CN111728973A - Medicine for resisting novel coronavirus SARS-CoV-2 and its application - Google Patents
Medicine for resisting novel coronavirus SARS-CoV-2 and its application Download PDFInfo
- Publication number
- CN111728973A CN111728973A CN202010461381.9A CN202010461381A CN111728973A CN 111728973 A CN111728973 A CN 111728973A CN 202010461381 A CN202010461381 A CN 202010461381A CN 111728973 A CN111728973 A CN 111728973A
- Authority
- CN
- China
- Prior art keywords
- hcl
- cov
- sars
- pharmaceutical composition
- coronavirus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pulmonology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a series of medicines for resisting novel coronavirus SARS-CoV-2 and application thereof, and in vitro cell test results show that: the compound can obviously inhibit the infection of SARS-CoV-2 to normal cells, has an inhibiting effect on novel coronavirus SARS-CoV-2 at a cell level, can obviously reduce the virus price of the virus in the cells, and inhibits the proliferation of the virus in the cells, and has dose dependence. Therefore, the compound can be used as a novel coronavirus SARS-CoV-2 inhibitor, and has the potential of treating COVID-19 pneumonia caused by human infection with the virus or other diseases caused by animal infection.
Description
Technical Field
The invention belongs to the field of virology and pharmaceutical chemistry, and particularly relates to a compound for inhibiting the replication of a novel coronavirus SARS-CoV-2 virus, a mechanism for inhibiting the replication of the SARS-CoV-2 virus by the compound, and application of the compound in preventing and treating related diseases caused by the SARS-CoV-2 virus, particularly related diseases related to inflammation and autoimmunity.
Background
The novel Coronavirus SARS-CoV-2 and the middle east respiratory syndrome Coronavirus MERS-CoV, the severe acute respiratory syndrome Coronavirus SARS-CoV belong to the family of coronaviridae, and the Coronavirus (Coronavir, CoVs) is an enveloped single-strand positive-strand RNA virus, the genome length of which is about 26000-32000bp, and the Coronavirus is the largest RNA virus known at present.
By 5, 13 and 13 days in 2020, about 420 ten thousand cases and about 29 ten thousand deaths of global new coronary pneumonia are diagnosed. One of the factors that contribute to the mortality rate is the lack of an effective treatment regimen. There is an urgent need for effective antiviral drugs to combat SARS-CoV-2 infection, which not only reduces the disease burden on the patient, but also reduces the risk of the patient infecting others. While developing new drugs takes years to determine their safety and efficacy, it may not be practical to control SARS-CoV-2 infection in a short period of time. The most rapid way is therefore to screen drugs against SARS-CoV-2 infection from FDA marketed drugs. Currently, antiviral drugs have been widely used such as: saquinavir (saquinavir), indinavir (indinavir), ritonavir (ritonavir), nelfinavir (nelfinavir), amprenavir (amprenavir) and lopinavir (lopinavir) are mainly used for Human Immunodeficiency Virus (HIV), a pathogenic retrovirus that causes acquired immunodeficiency syndrome (AIDS) and its associated lesions. The anti-Ebola virus test drug Reddesivir (remdesivir) developed by Gilidische scientific Inc. can inhibit RNA synthetase (RdRp), and hopefully inhibit COVID-19 coronavirus. Therefore, there is an urgent need to find more safe and effective drugs for treating coronavirus. However, the above drugs have toxic side effects and may cause unnecessary damage to the patient himself, so that the search for a less toxic anti-SARS-CoV-2 drug is an urgent solution for treating COVID-19.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a method for treating COVID-19, which avoids the use of drugs with serious toxic and side effects and has stable effect.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a pharmaceutical composition against the novel coronavirus SARS-CoV-2, which comprises an effective amount of one or more of Amlodipine Besylate, Fendiline HCl, DronedaroneHCl, trifluorerazine 2HCl, Tetrandrine, Conivaptan HCl, Nilotinib, Vanderitan (ZD6474), Clofazine, Sertraline HCl, Thiodiazine HCl, Celecoxib, Vortioxetine, Monensin sodium salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin.
