CN111759528A - Portable cell storage syringe - Google Patents
Portable cell storage syringe Download PDFInfo
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- CN111759528A CN111759528A CN202010729607.9A CN202010729607A CN111759528A CN 111759528 A CN111759528 A CN 111759528A CN 202010729607 A CN202010729607 A CN 202010729607A CN 111759528 A CN111759528 A CN 111759528A
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- China
- Prior art keywords
- carbon dioxide
- cell storage
- injection tube
- constant temperature
- syringe
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- 238000003860 storage Methods 0.000 title claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 112
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 86
- 238000002347 injection Methods 0.000 claims abstract description 55
- 239000007924 injection Substances 0.000 claims abstract description 55
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 43
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 43
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 43
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 43
- 239000001301 oxygen Substances 0.000 claims abstract description 43
- 239000011229 interlayer Substances 0.000 claims abstract description 27
- 239000006285 cell suspension Substances 0.000 claims abstract description 26
- 239000002912 waste gas Substances 0.000 claims abstract description 13
- 239000007789 gas Substances 0.000 claims description 17
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 10
- 238000005192 partition Methods 0.000 claims description 9
- 210000000988 bone and bone Anatomy 0.000 claims description 8
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 56
- 238000002474 experimental method Methods 0.000 description 6
- 238000007789 sealing Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 208000006678 Abdominal Neoplasms Diseases 0.000 description 1
- 206010019695 Hepatic neoplasm Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 238000009580 stem-cell therapy Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
- A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
- A61J1/14—Details; Accessories therefor
- A61J1/20—Arrangements for transferring or mixing fluids, e.g. from vial to syringe
- A61J1/2096—Combination of a vial and a syringe for transferring or mixing their contents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/16—Disinfection, sterilisation or deodorisation of air using physical phenomena
- A61L9/18—Radiation
- A61L9/20—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
- A61M5/32—Needles; Details of needles pertaining to their connection with syringe or hub; Accessories for bringing the needle into, or holding the needle on, the body; Devices for protection of needles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/44—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests having means for cooling or heating the devices or media
- A61M5/445—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests having means for cooling or heating the devices or media the media being heated in the reservoir, e.g. warming bloodbags
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Heart & Thoracic Surgery (AREA)
- Anesthesiology (AREA)
- Vascular Medicine (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses a portable cell storage injector, which comprises an injector body, an oxygen and carbon dioxide supply device, a waste gas treatment device and a temperature control device, wherein the injector body comprises an injection tube for containing cell suspension and a piston positioned in the injection tube, and the piston is tightly matched with the inner wall of the injection tube; the injector body is provided with an air inlet communicated with the oxygen and carbon dioxide supply device and an air outlet communicated with the waste gas treatment device, the air inlet and the air outlet are communicated with the injection tube, the temperature control device comprises a hollow interlayer sleeved outside the injection tube and a constant temperature water tank filled with constant temperature water, and the hollow interlayer is provided with a water inlet and a water outlet communicated with the constant temperature water tank. So set up, this syringe is stored to portable cell collects and stores and injects as an organic whole, can guarantee to prolong cell storage time under aseptic condition, and is convenient for follow-up injection in to the animal body, strives for more animal operation preparation time for the researcher, provides convenience when guaranteeing the cell quality.
Description
Technical Field
The application relates to the technical field of cell storage, more specifically relates to a syringe is stored to portable cell.
Background
In medical research, some animal experiments, including modeling of abdominal tumors, liver or ovary tumors, bone in situ tumors, and stem cell therapy experiments, require injection of suspensions of relevant tumor cells, stem cells, or normal living cells into the animal body, so as to perform the corresponding experiments. The most essential requirement is that all cells must be viable and remain in a better cellular state.
However, the superclean bench for treating cells is usually located in a relatively sterile cell room, and the animal experiment operating platform is usually located in an animal room, so researchers often take the cells out of an incubator suitable for cell growth in the cell room, then treat the cells in the superclean bench to obtain cell suspension, and place the cell suspension in a closed sterile container tube, so that the cells are free from oxygen and carbon dioxide supply and are guaranteed to be constant in temperature, and therefore the researchers are required to inject the cell suspension into the animal body in a very short time.
