CN111715649A

CN111715649A - Reagent bottle cleaning and sterilizing equipment for genetic engineering

Info

Publication number: CN111715649A
Application number: CN202010615326.0A
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-30
Filing date: 2020-06-30
Publication date: 2020-09-29

Abstract

The invention relates to the technical field of genetic engineering, in particular to reagent bottle cleaning and disinfecting equipment for genetic engineering. The invention utilizes high-temperature steam to disinfect the reagent bottle and also utilizes the steam to drive the cleaning mechanism in the cylinder body to clean the bottle body.

Description

Reagent bottle cleaning and sterilizing equipment for genetic engineering

Technical Field

The invention relates to the technical field of genetic engineering, in particular to a reagent bottle cleaning and disinfecting device for genetic engineering.

Background

The gene engineering is also called gene splicing technology and DNA recombination technology, and is characterized by that it uses molecular genetics as theoretical basis, and uses the modern methods of molecular biology and microbiology as means, and makes the genes with different sources according to the predesigned blueprint to construct hybrid DNA molecule in vitro, then introduces it into living cell so as to change the original genetic characteristics of organism, obtain new variety and produce new product. The gene engineering technology provides a powerful means for the research of the structure and the function of the gene.

In the experimental process of genetic engineering, a reagent bottle is required to be used for loading various different reagents; because the genetic engineering has high requirements on the experimental precision, the reagent bottle needs to be thoroughly and completely cleaned and disinfected before the experiment, so that the influence of impurities in the reagent bottle on the reagent and the interference on the experimental result are prevented; most of the existing reagent bottle cleaning and disinfecting equipment is used for cleaning and disinfecting reagent bottles, and the disinfection mode is usually that boiling water, a steam sterilizer or a chemical sterilizing agent is used for disinfecting the bottles; in the process of cleaning and disinfecting the reagent bottle, complete and thorough disinfection and cleaning can be easily achieved for cleaning and disinfecting the outer surface of the reagent bottle body, but when the inner wall of the reagent bottle body is cleaned and disinfected, the inner wall of the reagent bottle body is very inconvenient to clean due to the narrow mouth of the reagent bottle, and complete cleaning is difficult to achieve; the reagent bottle is disinfected by using boiling water, a large amount of hot water is needed, water resources are wasted, and the reagent bottle is inconvenient to take out of the boiling water; the bottle is disinfected by using the chemical sterilizing agent, although the bottle can be completely disinfected, chemical substances are remained on the reagent bottle after the bottle is disinfected, and the reagent is still interfered because the reagent bottle is not cleaned completely. The cleaning and disinfecting equipment combining two steps of cleaning and disinfecting is designed, so that the reagent bottle can be conveniently, quickly and completely cleaned and disinfected.

Disclosure of Invention

The invention aims to solve the problems of low efficiency and difficulty in fully and completely cleaning and disinfecting the inner wall of a reagent bottle caused by separation of a disinfecting process and a cleaning process, and provides reagent bottle cleaning and disinfecting equipment for genetic engineering.

In order to achieve the purpose, the invention adopts the following technical scheme:

the reagent bottle cleaning and disinfecting equipment for genetic engineering comprises a barrel body, wherein a conical hopper communicated with the inside of the barrel body is fixedly arranged at the bottom end of the barrel body, a pipe body communicated with the inside of the conical hopper is fixedly arranged at the bottom end of the conical hopper, the barrel body, the conical hopper and the pipe body are coaxially arranged, a transverse plate is fixedly arranged in the barrel body, a rotating shaft is rotatably arranged on the transverse plate, an impeller is fixedly arranged at the bottom end of the rotating shaft, the top end of the rotating shaft extends upwards until the rotating shaft extends into the barrel body, a cleaning module is fixedly arranged in the barrel body and is in transmission connection with the cleaning module, two side plates which are symmetrically distributed are fixedly arranged on the outer surface of the lower part of the barrel body along the circumferential direction, a supporting leg is fixedly arranged on the lower surface of each fixing, the top cover is fixedly provided with a bottle body fixing mechanism corresponding to the cleaning module, and the top cover is also fixedly provided with a cover body opening and closing module.

