CN111743655A - Experiment rat cerebrospinal fluid extraction device - Google Patents
Experiment rat cerebrospinal fluid extraction device Download PDFInfo
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- CN111743655A CN111743655A CN202010596954.9A CN202010596954A CN111743655A CN 111743655 A CN111743655 A CN 111743655A CN 202010596954 A CN202010596954 A CN 202010596954A CN 111743655 A CN111743655 A CN 111743655A
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- cerebrospinal fluid
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- fluid extraction
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- 210000001175 cerebrospinal fluid Anatomy 0.000 title claims abstract description 69
- 238000000605 extraction Methods 0.000 title claims abstract description 44
- 238000002474 experimental method Methods 0.000 title description 9
- 241000700159 Rattus Species 0.000 claims description 30
- 241000700157 Rattus norvegicus Species 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 2
- 241000699666 Mus <mouse, genus> Species 0.000 claims 3
- 241000699670 Mus sp. Species 0.000 claims 1
- 238000000034 method Methods 0.000 description 8
- 238000001802 infusion Methods 0.000 description 7
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 210000001767 medulla oblongata Anatomy 0.000 description 3
- 208000034693 Laceration Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 210000001638 cerebellum Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000001951 dura mater Anatomy 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000007917 intracranial administration Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 238000010146 3D printing Methods 0.000 description 1
- 241001269524 Dura Species 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000036267 drug metabolism Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000000103 occipital bone Anatomy 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/80—Suction pumps
- A61M1/81—Piston pumps, e.g. syringes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2250/00—Specially adapted for animals
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Anesthesiology (AREA)
- Hematology (AREA)
- Surgery (AREA)
- Biomedical Technology (AREA)
- Vascular Medicine (AREA)
- Animal Husbandry (AREA)
- External Artificial Organs (AREA)
Abstract
The invention discloses an experimental rat cerebrospinal fluid extraction device, relates to the field of experimental instruments, and is used for optimizing the structure of the experimental rat cerebrospinal fluid extraction device. The experimental rat cerebrospinal fluid extraction device comprises a support piece, a positioning piece and an extraction pipeline. The support member has a support surface; the positioning piece is arranged on the support piece and is provided with a positioning inclined plane for supporting the body of the laboratory mouse; the included angle between the positioning inclined plane and the supporting surface is a set value; the extraction line is configured to extract cerebrospinal fluid of a laboratory mouse positioned on the positioning ramp. The technical scheme has reasonable structure and convenient use.
Description
Technical Field
The invention relates to the field of experimental instruments, in particular to an experimental rat cerebrospinal fluid extraction device.
Background
The method for collecting the cerebrospinal fluid of the rat comprises a method for drawing the cerebrospinal fluid by cutting the dura mater under direct vision and a method for drawing the cerebrospinal fluid by puncturing the medulla oblongata pool through the skin. Among them, the direct-view dura mater laceration cerebrospinal fluid extraction method requires anesthetizing a rat, fixing and preparing skin, incising skin and muscle tissue, and then obtaining cerebrospinal fluid by using a collecting device. The percutaneous puncture cerebellum medulla oblongata extraction cerebrospinal fluid method needs a professional small animal brain locator to determine the needle inserting position and depth.
The inventor finds that at least the following problems exist in the prior art:
(1) the direct-view dura mater-laceration cerebrospinal fluid extraction method is complicated to operate, the skin needs to be sutured after the operation if the rat needs to survive continuously, the operation is carried out in a sterile environment, and the requirements on technology, equipment and environment are relatively high.
(2) The percutaneous puncture cerebellum medulla oblongata extraction cerebrospinal fluid method needs a professional small animal brain positioning instrument to determine the needle inserting position and depth, so the cost is high and the operation is inconvenient; if the positioning is carried out by visual inspection, the depth of the inserted needle is difficult to judge, the technical difficulty is high, and the cerebrospinal fluid is difficult to take out when the operation is improper or the bloody cerebrospinal fluid is obtained due to the damage to other tissues.
