CN111849756B

CN111849756B - Multifunctional aseptic constant-temperature incubator for microbial separation

Info

Publication number: CN111849756B
Application number: CN202010793146.1A
Authority: CN
Inventors: 胡莉; 周明; 王明成; 鲁珍; 李恩中
Original assignee: Huanghuai University
Current assignee: Huanghuai University
Priority date: 2020-08-07
Filing date: 2020-08-07
Publication date: 2022-09-27
Anticipated expiration: 2040-08-07
Also published as: CN111849756A

Abstract

The invention relates to a multifunctional aseptic constant-temperature incubator for separating microorganisms, which comprises a box body with a base, wherein the box body is provided with a bottom cavity for mounting electrical elements, a storage cavity for storing culture dishes and a culture cavity for culturing microorganisms; the storage cavity is divided into at least two placing units by a partition plate, and a first ultraviolet disinfection lamp is arranged on the side wall of each placing unit; the culture chamber is longitudinally provided with a plurality of culture dish placing assemblies capable of moving back and forth, the rear side wall of the culture chamber is symmetrically provided with two housings, a certain distance is reserved between the two housings to form a lifting space, and a camera assembly capable of lifting is arranged in the lifting space; the invention has the advantages of convenient use, multiple functions, strong flexibility and convenient temperature adjustment.

Description

Multifunctional aseptic constant-temperature incubator for microbial separation

Technical Field

The invention belongs to the technical field of equipment for microbial separation, and particularly relates to a multifunctional sterile constant-temperature incubator for microbial separation.

Background

The microorganism separating and purifying mold is a basic method for researching microorganisms; specific microbial individuals are isolated from a population or from a heterogeneous population of microorganisms, and only one population of microorganisms from the same ancestor is allowed to survive in a specific environment. The microorganism isolation method generally comprises the following five methods: pour plate method, spread plate method, plate-scribing method, enrichment culture method, anaerobic method. The pouring plate method includes the steps of firstly diluting a microorganism suspension in series, taking a certain amount of diluent to be fully mixed with a melted nutrient agar culture medium which is kept at about 40-50 degrees, then pouring the mixed liquid into a sterile culture dish, after the mixed liquid is solidified, inverting the plate in a thermostat for culture, forming bacterial colonies after multiple proliferation of single cells, taking a single bacterial colony supporting suspension, and repeating the steps for multiple times to obtain a pure culture.

In the prior art, the constant temperature incubators used in the pouring plate method for separating microorganisms are various in types, but the constant temperature incubators are single in function, so that the working conditions in the incubators cannot be visually observed conveniently, selective operation on part of culture dishes in the incubators cannot be performed conveniently, and great convenience is brought to the separation culture operation of bacteria; moreover, the current constant temperature incubator cannot share data in real time, and cannot realize remote operation of the constant temperature incubator.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provide the multifunctional aseptic constant-temperature incubator for separating the microorganisms, which is convenient to use, has multiple functions and strong flexibility and is convenient for temperature adjustment.

The technical scheme of the invention is as follows:

a multifunctional aseptic constant-temperature incubator for separating microorganisms comprises a box body with a base, wherein the box body is provided with a bottom cavity for mounting electrical elements, a storage cavity for storing culture dishes and a culture cavity for culturing microorganisms;

the storage cavity is divided into at least two placing units by a partition plate, and a first ultraviolet disinfection lamp is arranged on the side wall of each placing unit;

the culture chamber is longitudinally provided with a plurality of culture dish placing assemblies capable of moving back and forth, two housings are symmetrically arranged on the rear side wall of the culture chamber, a certain distance is reserved between the two housings to form a lifting space, and a camera assembly capable of lifting is arranged in the lifting space;

the culture dish placing assembly comprises slide rails symmetrically arranged on the left side wall and the right side wall of the culture cavity and a placing plate capable of moving back and forth along the slide rails;

the sliding rail comprises an upper sliding plate and a lower sliding plate, the upper sliding plate extends along the direction of the rear side of the side wall of the culture cavity, the lower sliding plate is positioned on the lower side of the upper sliding plate, a first boss extending downwards is arranged at one end, away from the upper sliding plate, connected with the side wall of the culture cavity, of the upper sliding plate, a first stop plate extending downwards is arranged at one end, facing the opening of the culture cavity, of the upper sliding plate, a second boss extending upwards is arranged at one end, away from the lower sliding plate, connected with the side wall of the culture cavity, of the lower sliding plate, the first boss and the second boss are positioned on the same vertical plane, a gap for the placing plate to pass through is formed between the first boss and the second boss, and a strip-shaped hole is formed at one end, facing the opening of the culture cavity, of the lower sliding plate positioned on the left side wall of the culture cavity;

an accommodating space is formed between the upper sliding plate and the lower sliding plate;

the placing plate comprises a transverse plate and sliding blocks symmetrically arranged at the left end and the right end of the transverse plate, the sliding blocks are embedded in the accommodating space between the upper side plate and the lower side plate, the transverse plate penetrates through the gap, and teeth are arranged on the lower side surface of the sliding block positioned at the left end of the transverse plate;

a driving gear is arranged on the lower side of the lower sliding plate on the left side wall of the culture cavity, one end of the driving gear extends into the strip-shaped hole, and the driving gear is meshed with teeth on the lower side surface of the sliding block at the left end of the transverse plate;

