CN111760018A - Efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and preparation method thereof - Google Patents
Efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and preparation method thereof Download PDFInfo
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- CN111760018A CN111760018A CN202010526277.3A CN202010526277A CN111760018A CN 111760018 A CN111760018 A CN 111760018A CN 202010526277 A CN202010526277 A CN 202010526277A CN 111760018 A CN111760018 A CN 111760018A
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- 239000012530 fluid Substances 0.000 title claims abstract description 37
- 238000011010 flushing procedure Methods 0.000 title claims abstract description 30
- 150000004676 glycans Chemical class 0.000 title claims abstract description 24
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 24
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 24
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 230000003110 anti-inflammatory effect Effects 0.000 title claims abstract description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 33
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 29
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 28
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 28
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 28
- 102000016943 Muramidase Human genes 0.000 claims abstract description 21
- 108010014251 Muramidase Proteins 0.000 claims abstract description 21
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 21
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 21
- 229960000274 lysozyme Drugs 0.000 claims abstract description 20
- 239000004325 lysozyme Substances 0.000 claims abstract description 20
- 239000011780 sodium chloride Substances 0.000 claims abstract description 15
- 206010061218 Inflammation Diseases 0.000 claims abstract description 14
- 239000003755 preservative agent Substances 0.000 claims abstract description 13
- 230000002335 preservative effect Effects 0.000 claims abstract description 13
- 238000005406 washing Methods 0.000 claims abstract description 12
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 8
- 239000012498 ultrapure water Substances 0.000 claims abstract description 8
- 239000008213 purified water Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000006467 substitution reaction Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000002421 anti-septic effect Effects 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 206010052428 Wound Diseases 0.000 abstract description 16
- 208000027418 Wounds and injury Diseases 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 10
- 241000894006 Bacteria Species 0.000 abstract description 7
- 230000035876 healing Effects 0.000 abstract description 6
- 230000001737 promoting effect Effects 0.000 abstract description 6
- 208000031737 Tissue Adhesions Diseases 0.000 abstract description 5
- 230000003467 diminishing effect Effects 0.000 abstract description 5
- 230000004054 inflammatory process Effects 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 5
- 230000004888 barrier function Effects 0.000 abstract description 4
- 230000002349 favourable effect Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 230000029663 wound healing Effects 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 19
- 239000007788 liquid Substances 0.000 description 5
- 210000002421 cell wall Anatomy 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- 229920002683 Glycosaminoglycan Polymers 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000002068 Glycopeptides Human genes 0.000 description 1
- 108010015899 Glycopeptides Proteins 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- MNLRQHMNZILYPY-MDMHTWEWSA-N N-acetyl-alpha-D-muramic acid Chemical compound OC(=O)[C@@H](C)O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O)[C@@H]1NC(C)=O MNLRQHMNZILYPY-MDMHTWEWSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- MBLBDJOUHNCFQT-LXGUWJNJSA-N N-acetylglucosamine Natural products CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 1
- 208000002847 Surgical Wound Diseases 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 229940051921 muramidase Drugs 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/716—Glucans
- A61K31/717—Celluloses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/14—Alkali metal chlorides; Alkaline earth metal chlorides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01017—Lysozyme (3.2.1.17)
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Surgery (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Dermatology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a high-efficiency antibacterial and anti-inflammatory biological polysaccharide flushing fluid and a preparation method thereof, wherein the high-efficiency antibacterial and anti-inflammatory biological polysaccharide flushing fluid comprises, by weight, 0.5% -6% of sodium carboxymethylcellulose, 0.3% -5% of lysozyme, 0.1% -0.9% of sodium chloride, 0.1% -0.3% of a preservative, 0.01% -0.1% of an acid-base regulator and the balance ultrapure water. According to the invention, the lysozyme is added into the washing fluid component prepared from the sodium carboxymethylcellulose as the raw material, so that the effects of efficiently inhibiting bacteria and diminishing inflammation and promoting rapid healing of the wound are increased while the wound is cleaned and tissue adhesion is prevented, and a physical barrier can be finally formed to provide a favorable growth environment for wound healing.
Description
Technical Field
The invention relates to a wound flushing fluid and a preparation method thereof, in particular to a high-efficiency antibacterial and anti-inflammatory biological polysaccharide flushing fluid and a preparation method thereof, and belongs to the field of medical instruments.
