CN111758501A - Respiration mouth structure, mycelium culture device and culture method of plasticizer-free mycelium - Google Patents
Respiration mouth structure, mycelium culture device and culture method of plasticizer-free mycelium Download PDFInfo
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- CN111758501A CN111758501A CN202010658141.8A CN202010658141A CN111758501A CN 111758501 A CN111758501 A CN 111758501A CN 202010658141 A CN202010658141 A CN 202010658141A CN 111758501 A CN111758501 A CN 111758501A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
- B01D46/56—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with multiple filtering elements, characterised by their mutual disposition
- B01D46/58—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with multiple filtering elements, characterised by their mutual disposition connected in parallel
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a breathing port structure, a hypha culture device and a hypha culture method, wherein the device comprises a first breathing structure body and a second breathing structure body assembled with the first breathing structure body; the first respiratory structure is provided with a first respiratory opening; the second breathing structure is configured into a structure with the size matched with one side of the first breathing structure facing the inside of the breathing opening structure, and the second breathing structure is formed with a second breathing opening; a filter assembly embedded between the first respiratory structure and the second respiratory structure; the breathing port structure is formed by combining the first breathing structure body and the second breathing structure body, the first breathing port and the second breathing port are designed on the first breathing structure body and the second breathing structure body, airflow conduction is realized through a plurality of air passages formed between the first breathing port and the second breathing port, meanwhile, the filtering component is embedded in the air passages, and filtration and purification of runoff gas are realized.
Description
Technical Field
The invention relates to the technical field of solid culture of large fungi hyphae, in particular to a respiration mouth structure, a hypha culture device with the respiration mouth structure and a plasticizer-free mycelium culture method using the hypha culture device.
Background
At present, the industrial large-scale production of mycelium culture is difficult to realize, the existing mycelium culture mostly adopts plastic bags or plastic bottles, the operation is complicated, and the obtained mycelium quantity is small; meanwhile, when the plastic bag or the plastic bottle is sterilized at high temperature and is cultured in time, the plastic components are possibly transferred into the fungus materials in a micro-scale manner, so that plastic pollution is caused, food unsafe factors are caused, and harm is caused to human bodies.
There is not a hypha culture apparatus that can satisfy industrialization large-scale production in the prior art, and simultaneously, the cultivation of hypha needs to ensure going on that growth in-process mycelium breathes, still needs to guarantee simultaneously that the device external bacteria can not pollute the hypha under non-airtight environment, consequently, it is the technological problem that this field needs to solve urgently to provide one kind both can guarantee that the hypha growth in-process breathes and go on, can also avoid the bacterial contamination hypha breathe mouthful structure and hypha culture apparatus.
Disclosure of Invention
The invention aims to provide a breathing opening structure which is novel in structure, can effectively filter air, can ensure hypha breathing and avoid external bacterial pollution, a hypha culture device with the breathing opening structure, and a plasticizer-free mycelium culture method using the hypha culture device.
In order to achieve the above purpose, the invention provides the following technical scheme:
the breathing opening structure of the invention mainly comprises:
a first respiratory structure;
a second respiratory structure assembled with the first respiratory structure;
the first respiratory structure is provided with a first respiratory opening;
the second breathing structure is configured into a structure with the size matched with one side of the first breathing structure facing the inside of the breathing opening structure, and a second breathing opening is formed in the second breathing structure;
an airway is formed between the first breathing port and the second breathing port;
this respiratory opening structure still includes:
a filter assembly embedded between the first and second respiratory structures;
the air which flows through the air passage passes through the filtering component, and the breathing port structure is communicated with the outside through the air passage and filters the flowing air through the filtering component;
the first breathing structure body comprises a body and an extension body which is formed at one end of the body and extends along the axial direction of the body.
Further, the first breathing structure body is formed into an embedded groove in the body and the extension body through axial extension of the body and the extension body;
the second breathing structure is embedded in the embedded groove to be assembled with the first breathing structure.
Further, the first breathing port is formed in the body of the first breathing structure;
the first breathing openings are divided into two groups, namely a plurality of outer ring breathing openings which are arranged at intervals and a plurality of inner ring breathing openings which are arranged at intervals;
the outer ring breathing port is formed on the outer ring of the inner ring breathing port;
the middle position part of the body is inwards sunken to form a first sunken part, and the middle position part of the first sunken part is outwards sunken to form a second sunken part;
the outer ring breathing port is formed between the first concave part and the body, and the inner ring breathing port is formed between the second concave part and the first concave part;
the first sunken part is provided with a plurality of connecting ribs which extend along the radial direction and are uniformly distributed along the circumferential direction;
the first concave part is connected with one end of the body through a connecting rib, and the second concave part is connected with the first concave part through the other end of the connecting rib;
it is a plurality of outer lane is breathed the mouth and is passed through the splice bar is cut apart, and is a plurality of the inner circle is breathed the mouth and is passed through the splice bar is cut apart.