However, in actual operation, it usually takes 30 minutes to 2 hours from obtaining the cell suspension to preparing the animal for surgery and injecting into the animal, and if an accident occurs, it takes longer time, so that the state of the cells in the cell suspension in the closed container tube will change, and the quality of the cells cannot be guaranteed, or even the cells die.
Therefore, how to solve the problem that the state of cells in a cell suspension in a closed container is easy to change and the quality of the cells cannot be guaranteed or the cells die due to long time consumption in the operation of animal experiments is a key technical problem to be solved by the technical personnel in the field.
Disclosure of Invention
In order to overcome the problems in the related art at least to a certain extent, the present application is directed to a portable cell storage syringe, which can solve the problem that the state of cells in a cell suspension in a closed container is easily changed to fail to ensure the quality of the cells or cause cell death in the operation of performing an animal experiment, which takes a long time.
The application provides a syringe is stored to portable cell, including:
the injection device comprises an injector body, a valve body and a valve body, wherein the injector body comprises an injection tube for containing cell suspension and a piston positioned in the injection tube, and the piston is tightly matched with the inner wall of the injection tube;
the injector body is provided with an air inlet communicated with the oxygen and carbon dioxide supply device and an air outlet communicated with the waste gas treatment device, the air inlet and the air outlet are both communicated with the injection pipe,
the temperature control device comprises a hollow interlayer sleeved outside the injection pipe and a constant temperature water tank filled with constant temperature water, wherein the hollow interlayer is provided with a water inlet and a water outlet which are communicated with the constant temperature water tank.
Preferably, the syringe body still includes the syringe needle, syringe needle detachably connects the bottom of syringe, just the syringe needle is ordinary thin needle or bone puncture needle.
Preferably, at least one limiting protrusion for limiting the downward movement of the piston is arranged on the inner wall of the injection tube, and the limiting protrusion is positioned below the air inlet hole and/or the air outlet hole.
Preferably, the temperature control device further comprises a temperature controller arranged on the constant-temperature water tank and a heating element arranged in the constant-temperature water tank, and the temperature controller is electrically connected with the heating element.
Preferably, the temperature control device further comprises a water pump arranged at a water outlet or a water inlet of the constant-temperature water tank.
Preferably, the oxygen and carbon dioxide supply device comprises an oxygen supply tank and a carbon dioxide supply tank, flow control valves are arranged at outlets of the oxygen supply tank and the carbon dioxide supply tank, fans are arranged in the oxygen supply tank and the carbon dioxide supply tank, and air outlets of the two fans face the outlets of the oxygen supply tank and the carbon dioxide supply tank respectively.
Preferably, the waste gas treatment device comprises a sterilization exhaust box and an ultraviolet lamp positioned in the sterilization exhaust box, the sterilization exhaust box is provided with an air inlet hole and an exhaust hole, the air inlet hole is communicated with the air outlet, and the ultraviolet lamp is positioned between the air inlet hole and the exhaust hole.
Preferably, a handle for holding is arranged outside the top end of the hollow interlayer.
Preferably, the waste gas treatment device further comprises a support, the support comprises an arc support and a support rod, the top end of the support rod is connected with the arc support, the bottom end of the support rod is rotatably connected to the outer wall of the waste gas treatment device, the inner diameter of the arc support is larger than or equal to the outer diameter of the hollow interlayer, the arc support is matched with the handle, so that the handle can be hung on the arc support, and the length of the support rod is larger than that of the injector body.
Preferably, outlets of the oxygen supply tank and the carbon dioxide supply tank are communicated with the gas inlet through a three-way pipeline.