Further, the active ingredient in such pharmaceutical compositions may also be a conjugate of the above-described compounds for the purpose of administering a therapeutic molecule or incorporating a label, examples of which include, but are not limited to, alkyl, alkenyl, alkynyl, amido, amino, ether, thioether, ester, alkylene, heteroalkylene, aryl, or heterocyclyl, each of which may be optionally substituted as is well known in the art.
Furthermore, the invention provides the application of the pharmaceutical composition in preparing a medicament for treating or preventing novel coronavirus, wherein the coronavirus is novel coronavirus SARS-CoV-2, MERS-CoV and SARS-CoV.
Further, the present invention provides the use of a pharmaceutical composition comprising one or more of Amlodipine Besylate, Fendiline HCl, Dronedarone HCl, trifluorerazine 2HCl, Tetrandrine, Conivaptan HCl, Nilotinib, Vanderanib (ZD6474), Clofazine, Sertraline HCl, Thioridazine HCl, Celecoxib, Vortioxetine, Monensin sodium salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin for the manufacture of a medicament for the treatment or prevention of a coronavirus;
further, the present invention provides the use of a pharmaceutical composition comprising one or more of Amlodipine Besylate, Fendiline HCl, Dronedarone HCl, trifluorerazine 2HCl, Tetrandrine, Conivaptan HCl, Nilotinib, Vandernib (ZD6474), Clofazine, Sertraline HCl, Thioridazine HCl, Celecoxib, Vortioxetine, Monensin sodium salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin for the manufacture of a medicament for the treatment or prevention of COVID-19 pneumonia following viral infection.
Preferably, the composition is one or more of Nilotinib, Clofazimine, Celecoxib, Actidione, Raloxifene HCl.
Preferably, the composition further comprises at least one therapeutic agent selected from the group consisting of antiviral agents, corticosteroids, immunomodulators, vasoactive drugs and drugs for treating viral infections and/or diseases caused by viral infections and known drugs, preferably wherein the therapeutic agent is an antiviral agent, preferably wherein the therapeutic agent is an immunomodulator;
preferably, the therapeutic agent is selected from the group consisting of interferons, imiquimod, resiquimod, podophyllotoxins, bleomycin, and retinoids;
preferably, wherein said therapeutic agent is selected from interferon- α, lopinavir, linonevir, ribavirin, chloroquine phosphate, abidol;
preferably, the composition further comprises at least one selected from the group consisting of antitussives, mucolytics, expectorants, antipyretics, analgesics and nasal decongestants;
preferably, the virus is a novel coronavirus SARS-CoV-2, MERS-CoV, SARS-CoV.
Further, the present invention provides a use of a pharmaceutical composition for the manufacture of a medicament for treating or preventing covi-19 pneumonia caused by infection with a virus, comprising administering an effective amount of the above pharmaceutical composition and/or pharmaceutically acceptable salts, polymorphs, solvates, hydrates, metabolites, prodrugs or diastereomeric forms thereof; preferably, it comprises a pharmaceutically acceptable carrier.
Further, the above pharmaceutical compositions are administered orally, parenterally, intravenously, intramuscularly, transdermally, via the oral route, subcutaneously or by suppository.
Effects of the invention
The invention has the positive effect that 18 FDA marketed drugs such as Amlodipine Besylate, Fendiline HCl, Dronedarone HCl, trifluorerazine 2HCl, Tetrandrine, Conivaptan HCl, Nilotinib, Vanderanib (ZD6474), Clofazine, Sertraline HCl, Thioridazine HCl, Celecoxib, Vortioxetine, Monensin sodium salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin and the like can effectively inhibit the replication of SARS-CoV-2 virus, and the compounds can directly become drugs for treating SARS-CoV-2.
