CN111733253A

CN111733253A - Marker for immune-related adverse reaction and application thereof

Info

Publication number: CN111733253A
Application number: CN202010846330.8A
Authority: CN
Inventors: 薛新颖; 景莹; 庄光磊; 刘锦; 臧学磊; 高杰
Original assignee: Beijing Xinnuo Weikang Technology Co ltd
Current assignee: Beijing Xinnuo Weikang Technology Co ltd
Priority date: 2020-08-21
Filing date: 2020-08-21
Publication date: 2020-10-02
Anticipated expiration: 2040-08-21
Also published as: WO2022037474A1; CN112557668B; CN111733253B; CN112557668A

Abstract

The present invention relates to immune-related adverse reaction biomarkers and uses thereof, comprising LCP1 and/or ADPGK. By adopting the Immune-related adverse reactions (IRAEs) biomarker and the application thereof, the invention provides the kit and the method which are helpful for early prediction and active intervention of Immune-related adverse reactions caused by anti-PD-1/PD-L1 antibody treatment, thereby improving the prognosis effect of patients.

Description

Marker for immune-related adverse reaction and application thereof

Technical Field

The invention belongs to the technical field of biological medicines, and particularly relates to a marker for immune-related adverse reactions and application thereof.

Background

Anti-programmed death receptor 1 (PD-1)/anti-programmed death ligand 1 (PD-L1) antibody therapy activates the body's Immune response and breaks Immune homeostasis, and Immune-related adverse reactions (iraEs) triggered thereby affect the organs of the body and, in severe cases, can lead to death. Pneumonia is the most common fatal injury of irAEs, leading to death in 10% of patients, accounting for 35% of the mortality associated with anti-PD-1/PD-L1 antibody treatment. Myocarditis is the most important lethal injury of irAEs with mortality rates of up to 50%. Thus, the discovery of irAEs biomarkers is determinative of the prediction of the ratio of therapeutic benefit to risk for patients receiving anti-PD-1/PD-L1 antibody treatment. T Cell Receptor (TCR) diversity, CD8+ T cell clonal expansion, tumor burden mutation (TMB) have been reported to have predictive irAEs potential, but these conclusions are either established in a one-way analysis or limited to a limited number of cases. Therefore, comprehensive analytical studies on the prediction of irAEs biomarkers are needed.

Disclosure of Invention

Based on the above-mentioned state of the art, the inventors tried to provide an Immune-related adverse effect (irAEs) biomarker, thereby providing a kit for early prediction and active intervention of Immune-related adverse effects caused by anti-PD-1/PD-L1 antibody therapy, and applications thereof. In order to realize the purpose of the invention, the following technical scheme is adopted:

one aspect of the invention relates to immune-related adverse reaction biomarkers comprising LCP1 and/or ADPGK. LCP1 belongs to actin-plastin family, and under the co-stimulation of CD3/CD2 or CD3/CD8, LCP promotes the activation of T cells by accelerating the transport of CD69 and CD25 to the surfaces of the T cells; ADPGK mediates metabolic switching in T cell activation, accelerates glycolysis, reduces mitochondrial respiration, and enhances T cell glucose uptake. The invention unexpectedly finds that LCP1 and ADPGK are biomarkers for predicting the occurrence of iraE, and particularly the AUC value of the combination of LCP1 and ADPGK reaches over 0.8.

Another aspect of the invention relates to a pre-test kit for immune-related adverse reactions comprising reagents for detecting the expression level of LCP1 and/or ADPGK. Preferably, the agent is an immunochemical agent.

The invention also relates to application of the marker or the pre-test kit in preparing a kit for predicting immune-related adverse reactions.

In a preferred embodiment of the invention, the immune-related side effects are immune-related side effects caused by anti-PD-1/PD-L1 antibody treatment. Such immune-related adverse reactions include, but are not limited to, pneumonia, myocarditis, colitis, pancreatitis, hypothyroidism, hyperthyroidism, thyroiditis, hypophysitis, type i diabetes, adrenal insufficiency, sarcoidosis, vitiligo, severe adverse skin reactions, thrombocytopenia, hepatitis, gastrointestinal toxicity, nervous system disorders, nephritis, uveitis. Particularly preferred immune-related adverse reactions include enzootic pneumonia.

In one aspect of the invention, the anti-PD-1 antibody includes, but is not limited to, nivolumab, pembrolizumab, cimicimab, and the anti-PD-L1 includes, but is not limited to, atelizumab, avizumab, and dulvacizumab.