The technical scheme provided by the application can comprise the following beneficial effects:
the application provides an injector is stored to portable cell, including the injector body, oxygen and carbon dioxide feeding device, exhaust treatment device and temperature control device, the injector body can be in aseptic cell room with the cell suspension direct suction to inside storage, thereby follow-up can direct injection, oxygen and carbon dioxide feeding device can provide oxygen and carbon dioxide for the cell, exhaust treatment device can handle the waste gas etc. that the cell produced, temperature control device can provide constant temperature environment for the cell, guarantee the activity of cell.
The injector body comprises an injection tube and a piston positioned in the injection tube, the cell suspension is positioned in the injection tube, and the piston is tightly matched with the inner wall of the injection tube to ensure that the injection tube is in a closed sterile environment, so that external bacteria and the like are prevented from entering the injection tube. Pushing the plunger enables the cell suspension to be expelled from the bottom end of the syringe tube while the injection is being performed. The injector body is provided with an air inlet and an air outlet, the air inlet is communicated with an oxygen and carbon dioxide supply device so that oxygen and carbon dioxide can enter the injector body, and the air outlet is communicated with a waste gas treatment device so that waste gas and the like generated by cells can enter the waste gas treatment device. The temperature control device comprises a hollow interlayer and a constant temperature water tank filled with constant temperature water, the hollow interlayer is sleeved outside the injection pipe and is provided with a water inlet and a water outlet, and the water inlet and the water outlet are communicated with the constant temperature water tank, so that the water in the constant temperature water tank can flow into the hollow interlayer and then flow back into the constant temperature water tank from the hollow interlayer, and the water circulation is realized.
So set up, this syringe is stored to portable cell collects and stores and injects as an organic whole, can guarantee to prolong cell storage time under aseptic condition, and is convenient for follow-up injection in to the animal body, strives for more animal operation preparation time for the researcher, provides convenience when guaranteeing the cell quality.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and together with the description, serve to explain the principles of the application.
Fig. 1 is a state diagram illustrating the use of the present portable cell storage syringe, according to some exemplary embodiments;
FIG. 2 is a perspective view of a thermostatic water tank shown in accordance with some exemplary embodiments;
FIG. 3 is a perspective view of an oxygen and carbon dioxide supply assembly shown in accordance with some exemplary embodiments;
FIG. 4 is a perspective view of an exhaust treatment device shown in accordance with certain exemplary embodiments;
FIG. 5 is a schematic view of a generic fine needle tip shown in accordance with certain exemplary embodiments;
fig. 6 is a schematic view of a bone piercing needle according to some exemplary embodiments.
In the figure:
1. an injection tube; 2. a piston; 3. a hollow interlayer; 4. an air inlet; 5. an air outlet; 6. a water inlet; 7. a water outlet; 8. a limiting bulge; 9. a constant temperature water tank; 10. an oxygen supply tank; 11. a carbon dioxide supply tank; 12. a sterilization exhaust box; 13. a handle; 14. an arc-shaped support; 15. a strut; 16. a three-way pipeline; 17. a temperature controller; 18. a water pump; 19. a partition plate; 20. a power switch; 21. a through hole; 22. a flow control valve; 23. a fan; 24. a power control switch; 25. a supply hole; 26. an air inlet; 27. an exhaust hole; 28. an ultraviolet lamp; 29. an ultraviolet lamp switch; 30. a power line.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus consistent with certain aspects of the present application, as detailed in the appended claims.
Referring to fig. 1 to 6, the present embodiment provides a portable cell storage syringe, including a syringe body, an oxygen and carbon dioxide supply device, an exhaust gas treatment device and a temperature control device, where the syringe body can directly suck cell suspension into an aseptic cell chamber for storage, so that the cell suspension can be directly injected later, the oxygen and carbon dioxide supply device can provide oxygen and carbon dioxide for cells, the exhaust gas treatment device can treat exhaust gas generated by the cells, and the temperature control device can provide a constant temperature environment for the cells, thereby ensuring the activity of the cells.
Wherein, the syringe body is including injection syringe 1 and the piston 2 that is located injection syringe 1, and cell suspension is located injection syringe 1 to piston 2 closely cooperates with the inner wall of injection syringe 1, is airtight sterile environment in order to guarantee injection syringe 1, thereby avoids external bacterium etc. to enter into injection syringe 1. When an injection is performed, pushing the piston 2 causes the cell suspension to be expelled from the bottom end of the syringe 1.