The results of in vitro cell experiments show that: said 18 medicines and their composition have very small toxic action to Vero normal cell, but can obviously inhibit infection of SARS-CoV-2 virus to normal cell, and at cell level can inhibit SARS-CoV-2, and can obviously reduce virus's toxic value in cell and inhibit its proliferation in cell, and has dose-dependent property.
Therefore, the 18 medicines and the composition thereof can be used as a novel replication inhibitor of the coronavirus SARS-CoV-2, have the potential of treating COVID-19 pneumonia caused by human infection with the virus or other diseases caused by animal infection, and have the advantages of high safety, small toxic and side effects and the like.
Drawings
FIG. 1 shows inhibition of SARS-CoV-2 virus replication in Vero cells by compounds of formula (a) Amlodipine Besylate, (b) Fendiline HCl, (c) Dronedarone HCl, (d) trifluroperazine 2HCl, (e) Tetrandrine, (f) Conivaptan HCl, (g) Nilotinib, (h) Vanderan (ZD6474), (i) Clofazine, (j) Sertraline HCl, (k) Thioridazine HCl, (l) Celecoxib, (m) Vortioxetine, (n) Monensin sodium salt, (o) Actidione, (p) Raloxifene HCl, (q) Temsirolimus (CCI-779, NSC683864), (r) Salifungin
Detailed Description
Vero was purchased from ATCC; SARS-CoV-2 virus is isolated from the sample; one-Step Absolute quantitative PCR enzyme Fast Virus 1-Step Master Mix (4444434), Trizol LS from Thermo; direct-zol of nucleic acid extraction kitTMRNA MiniPrep (R2052) was purchased from Zymo Research; CCK8 kit (40203) was purchased from Shanghai assist in san Biotech Co., Ltd.
1. RNA extraction in supernatant
The operation is carried out according to the instruction of the nucleic acid extraction kit, and the specific steps are as follows:
1) adding equal volume of absolute ethyl alcohol into the sample added with Trizol LS, uniformly mixing, transferring the liquid to Zymo-SpinTMIICR column, which is placed on a collection tube and centrifuged at 10000g for 1min
2) Zymo-SpinTMIICR column transfer to fresh collection tubes
3) In Zymo-SpinTM400 μ l of Direct-zol was added to the IICR columnTMRNA PreWash, 10000g for 1min,after discarding the flow-through liquid, repeating the procedure
4) In Zymo-SpinTMAdding 700 μ l RNA Wash Buffer into IICR column, centrifuging at 10000g for 2min
5) Zymo-SpinTMIICR column was transferred to a clean EP tube, 50. mu.l of nuclease-free water was added, and centrifugation was carried out at 10000g for 1min
6) The RNA sample can be directly used for subsequent experiments or frozen in a refrigerator at-80 DEG C
2. Absolute quantitative PCR
the RNA standard was diluted 10-fold at 1X 109,1×108,……1×104Duplicate wells for each set of samples
Absolute quantitative PCR system
Fast Virus 1-Step Master Mix(4×) | 5μl |
Primer 1 (50. mu.M) | 0.1μl |
Primer 2 (50. mu.M) | 0.1μl |
Probe (20 μ M) | 0.1μl |
Nucleic acids | 5μl |
Water (W) | 9.4μl |
PCR amplification procedure: 50 ℃, 15min, 95 ℃, 3 min; 95 ℃, 15s, 60 ℃, 45s + Plate Read, 50 cycles total
The amplification process, the fluorescence signal detection and the data storage and analysis are all completed by a fluorescence quantitative PCR instrument and self-contained software.
3. Cell viability assay
1) Vero cells were seeded in 96-well plates at 37 ℃ with 5% CO2Culturing overnight in an incubator
2) Different concentrations of drugs (30, 10, 3.33, 1.11, 0.37, 0.12, 0.04, 0.014. mu.M) were added to the plates
3) Incubate the plate in incubator for 24h
4) Add 10. mu.l of CCK8 solution to each well (care not to generate bubbles in the well which would affect the OD reading)
5) Incubating the culture plate in an incubator for 1-4h
6) Absorbance at 450nm was measured with a microplate reader
7) If OD is not to be measured temporarily, 10. mu.l of 0.1M HCl solution or 1% W/V SDS solution may be added to each well, and the plate may be covered and kept at room temperature in the absence of light. The absorbance did not change when measured over 24 hours.