Preferably, the cleaning module comprises a fixing plate, a first hollow pipe, a second hollow pipe, bristles and a transmission mechanism, wherein a plurality of fixing plates which are uniformly distributed are fixedly arranged on the inner wall of the barrel along the circumferential direction and are arranged on the fixing plate in a penetrating manner, the first hollow pipe is fixedly connected to the fixing plate and is arranged in parallel to the central axis of the barrel, and each fixing plate is arranged on the top end of the first hollow pipe in a rotating manner and is provided with the second hollow pipe which is coaxially arranged with the first hollow pipe, the first hollow pipe is communicated with the second hollow pipe, the outer wall of the second hollow pipe is fixedly provided with the bristles and each bristle along the circumferential direction and is connected with the rotating shaft through the transmission mechanism.

Preferably, the transmission mechanism comprises a main gear and a pinion, each second hollow tube is fixedly provided with a pinion which is coaxially arranged with the second hollow tube, the top end of the rotating shaft is fixedly provided with a main gear which is coaxially arranged with the rotating shaft, and the main gear is meshed with each pinion.

Preferably, bottle fixed establishment includes third hollow tube, a location section of thick bamboo, air duct, sucking disc, the top cap lower surface has a plurality of evenly distributed's third hollow tube, every along circumference fixed mounting the equal fixed mounting in third hollow tube bottom has a location section of thick bamboo with it coaxial setting, the centre fixed mounting of department in the top in the location section of thick bamboo has the sucking disc, it is equipped with the air duct to insert in the third hollow tube, the air duct bottom is connected with the sucking disc, the air duct top upwards extends until running through the top cap.

Preferably, the cover body opening and closing module comprises a supporting bent rod, a top plate, a threaded rod, a spring and a top column, the upper surfaces of the two side plates are fixedly provided with the supporting bent rod, the two supporting bent rods are correspondingly arranged, a top plate is fixedly arranged between the inner side tail ends of the upper parts of the two supporting bent rods, two threaded rods are arranged on the top plate in a penetrating way, the bottom ends of the two threaded rods are fixedly arranged on the top cover, the threaded rods above the top plate are respectively provided with a limiting nut, the threaded rods between the top plate and the top cover are respectively sleeved with a spring, two ends of the spring are respectively and fixedly connected with the corresponding top plate and the top cover, the top plate is also provided with a top post in a penetrating way, the top pillar bottom contacts with the top cap upper surface, the top pillar top upwards extends to the roof top, top pillar top fixed mounting has stop gear.

Preferably, the bottom end of the top column is fixedly provided with a chassis.

Preferably, stop gear includes top dish, limit threaded rod, it is provided with limit threaded rod to run through on the top dish to push up post top fixed mounting, limit threaded rod bottom fixed connection is on the roof, install the nut on the limit threaded rod of top dish top.

The invention provides a reagent bottle cleaning and disinfecting equipment for genetic engineering, which has the beneficial effects that: on one hand, the reagent bottle is sterilized at high temperature by using high-temperature steam without chemical substance residues, and on the other hand, the steam drives the impeller to drive the rotating shaft to rotate, so that the cleaning module is driven to clean the inside of the reagent bottle, and the sterilization and cleaning processes of the reagent bottle are combined together, so that the reagent bottle is more efficient; simultaneously when the brush hair on the second hollow tube carries out the cleanness to the reagent bottle inner wall, the steam of high temperature passes through first hollow tube, second hollow tube and gets into the reagent bottle in, accomplishes the thorough abundant disinfection to the reagent bottle inner wall.

Drawings

FIG. 1 is a first structural schematic diagram of a reagent bottle cleaning and disinfecting device for genetic engineering according to the present invention;

FIG. 2 is a schematic structural diagram II of a reagent bottle cleaning and disinfecting apparatus for genetic engineering according to the present invention;

FIG. 3 is a schematic sectional view of a reagent bottle cleaning and disinfecting apparatus for genetic engineering according to the present invention;

FIG. 4 is a schematic sectional view of a reagent bottle cleaning and disinfecting apparatus for genetic engineering according to the present invention;

FIG. 5 is a schematic view of a portion of the structure of FIG. 3 at A;

fig. 6 is a partial structural view at B of fig. 4.