In a preferred embodiment of the invention, the subject treated with the anti-PD-1/PD-L1 antibody includes, but is not limited to, lung adenocarcinoma; SKCM, cutaneous melanoma; PRAD, prostate cancer; BLCA, bladder urothelial cancer; MESO, mesothelioma; BRCA, breast invasive carcinoma; CESC, cervical squamous carcinoma and cervical adenocarcinoma; PAAD, pancreatic cancer; OV, ovarian serous cystadenocarcinoma; HNSC, head and neck squamous cell carcinoma; STAD, gastric adenocarcinoma; THCA, thyroid cancer; CHOL, cholangiocarcinoma; ACC, adrenocortical carcinoma; READ, rectal adenocarcinoma; COAD, colon cancer; LIHC, hepatocellular carcinoma; LGG, brain low-grade glioma; GBM, glioblastoma multiforme; UVM, uveal melanoma; UCS, uterine carcinosarcoma.

Advantageous effects

By adopting the Immune-related adverse reactions (IRAEs) biomarker and the application thereof, the invention provides the kit and the method which are helpful for early prediction and active intervention of Immune-related adverse reactions caused by anti-PD-1/PD-L1 antibody treatment, thereby improving the prognosis effect of patients.

Drawings

FIG. 1 is a graph showing calculation of iraE Report Odds Ratio (ROR) for 26 cancer types, and scoringA graph of the correlation between factors associated with immunotherapy response and ROR was estimated. a. Histological location of the different carcinogenesis (left) and the respective irAE ROR (right); b. spearman correlation test was performed for irAE ROR and 36 immunotherapy response-related factors, with the red bar representing positive correlation and the blue bar representing negative correlation. Indicates significant correlation (FDR)<0.05), irAE/immunotherapy consensus correlation factor expressed in orange, c. creation of bivariate model ir ae prediction effect evaluation of the combination of top 6 irAE significance correlation factors, Spearman R (Rs) calculation prediction and observed correlation between irAE ROR, square shades of color indicate Rs, size indicates significance of log-likelihood ratio test d.tcr diversity and combined prediction effect of CD8+ T cell bivariate model (Spearman correlation, Rs =0.75, FDR =8.24 × 10)^-4) Two-variable model equation 0.31 × TCR diversity + 8.87 × 13 CD8⁺T Cells + 0.27. LUAD, lung adenocarcinoma; SKCM, cutaneous melanoma; PRAD, prostate cancer; BLCA, bladder urothelial cancer; MESO, mesothelioma; BRCA, breast invasive carcinoma; CESC, cervical squamous carcinoma and cervical adenocarcinoma; PAAD, pancreatic cancer; OV, ovarian serous cystadenocarcinoma; HNSC, head and neck squamous cell carcinoma; STAD, gastric adenocarcinoma; THCA, thyroid cancer; CHOL, cholangiocarcinoma; ACC, adrenocortical carcinoma; READ, rectal adenocarcinoma; COAD, colon cancer; LIHC, hepatocellular carcinoma; LGG, brain low-grade glioma; GBM, glioblastoma multiforme; UVM, uveal melanoma; UCS, uterine carcinosarcoma;

FIG. 2 is a graph showing the comprehensive identification of potential predictive markers of irae.A. pathway enrichment analysis was performed on the first 10 genes with the most significant irAE ROR correlations for multiple cancer types.B. relevance line Spearman test for LCP1 and irAE ROR.C. bivariate predictive model analysis was performed on the first 10 genes with significant irAE ROR.Spearman correlation (Rs) test was performed on predicted and observed irAE ROR.color shade of squares indicates the magnitude of Rs, the magnitude indicates the significant difference of log likelihood test.d.LCP1 and ADPGK combination was subjected to two-factor model evaluation (Spearman correlation, Rs =0.91, FDR =7.94 × 10)^-9) The two-variable regression model formula 0.37 × LCP1+ 0.70 × ADPGK-9.10.

FIG. 3 is a graph showing the predictive ability of LCP1 and ADPGK validated in an independent patient cohort A. ADPGK and LCP1 immunohistochemical staining pictures of patients with and without irAE A Picture size: 200 × 200 μm². b. LCP and ADPGK immunohistochemical staining signals were quantified. Unpaired two-tailed student's t test was performed between immunohistochemical staining signals from patients who developed irAE and those who did not. c. The geometric mean of the LCP1 and ADPGK staining signals was calculated. The unpaired two-tailed student's t test was performed on patients who developed irAE and on patients who did not develop irAE. d. ROC curves for LCP1, ADPGK, LCP1+ ADPGK are shown for this patient cohort (n = 28).