Further, the filter assemblies are divided into two groups, namely a first filter element and a second filter element;
the first filter element is of a circular ring structure and is embedded into a space between the first concave part and the body;
the second filter element is of a circular structure and is embedded into the second concave part;
the gas circulating through the outer ring breathing hole is filtered by the first filter element;
the gas circulating through the inner ring breathing hole is filtered by the second filter element;
the first filter piece and the second filter piece are made of non-woven fabrics.
Further, the second breathing structure is embedded in the first breathing structure, and the second breathing structure contacts and presses the first filter element and the second filter element;
the second breathing structure is provided with a plurality of second breathing ports;
the plurality of second breathing holes are arranged along the circumferential direction of the second breathing structural body at intervals.
The invention discloses a hypha culture device, which comprises a box body and a box cover buckled at the upper end of the box body, wherein the box cover is provided with a breathing opening structure.
Further, the box body is of a quadrilateral box body structure, a culture cavity is formed in the box body, and culture media are filled in the culture cavity;
the box body and the box cover are both made of stainless steel materials, and the plate thicknesses of the box body and the box cover are 0.5-1.0 mm;
the joint of the box body and the box cover is provided with a sealing gasket fixed on the box body and/or the box cover;
the sealing gasket is a silica gel gasket.
Furthermore, the box cover is provided with two groups of breathing port structures;
two mounting holes are formed in the box cover;
the breathing opening structure is arranged in the mounting hole.
Furthermore, an internal thread is formed in the mounting hole of the box cover;
an external thread is formed on the surface of an extending body of the first breathing structure body of the breathing opening structure;
the breathing port structure is connected in the mounting hole in a threaded manner;
the section of the breathing opening structure is circular, and the maximum outer diameter of the breathing opening structure is 60-80 mm.
The invention also discloses a culture method of the plasticizer-free mycelium, which mainly comprises the following steps:
s101, assembling a breathing port structure, namely separating a first breathing structure body and a second breathing structure body, checking the first breathing port and the second breathing port, respectively embedding a first filter piece and a second filter piece at specified positions, compacting, and embedding the second breathing structure body into the first breathing structure body to complete the assembling of the breathing port structure;
s102, processing a culture medium, namely processing the culture medium according to the type of the mycelium to be cultured, carrying out high-temperature sterilization on the culture medium, and placing the processed culture medium in a box body through a mounting hole of a box cover after the culture medium is cooled;
s103, mounting the breathing hole structure in the mounting hole to seal the mounting hole of the box cover;
and S104, placing the hypha culture device with the built-in culture medium in an environment with the humidity and the temperature meeting the culture requirements.
In the technical scheme, the breathing opening structure, the mycelium culture device and the culture method of the plasticizer-free mycelium have the following beneficial effects:
the breathing port structure is formed by combining the first breathing structure body and the second breathing structure body, the first breathing port and the second breathing port are designed on the first breathing structure body and the second breathing structure body, airflow conduction is realized through a plurality of air passages formed between the first breathing port and the second breathing port, meanwhile, the filtering component is embedded in the air passages, and filtration and purification of runoff gas are realized.
The invention also discloses a hypha culture device with the breathing opening structure, which utilizes the breathing opening structure to meet the breathing of the mycelium during growth, and can also utilize the filter assembly clamped in the middle to filter the flowing gas so as to prevent the external bacteria from polluting the mycelium in the box body.
Meanwhile, the invention discloses a culture method for culturing mycelia by using the mycelium culture device, in order to ensure that pollution is reduced, the mounting hole assembled with the breathing port structure can be used as a picking and placing channel of a culture medium, and meanwhile, the breathing and gas filtration during the growth of the mycelia are realized by using the breathing port structure, so that the culture environment is ensured to be in a sterile environment.
The mycelium culture device can meet the requirement of industrial large-scale production, has good culture effect, does not have plastic component pollution, and has high effective cost content in the cultured mycelium.
Drawings
In order to more clearly illustrate the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.
