CN111743038A - Low-protein daily feed containing alanine and preparation method and application thereof - Google Patents

Low-protein daily feed containing alanine and preparation method and application thereof Download PDF

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CN111743038A
CN111743038A CN202010528500.8A CN202010528500A CN111743038A CN 111743038 A CN111743038 A CN 111743038A CN 202010528500 A CN202010528500 A CN 202010528500A CN 111743038 A CN111743038 A CN 111743038A
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protein
feed
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low protein
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朴香淑
龙沈飞
张冬竹
刘树蓬
邢庆
刘磊
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Hefei Huaheng Biological Engineering Co ltd
Anhui Huaheng Biotechnology Co Ltd
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Anhui Huaheng Biotechnology Co Ltd
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Abstract

The invention relates to a low-protein daily ration feed containing alanine, which consists of 97-99.9% of low-protein basic daily ration and 0.1-3% of alanine, wherein the content of crude protein in the low-protein basic daily ration is 10-25%. The feed effectively saves increasingly short protein resources, obviously reduces feed cost and breeding cost, obviously reduces nitrogen emission of animal wastes, and is beneficial to treating environmental pollution.

Description

Low-protein daily feed containing alanine and preparation method and application thereof
Technical Field
The invention relates to a low-protein daily feed containing alanine, a preparation method and application thereof.
Background
As a big breeding country in China, animal protein feed resources are imported for a long time and become bottlenecks restricting the development of feed industry and breeding industry in China. In addition, the nitrogen emission generated by animal excrement caused by animal feeding protein feed is a great problem in environmental pollution treatment. The low-protein basic ration for animals is added with proper types and quantities of industrial amino acids and reduces the protein level in the basic ration so as to reduce nitrogen emission and be beneficial to the treatment of environmental pollution. How to scientifically screen and add industrial amino acid with proper types and quantities to promote animal growth and the like becomes a technical problem to be solved urgently.
Disclosure of Invention
The invention aims to provide a low-protein daily ration feed containing alanine, which consists of 97-99.9% of low-protein basic daily ration and 0.1-3% of alanine, wherein the crude protein content of the low-protein basic daily ration is 10-25%.
In a preferred technical scheme of the invention, the alanine content in the low-protein daily feed is 0.2-2%, preferably 0.4-1.5%, more preferably 0.6-1.2%, and still more preferably 0.8-1.0%.
In a preferred embodiment of the present invention, the alanine is any one or a combination of L-alanine, DL-alanine, D-alanine, and β -alanine.
In the preferable technical scheme of the invention, the content of crude protein in the low-protein basic ration is 13-22%, preferably 15-20%, and more preferably 16-19%.
In a preferred technical scheme of the invention, the content of crude protein in the low-protein basic ration is reduced by 2-4% compared with the content of crude protein in the animal normal protein basic ration specified by animal feed standards.
In a preferred technical scheme of the invention, the normal basal ration for animals is any one of a normal basal ration for chickens, a normal basal ration for ducks, a normal basal ration for geese, a normal basal ration for pigs, a normal basal ration for cows, a normal basal ration for horses, a normal basal ration for donkeys, a normal basal ration for cats, a normal basal ration for dogs, a normal basal ration for fishes, a normal basal ration for rabbits and a normal basal ration for sheep.
In a preferred embodiment of the present invention, the crude protein content of the normal basal ration for animals is selected from a specified amount according to any one of the criteria in tables 1 to 5.
TABLE 1 national Standard of Compound feed for livestock and poultry (CNS3027-2000)
Figure BDA0002534384740000021
Figure BDA0002534384740000031
Figure BDA0002534384740000041
Table 2 "T/CFIAS 002-2018 laying hen and broiler chicken compound feed
Figure BDA0002534384740000042
Table 3 "T/CFIAS 002-2018 laying hen and broiler chicken compound feed
Figure BDA0002534384740000043
Table 4' T/CFIAS002-2018 laying hen and broiler chicken compound feed
Figure BDA0002534384740000044
Table 5 Compound feed for T/CFIAS001-2018 piglets and growing-finishing pigs
Figure BDA0002534384740000045
In the preferred technical scheme of the invention, the components and the mass percent of the components forming the low-protein basic ration are as follows: 60-75% of corn, 15-30% of soybean meal, 0.5-5% of corn protein powder, 1-5% of fish meal, 0-4% of soybean oil, 0-2% of monocalcium phosphate, 0-4% of stone powder, 0-1% of salt, 0-1% of L-lysine, 0-1% of methionine, 0-1% of threonine, 0-1% of tryptophan, 0-1% of chromium oxide and 0-2% of basic ration premix.
