CN111830013B - Method for improving measurement accuracy of hormone concentration measurement equipment - Google Patents
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- CN111830013B CN111830013B CN202010599007.5A CN202010599007A CN111830013B CN 111830013 B CN111830013 B CN 111830013B CN 202010599007 A CN202010599007 A CN 202010599007A CN 111830013 B CN111830013 B CN 111830013B
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Abstract
The invention relates to the technical field of hormone concentration measurement methods, and discloses a measurement method for improving the detection accuracy of hormone concentration measurement equipment aiming at the problem of inaccurate measurement results caused by a calibration method in the existing hormone concentration equipment test, wherein the first hormone concentration measurement equipment is used for detecting a series of standard substances of hormone to be detected, recording data, and preparing a corresponding stable calibration rod according to the recorded data; the second hormone concentration measuring equipment collects information of the calibration rod and records data; and the second hormone concentration measuring equipment measures the acquisition value of the hormone to be measured, and the concentration of the hormone to be measured is calculated according to the second recorded data. The method collects the concentration values of a plurality of standard products, correspondingly prepares a plurality of calibration rods, calibrates the plurality of concentrations, calculates the concentrations by a piecewise linear approximation algorithm, prepares the calibration rods by adopting printing paper strips with gradually changed gray levels, keeps the stability of data and the consistency of the standard products by utilizing the corresponding relation between the collected values and the gray levels, and obviously improves the measurement accuracy.
Description
Technical Field
The invention relates to the technical field of hormone concentration measurement methods, in particular to a method for improving measurement accuracy of hormone concentration measurement equipment.
Background
The measurement accuracy of the existing hormone concentration test equipment is improved by calibrating the equipment when the concentration is measured, and the calibrating steps are as follows: a relational expression of absorption quantity and concentration is fitted by collecting a plurality of concentrations and corresponding absorption quantities, then a concentration value b of a certain concentration of the hormone to be measured on equipment to be determined is collected, a concentration value a of the same concentration of the hormone on standard equipment is collected as a standard value, then equipment difference is adjusted by taking a coefficient c ═ b/a as a calibration parameter, and the adjusted data is substituted into the relational expression to calculate the result, so that the accuracy of the measurement result of the same concentration of the same hormone on different equipment is achieved. However, as shown in fig. 3, since the collected data of different concentrations of the same hormone do not show linear changes, calibrating different concentrations of different devices by using the same calibration coefficient leads to poor data accuracy, thereby affecting inaccurate measurement results of hormone concentration measurement devices.
Disclosure of Invention
Aiming at the problem of inaccurate measuring result caused by a calibration method in the testing process of the conventional hormone concentration equipment, the invention aims to provide a measuring method for improving the detection accuracy of the hormone concentration measuring equipment.
The invention provides the following technical scheme:
a method of improving the measurement accuracy of a hormone concentration measurement device, comprising the steps of:
1) detecting a series of standard products with different concentrations of hormone to be detected by using first hormone concentration measuring equipment, and recording data, wherein the data comprises the concentration of the standard product, a plurality of times of acquisition values of the standard product with corresponding concentration and an average value of the acquisition values, and the average value is used as standard data of the corresponding concentration on the first hormone concentration measuring equipment;
2) correspondingly preparing stable calibration rods according to the acquisition values of different concentrations and different times in the step 1);
3) the second hormone concentration measuring equipment acquires information of the calibration rod as an acquired value of the corresponding concentration on the second hormone concentration measuring equipment in the step 1), and records data;
4) and (3) measuring the acquisition value of the hormone to be measured by the second hormone concentration measuring equipment, and calculating the concentration of the hormone to be measured by the recorded data in the step 3).
When the value of the standard substance is measured, the first hormone concentration measuring device and the second hormone concentration measuring device collect the same standard substance for multiple times, then the average collection value is calculated, the average collection value is compared with the average collection value measured by a blank sample when the concentration is 0 to obtain the absorption amount, and the concentration value of the hormone to be measured is calculated according to the absorption amount and the concentration of the corresponding standard substance.
Preferably, the method for detecting the series of standards of different concentrations of the hormone to be detected by the first hormone concentration measuring device comprises the following steps: the standard is reacted with a corresponding test strip rod, and then the test strip rod is measured by a first hormone concentration measuring device.