Fig. 1 is a schematic structural diagram of a breathing opening structure according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view of a first respiratory structure of a respiratory opening arrangement according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a second respiratory structure of a respiratory opening structure according to an embodiment of the present invention;
fig. 4 is a sectional view of a first respiratory structure and a second respiratory structure of a respiratory opening structure according to an embodiment of the present invention;
FIG. 5 is a sectional view of the first respiratory structure, the second respiratory structure and the filter assembly of the respiratory opening structure in an assembled state according to an embodiment of the present invention;
FIG. 6 is a schematic structural view of a hypha culture apparatus according to a second embodiment of the present invention;
FIG. 7 is a schematic view showing a connection structure between a case and a case cover of a hypha culture apparatus according to a second embodiment of the present invention.
Description of reference numerals:
1. a first respiratory structure; 2. a second respiratory structure; 3. a first recess; 4. a second recess; 5. an airway;
101. a body; 102. an extension body; 103. connecting ribs;
201. a second breathing port;
301. a breathing hole is arranged on the outer ring;
401. an inner ring breathing port;
601. a first filter member; 602. a second filter member;
701. a box body; 702. a box cover; 703. mounting holes; 704. and a gasket.
Detailed Description
In order to make the technical solutions of the present invention better understood, those skilled in the art will now describe the present invention in further detail with reference to the accompanying drawings.
The first embodiment is as follows:
as shown in fig. 1 to 5;
the breathing opening structure of the invention mainly comprises:
a first respiratory structure 1;
a second respiratory structure 2 assembled with the first respiratory structure 1;
the first respiratory structure 1 is formed with a first respiratory opening;
the second breathing structure 2 is configured into a structure with the size matched with one side of the first breathing structure 1 facing the inside of the breathing opening structure, and the second breathing structure 2 is provided with a second breathing opening 201;
an airway 5 is formed between the first breathing port and the second breathing port 201;
this respiratory opening structure still includes:
a filter assembly embedded between the first 1 and second 2 respiratory structures;
the air which flows through the air passage 5 passes through the filtering component, the breathing port structure is communicated with the outside through the air passage 5, and the air which flows through the filtering component is filtered;
the first respiratory structure 1 includes a body 1101, and an extension 102 formed at one end of the body 101 and extending axially along the body 101.
Specifically, this embodiment one discloses a mouth structure of breathing, and this mouth structure of breathing need can guarantee gaseous smooth circulation to can let the gaseous filtration that can pass through filtering component of circulation. The breathing opening structure specifically comprises a first breathing structure body 1 and a second breathing structure body 2, wherein the first breathing structure body 1 and the second breathing structure body 2 are assembled in a scarf joint mode. When the respirator is manufactured, a first breathing opening is formed in the first breathing structure 1, a second breathing opening 201 is formed in the second breathing structure 2, an air passage 5 is formed between the first breathing opening and the second breathing opening 201, and air flow at two ends of the breathing opening structure forms air flow and air exchange through the air passage 5.
Preferably, the first breathing structure 1 in the first embodiment is formed as an embedded groove in the body 101 and the extension body 102 by axial extension;
the second respiratory structure 2 is fitted into the fitting groove to be assembled with the first respiratory structure 1.
First, since the first respiratory structure 1 and the second respiratory structure 2 are used in combination, it is necessary to consider the problem of mounting the second respiratory structure 2, and the first respiratory structure 1 in this embodiment includes a main body 101 and an extension body 102, and due to the extension action of the extension body 102 along the axial direction thereof, an inwardly recessed insertion groove is formed on one side of the first respiratory structure 1, and during processing, the size and shape of the second respiratory structure 2 are designed so that the second respiratory structure 2 can be fitted into the insertion groove and can be formed into an interference fit or a clearance fit as much as possible, thereby realizing the assembly of the two.
Preferably, in the present embodiment, the first breathing opening is formed in the body 101 of the first breathing structure 1;
the first breathing openings are divided into two groups, namely a plurality of outer ring breathing openings 301 which are arranged at intervals and a plurality of inner ring breathing openings 401 which are arranged at intervals;
the outer ring breathing hole 301 is formed on the outer ring of the inner ring breathing hole 401;
the middle part of the body 101 is formed into a first concave part 3 by being concave inwards, and the middle part of the first concave part 3 is formed into a second concave part 4 by being concave outwards;
an outer ring breathing port 301 is formed between the first concave part 3 and the body 100, and an inner ring breathing port 401 is formed between the second concave part 4 and the first concave part 3;
the first recessed part 3 is provided with a plurality of connecting ribs 103 which extend along the radial direction and are uniformly distributed along the circumferential direction;
the first concave part 3 is connected with one end of the body 101 through a connecting rib 103, and the second concave part 4 is connected with the first concave part 3 through the other end of the connecting rib 103;
the plurality of outer-ring breathing openings 301 are divided by the connecting ribs 103, and the plurality of inner-ring breathing openings 401 are divided by the connecting ribs 103.