Note that: if the test substance is aerobic or reductive, the drug effect can be removed by replacing the fresh medium (removing the medium and washing the cells twice with medium and then adding new medium) before adding CCK-8. Of course, when the influence of the drug is small, the blank absorption after the drug is added into the culture medium can be directly deducted without replacing the culture medium.
Vitality calculation
Cell viability (%) ([ a (dosed) -a (blank) ]/[ a (0 dosed) -a (blank) ] × 100
A (dosing): absorbance of wells with cells, CCK-8 solution and drug solution
A (blank): absorbance of wells with media and CCK-8 solution without cells
A (0 dosing): absorbance of wells with cells, CCK-8 solution and no drug solution
Cell viability: cell proliferation Activity or cytotoxic Activity
Vero cells were loaded with different concentrations (30, 10, 3.33, 1.11, 0.37, 0.12, 0.04, 0.014. mu.M) of test compound at 37 ℃ with 5% CO2The incubator is treated for 1h, then SARS-CoV-2 virus with 0.1MOI is added, and the incubation is continued for 1h at 37 ℃. Subsequently, the cells were washed once with a blank medium, and the test compound (30, 10, 3.33, 1.11, 0.37, 0.12, 0.04, 0.014. mu.M) was added thereto at different concentrations, and after further culturing until viral infection was completed for 24 hours, RNA from the culture supernatant was extracted, and changes in viral copy number were detected by absolute quantitative PCR, and the viral copy number treated with 0.014. mu.M test compound was taken as the maximum infection value, and the copy numbers at other concentrations were divided by the copy number of the 0.014. mu.M group to obtain the viral replication ratio. Nonlinear fitting (% inhibition) is carried out on the data by utilizing graphpad 7.0 software to obtain the IC of the compound to be detected50。
Vero cells were cultured at 37 ℃ for 24 hours with different concentrations (30, 10, 3.33, 1.11, 0.37, 0.12, 0.04, 0.014. mu.M) of test compound added thereto, and then cell activity was measured using CCK8 kit. Nonlinear fitting (% cell viability) is carried out on the data by utilizing graphpad 7.0 software to obtain CC of the compound to be detected50。
By SI ═ CC50/IC50(CC 50: cytoxicity concentration, drug concentration at which 50% of the cells were killed IC 50: inhibition concentration, half maximal inhibitory concentration), and the results obtained by calculation are shown in Table 1.
TABLE 1 summary of drugs inhibiting SARS-CoV-2
As can be seen from the data: for those skilled in the art, the higher the SI value in the process of screening drugs, the lower the drug toxicity, the better the SI is generally greater than 10 in the drug screening experiment, and the SI of Nilotinib, Clofazimine, Celecoxib, Actidione, and Raloxifene HCl in the above drugs is far greater than 10, which indicates that the above drugs have significant drug application prospects, and further research on them can be performed.
Also, as can be seen from the results in fig. 1, as the concentration of the added compound increases, the viral titer decreases, being dose dependent, and the preparation of the drug provides a consideration. If the medicine is applied to clinic, one of the reasons is that the toxicity of the medicine is low, and the medicine does not bring great side effects to human bodies. Wherein a higher CC50 indicates a higher concentration of drug that causes 50% cell death; the lower the IC50, the lower the concentration of drug indicating that the inhibition efficiency reaches 50%; therefore, the higher the CC50, the lower the IC50, the higher the SI value, and the lower the drug toxicity.
From the analysis in table 1, it is known that drugs effective for coronavirus infection belong to a wide range of drug therapies including those for treating neurological diseases, hormones, ion channels, enzymes, and some antibacterial agents, etc.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, the invention is intended to encompass any variations, uses, or adaptations of the invention following, in general, the principles of the invention, including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains.