the driving gear is rotatably connected to the longitudinal partition plate through a first rotating shaft, the longitudinal partition plate is provided with a hollow cavity, and one end, far away from the driving gear, of the first rotating shaft extends into the cavity;

a hollow fixed shaft is arranged in the cavity corresponding to the first rotating shaft, the left end of the fixed shaft has a certain distance with the first rotating shaft, a first chain wheel is arranged at one end of the fixed shaft, which faces the first rotating shaft, in a rotating manner, a first annular stop table extending towards the inside is arranged at the right end of the fixed shaft, a first electromagnet fixed on the inner side wall of the longitudinal clapboard is arranged in the fixed shaft, a push shaft is arranged on the right side of the first electromagnet, the left end of the push shaft is provided with a first magnet, the circumferential surface of the right end of the push shaft is provided with a first bump, a first spring is sleeved on the periphery of the push shaft, two ends of the first spring respectively abut against the first magnet and the first annular stop table, a first notch matched with the first bump is formed in the position, corresponding to the first annular stop table, of the right end of the fixed shaft;

the first chain wheel is provided with a stepped through hole, the right end of the fixed shaft is connected into a large-diameter hole of the stepped through hole through a bearing, the aperture of a small-diameter hole of the stepped through hole is the same as the outer diameter of the push shaft, a second notch corresponding to the first notch is formed in the hole wall of the small-diameter hole of the stepped through hole, the left end of the first rotating shaft is provided with an insertion hole for the push shaft to insert, and a third notch for the first boss to insert is formed in the hole wall of the insertion hole;

a first servo motor is fixed on the upper side wall of the bottom cavity, the output shaft end of the first servo motor is connected with a second chain wheel, and the second chain wheel is connected with the first chain wheel through a chain;

the camera shooting assembly comprises a first multi-wedge belt wheel arranged between two housing shells through a shaft, a second multi-wedge belt wheel positioned in the bottom cavity and corresponding to the first multi-wedge belt wheel, and a second servo motor, wherein the first multi-wedge belt wheel is connected with the second multi-wedge belt wheel through a first multi-wedge belt, a first belt wheel is arranged on the shaft of the second multi-wedge belt wheel, a second belt wheel is arranged on an output shaft of the second servo motor, the first belt wheel is connected with the second belt wheel through a belt, and a camera and a light supplement lamp are arranged on the side surface of the first multi-wedge belt facing the culture cavity;

a window is formed in the right door plate, sealing glass is installed at one end, facing the culture cavity, of the window, and a shielding plate capable of moving up and down is installed at one end, facing away from the culture cavity, of the sealing glass;

the back side wall of the culture cavity corresponding to the housing is provided with electric heating rods and semiconductor refrigerating pieces in a staggered manner from top to bottom;

the left side wall and the right side wall of the culture cavity are respectively provided with a second ultraviolet disinfection lamp and a temperature sensor;

two door second magnets are arranged at one end, facing the opening of the box body, of the longitudinal partition plate, second electromagnets corresponding to the second magnets are arranged on the left door plate and the right door plate, and the second electromagnets are connected with a touch entrance guard switch;

install control circuit board in the end intracavity, control circuit board on install MCU chip and with the wireless network module that the MCU chip is connected, the signal input part of MCU chip with temperature sensor links to each other, the signal output part of MCU chip connects first ultraviolet disinfection lamp, first electromagnet, first servo motor, second servo motor, camera, light filling lamp, electrical heating stick, semiconductor refrigeration piece, second ultraviolet disinfection lamp, temperature sensor, second electromagnet, touch entrance guard switch.

Further, the upper end of shielding plate leading flank is provided with the protrusion post, be provided with the holding chamber that extends to in the shielding plate in the protrusion post, the holding intracavity is provided with the blind bolt, the one end that the blind bolt stretches out the protrusion post is provided with the fixture block, the blind bolt is kept away from the one end of fixture block is provided with the second backstop board, the one end that the shielding plate was kept away from to the protrusion post is provided with the second annular backstop platform to its inside extension, the second spring has been cup jointed on the blind bolt, the both ends of second spring are contradicted respectively on second backstop board and second annular backstop platform.

Furthermore, a sliding groove which is longitudinally extended and used for the protrusion column to slide is formed in the right door plate on the upper side of the window, and clamping grooves matched with the clamping blocks are formed in the right door plate corresponding to the upper end and the lower end of the sliding groove.

Furthermore, the inner side wall of the accommodating cavity is provided with a guide boss, and a fourth opening matched with the guide boss is formed in the second stop plate.

Further, a touch screen and a main switch are arranged on the front side wall of the box body corresponding to the bottom cavity, and the touch screen and the main switch are connected with the MCU chip.

Furthermore, a plurality of circular through holes are formed in the side wall, facing the culture cavity, of the housing.

Furthermore, the quantity of first sprocket with subassembly quantity is the same is placed to the culture dish, and a plurality of first sprockets link to each other with the second sprocket through same chain.

Further, the length of the first bump is smaller than the sum of the lengths of the second gap and the third gap.

Further, the MCU chip is connected with the mobile terminal through a wireless network provided by the wireless network module.