In the figure: the device comprises a barrel body 1, a conical hopper 2, a tube body 3, a transverse plate 4, a rotating shaft 5, an impeller 6, a main gear 7, a fixing plate 8, a first hollow tube 9, a second hollow tube 10, bristles 11, a pinion 12, a top cover 13, a third hollow tube 14, a positioning barrel 15, an air duct 16, a suction cup 17, a side plate 18, a supporting leg 19, a bottom plate 20, a supporting bent rod 21, a top plate 22, a threaded rod 23, a spring 24, a top column 25, a bottom plate 26, a top plate 27 and a limiting threaded rod 28.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Example 1

Referring to fig. 1-6, a reagent bottle cleaning and sterilizing device for genetic engineering, comprising a barrel body 1, a conical hopper 2 fixedly arranged at the bottom end of the barrel body 1 and communicated with the interior, a tube body 3 fixedly arranged at the bottom end of the conical hopper 2 and communicated with the interior, the barrel body 1, the conical hopper 2 and the tube body 3 being coaxially arranged, a transverse plate 4 fixedly arranged in the tube body 3, a rotating shaft 5 rotatably arranged on the transverse plate 4, an impeller 6 fixedly arranged at the bottom end of the rotating shaft 5, a cleaning module fixedly arranged in the barrel body 1 and extending upwards from the top end of the rotating shaft 5, the rotating shaft 5 being in transmission connection with the cleaning module, two side plates 18 symmetrically distributed along the circumferential direction and fixedly arranged on the outer surface of the lower part of the barrel body 1, a supporting leg 19 fixedly arranged on the lower surface of each fixing plate 18, a bottom plate supporting leg 20, the top cover 13 is fixedly provided with a bottle body fixing mechanism corresponding to the cleaning module, and the top cover 13 is also fixedly provided with a cover body opening and closing module. When the device is used, the device is matched with a steam supply device for use, on one hand, high-temperature steam is used for carrying out high-temperature disinfection on the reagent bottle, on the other hand, the steam driving impeller 6 is used for driving the rotating shaft 5 to rotate, and therefore the cleaning module is driven to clean the reagent bottle.

Cleaning module includes fixed plate 8, first hollow tube 9, second hollow tube 10, brush hair 11, drive mechanism, 1 inner wall of barrel has evenly distributed's a plurality of fixed plates 8 along circumference fixed mounting, it is equipped with a first hollow tube 9 all to run through on every

fixed plate

8, 9 fixed connection of first hollow tube is on fixed plate 8, and every first hollow tube 9 all with 1 axis parallel arrangement of barrel, 9 tops of every first hollow tube all can rotate the second hollow tube 10 of installing coaxial setting with it, and first hollow tube 9 is linked together with second

hollow tube

10, 10 outer walls of second hollow tube are along the circumference fixed brush hair 11 that is equipped with, every second hollow tube 10 all is connected with 5 transmissions of pivot through drive mechanism. The arrangement of the two hollow pipes can facilitate the high-temperature steam in the barrel body 1 to enter the reagent bottle, so that the reagent bottle can be thoroughly cleaned and disinfected without dead angles.

The transmission mechanism comprises a main gear 7 and auxiliary gears 12, each second hollow pipe 10 is fixedly provided with the auxiliary gear 12 which is coaxially arranged with the second hollow pipe, the top end of the rotating shaft 5 is fixedly provided with the main gear 7 which is coaxially arranged with the rotating shaft, and the main gear 7 is meshed with each auxiliary gear 12. The driving of the plurality of cleaning mechanisms in the wash module is accomplished simultaneously by simultaneously driving the plurality of pinions 12 to rotate with the single main gear 7.

The bottle body fixing mechanism comprises a third hollow tube 14, a positioning cylinder 15, an air duct 16 and a sucker 17, wherein the lower surface of the top cover 13 is fixedly provided with a plurality of uniformly distributed third hollow tubes 14 along the circumferential direction, the bottom end of each third hollow tube 14 is fixedly provided with the positioning cylinder 15 coaxially arranged with the bottom end of the third hollow tube, the sucker 17 is fixedly arranged at the center of the top in the positioning cylinder 15, the air duct 16 is inserted into the third hollow tube 14, the bottom end of the air duct 16 is connected with the sucker 17, and the top end of the air duct 16 upwards extends until the top cover 13. The positioning cylinder 15 is used for positioning the reagent bottle to ensure that the reagent bottle can be aligned with a single cleaning mechanism in the cleaning module, and meanwhile, the suction disc 17 is convenient to adsorb the bottle body.

