CN111811863B - Tissue treatment device for protein purification - Google Patents

Tissue treatment device for protein purification Download PDF

Info

Publication number
CN111811863B
CN111811863B CN202010636182.7A CN202010636182A CN111811863B CN 111811863 B CN111811863 B CN 111811863B CN 202010636182 A CN202010636182 A CN 202010636182A CN 111811863 B CN111811863 B CN 111811863B
Authority
CN
China
Prior art keywords
plate
bevel gear
gear
cabin
tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202010636182.7A
Other languages
Chinese (zh)
Other versions
CN111811863A (en
Inventor
李庆华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hunan Possible Biotechnology Co.,Ltd.
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN202010636182.7A priority Critical patent/CN111811863B/en
Publication of CN111811863A publication Critical patent/CN111811863A/en
Application granted granted Critical
Publication of CN111811863B publication Critical patent/CN111811863B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention relates to the field of protein purification, in particular to a tissue treatment device for protein purification. The invention aims to provide a tissue treatment device for purifying proteins. The tissue treatment device for purifying protein comprises a machine body bottom plate, an isolation sampling mechanism, an internal tissue sampling mechanism, a tissue cell crushing mechanism, an external sealing communication mechanism, an operation control screen and the like; and the left side of the top end of the bottom plate of the machine body is welded with the top cabin plate. According to the invention, aseptic sample input is realized, sealed and isolated sampling is completed after sample sterilization, fresh tissue inside is extracted after the sample is stretched into the tissue of the original sample, so that the cell characteristics are unchanged, the protein is not denatured, the interference of original impurity bacteria on the outer surface of the sample is prevented, fine grinding after mincing is realized, and the device is always in a sealed and isolated state before and after treatment, so that the effect of aseptic cell mixed solution is obtained.