Compared with the prior art, the invention has the beneficial effects that:

1. the box body is divided into a storage cavity and a culture cavity through a longitudinal partition plate and is respectively used for placing a limited culture dish and performing separation culture on microorganisms, wherein a window is formed in a right door plate, and a shielding plate capable of moving up and down is arranged on the window, so that the shielding plate is opened only when the culture condition in the culture cavity needs to be observed or a light source needs to be provided for the culture cavity, and the shielding plate is closed only when the observation is not needed or the light transmission is not needed in the culture cavity, so that the requirement of different microorganisms on the growth environment is met;

2. according to the culture dish placing assembly in the culture cavity, the placing plate can move back and forth under the action of the cooperation of the first servo motor, the second chain wheel, the first chain wheel, the driving gear and the sliding block, so that the culture dish can be placed conveniently; moreover, a first electromagnet, a push shaft, a first magnet on the push shaft and a first lug are arranged in the fixed shaft for mounting the first chain wheel, the first lug is matched with the first notch and the second notch on the first chain wheel only when the push shaft drives the first lug to be inserted into the jack of the first rotating shaft, the first chain wheel is driven to rotate the driving gear, the first electromagnet can be selectively opened when the culture dish rack is used, the placing plate corresponding to the opened first electromagnet can move back and forth, and the placing plate for taking and placing the culture dish is not required to move, so that the flexibility for taking and placing the culture dish is improved;

3. according to the invention, the camera assembly capable of lifting is arranged on the rear side wall of the culture cavity, the camera and the light supplement lamp on the first poly-wedge belt are moved through the mutual matching of the second servo motor, the first poly-wedge belt wheel, the second poly-wedge belt wheel, the first poly-wedge belt, the first belt wheel, the second belt wheel and the belt, and the camera and the light supplement lamp are moved to the upper side of the placing plate needing to observe the culture condition according to the requirement, so that the condition in the culture cavity can be conveniently collected;

4. the temperature in the culture cavity is reasonably adjusted by matching the MCU chip, the temperature sensor, the electric heating rod and the semiconductor refrigerating sheet, so that the temperature in the culture cavity is effectively ensured to be constant;

5. the MCU chip is used as a central control chip, receives a control instruction input by the touch screen, can receive data information of the temperature sensor and the camera, simultaneously controls corresponding execution components to work, and is connected with the mobile terminal through a wireless network provided by the wireless network module, so that automatic control and remote data sharing transmission are facilitated;

in a word, the invention has the advantages of convenient use, multiple functions, strong flexibility and convenient temperature adjustment.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the mechanism of the present invention without the left and right door panels installed;

FIG. 3 is a schematic longitudinal cross-sectional view of a right door panel window according to the present invention;

FIG. 4 is an enlarged view of part C of the present invention;

FIG. 5 is a schematic cross-sectional view of a right door panel at a window according to the present invention;

FIG. 6 is a schematic longitudinal cross-sectional view of a projecting post portion of the present invention;

FIG. 7 is a schematic longitudinal sectional view of the case of the present invention taken along the lifting space;

FIG. 8 is a schematic longitudinal sectional view from left to right of the case of the present invention;

FIG. 9 is a schematic longitudinal section of the case of the present invention;

FIG. 10 is an enlarged view of portion A of FIG. 8;

FIG. 11 is a schematic view showing the fixed shaft, the pushing shaft and the first sprocket in a disassembled state;

FIG. 12 is a right side view of the stationary shaft of FIG. 11;

FIG. 13 is a front view of the stationary shaft of FIG. 12;

FIG. 14 is a right side elevational view of the push shaft of FIG. 11;

FIG. 15 is a right side elevational view of the first sprocket of FIG. 11;

FIG. 16 is a front view of the first shaft of the present invention;

FIG. 17 is a left side view of the first shaft of the present invention;

FIG. 18 is a schematic view showing the structure of a slide rail on the left side wall of a culture chamber;

fig. 19 is a partial cross-sectional view of portion B of fig. 18.

In the figure, 1, a box body, 101, a bottom cavity, 1011, a first servo motor, 1012, a second chain wheel, 1013, a chain, 102, a storage cavity, 1021, a first ultraviolet disinfection lamp, 103, a culture cavity, 1031, a cover, 13011, a circular through hole, 1032, a slide rail, 10321, an upper slide plate, 10322, a lower slide plate, 10323, a first boss, 10324, a first stop plate, 10325, a second boss, 10326, a strip hole, 1033, a placing plate, 10331, a transverse plate, 10332, a slide block, 10341, a driving gear, 10342, a first rotating shaft, 43, a fixed shaft, 10344, a first chain wheel, 10345, a first electromagnet, 10346, a pushing shaft, 10347, a first magnet, 10348, a first bump, 10349, a first annular stop table, 103410, a first gap, 103411, a second gap, 103412, a jack, 103413, a third gap, 414, a first spring, 104, a left door panel, 103105, a right door panel, a glass sealing plate, 10321, 10521 and a sealing plate, 10521, 10522. the accommodating cavity, 10523, the draw bolt, 10524, the fixture block, 10525, the second backstop board, 10526, the second ring-shaped stop table, 10527, the second spring, 10528, the spout, 10529, the draw-in groove, 105210, the direction boss, 106, the longitudinal baffle, 1061, the cavity, 1071, first polywedge pulley, 1072, the second polywedge pulley, 1073, the second servo motor, 1074, first polywedge belt, 1075, the first belt pulley, 1076, the second belt pulley, 1077, the belt, 1078, the camera, 1079, the light filling lamp, 108, the second ultraviolet disinfection lamp, 109, temperature sensor, 2, the electrical heating stick, 3, the semiconductor refrigeration piece, 4, the control circuit board, 401, the MCU chip, 402, the wireless network module, 5, the second magnet, 6, the touch screen, 7, the master switch, 8, the second electromagnet, 9, the touch switch.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