FIG. 4 is a graph showing the Spearman correlation test for iriAE ROR and objective remission rate in 21 cancer types.

Fig. 5 is a graph showing irAE ROR and a cytolytic activity, B. interferon γ signature, c.pd-1 expression, d.tcr diversity, e. estimated M1 macrophage abundance, f. estimated CD8+ T cell abundance, g. estimated naive B cell abundance, in patients treated with anti-PD-1/PD-L1 in each cancer type. The color of the dots represents different tumor types.

Fig. 6 is a graph showing the predicted effect of a bivariate model calculating all combinations of 7 most significant correlation factors. The results only show combinations of significant p-values for the log-likelihood test.

Fig. 7 is a graph showing variance dilation factor (VIF) showing 7 most significant correlation factors with irAEROR.

FIG. 8 is a graph showing a Spearman correlation test for iraE ROR and the top 10 significantly related genes (except LCP 1). The X-axis represents the expression level of the gene transformed with log 2.

Fig. 9 is a graph showing the predicted effect of two-by-two combinations of the first 10 genes associated with the bivariate model evaluation and irAE ROR. The results only show combinations of significant p-values for the log-likelihood test.

Fig. 10 is a graph showing Variance Inflation Factor (VIF) demonstrating the top 10 significantly related genes.

Fig. 11 is a diagram showing the testing of the predictive model by the independent patient cohort. a. LCP1, ADPGK, LCP1+ ADPGK gene expression in brain regions with and without irAE. b. LCP1, ADPGK, LCP1+ ADPGK gene expression in the presence of irAE heart muscle and in the absence of irAE smooth muscle. The calculation of LCP1+ ADPGK model scores was built on the LCP1+ ADPGK two-variable model (fig. 2 d). TPM: number of transcripts per million kilobases.

FIG. 12 is a graph showing two-variable model detection of the combination of the first 7 factors and the first 10 genes of iraE. Spearman r (rs) calculated the correlation between prediction and observed irAE ROR. The squares are light and dark in color to indicate Rs and large in size to indicate the significance of the log-likelihood ratio test. b. The two-factor model calculated the combined effect of CD8+ T cells and LCP1 (Spearman corporation, Rs =0.87, FDR =2.84 × 10-72).

FIG. 13 is a graph showing the predictive effect of the bivariate model evaluating the first 7 factors and the first 10 related genes in combinations of two. The results only show combinations of significant p-values for the log-likelihood test.

FIG. 14 is a graph showing the objective remission rate against PD-1/PD-L1 and LCP1, ADPGK expression for a Spearman correlation test in 21 tumor types.

Figure 15 is a graph showing the predictive ability to test LCP1 and ADPGK in a lung tumor patient cohort. a. Lung cancer patients were quantitatively analyzed for ADPGK, LCP1 immunohistochemical staining signals. Unpaired two-tailed t-tests were performed between the groups with and without irAE. b. The geometric mean of the staining signals for ADPGK, LCP1 was calculated. Unpaired two-tailed t-tests were performed between the groups with and without irAE. c. LCP1, ADPGK, LCP1+ ADPGK ROC curve (n = 26) of this patient cohort.

Detailed Description

In order to further understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products, and all of them are commercially available.

Example 1

1 method of experiment

(1.1) data analysis of FAERS personal safety reports

The invention relates to FAERS (https:// www.fda.gov/drugs/queries-and-answers-fdas-overture-event-

reporting-system-faces/fda-added-event-reporting-system-faces-public-dashboard) obtains personal security reports from 7/1/2014 to 6/30/2019. Adverse reaction reports were collected only for patients receiving anti-PD-1 antibodies (nivolumitumumab, pembrolizumab, cimetizumab) and anti-PD-L1 (alemtuzumab, avizumab, doxumab) and excluded patients (leprimumab, tremelimumab) who had received anti-CTLA-4 antibody treatment at the same time. The irAE was defined using the peer approved irAE administration manual as a standard. And (4) adopting an imbalance analysis, and calculating ROR of the risk occurrence of iraE by using the whole database as a comparison sample. Patients were classified into the irAE group if any of the irAE types were produced.

(1.2) analysis of TCGA database and independent database

Molecular data including mRNA expression, miRNA expression, protein expression, somatic mutations were obtained for 26 cancer types from the TCGA database (https:// portal.gdc.candr.gov /). TCR diversity, neoantigen load, predicted immune cell abundance, and intra-tumor heterogeneity data were obtained from GDC PanImmune data (https:// GDC. cancer. gov/aboutdata/publications-/panimmun). TMB counts the number of non-silent somatic mutations per sample. The "GSVA" R package was used to calculate T-cell inflammatory Gene Expression (GEP) for each sample, with the T-cell inflammatory GEP signature and interferon gamma signature defined by the eyers et al. The cytolytic activity is defined as the geometric mean of the expression levels of two cytolytic marker genes (GZMA, PRF 1). RNA-seq data was obtained from Johnson et al for brain areas affected/unaffected by iraE, and for heart muscles affected by iraE and unaffected smooth muscles.

(1.3) identification of biomarkers combining multiple sets of mathematical data and real world data

In the analysis of the present invention, the number of cancer types was much lower than the number of variables (26 cancer types)>The 50,000 variables, including 20,000 mRNA expression, 12,000 non-coding RNA expression, 18,000 gene mutation, 200 protein expression, and 2,400miRNA expression) may lead to the first class of errors, causing more false positives when using other advanced algorithms, such as Lasso, Elastic net, Ridge. Therefore, the invention optimizes the calculation result by adding variables. Median values for each factor in each cancer type were calculated. The human anatomy diagrams were plotted using the "gganatogram" R-package and the irAE ROR was predicted in two-and three-variable linear regression models using the Cross-Validation test (Leave-One-Out Cross Validation) method provided by the "caret" R-package (see fig. 8). Using Spearman rank correlation coefficient (Rs) and unexplained coefficient of variation (1-Rs)²) And evaluating the prediction result. The goodness of fit of the models was compared using the log-likelihood ratio test in the R package "lmtest". And carrying out log likelihood ratio test on the two-factor model and the single factor with higher Rs in the two factors to obtain data between the fitness of the two-variable model. For the examination of the three-factor fitness, a row log-likelihood ratio test between a two-factor model and a three-factor model is adopted. The multiple collinearity was evaluated using the 'vif' function in the R package 'car' to calculate the coefficient of variance expansion. The pathway enrichment was calculated using the R package "clusterprofiler". The area under the ROC curve was calculated using R package 'pROC'. Significant difference was defined as two-tailed P<0.05 and/or FDR<0.05。

(1.4) immunohistochemistry

The study of the invention conforms to the ethical guidelines of the protective general guidelines of the U.S. subjects and is approved by the ethical committee of the general hospital of the chinese liberation force. All patients signed informed consent. All patients received treatment at the general hospital of the chinese liberation force and were analyzed retrospectively for clinical information and tissue samples. Formalin-fixed paraffin embedding (FFPE) was obtained in pathological examination. FFPE tissue sections were 5 μm thick and immunohistochemical examination was performed. The primary antibody was either LCP1 (1: 200, Cell Signaling Technology #3588) or ADPGK (1:900, Novus Biologicals # NBP 1-91653). After washing the primary antibody, horseradish peroxidase-labeled secondary antibodies were incubated, followed by color development using a DAB horseradish peroxidase color development kit (Dako). The section is counterstained by hematoxylin, dehydrated and loaded with broken sections and sealed. The whole piece was scanned using an Aperio ScanScope system (Leica Biosystems) and the staining results were quantified using the positive pixel count v9 (PPCv9) algorithm provided by Aperio ImageScope software v 14.3. Necrotic areas or image defects are ignored. 7 random fields of view of 20 times were selected for each section to count the average color signal of the section.