Description

Tissue treatment device for protein purification
Technical Field
The invention relates to the field of protein purification, in particular to a tissue treatment device for protein purification.
Background
The separation and purification of proteins are widely used in biochemical research applications and are an important operation technique. A typical eukaryotic cell may contain thousands of different proteins, some in very rich quantities, and some containing only a few copies. In order to study a protein, it is necessary to first purify the protein from other proteins and non-protein molecules.
In the prior art, most of protein purification is to directly collect cell tissues for subsequent treatment, but most of collected organism samples are samples stored in a laboratory, when manually collecting samples, the next small sample tissue is separated from the outer surface of the sample tissues by means of tweezers, scissors and the like, although the samples can be stored in a sterile laboratory, at the moment of contacting the samples with air, bacterial spores in the air are adhered to the tissue surfaces, and meanwhile, the samples are manually sampled and treated and fully exposed to the air environment, a physical single cell lysis method is often adopted in the tissue cell treatment stage, so that a part of samples obtained from the tissue samples are adhered with a large amount of bacterial fungi, the outer surfaces of the sample tissues are contacted with the air for a long time, although the samples are slightly oxidized and denatured under refrigeration conditions, so that the finally purified proteins are not target proteins, and certain characteristic functions of the proteins are lost.
Accordingly, there is a need to develop a tissue processing device for protein purification to overcome the above problems.
Disclosure of Invention
In order to overcome the defects that most collected biological samples are samples stored in a laboratory, when the samples are manually collected, the next small sample tissue is separated from the outer surface of the sample tissue by means of tweezers, scissors and other tools, although the samples can be stored in a sterile laboratory, bacterial spores in the air are adhered to the tissue surface at the moment of contacting the samples with air, meanwhile, the samples are manually sampled and treated and are completely exposed to the air environment, a physical single cell lysis method is often adopted in the tissue cell treatment stage, so that a large amount of bacterial fungi are adhered to part of samples obtained from the tissue samples, the outer surface of the sample tissue is contacted with the air for a long time, although the surfaces of the sample tissue are in cold storage conditions, slight oxidization and protein denaturation are caused, so that finally purified proteins are not target proteins, certain characteristic functions of the proteins are lost, and meanwhile, a specific organelle in the cells cannot be lysed by the single physical cell lysis method, and part of the cell proteins cannot be extracted.
The invention is achieved by the following specific technical means:
the tissue treatment device for purifying the protein comprises a bottom plate of a machine body, a top cabin plate, a first connecting cabin plate, a second connecting cabin plate, a third connecting cabin plate, an isolation sampling mechanism, an internal tissue sampling mechanism, a tissue cell crushing mechanism, an external sealing communication mechanism and an operation control screen; the left side of the top end of the bottom plate of the machine body is welded with the top cabin plate; the right side of the top end of the bottom plate of the machine body is welded with the first connecting cabin plate; the left side of the top end of the machine body bottom plate is connected with the isolation sampling mechanism, and the left middle part of the isolation sampling mechanism is connected with the top cabin plate; the right side of the top end of the machine body bottom plate is connected with the tissue cell disruption mechanism, the right lower part of the tissue cell disruption mechanism is connected with the first connecting cabin plate, and the left side of the tissue cell disruption mechanism is connected with the isolation sampling mechanism; the middle part of the inner top end of the top cabin plate is connected with an internal tissue sampling mechanism, the bottom of the left end of the internal tissue sampling mechanism is connected with an isolation sampling mechanism, and the right lower part of the internal tissue sampling mechanism is connected with a tissue cell crushing mechanism; the right side of the inner top end of the top cabin plate is welded with a third connecting cabin plate, and the middle lower part of the left end of the third connecting cabin plate is connected with an internal tissue sampling mechanism; the top of the left end of the first connecting cabin plate is welded with the second connecting cabin plate, the left part of the second connecting cabin plate is connected with the tissue cell crushing mechanism, and the middle part of the top end of the second connecting cabin plate is connected with the third connecting cabin plate; the middle upper part of the right end of the third connecting cabin plate is sleeved with an external sealing communication mechanism; and an operation control screen is arranged at the lower side of the right end of the third connecting cabin board.
The isolation sampling mechanism comprises a power motor, a first driving wheel, a first spring rod, a belt control wheel, a second driving wheel, a first driving gear, a first electric push rod, a toothed bar, a limit sliding rail, a buffer limiting plate, a buffer spring, a damping slip ring, a first electric sliding rail, a first electric sliding block, a sample sampling vessel, a partition plate, a top clamping plate, a first dead angle-free ultraviolet emitter and a first frame plate; the middle part of the front end of the power motor is rotationally connected with the first driving wheel; a first spring rod is arranged at the upper left part of the power motor; the right upper part of the first driving wheel is in driving connection with the second driving wheel; the right end of the first spring rod is rotationally connected with the belt control wheel through a bearing; the middle part of the front end of the second driving wheel is rotationally connected with the first driving gear; the middle part of the rear end of the second driving wheel is rotationally connected with the first electric push rod through a bearing; the middle part of the right end of the first transmission gear is meshed with the toothed bar; the lower side of the right end of the toothed bar is in sliding connection with the limit sliding rail; the top end of the toothed bar is spliced with the isolation plate; the middle lower part of the left end of the limit sliding rail is in sliding connection with the buffer limit plate; the bottom end of the buffer limiting plate is connected with a buffer spring; the bottom of the outer surface of the isolation plate is in sliding connection with the damping slip ring; the top end of the damping slip ring is connected with the first electric sliding rail through bolts; the left side of the top end of the first electric sliding rail is in sliding connection with the first electric sliding block; the right end of the first electric sliding rail is connected with the first frame plate; the top end of the first electric sliding block is sleeved with the sample sampling vessel; the top end of the isolation plate is spliced with the top clamping plate; the right side of the bottom end of the top clamping plate is provided with a first dead-angle-free ultraviolet emitter; the bottom end of the first frame plate is connected with the bottom plate of the machine body; the left end of the top clamping plate is connected with the top cabin plate; the right end of the top clamping plate is connected with an internal tissue sampling mechanism; the left end of the first electric sliding rail is connected with the top cabin plate; the left end of the first electric push rod is connected with the top cabin plate; the left end of the first spring rod is connected with the top cabin plate; the bottom end of the power motor is connected with the top cabin plate; the bottom end of the buffer spring is connected with the bottom plate of the machine body; the bottom end of the limit sliding rail is connected with the bottom plate of the machine body; the upper right side of the first driving wheel is connected with a tissue cell crushing mechanism.
The internal tissue sampling mechanism comprises a third driving wheel, a first gear plate, a first flat gear, a drawing mechanism, a second electric sliding rail, a second electric sliding block, a third electric sliding rail, a fourth driving wheel, a first bevel gear, a second flat gear, a third flat gear and a fourth flat gear; the middle part of the top end of the third driving wheel is rotationally connected with the first gear plate through a bearing; the middle part of the bottom end of the third driving wheel is rotationally connected with the first flat gear; the right side of the third driving wheel is in driving connection with the fourth driving wheel, and the top end of the outer surface of the fourth driving wheel is connected with the first gear plate; the middle part of the right end of the first flat gear is meshed with the withdrawing mechanism; the bottom of the rear end of the extraction mechanism is in sliding connection with the second electric sliding rail; the left end of the second electric sliding rail is connected with the second electric sliding block through a bolt; the rear end of the second electric sliding block is in sliding connection with a third electric sliding rail; the middle part of the top end of the fourth driving wheel is rotationally connected with the first bevel gear; the middle part of the rear end of the first bevel gear is meshed with the second bevel gear, and the middle part of the rear end of the second bevel gear is connected with the first gear plate; the middle part of the right end of the second flat gear is meshed with the third flat gear, and the middle part of the top end of the third flat gear is connected with the first gear plate; the middle part of the bottom end of the third flat gear is rotationally connected with the fourth flat gear; the top end of the first gear plate is connected with the top cabin plate; the right lower part of the second bevel gear is connected with the tissue cell disruption mechanism; the right end of the second electric sliding rail is connected with a third connecting cabin plate; the middle part of the left end of the third electric sliding rail is connected with the top clamping plate.