As shown in fig. 1 to 19, a multifunctional aseptic constant temperature incubator for separating microorganisms comprises a case body 1 with a base, wherein the case body 1 is provided with a bottom cavity 101 for mounting electric elements, a storage cavity 102 for storing culture dishes and a culture cavity 103 for culturing microorganisms, the front side of the case body 1 is hinged with a left door panel 104 and a right door panel 105 which respectively correspond to the storage cavity 102 and the culture cavity 103, and the storage cavity 102 and the culture cavity 103 are separated by a longitudinal partition 106;

the storage cavity 102 is divided into at least two placing units by a partition board, and a first ultraviolet disinfection lamp 1021 is arranged on the side wall of each placing unit;

the culture chamber 103 is provided with a plurality of culture dish placing components capable of moving back and forth along the longitudinal direction of the culture chamber, two housings 1031 are symmetrically arranged on the rear side wall of the culture chamber 103, a certain distance is reserved between the two housings 1031 to form a lifting space, and a camera shooting component capable of lifting is arranged in the lifting space.

As shown in fig. 2, 8, 11-19, the culture dish placement assembly comprises a sliding rail 1032 symmetrically arranged on the left and right side walls of the culture chamber 103, and a placement plate 1033 capable of moving back and forth along the sliding rail 1032;

the sliding rail 1032 comprises an upper sliding plate 10321 extending along the direction of the rear wall of the culture chamber 103 and a lower sliding plate 10322 positioned on the lower side of the upper sliding plate 10321, the upper sliding plate 10321 and the lower sliding plate 10322 are fixed on the side wall of the culture chamber 103 through bolts, and the rear end of the sliding rail 1032 abuts against the side edge of the housing 1031; a first boss 10323 extending downwards is arranged at one end of the upper sliding plate 10321 away from the end connected with the side wall of the culture chamber 103, a first stop plate 10324 extending downwards is arranged at one end of the upper sliding plate 10321 facing the opening of the culture chamber 103, the height of the first stop plate 10324 is smaller than that of the first boss 10323, so that the lower end of the first boss 10323 protrudes out of the first stop plate 10324, a second boss 10325 extending upwards is arranged at one end of the lower sliding plate 10322 away from the end connected with the side wall of the culture chamber 103, the first boss 10323 and the second boss 10325 are positioned on the same vertical plane, a gap for the placement plate 1033 to pass through is arranged between the first boss 10323 and the second boss 10325, and a strip-shaped hole 10326 is arranged at one end of the lower sliding plate 10322 positioned at the left side wall of the culture chamber 103 facing the opening of the culture chamber 103103;

an accommodating space is formed between the upper slide plate 10321 and the lower slide plate 10322;

the placement board 1033 comprises a transverse board 10331 and sliding blocks 10332 symmetrically arranged at the left and right ends of the transverse board 10331, the sliding blocks 10332 are embedded in the accommodating space between the upper side board 10321 and the lower side board 10322, the transverse board 10331 passes through the gap, the thickness of the sliding blocks 10332 is not more than the distance between the lower side surface of the first stop board 10324 and the upper side surface of the lower sliding board 10322, and the rear end of the sliding blocks 10332 extends upwards to form a third stop board, so that the first stop board 10324 is matched with the third stop board to prevent the sliding blocks 10332 from escaping from the accommodating space when sliding forwards, and the lower side surface of the sliding block 10332 at the left end of the transverse board 10331 is provided with teeth;

a driving gear 10341 is arranged on the lower side of the lower slide plate 10322 on the left side wall of the culture chamber 103, one end of the driving gear 10341 extends into the strip-shaped hole 10326, and the driving gear 10341 is meshed with teeth on the lower side of the slide block 10332 on the left end of the transverse plate 10331, so that the slide block 10332 can be driven to slide back and forth along the slide rail 1032 during the rotation of the driving gear 10341;

the driving gear 10341 is rotatably connected to the longitudinal partition 106 through a first rotating shaft 10342, the longitudinal partition 106 has a hollow chamber 1061, and an end of the first rotating shaft 10342, which is far away from the driving gear 10341, extends into the chamber 1061;