As shown in fig. 1, the injector body is provided with an air inlet 4 and an air outlet 5, the air inlet 4 is detachably connected with an oxygen and carbon dioxide supply device through a pipeline so that oxygen and carbon dioxide can enter the injector body, and the air outlet 5 is communicated with an exhaust gas treatment device so that exhaust gas and the like generated by cells can enter the exhaust gas treatment device.
The temperature control device comprises a hollow interlayer 3 and a constant temperature water tank 9 filled with constant temperature water, wherein the hollow interlayer 3 is sleeved outside the injection tube 1, namely a gap is formed between the hollow interlayer 3 and the outer wall of the injection tube 1. The hollow interlayer 3 is provided with a water inlet 6 and a water outlet 7, the water inlet 6 and the water outlet 7 are detachably connected with the constant-temperature water tank 9 through pipelines, so that water in the constant-temperature water tank 9 can flow into the hollow interlayer 3 and then flow back into the constant-temperature water tank 9 from the hollow interlayer 3, and water circulation is realized; that is, the water in the constant temperature water tank 9 can flow into the gap and then flow back into the constant temperature water tank 9 from the gap. Thus, the constant-temperature water outside the injection tube 1 can ensure that the cell suspension liquid in the injection tube 1 is in a constant-temperature state, is beneficial to the growth of cells, and can ensure the activity of the cells.
It should be noted that the air inlet 4 and the air outlet 5 penetrate through the hollow interlayer 3, the air inlet 4 and the air outlet 5 are not communicated with the hollow interlayer 3, the air inlet 4 and the air outlet 5 are arranged oppositely, and a sealing ring is arranged between the air inlet 4 and the air outlet and between the air outlet and the hollow interlayer 3, so as to avoid the water leakage phenomenon of the hollow interlayer 3.
And the water inlet 6 and the water outlet 7 of the hollow interlayer 3 are both arranged at the top end of the hollow interlayer 3 and are oppositely arranged, so that the hollow interlayer 3 can be filled with constant-temperature water, and the cell suspension in the injection tube 1 can be wrapped by the constant-temperature water.
So set up, this syringe is stored to portable cell collects and stores and injects as an organic whole, can guarantee to prolong cell storage time under aseptic condition, and is convenient for follow-up injection in to the animal body, strives for more animal operation preparation time for the researcher, provides convenience when guaranteeing the cell quality.
In the preferred scheme of this embodiment, the syringe body still includes the syringe needle, and the syringe needle detachably connects the bottom of syringe 1 to in the syringe needle of dismantlement, change, thereby be convenient for recycle, can change the syringe needle of different models or the syringe 1 of different capacity specifically as required. Here, as shown in fig. 5, the injection needle may be a general thin needle for cell injection of abdominal cavity or soft tissue, etc.; as shown in fig. 6, it may also be a bone penetrating needle for injection of cells into bone. The needle part diameter of the ordinary thin needle head is smaller than that of the bone puncture needle head, and the needle part length of the ordinary thin needle head is larger than that of the bone puncture needle head.
In order to prevent the bottom end of the piston 2 from protruding into the cell suspension and contaminating the cell suspension, at least one limiting protrusion 8 is provided on the inner wall of the injection tube 1 for limiting the downward movement of the piston 2.
As shown in FIG. 1, the limiting protrusion 8 is located below the air inlet 26 or the air outlet, so that after the cell suspension is sucked, an air flowing space is located above the limiting protrusion 8.
It should be noted that the limiting protrusion 8 and the injection tube 1 may be an integral structure. And the limiting bulge 8 can be an annular bulge, and the inner diameter of the limiting bulge 8 is smaller than the outer diameter of the piston 2, so that the piston 2 can be prevented from extending into the cell suspension. Of course, the limiting protrusion 8 includes a plurality of small protrusions located in the same plane, and the diameter of the inner ring of the circular ring surrounded by the plurality of small protrusions is smaller than the outer diameter of the piston 2.