Claims (10)
1. An anti-coronavirus pharmaceutical composition, said medicament capable of inhibiting the replication of SARS-CoV-2 virus, said medicament comprising an effective amount of one or more of Amlodipine Besylate, Fendiline HCl, Dronedarone HCl, trifluorerazine 2HCl, Tetrandrine, Conivaptan HCl, Nilotinib, Vandernib (ZD6474), Clofazine, Sertraline HCl, Thioridazine HCl, Celecoxib, Vortioxetine, Monensinoid salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin.
2. The pharmaceutical composition of claim 1, wherein the active ingredient is a conjugate of the above compounds for the administration of a therapeutic molecule or the incorporation of a label, examples of which include, but are not limited to, alkyl, alkenyl, alkynyl, amido, amino, ether, thioether, ester, alkylene, heteroalkylene, aryl, or heterocyclyl, each of which may be optionally substituted.
3. The pharmaceutical composition of claim 1, wherein the coronavirus is a novel coronavirus SARS-CoV-2, MERS-CoV, SARS-CoV, preferably the coronavirus is a novel coronavirus SARS-CoV-2.
4. Use of a pharmaceutical composition comprising one or more of Amlodipine Besylate, Fendiline HCl, Dronedarone HCl, trifluorerazine 2HCl, Tetrandrine, Conivaptan HCl, Nilotinib, Vandanib (ZD6474), Clofazine, Sertraline HCl, Thioridazine HCl, Celecoxib, Vortioxetine, Monensin sodium salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin in the manufacture of a medicament for the treatment or prevention of a coronavirus.
5. Use of a pharmaceutical composition comprising one or more of Amlodipine Besylate, Fendiline HCl, Dronedarone HCl, trifluorerazine 2HCl, Tetrandrine, comivaptan HCl, Nilotinib, Vandetanib (ZD6474), Clofazimine, Sertraline HCl, Thioridazine HCl, Celecoxib, Vortioxetine, ensodinium salt, Actidione, Raloxifene HCl, Temsirolimus (CCI-779, NSC683864), Salifungin, preferably one or more of Nilotinib, Clofazimine, Celecoxib, diediketine HCl, Raloxifene HCl, for the manufacture of a medicament for the treatment or prevention of coviv-19 pneumonia following infection with the virus.
6. The use according to claim 4 or 5, comprising at least one therapeutic agent selected from antiviral agents, corticosteroids, immunomodulators, vasoactive drugs and drugs for the treatment of viral infections and/or diseases caused by viral infections and known drugs, preferably wherein said therapeutic agent is an antiviral agent; wherein said therapeutic agent is an immunomodulatory agent, further preferred wherein said therapeutic agent is selected from the group consisting of interferons, imiquimod, resiquimod, podophyllotoxin, bleomycin and retinoids; further preferably, the therapeutic agent is selected from interferon- α, lopinavir, linonevir, ribavirin, chloroquine phosphate, and abidol; further preferably, the pharmaceutical composition further comprises at least one selected from the group consisting of antitussives, mucolytics, expectorants, antipyretics, analgesics and nasal decongestants.
7. Use according to claim 4 or 5, wherein the coronavirus is a novel coronavirus SARS-CoV-2, MERS-CoV, SARS-CoV, preferably wherein the coronavirus is a novel coronavirus SARS-CoV-2.
8. The use of claim 4 or 5, comprising administering an effective amount of the pharmaceutical composition of claim 1 and/or a pharmaceutically acceptable salt, polymorph, solvate, hydrate, metabolite, prodrug or diastereomeric form thereof.
9. Use according to claim 4 or 5, characterized in that the pharmaceutical composition according to claim 1 is contained in a pharmaceutically acceptable carrier.