In conclusion, the technical difficulty of collecting rat cerebrospinal fluid is relatively high, a professional positioning instrument is usually required for assisting operation, the requirement on the skill of an operator is high, and a large amount of manpower and material resources are consumed.
Disclosure of Invention
The invention provides a cerebrospinal fluid extraction device for an experimental rat, which is used for conveniently extracting cerebrospinal fluid of the experimental rat.
The embodiment of the invention provides an experimental rat cerebrospinal fluid extraction device, which comprises:
a support having a support surface;
the positioning piece is arranged on the supporting surface and is provided with a positioning inclined surface for supporting the body of the laboratory mouse; the included angle between the positioning inclined plane and the supporting surface is a set value; and
and a drawing line configured to draw cerebrospinal fluid of the laboratory mouse positioned on the positioning slope.
In some embodiments, the set value is 20-25 °.
In some embodiments, the positioning member further has an arc surface for supporting the neck of the laboratory mouse, and the arc surface intersects with the positioning slope.
In some embodiments, the experimental rat cerebrospinal fluid extraction device further comprises:
and the head fixing piece is provided with an inner concave part for clamping the head of the laboratory mouse and can be rotatably arranged on the supporting piece.
In some embodiments, the head fixing member is located on a side of the positioning member where the top end of the positioning slope is located.
In some embodiments, the experimental rat cerebrospinal fluid extraction device further comprises:
the mounting seat is fixed on the supporting piece, and the head fixing piece is rotatably mounted on the mounting seat and can be adjusted in rotating angle.
In some embodiments, the extraction line comprises:
an injector;
the needle assembly comprises a pipeline and a needle communicated with one end of the pipeline; and
a switching valve disposed between the injector and the other end of the pipe; the switching valve is configured to control the syringe and the conduit to switch between a communicating state and a non-communicating state by switching a valve position.
In some embodiments, the experimental rat cerebrospinal fluid extraction device further comprises:
a fixing seat, the switching valve being installed to the fixing seat.
In some embodiments, the fixing seat is located on one side of the positioning element.
The experiment mouse cerebrospinal fluid draw-out device that above-mentioned technical scheme provided has the location inclined plane that supports the experiment mouse, and the cerebrospinal fluid extraction in-process, the health of experiment mouse is no longer flat, but laminating location inclined plane, and the head of experiment mouse is located outside the inclined plane, has made things convenient for cerebrospinal fluid extraction experiment operation. The structural design enables the sampling part of the laboratory mouse to be well exposed, so that the technical difficulty of collecting cerebrospinal fluid is effectively reduced, the cerebrospinal fluid extraction operation is quick, simple and convenient, the accuracy is high, the obtained cerebrospinal fluid is pure, and the blood pollution is less. The technical scheme is widely applicable to the research of related fields of drug metabolism and pharmacology.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
fig. 1 is a schematic structural diagram of an experimental rat cerebrospinal fluid extraction device provided in an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a cerebrospinal fluid extraction device for a laboratory rat provided by an embodiment of the present invention, which does not include a needle assembly and a syringe.
Detailed Description
The technical solution provided by the present invention is explained in more detail with reference to fig. 1 to 2.
Referring to fig. 1 and 2, an embodiment of the present invention provides a cerebrospinal fluid extraction device for an experimental rat, including a support 1, a positioning member 2, and an extraction pipeline 3. The support 1 has a support surface 11. The positioning piece 2 is provided with a positioning inclined plane 21 for supporting the body of the laboratory mouse, the head of the laboratory mouse is positioned outside the positioning inclined plane 21, and the positioning inclined plane 21 does not support the head of the laboratory mouse. The positioning slope 21 is partially or entirely flat. The included angle between the positioning inclined plane 21 and the supporting surface 11 is a set value. The aspiration line 3 is configured to aspirate cerebrospinal fluid of a laboratory mouse positioned on the positioning slope 21.