In this embodiment, the temperature control device further includes a temperature controller 17 and a heating element, the heating element is disposed in the constant temperature water tank 9 to heat water in the constant temperature water tank 9, the temperature controller 17 is disposed on the constant temperature water tank 9, and the temperature controller 17 is electrically connected to the heating element to control the heating element, thereby ensuring constant temperature of water in the constant temperature water tank 9. The heating member may be a ceramic heating sheet provided at the bottom end of the constant-temperature water tank 9.
And the temperature control device also comprises a water pump 18, the water pump 18 can be arranged at the water outlet 7 of the constant temperature water tank 9, and also can be arranged at the water inlet 6 of the constant temperature water tank 9 to form water circulation, thereby ensuring that the temperature of the water flowing through the hollow interlayer 3 is constant. Here, a water pump 18 is provided at the water outlet 7 of the thermostatic water tank 9.
As shown in fig. 2, in order to prevent water flowing back to the constant temperature water tank 9 from the water outlet 7 of the constant temperature water tank 9 from being directly discharged from the water outlet 7 by the water pump 18, a partition plate 19 is disposed in the constant temperature water tank 9, the partition plate 19 is located between the water inlet 6 and the water outlet 7 of the constant temperature water tank 9, one end of the partition plate 19 is fixedly connected to a first side wall where the water inlet 6 and the water outlet 7 are located, upper and lower ends of the partition plate 19 are fixedly connected to an inner wall of the constant temperature water tank 9 to separate the water inlet 6 from the water outlet 7, a gap is left between the other end of the partition plate 19 and a second side wall to allow water to pass through, the second side wall corresponds to the first side wall, and specifically.
The constant temperature water tank 9 is also provided with a power switch 20, the power switch 20 is electrically connected with the temperature controller 17, the power switch 20 is connected with a power supply, and the on-off of the power switch 20 can control the power connection and the power disconnection of the temperature controller 17.
In order to facilitate replacement and discharge of water in the constant temperature water tank 9 or supplement of water to the constant temperature water tank 9, a through hole 21 is formed in the second side wall of the constant temperature water tank 9, the through hole 21 is communicated with a water containing space in the constant temperature water tank 9, a sealing plug is detachably arranged outside the through hole 21 and can seal the through hole 21, and therefore the water leakage phenomenon of the constant temperature water tank 9 is avoided when the temperature control device is in a working state.
In this embodiment, as shown in fig. 3, the oxygen and carbon dioxide supply device includes an oxygen supply box 10 and a carbon dioxide supply box 11, the oxygen supply box 10 contains oxygen, the carbon dioxide supply box 11 contains carbon dioxide, and the outlets of the oxygen supply box 10 and the carbon dioxide supply box 11 are provided with flow control valves 22 so as to control the supply amount of oxygen and carbon dioxide.
In order to facilitate the entry of oxygen and carbon dioxide into the injector body, fans 23 are provided in both the oxygen supply tank 10 and the carbon dioxide supply tank 11, and the outlets of the two fans 23 face the outlets of the oxygen supply tank 10 and the carbon dioxide supply tank 11, respectively.
The oxygen supply box 10 and the carbon dioxide supply box 11 are both provided with a power control switch 24, and the two power control switches 24 are respectively electrically connected with the two fans 23 to control the opening and closing of the two fans 23.
In some embodiments, as shown in fig. 1, the outlets of the oxygen supply tank 10 and the carbon dioxide supply tank 11 are connected to the gas inlet 4 of the injector body by a three-way pipe 16 to reduce the use of pipes and the space occupied by the device.
It should be noted that the oxygen supply tank 10 and the carbon dioxide supply tank 11 are both provided with supply holes 25, the supply holes 25 are located on the side wall opposite to the outlet, and sealing plugs are detachably arranged outside both the supply holes 25, and the sealing plugs can seal the supply holes 25 to prevent oxygen and carbon dioxide from leaking.