The structure of the first breathing structure 1 is further defined, and a first breathing opening is processed on the first breathing structure 1, and the first breathing opening has two parts, one part is the outer circle breathing opening 301, and the other part is the inner circle breathing opening 401; the first concave part 3 and the second concave part 4 are formed at the designated position of the first breathing structure body 1 in a concave/convex manner towards two directions, and because the first concave part 3 and the second concave part 4 are formed in a concave/convex manner towards the designated direction, a certain height difference exists between the first concave part and the adjacent structure, and the outer circle breathing hole 301 and the inner circle breathing hole 401 are respectively and correspondingly arranged at the connecting position with the height difference, so that the assembled filter assembly can be conveniently embedded into the connecting position and can filter the gas flowing through the connecting position. In addition, the first embodiment specifically defines the main connection structure of the first concave portion 3 and the second concave portion 4 after the concave/convex, which is realized by the connection rib 103 to connect the three. Meanwhile, the breathing opening is divided by the connecting ribs 103, so that the airflow can circulate from any direction.
Preferably, the filter assemblies in this embodiment are divided into two groups, namely a first filter 601 and a second filter 602;
the first filter 601 is of a circular ring structure, and the first filter 601 is embedded in a space between the first concave part 3 and the body 101;
the second filter member 602 has a circular structure, and the second filter member 602 is embedded in the second recess 4;
the gas flowing through the outer ring breathing hole 301 is filtered by the first filter element 601;
the gas flowing through the inner circle breathing hole 401 is filtered by the second filter element 602;
the first filter 601 and the second filter 602 are made of non-woven fabric.
The non-woven fabrics is common filter material, and convenient for material selection, and the non-woven fabrics is adopted as filtering piece in this embodiment, of course, as the technical scheme of expansibility, to different filtration requirements, can also select for use other kinds of filter material. And will not be described in detail here.
Preferably, in this embodiment, the second respiratory structure 2 is embedded in the first respiratory structure 1, and the second respiratory structure 2 contacts and presses the first filter 601 and the second filter 602;
the second breathing structure 2 is provided with a plurality of second breathing holes 201;
the plurality of second breathing holes 201 are arranged at intervals along the circumferential direction of the second breathing structure 2.
After the first filter 601 and the second filter 602 are respectively inserted into the designated positions, the second breathing structure 2 is used to press and hold the two, and a complete breathing opening structure is formed by combination.
Example two:
as shown in fig. 1 to 5, and fig. 6 to 7;
the invention discloses a hypha culture device, which comprises a box body 701 and a box cover 702 buckled at the upper end of the box body 701, wherein the box cover 702 is provided with a breathing port structure.
The box body 701 is of a quadrilateral box body structure, a culture cavity is formed in the box body 701, and a culture medium is filled in the culture cavity;
the box body 701 and the box cover 702 are both made of stainless steel, and the plate thickness of the box body 701 and the box cover 702 is 0.5-1.0 mm;
the joint of the box body 701 and the box cover 702 is provided with a gasket 704 fixed on the box body 701 and/or the box cover 702;
the gasket 704 is a silicone gasket.
In order to avoid the technical problem that the culture medium and the mycelium are polluted by plastics when plastic bags or plastic bottles are used as culture devices in the prior art, the box body 701 and the box cover 702 are made of stainless steel materials, the sealing gasket 704 is arranged at the joint of the box body 701 and the box cover 702 to avoid poor sealing performance of the joint of the box body 701 and the box cover 702, the box body 701 and the box cover 702 can be ensured to be stably installed through elastic deformation of the sealing gasket 704, the sealing is realized, and the stability of a packaging structure is ensured.
Further: the case cover 702 is provided with two sets of breathing hole structures;
two mounting holes 703 are formed in the box cover 702;
the breathing port structure is mounted in the mounting hole 703.