In the preferred technical scheme of the invention, the components and the mass percent of the components forming the low-protein basic ration are as follows: 70.24 percent of corn, 20.00 percent of soybean meal, 1.58 percent of corn protein powder, 2.60 percent of fish meal, 1.10 percent of soybean oil, 1.50 percent of monocalcium phosphate, 1.30 percent of stone powder, 0.30 percent of salt, 0.30 percent of L-lysine, 0.18 percent of methionine, 0.15 percent of threonine, 0.25 percent of chromium oxide and 0.50 percent of basic ration premix.
In the preferred technical scheme of the invention, the components and the mass percent of the components forming the low-protein basic ration are as follows: 74.49% of corn, 16.00% of soybean meal, 0.96% of corn protein powder, 2.30% of fish meal, 2.00% of soybean oil, 1.50% of monocalcium phosphate, 1.10% of stone powder, 0.30% of salt, 0.32% of L-lysine, 0.09% of methionine, 0.17% of threonine, 0.02% of tryptophan, 0.25% of chromium oxide and 0.50% of basic ration premix.
In a preferred technical scheme of the invention, in the components of the low-protein basic ration, each kilogram of basic ration premix comprises 11,000IU of vitamin A, 3,025IU of vitamin D, 22mg of vitamin E, 2.2mg of vitamin K3, 1.65mg of thiamine, 6.6mg of riboflavin, 3.3mg of pyridoxine, 17.6 mu g of cobalamin, 22mg of nicotinic acid, 13.2mg of pantothenic acid, 0.33mg of folic acid, 88 mu g of biotin, 500mg of choline chloride, 48mg of iron, 96.6mg of zinc, 101.76mg of manganese, 10mg of copper, 0.05mg of selenium, 0.96mg of iodine and 0.3mg of cobalt.
In the preferred technical scheme of the invention, the components and mass percentages of the low-protein basic ration are as follows: 50-72% of corn, 10-20% of soybean meal, 5-14% of puffed full-fat soybean, 1-5% of fish meal, 1-5% of whey powder, 1-5% of soybean oil, 0-2% of calcium hydrophosphate, 0-2% of mountain flour, 0-1% of salt, 0-1% of L-lysine hydrochloride, 0-0.5% of DL-methionine, 0-0.5% of L-threonine, 0-0.5% of L-tryptophan, 0-0.5% of zinc oxide, 0-0.5% of chromium oxide and 0-1% of basic ration premix.
In the preferred technical scheme of the invention, the components and mass percentages of the low-protein basic ration are as follows: 61.89% of corn, 9.00% of soybean meal, 14.00% of puffed full-fat soybean, 5.00% of fish meal, 3.00% of whey powder, 3.07% of soybean oil, 1.00% of calcium hydrophosphate, 0.80% of stone powder, 0.30% of salt, 0.61% of L-lysine hydrochloride, 0.10% of DL-methionine, 0.22% of L-threonine, 0.06% of L-tryptophan, 0.20% of zinc oxide, 0.25% of chromium oxide and 0.50% of basic ration premix.
In the preferred technical scheme of the invention, the components and mass percentages of the low-protein basic ration are as follows: 70.71 percent of corn, 10.00 percent of soybean meal, 7.00 percent of puffed full-fat soybean, 2.00 percent of fish meal, 3.00 percent of whey powder, 3.15 percent of soybean oil, 0.88 percent of calcium hydrophosphate, 0.96 percent of stone powder, 0.30 percent of salt, 0.75 percent of L-lysine hydrochloride, 0.14 percent of DL-methionine, 0.28 percent of L-threonine, 0.08 percent of L-tryptophan, 0.25 percent of chromium oxide and 0.50 percent of basic ration premix.