When the reagent bottle fixing device is used, a user connects the top end of each air duct 16 with the air suction mechanism through the hose, then opens the top cover 13, then starts the air suction mechanism, then inserts the bottom of the reagent bottle with the outer surface cleaned into the positioning cylinder 15, and at the moment, the sucker 17 sucks the bottle body of the reagent bottle, so that the reagent bottle is fixed in the positioning cylinder 15; then the user closes the top cover 13, and the second hollow tube 10 in the cleaning module with the bristles 11 arranged thereon is inserted into the corresponding reagent bottle; finally, a user connects the bottom end of the tube body 3 with a steam supply mechanism through a steam delivery pipe, when high-temperature steam generated by the steam supply mechanism passes through the tube body 3, the impeller 6 at the bottom end of the rotating shaft 5 is pushed to rotate, so that the rotating shaft 5 is driven to rotate, the rotating shaft 5 drives the main gear 7 to rotate, the rotating gear 7 simultaneously drives the plurality of auxiliary gears 12 to rotate, the rotating auxiliary gears 12 drive the corresponding second hollow tubes 10 to rotate, so that bristles on the second hollow tubes 10 are utilized to clean the inner walls of the reagent bottles, and when the reagent bottles are cleaned, the high-temperature steam enters the reagent bottles through the first hollow tubes 9 and the second hollow tubes 10 to finish high-temperature disinfection on the inner walls of the reagent bottles; after the cleaning and disinfection are finished, the top cover 13 is opened, and the reagent bottle is taken down.

Example 2

Referring to fig. 1-4, as another preferred embodiment of the present invention, the difference from embodiment 1 is that the cover opening and closing module includes a supporting curved bar 21, a top plate 22, a threaded rod 23, a spring 24, and a top pillar 25, the upper surfaces of two side plates 18 are each fixedly installed with one supporting curved bar 21, and the two supporting curved bars 21 are arranged correspondingly, a top plate 22 is fixedly installed between the inner ends of the upper portions of the two supporting curved bars 21, the top plate 22 is provided with two threaded rods 23 in a penetrating manner, the bottom ends of the two threaded rods 23 are each fixedly installed on the top cover 13, the threaded rod 23 above the top plate 22 is provided with a limit nut, the threaded rod 23 between the top plate 22 and the top cover 13 is provided with one spring 24 in a sleeving manner, the two ends of the spring 24 are respectively fixedly connected to the corresponding top plate 22 and the top cover 13, the top plate 22 is further provided with one top pillar 25, the top of the top pillar 25 extends upwards to the upper part of the top plate 22, and the top end of the top pillar 25 is fixedly provided with a limiting mechanism. The lid subassembly that opens and shuts that sets up does not need the user to take off the lid with the hand, avoids the steam in the barrel 1 to user's injury, uses more convenience, safety.

The bottom end of the top column 25 is fixedly provided with a bottom plate 26. The bottom plate 26 is made of rubber, so that the top cover 13 can be effectively prevented from being damaged by the top column 25. Stop gear includes top dish 27, limit threaded rod 28, and it is provided with limit threaded rod 28 to run through on the top dish 27 to push up post 25 top fixed mounting, and limit threaded rod 28 bottom fixed connection installs the nut on the limit threaded rod 28 of top dish 27 top on the roof 22. The limiting mechanism is used for limiting the upward movement of the top plate 27.

When the invention is used, when the top cover 13 needs to be opened, a user only needs to dismount the nut on the limit threaded rod 28, the spring 24 contracts, so that the top cover 13 is pulled upwards, and the top cover 13 is lifted along the two threaded rods 23 under the pulling action of the two springs 24; when the top cover 13 needs to be closed, a user only needs to press the top disc 27 downwards, so that the top cover 13 is pressed downwards by the aid of the top column 25, finally, the nut on the limiting threaded rod 28 is installed back, the top disc 27 is limited to move upwards by the aid of the nut, and accordingly the top column 25 is limited to move upwards.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. A reagent bottle cleaning and disinfecting device for genetic engineering comprises a barrel body (1) and is characterized in that a conical hopper (2) communicated with the inside of the barrel body (1) is fixedly installed at the bottom end of the barrel body (1), a pipe body (3) communicated with the inside of the conical hopper (2) is fixedly installed at the bottom end of the conical hopper (2), the barrel body (1), the conical hopper (2) and the pipe body (3) are coaxially arranged, a transverse plate (4) is fixedly arranged in the pipe body (3), a rotating shaft (5) is rotatably installed on the transverse plate (4), an impeller (6) is fixedly installed at the bottom end of the rotating shaft (5), the top end of the rotating shaft (5) extends upwards until extending into the barrel body (1), a cleaning module is fixedly installed in the barrel body (1), the rotating shaft (5) is in transmission connection with the cleaning module, and two side plates (18) which are symmetrically distributed are, every the equal fixed mounting of fixed plate (18) lower surface has a supporting leg (19), every the equal fixed mounting in supporting leg (19) bottom has a bottom plate (20), top cap (13) are installed to barrel (1) top end opening part, fixed mounting has and washs the corresponding bottle fixed establishment of module on top cap (13), it has the lid module that opens and shuts to go back fixed mounting on top cap (13).