Wherein, the mounting hole 703 of the box cover 702 is internally provided with an internal thread;
the surface of the extending body of the first respiratory structure body 1 of the respiratory opening structure is provided with an external thread;
the breathing port structure is connected in the mounting hole 703 through threads;
the section of the breathing opening structure is circular, and the maximum outer diameter of the breathing opening structure is 60-80 mm.
Example three:
the invention also discloses a culture method of the plasticizer-free mycelium, which mainly comprises the following steps:
s101, assembling a breathing hole structure, namely separating a first breathing structure body 1 and a second breathing structure body 2, checking the first breathing hole and the second breathing hole 201, respectively embedding a first filter member 601 and a second filter member 602 at specified positions, compacting, and embedding the second breathing structure body 2 into the first breathing structure body 1 to complete the assembling of the breathing hole structure;
s102, processing a culture medium, namely processing the culture medium according to the type of the mycelium to be cultured, carrying out high-temperature sterilization on the culture medium, and placing the processed culture medium in a box body 701 from an installation hole 703 of a box cover 702 after the culture medium is cooled;
s103, mounting the breathing hole structure in the mounting hole 703 to close the mounting hole 703 of the box cover 702;
and S104, placing the hypha culture device with the built-in culture medium in an environment with the humidity and the temperature meeting the culture requirements.
In the technical scheme, the breathing opening structure, the mycelium culture device and the mycelium culture method provided by the invention have the following beneficial effects:
the breathing port structure is formed by combining the first breathing structure body 1 and the second breathing structure body 2, the first breathing port 201 and the second breathing port 201 are designed on the first breathing structure body and the second breathing structure body, airflow conduction is realized through a plurality of air passages 5 formed between the first breathing port 201 and the second breathing port 201, meanwhile, a filtering component is embedded into the air passages 5, and radial flow gas filtering and purifying are realized.
The invention also discloses a hypha culture device with the breathing port structure, which utilizes the breathing port structure to meet the breathing of the mycelium during growth, and can also utilize the filter assembly clamped in the middle to filter the flowing gas so as to prevent the external bacteria from polluting the mycelium in the box body 701.
Meanwhile, the invention discloses a culture method for culturing mycelia by using the mycelium culture device, in order to ensure that pollution is reduced, the mounting hole 703 assembled with the breathing port structure can be used as a picking and placing channel of a culture medium, and meanwhile, the breathing and gas filtration during the growth of the mycelia are realized by using the breathing port structure, so that the culture environment is ensured to be in a sterile environment.
The mycelium culture device can meet the requirement of industrial large-scale production, has good culture effect, does not have plastic component pollution, and has high effective cost content in the cultured mycelium.
While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.
Claims (10)
1. Respiratory opening structure, its characterized in that, this structure mainly includes:
a first respiratory structure (1);
a second respiratory structure (2) assembled with the first respiratory structure (1);
the first respiratory structure (1) is provided with a first respiratory opening;
the second breathing structure (2) is configured into a structure with the size matched with one side of the first breathing structure (1) facing the inner part of the breathing opening structure, and the second breathing structure (2) is provided with a second breathing opening (201);
an air passage (5) is formed between the first breathing port and the second breathing port (201);
this respiratory opening structure still includes:
a filter assembly interposed between said first (1) and second (2) respiratory structures;
the gas which flows through the air passage passes through the filtering component, and the breathing port structure is communicated with the outside through the air passage (5) and filters the flowing gas through the filtering component;
the first respiratory structure body (1) comprises a body (101) and an extension body (102) which is formed at one end of the body (101) and extends along the axial direction of the body (101).
2. The breathing port structure according to claim 1, wherein the first breathing structure (1) is formed inside it as an embedded groove by the axial extension of the body (101) and extension body (102);
the second breathing structure body (2) is embedded in the embedded groove to be assembled with the first breathing structure body (1).
3. The breathing opening arrangement according to claim 2, wherein the first breathing opening is formed in a body (101) of the first breathing structure (1);
the first breathing openings are divided into two groups, namely a plurality of outer ring breathing openings (301) which are arranged at intervals and a plurality of inner ring breathing openings (401) which are arranged at intervals;
the outer ring breathing port (301) is formed on the outer ring of the inner ring breathing port (401);
the middle position part of the body (101) is inwards sunken to form a first sunken part (3), and the middle position part of the first sunken part (3) is outwards sunken to form a second sunken part (4);
the outer ring breathing port (301) is formed between the first concave part (3) and the body (1), and the inner ring breathing port (401) is formed between the second concave part (4) and the first concave part (3);
the first concave part (3) is provided with a plurality of connecting ribs (103) which extend along the radial direction and are uniformly distributed along the circumferential direction;
the first concave part (3) is connected with one end of the body (1) through a connecting rib (103), and the second concave part (4) is connected with the first concave part (3) through the other end of the connecting rib (103);
the outer ring breathing openings (301) are divided by the connecting ribs (103), and the inner ring breathing openings (401) are divided by the connecting ribs (103).