The tissue cell crushing mechanism comprises a blanking pipe, a steering spray head, a spray head frame, a flushing flow channel plate, a crushing cabin, a first bearing strip, a first rotating shaft rod, a crushing cutter, a second bearing plate, a third bevel gear, a fourth bevel gear, a fifth bevel gear, a sixth bevel gear, a second rotating shaft rod, a connecting inclined rod, a grinding ball, a special-shaped mortar and a sampling valve; the bottom end of the blanking pipe is spliced with the steering spray head; the middle part of the outer surface of the steering spray head is sleeved with the spray head frame; the bottom of the right end of the spray head frame is connected with a flushing flow channel plate; a crushing cabin is arranged at the right lower part of the flushing flow channel plate; the middle part in the crushing cabin is provided with a first bearing strip; the middle part of the first bearing strip is spliced with the first rotating shaft rod; the middle part of the bottom end of the first rotating shaft rod is rotationally connected with the crushing cutter; the top end of the outer surface of the first rotating shaft rod is spliced with the second bearing plate; the top end of the first rotating shaft rod is rotationally connected with the third bevel gear; the middle part of the rear end of the third bevel gear is meshed with a fourth bevel gear, and the middle part of the rear end of the fourth bevel gear is connected with a second bearing plate; the right lower part of the fourth bevel gear is in transmission connection with the fifth bevel gear; the middle part of the bottom end of the fifth bevel gear is meshed with the sixth bevel gear; the middle part of the bottom end of the sixth bevel gear is rotationally connected with the second rotating shaft rod; the bottom end of the second rotating shaft rod is spliced with the connecting diagonal rod; the bottom end of the connecting diagonal rod is spliced with the grinding ball; a special-shaped mortar is arranged below the grinding ball; the right side of the special-shaped mortar is provided with a sampling valve; the outer surface of the sampling valve is connected with the first connecting cabin plate; the bottom end of the special-shaped mortar is connected with the bottom plate of the machine body; the bottom end of the crushing cabin is connected with the machine body bottom plate; the middle part of the bottom end of the flushing flow channel plate is connected with the bottom plate of the machine body; the top end of the outer surface of the blanking pipe is connected with the top cabin plate; the right end of the second bearing plate is connected with the second connecting cabin plate; the lower side of the outer surface of the second rotating shaft rod is connected with a second connecting cabin plate; the left upper part of the fourth bevel gear is connected with the second bevel gear; the left lower part of the fourth bevel gear is connected with a first driving wheel; the middle part of the rear end of the fifth bevel gear is connected with the second connecting cabin plate.
The external sealing communication mechanism comprises a screwed sealing cover, an outer cylinder, a second dead-angle-free ultraviolet emitter, an embedded cylinder and a latex sleeve; screwing the lower side of the outer surface of the sealing cover to be screwed with the connecting outer cylinder; a plurality of groups of second dead-angle-free ultraviolet emitters are equidistantly arranged on the circumference of the outer ring at the top end of the connecting outer cylinder; the bottom end of the connecting outer cylinder is sleeved with the embedded cylinder; the middle part of the bottom end of the embedded cylinder is bonded with the latex sleeves; the outer surface of the embedded cylinder is connected with a third connecting cabin plate.
The extraction mechanism comprises a third electric sliding block, an outer ring plate, a sampling tube, a sampling large-caliber needle head, an extraction piston, a piston connecting rod, an internal thread slip ring, a screw rod and a fifth flat gear; the top of the front end of the third electric sliding block is spliced with the connecting outer ring plate; the bottom end of the outer ring plate is connected with the sampling tube for welding; the middle part of the bottom end of the sampling tube is spliced with a sampling large-caliber needle head; the inner surface of the sampling tube is in sliding connection with the drawing and discharging piston; the middle part of the top end of the drawing-out piston is spliced with the piston connecting rod; the top of the rear end of the piston connecting rod is connected with the internal thread slip ring through bolts; the inner surface of the internal thread sliding ring is rotationally connected with the screw rod, and the top end and the bottom end of the outer surface of the screw rod are connected with a third electric sliding block; the middle part of the top end of the screw rod is rotationally connected with a fifth flat gear; the middle part of the left end of the fifth flat gear is connected with the first flat gear; the middle part of the rear end of the third electric sliding block is connected with a second electric sliding rail.
The three groups of external sealing communication mechanisms are respectively positioned in the middle of the third connecting cabin plate, the left side of the top cabin plate and the middle of the left end of the top cabin plate, and the same external sealing communication mechanisms positioned in the middle of the left end of the top cabin plate are not provided with latex sleeves.
The groove at the top end of the special-shaped mortar is arranged in an annular shape which keeps fit with the grinding ball at all times when the grinding ball rotates.
The right end of the second electric sliding rail is provided with a strip-shaped auxiliary damping sliding rail.
The length of the first flat gear is twice the length of the fifth flat gear.
Compared with the prior art, the invention has the following beneficial effects:
in order to solve the problems that most of collected biological samples in the prior art are samples stored in a laboratory, when the samples are manually collected, the next small sample tissue is separated from the outer surface of the sample tissue by means of tweezers, scissors and other tools, although the samples can be stored in a sterile laboratory, bacterial spores in the air are adhered to the tissue surface at the moment of contacting the samples with air, meanwhile, the samples are manually sampled and treated and are completely exposed in an air environment, a physical single cell lysis method is often adopted in a tissue cell treatment stage, so that a large amount of bacterial fungi are adhered to part of samples obtained from the tissue samples, the outer surface of the sample tissue is contacted with the air for a long time, slight oxidization and protein denaturation are caused although the sample tissue is in a refrigerating condition, so that finally purified proteins are not target proteins, certain characteristic functions of the proteins are lost, and meanwhile, the specific organelles in the cells cannot be lysed by the single physical cell lysis method, and further part of the cell proteins cannot be extracted;
The method comprises the steps of designing an isolation sampling mechanism, an internal tissue sampling mechanism and a tissue cell crushing mechanism, firstly carrying out ultraviolet sterilization on a tissue sample through the isolation sampling mechanism, carrying out isolation transmission, then extracting a fresh sample in the tissue sample through the internal tissue sampling mechanism, and then transmitting the fresh tissue sample into the tissue cell crushing mechanism through the internal tissue sampling mechanism, namely mincing and grinding fresh sample tissues;
the aseptic sample input is realized, the sample is sealed and isolated after the sample is sterilized, and the sample directly stretches into the tissue of the sample to extract the fresh tissue inside, so that the cell characteristics are unchanged, the protein is intact, the protein has no denaturation and complete functions, the interference of original impurity bacteria on the outer surface of the sample is prevented, the fine grinding after the grinding is realized, the cell mixed solution with higher crushing degree is obtained, and the device is always in a sealed and isolated state before and after the treatment, so that the effect of the aseptic cell mixed solution is obtained.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of an isolated sampling mechanism according to the present invention;
FIG. 3 is a schematic diagram of the internal tissue sampling mechanism of the present invention;
FIG. 4 is a schematic diagram of a tissue cell disruption mechanism according to the present invention;
FIG. 5 is a schematic view of the external sealing communication mechanism of the present invention;
FIG. 6 is a schematic structural view of a pulling-out mechanism according to the present invention;
fig. 7 is a top view of the shaped mortar of the invention.
The marks in the drawings are: 1-machine floor, 2-top deck, 3-first connection deck, 4-second connection deck, 5-third connection deck, 6-isolation sampling mechanism, 7-internal tissue sampling mechanism, 8-tissue cell disruption mechanism, 9-external sealed communication mechanism, 10-run control screen, 601-power motor, 602-first drive wheel, 603-first spring bar, 604-belt control wheel, 605-second drive wheel, 606-first drive gear, 607-first electric push rod, 608-toothed bar, 609-limit slide rail, 6010-buffer stop plate, 6011-buffer spring, 6012-damping slide ring, 6013-first electric slide rail, 6014-first electric slide block, 6015-sample sampling dish, 6016-isolation plate, 6017-top card, 6018-first dead angle free ultraviolet transmitter, 6019-first frame plate, 701-third drive wheel, 702-first toothed bar, 703-first flat gear, 704-drawer mechanism, 705-second electric slide rail, 706-third electric slide rail, 709-third electric slide rail, 70803-fourth electric slide rail, 70803-second flat gear, 70803-second rotary table, 70805, 70803-fourth agitating brush head pipe, 7013-second rotary table, 70803-second electric slide rail, 70803-third agitating brush head pipe, 70803-spiral jet pipe, 7013-agitating nozzle, spray nozzle, and spray nozzle pipe, 8010-third bevel gear, 8011-fourth bevel gear, 8012-fifth bevel gear, 8013-sixth bevel gear, 8014-second rotating shaft rod, 8015-connecting bevel rod, 8016-grinding ball, 8017-special-shaped mortar, 8018-sampling valve, 901-screwing sealing cover, 902-connecting outer cylinder, 903-second dead-angle-free ultraviolet emitter, 904-embedding cylinder, 905-latex sleeve, 70401-third electric sliding block, 70402-connecting outer ring plate, 70403-sampling cylinder, 70404-sampling large-caliber needle, 70405-drawing piston, 70406-piston connecting rod, 70407-internal thread slip ring, 70408-screw rod and 70409-fifth flat gear.
Detailed Description
The invention is further described below with reference to the drawings and examples.
Examples
1-7, the tissue treatment device for protein purification comprises a machine body bottom plate 1, a top cabin plate 2, a first connecting cabin plate 3, a second connecting cabin plate 4, a third connecting cabin plate 5, an isolation sampling mechanism 6, an internal tissue sampling mechanism 7, a tissue cell crushing mechanism 8, an external sealing communication mechanism 9 and an operation control screen 10; the left side of the top end of the machine body bottom plate 1 is welded with the top cabin plate 2; the right side of the top end of the machine body bottom plate 1 is welded with the first connecting cabin plate 3; the left side of the top end of the machine body bottom plate 1 is connected with an isolation sampling mechanism 6, and the left middle part of the isolation sampling mechanism 6 is connected with a top cabin plate 2; the right side of the top end of the machine body bottom plate 1 is connected with a tissue cell disruption mechanism 8, the right lower part of the tissue cell disruption mechanism 8 is connected with a first connecting cabin plate 3, and the left side of the tissue cell disruption mechanism 8 is connected with an isolation sampling mechanism 6; the middle part of the inner top end of the top cabin plate 2 is connected with an internal tissue sampling mechanism 7, the bottom of the left end of the internal tissue sampling mechanism 7 is connected with an isolation sampling mechanism 6, and the right lower part of the internal tissue sampling mechanism 7 is connected with a tissue cell crushing mechanism 8; the right side of the inner top end of the top cabin plate 2 is welded with a third connecting cabin plate 5, and the middle lower part of the left end of the third connecting cabin plate 5 is connected with an internal tissue sampling mechanism 7; the top of the left end of the first connecting cabin plate 3 is welded with the second connecting cabin plate 4, the left part of the second connecting cabin plate 4 is connected with the tissue cell crushing mechanism 8, and the middle part of the top end of the second connecting cabin plate 4 is connected with the third connecting cabin plate 5; the middle upper part of the right end of the third connecting cabin plate 5 is sleeved with an external sealing communication mechanism 9; the lower side of the right end of the third connecting deck plate 5 is provided with an operation control screen 10.