In this embodiment, as shown in fig. 4, the exhaust gas treatment device includes a sterilization exhaust box 12 and an ultraviolet lamp 28 disposed in the sterilization exhaust box 12, the sterilization exhaust box 12 is provided with an air inlet 26 and an air outlet 27, the air inlet 26 is communicated with the air outlet 5 of the injector body so that the exhaust gas generated by the cells can enter the sterilization exhaust box 12. The ultraviolet lamp 28 is disposed between the air inlet hole 26 and the air outlet hole 27 and is fixedly installed on the inner wall of the sterilizing exhaust box 12, so that the exhaust gas entering from the air inlet hole 26 can be discharged after being irradiated by the ultraviolet lamp 28. The ultraviolet light 28 emits ultraviolet light to kill cells, viruses, etc. carried in the exhaust gas. The exhaust hole 27 may be connected to an exhaust gas collecting device to discharge the sterilized exhaust gas into the exhaust gas collecting device.
An ultraviolet lamp switch 29 is arranged on the sterilization exhaust box 12, and the ultraviolet lamp switch 29 is electrically connected with the ultraviolet lamp 28 and used for controlling the on-off of the ultraviolet lamp 28.
The constant temperature water tank 9, the oxygen supply tank 10, the carbon dioxide supply tank 11 and the sterilization exhaust tank 12 can be integrated, and a power line 30 is arranged, namely, the power switch 20, the power control switch 24 and the ultraviolet lamp switch 29 are all connected with the power line 30; or may be separately arranged, and may be determined according to actual conditions.
In other embodiments, a handle 13 is provided outside the top end of the hollow compartment 3 to facilitate gripping by an operator. In particular, two handles 13 may be provided to cooperate with the syringe body for facilitating subsequent injection operations. The two handles 13 are located below the water inlet 6 and the water outlet 7, respectively, to avoid the handles 13 interfering with the water inlet and outlet.
This portable cell storage syringe is still including the support, and the support is connected with arc support 14 including arc support 14 and branch 15, the top of branch 15, and specifically, arc support 14 and branch 15 can be integrated into one piece. The bottom end of the strut 15 is rotatably connected to the exhaust gas treatment device so as to be accommodated. Of course, the bottom end of the supporting rod 15 may be connected to the constant temperature water tank 9, and here, it is not particularly limited.
The internal diameter of the arc-shaped support 14 is greater than or equal to the external diameter of the hollow partition 3, and the arc-shaped support 14 is matched with the handle 13, so that the handle 13 can be hung on the arc-shaped support 14, and the syringe body can be hung on the bracket.
Wherein the length of the holder is greater than the length of the syringe body, so that the syringe body is prevented from tilting after being placed on the holder, and the cell suspension is prevented from contacting the piston 2.
This syringe is stored to portable cell, it is convenient to use, the low price, economy, it is practical, application scope is wide, can dismantle, but disinfection by sterilization, can cyclic utilization, also can change the syringe needle of different models or the syringe of different capacity as required, can change the syringe needle of different grade type, in order to satisfy different puncture injection demands, and be convenient for the cell to store for a long time, inside is sterile environment, can adjust the regulation that carries out oxygen, carbon dioxide content and temperature according to the existence of different cells, in order to be suitable for the existence of different cells. The storage time of the cells is prolonged, and sufficient time is left for the preparation of researchers before injection, so that the cells are convenient to transport and carry; the cell suspension can be directly sucked into the injector body for storage in a sterile cell room, so that subsequent direct injection can be realized, the phenomenon that the injector is used again to suck cells from the cell suspension container under the condition that bacteria exist outside an animal room and the like is avoided, and the possibility of secondary pollution is effectively avoided.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
It is understood that the same or similar parts in the above embodiments may be mutually referred to, and the same or similar parts in other embodiments may be referred to for the content which is not described in detail in some embodiments.
Although embodiments of the present application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present application, and that variations, modifications, substitutions and alterations may be made to the above embodiments by those of ordinary skill in the art within the scope of the present application.