In the preferable technical scheme of the invention, in the components of the low-protein basic ration, each kilogram of basic ration premix comprises the following components in percentage by weight: 12,000 IU; vitamin D3: 2,500 IU; vitamin E: 30 IU; vitamin K3: 30 mg; vitamin B12: 12 mu g of the solution; riboflavin: 4 mg; pantothenic acid: 15 mg; nicotinic acid: 40 mg; choline chloride: 400 mg; folic acid: 0.7 mg; vitamin B1: 1.5 mg; vitamin B6: 3 mg; biotin: 0.1 mg; manganese: 40 mg; iron; 90 mg; zinc: 100 mg; copper: 8.8 mg; iodine: 0.35mg and selenium: 0.3 mg.
The invention also aims to provide a preparation method of the low-protein daily feed containing alanine, wherein the low-protein daily feed consists of 97-99.9% of low-protein basic daily feed and 0.1-3% of alanine, the crude protein content of the low-protein basic daily feed is 10-25%, and the low-protein basic daily feed and the alanine are weighed according to required amounts and uniformly mixed to obtain the low-protein daily feed.
In a preferred technical scheme of the invention, the alanine content in the low-protein daily feed is 0.2-2%, preferably 0.4-1.5%, more preferably 0.6-1.2%, and still more preferably 0.8-1.0%.
In a preferred embodiment of the present invention, the alanine is any one or a combination of L-alanine, DL-alanine, D-alanine, and β -alanine.
In the preferable technical scheme of the invention, the content of crude protein in the low-protein basic ration is 13-22%, preferably 15-20%, and more preferably 16-19%.
In a preferred technical scheme of the invention, the content of crude protein in the low-protein basic ration is reduced by 2-4% compared with the content of crude protein in the animal normal protein basic ration specified by animal feed standards.
In a preferred technical scheme of the invention, the normal basal ration for animals is any one of a normal basal ration for chickens, a normal basal ration for ducks, a normal basal ration for geese, a normal basal ration for pigs, a normal basal ration for cows, a normal basal ration for horses, a normal basal ration for donkeys, a normal basal ration for cats, a normal basal ration for dogs, a normal basal ration for fishes, a normal basal ration for rabbits and a normal basal ration for sheep.
In a preferred embodiment of the present invention, the crude protein content of the normal basal ration for animals is selected from a specified amount according to any one of the criteria in tables 1 to 5.
In the preferred technical scheme of the invention, the components and the mass percent of the components forming the low-protein basic ration are as follows: 60-75% of corn, 15-30% of soybean meal, 0.5-5% of corn protein powder, 1-5% of fish meal, 0-4% of soybean oil, 0-2% of monocalcium phosphate, 0-4% of stone powder, 0-1% of salt, 0-1% of L-lysine, 0-1% of methionine, 0-1% of threonine, 0-1% of tryptophan, 0-1% of chromium oxide and 0-2% of basic ration premix.
In the preferred technical scheme of the invention, the components and the mass percent of the components forming the low-protein basic ration are as follows: 70.24 percent of corn, 20.00 percent of soybean meal, 1.58 percent of corn protein powder, 2.60 percent of fish meal, 1.10 percent of soybean oil, 1.50 percent of monocalcium phosphate, 1.30 percent of stone powder, 0.30 percent of salt, 0.30 percent of L-lysine, 0.18 percent of methionine, 0.15 percent of threonine, 0.25 percent of chromium oxide and 0.50 percent of basic ration premix.
In the preferred technical scheme of the invention, the components and the mass percent of the components forming the low-protein basic ration are as follows: 74.49% of corn, 16.00% of soybean meal, 0.96% of corn protein powder, 2.30% of fish meal, 2.00% of soybean oil, 1.50% of monocalcium phosphate, 1.10% of stone powder, 0.30% of salt, 0.32% of L-lysine, 0.09% of methionine, 0.17% of threonine, 0.02% of tryptophan, 0.25% of chromium oxide and 0.50% of basic ration premix.