4. The respiratory opening structure of claim 3 wherein the filter assembly is divided into two groups, a first filter (601) and a second filter (602);
the first filter piece (601) is of a circular ring structure, and the first filter piece (601) is embedded into a space between the first concave part (3) and the body (101);
the second filter element (602) is of a circular structure, and the second filter element (602) is embedded in the second concave part (4);
the gas circulating through the outer ring breathing hole (301) is filtered by the first filter element (601);
the gas circulating through the inner circle breathing hole (401) is filtered by the second filter element (602);
the first filter piece (601) and the second filter piece (602) are both made of non-woven fabrics.
5. The respiratory orifice structure of claim 4, wherein the second respiratory structure (2) is embedded in the first respiratory structure (1) and the second respiratory structure (2) contacts and presses against the first filter element (601) and the second filter element (602);
the second breathing structure (2) is provided with a plurality of second breathing holes (201);
the plurality of second breathing holes (201) are arranged at intervals along the circumferential direction of the second breathing structural body (2).
6. A hypha culture device, this hypha culture device includes box (701), and lock in the case lid (702) of box (701) upper end, characterized in that, install the breathing opening structure of any claim 1 to 5 on the case lid (702).
7. The mycelium culture device according to claim 6, wherein the box body (701) has a quadrangular box structure, and the box body (701) has a culture chamber therein, and the culture chamber is filled with a culture medium;
the box body (701) and the box cover (702) are both made of stainless steel, and the plate thickness of the box body (701) and the plate thickness of the box cover (702) are 0.5-1.0 mm;
the joint of the box body (701) and the box cover (702) is provided with a sealing gasket (704) fixed on the box body (701) and/or the box cover (702);
the sealing pad (704) is a silica gel pad.
8. The hypha cultivation device according to claim 7, wherein the box cover (702) is mounted with two sets of the breathing orifice structures;
two mounting holes (703) are formed in the box cover (702);
the breathing opening structure is mounted in the mounting hole (703).
9. The hypha culture apparatus according to claim 8, wherein an internal thread is formed in the mounting hole of the case cover (702);
the surface of an extending body (102) of a first breathing structure body (1) of the breathing opening structure is provided with an external thread;
the breathing port structure is connected in the mounting hole (703) in a threaded manner;
the section of the breathing opening structure is circular, and the maximum outer diameter of the breathing opening structure is 60-80 mm.
10. The culture method of the mycelium without the plasticizer is characterized by mainly comprising the following steps:
s101, assembling a breathing port structure, namely separating a first breathing structure body (1) and a second breathing structure body (2), checking the first breathing port and the second breathing port (201), respectively embedding a first filter piece (601) and a second filter piece (602) at specified positions, compacting, and embedding the second breathing structure body (2) into the first breathing structure body (1) to complete the assembling of the breathing port structure;
s102, processing a culture medium, namely processing the culture medium according to the type of the mycelium to be cultured, carrying out high-temperature sterilization on the culture medium, and placing the processed culture medium in a box body (701) from a mounting hole of a box cover (702) after the culture medium is cooled;
s103, mounting the breathing hole structure in the mounting hole (703) to close the mounting hole (703) of the box cover (702);
and S104, placing the hypha culture device with the built-in culture medium in an environment with the humidity and the temperature meeting the culture requirements.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010658141.8A CN111758501B (en) | 2020-07-09 | 2020-07-09 | Respiration mouth structure, mycelium culture device and plasticizer-free mycelium culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010658141.8A CN111758501B (en) | 2020-07-09 | 2020-07-09 | Respiration mouth structure, mycelium culture device and plasticizer-free mycelium culture method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111758501A true CN111758501A (en) | 2020-10-13 |
| CN111758501B CN111758501B (en) | 2022-11-11 |
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| CN202010658141.8A Active CN111758501B (en) | 2020-07-09 | 2020-07-09 | Respiration mouth structure, mycelium culture device and plasticizer-free mycelium culture method |
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| CN111758501B (en) | 2022-11-11 |
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