2. Results and discussion

(2.1) analysis of the role of known factors in predicting irAEs

To identify potential biomarkers for anti-PD-1/PD-L1 antibody treatment of irAEs, the present invention obtained 18,706 patients receiving anti-PD-1/PD-L1 antibody treatment from the american Food and Drug Administration (FDA) adverse event reporting system (FAERS), covering 26 cancer types, 52,282 adverse reaction (AEs) events. Of these, 3,706 (19.8%) patients developed at least one irAEs. The present invention calculates the proportion of irAEs reported by anti-PD-1/PD-L1 antibody treatment and the proportion of irAEs reported by other drugs in the database, and calculates the Reporting Odds Ratio (ROR) of irAEs caused by anti-PD-1/PD-L1 antibody treatment. IrAE ROR varies between different cancer types and has the highest value for lung adenocarcinoma (LUAD) (3.29, 95% confidence interval [ CL)]2.97-3.65), uterine sarcoma (UCS) was the lowest value (0.65, 95% CL, 0.02-4.18) (FIG. 1 a.) the present invention analyzed 6 iraE-related factors, including TMB, T cell receptor diversity, interferon gamma expression, tumor necrosis factor α expression, eosinophils and neutrophils surprisingly, these factors showed a positive correlation in iraE incidence and benefit in patients receiving immune checkpoint inhibitors, and therefore were also biomarkers of immune therapeutic response, iraE R treated with anti-PD-1/PD-L1 antibodies was also observedThere was only a slight significant correlation between OR and Objective Remission Rate (ORR) (Rs =0.44; P =0.049; fig. 4). Next, 36 factors associated with immunotherapy response were examined, including TMB, cytolytic activity, neoantigen loading. Molecular data for these factors were obtained from The tumor Genome project (TCGA) database, irAE risk reports were obtained from FAERS, and correlations between molecular data for each factor and irAE risk index were calculated. 7 potential predictors were found, including cytolytic activity (Spearman R, Rs =0.64; False discovery rate [ FDR ]]= 0.01), interferon gamma characteristics (Rs =0.61, FDR = 0.01), PD-1 expression (Rs =0.60, FDR = 0.01), TCR diversity (Rs =0.59, FDR = 0.01), M1 type macrophages (Rs =0.55, FDR = 0.03), CD8+ T cell abundance (Rs =0.50, FDR = 0.05), naive B cells (Rs =0.49, FDR = 0.05) (fig. 1B; fig. 5). to obtain a more accurate prediction model, a two-variable model was evaluated on the 7-factor Spearman correlation coefficient and log-likelihood ratio fitness test value (log-likehood ratio test) in combination of CD8+ T cell abundance and TCR diversity or naive B cell factors, and using a single factor, the model's fit could be significantly improved compared to using a single factor (fig. 1c; fig. 6 and 7.8. in particular, TCR diversity could be combined with TCR 2, TCR abundance ((TCR) 3.8) and TCR diversity) when the prediction level was taken into account^-4) (FIG. 1 d). The correlation coefficient (Rs, 0.75) accounts for 56% of the observed iraE ROR (Rs)²0.56) can be interpreted using the two-variable regression model. Multiple collinearity of these 7 factors was evaluated using Variance Inflationfactor (VIF), with no multiple collinearity observed in TCR diversity and CD8+ T cells (fig. 7). Meanwhile, no significant correlation was found between TCR diversity and CD8+ T cell abundance (P = 0.26), indicating that the two are independent in predicting irAE. The prediction effect of other factors combined with a TCR diversity-CD 8+ T cell abundance bivariate model is further evaluated, and no trivariate model is found to be helpful for improving the correlation coefficient or increasing the accuracy.

(2.2) comprehensive identification of potential biomarkers for irAE

The top predictor is mostly genes with altered expression and is highly enriched in the course of the immune response, including T cell activation and cell killing (fig. 2 a). this result further confirms that T cells are key regulators of irAEs, surprisingly, lymphocyte plasma-encapsulating protein 1 (LCP 1) involved in T cell activation has the highest level of correlation coefficient (Rs =0.82, FDR = 156.69 × 10)^-3Figure 2 b) further two-variable model evaluation of the top ten irAE-related genes showed substantially better predictive effect of LCP1 combined with most other irAE-related genes (figures 2c and 9). when combined with adenosine diphosphate dependent glucokinase (ADPGK) which induces metabolic switching in T cell activation, and LCP1, linear regression analysis of all two-variable models resulted in the best accuracy (Rs =0.91, FDR =7.94 10-9, figure 2 d) also used with multiple f to evaluate the co-linearity of the top 10 genes, no multiple co-linearity was observed in LCP1 and ADPGK (figure 10), no increase in 3 gene to LPC 1-adpk two-variable model predictive value was observed for the non-occurring region/tissue (figure 10), no further significant increase in LCP 1-adpk two-variable model was observed, and no further significant response was observed in the combined evaluation of LCP1, and no further significant response was observed to the results of the combined evaluation of LCP 3578, and no further significant response was found to the clinical findings of the results of the combined evaluation of the results of the clinically significant response of LCP 355636 and pgk (figure 11).

(2.3) verification that LCP1 and ADPGK are irAE biomarkers

To examine the predictive power of LCP1 and ADPGK, a validated cohort of 28 patients receiving anti-PD-1/PD-L1 inhibitor treatment with high quality Formaldehyde Fixed Paraffin Embedded (FFPE) tissue sections and clinical pathology information was collected. The median age of the patients was 56 years (range, 37 to 82 years), of which 22 (78.6%) were male patients and 6 (21.4%) were female patients. 26/28 (92.9%) patients diagnosed with lung cancer. Immunohistochemistry was used to examine the levels of LCP1 and ADPGK expression in patients in the validation cohort. LCP1 and ADPGK stained significantly more in the irAE group (fig. 3 a). Immunostaining signals for LCP1 and ADPGK were quantified using the Positive Pixel Count v9 (PPCv9) algorithm of Aperio ImageScope software (v 14.3). As a result, as expected, the expression levels of LCP1 ((p-value = 0.008) and ADPGK (p-value = 0.010) in patients with an irAE were significantly higher than the expression levels in patients without an irAE (FIG. 3 b). the geometric mean values of LCP1 and ADPGK expression were also significantly higher in patients with an irAE ((p-value = 0.005, FIG. 3 c). LCP1 and ADPGK predict the receiver operating characteristic curve (ROC) of an irAE, the area under the curve (AUC) was 0.78 and 0.78, respectively, and a better AUC area was obtained when LCP1 and ADPGK were combined (0.8, FIG. 3 d). furthermore, LCP1, ADPGK, 1+ ADPGK successfully predicted the occurrence of localized pneumonia in 26 patients with lung cancer, the AUC was 0.74, 0.76 and 0.77, respectively, and the LCP 6777 was shown by a comprehensive model of a PGK inhibitor of a type that was able to predict the occurrence of an IRAE in a patient with an IRAE and a tumor, and a combined type of an ADPGK 6778, and a PGK-7, and a combined type of an ADPGK-7, and a patient were able to predict the incidence of an IRAE, and a tumor Shengirae.

(2.4) conclusion

In the study of the present invention, real world and molecular data from patients receiving anti-PD-1/PD-L1 therapy were integrated in 26 tumor types and systematically analyzed for potential predictors of risk of developing irAE. 7 potential predictors were identified, the CD8+ T cell and TCR diversity combinations achieved the best prediction accuracy for iraE, with an unexplained coefficient of variation from 0.59 (1-0.64)²) Down to 0.44 (1-0.75)²). Taking account of the unexplained coefficient of variationHowever at 0.44, a large-scale comprehensive screen was therefore performed to obtain better prediction factors for irAE ROR. New possible irAE predictors were found to be enriched during T cell activation. The combination of the two genes related to T cell activation, LCP1 and ADPGK, evaluated in a linear regression model, gave an unexplained coefficient of variation from 0.44 ((1-0.75)²) Reduced to 0.17 (1-0.91)²). Furthermore, AUC values for LCP1 and ADPGK reached 0.8 in the patient level validation cohort, indicating that LCP1 and ADPGK in combination are biomarkers predictive of irAE occurrence.

The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations of the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.

Claims

1. Use of an immune-related adverse reaction biomarker comprising LCP1 and/or ADPGK in the preparation of a kit for predicting an immune-related adverse reaction.

2. Use of a pre-test kit for immune-related adverse reactions comprising an agent for detecting the expression level of LCP1 and/or ADPGK in the manufacture of a kit for predicting immune-related adverse reactions.

3. The use according to claim 1 or 2, wherein the immune-related side effects are immune-related side effects caused by anti-PD-1/PD-L1 antibody treatment.

4. The use of claim 3, wherein the anti-PD-1 antibody is selected from the group consisting of one or more of nivolumab, pembrolizumab, and cimicizumab; the anti-PD-L1 is selected from one or more of atelizumab, avizumab and Duvacizumab.

5. The use of claim 4, wherein the subject treated with the anti-PD-1/PD-L1 antibody includes, but is not limited to, lung adenocarcinoma; cutaneous melanoma; prostate cancer; bladder urothelial cancer; mesothelioma; invasive carcinoma of the breast; squamous carcinoma of the cervix and adenocarcinoma of the cervix; pancreatic cancer; ovarian serous cystadenocarcinoma; squamous cell carcinoma of the head and neck; gastric adenocarcinoma; thyroid cancer; bile duct cancer; adrenocortical carcinoma; rectal adenocarcinoma; colon cancer; hepatocellular carcinoma; brain low-grade glioma; glioblastoma multiforme; uveal melanoma; patients with uterine carcinosarcoma.