CN111849729A - Nano phase change constant temperature culture dish and manufacturing method thereof - Google Patents
Nano phase change constant temperature culture dish and manufacturing method thereof Download PDFInfo
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- CN111849729A CN111849729A CN202010001183.4A CN202010001183A CN111849729A CN 111849729 A CN111849729 A CN 111849729A CN 202010001183 A CN202010001183 A CN 202010001183A CN 111849729 A CN111849729 A CN 111849729A
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- 230000008859 change Effects 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 239000000463 material Substances 0.000 claims abstract description 52
- 239000002775 capsule Substances 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims description 27
- 238000007789 sealing Methods 0.000 claims description 20
- 239000004033 plastic Substances 0.000 claims description 17
- 229920003023 plastic Polymers 0.000 claims description 17
- 238000001746 injection moulding Methods 0.000 claims description 16
- 239000011229 interlayer Substances 0.000 claims description 14
- 230000007704 transition Effects 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 7
- PMHQVHHXPFUNSP-UHFFFAOYSA-M copper(1+);methylsulfanylmethane;bromide Chemical compound Br[Cu].CSC PMHQVHHXPFUNSP-UHFFFAOYSA-M 0.000 claims description 6
- 239000012782 phase change material Substances 0.000 claims description 6
- 238000002844 melting Methods 0.000 claims description 4
- 230000008018 melting Effects 0.000 claims description 4
- 231100000252 nontoxic Toxicity 0.000 claims description 4
- 230000003000 nontoxic effect Effects 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 238000003466 welding Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 230000010412 perfusion Effects 0.000 claims description 2
- 238000003672 processing method Methods 0.000 claims description 2
- 239000005020 polyethylene terephthalate Substances 0.000 claims 5
- 229920000139 polyethylene terephthalate Polymers 0.000 claims 5
- 239000004743 Polypropylene Substances 0.000 claims 4
- 239000004417 polycarbonate Substances 0.000 claims 4
- 239000004698 Polyethylene Substances 0.000 claims 3
- -1 polypropylene Polymers 0.000 claims 3
- 239000012994 photoredox catalyst Substances 0.000 claims 2
- 239000004831 Hot glue Substances 0.000 claims 1
- 239000002088 nanocapsule Substances 0.000 claims 1
- 239000002086 nanomaterial Substances 0.000 claims 1
- 229920000515 polycarbonate Polymers 0.000 claims 1
- 229920001155 polypropylene Polymers 0.000 claims 1
- 239000010410 layer Substances 0.000 abstract description 12
- 238000003860 storage Methods 0.000 abstract description 8
- 239000002356 single layer Substances 0.000 abstract description 4
- 239000012071 phase Substances 0.000 description 31
- 238000000034 method Methods 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- 239000007787 solid Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 2
- 230000002277 temperature effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 239000004836 Glue Stick Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention provides a nanometer phase-change constant-temperature culture dish, which improves the single-layer structure of the traditional culture dish to manufacture a double-layer culture dish in a hollow sandwich structure form, injects a nanometer phase-change constant-temperature capsule material into the middle of a sandwich layer, and then is sealed to manufacture the nanometer phase-change constant-temperature culture dish. According to the culture dish, when the ambient temperature rises, the nano phase change capsule material can absorb external heat for storage, and when the ambient temperature is lower than the phase change temperature, the nano phase change capsule material can release heat outwards, so that the relative stability of the temperature in the culture dish within a certain time range is maintained. The invention also provides a manufacturing method of the nano phase change constant temperature culture dish.
Description
Technical Field
The invention relates to the technical field of production, manufacture and application of microorganism culture vessels, in particular to a nanometer phase change constant temperature culture vessel and a manufacturing method thereof.
Background
At present, the microorganism culture dish commonly used in the biological test culture room generally adopts the traditional glass culture dish or plastic culture dish with a cover on the upper part, the culture dishes have the characteristics of low manufacturing cost and convenient use, but the heat is not preserved, the growth of the culture in the culture dish is easily influenced by the change of the external environment, especially under the condition that the constant temperature guarantee condition is lost in a short period such as continuous heating or field transfer in a constant temperature box and the culture room due to power failure in winter, irreparable accidental loss can be caused to the microorganism or biological cell which requires constant temperature culture, and the practical problem which can be generally encountered and needs to be solved by a microorganism culture department is provided.