As a preference of the process of the invention, the preparation of the calibration rod in step 2) is as follows:
(1) preparing a color selecting paper strip: selecting a printing paper strip with the gray scale gradually changed and the length of 500mm as a color selecting paper strip; the color selection paper strip is provided with two parallel color bands extending along the length direction of the printing paper strip, wherein the color of one color band is CMYK00047, the color of the other color band is gradually changed from CMYK0007 at one end to CMYK000100 at the other end, and the color selection paper strip is provided with labels 1-119 distributed along the length direction, wherein the label 1 corresponds to one end of CMYK0007 in color, and the 119 corresponds to one end of CMYK000100 in color;
the corresponding relation between the labels and the color numbers on the color selection paper strip is as follows: y is str-x (str-end)/(119-1),
wherein y is a color number, x is a reference number, str is a color number str-0 xf4f4f4 corresponding to reference number 1, and end is a color number end-0 x221815 corresponding to reference number 119;
(2) determining the color number: finding the corresponding color number on the color selection paper strip according to the acquisition value recorded in the step 1), and determining the corresponding label;
(3) preparing a calibration rod: and (3) preparing the calibration rod by using the pure-color printing paper strips with the marks corresponding to the color numbers determined in the step (2).
Preferably, the method for acquiring information on the calibration bar by the second hormone concentration measuring device in the step 3) comprises the following steps: and the second hormone concentration measuring equipment acquires the gray value of the calibration rod prepared by the pure-color printing paper strip, obtains the acquired value of the concentration of the standard substance on the second hormone concentration measuring equipment, and records data.
Taking the measurement of the concentration of luteinizing hormone LH at 20miu/ml as an example, if the collection value on the first hormone concentration measurement device is 792.8, the corresponding color number is found on the printing paper slip by the least dichotomy by using the corresponding relationship between the collection value and the gray value, and the collection value is corresponding to the lower label, then the measurement result of the label on the first hormone concentration measurement device is 792.8, that is, the label represents the concentration of LH at 20 miu/ml; of course, taking the calibration bar prepared from the pure color printing paper strip with the color number corresponding to the label to test by a second hormone concentration measuring device represents that the acquired value corresponding to the device is used for obtaining the 20miu/ml concentration of LH.
As a preferable example of the method of the present invention, the method of calculating the concentration to be measured in step 4) is: calculating the concentration of the hormone to be detected by using the calibration value obtained in the step 3) through a piecewise linear approximation algorithm.
Preferably, in the method of the invention, the hormone is luteinizing hormone LH.
Preferably, the hormone concentration measuring device is an ovulation test device.
The invention has the following beneficial effects:
the method for improving the hormone concentration measuring equipment collects the concentration values of a plurality of standard substances, correspondingly prepares a plurality of calibration rods, calibrates the plurality of concentrations, calculates the concentrations by a piecewise linear approximation algorithm, prepares the calibration rods by printing paper slips with specific gray level gradually changed, and keeps the stability of data and the consistency of the standard substances by utilizing the corresponding relation between the collected values and the gray level, so compared with the existing measuring method of the hormone concentration measuring equipment, the measuring accuracy is obviously improved.
Drawings
FIG. 1 is a color selection strip of the method of the present invention.
Fig. 2 is a partial enlarged view at a1 in fig. 1.
Fig. 3 is a partial enlarged view at a2 in fig. 1.
FIG. 4 is a graph of a fitted relationship of absorption amount and concentration of the first hormone concentration measuring device.
Detailed Description
The following further describes the embodiments of the present invention.
The starting materials used in the present invention are commercially available or commonly used in the art, unless otherwise specified, and the methods in the following examples are conventional in the art, unless otherwise specified.
The method is illustrated by taking a product of the company, namely a Femometer IVy (serial number of ASIN: B07YXY2RQB on Amazon electronic commerce platform) as a hormone concentration measuring device to test luteinizing hormone LH as an example, taking LH concentration of 25miu/mL as an LH sample to be tested, and verifying a measurement result;
the collection value mentioned in the invention is the residual quantity of light after being absorbed by the test paper strip or the pure color printing paper strip with the corresponding gray scale.
