CN111744057A - Preparation method of integrally-formed magnetic induction nerve conduit - Google Patents

Preparation method of integrally-formed magnetic induction nerve conduit Download PDF

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CN111744057A
CN111744057A CN202010489942.6A CN202010489942A CN111744057A CN 111744057 A CN111744057 A CN 111744057A CN 202010489942 A CN202010489942 A CN 202010489942A CN 111744057 A CN111744057 A CN 111744057A
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nerve
layer
pipeline
growth factor
pdms
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CN111744057B (en
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刘鐘阳
唐佩福
张阳
张里程
崔翔
刘建恒
李明
李建涛
邓俊豪
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Chinese PLA General Hospital
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Abstract

The invention discloses a preparation method of an integrally formed magnetic induction nerve conduit, which comprises the following steps: preparing a pipeline by using a PDMS (polydimethylsiloxane) mould, placing a cylindrical mould in the pipeline, and keeping a gap between the outer side wall of the cylindrical mould and the inner side wall of the pipeline, respectively injecting a magnetic nanoparticle layer and a nerve growth factor layer into the gap by using a microfluidic method, freeze-drying the magnetic nanoparticle layer and the nerve growth factor layer, extracting the cylindrical mould from an inner cavity of the nerve growth factor layer after freeze-drying, injecting a nerve stem cell layer along the inner cavity side wall of the nerve growth factor layer by using the microfluidic method, and removing the PDMS mould to obtain the nerve conduit. The nerve conduit prepared by the method simultaneously has magnetic induction particles, nerve growth factors and nerve stem cells, and can well promote the repair of nerve defects.