10. The use of claim 4 or 5, wherein the pharmaceutical composition of claim 1 is administered orally, parenterally, intravenously, intramuscularly, transdermally, via the oral route, subcutaneously or by suppository.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111416996.0A CN114191553B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
CN202010461381.9A CN111728973A (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
CN202111416989.0A CN114191552B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010461381.9A CN111728973A (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111416989.0A Division CN114191552B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
CN202111416996.0A Division CN114191553B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
Publications (1)
Publication Number | Publication Date |
---|---|
CN111728973A true CN111728973A (en) | 2020-10-02 |
Family
ID=72647808
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111416996.0A Active CN114191553B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
CN202111416989.0A Active CN114191552B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
CN202010461381.9A Pending CN111728973A (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111416996.0A Active CN114191553B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
CN202111416989.0A Active CN114191552B (en) | 2020-05-27 | 2020-05-27 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
Country Status (1)
Country | Link |
---|---|
CN (3) | CN114191553B (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112138006A (en) * | 2020-10-08 | 2020-12-29 | 华中农业大学 | Application of R848 in preparation of medicine for inhibiting novel coronavirus SARS-CoV-2 |
CN112137993A (en) * | 2020-09-16 | 2020-12-29 | 南方医科大学 | Application of sertraline hydrochloride and derivatives thereof in preparation of novel coronavirus resistant medicines |
CN112315960A (en) * | 2020-10-23 | 2021-02-05 | 武汉珈创生物技术股份有限公司 | Application of benidipine in preparation of medicine for resisting novel coronavirus infectious diseases |
WO2021043234A1 (en) * | 2019-09-04 | 2021-03-11 | City University Of Hong Kong | Use of berbamine or its analogue for preventing or treating rna virus infection |
CN113735929A (en) * | 2021-07-21 | 2021-12-03 | 海化生命(厦门)科技有限公司 | Anti-coronavirus compound and preparation method and application thereof |
WO2022094480A1 (en) * | 2020-11-02 | 2022-05-05 | Skymount Medical Us Inc. | Methods of treating coronavirus disease and compounds for same |
EP4000606A1 (en) * | 2020-11-18 | 2022-05-25 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Compounds for coronavirus infection treatment and/or prevention |
US11357771B2 (en) | 2019-09-04 | 2022-06-14 | City University Of Hong Kong | Methods of preventing or treating flavivirus virus infections and methods of inhibiting the entry of flvivirus, enterovirus or lentivirus into host cells |
CN114632155A (en) * | 2022-03-24 | 2022-06-17 | 中国人民解放军军事科学院军事医学研究院 | PKA inhibitor H89 and application of siRNA thereof in preparation of drugs for treating novel coronavirus |
EP4129287A1 (en) * | 2021-08-04 | 2023-02-08 | Dompe' Farmaceutici S.P.A. | Raloxifene for use in the treatment of sars-cov-2 variants infections |
WO2023036820A1 (en) * | 2021-09-10 | 2023-03-16 | H. Lundbeck A/S | Therapeutic uses of 1-[2-(2,4-dimethyl-phenylsulfanyl)phenyl]piperazine |
CN116115615A (en) * | 2021-12-07 | 2023-05-16 | 北京中医药大学 | Broad-spectrum antiviral medicine, its medicinal composition and application |
US12102634B2 (en) | 2020-09-04 | 2024-10-01 | 6J Biotechnology (Hong Kong) Limited | Use of berbamine or its analogue for preventing or treating RNA virus infection |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114191553B (en) * | 2020-05-27 | 2024-01-02 | 中国医学科学院病原生物学研究所 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101658523A (en) * | 2008-04-01 | 2010-03-03 | 立业制药股份有限公司 | New application of clofazimine for curing tuberculosis |