In a preferred technical scheme of the invention, in the components of the low-protein basic ration, each kilogram of basic ration premix comprises 11,000IU of vitamin A, 3,025IU of vitamin D, 22mg of vitamin E, 2.2mg of vitamin K3, 1.65mg of thiamine, 6.6mg of riboflavin, 3.3mg of pyridoxine, 17.6 mu g of cobalamin, 22mg of nicotinic acid, 13.2mg of pantothenic acid, 0.33mg of folic acid, 88 mu g of biotin, 500mg of choline chloride, 48mg of iron, 96.6mg of zinc, 101.76mg of manganese, 10mg of copper, 0.05mg of selenium, 0.96mg of iodine and 0.3mg of cobalt.
In the preferred technical scheme of the invention, the components and mass percentages of the low-protein basic ration are as follows: 50-72% of corn, 10-20% of soybean meal, 5-14% of puffed full-fat soybean, 1-5% of fish meal, 1-5% of whey powder, 1-5% of soybean oil, 0-2% of calcium hydrophosphate, 0-2% of mountain flour, 0-1% of salt, 0-1% of L-lysine hydrochloride, 0-0.5% of DL-methionine, 0-0.5% of L-threonine, 0-0.5% of L-tryptophan, 0-0.5% of zinc oxide, 0-0.5% of chromium oxide and 0-1% of basic ration premix.
In the preferred technical scheme of the invention, the components and mass percentages of the low-protein basic ration are as follows: 61.89% of corn, 9.00% of soybean meal, 14.00% of puffed full-fat soybean, 5.00% of fish meal, 3.00% of whey powder, 3.07% of soybean oil, 1.00% of calcium hydrophosphate, 0.80% of stone powder, 0.30% of salt, 0.61% of L-lysine hydrochloride, 0.10% of DL-methionine, 0.22% of L-threonine, 0.06% of L-tryptophan, 0.20% of zinc oxide, 0.25% of chromium oxide and 0.50% of basic ration premix.
In the preferred technical scheme of the invention, the components and mass percentages of the low-protein basic ration are as follows: 70.71 percent of corn, 10.00 percent of soybean meal, 7.00 percent of puffed full-fat soybean, 2.00 percent of fish meal, 3.00 percent of whey powder, 3.15 percent of soybean oil, 0.88 percent of calcium hydrophosphate, 0.96 percent of stone powder, 0.30 percent of salt, 0.75 percent of L-lysine hydrochloride, 0.14 percent of DL-methionine, 0.28 percent of L-threonine, 0.08 percent of L-tryptophan, 0.25 percent of chromium oxide and 0.50 percent of basic ration premix.
In the preferable technical scheme of the invention, in the components of the low-protein basic ration, each kilogram of basic ration premix comprises the following components in percentage by weight: 12,000 IU; vitamin D3: 2,500 IU; vitamin E: 30 IU; vitamin K3: 30 mg; vitamin B12: 12 mu g of the solution; riboflavin: 4 mg; pantothenic acid: 15 mg; nicotinic acid: 40 mg; choline chloride: 400 mg; folic acid: 0.7 mg; vitamin B1: 1.5 mg; vitamin B6: 3 mg; biotin: 0.1 mg; manganese: 40 mg; iron; 90 mg; zinc: 100 mg; copper: 8.8 mg; iodine: 0.35mg and selenium: 0.3 mg.
Another object of the present invention is to provide the use of a low protein ration feed comprising alanine for the preparation of a low protein ration feed for animals.
In a preferred technical scheme of the invention, the animal low-protein daily feed is selected from any one of chicken low-protein daily feed, duck low-protein daily feed, goose low-protein daily feed, pig low-protein daily feed, fish low-protein daily feed, cattle low-protein daily feed, cat low-protein daily feed, dog low-protein daily feed, rabbit low-protein daily feed and sheep low-protein daily feed.
Unless otherwise indicated, when the present invention relates to percentages between liquids, said percentages are volume/volume percentages; the invention relates to the percentage between liquid and solid, said percentage being volume/weight percentage; the invention relates to the percentages between solid and liquid, said percentages being weight/volume percentages; the balance being weight/weight percent.