Description

Preparation method of integrally-formed magnetic induction nerve conduit
Technical Field
The invention relates to the field of medical instruments, in particular to a nerve conduit for repairing nerve defects.
Background
The treatment of nerve defects caused by serious peripheral nerve injury is always a clinical problem. The treatment currently varies according to the distance of the defect. When the defect distance is short, the broken end suturing method is often adopted directly. When the defect distance is longer, alternative treatment is required. At present, autologous nerve transplantation is the "gold standard", but has the problems of donor site damage, functional impairment and the like. The method adopting the tissue engineering nerve conduit has greatly progressed in recent years, and partial research shows that the treatment effect of the nerve conduit can approach autologous nerve transplantation. Studies have shown that the following three methods are helpful in the repair of neurological defects: (1) the magnetic induction particles are added into the nerve conduit to play a magnetic induction effect, so that the nerve repair and regeneration promoting effect of the nerve conduit can be effectively promoted; (2) factors such as Nerve Growth Factor (NGF) can effectively promote the repair of peripheral nerve injury; (3) the nerve stem cells are added into the nerve conduit to promote the repair of peripheral nerve injury. However, the conventional nerve conduit cannot combine the above three methods.
Disclosure of Invention
The invention aims to solve the technical problem of providing a preparation method of an integrally formed magnetic induction nerve conduit, wherein the nerve conduit prepared by the method simultaneously has magnetic induction particles, nerve growth factors and nerve stem cells, and can well promote the repair of nerve defects.
The invention discloses a preparation method of an integrally formed magnetic induction nerve conduit, which comprises the following steps:
a pipe is prepared by using the PDMS mold,
placing a cylindrical mold in the pipeline, and leaving a gap between the outer side wall of the cylindrical mold and the inner side wall of the pipeline,
respectively injecting a magnetic nanoparticle layer and a nerve growth factor layer into the gap by a microfluidic method, wherein the magnetic nanoparticle layer is injected along the inner side wall of the pipeline, the nerve growth factor layer is injected along the outer side wall of the cylindrical mold,
freeze-drying the magnetic nanoparticle layer and the nerve growth factor layer, extracting the cylindrical mold from the inner cavity of the nerve growth factor layer after freeze-drying,
injecting a neural stem cell layer along the inner cavity side wall of the nerve growth factor layer by using a microfluidic method,
and (4) removing the PDMS mold to obtain the nerve conduit.
The invention relates to a preparation method of an integrally formed magnetic induction nerve conduit, wherein a magnetic nanoparticle layer comprises magnetic nanoparticles and a material coated outside the magnetic nanoparticles, and the material coated outside the magnetic nanoparticles is at least one of PLGA, chitosan and GelMA.
The invention discloses a preparation method of an integrated magnetic induction nerve conduit, wherein a nerve growth factor layer is a solution containing nerve growth factors, BSA (bovine serum albumin) and a water-soluble material, the nerve growth factors are at least one of HIF-1 alpha and rapamycin, and the water-soluble material is at least one of PVA (polyvinyl acetate), silk fibroin and type I collagen.
The invention discloses a preparation method of an integrated forming magnetic induction nerve conduit, wherein a nerve stem cell layer is hydrogel containing nerve stem cells, and the hydrogel is at least one of PEG, gelatin and collagen.
The invention discloses a preparation method of an integrally-molded magnetic induction nerve conduit, wherein when a pipeline is prepared by using a PDMS mold, two PDMS templates are adopted, a chemical etching method is used for manufacturing a strip-shaped groove on each PDMS template, then the two PDMS templates are subjected to oxygen beating in a plasma cleaning machine for 30 seconds to destroy the silicon-oxygen bond of the PDMS templates, then the two PDMS templates are tightly bonded under the condition that the two strip-shaped grooves correspond to each other to obtain the PDMS mold, and the pipeline is composed of the two strip-shaped grooves.
The invention discloses a preparation method of an integrated forming magnetic induction nerve conduit, wherein when a cylindrical mold is placed in a pipeline, the cylindrical mold and the pipeline are coaxially arranged.
The preparation method of the integrally formed magnetic induction nerve conduit is different from the prior art in that the nerve conduit is manufactured by a micro-fluidic control method and comprises an outer layer, a middle layer and an inner layer, wherein the outer layer is a magnetic nanoparticle layer, the middle layer is a nerve growth factor layer, the inner layer is a nerve stem cell layer, and the magnetic nanoparticles, the nerve growth factor and the nerve stem cells in the nerve conduit can promote the repair of nerve defects, so the effect is better.
The invention will be further explained with reference to the drawings.
Drawings
FIG. 1 is a schematic view of a method for manufacturing an integrally formed magnetically inductive nerve conduit according to the present invention;
fig. 2 is a schematic cross-sectional view of a nerve conduit prepared using the present invention.
Detailed Description
As shown in fig. 1, the method for preparing the integrally formed magnetic induction nerve conduit of the present invention comprises the following steps:
a pipe is prepared using the PDMS mold 4,
placing a cylindrical mold in the pipeline, and leaving a gap between the outer side wall of the cylindrical mold and the inner side wall of the pipeline,
respectively injecting a magnetic nanoparticle layer 5 and a nerve growth factor layer 6 into the gap by using a microfluidic method, wherein the magnetic nanoparticle layer 5 is injected along the inner side wall of the pipeline, the nerve growth factor layer 6 is injected along the outer side wall of the cylindrical mould,
freeze-drying the magnetic nanoparticle layer 5 and the nerve growth factor layer 6, extracting the cylindrical mold from the inner cavity of the nerve growth factor layer 6 after freeze-drying,
injecting the neural stem cell layer 7 along the inner cavity side wall of the nerve growth factor layer 6 by using a microfluidic method,
and (4) removing the PDMS mold 4 to obtain the nerve conduit.
The invention discloses a preparation method of an integrally formed magnetic induction nerve conduit, wherein a magnetic nanoparticle layer 5 comprises magnetic nanoparticles 501 and a material coated outside the magnetic nanoparticles 501, and the material coated outside the magnetic nanoparticles 501 is at least one of PLGA, chitosan and GelMA.
The invention discloses a preparation method of an integrated magnetic induction nerve conduit, wherein a nerve growth factor layer 6 is a solution containing nerve growth factors, BSA (bovine serum albumin) and a water-soluble material, the nerve growth factors are at least one of HIF-1 alpha and rapamycin, and the water-soluble material is at least one of PVA (polyvinyl acetate), silk fibroin and type I collagen.
The invention discloses a preparation method of an integrated forming magnetic induction nerve conduit, wherein a nerve stem cell layer 7 is hydrogel containing nerve stem cells, and the hydrogel is at least one of PEG, gelatin and collagen.
As shown in fig. 1 in conjunction with fig. 2, the magnetic nanoparticle layer 5 is injected into the gap between the pipe and the cylindrical mold using the first injector 1, and the magnetic nanoparticle layer 5 becomes the outer layer of the nerve conduit since the magnetic nanoparticle layer 5 is injected along the inner sidewall of the pipe; injecting the nerve growth factor layer 6 into the gap between the pipeline and the cylindrical mold by using the second injector 2, wherein the nerve growth factor layer 6 becomes the middle layer of the nerve conduit because the nerve growth factor layer 6 is injected along the outer side wall of the cylindrical mold; the nerve stem cell layer 7 is injected along the lumen sidewall of the nerve growth factor layer 6 using the third syringe 3, and thus the nerve stem cell layer 7 becomes an inner layer of the nerve conduit.
The invention relates to a preparation method of an integrally molded magnetic induction nerve conduit, wherein when a pipeline is prepared by using a PDMS mold 4, two PDMS templates are adopted, a chemical etching method is used for manufacturing a strip-shaped groove on each PDMS template, then the two PDMS templates are subjected to oxygen beating in a plasma cleaning machine for 30 seconds to destroy the silicon-oxygen bond of the PDMS templates, then the two PDMS templates are tightly bonded under the condition that the two strip-shaped grooves correspond to each other to obtain the PDMS mold 4, and the two strip-shaped grooves form the pipeline.
The invention discloses a preparation method of an integrated forming magnetic induction nerve conduit, wherein when a cylindrical mold is placed in a pipeline, the cylindrical mold and the pipeline are coaxially arranged.
As shown in fig. 2, the nerve conduit is manufactured by a micro-fluidic control method, and comprises an outer layer, a middle layer and an inner layer, wherein the outer layer is a magnetic nanoparticle layer 5, the middle layer is a nerve growth factor layer 6, and the inner layer is a nerve stem cell layer 7, and the magnetic nanoparticles 501, the nerve growth factors and the nerve stem cells in the nerve conduit can promote the repair of nerve defects, so that the effect is better.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.