a hollow fixed shaft 10343 is arranged in the chamber 1061 corresponding to the first rotating shaft 10342, the left end of the fixed shaft 10343 is spaced from the first rotating shaft 10342 by a distance of 3-5mm, the distance is set so that the fixed shaft 10343 does not affect the rotation of the first rotating shaft 10342, the fixed shaft 10343 is provided with a first chain wheel 10344 rotating towards one end of the first rotating shaft 10342, the right end of the fixed shaft 10343 is provided with a first annular stop table 10349 extending towards the inside thereof, the fixed shaft 10343 is provided with a first electromagnet 10345 fixed on the inner side wall of the longitudinal partition 106, the right side of the first electromagnet 10345 is provided with a push shaft 10346, the left end of the push shaft 10346 is provided with a first magnet 10347, the first magnet 10347 is fixed on the end of the push shaft 10346 by an adhesive or a screw, the first electromagnet 10345 has opposite magnetism to the first magnet 10347 itself after being energized, the first magnet 10345 can repel the first magnet 10347 after being energized, so as to push the push shaft 10346 to slide toward the first rotation shaft 10342, a first protrusion 10348 is disposed on a circumferential surface of a right end of the push shaft 10346, a first spring 103414 is sleeved on an outer circumference of the push shaft 10346, the first spring 103414 is an elastic spring, two ends of the first spring 103414 respectively abut against the first magnet 10347 and the first annular stop table 10349, so that when the first electromagnet 10345 loses magnetism after being de-energized, the first spring 103414 slides back into the fixed shaft 10343 under the elastic force of the first spring 103414, a first notch 410 corresponding to the first protrusion 10348 is disposed at a position of the right end of the fixed shaft 10343 corresponding to the first annular stop table 10349, and when the push shaft 10346 is accommodated in the fixed shaft 10343, the first protrusion 10348 is embedded in the first notch 103410;

the first chain wheel 10344 is provided with a stepped through hole, the right end of the fixed shaft 10343 is connected with a large-diameter hole of the stepped through hole through a bearing, the diameter of a small-diameter hole of the stepped through hole is the same as the outer diameter of the push shaft 10346, a second notch 103411 corresponding to the first notch 103410 is formed in the hole wall of the small-diameter hole of the stepped through hole, the left end of the first rotating shaft 10342 is provided with an insertion hole 103412 for inserting the push shaft 10346, and the hole wall of the insertion hole 103412 is provided with a third notch 103413 for inserting the first boss 10348; therefore, after the first electromagnet 10345 is energized and has magnetism, in the process of pushing the push shaft 10346 to slide, the first bump 10348 slides out of the first notch 103410, slides through the second notch 103411 and then is clamped in the third notch 103413, at this time, part of the first bump 10348 is clamped in the second notch 103411, that is, the first bump 10348 is simultaneously clamped in the second notch 103411 and the third notch 103413, so that the first sprocket 10344 is driven to rotate by the first shaft 10342 in the rotation process;

a first servo motor 1011 is fixed on the upper side wall of the bottom cavity 101, a second chain wheel 1012 is connected to the output shaft end of the first servo motor 1011, the second chain wheel 1012 is connected with the first chain wheel 10344 through a chain 1013, so that the first chain wheel 10344 is driven to rotate by the second chain wheel 1012 and the chain 1013 during the operation of the first servo motor 1011; the number of the first chain wheels 10344 is the same as that of the culture dish placing assemblies, and a plurality of the first chain wheels 10344 are connected with the second chain wheel 1012 through the same chain 1013; during the rotation of the second sprocket 1012, all the first sprockets 10344 in the chamber 1061 are rotated by the chain 1013, and only the first rotating shaft 10342 corresponding to the energized first electromagnet 10345 is rotated due to the cooperation between the first electromagnet 10345 and the first magnet 10347 in each fixed shaft 10343;

the camera shooting assembly comprises a first multi-wedge belt wheel 1071 arranged between two housings 1031 through a shaft, a second multi-wedge belt wheel 1072 positioned in the bottom cavity 101 and corresponding to the first multi-wedge belt wheel 1071, and a second servo motor 1073, wherein the first multi-wedge belt wheel 1071 is connected with the second multi-wedge belt wheel 1072 through a first multi-wedge belt 1074, a first belt pulley 1075 is arranged on the shaft of the second multi-wedge belt wheel 1072, a second belt pulley 1076 is arranged on an output shaft of the second servo motor 1073, the first belt pulley 1075 is connected with the second belt pulley 1076 through a belt 1077, and a camera 1078 and a light supplement lamp 1079 are arranged on the side surface of the first multi-wedge belt 1074 facing the culture cavity 103; in the operation process of the second servo motor 1073, the second belt pulley 1076 is driven to rotate, the second belt pulley 1076 drives the first belt pulley 1075 to rotate through a belt 1077, the first belt pulley 1075 drives the second multi-wedge belt pulley 1072 to rotate through a shaft, the second multi-wedge belt pulley 1072 drives the first multi-wedge belt pulley 1072 to rotate through a first multi-wedge belt 1074, a camera 1078 and a light supplement lamp 1079 are driven to move in the rotation process of the first multi-wedge belt 1072, the camera 1078 shoots image information of the environment inside the culture cavity 103, the light supplement lamp 1079 is turned on to illuminate the interior of the culture cavity 103, and the definition of pictures shot by the camera 1078 is improved;

a window is arranged on the right door plate 105, one end of the window facing the culture chamber 103 is provided with a sealing glass 1051, and one end of the sealing glass 1051 opposite to the culture chamber 103 is provided with a shielding plate 1052 capable of moving up and down; therefore, after the right door plate 105 is closed, the culture cavity 103 is a totally-enclosed space, and when the shielding plate 1052 is closed, the culture cavity 103 is light-tight;