Compared with the prior art, the invention has the following beneficial technical effects:
1. the method scientifically screens the contents of the low-protein basic ration and the alanine in the low-protein daily ration feed, so that the increasingly deficient protein resources are effectively saved, the feed cost and the breeding cost are obviously reduced, the nitrogen emission in animal excrement is obviously reduced, and the environmental pollution treatment is facilitated; the daily gain, daily feed intake and feed conversion rate of animals are improved; and thirdly, the growth performance and the oxidation resistance of the animals are obviously improved, the influence of the environmental factors on the oxidative stress of the animal growth is reduced or even avoided, the immunity of the animals is obviously provided, and the edible mouthfeel of the animals is obviously improved.
2. The preparation method has the advantages of simple and convenient operation, obvious cost reduction, suitability for industrial production and the like.
Drawings
Figure 1 effect of low protein ration feed containing alanine according to the invention on average daily gain of weaned piglets. The full-period ADG is taken as a target point, and the target point is obtained by analyzing a single-slope broken line model under the condition of low-protein basic ration. If x is less than or equal to the required amount, y is 421.5-0.1567 (438.3-x); r2 ═ 0.87.
Detailed Description
The present invention is described below with reference to examples, but the scope of the present invention is not limited to the examples.
Example 1Preparation and application of low-protein daily ration feed containing alanine for chicken
The required amount of each component was weighed as shown in table 6 and mixed well to obtain normal protein basal ration (early stage), normal protein basal ration (late stage), low protein basal ration (early stage) and low protein basal ration (late stage). The nutrient levels of each basal diet were calculated (see table 7).
TABLE 6 basic diet composition
Figure BDA0002534384740000111
Figure BDA0002534384740000121
The composition of the premix for each kilogram of basic daily ration is as follows: vitamin a, 11,000 IU; vitamin D, 3,025 IU; vitamin E, 22 mg; vitamin K3, 2.2 mg; thiamine, 1.65 mg; riboflavin, 6.6 mg; pyridoxine, 3.3 mg; cobalamin, 17.6 micrograms; nicotinic acid, 22 mg; pantothenic acid, 13.2 mg; folic acid, 0.33 mg; biotin, 88 μ g; choline chloride, 500 mg; iron, 48 mg; zinc, 96.6 mg; manganese, 101.76 mg; copper, 10 mg; selenium, 0.05 mg; iodine, 0.96mg and cobalt 0.3 mg.
TABLE 7 basal diet nutritional levels
Figure BDA0002534384740000122
1.1 test period
The test period is totally 42 days and is divided into a test early period (d 1-21) and a test later period (d 22-42).
1.2 test grouping
294 healthy AA (Aipaiyijia) broilers are selected for the test, the initial weight is 45.04 +/-0.92 g, the broilers are completely randomly divided into 7 groups according to the weight, each group is 7 in number, and each group is 6 in number. The animals were raised in columns with each repeat unit, and the feed for each group is shown in Table 8.
TABLE 8 basal diet feed feeding regimen
Figure BDA0002534384740000131
1.3 Breeding management
The test was carried out in the nutrient metabolism room of the feed industry center of the department of agriculture of the university of agriculture in China. The method is characterized in that a totally enclosed henhouse is adopted, the temperature, the humidity, the ventilation strength, the carbon dioxide and ammonia concentration in the henhouse are automatically controlled, the temperature of the henhouse is gradually reduced to 24-26 ℃ from 37 ℃ in the test period, experimental broiler chicken is raised in cages and raised, and a stainless steel adjustable trough and a nipple type water fountain are adopted. The powder is used for feeding, and the food is taken and drunk freely. Periodically cleaning the excrement and continuously illuminating. Disinfecting chickens twice a week during the feeding period, and disinfecting the environment outside the henhouse once; the broiler chicken immunization program is carried out according to the conventional method.
1.4 detection index and determination method
1.4.1 growth Performance
At the beginning and end of the experiment, the individual weight of the broilers and the weight of the feed were weighed, and the Average Daily Gain (ADG), Average Daily Feed Intake (ADFI) and Feed Conversion Ratio (FCR) were calculated.