In some embodiments, the support 1 may be embodied as a flat plate, and other components are directly or indirectly mounted to the support 1. The support 1 is used to provide support and the support 1 has a support surface 11. The support 1 may be fabricated using three-dimensional printing. The support surface 11 is a horizontal plane, and the support surface 11 serves as a mounting base for the positioning element 2 and the extraction line 3.
The positioning member 2 is mounted on the support member 1, for example, by bolting, gluing, or the positioning member 2 and the support member 1 are three-dimensionally printed as a single body. The positioning piece 2 is provided with a positioning inclined plane 21 for supporting the body of the experimental mouse, and the included angle between the positioning inclined plane 21 and the supporting plane 11 is a set value.
Through location inclined plane 21 for laboratory mouse's health keeps specific gesture, fully exposes the position of inserting the needle, makes things convenient for operating personnel to confirm simultaneously and inserts the needle angle, so that carry out subsequent experimental operation. In some embodiments, the set value is 20 ° -25 °, specifically such as 20 °, 21 °, 22 °, 23 °, 24 °, 25 °, and so forth.
Referring to fig. 1, in some embodiments, the positioning member 2 further has an arc surface 22 for supporting a neck of a laboratory mouse, and the arc surface 22 intersects with the positioning inclined surface 21, and may directly intersect with the positioning inclined surface or may indirectly intersect with the positioning inclined surface through a transition surface. The cambered surface 22 can support the lower part of the neck of the laboratory mouse, so that the posture of the laboratory mouse is more stable.
Referring to fig. 1, the aspiration line 3 is configured to aspirate cerebrospinal fluid of a laboratory mouse positioned on the positioning ramp 21. In some embodiments, the aspiration line 3 includes a syringe 31, a needle assembly 32, and a switching valve 33. Needle assembly 32 includes a tube 321 and a needle 322 in communication with one end of tube 321. The switching valve 33 is provided between the syringe 31 and the other end of the pipe 321; the switching valve 33 is configured to control the syringe 31 and the piping 321 to switch between a communicating state and a non-communicating state by switching the valve position. The switching valve 33 may specifically be a medical three-way valve.
The use method of the experimental rat cerebrospinal fluid extraction device comprises the following steps: an operator performs positioning needle insertion by visual observation, changes the connection state of each pipeline 321 when collecting cerebrospinal fluid by using the disposable switching valve 33, rotates the switching valve 33 by another experimenter when the cerebrospinal fluid naturally flows out under the intracranial pressure, and switches to the injector 31 to slowly suck the cerebrospinal fluid. Whether the needle insertion position is reasonable and the depth is proper or not is accurately judged through the natural outflow of the cerebrospinal fluid. After the extraction is finished, the switching valve 33 can be rotated to release the pressure in the pipeline 321 and close the pipeline 321, so that the loss of cerebrospinal fluid or the generation of bloody cerebrospinal fluid due to the pressure change during withdrawal during needle withdrawal is prevented, the yield of clarified cerebrospinal fluid is improved, the experimental efficiency and the success rate are improved, and the technical difficulty is reduced.
Therefore, according to the technical scheme, the switching pipeline 321 is communicated, the principle that a certain pressure exists in the encephalic of the laboratory mouse is utilized, the cerebrospinal fluid naturally flows out to form an accurate needle inserting mark, and the technical requirements on operators are greatly reduced. If the intracranial pressure is reduced due to the use of drugs or other reasons and the cerebrospinal fluid cannot naturally flow out, the switching valve 33 can be adjusted to the state that the infusion needle is communicated with the syringe 31, and the syringe 31 is slightly pumped to enable the pipeline 321 to have a certain negative pressure. After the cerebrospinal fluid is taken out, the switching valve 33 is used for closing the pipeline 321, so that the backflow or pollution of the cerebrospinal fluid can be effectively avoided when the needle is withdrawn. In addition, the operation is simple, skin and muscle tissues do not need to be cut, the experimental environment requirement is low, and the survival rate of the rat is high after taking out the cerebrospinal fluid.