Disclosure of Invention
The invention provides a nanometer phase-change constant-temperature culture dish, which improves the single-layer structure of the traditional culture dish to manufacture a hollow sandwich structure type double-layer culture dish, a gap is reserved in the middle of a sandwich layer to be used as a storage layer for containing a nanometer phase-change constant-temperature capsule material, and a certain amount of liquid nanometer phase-change constant-temperature capsule material is injected into the gap of the storage layer and then sealed to manufacture the nanometer phase-change constant-temperature culture dish. This kind of culture dish, when ambient temperature is higher than phase transition temperature, the nanometer phase transition constant temperature material in the storage layer can absorb external heat and store the buffering in the culture dish, the relative stability of temperature in keeping the culture dish in certain time, when ambient temperature is less than phase transition temperature, nanometer phase transition capsule will outwards release the heat, delay the reduction of temperature in the culture dish, temperature keeps the relative stability in making the culture dish, the culture effect of culture normal growth in the phase transition constant temperature protection culture dish has been played, the intention is novel, the characteristics that the practicality is strong, certain popularization and application is worth. The invention also provides a manufacturing method of the nano phase change constant temperature culture dish.
The invention realizes the aim through the following technical scheme, and the nano phase-change constant-temperature culture dish consists of a culture dish, a culture dish cover, a nano phase-change constant-temperature capsule material and a liquid injection sealing port, and is characterized in that: the culture dish and the culture dish cover are manufactured into a hollow sandwich structure mode, and nano phase-change constant-temperature capsule materials are stored in the sandwich space.
The culture dish is made into a round shape, the diameter is 40-100mm, the height is 10-25mm, the wall thickness of an inner interlayer material and an outer interlayer material is 0.5-1 mm, the width of an interlayer gap is 2-5mm, the culture dish is made of nontoxic transparent PP or PET, PE and PC plastics by injection molding, 2-5% of nano aluminum nitride heat-conducting powder materials are doped in the injection molding process, and the heat conductivity coefficient of the plastic materials is improved by 20-40%.
The culture dish cover is matched with a culture dish to be manufactured into a circular shape, the inner diameter of the culture dish cover is 0.5-2 mm larger than the outer diameter of the culture dish to be used in a matched mode, the diameter of the culture dish cover is 46-106mm, the height of the culture dish cover is 10-25mm, the wall thickness of an inner interlayer material and an outer interlayer material is 0.5-1 mm, the width of an interlayer gap is 2-5mm, the culture dish cover is manufactured by adopting non-toxic transparent PP or PET, PE and PC plastic through injection molding, 2-5% of nano aluminum nitride heat-conducting powder material is doped in the injection molding process, and the heat conductivity.
The nanometer phase change constant temperature capsule material adopts a liquid nanometer capsule organic phase change material provided by Beijing phase change materials GmbH, the phase change material is formed by wrapping phase change nanometer material particles in a temperature range required by microbial culture in a 2-10 micron plastic capsule through a special processing technology and sealing, when the environmental temperature is higher than the phase change temperature of the nanometer phase change constant temperature capsule material, the phase change nanometer material in the micron capsule starts to melt and convert from a solid powder state to a liquid state after absorbing heat in a phase change process, and absorbs a large amount of heat to store in the conversion process, wherein the absorption capacity per ml is 280J, so that the rapid temperature rise in a culture dish is effectively prevented and slowed down; when the environmental temperature is lower than the temperature in the culture dish box, the nanometer phase change constant temperature capsule material starts a reverse phase change process, is converted from a liquid state to a solid state, gradually releases heat outwards in the conversion process, slows down the temperature reduction speed in the culture dish, keeps the temperature relatively stable within a certain time range, and plays a role in protecting cultures in the culture dish from being impacted by severe temperature change. The phase transition temperature of the nano phase transition constant temperature capsule material is manufactured according to the requirement of the temperature range of a specific culture, and can be selected between 20 ℃ and 45 ℃ to be customized by manufacturers.
The liquid injection sealing openings are formed in the upper side of the outer edge of the culture dish cover and the lower side of the edge of the culture dish, the aperture is 2-3mm, and the volume of the liquid nanometer phase change constant temperature capsule material injected into the interlayer gap of the culture dish and the culture dish cover is 10-30 ml.
The invention generates social benefits: the invention provides a nanometer phase-change constant-temperature culture dish, which improves the single-layer structure of the traditional culture dish to manufacture a hollow sandwich structure type double-layer culture dish, a gap is reserved in the middle of a sandwich layer to be used as a storage layer for containing a nanometer phase-change constant-temperature capsule material, and a certain amount of liquid nanometer phase-change constant-temperature capsule material is injected into the gap of the storage layer and then sealed to manufacture the nanometer phase-change constant-temperature culture dish. When the environment temperature is higher than the phase-change temperature, the nanometer phase-change constant-temperature material in the storage layer in the culture dish can absorb the external heat for storage and buffering, the relative stability of the temperature in the culture dish is kept within a certain time, and when the environment temperature is lower than the phase-change temperature, the nanometer phase-change capsule can release the heat outwards to delay the reduction of the temperature in the culture dish, so that the temperature in the culture dish is kept relatively stable, and the culture effect of the normal growth of microorganisms in the phase-change constant-temperature protection culture dish is achieved. The novel electric heating cooker has the characteristics of novel originality and strong practicability, and has certain popularization and application values. The invention also provides a manufacturing method of the nano phase change constant temperature culture dish.