the electric heating rods 2 and the semiconductor refrigerating pieces 3 are arranged on the rear side wall of the culture chamber 103 corresponding to the housing 1031 in a staggered manner from top to bottom; the electric heating rod 2 is started to heat the culture cavity 103, and the semiconductor refrigerating sheet 3 is started to cool the culture cavity 103; the left side wall and the right side wall of the culture cavity 103 are respectively provided with a second ultraviolet disinfection lamp 108 and a temperature sensor 109; two door second magnets 5 are arranged at one end, facing the opening of the box body 1, of the longitudinal partition plate 106, a second electromagnet 8 corresponding to the second magnets 5 is arranged on the left door panel 104 and the right door panel 105, the magnetism of the second electromagnet 8 after the second electromagnet is electrified is opposite to that of the second magnet 5, so that the left door panel and the right door panel are closed, the second electromagnet 8 is connected with a touch access control switch 9, the touch access control switch 9 has a fingerprint identification function and a password verification function, only authorized personnel input correct fingerprint information or passwords, and the first electromagnet 8 is powered off;

a control circuit board 4 is installed in the bottom cavity 101, an MCU chip 401 and a wireless network module 402 connected with the MCU chip 401 are installed on the control circuit board 4, a signal input end of the MCU chip 401 is connected with the temperature sensor 109, and a signal output end of the MCU chip 401 is connected with a first ultraviolet disinfection lamp 1021, a first electromagnet 10345, a first servo motor 1011, a second servo motor 1073, a second servo motor, a camera 1078, a light supplement lamp 1079, an electric heating rod 2, a semiconductor refrigeration sheet 3, a second ultraviolet disinfection lamp 108, the temperature sensor 109, a second electromagnet 8 and a touch access switch 9; in order to facilitate data storage, a storage module connected to the MCU chip 401 is integrated on the control circuit board 4 for storing set parameter information and image information captured by the camera 1078, and in order to facilitate time recording, a clock module connected to the MCU chip 401 is integrated on the control circuit board 4.

As shown in fig. 3 to 5, a protruding column 10521 is disposed at an upper end of a front side surface of the shielding plate 1052, a receiving cavity 10522 extending into the shielding plate 1052 is disposed in the protruding column 10521, a pulling plug 10523 is disposed in the receiving cavity 10522, a fastening block 10524 is disposed at an end of the pulling plug 10523 extending out of the protruding column 10521, a second stopping plate 10525 is disposed at an end of the pulling plug 10523 away from the fastening block 10524, a second annular stopping table 10526 extending towards an interior of the shielding plate 1052 is disposed at an end of the protruding column 10521 away from the shielding plate 1052, a second spring 10527 is sleeved on the pulling plug 10523, the second spring 10527 is an elastic spring, two ends of the second spring 10527 abut against the second stopping plate 10525 and the second annular stopping table 10526, respectively, when no external force is applied, the pulling plug 1053 is completely embedded in the receiving cavity 10522 under the action of the second spring 10527, and the fastening block 10524 abuts against the protruding column 10521; a sliding groove 10528 which longitudinally extends and is used for the projecting column 10521 to slide is arranged on the right door panel 105 at the upper side of the window, and a clamping groove 10529 matched with the clamping block 10524 is arranged on the right door panel 105 corresponding to the upper end and the lower end of the sliding groove 10528; when the shielding plate 1052 is in a closed state, the clamping block 10524 is clamped in the clamping groove 10529 at the lower end, when the shielding plate 1052 needs to be opened, the clamping block 10524 is pulled to enable the clamping block 10524 to be separated from the clamping groove 10529, then the clamping block 10524 is pulled upwards to enable the protruding column 10521 to slide along 10528, when the shielding plate 1052 slides to the uppermost end, the clamping block 10524 is loosened, and the clamping block 10524 is clamped in the clamping groove 10529 at the upper end, so that the shielding plate 1052 is fixed.

The inner side wall of the accommodating cavity 10522 is provided with a guide boss 105210, the second stop plate 10525 is provided with a fourth opening matched with the guide boss 105210, and the guide boss 105210 is matched with the fourth opening to limit the pulling bolt 1053, so that the pulling bolt 1053 is prevented from rotating and sliding up and down with the pulling fixture block 10524 unchanged.