Claims (6)

1. The preparation method of the integrally formed magnetic induction nerve conduit is characterized by comprising the following steps of:
a pipe is prepared by using the PDMS mold,
placing a cylindrical mold in the pipeline, and leaving a gap between the outer side wall of the cylindrical mold and the inner side wall of the pipeline,
respectively injecting a magnetic nanoparticle layer and a nerve growth factor layer into the gap by a microfluidic method, wherein the magnetic nanoparticle layer is injected along the inner side wall of the pipeline, the nerve growth factor layer is injected along the outer side wall of the cylindrical mold,
freeze-drying the magnetic nanoparticle layer and the nerve growth factor layer, extracting the cylindrical mold from the inner cavity of the nerve growth factor layer after freeze-drying,
injecting a neural stem cell layer along the inner cavity side wall of the nerve growth factor layer by using a microfluidic method,
and (4) removing the PDMS mold to obtain the nerve conduit.
2. The method for preparing an integrally formed magnetically inductive nerve conduit as claimed in claim 1, wherein: the magnetic nanoparticle layer comprises magnetic nanoparticles and a material wrapping the magnetic nanoparticles, wherein the material wrapping the magnetic nanoparticles is at least one of PLGA, chitosan and GelMA.
3. The method for preparing an integrally formed magnetically inductive nerve conduit as claimed in claim 2, wherein: the nerve growth factor layer is a solution containing nerve growth factors, BSA (bovine serum albumin) and a water-soluble material, the nerve growth factors are at least one of HIF-1 alpha and rapamycin, and the water-soluble material is at least one of PVA (polyvinyl acetate), silk fibroin and type I collagen.
4. The method for preparing an integrally formed magnetically inductive nerve conduit as claimed in claim 3, wherein: the neural stem cell layer is hydrogel containing neural stem cells, and the hydrogel is at least one of PEG, gelatin and collagen.
5. The method for preparing an integrally formed magnetically inductive nerve conduit as claimed in claim 4, wherein: when the pipeline is prepared by using the PDMS mold, two PDMS templates are adopted, a strip-shaped groove is formed in each PDMS template by using a chemical etching method, then the two PDMS templates are subjected to oxygen beating in a plasma cleaning machine for 30 seconds to destroy a silicon-oxygen bond of the PDMS templates, the two PDMS templates are tightly bonded under the condition that the two strip-shaped grooves correspond to each other, the PDMS mold is obtained, and the pipeline is composed of the two strip-shaped grooves.
6. The method for preparing an integrally formed magnetically inductive nerve conduit as claimed in claim 5, wherein: when a cylindrical mold is placed in the pipeline, the cylindrical mold is arranged coaxially with the pipeline.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113577382A (en) * 2021-08-12 2021-11-02 东南大学 Peripheral nerve injury repair system
CN115382022A (en) * 2022-05-12 2022-11-25 大连工业大学 Continuous preparation method of nerve conduit material based on sodium alginate and krill protein