Embodiments of the present invention will be described in detail with reference to the accompanying drawings
Drawings
FIG. 1 is a schematic view of the overall structure of the product of the present invention
FIG. 2 is the temperature curve diagram of the solid-liquid phase change of the nano phase change material of the product of the present invention
FIG. 3 is a schematic diagram showing the constant temperature effect of the product of the present invention during use
FIG. 4 is a schematic diagram showing the constant temperature effect of the product of the present invention during use
In the figure, a culture dish 1, a culture dish 2, a culture dish cover 3, a nanometer phase-change constant-temperature capsule material 4 and a liquid injection sealing port
Detailed description of the invention
Referring to fig. 1, the nano phase-change thermostatic culture dish of the invention comprises a culture dish 1, a culture dish cover 2, a nano phase-change thermostatic capsule material 3 and a liquid injection sealing port 4, and the production and processing method is described as follows with reference to fig. 1 and fig. 2:
1: and (3) injection molding to manufacture a culture dish 1 and a culture dish cover 2: loading a special injection mold for manufacturing the culture dish on an injection molding machine, uniformly doping nano aluminum nitride powder into PP plastic particles according to the proportion of 2-5%, and performing injection molding according to a conventional injection molding process flow to manufacture the inner and outer shells of the culture dish 1 and the culture dish cover 2;
2: ultrasonic welding: respectively melting and sealing the edges of the inner shell and the outer shell of the culture dish 1 and the culture dish cover 2 by using an ultrasonic plastic melting and welding machine to prepare complete finished products of the culture dish 1 and the culture dish cover 2;
3: pouring liquid nano phase change constant temperature capsule material 3: inserting a needle head of a perfusion machine into a culture dish 1 and a reserved liquid injection sealing port 4 of a culture dish cover 2, and injecting nano phase change constant-temperature capsule materials 3 into the box at a uniform speed until the box is full;
4: sealing the sealing port 4 for the injection liquid: and (3) filling the liquid nano phase-change constant-temperature capsule material 3, injecting the liquid nano phase-change constant-temperature capsule material into a sealing port by using a hot melt glue stick in a molten fluid state, sealing, naturally cooling, and solidifying to finish the manufacture of a finished product of the phase-change constant-temperature culture dish.
Referring to fig. 3 and 4, the using process and the constant temperature performance of the nano phase-change constant temperature culture dish of the invention are expressed as follows: referring to fig. 3, taking a bacteria culture dish with a phase transition temperature of 27 ℃ as an example, when the ambient temperature is lower than 25 ℃, the micron-sized phase transition capsule of the nano-phase transition thermostatic capsule material 3 is in a solid state, simultaneously releases latent heat stored in early stage, plays a role of heat preservation, when the environmental temperature exceeds 27 ℃ and rises sharply to be higher than 37 ℃, after about 10 minutes, the phase change capsule gradually melts into liquid after absorbing external heat, in the conversion process, each mI phase change material can absorb 280J latent heat to be stored, so that the constant temperature buffer effect is achieved, external heat cannot be rapidly conducted into the culture dish to influence the normal culture of microorganisms, when the outside temperature is reduced to below 27 ℃ again, the nano phase change thermostatic capsule material 3 enters a reverse phase change process from a liquid state to a solid state, latent heat stored in the nano phase change thermostatic capsule material is gradually released to the outside, and the temperature in the culture dish is kept relatively constant within a certain time range.
Referring to FIG. 4, the left vertical line 1 represents the heat transfer rate of a conventional glass culture dish, and it can be seen that when the ambient temperature rapidly rises to 37 ℃, the temperature in the culture dish rises to be substantially the same as the outside within a short time of 5-10 minutes; the vertical line 2 represents a culture dish made of plastic, and the temperature rise is slow due to poor heat-conducting property of the plastic, and the temperature in the culture dish gradually rises to 37 ℃ within 0.5 hour; the vertical line 3 represents the temperature characteristic curve of the nano phase change thermostatic culture dish, and due to the temperature stabilizing effect of the nano phase change thermostatic capsule material 3, the same temperature inside and outside the culture dish can be reached within 2 hours, so that the product of the invention has better relative constant temperature performance, and the quality of the product is improved compared with that of a single-layer glass culture dish and a plastic culture dish.
