner wall at incubator (1) is fixed in fixed plate (41), the side of fixed plate (41) is fixed with V-arrangement reflector panel (43), and there is the reflector surface inboard of V-arrangement reflector panel (43), and illumination fluorescent tube (42) are installed to the inboard of V-arrangement reflector panel (43), and curved transparent lamp shade (44) are installed to the tip of V-arrangement reflector panel (43), the output of input electric connection controller (15) of illumination fluorescent tube (42).

4. The multifunctional microbial cultivation device according to claim 1, characterized in that: humidification unit (5) includes water tank (51), water injection pipe (52), play steam pipe (53), supersonic generator (54), air-blower (55), forked tail rail groove (58), floating block (59) and ultrasonic oscillator (510), water tank (51) are fixed on incubator (1), and the top of water tank (51) is provided with water injection pipe (52), supersonic generator (54) is installed to the outside of water tank (51), the output of input electric connection controller (15) of supersonic generator (54), and the input of ultrasonic oscillator (510) is connected to the output electricity of supersonic generator (54), vertical forked tail rail groove (58) is installed to the inside side of water tank (51), forked tail rail groove (58) and the forked tail draw runner sliding connection of floating block (59) side, ultrasonic oscillator (510) are fixed on floating block (59), the top of water tank (51) corresponds ultrasonic oscillator (510) and is provided with steam outlet pipe (53), steam outlet pipe (53) and the import of air-blower (55), air-blower (55) are fixed at incubator (1) top and export and are extended to the cultivation intracavity.

5. The multifunctional microbial cultivation device according to claim 1, characterized in that: humidification unit (5) still include exhaust pipe (56) and steam outlet (57), exhaust pipe (56) array sets up at the top of cultivateing the chamber, and exhaust pipe (56) and blower (55) exit linkage, the bottom of exhaust pipe (56) is equipped with steam outlet (57).

6. The multifunctional microbial cultivation device according to claim 1, characterized in that: the air inlet filtering unit (6) comprises a filtering cylinder (61), a first filtering net (64), a dust outlet (65), a first supporting rod (66), a second filtering net (67), a second supporting rod (68) and an air inlet fan (69), the filter cartridge (61) is fixed at the top of the incubator (1) corresponding to two through holes of the cold chamber and the hot chamber, a first filter screen (64) with a conical structure is fixed at the top of the inner side of the filter cartridge (61) through a first support rod (66), dust discharge ports (65) are arranged at the positions of the peripheral side of the filter cartridge (61) corresponding to the bottom edge of the first filter screen (64) at equal angles, a second disk-shaped filter screen (67) is fixed at the middle part of the inner side of the filter cylinder (61) through a second support rod (68), an air inlet fan (69) is installed on the inner side of the filter cylinder (61), and the input end of the air inlet fan (69) is electrically connected with the output end of the controller (15).

7. The multifunctional microbial cultivation device according to claim 1, characterized in that: the air inlet filter unit (6) still includes down tube (62) and top cap (63), the top of cartridge filter (61) is connected with umbelliform top cap (63) through being no less than three down tube (62).

8. The multifunctional microbial cultivation device according to claim 1, characterized in that: refrigeration heating unit (8) include compressor (81), condenser (82), expansion valve (83), evaporimeter (84) and mount (85), and upside at hot cavity is fixed through mount (85) in condenser (82), and evaporimeter (84) are fixed on the upside of cold cavity through mount (85), the import of exit linkage compressor (81) of evaporimeter (84), the import of exit linkage condenser (82) of compressor (81), the import of evaporimeter (84) is connected through expansion valve (83) in the export of condenser (82), the output of the input electric connection controller (15) of compressor (81).

9. The multifunctional microbial cultivation device according to claim 1, characterized in that: the temperature control unit (7) comprises a control motor (71), a gear (72), racks (73), connecting rods (74), sliding rails (75), sliding bars (76), telescopic rods (77) and movable rods (78), the tops of an internal baffle (19) and an external baffle (18) on the front side and the back side of the cold chamber are respectively movably connected with two ends of one movable rod (78), the tops of the internal baffle (19) and the external baffle (18) on the front side and the back side of the hot chamber are respectively movably connected with two ends of the other movable rod (78), the middle parts of the two movable rods (78) are respectively movably connected with the top ends of the two telescopic rods (77), the bottom ends of the two telescopic rods (77) are fixedly connected with the two sliding bars (76), the two sliding bars (76) are respectively slidably connected with the sliding rails (75) fixed in the hot chamber and the cold chamber, one sides of the two sliding bars (76) close to the partition plates (17) are respectively connected with the two racks (73) through the connecting rods, a control motor (71) is installed in a notch on the second partition plate (17), an output shaft of the control motor (71) is connected with a gear (72), two sides of the gear (72) are respectively meshed with the two racks (73), and an input end of the control motor (71) is electrically connected with an output end of the controller (15).

10. The method for culturing the multifunctional microorganism culture apparatus according to any one of claims 1 to 9, wherein: comprises the following steps;

s1: pre-sterilization and disinfection, wherein the ultraviolet lamp tube (11) is opened through the controller (15) to sterilize and disinfect the culture cavity, so that the environment of the culture cavity is ensured to be clean;

s2: placing the inoculated culture dish on the screen plate (10), and controlling the illumination time of an illumination lamp tube (42) in the illumination unit (4) through a controller (15) according to the requirement after closing the box door (2);

s3: if the microorganism needs higher temperature, the controller (15) controls the control motor (71) to rotate forward to drive the gear (72) to rotate, the two racks (73) move reversely, the internal baffle (19) of the cold chamber can block the through groove (20) through the transmission of the connecting rod (74) and the telescopic rod (77), the external baffle (18) of the cold chamber can open the through groove (20) and the internal baffle (19) of the hot chamber can open the through groove (20) and the external baffle (18) of the hot chamber can block the through groove (20), and heat emitted by the condenser (82) in the hot chamber is blown into the culture chamber through the through groove (20) by the air inlet fan (69) in the air inlet filtering unit (6);

s4: if the microorganism needs lower temperature, the controller (15) controls the control motor (71) to rotate in a direction, finally the air inlet fan (69) brings cold air around the evaporator (84) in the cold chamber into the culture chamber, the temperature sensor (13) can monitor the temperature in the culture chamber, and therefore the controller (15) controls the operation of the compressor (81) in the refrigerating and heating unit (8);

s5: humidity is controlled, humidity in the culture cavity is detected through a humidity sensor (14), when the humidity is insufficient, an ultrasonic generator (54) and a blower (55) are controlled to operate through a controller (15), the ultrasonic oscillator (510) amplifies and oscillates ultrasonic waves of the ultrasonic generator (54) so as to vaporize water in a water tank (51), and then water vapor is conveyed to the culture cavity through the blower (55).