In some embodiments, the experimental rat cerebrospinal fluid extraction device further includes a fixing seat 6, and the switching valve 33 is installed on the fixing seat 6. Through fixing base 6 for extraction pipeline 3 integral erection is in support piece 1, and whole experiment rat cerebrospinal fluid draw-out device structure is compacter, and the experiment is more convenient, and the part is difficult for losing, the single accurate operation of being convenient for.
In some embodiments, the fixing seat 6 is located on one side of the positioning member 2, i.e. on one side in the length direction of the laboratory mouse. The injector 31 and the disposable switching valve 33 are both fixed on one side of the rat, the positions of all parts are reasonably arranged, and the extraction operation of the cerebrospinal fluid of the experimental rat can be carried out by one person and one hand.
Referring to fig. 1 and 2, in order to better determine the position of the laboratory mouse, in some embodiments, the laboratory mouse cerebrospinal fluid extraction device further comprises a head fixture 4, and the head fixture 4 has an inner recess 41 for catching the head of the laboratory mouse. The concave portion 41 is configured to be concave upward and curved, and the shape of the concave portion 41 matches the shape of the head of the mouse to more firmly grip the head of the mouse. The head mount 4 is rotatably mounted to the support 1.
Referring to fig. 1 and 2, in some embodiments, the experimental rat cerebrospinal fluid extraction device further includes a mounting seat 5, the mounting seat 5 is fixed to the support 1, and the head fixing member 4 is rotatably mounted to the mounting seat 5 and is adjustable in rotation angle. By rotating the angle of the head mount 4, the head mount 4 is switched between the chucking position and the non-chucking position. When the head fixing member 4 is located at the holding position, the body of the laboratory mouse is supported by the positioning inclined plane 21, and the head of the laboratory mouse is held by the head fixing member 4, so that the head of the laboratory mouse is stably fixed on the supporting member 1.
In some embodiments, the positioning ramp 21 has a top end and a bottom end, the top end being located higher than the bottom end. The head fixing member 4 is located on the side of the positioning member 2 where the tip of the positioning slope 21 is located.
Specific examples are described below to illustrate in detail the method of using the above described experimental rat cerebrospinal fluid extraction device.
The needle 322, the three-way disposable switching valve 33, and the 1mL syringe 31 are connected in sequence by a tube 321, and the switching valve 33 is adjusted to a state where the infusion needle is communicated with the atmosphere. Adult rats were anesthetized with an aqueous solution of pentobarbital sodium, and the neck was skinned and sterilized. The head of the rat is placed downwards and on the positioning inclined plane 21, the knob 7 is rotated to enable the head fixing piece 4 to be rotated in place, and the head of the laboratory rat is fixed by using the head fixing piece 4. The midpoint of the line connecting the lower parts of the ears of the rat is visually observed, and the central position of the depression (large hole of occipital bone) between the occipital protuberance and the first cervical vertebra is determined by touch as a needle insertion point. The needle head 322 and the head of the hand-held disposable intravenous infusion needle are vertical to the support 1 and slowly enter the needle at the needle inlet point. Carefully watching the infusion needle line 321, gradually adjusting the depth of the needle insertion, and stopping the needle insertion when clear cerebrospinal fluid is observed to flow out. The switching valve 33 is adjusted to the state of communicating the infusion needle with the syringe 31, and the cerebrospinal fluid is slowly extracted, preferably in a volume of 100 μ L or less. After the extraction is finished, the switching valve 33 is adjusted until the infusion needle is closed, the infusion needle is slowly extracted, the extracted cerebrospinal fluid is discharged into a plastic centrifuge tube, and the cerebrospinal fluid is frozen at minus 80 ℃ for storage.
In the description of the present invention, it is to be understood that the terms "central", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be considered as limiting the scope of the present invention.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: it is to be understood that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof, but such modifications or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (9)
1. The utility model provides a laboratory rat cerebrospinal fluid draw-off device which characterized in that includes:
a support (1) having a support surface (11);
a positioning member (2) which is mounted on the support surface (11) and has a positioning inclined surface (21) for supporting the body of the laboratory mouse; the included angle between the positioning inclined plane (21) and the supporting surface (11) is a set value; and
a suction line (3) configured to suck cerebrospinal fluid of a laboratory mouse placed on the positioning slope (21).