Claims (6)
1. The utility model provides a nanometer phase transition constant temperature culture dish, by culture dish (1), culture dish lid (2), nanometer phase transition constant temperature capsule material (3) and annotate liquid sealing port (4) and constitute its characterized in that: the culture dish (1) and the culture dish cover (2) are manufactured into a hollow sandwich structure mode, and nano phase-change constant-temperature capsule materials (3) are stored in the sandwich space.
2. The nano phase-change constant temperature culture dish of claim 1, wherein: the culture dish (1) is made into a round shape, the diameter is 40-100mm, the height is 10-25mm, the wall thickness of an inner interlayer material and an outer interlayer material is 0.5-1 mm, the width of an interlayer gap is 2-5mm, the culture dish is made of nontoxic transparent PP (polypropylene) or PET (polyethylene terephthalate), PE (polyethylene terephthalate) and PC (polycarbonate) plastics by injection molding, and 2-5% of nano aluminum nitride heat-conducting powder materials are doped in the injection molding process, so that the heat conductivity coefficient of a plastic material is improved.
3. The nano phase-change constant temperature culture dish of claim 1, wherein: the culture dish cover (2) is matched with the culture dish (1) to be manufactured into a circular shape, the inner diameter of the culture dish cover (2) is 0.5-2 mm larger than the outer diameter of the culture dish (1) for use, the diameter of the culture dish cover (2) is 46-106mm, the height of the culture dish cover is 10-25mm, the wall thickness of an inner interlayer material and an outer interlayer material is 0.5-1 mm, the width of an interlayer gap is 2-5mm, the culture dish cover is manufactured by adopting non-toxic transparent PP or PET, PE and PC plastic injection molding, 2-5% of nano aluminum nitride heat-conducting powder material is doped in the injection molding process, and the heat conductivity coefficient of the.
4. The nano phase-change constant temperature culture dish of claim 1, wherein: the nano phase-change thermostatic capsule material (3) is a liquid nano capsule organic phase-change material, the phase-change material is formed by wrapping phase-change nano material particles in a temperature range required by microbial culture in a 2-10 micron plastic capsule through a special processing technology and sealing, and the phase-change temperature of the nano phase-change thermostatic capsule material (3) is selected between 20 ℃ and 45 ℃ according to the specific culture temperature requirement.
5. The nano phase-change constant temperature culture dish of claim 1, wherein: the liquid injection sealing port (4) is arranged on the upper side of the outer edge of the culture dish cover (2) and the lower side of the edge of the culture dish (1), the aperture is 2-3mm, and the volume of the liquid nanometer phase change constant temperature capsule material (3) injected into the interlayer gap of the culture dish (1) and the culture dish cover (2) is 10-30 ml.
6. The nano phase-change constant-temperature culture dish according to claim 1, wherein the production and processing method is described as follows:
1) and (3) manufacturing a culture dish (1) and a culture dish cover (2) by injection molding: loading a special injection mold for manufacturing a culture dish on an injection molding machine, uniformly doping nano aluminum nitride powder into PP or PET, PE and PC plastic particles according to the proportion of 2-5%, and performing injection molding according to a conventional injection molding process flow to manufacture inner and outer shells of the culture dish (1) and the culture dish cover (2);
2) ultrasonic welding: respectively melting and sealing the edges of the inner shell and the outer shell of the culture dish (1) and the culture dish cover (2) by using an ultrasonic plastic melting and welding machine to prepare complete plastic finished products of the culture dish (1) and the culture dish cover (2);
3) pouring liquid nano phase change constant temperature capsule material (3): inserting the needle of the perfusion machine into a reserved liquid injection sealing port (4) of the culture dish (1) and the culture dish cover (2), and injecting liquid nano phase-change constant-temperature capsule materials (3) into the box at a uniform speed until the box is full of liquid nano phase-change constant-temperature capsule materials;
4) sealing the sealing port (4) for the injection liquid: and (3) after being filled with the liquid nano phase-change constant-temperature capsule material (3), pulling out the needle head of the filling machine, injecting a hot melt adhesive rod into the sealing port in a molten fluid state, sealing, naturally cooling, and solidifying to finish the manufacture of the finished product of the phase-change constant-temperature culture dish.
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| CN202010001183.4A CN111849729A (en) | 2020-01-02 | 2020-01-02 | Nano phase change constant temperature culture dish and manufacturing method thereof |
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