1.4.2 antioxidant and immune function
Randomly selecting 2 broilers with the weight close to the average value of the repetition at 21 days of age and 42 days of age respectively, collecting blood in a wing vein by 4mL, centrifuging at 3000r/min for 10min, and subpackaging supernate in 0.5mL Eppendorf tubes for preservation at minus 20 ℃. The supernatant was collected and tested for superoxide dismutase (SOD) using a test kit purchased from Nanjing, institute of bioengineering (China).
The test results are shown in tables 9-10.
The method scientifically screens the contents of the low-protein basic ration and the alanine in the low-protein daily ration feed, can improve the daily gain, daily feed intake and feed conversion rate of the broiler chickens, obviously improves the growth performance and oxidation resistance of the broiler chickens, reduces or even avoids the oxidative stress influence of environmental factors on the growth of the broiler chickens, and obviously improves the immunity of the broiler chickens.
TABLE 9 influence of alanine on growth Performance of broilers
Figure BDA0002534384740000151
Figure BDA0002534384740000161
Note: BW: body weight; ADG: average daily gain; ADFI: average daily food intake; FCR: the feed conversion rate; SEM: the mean value is standard error.
TABLE 10 influence of alanine on antioxidant and immune function of broiler sera
Figure BDA0002534384740000171
Note: SOD (superoxide dismutase): superoxide dismutase; SEM: mean standard error
Example 2Preparation and application of low-protein daily ration feed containing alanine for piglets
The required amount of each component was weighed as shown in table 11 and mixed well to obtain normal protein basal ration (early stage), normal protein basal ration (late stage), low protein basal ration (early stage) and low protein basal ration (late stage). The nutrient levels of each basal diet were calculated (see table 11).
TABLE 11 basic diet composition
Figure BDA0002534384740000181
The composition of the premix for each kilogram of basic daily ration is as follows: vitamin A: 12,000 IU; vitamin D3: 2,500 IU; vitamin E: 30 IU; vitamin K3: 30 mg; vitamin B12: 12 mu g of the solution; riboflavin: 4 mg; pantothenic acid: 15 mg; nicotinic acid: 40 mg; choline chloride: 400 mg; folic acid: 0.7 mg; vitamin B1: 1.5 mg; vitamin B6: 3 mg; biotin: 0.1 mg; manganese: 40 mg; iron; 90 mg; zinc: 100 mg; copper: 8.8 mg; iodine: 0.35 mg; selenium: 0.3 mg.
TABLE 12 basal diet nutritional levels
Figure BDA0002534384740000191
1.1 test period
The test period is 28 days in total, and the test period is divided into a front period (d 1-14) and a rear period (d 15-28).
1.2 test grouping
216 healthy Du multiplied by long multiplied by 32-day-old weaned pigs (average body weight 9.35 +/-2.42 kg) are selected, the weaned pigs are completely randomly divided into 6 groups according to the body weight and the sex, 6 pigs (each half of a male and a female) are repeated in each group, and the number of the pigs is 6.
TABLE 13 feeding protocols
Figure BDA0002534384740000192
Figure BDA0002534384740000201
1.3 Breeding management
The test is carried out in a conservation house of the Hebei Fengning test base of the Chinese agricultural university, a totally enclosed pigsty is adopted, the temperature, the humidity, the ventilation intensity, the carbon dioxide and the ammonia concentration in the pigsty are automatically controlled, the temperature of the pigsty is kept at 24-26 ℃ during the test period, and the test piglets are fed in columns and are fed at 1.5 × 1.5.5 m2In the ring, a plastic-sprayed floor with a leak seam, a stainless steel adjustable trough and a duckbill-type water fountain. The powder is fed, and the test period comprises free food intake and drinking.
1.4 detection index and determination method
1.4.1 growth Performance
In trials d0, d14, d28, piglet weight and Feed weight were weighed, respectively, and Average Daily Gain (ADG), Average Daily Feed Intake (ADFI) and weight Gain to Feed ratio (Gain: Feed, G: F) were calculated.
1.4.2 diarrhea Rate
Throughout the trial period, piglet diarrhea was recorded every morning and the diarrhea rate was calculated.