2. The experimental rat cerebrospinal fluid extraction device according to claim 1, wherein the set value is 20 ° to 25 °.
3. The laboratory rat cerebrospinal fluid extraction device according to claim 1, characterized in that the positioning member (2) further has an arc surface (22) for supporting the neck of the laboratory rat, and the arc surface (22) intersects with the positioning slope (21).
4. The experimental rat cerebrospinal fluid extraction device according to claim 1, further comprising:
a head fixing member (4) having an inner recess (41) for catching the head of the laboratory mouse, the head fixing member (4) being rotatably installed to the supporter (1).
5. The cerebrospinal fluid extraction device for the laboratory mice according to claim 4, characterized in that the head fixation member (4) is located on the side of the positioning member (2) where the top end of the positioning slope (21) is located.
6. The experimental rat cerebrospinal fluid extraction device according to claim 4, further comprising:
the mounting seat (5) is fixed on the supporting piece (1), and the head fixing piece (4) is rotatably mounted on the mounting seat (5) and can be adjusted in rotation angle.
7. The laboratory rat cerebrospinal fluid extraction device according to claim 1, characterized in that the extraction line (3) comprises:
an injector (31);
a needle assembly (32) comprising a conduit (321) and a needle (322) in communication with one end of the conduit (321); and
a switching valve (33) provided between the syringe (31) and the other end of the pipe (321); the switching valve (33) is configured to control the syringe (31) and the piping (321) to switch between a communicating state and a non-communicating state by switching a valve position.
8. The experimental rat cerebrospinal fluid extraction device according to claim 7, further comprising:
a fixed seat (6), the switching valve (33) being mounted to the fixed seat (6).
9. The experimental rat cerebrospinal fluid extraction device according to claim 7, characterized in that the fixing seat (6) is located at one side of the positioning member (2).
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CN202010596954.9A CN111743655A (en) | 2020-06-28 | 2020-06-28 | Experiment rat cerebrospinal fluid extraction device |
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CN202010596954.9A CN111743655A (en) | 2020-06-28 | 2020-06-28 | Experiment rat cerebrospinal fluid extraction device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022196602A1 (en) * | 2021-03-16 | 2022-09-22 | 国立大学法人東京大学 | Small animal securing device |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN201353141Y (en) * | 2009-02-10 | 2009-12-02 | 李卫 | Device for measuring intracranial pressure and sampling cerebrospinal fluid |
CN104665951A (en) * | 2013-11-29 | 2015-06-03 | 苏州药明康德新药开发有限公司 | Fixing device for assisting in collecting cerebrospinal fluid of rat and use method thereof |
CN107280802A (en) * | 2017-07-27 | 2017-10-24 | 王太林 | The sterile of one boar takes blood fixing device and fixed blood extracting method |
CN207785311U (en) * | 2017-05-17 | 2018-08-31 | 广州中医药大学 | A kind of operation console acquiring experiment cerebrospinal fluid of rats through oblongata pond |
-
2020
- 2020-06-28 CN CN202010596954.9A patent/CN111743655A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN201353141Y (en) * | 2009-02-10 | 2009-12-02 | 李卫 | Device for measuring intracranial pressure and sampling cerebrospinal fluid |
CN104665951A (en) * | 2013-11-29 | 2015-06-03 | 苏州药明康德新药开发有限公司 | Fixing device for assisting in collecting cerebrospinal fluid of rat and use method thereof |
CN207785311U (en) * | 2017-05-17 | 2018-08-31 | 广州中医药大学 | A kind of operation console acquiring experiment cerebrospinal fluid of rats through oblongata pond |
CN107280802A (en) * | 2017-07-27 | 2017-10-24 | 王太林 | The sterile of one boar takes blood fixing device and fixed blood extracting method |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022196602A1 (en) * | 2021-03-16 | 2022-09-22 | 国立大学法人東京大学 | Small animal securing device |
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Application publication date: 20201009 |