CN111150723A (en) * | 2020-02-06 | 2020-05-15 | 北京佳诚医药有限公司 | Application of tranexamic acid injection as pneumonia treatment medicine |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104119298B (en) * | 2014-08-13 | 2016-08-24 | 北京蓝贝望生物医药科技股份有限公司 | Hydrobromic acid Wo Saiting or hydrobromic acid are fertile for Xi Ting |
US9399039B1 (en) * | 2015-06-30 | 2016-07-26 | University Of South Florida | Inhibitors of the FKBP51 protein from a high-throughput drug screen and methods of use |
US10071062B2 (en) * | 2015-12-08 | 2018-09-11 | Cipla Limited | Methods for the treatment of hepatitis C |
EP3494141A4 (en) * | 2016-08-03 | 2020-04-08 | Bio-Techne Corporation | Identification of vsig3/vista as a novel immune checkpoint and use thereof for immunotherapy |
CN111135184A (en) * | 2020-03-05 | 2020-05-12 | 华中农业大学 | Application of GS-441524 in preparation of novel coronavirus SARS-CoV-2 inhibitor |
CN114191553B (en) * | 2020-05-27 | 2024-01-02 | 中国医学科学院病原生物学研究所 | Medicine for resisting novel coronavirus SARS-CoV-2 and its application |
-
2020
- 2020-05-27 CN CN202111416996.0A patent/CN114191553B/en active Active
- 2020-05-27 CN CN202111416989.0A patent/CN114191552B/en active Active
- 2020-05-27 CN CN202010461381.9A patent/CN111728973A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101658523A (en) * | 2008-04-01 | 2010-03-03 | 立业制药股份有限公司 | New application of clofazimine for curing tuberculosis |
CN111150723A (en) * | 2020-02-06 | 2020-05-15 | 北京佳诚医药有限公司 | Application of tranexamic acid injection as pneumonia treatment medicine |
Non-Patent Citations (1)
Title |
---|
YI-YU KE等: "Artificial intelligence approach fighting COVID-19 with repurposing drugs", 《BIOMEDICAL JOURNAL》 * |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021043234A1 (en) * | 2019-09-04 | 2021-03-11 | City University Of Hong Kong | Use of berbamine or its analogue for preventing or treating rna virus infection |
US11357771B2 (en) | 2019-09-04 | 2022-06-14 | City University Of Hong Kong | Methods of preventing or treating flavivirus virus infections and methods of inhibiting the entry of flvivirus, enterovirus or lentivirus into host cells |
US12102634B2 (en) | 2020-09-04 | 2024-10-01 | 6J Biotechnology (Hong Kong) Limited | Use of berbamine or its analogue for preventing or treating RNA virus infection |
CN112137993A (en) * | 2020-09-16 | 2020-12-29 | 南方医科大学 | Application of sertraline hydrochloride and derivatives thereof in preparation of novel coronavirus resistant medicines |
CN112138006A (en) * | 2020-10-08 | 2020-12-29 | 华中农业大学 | Application of R848 in preparation of medicine for inhibiting novel coronavirus SARS-CoV-2 |
CN112315960A (en) * | 2020-10-23 | 2021-02-05 | 武汉珈创生物技术股份有限公司 | Application of benidipine in preparation of medicine for resisting novel coronavirus infectious diseases |
CN112315960B (en) * | 2020-10-23 | 2021-08-13 | 武汉珈创生物技术股份有限公司 | Application of benidipine in preparation of medicine for resisting novel coronavirus infectious diseases |
WO2022094480A1 (en) * | 2020-11-02 | 2022-05-05 | Skymount Medical Us Inc. | Methods of treating coronavirus disease and compounds for same |
WO2022106489A1 (en) * | 2020-11-18 | 2022-05-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Compounds for coronavirus infection treatment and/or prevention |
EP4000606A1 (en) * | 2020-11-18 | 2022-05-25 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Compounds for coronavirus infection treatment and/or prevention |
CN113735929A (en) * | 2021-07-21 | 2021-12-03 | 海化生命(厦门)科技有限公司 | Anti-coronavirus compound and preparation method and application thereof |