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081552A1 (en) * 2000-04-19 2001-11-01 Iowa State University Research Foundation, Inc. Patterned substrates and methods for nerve regeneration
US20030060836A1 (en) * 2000-12-05 2003-03-27 Shu Wang Polymer and nerve guide conduits formed thereof
CN1843307A (en) * 2005-04-07 2006-10-11 首都医科大学北京神经科学研究所 Double-layer artificial nerve catheter and preparation method thereof
CN101439205A (en) * 2008-12-29 2009-05-27 上海市第六人民医院 Nerve catheter as well as preparation method and application thereof
CN102048595A (en) * 2010-12-21 2011-05-11 东华大学 Degradable nerve conduit with highly-oriented tube-in-tube structure and manufacturing method thereof
CN102343114A (en) * 2011-10-24 2012-02-08 南通大学 Tissue engineering nerve graft and application thereof
CN106166306A (en) * 2016-08-24 2016-11-30 浙江省人民医院 A kind of bionical nerve trachea of composite dry cell and preparation method thereof
US20170296193A1 (en) * 2008-01-25 2017-10-19 The Johns Hopkins University Biodegradable nerve guides

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081552A1 (en) * 2000-04-19 2001-11-01 Iowa State University Research Foundation, Inc. Patterned substrates and methods for nerve regeneration
US20030060836A1 (en) * 2000-12-05 2003-03-27 Shu Wang Polymer and nerve guide conduits formed thereof
CN1843307A (en) * 2005-04-07 2006-10-11 首都医科大学北京神经科学研究所 Double-layer artificial nerve catheter and preparation method thereof
US20170296193A1 (en) * 2008-01-25 2017-10-19 The Johns Hopkins University Biodegradable nerve guides
CN101439205A (en) * 2008-12-29 2009-05-27 上海市第六人民医院 Nerve catheter as well as preparation method and application thereof
CN102048595A (en) * 2010-12-21 2011-05-11 东华大学 Degradable nerve conduit with highly-oriented tube-in-tube structure and manufacturing method thereof
CN102343114A (en) * 2011-10-24 2012-02-08 南通大学 Tissue engineering nerve graft and application thereof
CN106166306A (en) * 2016-08-24 2016-11-30 浙江省人民医院 A kind of bionical nerve trachea of composite dry cell and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
韩晓军: "《生物功能化界面》", 31 January 2017 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113577382A (en) * 2021-08-12 2021-11-02 东南大学 Peripheral nerve injury repair system
CN115382022A (en) * 2022-05-12 2022-11-25 大连工业大学 Continuous preparation method of nerve conduit material based on sodium alginate and krill protein
CN115382022B (en) * 2022-05-12 2023-08-22 大连工业大学 Continuous preparation method of nerve conduit material based on sodium alginate and krill protein

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