The isolation sampling mechanism 6 comprises a power motor 601, a first driving wheel 602, a first spring rod 603, a belt control wheel 604, a second driving wheel 605, a first driving gear 606, a first electric push rod 607, a toothed bar 608, a limit sliding rail 609, a buffer limiting plate 6010, a buffer spring 6011, a buffer sliding ring 6012, a first electric sliding rail 6013, a first electric sliding block 6014, a sample sampling vessel 6015, an isolation plate 6016, a top clamping plate 6017, a first dead angle-free ultraviolet emitter 6018 and a first frame plate 6019; the middle part of the front end of the power motor 601 is rotationally connected with a first driving wheel 602; a first spring rod 603 is arranged at the upper left of the power motor 601; the right upper part of the first driving wheel 602 is in driving connection with a second driving wheel 605; the right end of the first spring rod 603 is rotationally connected with a belt control wheel 604 through a bearing; the middle part of the front end of the second driving wheel 605 is rotationally connected with a first driving gear 606; the middle part of the rear end of the second driving wheel 605 is rotationally connected with a first electric push rod 607 through a bearing; the middle part of the right end of the first transmission gear 606 is meshed with a toothed bar 608; the lower side of the right end of the toothed bar 608 is in sliding connection with a limit sliding rail 609; the top end of the toothed bar 608 is spliced with the partition plate 6016; the middle lower part of the left end of the limit sliding rail 609 is in sliding connection with the buffer limit plate 6010; the bottom end of the buffer limiting plate 6010 is connected with a buffer spring 6011; the bottom of the outer surface of the isolation plate 6016 is in sliding connection with the damping slip ring 6012; the top end of the damping slip ring 6012 is connected with a first electric sliding rail 6013 through bolts; the left side of the top end of the first electric sliding rail 6013 is in sliding connection with the first electric sliding block 6014; the right end of the first electric sliding rail 6013 is connected with the first frame plate 6019; the top end of the first electric sliding block 6014 is sleeved with the sample sampling vessel 6015; the top end of the partition plate 6016 is spliced with the top clamping plate 6017; the right side of the bottom end of the top clamping plate 6017 is provided with a first dead-angle-free ultraviolet emitter 6018; the bottom end of the first frame plate 6019 is connected with the machine body bottom plate 1; the left end of the top clamping plate 6017 is connected with the top cabin plate 2; the right end of the top clamping plate 6017 is connected with an internal tissue sampling mechanism 7; the left end of the first electric sliding rail 6013 is connected with the top cabin board 2; the left end of the first electric push rod 607 is connected with the top cabin board 2; the left end of the first spring rod 603 is connected with the top deck plate 2; the bottom end of the power motor 601 is connected with the top deck plate 2; the bottom end of the buffer spring 6011 is connected with the machine body bottom plate 1; the bottom end of the limit sliding rail 609 is connected with the machine body bottom plate 1; the upper right side of the first driving wheel 602 is connected with the tissue cell disruption mechanism 8.
The internal tissue sampling mechanism 7 includes a third transmission wheel 701, a first gear plate 702, a first flat gear 703, a withdrawing mechanism 704, a second electric slide 705, a second electric slide 706, a third electric slide 707, a fourth transmission wheel 708, a first bevel gear 709, a second bevel gear 7010, a second flat gear 7011, a third flat gear 7012, and a fourth flat gear 7013; the middle part of the top end of the third driving wheel 701 is rotationally connected with the first gear plate 702 through a bearing; the middle part of the bottom end of the third driving wheel 701 is rotationally connected with a first flat gear 703; the right side of the third driving wheel 701 is in driving connection with the fourth driving wheel 708, and the top end of the outer surface of the fourth driving wheel 708 is connected with the first gear plate 702; the middle part of the right end of the first flat gear 703 is meshed with the withdrawing mechanism 704; the bottom of the rear end of the withdrawing mechanism 704 is in sliding connection with a second electric sliding rail 705; the left end of the second electric sliding rail 705 is connected with a second electric sliding block 706 through a bolt; the rear end of the second electric sliding block 706 is in sliding connection with a third electric sliding rail 707; the middle part of the top end of the fourth driving wheel 708 is rotationally connected with a first bevel gear 709; the middle part of the rear end of the first bevel gear 709 is meshed with the second bevel gear 7010, and the middle part of the rear end of the second bevel gear 7010 is connected with the first gear plate 702; the middle part of the right end of the second flat gear 7011 is meshed with the third flat gear 7012, and the middle part of the top end of the third flat gear 7012 is connected with the first gear plate 702; the middle part of the bottom end of the third flat gear 7012 is rotationally connected with a fourth flat gear 7013; the top end of the first gear plate 702 is connected with the top deck 2; the right lower part of the second bevel gear 7010 is connected with a tissue cell disruption mechanism 8; the right end of the second electric sliding rail 705 is connected with a third connecting cabin board 5; the middle of the left end of the third power rail 707 is connected to the top card 6017.
The histiocyte crushing mechanism 8 comprises a blanking pipe 801, a steering nozzle 802, a nozzle frame 803, a flushing flow channel plate 804, a crushing cabin 805, a first bearing bar 806, a first rotating shaft rod 807, a crushing cutter 808, a second bearing plate 809, a third bevel gear 8010, a fourth bevel gear 8011, a fifth bevel gear 8012, a sixth bevel gear 8013, a second rotating shaft rod 8014, a connecting diagonal rod 8015, a grinding ball 8016, a special-shaped mortar 8017 and a sampling valve 8018; the bottom end of the blanking pipe 801 is spliced with a steering nozzle 802; the middle part of the outer surface of the steering nozzle 802 is sleeved with a nozzle frame 803; the bottom of the right end of the nozzle frame 803 is connected with a flushing flow channel plate 804; a crushing cabin 805 is arranged at the right lower part of the flushing flow channel plate 804; a first bearing strip 806 is arranged in the middle of the crushing cabin 805; the middle part of the first bearing bar 806 is spliced with the first rotating shaft rod 807; the middle part of the bottom end of the first rotating shaft rod 807 is rotationally connected with a crushing cutter 808; the top end of the outer surface of the first rotating shaft rod 807 is spliced with the second bearing plate 809; the top end of the first rotating shaft rod 807 is rotatably connected with a third bevel gear 8010; the middle part of the rear end of the third bevel gear 8010 is meshed with the fourth bevel gear 8011, and the middle part of the rear end of the fourth bevel gear 8011 is connected with the second bearing plate 809; the right lower part of the fourth bevel gear 8011 is in transmission connection with the fifth bevel gear 8012; the middle part of the bottom end of the fifth bevel gear 8012 is meshed with the sixth bevel gear 8013; the middle part of the bottom end of the sixth bevel gear 8013 is rotationally connected with the second rotating shaft rod 8014; the bottom end of the second rotating shaft rod 8014 is spliced with the connecting inclined rod 8015; the bottom end of the connecting inclined rod 8015 is spliced with the grinding ball 8016; a special-shaped mortar 8017 is arranged below the grinding ball 8016; the right side of the special-shaped mortar 8017 is provided with a sampling valve 8018; the outer surface of the sampling valve 8018 is connected with the first connecting cabin plate 3; the bottom end of the special-shaped mortar 8017 is connected with the machine body bottom plate 1; the bottom end of the crushing cabin 805 is connected with the machine body bottom plate 1; the middle part of the bottom end of the flushing flow channel plate 804 is connected with the machine body bottom plate 1; the top end of the outer surface of the blanking pipe 801 is connected with the top cabin board 2; the right end of the second bearing plate 809 is connected with the second connecting cabin plate 4; the lower side of the outer surface of the second rotating shaft 8014 is connected with the second connecting deck 4; the left upper part of the fourth bevel gear 8011 is connected with the second bevel gear 7010; the left lower part of the fourth bevel gear 8011 is connected with the first driving wheel 602; the middle part of the rear end of the fifth bevel gear 8012 is connected with the second connection deck 4.
The external sealing communication mechanism 9 comprises a screwing sealing cover 901, an outer cylinder 902, a second dead-angle-free ultraviolet emitter 903, an embedded cylinder 904 and a latex sleeve 905; the lower side of the outer surface of the screwing sealing cover 901 is screwed with the connecting outer cylinder 902; a plurality of groups of second dead-angle-free ultraviolet emitters 903 are equidistantly arranged on the circumference of the outer ring at the top end of the connecting outer cylinder 902; the bottom end of the connecting outer cylinder 902 is sleeved with the embedded cylinder 904; the middle part of the bottom end of the embedded cylinder 904 is bonded with a latex sleeve 905; the outer surface of the embedded cylinder 904 is connected with the third connection deck 5.
The withdrawing mechanism 704 comprises a third electric sliding block 70401, an outer connecting ring plate 70402, a sampling tube 70403, a sampling large-caliber needle 70404, a withdrawing piston 70405, a piston connecting rod 70406, an internal threaded slip ring 70407, a screw rod 70408 and a fifth flat gear 70409; the top of the front end of the third electric sliding block 70401 is spliced with a connecting outer ring plate 70402; the bottom end of the outer ring plate 70402 is connected with a sampling tube 70403 for welding; the middle part of the bottom end of the sampling tube 70403 is spliced with a sampling large-caliber needle 70404; the inner surface of the sampling tube 70403 is in sliding connection with the drawing-out piston 70405; the middle part of the top end of the drawing piston 70405 is spliced with a piston connecting rod 70406; the top of the rear end of the piston connecting rod 70406 is connected with an internal thread slip ring 70407 through bolts; the inner surface of the internally threaded slip ring 70407 is rotationally connected with the screw rod 70408, and the top end and the bottom end of the outer surface of the screw rod 70408 are connected with the third electric sliding block 70401; the middle part of the top end of the screw rod 70408 is rotationally connected with a fifth flat gear 70409; the middle part of the left end of the fifth flat gear 70409 is connected with the first flat gear 703; the middle part of the rear end of the third electric sliding block 70401 is connected with a second electric sliding rail 705.
The external sealing communication mechanisms 9 are provided with three groups, which are respectively positioned in the middle of the third connecting deck plate 5, the left side in the top end of the top deck plate 2 and the middle of the left end of the top deck plate 2, and the same external sealing communication mechanism 9 positioned in the middle of the left end of the top deck plate 2 is not provided with a latex sleeve 905.
The groove at the top of the shaped mortar 8017 is formed in a ring shape which is kept in contact with the grinding ball 8016 when it rotates.
The right end of the second electric sliding rail 705 is provided with a bar-shaped auxiliary damping sliding rail.
The length of the first flat gear 703 is twice the length of the fifth flat gear 70409.
Working principle: when the device is used, firstly, the device is transported to a laboratory, then firstly, an animal tissue sample to be subjected to protein extraction and purification by a worker is added into the isolation sampling mechanism 6 through a mechanism which is the same as the external sealing communicating mechanism 9 in the middle of the left end of the top cabin plate 2, then, the device is resealed, then, an external power supply is connected, the operation control screen 10 is manually opened, then, the internal power system of the device is connected through the operation control screen 10, firstly, the isolation sampling mechanism 6 performs ultraviolet sterilization and isolation transmission on the tissue sample, then, the fresh sample in the tissue sample is extracted through the internal tissue sampling mechanism 7, then, the fresh tissue sample is transported into the tissue cell crushing mechanism 8 through the internal tissue sampling mechanism 7, namely, the fresh sample tissue is ground, so that aseptic sample input is realized, sealed isolation sampling is completed after sample disinfection is completed, and the fresh tissue sample is directly stretched into the original sample tissue, so that the cell characteristics are unchanged, the protein is completely undenatured, the interference of the original impurity bacteria on the outer surface of the sample is prevented, fine grinding is realized, the cell mixed liquid with higher crushing degree is obtained, and the cell mixed liquid is always in a sealed state after the treatment and the device is in a sterile and sealed state.
The isolation sampling mechanism 6 comprises a power motor 601, a first driving wheel 602, a first spring rod 603, a belt control wheel 604, a second driving wheel 605, a first driving gear 606, a first electric push rod 607, a toothed bar 608, a limit sliding rail 609, a buffer limiting plate 6010, a buffer spring 6011, a buffer sliding ring 6012, a first electric sliding rail 6013, a first electric sliding block 6014, a sample sampling vessel 6015, an isolation plate 6016, a top clamping plate 6017, a first dead angle-free ultraviolet emitter 6018 and a first frame plate 6019; the middle part of the front end of the power motor 601 is rotationally connected with a first driving wheel 602; a first spring rod 603 is arranged at the upper left of the power motor 601; the right upper part of the first driving wheel 602 is in driving connection with a second driving wheel 605; the right end of the first spring rod 603 is rotationally connected with a belt control wheel 604 through a bearing; the middle part of the front end of the second driving wheel 605 is rotationally connected with a first driving gear 606; the middle part of the rear end of the second driving wheel 605 is rotationally connected with a first electric push rod 607 through a bearing; the middle part of the right end of the first transmission gear 606 is meshed with a toothed bar 608; the lower side of the right end of the toothed bar 608 is in sliding connection with a limit sliding rail 609; the top end of the toothed bar 608 is spliced with the partition plate 6016; the middle lower part of the left end of the limit sliding rail 609 is in sliding connection with the buffer limit plate 6010; the bottom end of the buffer limiting plate 6010 is connected with a buffer spring 6011; the bottom of the outer surface of the isolation plate 6016 is in sliding connection with the damping slip ring 6012; the top end of the damping slip ring 6012 is connected with a first electric sliding rail 6013 through bolts; the left side of the top end of the first electric sliding rail 6013 is in sliding connection with the first electric sliding block 6014; the right end of the first electric sliding rail 6013 is connected with the first frame plate 6019; the top end of the first electric sliding block 6014 is sleeved with the sample sampling vessel 6015; the top end of the partition plate 6016 is spliced with the top clamping plate 6017; the right side of the bottom end of the top clamping plate 6017 is provided with a first dead-angle-free ultraviolet emitter 6018; the bottom end of the first frame plate 6019 is connected with the machine body bottom plate 1; the left end of the top clamping plate 6017 is connected with the top cabin plate 2; the right end of the top clamping plate 6017 is connected with an internal tissue sampling mechanism 7; the left end of the first electric sliding rail 6013 is connected with the top cabin board 2; the left end of the first electric push rod 607 is connected with the top cabin board 2; the left end of the first spring rod 603 is connected with the top deck plate 2; the bottom end of the power motor 601 is connected with the top deck plate 2; the bottom end of the buffer spring 6011 is connected with the machine body bottom plate 1; the bottom end of the limit sliding rail 609 is connected with the machine body bottom plate 1; the upper right side of the first driving wheel 602 is connected with the tissue cell disruption mechanism 8.
Firstly, the same mechanism as an external sealing communicating mechanism 9 is added to the top end of a sample sampling vessel 6015 in an isolation sampling mechanism 6 by opening the middle part of the left end of a top cabin plate 2, then a power motor 601 is connected through a running control screen 10, then the power motor 601 drives a first driving wheel 602 to rotate, then the first driving wheel 602 drives a second driving wheel 605 to rotate, the front end of the second driving wheel 605 drives a first driving gear 606 to rotate, then the first driving gear 606 rotates to drive a toothed bar 608 to move downwards, then the toothed bar 608 drives an isolation plate 6016 to slide downwards on the inner side of a damping sliding ring 6012, the top end of the isolation plate 6016 moves to the lower side of a first electric sliding rail 6013, at the moment, the toothed bar 608 slides downwards to be in contact with the buffer limiting plate 6010, then the buffer spring 6011 to compress and buffer, then the first electric driving rod 607 contracts leftwards by running the control screen 10, then the first electric driving the first driving wheel 606 to move leftwards, then the right end of the first driving gear 606 to be disengaged from the toothed bar 608, then the toothed bar 608 stops moving, then the first electric sliding rail 6013 drives the first electric sliding rail 6016 to move with the first electric sliding rail 6018 to move rightwards, and then the sample is emitted to the dead angle of the sample vessel 6018, and the sample is subjected to a dead angle of ultraviolet radiation sterilizing vessel is transmitted to the sample is in the side of the ultraviolet radiation sterilizing vessel, and the sample is in the ultraviolet radiation sterilizing vessel.
The internal tissue sampling mechanism 7 includes a third transmission wheel 701, a first gear plate 702, a first flat gear 703, a withdrawing mechanism 704, a second electric slide 705, a second electric slide 706, a third electric slide 707, a fourth transmission wheel 708, a first bevel gear 709, a second bevel gear 7010, a second flat gear 7011, a third flat gear 7012, and a fourth flat gear 7013; the middle part of the top end of the third driving wheel 701 is rotationally connected with the first gear plate 702 through a bearing; the middle part of the bottom end of the third driving wheel 701 is rotationally connected with a first flat gear 703; the right side of the third driving wheel 701 is in driving connection with the fourth driving wheel 708, and the top end of the outer surface of the fourth driving wheel 708 is connected with the first gear plate 702; the middle part of the right end of the first flat gear 703 is meshed with the withdrawing mechanism 704; the bottom of the rear end of the withdrawing mechanism 704 is in sliding connection with a second electric sliding rail 705; the left end of the second electric sliding rail 705 is connected with a second electric sliding block 706 through a bolt; the rear end of the second electric sliding block 706 is in sliding connection with a third electric sliding rail 707; the middle part of the top end of the fourth driving wheel 708 is rotationally connected with a first bevel gear 709; the middle part of the rear end of the first bevel gear 709 is meshed with the second bevel gear 7010, and the middle part of the rear end of the second bevel gear 7010 is connected with the first gear plate 702; the middle part of the right end of the second flat gear 7011 is meshed with the third flat gear 7012, and the middle part of the top end of the third flat gear 7012 is connected with the first gear plate 702; the middle part of the bottom end of the third flat gear 7012 is rotationally connected with a fourth flat gear 7013; the top end of the first gear plate 702 is connected with the top deck 2; the right lower part of the second bevel gear 7010 is connected with a tissue cell disruption mechanism 8; the right end of the second electric sliding rail 705 is connected with a third connecting cabin board 5; the middle of the left end of the third power rail 707 is connected to the top card 6017.
Firstly, a sample sampling vessel 6015 drives a sample to move to the lower part of a withdrawing mechanism 704, then the upper left part of a fourth bevel gear 8011 drives a second bevel gear 7010 to rotate, then the second bevel gear 7010 drives a first bevel gear 709 to rotate, further the first bevel gear 709 drives a fourth driving wheel 708 to rotate, then the fourth driving wheel 708 drives a second flat gear 7011 and a third driving wheel 701 to rotate, then the third driving wheel 701 drives a first flat gear 703 to drive a withdrawing mechanism 704 to operate, further a third electric sliding rail 707 drives the second electric sliding rail 705 to communicate with the withdrawing mechanism 704 to move downwards through a second electric sliding block 706, further the withdrawing mechanism 704 stretches into the tissue sample to extract an internal fresh sample, then the withdrawing mechanism 704 moves upwards to a home position again, further the second electric sliding rail 705 drives the withdrawing mechanism 704 to move rightwards to a position meshed with the fourth flat gear 7013, further the second flat gear 7011 drives the fourth flat gear 7013 to rotate, and then the fourth flat gear 7013 controls the fourth flat gear 7013 to drive the withdrawing mechanism 704 to drive the withdrawing mechanism to move downwards to communicate with the withdrawing mechanism 704 to extract the tissue sample to the internal fresh sample 8, and thus the tissue sample is extracted.
The histiocyte crushing mechanism 8 comprises a blanking pipe 801, a steering nozzle 802, a nozzle frame 803, a flushing flow channel plate 804, a crushing cabin 805, a first bearing bar 806, a first rotating shaft rod 807, a crushing cutter 808, a second bearing plate 809, a third bevel gear 8010, a fourth bevel gear 8011, a fifth bevel gear 8012, a sixth bevel gear 8013, a second rotating shaft rod 8014, a connecting diagonal rod 8015, a grinding ball 8016, a special-shaped mortar 8017 and a sampling valve 8018; the bottom end of the blanking pipe 801 is spliced with a steering nozzle 802; the middle part of the outer surface of the steering nozzle 802 is sleeved with a nozzle frame 803; the bottom of the right end of the nozzle frame 803 is connected with a flushing flow channel plate 804; a crushing cabin 805 is arranged at the right lower part of the flushing flow channel plate 804; a first bearing strip 806 is arranged in the middle of the crushing cabin 805; the middle part of the first bearing bar 806 is spliced with the first rotating shaft rod 807; the middle part of the bottom end of the first rotating shaft rod 807 is rotationally connected with a crushing cutter 808; the top end of the outer surface of the first rotating shaft rod 807 is spliced with the second bearing plate 809; the top end of the first rotating shaft rod 807 is rotatably connected with a third bevel gear 8010; the middle part of the rear end of the third bevel gear 8010 is meshed with the fourth bevel gear 8011, and the middle part of the rear end of the fourth bevel gear 8011 is connected with the second bearing plate 809; the right lower part of the fourth bevel gear 8011 is in transmission connection with the fifth bevel gear 8012; the middle part of the bottom end of the fifth bevel gear 8012 is meshed with the sixth bevel gear 8013; the middle part of the bottom end of the sixth bevel gear 8013 is rotationally connected with the second rotating shaft rod 8014; the bottom end of the second rotating shaft rod 8014 is spliced with the connecting inclined rod 8015; the bottom end of the connecting inclined rod 8015 is spliced with the grinding ball 8016; a special-shaped mortar 8017 is arranged below the grinding ball 8016; the right side of the special-shaped mortar 8017 is provided with a sampling valve 8018; the outer surface of the sampling valve 8018 is connected with the first connecting cabin plate 3; the bottom end of the special-shaped mortar 8017 is connected with the machine body bottom plate 1; the bottom end of the crushing cabin 805 is connected with the machine body bottom plate 1; the middle part of the bottom end of the flushing flow channel plate 804 is connected with the machine body bottom plate 1; the top end of the outer surface of the blanking pipe 801 is connected with the top cabin board 2; the right end of the second bearing plate 809 is connected with the second connecting cabin plate 4; the lower side of the outer surface of the second rotating shaft 8014 is connected with the second connecting deck 4; the left upper part of the fourth bevel gear 8011 is connected with the second bevel gear 7010; the left lower part of the fourth bevel gear 8011 is connected with the first driving wheel 602; the middle part of the rear end of the fifth bevel gear 8012 is connected with the second connection deck 4.
Firstly, the withdrawing mechanism 704 puts samples on the top end of the flushing flow channel plate 804, then sufficient cell lysis solution is added through the blanking pipe 801, then the cell lysis solution is sprayed out from the steering nozzle 802 to the inner side of the flushing flow channel plate 804, the extracted samples on the inner side of the flushing flow channel plate 804 are flushed and carried to the inner side of the crushing cabin 805, meanwhile, the first driving wheel 602 drives the fourth bevel gear 8011 to rotate, then the fourth bevel gear 8011 drives the third bevel gear 8010 to rotate, then the third bevel gear 8010 drives the first rotating shaft 807 to rotate, the first rotating shaft 807 drives the crushing cutter 808 to grind the cell mixing solution, then the valve at the bottom of the right end of the crushing cabin 805 is controlled to be opened, then the cell lysis solution in the crushing cabin 805 enters the inner side of the top end of the special-shaped mortar 8017, the fourth bevel gear 8011 drives the second rotating shaft 8014 to rotate, then the bottom end of the second rotating shaft 8014 drives the grinding ball 8016 to rotate on the inner side of the top of the special-shaped mortar 8017 through the connecting bevel shaft 8015, the cell lysis solution at the inner side of the special-shaped mortar 8017 is further opened, and then the cell tissue sample is further ground, and the cell tissue sample is further ground.
The external sealing communication mechanism 9 comprises a screwing sealing cover 901, an outer cylinder 902, a second dead-angle-free ultraviolet emitter 903, an embedded cylinder 904 and a latex sleeve 905; the lower side of the outer surface of the screwing sealing cover 901 is screwed with the connecting outer cylinder 902; a plurality of groups of second dead-angle-free ultraviolet emitters 903 are equidistantly arranged on the circumference of the outer ring at the top end of the connecting outer cylinder 902; the bottom end of the connecting outer cylinder 902 is sleeved with the embedded cylinder 904; the middle part of the bottom end of the embedded cylinder 904 is bonded with a latex sleeve 905; the outer surface of the embedded cylinder 904 is connected with the third connection deck 5.
When the glove is used, the screwed sealing cover 901 is firstly taken down, then the power supply of the second dead-angle-free ultraviolet emitter 903 is connected, the hand and the arm are sterilized, then the outer cylinder 902 and the embedded cylinder 904 are connected to extend downwards into the arm, the latex sleeve 905 is sleeved on the arm for aseptic package protection, and then the hand is exposed from the bottom end of the latex sleeve 905 for internal operation.
The withdrawing mechanism 704 comprises a third electric sliding block 70401, an outer connecting ring plate 70402, a sampling tube 70403, a sampling large-caliber needle 70404, a withdrawing piston 70405, a piston connecting rod 70406, an internal threaded slip ring 70407, a screw rod 70408 and a fifth flat gear 70409; the top of the front end of the third electric sliding block 70401 is spliced with a connecting outer ring plate 70402; the bottom end of the outer ring plate 70402 is connected with a sampling tube 70403 for welding; the middle part of the bottom end of the sampling tube 70403 is spliced with a sampling large-caliber needle 70404; the inner surface of the sampling tube 70403 is in sliding connection with the drawing-out piston 70405; the middle part of the top end of the drawing piston 70405 is spliced with a piston connecting rod 70406; the top of the rear end of the piston connecting rod 70406 is connected with an internal thread slip ring 70407 through bolts; the inner surface of the internally threaded slip ring 70407 is rotationally connected with the screw rod 70408, and the top end and the bottom end of the outer surface of the screw rod 70408 are connected with the third electric sliding block 70401; the middle part of the top end of the screw rod 70408 is rotationally connected with a fifth flat gear 70409; the middle part of the left end of the fifth flat gear 70409 is connected with the first flat gear 703; the middle part of the rear end of the third electric sliding block 70401 is connected with a second electric sliding rail 705.
Firstly, the third electric sliding rail 707 drives the second electric sliding rail 705 to communicate with the withdrawing mechanism 704 through the second electric sliding block 706 to move downwards, then the withdrawing mechanism 704 stretches into the tissue sample to extract, namely, the sampling large-caliber needle 70404 enters into the tissue sample, then the first flat gear 703 drives the fifth flat gear 70409 to rotate, then the fifth flat gear 70409 rotates to drive the screw rod 70408 to rotate, then the screw rod 70408 rotates to drive the internal thread sliding ring 70407 to move upwards, then the internal thread sliding ring 70407 drives the withdrawing piston 70405 to slide upwards on the inner side of the sampling cylinder 70403 through the piston connecting rod 70406, the internal air pressure of the sampling cylinder 70403 is reduced, then the tissue sample enters into the sampling cylinder 70403 through the sampling large-caliber needle 70404 to finish sampling, then the withdrawing mechanism 704 moves to the meshing position of the right fifth flat gear 70409 and the fourth flat gear 7013, then the fourth flat gear 7013 drives the screw rod 70408 to rotate reversely, the screw rod 70408 rotates to drive the internal thread 70407 to move downwards, and then the internal thread sliding ring 70407 drives the withdrawing piston 70405 to slide upwards through the piston connecting rod 70406, the internal air pressure of the sampling cylinder is reduced, the tissue sample is blown out of the sampling cylinder 638 through the sampling large-caliber needle and the sampling mechanism is increased, and the tissue sample is blown out of the sampling mechanism is further increased.
The external sealing communication mechanism 9 is provided with three groups, which are respectively positioned in the middle of the third connecting deck plate 5, the left side in the top end of the top deck plate 2 and the middle of the left end of the top deck plate 2, and the same external sealing communication mechanism 9 positioned in the middle of the left end of the top deck plate 2 is not provided with a latex sleeve 905, wherein the two groups of the middle of the third connecting deck plate 5 and the left side in the top end of the top deck plate 2 are operation inlets prepared for manual operation of a worker, and one group of the middle of the left end of the top deck plate 2 is used for adding prepared samples into the sample sampling vessel 6015.
The groove at the top of the shaped mortar 8017 is configured to be in a ring shape that is attached to the grinding ball 8016 when it rotates, so that the grinding ball 8016 can be ground with the shaped mortar 8017 in a tight and seamless manner.
The right end of the second electric sliding rail 705 is provided with a bar-shaped auxiliary damping sliding rail so as to ensure that the drawing-off mechanism 704 keeps horizontal in the up-and-down movement process.
The length of the first flat gear 703 is twice the length of the fifth flat gear 70409 so that the inside operation of the withdrawing mechanism 704 can be maintained during the up-and-down movement.
The foregoing description of the embodiments has been provided for the purpose of illustrating the general principles of the invention, and is not meant to limit the scope of the invention, but to limit the invention to the particular embodiments, and any modifications, equivalents, improvements, etc. that fall within the spirit and principles of the invention are intended to be included within the scope of the invention.

Claims (7)

1. The tissue treatment device for protein purification comprises a bottom plate (1), a top cabin plate (2) and a first connecting cabin plate (3), and is characterized by further comprising a second connecting cabin plate (4), a third connecting cabin plate (5), an isolation sampling mechanism (6), an internal tissue sampling mechanism (7), a tissue cell crushing mechanism (8), an external sealing communication mechanism (9) and an operation control screen (10); the left side of the top end of the machine body bottom plate (1) is welded with the top cabin plate (2); the right side of the top end of the machine body bottom plate (1) is welded with the first connecting cabin plate (3); the left side of the top end of the machine body bottom plate (1) is connected with an isolation sampling mechanism (6), and the left middle part of the isolation sampling mechanism (6) is connected with a top cabin plate (2); the right side of the top end of the machine body bottom plate (1) is connected with a tissue cell crushing mechanism (8), the right lower part of the tissue cell crushing mechanism (8) is connected with a first connecting cabin plate (3), and the left side of the tissue cell crushing mechanism (8) is connected with an isolation sampling mechanism (6); the middle part of the inner top end of the top cabin plate (2) is connected with an internal tissue sampling mechanism (7), the bottom of the left end of the internal tissue sampling mechanism (7) is connected with an isolation sampling mechanism (6), and the right lower part of the internal tissue sampling mechanism (7) is connected with a tissue cell crushing mechanism (8); the right side of the inner top end of the top cabin plate (2) is welded with a third connecting cabin plate (5), and the middle lower part of the left end of the third connecting cabin plate (5) is connected with an internal tissue sampling mechanism (7); the top of the left end of the first connecting cabin plate (3) is welded with the second connecting cabin plate (4), the left part of the second connecting cabin plate (4) is connected with the tissue cell crushing mechanism (8), and the middle part of the top end of the second connecting cabin plate (4) is connected with the third connecting cabin plate (5); the middle upper part of the right end of the third connecting cabin plate (5) is sleeved with an external sealing communication mechanism (9); an operation control screen (10) is arranged at the lower side of the right end of the third connecting cabin plate (5);
The isolation sampling mechanism (6) comprises a power motor (601), a first driving wheel (602), a first spring rod (603), a belt control wheel (604), a second driving wheel (605), a first driving gear (606), a first electric push rod (607), a toothed bar (608), a limit sliding rail (609), a buffer limiting plate (6010), a buffer spring (6011), a damping slip ring (6012), a first electric sliding rail (6013), a first electric sliding block (6014), a sample sampling vessel (6015), a partition plate (6016), a top clamping plate (6017), a first dead-angle-free ultraviolet emitter (6018) and a first frame plate (6019); the middle part of the front end of the power motor (601) is rotationally connected with a first driving wheel (602); a first spring rod (603) is arranged at the upper left of the power motor (601); the right upper part of the first driving wheel (602) is in driving connection with a second driving wheel (605); the right end of the first spring rod (603) is rotationally connected with a belt control wheel (604) through a bearing; the middle part of the front end of the second driving wheel (605) is rotationally connected with the first driving gear (606); the middle part of the rear end of the second driving wheel (605) is rotationally connected with the first electric push rod (607) through a bearing; the middle part of the right end of the first transmission gear (606) is meshed with a toothed bar (608); the lower side of the right end of the toothed bar (608) is in sliding connection with a limit sliding rail (609); the top end of the toothed bar (608) is spliced with the partition plate (6016); the middle lower part of the left end of the limit sliding rail (609) is in sliding connection with a buffer limit plate (6010); the bottom end of the buffering limiting plate (6010) is connected with a buffering spring (6011); the bottom of the outer surface of the isolation plate (6016) is in sliding connection with the damping slip ring (6012); the top end of the damping slip ring (6012) is connected with the first electric sliding rail (6013) through bolts; the left side of the top end of the first electric sliding rail (6013) is in sliding connection with the first electric sliding block (6014); the right end of the first electric sliding rail (6013) is connected with the first frame plate (6019); the top end of the first electric sliding block (6014) is sleeved with a sample sampling vessel (6015); the top end of the isolation plate (6016) is spliced with the top clamping plate (6017); the right side of the bottom end of the top clamping plate (6017) is provided with a first dead-angle-free ultraviolet emitter (6018); the bottom end of the first frame plate (6019) is connected with the machine body bottom plate (1); the left end of the top clamping plate (6017) is connected with the top cabin plate (2); the right end of the top clamping plate (6017) is connected with an internal tissue sampling mechanism (7); the left end of the first electric sliding rail (6013) is connected with the top cabin board (2); the left end of the first electric push rod (607) is connected with the top cabin board (2); the left end of the first spring rod (603) is connected with the top cabin plate (2); the bottom end of the power motor (601) is connected with the top cabin plate (2); the bottom end of the buffer spring (6011) is connected with the machine body bottom plate (1); the bottom end of the limit sliding rail (609) is connected with the machine body bottom plate (1); the upper right side of the first driving wheel (602) is connected with a tissue cell disruption mechanism (8);
The internal tissue sampling mechanism (7) comprises a third driving wheel (701), a first gear plate (702), a first flat gear (703), a withdrawing mechanism (704), a second electric sliding rail (705), a second electric sliding block (706), a third electric sliding rail (707), a fourth driving wheel (708), a first bevel gear (709), a second bevel gear (7010), a second flat gear (7011), a third flat gear (7012) and a fourth flat gear (7013); the middle part of the top end of the third driving wheel (701) is rotationally connected with the first gear plate (702) through a bearing; the middle part of the bottom end of the third driving wheel (701) is rotationally connected with the first flat gear (703); the right side of the third driving wheel (701) is in driving connection with a fourth driving wheel (708), and the top end of the outer surface of the fourth driving wheel (708) is connected with the first gear plate (702); the middle part of the right end of the first flat gear (703) is meshed with the withdrawing mechanism (704); the bottom of the rear end of the extraction mechanism (704) is in sliding connection with a second electric sliding rail (705); the left end of the second electric sliding rail (705) is connected with a second electric sliding block (706) through a bolt; the rear end of the second electric sliding block (706) is in sliding connection with a third electric sliding rail (707); the middle part of the top end of the fourth driving wheel (708) is rotationally connected with a first bevel gear (709); the middle part of the rear end of the first bevel gear (709) is meshed with the second bevel gear (7010), and the middle part of the rear end of the second bevel gear (7010) is connected with the first gear plate (702); the middle part of the right end of the second flat gear (7011) is meshed with the third flat gear (7012), and the middle part of the top end of the third flat gear (7012) is connected with the first gear plate (702); the middle part of the bottom end of the third flat gear (7012) is rotationally connected with the fourth flat gear (7013); the top end of the first gear plate (702) is connected with the top cabin plate (2); the right lower part of the second bevel gear (7010) is connected with a tissue cell disruption mechanism (8); the right end of the second electric sliding rail (705) is connected with a third connecting cabin board (5); the middle part of the left end of the third electric sliding rail (707) is connected with a top clamping plate (6017);
The tissue cell crushing mechanism (8) comprises a blanking pipe (801), a steering nozzle (802), a nozzle frame (803), a flushing flow channel plate (804), a crushing cabin (805), a first bearing strip (806), a first rotating shaft rod (807), a crushing cutter (808), a second bearing plate (809), a third bevel gear (8010), a fourth bevel gear (8011), a fifth bevel gear (8012), a sixth bevel gear (8013), a second rotating shaft rod (8014), a connecting diagonal rod (8015), a grinding ball (8016), a special-shaped mortar (8017) and a sampling valve (8018); the bottom end of the blanking pipe (801) is spliced with the steering nozzle (802); the middle part of the outer surface of the steering nozzle (802) is sleeved with a nozzle frame (803); the bottom of the right end of the nozzle frame (803) is connected with a flushing flow channel plate (804); a crushing cabin (805) is arranged at the right lower part of the flushing flow channel plate (804); the inner middle part of the crushing cabin (805) is provided with a first bearing strip (806); the middle part of the first bearing strip (806) is spliced with the first rotating shaft rod (807); the middle part of the bottom end of the first rotating shaft rod (807) is rotationally connected with a mincing knife (808); the top end of the outer surface of the first rotating shaft rod (807) is spliced with the second bearing plate (809); the top end of the first rotating shaft rod (807) is rotationally connected with a third bevel gear (8010); the middle part of the rear end of the third bevel gear (8010) is meshed with a fourth bevel gear (8011), and the middle part of the rear end of the fourth bevel gear (8011) is connected with a second bearing plate (809); the right lower part of the fourth bevel gear (8011) is in transmission connection with the fifth bevel gear (8012); the middle part of the bottom end of the fifth bevel gear (8012) is meshed with the sixth bevel gear (8013); the middle part of the bottom end of the sixth bevel gear (8013) is rotationally connected with a second rotating shaft lever (8014); the bottom end of the second rotating shaft rod (8014) is spliced with the connecting inclined rod (8015); the bottom end of the connecting inclined rod (8015) is spliced with the grinding ball (8016); a special-shaped mortar (8017) is arranged below the grinding ball (8016); the right side of the special-shaped mortar (8017) is provided with a sampling valve (8018); the outer surface of the sampling valve (8018) is connected with the first connecting cabin plate (3); the bottom end of the special-shaped mortar (8017) is connected with the machine body bottom plate (1); the bottom end of the crushing cabin (805) is connected with the machine body bottom plate (1); the middle part of the bottom end of the flushing flow channel plate (804) is connected with the machine body bottom plate (1); the top end of the outer surface of the blanking pipe (801) is connected with the top cabin plate (2); the right end of the second bearing plate (809) is connected with the second connecting cabin plate (4); the lower side of the outer surface of the second rotating shaft lever (8014) is connected with the second connecting cabin plate (4); the upper left part of the fourth bevel gear (8011) is connected with the second bevel gear (7010); the left lower part of the fourth bevel gear (8011) is connected with the first driving wheel (602); the middle part of the rear end of the fifth bevel gear (8012) is connected with the second connecting cabin plate (4).
2. The tissue treatment device for protein purification according to claim 1, wherein the external sealing and communicating mechanism (9) comprises a screwing sealing cover (901), an outer cylinder (902), a second dead-angle-free ultraviolet emitter (903), an embedding cylinder (904) and a latex sleeve (905); the lower side of the outer surface of the screwing sealing cover (901) is screwed with the connecting outer cylinder (902); a plurality of groups of second dead-angle-free ultraviolet emitters (903) are equidistantly arranged on the circumference of the outer ring at the top end of the connecting outer cylinder (902); the bottom end of the connecting outer cylinder (902) is sleeved with the embedded cylinder (904); the middle part of the bottom end of the embedded cylinder (904) is bonded with the latex sleeves (905); the outer surface of the embedded cylinder (904) is connected with a third connecting cabin plate (5).
3. The tissue treatment device for protein purification according to claim 2, wherein the extraction mechanism (704) comprises a third electric slider (70401), an outer ring plate (70402) connected, a sampling tube (70403), a sampling large-caliber needle (70404), an extraction piston (70405), a piston connecting rod (70406), an internally threaded slip ring (70407), a screw (70408) and a fifth flat gear (70409); the top of the front end of the third electric sliding block (70401) is spliced with the connecting outer ring plate (70402); the bottom end of the outer ring plate (70402) is connected with a sampling tube (70403) for welding; the middle part of the bottom end of the sampling tube (70403) is spliced with a sampling large-caliber needle head (70404); the inner surface of the sampling tube (70403) is in sliding connection with the drawing-out piston (70405); the middle part of the top end of the drawing and discharging piston (70405) is spliced with a piston connecting rod (70406); the top of the rear end of the piston connecting rod (70406) is connected with an internal thread slip ring (70407) through bolts; the inner surface of the internal thread slip ring (70407) is rotationally connected with the screw rod (70408), and the top end and the bottom end of the outer surface of the screw rod (70408) are connected with a third electric sliding block (70401); the middle part of the top end of the screw rod (70408) is rotationally connected with a fifth flat gear (70409); the middle part of the left end of the fifth flat gear (70409) is connected with the first flat gear (703); the middle part of the rear end of the third electric sliding block (70401) is connected with a second electric sliding rail (705).
4. A tissue treatment device for purifying proteins according to claim 3, wherein three sets of external sealing communication mechanisms (9) are provided, each set being located in the middle of the third connecting deck (5), the left side in the top end of the top deck (2) and the middle of the left end of the top deck (2), and the same external sealing communication mechanism (9) being located in the middle of the left end of the top deck (2) is not provided with a latex sleeve (905).
5. The tissue treatment device for protein purification according to claim 4, wherein the groove shape at the top end of the shaped mortar (8017) is formed in a ring shape which is kept in contact with the grinding ball (8016) when it is rotated.
6. The tissue treatment device for protein purification according to claim 5, wherein a bar-shaped auxiliary damping slide rail is provided at the right end of the second electric slide rail (705).
7. The tissue treatment device for protein purification according to claim 6, wherein the length of the first flat gear (703) is twice the length of the fifth flat gear (70409).
CN202010636182.7A 2020-07-04 2020-07-04 Tissue treatment device for protein purification Active CN111811863B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010636182.7A CN111811863B (en) 2020-07-04 2020-07-04 Tissue treatment device for protein purification

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010636182.7A CN111811863B (en) 2020-07-04 2020-07-04 Tissue treatment device for protein purification

Publications (2)

Publication Number Publication Date
CN111811863A CN111811863A (en) 2020-10-23
CN111811863B true CN111811863B (en) 2024-03-26

Family

ID=72855414

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010636182.7A Active CN111811863B (en) 2020-07-04 2020-07-04 Tissue treatment device for protein purification

Country Status (1)

Country Link
CN (1) CN111811863B (en)

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4505433A (en) * 1982-09-28 1985-03-19 Selenke William M Tissue grinding and transporting device
WO2004073846A1 (en) * 2003-02-19 2004-09-02 The Horticulture And Food Research Institute Of New Zealand Limited Tissue disintegrator and sample processing system
CN203803511U (en) * 2014-03-28 2014-09-03 奥星衡迅生命科技（上海）有限公司 Sterile separation device
WO2015161057A1 (en) * 2014-04-18 2015-10-22 Auxocell Laboratories, Inc. Systems and methods for processing cells
CN105943382A (en) * 2016-05-18 2016-09-21 张巍 Sterile dispensing system
CN108714440A (en) * 2018-06-26 2018-10-30 杨兰婷 A kind of superclean bench of use for laboratory
CN109431553A (en) * 2018-12-29 2019-03-08 宋超 A kind of oncology clinic biopsy sample sampler
CN109609608A (en) * 2019-01-17 2019-04-12 浙江大学 The linear quick dual temperature PCR amplification automatic control device of one kind and control method
CN109821600A (en) * 2019-03-11 2019-05-31 四川省农业科学院分析测试中心 A kind of multitube automatic grinding device
CN110257241A (en) * 2019-07-04 2019-09-20 北京晶科瑞医学检验实验室有限公司 A kind of device of the quick separating tumour cell from lung's malignant hydrothorax
CN110369080A (en) * 2019-07-02 2019-10-25 鄂州职业大学 A kind of multifunctional drug grinding device
CN209816003U (en) * 2019-01-30 2019-12-20 广州康颐堂医疗用品有限公司 Protein extraction device
CN210891980U (en) * 2019-11-12 2020-06-30 朱晓菲 Sterilization instrument for operating room nursing

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108126783B (en) * 2018-02-08 2023-09-29 吉林大学 Grinding device for traditional Chinese medicine

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4505433A (en) * 1982-09-28 1985-03-19 Selenke William M Tissue grinding and transporting device
WO2004073846A1 (en) * 2003-02-19 2004-09-02 The Horticulture And Food Research Institute Of New Zealand Limited Tissue disintegrator and sample processing system
CN203803511U (en) * 2014-03-28 2014-09-03 奥星衡迅生命科技（上海）有限公司 Sterile separation device
WO2015161057A1 (en) * 2014-04-18 2015-10-22 Auxocell Laboratories, Inc. Systems and methods for processing cells
CN105943382A (en) * 2016-05-18 2016-09-21 张巍 Sterile dispensing system
CN108714440A (en) * 2018-06-26 2018-10-30 杨兰婷 A kind of superclean bench of use for laboratory
CN109431553A (en) * 2018-12-29 2019-03-08 宋超 A kind of oncology clinic biopsy sample sampler
CN109609608A (en) * 2019-01-17 2019-04-12 浙江大学 The linear quick dual temperature PCR amplification automatic control device of one kind and control method
CN209816003U (en) * 2019-01-30 2019-12-20 广州康颐堂医疗用品有限公司 Protein extraction device
CN109821600A (en) * 2019-03-11 2019-05-31 四川省农业科学院分析测试中心 A kind of multitube automatic grinding device
CN110369080A (en) * 2019-07-02 2019-10-25 鄂州职业大学 A kind of multifunctional drug grinding device
CN110257241A (en) * 2019-07-04 2019-09-20 北京晶科瑞医学检验实验室有限公司 A kind of device of the quick separating tumour cell from lung's malignant hydrothorax
CN210891980U (en) * 2019-11-12 2020-06-30 朱晓菲 Sterilization instrument for operating room nursing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
青岛浒苔完整叶绿体分离及其蛋白质提取方法的初步建立;杜智欣等;植物检疫(第02期);第44-48页 *

Also Published As

Publication number Publication date
CN111811863A (en) 2020-10-23

Similar Documents

Publication Publication Date Title
CN103911366A (en) Genome DNA extraction method and its kit
CN111811863B (en) Tissue treatment device for protein purification
CN106244455B (en) Fully-automatic intelligent cell culture apparatus and its control method
CN112414814A (en) Sample extraction element for plant quarantine
CN2864657Y (en) Automatic sampling valve
CN209613265U (en) Manipulator for the clamping of bottle centrifuge tubing consumptive material
CN107213168A (en) Skin disease nursing for treating product based on marine organism extract and preparation method thereof
CN205088264U (en) Carry high absorptivity's leather rotating drum
CN207726711U (en) Cryopreservation tube batch hood-opening device
CN108034569B (en) Clinical laboratory's bacterial incubator
CN211937219U (en) Pharmacy is with continuous traditional chinese medicine extraction device of press type
CN115747057A (en) Bone marrow mesenchymal stem cell isolated culture device
CN1961981A (en) Method for aseptic production of animal raw serum in full sealing environment by multi-linked blood bag
CN214361344U (en) Microbial agent apparatus for producing
CN113072613B (en) Insect virus schizolysis polypeptide extraction element
CN211024294U (en) Pipettor degassing unit
CN210458153U (en) Microbial fermentation tank convenient to feed supplement
CN212100296U (en) Raw milk temporary storage device
CN111320669A (en) Thick wall microbial protein extraction element
CN102816704A (en) Medium inoculation technology and its special equipment in Cordyceps cicadae solid fermentation industry
CN202005883U (en) Sterile cotton ball tank for department of stomatology
CN217781167U (en) Quick nucleic acid extraction instrument
CN1316511A (en) Process and equipment for preparing liquid strain of edible fungus
CN204925697U (en) Separating culture control box is preserved to portable pathogen
CN2814390Y (en) Ice bathing homogenizer

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20240417

Address after: Room 403, 1st Floor, Building B1, Phase I, Changsha E Center, No. 18 Xiangtai Road, Liuyang Economic and Technological Development Zone, Changsha City, Hunan Province, 410329

Patentee after: Hunan Possible Biotechnology Co.,Ltd.

Country or region after: China

Address before: 423000 Room 201, No. 28-7, Jiefang Road, Beihu District, Zhuzhou City, Hunan Province

Patentee before: Li Qinghua

Country or region before: China