The results are shown in Table 14 and FIG. 1. The alanine is added into the low-protein basic ration of the weaned pig, so that the growth performance of the piglet can be obviously improved, and the diarrhea rate of the piglet is effectively reduced.
The above description of the specific embodiments of the present invention is not intended to limit the present invention, and those skilled in the art may make various changes and modifications according to the present invention without departing from the spirit of the present invention, which is defined in the appended claims.
TABLE 14 influence of alanine addition to basal ration on growth performance and diarrhea rate of weaned piglets
Figure BDA0002534384740000211
Figure BDA0002534384740000221
Note: ADG: average daily gain; ADFI: average feed intake; G/F: the material weight ratio.

Claims (10)

1. The low-protein daily ration feed containing alanine is characterized by consisting of 97-99.9% of low-protein basic daily ration and 0.1-3% of alanine, wherein the crude protein content of the low-protein basic daily ration is 10-25%.
2. The low protein ration feed of claim 1, wherein the crude protein content in the low protein base ration is reduced by 2-4% from the crude protein content in an animal normal protein base ration specified by animal feed standards.
3. The low protein ration feed according to any of the claims 1-2, wherein the amount of alanine in the low protein ration feed is between 0.2% and 2%, preferably between 0.4% and 1.5%, more preferably between 0.6% and 1.2%, still more preferably between 0.8% and 1.0%.
4. The low protein ration feed of any one of claims 1-3, wherein the alanine is selected from any one of L-alanine, DL-alanine, D-alanine, β -alanine, or a combination thereof.
5. The low protein ration feed according to any of the claims 1-4, wherein the crude protein content in the low protein base ration is 13-22%, preferably 15-20%, more preferably 16-19%.
6. The low protein ration feed of any one of claims 1 to 5, wherein the animal normal protein basal ration is selected from any one of a chicken basal ration, a duck basal ration, a goose basal ration, a pig basal ration, a cow basal ration, a horse basal ration, a donkey basal ration, a cat basal ration, a dog basal ration, a fish basal ration, a rabbit basal ration, and a sheep basal ration.
7. The low protein ration feed according to any one of claims 1 to 6, wherein the components and mass percentages thereof that make up the low protein basal ration are: 60-75% of corn, 15-30% of soybean meal, 0.5-5% of corn protein powder, 1-5% of fish meal, 0-4% of soybean oil, 0-2% of monocalcium phosphate, 0-4% of stone powder, 0-1% of salt, 0-1% of L-lysine, 0-1% of methionine, 0-1% of threonine, 0-1% of tryptophan, 0-1% of chromium oxide and 0-2% of basic ration premix.
8. The low protein ration feed according to any one of claims 1 to 6, wherein the components and mass percentages constituting the low protein basal ration are: 50-72% of corn, 10-20% of soybean meal, 5-14% of puffed full-fat soybean, 1-5% of fish meal, 1-5% of whey powder, 1-5% of soybean oil, 0-2% of calcium hydrophosphate, 0-2% of mountain flour, 0-1% of salt, 0-1% of L-lysine hydrochloride, 0-0.5% of DL-methionine, 0-0.5% of L-threonine, 0-0.5% of L-tryptophan, 0-0.5% of zinc oxide, 0-0.5% of chromium oxide and 0-1% of basic ration premix.
9. A method of preparing the low protein ration feed of any one of claims 1-8, consisting of 97% -99.9% of a low protein base ration having a crude protein content of 10% -25% and 0.1% -3% of alanine, by weighing the required amounts of the low protein base ration and alanine and mixing them homogeneously.
10. Use of the low protein ration feed according to any of the claims 1-8 or the low protein ration feed prepared by the method according to claim 9 for the preparation of a low protein ration feed for animals, preferably the low protein ration feed for animals is selected from any of the group consisting of chicken low protein ration feed, duck low protein ration feed, goose low protein ration feed, pig low protein ration feed, fish low protein ration feed, cow low protein ration feed, cat low protein ration feed, dog low protein ration feed, rabbit low protein ration feed, sheep low protein ration feed.
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