CN111802407A - Composition and preparation and application thereof - Google Patents
Composition and preparation and application thereof Download PDFInfo
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- CN111802407A CN111802407A CN202010497720.9A CN202010497720A CN111802407A CN 111802407 A CN111802407 A CN 111802407A CN 202010497720 A CN202010497720 A CN 202010497720A CN 111802407 A CN111802407 A CN 111802407A
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- polylysine
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- lysozyme
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D31/00—Materials specially adapted for outerwear
- A41D31/04—Materials specially adapted for outerwear characterised by special function or use
- A41D31/30—Antimicrobial, e.g. antibacterial
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/11—Compounds containing epoxy groups or precursors thereof
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/322—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing nitrogen
- D06M13/402—Amides imides, sulfamic acids
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/19—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with synthetic macromolecular compounds
- D06M15/37—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M15/59—Polyamides; Polyimides
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D2500/00—Materials for garments
- A41D2500/30—Non-woven
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Abstract
The invention discloses a composition, and a preparation method and application thereof. The disinfectant solution prepared by using the composition provided by the invention is a phosphate buffer solution with pH of 6.8 and containing 0.2g/L of polylysine, 0.8-1.0g/L of N-acetylneuraminic acid, 1.0g/L of lysozyme and 10g/L of trehalose. The disinfectant solution can be used for preparing protective non-woven fabrics and further used for preparing microbial protective articles such as masks and the like. Good protection effect, good stability and long storage life.
Description
Technical Field
The invention relates to a composition, and a preparation method and application thereof.
Background
The new crown pneumonia epidemic situation makes human body more aware of the severity of threat of microorganisms such as virus and bacteria to human body. The conventional sterilization and disinfection in daily life and the supply and effectiveness of protective equipment under special conditions become hot points of attention of people, the improvement of the effectiveness of conventional sterilization and disinfection products for daily life becomes an urgent need of people, and the timely and effective supply and the improvement of the effectiveness of protection of protective equipment such as masks, medical protective clothing and the like also become urgent needs and key factors for epidemic prevention and control.
Disclosure of Invention
The invention aims to provide a composition, and preparation and application thereof, so as to at least partially improve the effectiveness of sterilization and disinfection products, improve the antibacterial and virus adsorption capacity of microbial protective products such as masks and the like, and improve the effective supply capacity of the microbial protective products such as masks and the like by prolonging the shelf life of the products.
The present invention relates to at least the following technical solutions.
The embodiment of the invention relates to a composition, which comprises 1 part of polylysine, 4-5 parts of N-acetylneuraminic acid, 10 parts of lysozyme and a proper amount of trehalose. Preferably, the composition comprises 1 part of polylysine, 5 parts of N-acetylneuraminic acid, 10 parts of lysozyme and a proper amount of trehalose.
The embodiment of the invention relates to a disinfection solution which is a phosphate buffer solution containing 0.2g/L of polylysine, 0.8-1.0g/L of N-acetylneuraminic acid, 1.0g/L of lysozyme and 10g/L of trehalose and having the pH value of 6.8. The disinfecting solution may be prepared using the aforementioned composition.
The embodiment of the invention relates to a protective non-woven fabric which simultaneously bears a proper amount of polylysine, N-acetylneuraminic acid, lysozyme and trehalose. Specifically, it may be such that 1.6mg of polylysine, 6.4 to 8.0mg of N-acetylneuraminic acid, 8.0mg of lysozyme and 80mg of trehalose are carried per square meter of nonwoven fabric.
The embodiment of the invention also provides a method for preparing the protective non-woven fabric, which comprises the steps of diluting the disinfection solution by 2 times with 15% alcohol, uniformly spraying the disinfection solution according to the total amount of 8mL per square meter of non-woven fabric at normal temperature, and drying at 60 ℃.
The embodiment of the invention also relates to a microbial protection product which comprises at least one layer of the protection non-woven fabric. The microbial protective product refers to a protective product which can be worn or worn by people to prevent microbial infection such as virus and bacteria, and comprises a mask, a medical protective garment and the like.
The invention also relates to the use of the aforementioned composition for the preparation of a disinfecting solution.
The invention also relates to the application of the disinfection solution in preparing the protective non-woven fabric.
The invention also relates to application of the protective non-woven fabric in preparation of microbial protective articles such as masks and the like.
The antibacterial and disinfectant composition provided by the invention has obviously improved antibacterial and disinfectant capabilities compared with the existing like products, and has long storage life. The antibacterial sterilizing composition is sprayed on non-woven fabrics to manufacture the mask, has obvious advantages in antibacterial capability and virus adsorption capability compared with the prior similar products, has longer retention period, and is more guaranteed in the supply capability of responding to emergencies.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
The percentage content of the invention is volume ratio content under the condition that the solvent and the solute are both liquid, and weight volume ratio content under the condition that the solute is solid solvent.
First group
Example 1
The inventors provide formulation 1:
1 part of polylysine and 3 parts of N-acetylneuraminic acid.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with a final concentration of 0.2g/L, and adjusting the pH to 6.8.
Example 2
The inventors provide formulation 2:
1 part of polylysine, 3 parts of N-acetylneuraminic acid, 10 parts of chitosan and 10 parts of lysozyme.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with a final concentration of 0.2g/L, and adjusting the pH to 6.8.
Example 3
The inventors provide formulation 3:
1 part of polylysine, 3 parts of N-acetylneuraminic acid, 8 parts of lysozyme and a proper amount of trehalose.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with the final concentration of 0.2g/L, simultaneously adding trehalose to 10g/L, and adjusting the pH to 6.8.
Example 4
The inventors provide formulation 4:
1 part of polylysine, 4 parts of N-acetylneuraminic acid and a proper amount of 8 parts of lysozyme and trehalose.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with the final concentration of 0.2g/L, simultaneously adding trehalose to 10g/L, and adjusting the pH to 6.8.
Example 5
The inventors provide formulation 5:
1 part of polylysine, 5 parts of N-acetylneuraminic acid, 8 parts of lysozyme and a proper amount of trehalose.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with the final concentration of 0.2g/L, simultaneously adding trehalose to 10g/L, and adjusting the pH to 6.8.
Example 6
The inventors provide formulation 6:
1 part of polylysine, 3 parts of N-acetylneuraminic acid, 10 parts of lysozyme and a proper amount of trehalose.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with the final concentration of 0.2g/L, simultaneously adding trehalose to 10g/L, and adjusting the pH to 6.8.
Example 7
The inventors provide formulation 7:
1 part of polylysine, 4 parts of N-acetylneuraminic acid, 10 parts of lysozyme and a proper amount of trehalose.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with the final concentration of 0.2g/L, simultaneously adding trehalose to 10g/L, and adjusting the pH to 6.8.
Example 8
The inventors provide formulation 8:
1 part of polylysine, 5 parts of N-acetylneuraminic acid, 10 parts of lysozyme and a proper amount of trehalose.
Weighing the components in proportion, adding phosphate buffer solution to prepare a solution of polylysine with the final concentration of 0.2g/L, simultaneously adding trehalose to 10g/L, and adjusting the pH to 6.8.
Each of the solutions of the formulations provided in examples 1 to 8 was sprayed on LB medium of Escherichia coli, blood plate of Staphylococcus aureus, and Sasa's dextrose agar medium of Candida albicans, respectively, and the growth of a visible colony was observed after 48 hours for each of the media of examples.
By comparison of the results, colonies were visible to the naked eye in examples 1 to 4, and were not detected in examples 5 to 8. The bacteriostatic effect of the patent in examples 5-8 is proved to be better.
Example 9: the formulation of example 5 was sprayed onto a nonwoven fabric to prepare a repellent nonwoven fabric.
The spraying process parameters are as follows: diluting the solution with 15% alcohol by 2 times, uniformly spraying at normal temperature according to the total amount of 5mL per square meter of non-woven fabric, and drying at 60 ℃.
Example 10: the formulation of example 6 was sprayed onto a nonwoven fabric to prepare a repellent nonwoven fabric, according to the spray process of example 9.
Example 11: the formulation of example 7 was sprayed onto a nonwoven fabric to prepare a repellent nonwoven fabric, according to the spray process of example 9.
Example 12: the formulation of example 8 was sprayed onto a nonwoven fabric to prepare a repellent nonwoven fabric, according to the spray process of example 9.
The nonwoven fabrics prepared in examples 9 to 12 were examined for their adsorption rate to viruses. The solutions formulated in examples 1-8 are defined as biological protection solutions. The experimental procedure can be carried out with reference to the following:
1. conditions of the experiment
1.1 reagents
Influenza virus split vaccine: influenza virus split vaccine (Hualan biological vaccine Co., Ltd., national drug standard S20083016);
enzyme-linked immunosorbent assay (ELISA) kit: H1N1 (swine influenza) hemagglutinin protein ELISA kit (cargo number H1N1-SEK001) and H3N2 (avian influenza) hemagglutinin protein ELISA kit (cargo number H3N2-SEK 11056);
nonwoven cloth material (Xianghe Huaxin nonwoven cloth Co., Ltd., lot number HX-20160830).
1.2 apparatus
A biological protection liquid spraying machine (customized and manufactured by company);
7 100mL beakers;
a homogenizing oscillator (model TS-1, manufactured by Lenbell instruments, Haimen, Inc.);
a vortex oscillator (Qilinbeier XW-80A, manufactured by Ribel instruments, Haimen, Inc.);
an enzyme-labeled analyzer (New technology of Peking Pulang, Inc., model number DNM-9602G).
2. Experimental procedure
2.1 preparation of biological protective Material non-woven Fabric and blank non-woven Fabric
Cutting two pieces of non-woven fabrics with the size of 14cm multiplied by 14cm, diluting the solution by 2 times by using 15% alcohol for 20mL of biological protection solution, uniformly spraying the solution by using a machine high-pressure spraying method according to the amount of 5mL per square meter of non-woven fabrics at normal temperature, drying the solution at 60 ℃ to obtain the non-woven fabrics (effective fabrics for short) serving as biological protection materials, and spraying 5mL of 15% alcohol for blank non-woven fabrics (blank fabrics for short). Each piece of cloth is cut into 4 pieces in half, 3 pieces of the effective cloth are randomly taken and numbered as A1, A2 and A3, 3 pieces of the blank cloth are randomly taken and numbered as B1, B2 and B3, and the surface area of each piece of cloth is 3.5cm multiplied by 3.5 cm;
2.2 preparation of stock influenza Virus vaccine
Taking 3mL (60 mu g/mL) of 6 tributary influenza virus vaccines, adding 147mL of distilled water, diluting to 150mL, mixing uniformly to prepare 2% influenza virus vaccine stock solution with antigen concentration of 1200000 pg/mL;
2.3 preparation of sample solution
2.3.1 6 washed and autoclaved 100mL beakers were added to each beaker, one blank or valid cloth, and 20mL of 2% influenza vaccine stock solution. The number of the blank cloth or the effective cloth is respectively 3, the number of the beaker is corresponding to the number of the cloth material, and the total number of the beaker is 6. No. 7 beaker only added 20mL of 2% vaccine stock solution as positive control;
2.3.2 placing 7 beakers on a homogenizing oscillator, oscillating at constant speed for 30 minutes at room temperature, wherein the rotating speed is 120 rpm;
2.3.3 taking out the cloth soaked in the beaker by using a pair of tweezers, naturally airing, marking and storing, uniformly shaking the residual solution in each beaker, respectively sucking 10 mu L and 1.5mL of EP tubes, adding 990 mu L of sample diluent (0.1% BSA, 20mM Tris,150mM NaCl, pH 7.4), uniformly shaking, diluting by 100 times, and using as the solution for evaluating the adsorption performance of the biological protective material;
2.4 preparation of Standard solution
Sucking 60 mu L of 2% influenza virus vaccine stock solution into a 1.5mL EP tube, adding 540 mu L of sample diluent, uniformly mixing until the volume reaches 600 mu L, diluting at 1:10, taking out 300 mu L of the sample diluent, mixing with 300 mu L of the sample diluent, diluting at 1:20, performing twofold dilution in sequence, and preparing into standard solutions diluted by 1:10, 1:20, 1:40, 1:80, 1:160, 1:320 and 1:640 of the vaccine stock solution, wherein the concentrations are 120000, 60000, 30000, 15000, 7500, 3750 and 1750pg/mL respectively;
2.5 determination of the concentration of influenza Virus antigens by ELISA
2.5.1 coating antibody: diluting primary antibodies in an H1N1(2009 swine influenza) hemagglutinin protein ELISA kit and an H3N2 (avian influenza) hemagglutinin protein ELISA kit to working concentration by using a coating solution (0.05M Na2CO3, NaHCO3, pH9.6), adding the diluted primary antibodies into an ELISA plate, adding 100 mu L of the diluted primary antibodies into each hole, combining the primary antibodies with a solid phase carrier in the ELISA plate, sealing the plate by using a membrane sealing plate, and placing the plate in a 4C refrigerator overnight (16-24 hours);
2.5.2 washing: pouring out the liquid in the wells of the enzyme-labeled plate, adding 350 mu L of washing solution (0.05% Tween20, 20mM Tris,150mM NaCl, pH 7.4), washing for 3 times, and finally patting the plate on a towel or absorbent paper;
2.5.3 blocking: add blocking solution (2% BSA, 20mM Tris,150mM NaCl pH 7.4) 300. mu.L, seal the plate, incubate for 1 hour at room temperature;
2.5.4 washing: the same as 2.5.2;
2.5.5 addition of the sample to be tested: taking out the pre-coated ELISA plate, adding 100 mu L of samples to be detected into each hole, making 4 multiple holes for each sample, sealing the plate with a plate film, and incubating for 2 hours at room temperature;
2.5.6 washing: the same as 2.5.2;
2.5.7 addition of enzyme-labeled secondary antibody: diluting the enzyme-labeled secondary antibody to a proper working concentration, adding 100 mu L of enzyme-labeled secondary antibody into each hole, and incubating for 1 hour at room temperature;
2.5.8 washing: the same as 2.5.2;
2.5.9 adding color developing solution: mixing the color development liquid B and the color development liquid A half an hour before use, placing in a dark place for half an hour, adding 100 mu L of color development liquid into each sample hole, and incubating for 10 minutes in the dark at room temperature;
2.5.10 adding stop solution: mixing well with 50 μ L of each well by vortex oscillator;
2.5.11 Observation of recorded results: recording the absorbance value at the wavelength of 450nm by using an enzyme-linked immunosorbent assay;
2.5.12 Standard Curve is prepared: drawing a sample diluent as a blank, an absorbance A as a horizontal coordinate and an influenza virus vaccine concentration C as a vertical coordinate, and preparing a standard curve to obtain a corresponding formula;
2.5.13 calculating the adsorption rate of the cloth: and (3) bringing the absorbance of the sample solution into a linear formula of a standard curve, and calculating the concentration of the virus antigen in the residual virus vaccine solution after the sample solution is adsorbed by the cloth material.
The adsorption rate Q ═ ((C1 × volume-C2 × dilution times × volume))/((C1 × volume))/((1-C2 × 100/C1) × 100%
Wherein, C1 is the concentration of the virus vaccine stock solution (1200000 pg/mL), C2 is the concentration of the residual virus vaccine solution, the adsorption rate values of the biological protective material group and the blank control group of each experiment are obtained by calculating the arithmetic mean value of the adsorption rates of three effective cloths A1, A2 and A3 and three blank cloths B1, B2 and B3, and the experiments are repeated for three times in total.
3. The result of the detection
By comparing the adsorption efficiency of each example in the group to the virus, the adsorption efficiency of the examples 11 and 12 in the patent is proved to be higher, wherein the adsorption efficiency of the example 12 is the highest.
Example 13: the solution prepared in the formula in example 7 was diluted 2 times with 15% ethanol, uniformly sprayed at room temperature in a total amount of 8mL per square meter of nonwoven fabric, and dried at 60 ℃ to prepare a protective nonwoven fabric.
Example 14: the formulation of example 8 was sprayed onto a nonwoven fabric to prepare a repellent nonwoven fabric, according to the procedure of example 13.
Example 15: the solution prepared in the formula in example 7 was diluted 2 times with 15% ethanol, uniformly sprayed at room temperature in a total amount of 10mL per square meter of nonwoven fabric, and dried at 60 ℃ to prepare a protective nonwoven fabric.
Example 16: the formulation of example 8 was sprayed onto a nonwoven fabric to produce a repellent nonwoven fabric, according to the procedure of example 15.
The virus-adsorbing efficiencies of the nonwoven fabrics for protection prepared in examples 13 to 16 were examined with reference to the above-mentioned experimental methods, and the following results were obtained
By comparison of the results, it was demonstrated that the spray coating process used in examples 13-16 had a higher adsorption efficiency than the previous spray coating process, with examples 13-14 having a higher utilization of the formulated disinfecting solution.
Example 17: stability evaluation of each formulation solution
4 parts of the formula solutions provided in examples 5 to 8 were prepared, sealed in a triangular flask, and taken after 15 days, 30 days, 45 days, and 60 days at 60 ℃ and 50% humidity, respectively, to perform the bacteriostatic test, and the experimental process was as described above. The formulation provided in example 5 showed visible colonies after day 15, the formulation provided in example 6 after day 30, the formulation provided in example 7 after day 60, and the formulation provided in example 8 after day 45. The results demonstrate that the formulations provided in examples 7-8 have better stability, with the best formulation provided in example 7.
Example 18: evaluation of stability of each protective nonwoven Fabric
Experiment 1. the protective nonwoven fabric prepared in example 13 was stored in 4 portions in a sealed state, and taken out at 60 ℃ and 50% humidity for 15 days, 30 days, 45 days, and 60 days, respectively, to examine the virus adsorption efficiency.
Experiment 2: the nonwoven fabric for protection prepared in example 14 was stored in 4 portions in a sealed state, taken out at 60 ℃ and 50% humidity for 15 days, 30 days, 45 days and 60 days, respectively, and the virus adsorption efficiency was examined.
Experiment 3: the nonwoven fabric for protection prepared in example 13 was stored in 4 portions in a sealed state, taken out at 60 ℃ and 20% humidity for 15 days, 30 days, 45 days and 60 days, respectively, and the virus adsorption efficiency was examined.
Experiment 4: the nonwoven fabric for protection prepared in example 14 was stored in 4 portions in a sealed state, taken out at 60 ℃ and 20% humidity for 15 days, 30 days, 45 days and 60 days, respectively, and the virus adsorption efficiency was examined.
| Day 15 | Day 30 | Day 45 | Day 60 | |
| Experiment 1 | 73.2％ | 63.2％ | 47.2％ | 32.2％ |
| Experiment 2 | 76.5％ | 66.1％ | 48.9％ | 38.4％ |
| Experiment 3 | 76.1％ | 65.2％ | 50.2％ | 36.7％ |
| Experiment 4 | 78.9％ | 67.3％ | 52.9％ | 40.8％ |
The results show that the stability of experiments 2 and 4 is the best, which shows that the protective non-woven fabric prepared by diluting the formula solution prepared in example 8 by 2 times with 15% alcohol, uniformly spraying the diluted solution according to the total amount of 8mL per square meter of non-woven fabric at normal temperature, and drying the non-woven fabric at 60 ℃, and the prepared protective non-woven fabric can be more suitable for different storage environments.
Although specific embodiments of the invention have been described in detail, those skilled in the art will appreciate. Various modifications and substitutions of those details may be made in light of the overall teachings of the disclosure, and such changes are intended to be within the scope of the present invention. The full scope of the invention is given by the appended claims and any equivalents thereof.
Claims (10)
1. The composition is characterized by comprising 1 part of polylysine, 4-5 parts of N-acetylneuraminic acid, 10 parts of lysozyme and a proper amount of trehalose.
2. The composition of claim 1, wherein the composition comprises 1 part polylysine, 5 parts N-acetylneuraminic acid, 10 parts lysozyme, and appropriate amounts of trehalose.
3. A disinfecting solution comprising 0.2g/L polylysine, 0.8-1.0g/L N-acetylneuraminic acid, 1.0g/L lysozyme and 10g/L trehalose in a phosphate buffer at a pH of 6.8.
4. The protective non-woven fabric is characterized in that the protective non-woven fabric simultaneously bears polylysine, N-acetylneuraminic acid, lysozyme and trehalose.
5. The nonwoven fabric of claim 4, wherein 1.6mg of polylysine, 6.4-8.0mg of N-acetylneuraminic acid, 8.0mg of lysozyme and 80mg of trehalose are loaded per square meter of nonwoven fabric.
6. The method for preparing the non-woven fabric for protection of any one of claims 4 or 5, which comprises diluting the disinfectant solution prepared in claim 3 with 15% alcohol by 2 times, uniformly spraying the solution at room temperature in a total amount of 8mL per square meter of the non-woven fabric, and drying the solution at 60 ℃.
7. A microbial protective product comprising at least one layer of the protective nonwoven fabric of claim 4 or 5.
8. Use of the composition of claim 1 for the preparation of a disinfecting solution.
9. Use of the antiseptic solution of claim 3 in the preparation of a non-woven protective fabric.
10. Use of a repellent nonwoven fabric according to claim 4 or 5 for the preparation of a microbial protective product.
Priority Applications (1)
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