Claims (10)
1. A portable cell storage syringe, comprising:
the injection device comprises an injector body, wherein the injector body comprises an injection tube (1) for containing cell suspension and a piston (2) positioned in the injection tube (1), and the piston (2) is tightly matched with the inner wall of the injection tube (1);
the injector body is provided with an air inlet (4) communicated with the oxygen and carbon dioxide supply device and an air outlet (5) communicated with the waste gas treatment device, the air inlet (4) and the air outlet (5) are both communicated with the injection tube (1),
the temperature control device comprises a hollow interlayer (3) arranged outside the injection tube (1) and a constant temperature water tank (9) filled with constant temperature water, wherein the hollow interlayer (3) is provided with a water inlet (6) and a water outlet (7) communicated with the constant temperature water tank (9).
2. The portable cell storage syringe according to claim 1, wherein the syringe body further comprises an injection needle detachably connected to the bottom end of the syringe (1), and the injection needle is a common thin needle or a bone penetrating needle.
3. The portable cell storage syringe according to claim 1, wherein the inner wall of the syringe (1) is provided with at least one limiting protrusion (8) for limiting the downward movement of the piston (2), and the limiting protrusion (8) is located below the air inlet hole (26) and/or the air outlet hole.
4. The portable cell storage syringe according to claim 1, wherein the temperature control device further comprises a temperature controller (17) disposed on the constant temperature water tank (9) and a heating element disposed in the constant temperature water tank (9), and the temperature controller (17) is electrically connected to the heating element.
5. The portable cell storage syringe according to claim 4, wherein the temperature control device further comprises a water pump (18) disposed at the water outlet (7) or the water inlet (6) of the thermostatic water tank (9).
6. The portable cell storage syringe according to claim 1, wherein the oxygen and carbon dioxide supply device comprises an oxygen supply box (10) and a carbon dioxide supply box (11), the outlets of the oxygen supply box (10) and the carbon dioxide supply box (11) are provided with flow control valves (22), the oxygen supply box (10) and the carbon dioxide supply box (11) are provided with fans (23), and the air outlets of the two fans (23) face the outlets of the oxygen supply box (10) and the carbon dioxide supply box (11), respectively.
7. The portable cell storage syringe of claim 1, wherein the waste gas treatment device comprises a sterilization exhaust box (12) and an ultraviolet lamp (28) located in the sterilization exhaust box (12), the sterilization exhaust box (12) is provided with an air inlet hole (26) and an air outlet hole (27), the air inlet hole (26) is communicated with the air outlet (5), and the ultraviolet lamp (28) is located between the air inlet hole (26) and the air outlet hole (27).
8. The portable cell storage syringe according to claim 1, wherein a handle (13) for holding is provided outside the top end of the hollow partition (3).
9. The portable cell storage syringe according to claim 8, further comprising a support, wherein the support comprises an arc-shaped support (14) and a support rod (15) with the top end connected with the arc-shaped support (14), the bottom end of the support rod (15) is rotatably connected to the outer wall of the waste gas treatment device, the inner diameter of the arc-shaped support (14) is larger than or equal to the outer diameter of the hollow partition (3), the arc-shaped support (14) is matched with the handle (13) so that the handle (13) can be hung on the arc-shaped support (14), and the length of the support rod (15) is larger than that of the syringe body.
10. The portable cell storage syringe of claim 6, wherein the outlets of the oxygen supply tank (10) and the carbon dioxide supply tank (11) are in communication with the gas inlet (4) through a three-way pipe (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010729607.9A CN111759528A (en) | 2020-07-27 | 2020-07-27 | Portable cell storage syringe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010729607.9A CN111759528A (en) | 2020-07-27 | 2020-07-27 | Portable cell storage syringe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111759528A true CN111759528A (en) | 2020-10-13 |
Family
ID=72727356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010729607.9A Pending CN111759528A (en) | 2020-07-27 | 2020-07-27 | Portable cell storage syringe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111759528A (en) |
-
2020
- 2020-07-27 CN CN202010729607.9A patent/CN111759528A/en active Pending
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