A method of improving the measurement accuracy of a hormone concentration measurement device, comprising the steps of:
1) detecting a series of standard substances with the concentration of hormone LH to be detected being 0, 5, 10, 20, 35, 45 and 65miu/mL by using a first hormone concentration measuring device, wherein the detection method comprises the steps of reacting the standard substances with a test paper rod, manufacturing the test paper rod by using a test paper strip, measuring the test paper rod by using the first hormone concentration measuring device, measuring each standard substance for 10 times, recording the acquisition value of each time, calculating the average value, the absorption amount and the variance of the acquisition value, and recording the data as the standard data of the corresponding concentration on the first hormone concentration measuring device, wherein the data are shown in Table 1, and the difference between the average value of the acquisition value of a blank sample with the absorption amount of 0 and the average value of the acquisition value under a certain concentration;
2) a stable calibration rod is manufactured by the following preparation method:
(1) preparing a color selecting paper strip: selecting a printing paper strip with 500mm length and 27mm width and gray level gradient as a color selection paper strip; the color selection paper strip is provided with two parallel color bands extending along the length direction of the printing paper strip, wherein the color of one color band is CMYK00047, the color of the other color band is gradually changed from CMYK0007 at one end to CMYK000100 at the other end, and the color selection paper strip is also provided with labels 1 to 119 distributed along the length direction and the like and positioned below the color bands, wherein the label 1 corresponds to one end with the color of CMYK0007, and the label 119 corresponds to one end with the color of CMYK000100, as shown in FIGS. 1 to 3;
the corresponding relation between the labels and the color numbers on the color selection paper strip is as follows: y is str-x (str-end)/(119-1),
wherein y is a color number, x is a reference number, str is a color number str-0 xf4f4f4 corresponding to reference number 1, and end is a color number end-0 x221815 corresponding to reference number 119;
(2) determining the color number: finding the corresponding color number on the color selection paper strip by the minimum dichotomy of the acquired value recorded in the step 1), thereby determining the corresponding label;
(3) preparing a calibration rod: preparing a calibration rod by using the pure-color printing paper strips with the color numbers corresponding to the labels determined in the step (2);
3) the second hormone concentration measuring equipment acquires the gray value of the calibration rod prepared by corresponding different pure-color printing paper strips to obtain the acquired value of the concentration of the standard substance on the second hormone concentration measuring equipment, and calculates the average value, the absorption amount and the variance of the acquired value according to the acquired value, and the result is shown in table 2;
4) on the basis of the above steps, the average value of the collected values of the LH sample (reagent is a standard substance with a concentration of 25 miu/mL) measured by the second hormone concentration measuring device for 10 times is 745.1, and then the measured value of the LH sample on the second hormone concentration measuring device is calculated by using the data recorded in table 2 and using a piecewise linear approximation algorithm, wherein the calculation process is as follows:
two points (20, 168.8) and (35, 197.2) corresponding to the two average values 755 and 726.6 of the collected values on two sides of the average value 745.1 of the collected values are selected from table 2 to make a straight line, and a fitted linear equation is obtained as follows: y-0.528 x-69.1264, wherein y represents concentration and x represents absorption;
the difference 178.69 between the average value 923.8 and the average value 745.1 of the collected values at the concentration of 0 is taken as the absorption amount of the LH sample on the second hormone concentration measuring device;
substitution of 178.6 into the fitting equation y of 0.528x-69.1264 yields y of 25.2219, i.e. a measurement of 25.2219miu/mL of standard substance at a concentration of 25miu/mL on the second hormone concentration measuring device.
The test results were as follows:
TABLE 1 first hormone concentration measurement device log data
TABLE 2 second hormone concentration measurement device log data
Comparison results
The measurement value of the standard substance at a concentration of 25miu/ml on the second hormone concentration measuring device was calculated using the existing coefficient adjustment method c ═ b/a, as follows:
1) fitting a relation between y and x according to the data of the absorption amount and the concentration in table 1 to obtain a fitting curve, as shown in fig. 4, the relation is as follows: y 4.2 x 10-6*x3-0.00081*x2+0.12*x-0.13；
2) Taking a standard substance of 20miu/mL as a calibration sample to obtain an average value of a collection value of 755 on a second hormone concentration measuring device;
3) based on the standard data in table 1 of 20miu/mL, the adjustment coefficient was calculated as c 755/792.8-0.952;
4) calculating the theoretical collection value of the LH sample after adjustment on a second hormone concentration measuring device to be 745.1/0.952-782.668 by using the adjustment coefficient c;
5) the theoretical absorption of the LH sample after adjustment on the second hormone concentration measurement device was calculated as: 974.6-782.668 ═ 191.932;
6) substituting the theoretical absorption amount into the fitted relation in the step 1) to obtain: and y is 22.8308 miu/mL.
The method can obviously improve the measurement accuracy of the hormone concentration measurement equipment by comparing the determination result values of 25.2219miu/mL and 22.8308 miu/mL.