Background
In the surgical operation, the wound surface or the incision is treated by using normal saline or a flushing fluid prepared by mixing sodium carboxymethylcellulose with traditional Chinese medicine components, chitosan or other saccharides in most clinical cases. Compared with normal saline, the washing liquid has the effects of increasing adhesion resistance and the like, but provides a healing environment for the wound, and the components in the washing liquid have no outstanding effects of inhibiting bacteria, diminishing inflammation and promoting the wound to heal quickly.
The water-soluble sodium carboxymethyl cellulose is natural cellulose which is nontoxic, nonirritating and nonimmunity-antigenicity-free to human bodies, and is an important cellulose derivative obtained by chemical improvement. Has excellent biocompatibility, and is widely used in medical products due to its excellent effect of preventing tissue adhesion.
Lysozyme, also known as muramidase, is an alkaline enzyme that hydrolyzes mucopolysaccharides in bacteria. Lysozyme dissolves bacteria by breaking the beta-1, 4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, breaking down the cell wall insoluble mucopolysaccharide into soluble glycopeptides, causing the contents of the broken cell wall to escape. It can be used as a natural anti-infective substance with antibacterial effect. Has antibacterial, antiviral, hemostatic, repercussive, analgesic, and tissue recovery promoting effects.
According to the invention, the lysozyme is added into the washing fluid component prepared from the sodium carboxymethylcellulose as the raw material, so that the effects of efficiently inhibiting bacteria and diminishing inflammation and promoting rapid healing of the wound are increased while the wound is cleaned and tissue adhesion is prevented, and a physical barrier can be finally formed to provide a favorable growth environment for wound healing. Through patent search, no report of adding lysozyme in biological polysaccharide flushing fluid is found at home and abroad.
Disclosure of Invention
The invention provides a biological polysaccharide flushing fluid with high-efficiency bacteriostasis and anti-inflammation and a preparation method thereof, which can clean wounds, prevent tissue adhesion, increase the effects of high-efficiency bacteriostasis and anti-inflammation on the wounds and promote rapid healing of the wounds, and finally form a physical barrier to provide a favorable growth environment for wound healing.
In order to achieve the above purpose of the present invention, the technical scheme adopted by the present invention is as follows:
the biological polysaccharide flushing fluid has the sodium carboxymethylcellulose substitution degree of 0.6-1.0. Preferably, the degree of substitution of sodium carboxymethylcellulose is 0.9.
The biological polysaccharide flushing fluid comprises, by weight, 0.5-6% of sodium carboxymethylcellulose, 0.3-5% of lysozyme, 0.1-0.9% of sodium chloride, 0.1-0.3% of a preservative, 0.01-0.1% of an acid-base regulator and the balance ultrapure water.
Preferably, the biological polysaccharide flushing fluid with high-efficiency bacteriostasis and anti-inflammation is prepared from 1.0% by weight of sodium carboxymethylcellulose, 3.0% by weight of lysozyme, 0.9% by weight of sodium chloride, 0.1% by weight of preservative and 0.04% by weight of acid-base regulator.
As a preferable mode of the invention, the biological polysaccharide flushing fluid is prepared by adding 1.0 percent of sodium carboxymethylcellulose, 3.0 percent of lysozyme, 0.9 percent of sodium chloride, 0.1 percent of preservative and 0.04 percent of acid-base regulator to 100 percent of ultrapure water in percentage by weight.
The preparation method of the biological polysaccharide flushing fluid with high-efficiency bacteriostasis and anti-inflammation comprises the following steps:
(1) dispersing sodium carboxymethylcellulose in purified water, and completely stirring to obtain a solution A;
(2) dispersing lysozyme in purified water, and fully stirring and dissolving to obtain a solution B;
(3) mixing the A, B solutions, and adding sodium chloride and antiseptic to obtain solution C;
(4) adding an acid-base regulator to regulate the pH value of the solution C to be between 5.5 and 7.5.
Sampling and measuring the performances such as pH value, viscosity and the like, and filling, sealing, packaging and sterilizing after the performances are qualified to obtain the biological polysaccharide flushing fluid with high efficiency, bacteriostasis and anti-inflammation.
In research and development, applicants found that in surgical operations, the wound surface or the surgical incision is treated by using physiological saline or a washing liquid prepared by mixing sodium carboxymethylcellulose as a raw material with traditional Chinese medicine components, chitosan or other saccharides in clinic. Compared with normal saline, the washing liquid has the effects of increasing adhesion resistance and the like, but provides a healing environment for the wound, and the components in the washing liquid have no outstanding effects of inhibiting bacteria, diminishing inflammation and promoting the wound to heal quickly. Further, the applicant finds that the carboxymethyl cellulose with the degree of substitution of 0.6-1.0 can improve the lysozyme activity and has a surprising synergistic effect. According to the invention, the lysozyme is added into the washing fluid component prepared from the sodium carboxymethylcellulose as the raw material, so that the effects of efficiently inhibiting bacteria and diminishing inflammation and promoting rapid healing of the wound are increased while the wound is cleaned and tissue adhesion is prevented, and a physical barrier can be finally formed to provide a favorable growth environment for wound healing.
Detailed Description
Example 1: the biological polysaccharide flushing fluid comprises, by weight, 2.0% of sodium carboxymethylcellulose, 0.5% of lysozyme, 0.9% of sodium chloride, 0.2% of a preservative, 0.05% of an acid-base regulator and the balance ultrapure water. The substitution degree of the sodium carboxymethyl cellulose is 0.7.
Sodium carboxymethylcellulose was dispersed in purified water and stirred completely to obtain solution a. And dispersing the lysozyme in purified water, and fully stirring and dissolving to obtain a solution B. A, B solution was mixed, and sodium chloride and preservative were added to obtain solution C. Adding an acid-base regulator to regulate the pH value of the solution C to be between 5.5 and 7.5. Sampling and measuring the performances such as pH value, viscosity and the like, and filling, sealing, packaging and sterilizing after the performances are qualified to obtain the biological polysaccharide flushing fluid with high efficiency, bacteriostasis and anti-inflammation.
Example 2: the biological polysaccharide flushing fluid comprises, by weight, 1.0% of sodium carboxymethylcellulose, 3% of lysozyme, 0.9% of sodium chloride, 0.1% of a preservative, 0.04% of an acid-base regulator and the balance ultrapure water. The substitution degree of the sodium carboxymethyl cellulose is 0.9.
Sodium carboxymethylcellulose was dispersed in purified water and stirred completely to obtain solution a. And dispersing the lysozyme in purified water, and fully stirring and dissolving to obtain a solution B. A, B solution was mixed, and sodium chloride and preservative were added to obtain solution C. Adding an acid-base regulator to regulate the pH value of the solution C to be between 5.5 and 7.5. Sampling and measuring the performances such as pH value, viscosity and the like, and filling, sealing, packaging and sterilizing after the performances are qualified to obtain the biological polysaccharide flushing fluid with high efficiency, bacteriostasis and anti-inflammation.
Control group 1: the anti-corrosion agent comprises, by weight, 1.0% of sodium carboxymethylcellulose, 0.9% of sodium chloride, 0.1% of a preservative, 0.04% of an acid-base regulator and the balance ultrapure water. The substitution degree of the sodium carboxymethyl cellulose is 0.9.
Sodium carboxymethylcellulose was dispersed in purified water and stirred completely to obtain solution a. Adding sodium chloride and preservative to obtain solution B. Adding an acid-base regulator to regulate the pH value of the solution B to be between 5.5 and 7.5. Sampling, measuring pH value, viscosity, etc., and bottling, sealing, packaging, and sterilizing.
The products of examples 1 and 2 and the control group, which was a biopolysaccharide rinse solution prepared by adding sodium carboxymethylcellulose alone, were tested for their bacteriostatic effects.
Claims (9)
1. A biological polysaccharide flushing fluid with high-efficiency bacteriostasis and anti-inflammation and a preparation method thereof are characterized in that: the anti-bacterial and anti-bacterial agent comprises, by weight, 0.5-6% of sodium carboxymethylcellulose, 0.3-5% of lysozyme, 0.1-0.9% of sodium chloride, 0.1-0.3% of preservative, 0.01-0.1% of acid-base regulator and the balance ultrapure water.
2. The efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and the preparation method thereof according to claim 1 are characterized in that: the substitution degree of the sodium carboxymethyl cellulose is 0.6-1.0.