2. The reagent bottle cleaning and disinfecting device for genetic engineering according to claim 1, wherein the cleaning module comprises a fixing plate (8), a first hollow tube (9), a second hollow tube (10), bristles (11) and a transmission mechanism, wherein a plurality of fixing plates (8) are circumferentially and fixedly arranged on the inner wall of the cylinder body (1) and are uniformly distributed, a first hollow tube (9) penetrates through each fixing plate (8), the first hollow tube (9) is fixedly connected to the fixing plate (8), each first hollow tube (9) is parallel to the central axis of the cylinder body (1), a second hollow tube (10) is rotatably arranged at the top end of each first hollow tube (9) and is coaxially arranged with the first hollow tube (9), the first hollow tube (9) is communicated with the second hollow tube (10), and the bristles (11) are circumferentially and fixedly arranged on the outer wall of the second hollow tube (10), each second hollow pipe (10) is in transmission connection with the rotating shaft (5) through a transmission mechanism.

3. The reagent bottle cleaning and disinfecting device for genetic engineering according to claim 1, characterized in that the transmission mechanism comprises a main gear (7) and a pinion (12), the pinion (12) is coaxially arranged on each second hollow tube (10), the main gear (7) is coaxially arranged on the top end of the rotating shaft (5), and the main gear (7) is meshed with each pinion (12).

4. The reagent bottle cleaning and disinfecting device for genetic engineering according to claim 1, wherein the bottle body fixing mechanism comprises a third hollow tube (14), a positioning cylinder (15), an air duct (16) and a sucker (17), the third hollow tube (14) is circumferentially and fixedly installed on the lower surface of the top cover (13), the positioning cylinder (15) is coaxially installed at the bottom end of each third hollow tube (14), the sucker (17) is fixedly installed at the center of the top in the positioning cylinder (15), the air duct (16) is inserted into each third hollow tube (14), the bottom end of the air duct (16) is connected with the sucker (17), and the top end of the air duct (16) extends upwards until penetrating through the top cover (13).

5. The reagent bottle cleaning and disinfecting device for genetic engineering as claimed in claim 1, wherein the cover body opening and closing module comprises supporting bent rods (21), a top plate (22), threaded rods (23), springs (24) and a top column (25), wherein one supporting bent rod (21) is fixedly mounted on the upper surface of each of the two side plates (18), the two supporting bent rods (21) are correspondingly arranged, a top plate (22) is fixedly mounted between the inner ends of the upper parts of the two supporting bent rods (21), the top plate (22) is provided with two threaded rods (23) in a penetrating manner, the bottom ends of the two threaded rods (23) are fixedly mounted on the top cover (13), a limiting nut is mounted on each threaded rod (23) above the top plate (22), each threaded rod (23) between the top plate (22) and the top cover (13) is sleeved with one spring (24), the both ends of spring (24) are fixed connection respectively on roof (22) and top cap (13) that correspond, it is provided with a fore-set (25) still to run through on roof (22), fore-set (25) bottom contacts with top cap (13) upper surface, fore-set (25) top upwards extends to roof (22) top, fore-set (25) top fixed mounting has stop gear.

6. The cleaning and disinfecting device for the reagent bottle for genetic engineering according to claim 1 is characterized in that a bottom plate (26) is fixedly installed at the bottom end of the top column (25).

7. The reagent bottle cleaning and disinfecting device for genetic engineering as claimed in claim 1, wherein the limiting mechanism comprises a top plate (27) and a limiting threaded rod (28), the top end of the top column (25) is fixedly provided with the limiting threaded rod (28) which penetrates through the top plate (27), the bottom end of the limiting threaded rod (28) is fixedly connected to the top plate (22), and the limiting threaded rod (28) above the top plate (27) is provided with a nut.