A touch screen 6 and a main switch 7 are arranged on the front side wall of the box body 1 corresponding to the bottom cavity 101, the touch screen 6 and the main switch 7 are connected with the MCU chip 401, the main switch 7 is a button switch, and after the control circuit board 4 is powered on, only corresponding power utilization parts connected with the control circuit board 4 are powered on after the main switch 7 is turned on, and the whole incubator is started; the touch screen 6 is used as an input end of a control instruction, a user can input upper and lower limits of temperature thresholds required for starting the electric heating rod 2 and the semiconductor refrigerating sheet 3 at the culture temperature in the culture cavity 103 through the touch screen 6, the temperature sensors 109 monitor the temperature in the culture cavity 103 in real time, after the monitored temperatures are transmitted to the MCU chip 401 by the plurality of temperature sensors 109, the MCU chip 401 calculates the average temperature, when the average temperature is lower than the set lower threshold, the MCU chip 401 sends a starting instruction to the electric heating rod 2, the electric heating rod 2 supplies heat to the culture cavity 103 after being started, and when the temperature in the culture cavity 103 reaches the upper threshold, the MCU chip 401 sends a closing instruction to the electric heating rod 2; similarly, when the temperature in the culture cavity 103 is higher than the upper limit of the threshold value required by the starting of the semiconductor chilling plate 3, the MCU chip 401 sends a starting instruction to the semiconductor chilling plate 3, the semiconductor chilling plate 3 is started to cool the culture cavity 103, and when the temperature in the culture cavity 103 is reduced to the lower limit of the threshold value, the MCU chip 401 sends a closing instruction to the semiconductor chilling plate 3; in specific implementation, the upper and lower limits of the temperature threshold required by the opening and closing of the electric heating rod 2 and the upper and lower limits of the temperature threshold required by the opening and closing of the semiconductor chilling plate 3 can be set to be different, and the selective opening of the electric heating rod 2 and the semiconductor chilling plate 3 can also be set according to seasons.

The side wall of the housing 1031 facing the culture chamber 103 is provided with a plurality of circular through holes 10311, and the arrangement of the circular through holes 10311 increases the exchange efficiency between the culture chamber 103 and the temperature in the housing 1031 by utilizing the fluidity of air molecules.

The length of the first bump 10348 is smaller than the sum of the lengths of the second notch 103411 and the third notch 103413, so that when the right portion of the first bump 10348 is completely clamped into the third notch 103413, the first bump 10348 is completely separated from the first notch 103410, and the second sprocket 1012 and the first rotating shaft 10342 can be ensured to rotate smoothly.

The MCU chip 401 is connected with the mobile terminal through a wireless network provided by the wireless network module 402, and a user can be connected with the MCU chip 401 through the mobile terminal to set and control remote parameters and look up image information.

In the specific implementation of the present invention, a control program is written in the MCU chip 401, all the dish placement components in the culture chamber 103 have different codes, and each of the first electromagnets 10345 corresponding to each dish placement component individually sends a control signal, when a user inputs a command to pick and place a dish on the placement board 1033 of a certain dish placement component through the touch screen 6 or the mobile phone terminal, the MCU chip 401 first sends a power-off command to the second electromagnet 8, so as to open the left and right door panels, and then the first electromagnet 10345 in the fixed shaft 10343 corresponding to a certain dish component that needs to pick and place a dish is powered on with the same polarity as the first electromagnet 10347, pushes the corresponding push shaft 10346 to be inserted into the corresponding insertion hole 103412 in the first rotating shaft 10342, and then the MCU chip 401 sends an operation command to the first servo motor 1011 to drive the corresponding placement board 1033 to move outward, when the third baffle collides with the first stopper board 10324, that is, after the first servo motor 1011 runs for a certain distance, the first servo motor 1011 stops running, and after the culture dish taking and placing is finished, an end instruction is input through the touch screen 601 or the mobile phone terminal, at this time, the first servo motor 1011 rotates in the reverse direction to store the placing board 1033 in the culture chamber 103, then the first servo motor 1011 stops running, the first electromagnet 10345 loses magnetism when power is off, and the push shaft 10346 retracts into the fixed shaft 10343 under the elastic force of the first spring 103414; similarly, when the left and right door panels are in a closed state and a culture condition in the culture chamber 103 needs to be obtained, video acquisition information is input through the touch screen 601 and is input through the mobile phone terminal, the second servo motor 1073 drives the camera 1078 and the light supplement lamp 1079 to move up and down through the first V-ribbed belt 1074, and captured video information is processed by the MCU chip 401 and then stored in the storage module and is displayed through the touch screen 601 or the mobile phone terminal.

In the invention, the left and right door panels are matched with the second electromagnet 8 and the second magnet 5 by adopting the touch access switch 9 with fingerprint identification and password verification functions, so that the left and right door panels can be opened only under the operation of an authenticated manager, and the security and confidentiality are good; through the matching of the first servo motor 1011, the second chain wheel 1012, the second chain wheel 10344, the fixed shaft 10343, the push shaft 10346, the first electromagnet 10345, the first magnet 10347 and the first rotating shaft 10342, the selective movement of a plurality of culture dish assemblies is realized, only the placing plate 1033 in the culture dish assembly which needs to take and place the culture dish moves, and the management of the culture dish and the culture dish placing operation are facilitated; the storage cavity 102 and the culture cavity 103 are respectively sterilized before the culture operation through the first ultraviolet disinfection lamp 1021 and the second ultraviolet disinfection lamp 108, so that the sterile environment in the culture cavity 103 is effectively maintained, and a good culture environment is provided for the microbial culture in the culture cavity 103; in addition, the automatic and intelligent remote control of the incubator is realized through the MCU chip 401 and the wireless network module 402 which are integrated in the control circuit board 4.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a multi-functional microorganism separation is with aseptic constant temperature incubator, is including the box that has the base, the box has the end chamber that is used for installing electrical components, is used for the storage chamber of storage culture dish and is used for cultivateing the cultivation chamber of microorganism, the front side of box articulates there is left door plant and right door plant that corresponds with storage chamber and cultivation chamber respectively, the storage chamber forms its characterized in that with cultivating the chamber by the longitudinal baffle partition:

the storage cavity is divided into at least two placing units by partition plates, and a first ultraviolet disinfection lamp is arranged on the side wall of each placing unit;

the culture chamber is longitudinally provided with a plurality of culture dish placing assemblies capable of moving back and forth, the rear side wall of the culture chamber is symmetrically provided with two housings, a certain distance is reserved between the two housings to form a lifting space, and a camera assembly capable of lifting is arranged in the lifting space;

a hollow fixed shaft is arranged in the cavity corresponding to the first rotating shaft, the left end of the fixed shaft has a certain distance with the first rotating shaft, a first chain wheel is arranged at one end of the fixed shaft, which faces the first rotating shaft, in a rotating manner, a first annular stop table extending towards the inside is arranged at the right end of the fixed shaft, a first electromagnet fixed on the inner side wall of the longitudinal clapboard is arranged in the fixed shaft, a push shaft is arranged on the right side of the first electromagnet, the left end of the push shaft is provided with a first magnet, the magnetism of the first electromagnet after being electrified is opposite to the magnetism of the first magnet, a first bump is arranged on the circumferential surface of the right end of the push shaft, a first spring is sleeved on the periphery of the push shaft, two ends of the first spring respectively abut against the first magnet and the first annular stop table, and a first notch matched with the first convex block is formed in the position, corresponding to the first annular stop table, of the right end of the fixed shaft;

the first chain wheel is provided with a stepped through hole, the right end of the fixed shaft is connected into a large-diameter hole of the stepped through hole through a bearing, the aperture of a small-diameter hole of the stepped through hole is the same as the outer diameter of the push shaft, a second notch corresponding to the first notch is formed in the hole wall of the small-diameter hole of the stepped through hole, the left end of the first rotating shaft is provided with an insertion hole for the push shaft to insert, and a third notch for the first boss to insert is formed in the hole wall of the insertion hole; the length of the first bump is smaller than the sum of the lengths of the second gap and the third gap;

the camera shooting assembly comprises a first multi-wedge belt wheel arranged between two housings through a shaft, a second multi-wedge belt wheel positioned in the bottom cavity and corresponding to the first multi-wedge belt wheel, and a second servo motor, wherein the first multi-wedge belt wheel is connected with the second multi-wedge belt wheel through a first multi-wedge belt;

the rear side wall of the culture cavity corresponding to the housing is provided with electric heating rods and semiconductor refrigerating sheets in a staggered manner from top to bottom;

two door second magnets are arranged at one end, facing the opening of the box body, of the longitudinal partition plate, second electromagnets corresponding to the second magnets are arranged on the left door panel and the right door panel, the magnetism of the second electromagnets after being electrified is opposite to the magnetism of the second magnets, and the second electromagnets are connected with a touch entrance guard switch;

install control circuit board in the end intracavity, control circuit board last install MCU chip and with the wireless network module that the MCU chip is connected, the signal input part of MCU chip with temperature sensor links to each other, the signal output part of MCU chip connects first ultraviolet disinfection lamp, first electromagnet, first servo motor, second servo motor, camera, light filling lamp, electrical heating rod, semiconductor refrigeration piece, second ultraviolet disinfection lamp, temperature sensor, second electromagnet, entrance guard touch switch.

2. The multifunctional aseptic thermostatic incubator for microbe separation according to claim 1, characterized in that: the upper end of the front side face of the shielding plate is provided with a protruding column, a containing cavity extending into the shielding plate is formed in the protruding column, a pull bolt is arranged in the containing cavity, one end, extending out of the protruding column, of the pull bolt is provided with a clamping block, the pull bolt is far away from the clamping block, one end, far away from the shielding plate, of the protruding column is provided with a second annular stopping table extending towards the interior of the protruding column, a second spring is sleeved on the pull bolt, and two ends of the second spring are abutted to the second stopping table and the second annular stopping table respectively.

3. The multifunctional aseptic thermostatic incubator for microbe separation according to claim 2, characterized in that: the right door plate on the upper side of the window is provided with a longitudinally extending sliding groove for the protruding column to slide, and the right door plate corresponding to the upper end and the lower end of the sliding groove is provided with a clamping groove matched with the clamping block.

4. The multifunctional aseptic thermostatic incubator for microbe separation according to claim 2, characterized in that: the inner side wall of the accommodating cavity is provided with a guide boss, and a fourth opening matched with the guide boss is formed in the second stop plate.

5. The multifunctional aseptic thermostatic incubator for microbe separation according to claim 1, characterized in that: and a touch screen and a main switch are arranged on the front side wall of the box body corresponding to the bottom cavity, and the touch screen and the main switch are connected with the MCU chip.

6. The multifunctional aseptic constant-temperature incubator for microbial isolation according to claim 1, wherein: the side wall of the housing facing the culture cavity is provided with a plurality of circular through holes.

7. The multifunctional aseptic constant-temperature incubator for microbial isolation according to claim 1, wherein: the number of the first chain wheels is the same as that of the culture dish placing assemblies, and the first chain wheels are connected with the second chain wheels through the same chain.

8. The multifunctional aseptic thermostatic incubator for microbe separation according to claim 1, characterized in that: the MCU chip is connected with the mobile terminal through a wireless network provided by the wireless network module.