3. The efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and the preparation method thereof according to claims 1-2, are characterized in that: the weight percentage of the sodium carboxymethylcellulose is 1.0%.
4. The efficient antibacterial and anti-inflammatory biopolysaccharide flushing fluid and the preparation method thereof according to claim 3, wherein the washing fluid comprises the following components: the weight percentage of the lysozyme is 3.0 percent.
5. The efficient antibacterial and anti-inflammatory biopolysaccharide flushing fluid and the preparation method thereof according to claim 4, wherein the washing fluid comprises the following components: the weight percentage of the sodium chloride is 0.9 percent.
6. The efficient antibacterial and anti-inflammatory biopolysaccharide flushing fluid and the preparation method thereof according to claim 5, wherein the washing fluid comprises the following components: the weight percentage of the preservative is 0.1%.
7. The efficient antibacterial and anti-inflammatory biopolysaccharide flushing fluid and the preparation method thereof according to claim 6, wherein the washing fluid comprises the following components: the weight percentage of the acid-base regulator is 0.04%.
8. The efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and the preparation method thereof according to claim 1 are characterized in that: the biological polysaccharide flushing fluid is prepared by adding 1.0 percent of sodium carboxymethylcellulose, 3.0 percent of lysozyme, 0.9 percent of sodium chloride, 0.1 percent of preservative and 0.04 percent of acid-base regulator to 100 percent of ultrapure water.
9. The preparation method of the biological polysaccharide flushing fluid with high antibacterial and anti-inflammatory effects as claimed in claims 1-8 is characterized by comprising the following steps:
(1) dispersing sodium carboxymethylcellulose in purified water, and completely stirring to obtain a solution A;
(2) dispersing lysozyme in purified water, and fully stirring and dissolving to obtain a solution B;
(3) mixing the A, B solutions, and adding sodium chloride and antiseptic to obtain solution C;
(4) adding an acid-base regulator to regulate the pH value of the solution C to be between 5.5 and 7.5;
sampling and measuring the performances such as pH value, viscosity and the like, and filling, sealing, packaging and sterilizing after the performances are qualified to obtain the biological polysaccharide flushing fluid with high efficiency, bacteriostasis and anti-inflammation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010526277.3A CN111760018A (en) | 2020-06-11 | 2020-06-11 | Efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and preparation method thereof |
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| CN202010526277.3A CN111760018A (en) | 2020-06-11 | 2020-06-11 | Efficient antibacterial and anti-inflammatory biological polysaccharide flushing fluid and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103203014A (en) * | 2013-03-06 | 2013-07-17 | 张巍 | Preparation method of blended functional surgical irrigating fluid |
| CN107753937A (en) * | 2016-08-19 | 2018-03-06 | 昆明倍健生物科技有限公司 | A kind of wound disinfection agent and preparation method thereof |
| CN108144048A (en) * | 2016-12-04 | 2018-06-12 | 青岛荣和天泽文化科技有限公司 | A kind of wound healing cleaning solution |
| CN110464735A (en) * | 2019-07-23 | 2019-11-19 | 海西纺织新材料工业技术晋江研究院 | A kind of sodium carboxymethylcellulose flushing liquor and preparation method thereof |
| US20200030385A1 (en) * | 2018-07-26 | 2020-01-30 | Huazhong Agricultural University | Water resistant enhanced wound healing film and preparation method thereof |
-
2020
- 2020-06-11 CN CN202010526277.3A patent/CN111760018A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103203014A (en) * | 2013-03-06 | 2013-07-17 | 张巍 | Preparation method of blended functional surgical irrigating fluid |
| CN107753937A (en) * | 2016-08-19 | 2018-03-06 | 昆明倍健生物科技有限公司 | A kind of wound disinfection agent and preparation method thereof |
| CN108144048A (en) * | 2016-12-04 | 2018-06-12 | 青岛荣和天泽文化科技有限公司 | A kind of wound healing cleaning solution |
| US20200030385A1 (en) * | 2018-07-26 | 2020-01-30 | Huazhong Agricultural University | Water resistant enhanced wound healing film and preparation method thereof |
| CN110464735A (en) * | 2019-07-23 | 2019-11-19 | 海西纺织新材料工业技术晋江研究院 | A kind of sodium carboxymethylcellulose flushing liquor and preparation method thereof |
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Application publication date: 20201013 |