Claims (6)
1. A method of improving the measurement accuracy of a hormone concentration measurement device, comprising the steps of:
1) detecting a series of standard products with different concentrations of hormone to be detected by using first hormone concentration measuring equipment, and recording data, wherein the data comprises the concentration of the standard product, a plurality of times of acquisition values of the standard product with corresponding concentration and an average value of the acquisition values, and the average value is used as standard data of the corresponding concentration on the first hormone concentration measuring equipment;
2) correspondingly preparing stable calibration rods according to the acquisition values of different concentrations and different times in the step 1);
3) the second hormone concentration measuring equipment acquires information of the calibration rod as an acquired value of the corresponding concentration on the second hormone concentration measuring equipment in the step 1), and records data;
4) measuring the collection value of the hormone to be measured by second hormone concentration measuring equipment, and calculating the concentration of the hormone to be measured by the recorded data in the step 3);
the calibration rod is prepared by the following method:
(1) preparing a color selecting paper strip: selecting a printing paper strip with the gray scale gradually changed and the length of 500mm as a color selecting paper strip; the color selection paper strip is provided with two parallel color bands extending along the length direction of the printing paper strip, wherein the color of one color band is CMYK00047, the color of the other color band is gradually changed from CMYK0007 at one end to CMYK000100 at the other end, and the color selection paper strip is provided with labels 1-119 distributed along the length direction, wherein the label 1 corresponds to one end of CMYK0007 in color, and the 119 corresponds to one end of CMYK000100 in color;
the corresponding relation between the labels and the color numbers on the color selection paper strip is as follows: y = str-x (str-end)/(119-1),
wherein y is a color number, x is a reference number, str is a color number str = 0xf4f4f4 corresponding to the reference number 1, and end is a color number end = 0x221815 corresponding to the reference number 119;
(2) determining the color number: finding the corresponding color number on the color selection paper strip according to the acquisition value recorded in the step 1), and determining the corresponding label;
(3) preparing a calibration rod: and (3) preparing the calibration rod by using the pure-color printing paper strips with the marks corresponding to the color numbers determined in the step (2).
2. The method for improving the measurement accuracy of the hormone concentration measurement device of claim 1, wherein the method for detecting the series of standards of different concentrations of the hormone to be measured by the first hormone concentration measurement device comprises the following steps: the standard is reacted with a corresponding test strip rod, and then the test strip rod is measured by a first hormone concentration measuring device.
3. The method for improving the measurement accuracy of the hormone concentration measurement device according to claim 1, wherein the method for collecting the information on the calibration rod by the second hormone concentration measurement device in the step 3) comprises the following steps: and the second hormone concentration measuring equipment acquires the gray value of the calibration rod prepared by the pure-color printing paper strip, obtains the acquired value of the concentration of the standard substance on the second hormone concentration measuring equipment, and records data.
4. The method for improving the measurement accuracy of the hormone concentration measurement device according to claim 1, wherein the method for calculating the hormone concentration to be measured in the step 4) comprises the following steps: calculating the concentration of the hormone to be detected by using the acquired value obtained in the step 3) through a piecewise linear approximation algorithm.
5. The method of improving the measurement accuracy of a hormone concentration measurement device of claim 1 wherein the hormone is luteinizing hormone, LH.
6. The method for improving the measurement accuracy of a hormone concentration measuring device of claim 1 or 5 wherein the hormone concentration measuring device is an ovulation test device.
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| US5545895A (en) * | 1995-03-20 | 1996-08-13 | The Dow Chemical Company | Method of standardizing data obtained through mass spectrometry |
| US20090246075A1 (en) * | 2008-03-25 | 2009-10-01 | Health & Life Co., Ltd. | Biosensing device |
| WO2014025415A2 (en) * | 2012-08-08 | 2014-02-13 | Scanadu Incorporated | Method and apparatus for performing and quantifying color changes induced by specific concentrations of biological analytes in an automatically calibrated environment |
| BR112017016814B1 (en) * | 2015-02-06 | 2021-04-20 | Life Technologies Corporation | method and system for calibrating biological instruments, and non-transitory computer-readable storage media |
| EP3335638A1 (en) * | 2016-12-13 | 2018-06-20 | Colorimetrix GmbH | Method and device for estimation of ovulation date |
| CN109799358A (en) * | 2019-01-29 | 2019-05-24 | 河北艾欧路生物科技有限责任公司 | A kind of preparation method of calibration and its compound calibration of biochemical reagents of biochemistry prover |
| CN110501458A (en) * | 2019-07-29 | 2019-11-26 | 深圳市锦瑞生物科技有限公司 | A kind of concentration measuring instrument calibration method, device and concentration measuring instrument |
| CN111122775A (en) * | 2019-12-10 | 2020-05-08 | 北京蛙鸣华清环保科技有限公司 | Pollution concentration monitoring equipment-oriented segmentation data calibration method and system |
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