EP4129287A1 (en) * | 2021-08-04 | 2023-02-08 | Dompe' Farmaceutici S.P.A. | Raloxifene for use in the treatment of sars-cov-2 variants infections |
WO2023012233A1 (en) * | 2021-08-04 | 2023-02-09 | Dompe' Farmaceutici Spa | Raloxifene for use in the treatment of sars-cov-2 variants infections |
WO2023036820A1 (en) * | 2021-09-10 | 2023-03-16 | H. Lundbeck A/S | Therapeutic uses of 1-[2-(2,4-dimethyl-phenylsulfanyl)phenyl]piperazine |
CN116115615A (en) * | 2021-12-07 | 2023-05-16 | 北京中医药大学 | Broad-spectrum antiviral medicine, its medicinal composition and application |
CN114632155A (en) * | 2022-03-24 | 2022-06-17 | 中国人民解放军军事科学院军事医学研究院 | PKA inhibitor H89 and application of siRNA thereof in preparation of drugs for treating novel coronavirus |
Also Published As
Publication number | Publication date |
---|---|
CN114191552A (en) | 2022-03-18 |
CN114191553B (en) | 2024-01-02 |
CN114191553A (en) | 2022-03-18 |
CN114191552B (en) | 2024-01-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN114191552B (en) | Medicine for resisting novel coronavirus SARS-CoV-2 and its application | |
US20210346411A1 (en) | Use of substituted aminopropionate compounds in treatment of sars-cov-2 infection | |
US11318135B2 (en) | Use of Favipiravir in treatment of coronavirus infection | |
US10894035B2 (en) | Use of indole compounds to stimulate the immune system | |
CN111743899B (en) | Application of nitazoxanide and its active form tizoxanide in preparing medicine for treating SARS-CoV-2 infection | |
CN114028453B (en) | Broad-spectrum antiviral medicine, its medicinal composition and application | |
CN109364074B (en) | Application of 6-aminonicotinamide as effective component in preparing medicament for treating hepatitis B | |
EP0424193B1 (en) | Use of actinonin for the manufacture of a medicament for angiogenesis inhibition | |
JPH05507719A (en) | pharmaceutical treatment | |
CN115804775A (en) | Application of S63845 in preparation of anti-neocoronavirus infection medicine | |
CN111686111B (en) | Application of MALT1 protease inhibitor in preparation of non-small cell lung cancer therapeutic drug | |
RU2401121C1 (en) | Pharmaceutical composition based on herbal extracts for treatment and prevention of viral infections and syphilis and method of treatment and prevention of viral infections and syphilis | |
CN111568900A (en) | Application of indomethacin in resisting coronavirus infection | |
CN117899065B (en) | Antiviral drug and application of dehydrodiisoeugenol in preparation of antiviral drug | |
CN113712976B (en) | Application of small molecular compound phytic acid sodium hydrate in preparing anti-SARS-CoV-2 medicine | |
EP3344244A1 (en) | Use of indole compounds to stimulate the immune system | |
US20230071014A1 (en) | Application of ritonavir in treating sars-cov-2 infection | |
CN107540631A (en) | Application of the amino carboxylic acid esters compound in terms of zika virus infection is treated | |
JPH1179992A (en) | Apoptosis suppressant | |
CN118178405A (en) | Application of mebendazole in preparing medicament for preventing or treating hepatic fibrosis | |
CN114515291A (en) | Application of small molecular compound piperacillin in preparing anti-SARS-CoV-2 medicine | |
TW383304B (en) | 1-(3-aminoindazol-5-yl)-3-butyl-cyclic urea useful as a HIV protease inhibitor, and pharmaceutical compositions and kits thereof | |
CN117899065A (en) | Antiviral drug and application of dehydrodiisoeugenol in preparation of antiviral drug | |
Ghauri et al. | Debate on the Effectiveness of Hydroxychloroquine for Treatment of Coronavirus Disease 2019 (COVID-19) | |
CN117510409A (en) | Broad-spectrum antiviral traditional Chinese medicine monomer bat Ge Sulin alkali, and pharmaceutical composition and application thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |