CN111733063A - Microbial agent for improving utilization of nitrogen in saline-alkali soil, application method and preparation device - Google Patents
Microbial agent for improving utilization of nitrogen in saline-alkali soil, application method and preparation device Download PDFInfo
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- CN111733063A CN111733063A CN202010741668.7A CN202010741668A CN111733063A CN 111733063 A CN111733063 A CN 111733063A CN 202010741668 A CN202010741668 A CN 202010741668A CN 111733063 A CN111733063 A CN 111733063A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention relates to the technical field of microbial agents and soil nitrogen nutrient conversion, in particular to a microbial agent for improving the utilization of saline-alkali soil nitrogen, an application method and a preparation device. The invention can realize the full mixing of the raw materials and the nutrient raw materials before the preparation, and ensure the supplement of sufficient carbon and nitrogen nutrition in the subsequent culture of microorganisms.
Description
Technical Field
The invention relates to the technical field of microbial agents and utilization efficiency of soil nutrients, in particular to a microbial agent for improving utilization of saline-alkali soil nitrogen, an application method and a preparation device.
Background
The microbial fertilizer contains various efficient active beneficial microbial bacteria, is suitable for planting various soils and crops, increases the number and activity of the beneficial microbial bacteria in the soil, can activate soil nutrients, improves the utilization rate of the soil nutrients, enhances the disease resistance of the crops, prevents pathogenic bacteria from invading, promotes the growth of the crops and improves the yield of the crops.
The microbial preparation device on the existing market mostly takes broad-spectrum microbial strains as the main part, and simultaneously has no working procedure of mixing preparation raw materials of various microbial agents, so that the microbial survival rate in the microbial agents is low, the soil nitrogen conversion efficiency is low, the nitrogen loss is high, and the chemical nitrogen fertilizer utilization rate is not high when the microbes are prepared in the later period.
Disclosure of Invention
The invention aims to solve the problems that the microbial preparation raw materials for promoting the soil nitrogen conversion rate are not uniformly mixed and the microbes cannot uniformly obtain sufficient nutrients in the prior art, so that the microbial survival rate is low, the utilization rate of chemical fertilizers, particularly nitrogen fertilizers is low and the like in the later preparation of the microbes. In order to achieve the purpose, the invention adopts the following technical scheme:
the device for preparing the microbial agent for improving the utilization of the saline-alkali soil nitrogen comprises a base and a shell, wherein the shell is positioned above the base, the upper end of the shell is provided with an opening, the middle part of the upper end of the base is fixedly provided with a rotating mechanism and is connected with the middle part of the lower end of the shell through the rotating mechanism, the upper ends of the two sides in the shell are fixedly provided with clamping mechanisms, a containing box is fixed through two groups of clamping mechanisms, a gap is arranged between the lower end of the containing box and the lower end of the inner side of the shell, one side of the upper end of the base is vertically welded with a motor support, one side of the motor support is fixedly provided with a stepping motor through a screw, an output shaft of the stepping motor penetrates through the motor support and extends to the other side of the motor support, the tail end of the output shaft of the stepping motor is fixedly provided, and auxiliary supporting mechanisms are arranged between the two sides of the upper end of the base and the two sides of the lower end of the shell.
Preferably, slewing mechanism includes the fixing base of fixed mounting at shell lower extreme middle part and the bracing piece of fixed mounting in the base upper end, the upper end welding of bracing piece has the spheroid, ball type groove has been seted up to the inboard lower extreme of fixing base, the spheroid rotates the inboard that sets up in ball type groove, and the size in ball type groove matches each other with spheroidal size, spheroidal diameter is greater than the diameter size of ball type groove lower extreme opening part.
Preferably, block mechanism includes that fixed mounting is at the fixture block that holds box one side middle part and sets up the square groove in the inside one side upper end of shell, the fixture block card is established in the inboard of square groove, and is interference fit between square groove and the fixture block, two reference columns are vertically installed to the bottom of square groove, and the upper end of two reference columns runs through the fixture block and extends to the top of fixture block.
Preferably, drive mechanism includes the cylinder of fixed mounting in the outside one side of disc and installs the spacing post in shell one side upper end, the outside cover of spacing post is equipped with the second ring, the outside cover of cylinder is equipped with first ring, be connected with the connecting rod between second ring and the first ring.
Preferably, one end of the outer side of the limiting column is in threaded connection with a limiting block, and the diameter of the limiting block is larger than that of the limiting column.
Preferably, the auxiliary supporting mechanism comprises connecting rings fixedly installed on one side of the upper end of the base and one side of the lower end of the shell, and a return spring is fixedly connected between the two connecting rings.
Preferably, a rubber pad is compositely bonded on the lower end face of the base.
The invention also provides an application method of the microbial agent for improving the utilization of the nitrogen in the saline-alkali soil, which comprises the following specific steps:
s1, fixedly mounting the containing box on the inner side of the shell through a clamping mechanism, pouring more than ten kinds of microbial agent raw materials mainly comprising saline-alkali resistant azotobacter, nitrobacteria, nitrite bacteria, ammonia oxidizing bacteria and ammonia oxidizing archaea microbial strains into the containing box, and turning on a working power supply of the stepping motor;
s2, the height of the upper end of the microbial agent raw material should not exceed three quarters of the height of the inner side of the containing box, and meanwhile, the material mixing process can be stopped after the raw materials are mixed for 5 minutes, the working power supply of the stepping motor is cut off, and the containing box is detached from the inside of the shell.
The invention also provides a microbial agent for improving the utilization of the nitrogen in the saline-alkali soil, and the specific formula is as follows: 50-55 parts of microbial inoculum, 30-45 parts of protein, 19-22 parts of vitamin, 22-25 parts of mineral matter, 50-55 parts of carbon nutrition, 2-3 parts of nitrogen nutrition, 8-10 parts of dietary fiber and 12-15 parts of fatty acid.
The invention provides a preparation device and an application method of a microbial agent for improving the utilization of nitrogen in saline-alkali soil, which have the beneficial effects that: the microbial agent preparation device for improving the nitrogen content of the saline-alkali soil and the application method thereof are formed by mixing more than ten microbial agents mainly comprising saline-alkali resistant azotobacter, nitrobacteria, nitrite bacteria, ammonia oxidizing bacteria and ammonia oxidizing archaea microbial strains, and meanwhile, the microbial agent contains various components such as carbon nitrogen nutrients and mineral elements required by microbes, can fully mix nutrient raw materials before preparation, and ensures that sufficient nutrition is supplemented in the subsequent culture of the microbes.
Drawings
FIG. 1 is a structural front view of a microbial agent preparation device and an application method for improving the utilization of nitrogen in saline-alkali soil according to the present invention.
FIG. 2 is a top view of the structure of the microbial preparation device and the application method for improving the nitrogen utilization of saline-alkali soil.
FIG. 3 is an enlarged view of the structure of part A of the microbial agent preparation apparatus and application method for enhancing the utilization of nitrogen in saline-alkali soil as set forth in FIG. 1.
FIG. 4 is an enlarged view of the structure of part B of the apparatus for preparing a microbial agent for enhancing the utilization of nitrogen in saline-alkali soil and the application method thereof, as suggested in FIG. 2.
In the figure: the device comprises a motor support 1, a cylinder 2, a rotating mechanism 3, a fixed seat 31, a spherical groove 32, a sphere 33, a support rod 34, a clamping mechanism 4, a square groove 41, a clamping block 42, a positioning column 43, a disc 5, a connecting rod 6, a first circular ring 7, a containing box 8, a return spring 9, a connecting ring 10, a base 11, a rubber pad 12, a shell 13, a limiting block 14, a second circular ring 15, a limiting column 16 and a stepping motor 17.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1
Referring to fig. 1-2, the preparation device of the microbial agent for improving the utilization of the nitrogen in the saline-alkali soil comprises a base 11 and a shell 13, wherein a rubber pad 12 is compositely bonded on the lower end surface of the base 11, and the rubber pad 12 can play a role in buffering and damping and improving the stability.
The shell 13 is located the top of base 11, and the upper end of shell 13 is the opening setting, holds and is provided with the gap between the inboard lower extreme of the lower extreme of box 8 and shell 13, and the vertical welding in upper end one side of base 11 has motor support 1, and screw fixed mounting has step motor 17 in one side of motor support 1, and step motor 17 is connected with power supply unit through the wire, and is external to have control switch on the wire.
An output shaft of a stepping motor 17 penetrates through the motor support 1 and extends to the other side of the motor support 1, a disc 5 is fixedly mounted at the tail end of the output shaft of the stepping motor 17, a transmission mechanism is arranged between the outer side of the disc 5 and the upper end of one side of the outer part of the containing box 8, the transmission mechanism comprises a cylinder 2 fixedly mounted on one side of the outer part of the disc 5 and a limiting column 16 mounted on the upper end of one side of the shell 13, a second circular ring 15 is sleeved on the outer side of the limiting column 16, a first circular ring 7 is sleeved on the outer side of the cylinder 2, a connecting rod 6 is connected between the second circular ring 15 and the first circular ring 7, a limiting block 14 is in threaded connection with one end of the outer side of the limiting column 16, the diameter of the limiting block 14 is larger than that of the limiting column 16, after a working power supply of the stepping motor 17 is, simultaneously, the bottom of the connecting rod 6 transmits power to the limiting column 16 through the second circular ring 15, so that the limiting column 16 drives one side of the shell 13 to swing up and down, raw materials of the microbial bacteria and the nutrient can be fully mixed together, and the survival rate of later-stage strain cultivation is improved.
All be provided with auxiliary stay mechanism between the upper end both sides of base 11 and the lower extreme both sides of shell 13, auxiliary stay mechanism includes the go-between 10 of fixed mounting in base 11 upper end one side and shell 13 lower extreme one side, fixedly connected with reset spring 9 between two go-between 10, when the both sides luffing motion of shell 13, two reset spring 9 are in shrink and tensile two kinds of states respectively, play support and spacing effect of removal to shell 13, improve shell 13 wobbling stability.
The invention also provides an application method of the microbial agent for improving the utilization of the nitrogen in the saline-alkali soil, which comprises the following specific steps:
s1, fixedly installing the containing box 8 on the inner side of the shell 13 through the clamping mechanism 4, pouring the microbial agent raw material into the containing box 8, and turning on the working power supply of the stepping motor 17;
s2, the height of the upper end of the raw material of the microbial inoculum should not exceed three quarters of the height of the inner side of the containing box 8, and the mixing process can be stopped after the raw materials are mixed for 5 minutes, the working power supply of the stepping motor 17 is cut off, and the containing box 8 is detached from the inside of the shell 13.
The invention also provides a microbial agent for improving the utilization of the nitrogen in the saline-alkali soil, and the specific formula is as follows: 43 parts of microbial inoculum, 52 parts of protein, 21 parts of vitamin, 23 parts of mineral substances, 9 parts of dietary fiber and 13 parts of fatty acid.
Example 2
Referring to fig. 3, the difference between this embodiment and embodiment 1 is that a rotating mechanism 3 is fixedly installed in the middle of the upper end of a base 11, and is connected to the middle of the lower end of a housing 13 through the rotating mechanism 3, the rotating mechanism 3 includes a fixed seat 31 fixedly installed in the middle of the lower end of the housing 13 and a support rod 34 fixedly installed in the upper end of the base 11, a ball 33 is welded to the upper end of the support rod 34, a ball groove 32 is formed in the lower end of the inner side of the fixed seat 31, the ball 33 is rotatably disposed in the inner side of the ball groove 32, the size of the ball groove 32 matches the size of the ball 33, the diameter of the ball 33 is greater than the diameter of the opening at the lower end of the ball groove 32, when the housing 13 swings on both sides, the ball 33 rotates in the ball groove 33, and the diameter of the ball 33 is greater than the diameter of, so that the rotation of the housing 13 is smoother.
Example 3
Referring to fig. 4, the difference between this embodiment and embodiments 1 and 2 lies in that the upper ends of two sides of the inside of the housing 13 are fixedly installed with the engaging mechanisms 4, and the containing box 8 is fixed by two sets of engaging mechanisms 4, the engaging mechanism 4 includes a fixture block 42 fixedly installed in the middle of one side of the containing box 8 and a square groove 41 opened in the upper end of one side of the inside of the housing 13, the fixture block 42 is clamped inside the square groove 41, and the square groove 41 and the fixture block 42 are in interference fit, two positioning posts 43 are vertically installed at the bottom of the square groove 41, and the upper ends of the two positioning posts 43 penetrate through the fixture block 42 and extend to the upper side of the fixture block 42, the fixture block 42 is clamped in the square groove 41, so that the containing box 8 can be stably clamped at the upper end of the inside of the housing 13, and the positioning posts 43 can further play a positioning role, the situation that the cartridge 8 is shaken inside the housing 13 is prevented.
The invention also provides a microbial agent for improving the utilization of the nitrogen in the saline-alkali soil, and the specific formula is as follows: 43 parts of microbial inoculum, 52 parts of protein, 21 parts of vitamin, 23 parts of mineral substances, 9 parts of dietary fiber, 40 parts of carbon nutrient components, 2 parts of nitrogen nutrient components and 13 parts of fatty acid.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. Improve microbial inoculum's of saline-alkali soil nitrogen utilization preparation facilities, including base (11) and shell (13), shell (13) are located the top of base (11), and the upper end of shell (13) is the opening setting, its characterized in that, the upper end middle part fixed mounting of base (11) has slewing mechanism (3), and realizes being connected through slewing mechanism (3) and the lower extreme middle part of shell (13), the equal fixed mounting in inside both sides upper end of shell (13) has block mechanism (4), and is fixed with through two sets of block mechanism (4) and holds box (8), it is provided with the gap to hold between the lower extreme of box (8) and the inboard lower extreme of shell (13), the vertical welding in upper end one side of base (11) has motor support (1), one side of motor support (1) has step motor (17) through screw fixed mounting, the output shaft of step motor (17) runs through motor support (1) and extends to the opposite side of motor support (1), the terminal fixed mounting of output shaft of step motor (17) has disc (5), be provided with drive mechanism between the outside of disc (5) and the outside one side upper end that holds box (8), all be provided with auxiliary stay mechanism between the upper end both sides of base (11) and the lower extreme both sides of shell (13).
2. The preparation device of the microbial inoculant for improving nitrogen utilization of saline-alkali soil as claimed in claim 1, wherein the rotating mechanism (3) comprises a fixed seat (31) fixedly installed in the middle of the lower end of the housing (13) and a supporting rod (34) fixedly installed on the upper end of the base (11), a sphere (33) is welded on the upper end of the supporting rod (34), a spherical groove (32) is formed in the lower end of the inner side of the fixed seat (31), the sphere (33) is rotatably arranged on the inner side of the spherical groove (32), the size of the spherical groove (32) is matched with that of the sphere (33), and the diameter of the sphere (33) is larger than that of the opening at the lower end of the spherical groove (32).
3. The apparatus for preparing a microbial inoculant for improving nitrogen utilization of saline-alkali soil according to claim 1, wherein the clamping mechanism (4) comprises a clamping block (42) fixedly installed in the middle of one side of the containing box (8) and a square groove (41) formed in the upper end of one side inside the housing (13), the clamping block (42) is clamped inside the square groove (41), the square groove (41) and the clamping block (42) are in interference fit, two positioning columns (43) are vertically installed at the bottom of the square groove (41), and the upper ends of the two positioning columns (43) penetrate through the clamping block (42) and extend to the upper side of the clamping block (42).
4. The apparatus for preparing the microbial inoculum for improving the nitrogen utilization of saline-alkali soil as claimed in claim 1, wherein the transmission mechanism comprises a cylinder (2) fixedly installed on one side of the outside of the disc (5) and a limit column (16) installed on the upper end of one side of the housing (13), the outside of the limit column (16) is sleeved with a second ring (15), the outside of the cylinder (2) is sleeved with a first ring (7), and a connecting rod (6) is connected between the second ring (15) and the first ring (7).
5. The preparation device of the microbial inoculum for improving the nitrogen utilization of the saline-alkali soil as claimed in claim 4, wherein one end of the outer side of the limiting column (16) is in threaded connection with a limiting block (14), and the diameter of the limiting block (14) is larger than the diameter of the limiting column (16).
6. The apparatus for preparing microbial inoculum for improving nitrogen utilization of saline-alkali soil as claimed in claim 1, wherein the auxiliary supporting mechanism comprises connecting rings (10) fixedly installed on one side of the upper end of the base (11) and one side of the lower end of the housing (13), and a return spring (9) is fixedly connected between the two connecting rings (10).
7. The apparatus for preparing microbial inoculum for improving nitrogen utilization of saline-alkali soil as claimed in claim 1, wherein the lower end surface of the base (11) is compositely adhered with a rubber pad (12).
8. The application method of the microbial agent for improving the nitrogen utilization of the saline-alkali soil as claimed in claims 1 to 7, which is characterized by comprising the following specific steps of:
s1, fixedly installing the containing box (8) on the inner side of the shell (13) through the clamping mechanism (4), pouring the microbial agent raw material into the containing box (8), and turning on the working power supply of the stepping motor (17);
s2, the height of the upper end of the microbial agent raw material should not exceed three-quarters of the height of the inner side of the containing box (8), the material mixing process can be stopped after the raw materials are mixed for 5 minutes, the working power supply of the stepping motor (17) is cut off, and the containing box (8) is detached from the inner part of the shell (13).
9. The microbial agent for improving the nitrogen utilization of the saline-alkali soil according to claims 1 to 8, which is characterized by comprising the following specific formula: 40-45 parts of microbial inoculum, 50-55 parts of protein, 19-22 parts of vitamin, 22-25 parts of mineral, 8-10 parts of dietary fiber and 12-15 parts of fatty acid.
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Citations (4)
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CN107347770A (en) * | 2017-08-14 | 2017-11-17 | 侯添有 | A kind of fishery cultivating wind spraying aid type charging device |
US10434551B1 (en) * | 2018-12-10 | 2019-10-08 | China University Of Petroleum (East China) | Method for improving saline-alkaline soil by using biomass rapid pyrolysis product |
CN210314082U (en) * | 2019-04-25 | 2020-04-14 | 南京川博生物技术有限公司 | Myoblast determinant gene 1 total protein antibody rapid extraction device |
CN210925267U (en) * | 2019-11-27 | 2020-07-03 | 盐城盐龙文化体育产业投资有限公司 | A multimedia culture propagates device for garden |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107347770A (en) * | 2017-08-14 | 2017-11-17 | 侯添有 | A kind of fishery cultivating wind spraying aid type charging device |
US10434551B1 (en) * | 2018-12-10 | 2019-10-08 | China University Of Petroleum (East China) | Method for improving saline-alkaline soil by using biomass rapid pyrolysis product |
CN210314082U (en) * | 2019-04-25 | 2020-04-14 | 南京川博生物技术有限公司 | Myoblast determinant gene 1 total protein antibody rapid extraction device |
CN210925267U (en) * | 2019-11-27 | 2020-07-03 | 盐城盐龙文化体育产业投资有限公司 | A multimedia culture propagates device for garden |
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