CN111713621A - Selenium-rich quinoa fermented milk and preparation method thereof - Google Patents

Selenium-rich quinoa fermented milk and preparation method thereof Download PDF

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CN111713621A
CN111713621A CN202010034925.3A CN202010034925A CN111713621A CN 111713621 A CN111713621 A CN 111713621A CN 202010034925 A CN202010034925 A CN 202010034925A CN 111713621 A CN111713621 A CN 111713621A
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quinoa
milk
selenium
malt
fermented milk
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韩卓
李慧
马龙
李亮
李妍
曹珂珂
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Bengbu College
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus

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  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses selenium-rich quinoa fermented milk and a preparation method thereof, wherein quinoa is screened, selected, soaked and germinated to prepare quinoa malt; collecting buds of rhizoma et radix Veratri, cleaning, air drying, and pulverizing to obtain rhizoma et radix Veratri bud powder; mixing quinoa malt powder with deionized water, and grinding to obtain quinoa malt milk; quinoa malt milk is inoculated with lactobacillus bulgaricus and streptococcus thermophilus after gelatinization, selenium addition, sterilization, cooling and strain activation, and then fermented to prepare selenium-rich quinoa fermented milk; the protein content of the quinoa fermented milk is more than or equal to 3.8 percent; the fat content is more than or equal to 3.4 percent; the solid of the non-fat milk is more than or equal to 9.2 percent; acidity 75-85 ° T; the viable count is 9.5-9.8log CFU/mL. Compared with the traditional quinoa fermented milk, the contents of protein, fat, non-fat milk solid and total flavonoids in the fermented milk are obviously improved, the acidity is slightly reduced, and the influence of overhigh acidity of the fermented milk on the taste is avoided; the trace selenium is added into the fermented milk, so that the health care function of the fermented milk is enhanced while the original flavor is maintained and the nutrient content is improved, and the health of people lacking selenium is improved.

Description

Selenium-rich quinoa fermented milk and preparation method thereof
Technical Field
The invention relates to the technical field of grain lactobacillus beverages, and particularly relates to selenium-rich quinoa fermented milk and a preparation method thereof.
Background
With the rapid development of economy in China, the living standard of people is increasingly improved, the consumption of grain lactobacillus products is continuously increased, and a grain lactobacillus beverage integrating organic, natural, healthy and nutritional functions is more popular with the masses. Due to the unique comprehensive nutritive value of the quinoa, the quinoa is considered as the only single plant food which can meet the basic nutritional requirements of human bodies by combining the food and agricultural organization of China.
Selenium and vitamin E are antioxidants, and supplement each other, so that the selenium and vitamin E can prevent aging and tissue hardening caused by oxidation, and can reduce the change speed at least; it also has effects of activating immune system and preventing cancer, and is essential trace mineral. Selenium is involved in the formation of many enzymes, particularly glutathione peroxidase, which protect cells and tissues and maintain their normal function. Selenium also has a detoxifying effect because selenium has a good affinity for metals. Selenium has strong oxidation resistance, and has cardiovascular protection and antitumor effects. Regulation of thyroid hormone; maintain normal immunity, anti-AIDS effect, and maintain normal fertility.
However, the traditional quinoa fermented milk has a single health-care function, is lack of selenium-enriched fermented milk, and has low contents of fat, protein, non-fat milk solid and total flavonoids and high acidity.
Disclosure of Invention
The invention aims to provide selenium-rich quinoa fermented milk and a preparation method thereof, and aims to solve the problems that the conventional quinoa fermented milk is single in health care function, is lack of selenium-rich fermented milk, and is low in fat, protein, non-fat milk solid and total flavone content and high in acidity.
In order to achieve the purpose, the invention provides the following technical scheme: selenium-rich quinoa fermented milk and a preparation method thereof, and is characterized in that: sieving quinoa, selecting, soaking, and germinating to obtain quinoa malt; collecting buds of rhizoma et radix Veratri, cleaning, air drying, and pulverizing to obtain rhizoma et radix Veratri bud powder; mixing quinoa malt powder with deionized water, and grinding to obtain quinoa malt milk; quinoa malt milk is inoculated with lactobacillus bulgaricus and streptococcus thermophilus after gelatinization, selenium addition, sterilization, cooling and strain activation, and then fermented to prepare selenium-rich quinoa fermented milk; the protein content of the quinoa fermented milk is more than or equal to 3.8 percent; the fat content is more than or equal to 3.4 percent; the solid of the non-fat milk is more than or equal to 9.2 percent; acidity 75-85 ° T; the viable count is 9.5-9.8log CFU/mL; the total flavone content is 36-39mg/100 mL.
The preparation method of the quinoa fermented milk comprises the following steps:
1) quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 1-3: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200-300 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: mixing skimmed milk powder with deionized water at a ratio of 1: 10, dissolving thoroughly, sterilizing at 115 deg.C under high temperature and high pressure for 15min to obtain culture medium, inoculating 0.5g mixed strain of Lactobacillus bulgaricus and Streptococcus thermophilus in biological clean safe cabinet into the culture medium, and shaking in gas bath constant temperature culture oscillator for two hours for activating strain. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the leaven into the cooled quinoa malt milk according to the inoculation amount of 3.5-4.5%.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant-temperature constant-humidity incubator at a certain temperature for a certain time.
Preferably, the ratio of lactobacillus bulgaricus to streptococcus thermophilus is 1: 2.2-3.7.
Preferably, the fermentation temperature of the quinoa malt milk is 38-43 ℃, and the fermentation time is as follows: 6-8 h.
Preferably, the lactobacillus bulgaricus is lactobacillus delbrueckii subsp bulgaricus.
Compared with the prior art, the invention has the beneficial effects that: through deep research on the fermentation temperature, strains and addition proportion, the quinoa fermented milk beverage with milk yellow color and delicious taste is screened out, the contents of protein, fat, non-fat milk solid and total flavone in fermented milk are obviously improved compared with the traditional quinoa fermented milk, and the acidity is slightly reduced, so that the influence of overhigh acidity of the fermented milk on the pure taste is avoided; in addition, the trace selenium is added into the fermented milk, so that the health-care function of the fermented milk is enhanced while the original flavor is maintained and the nutritional ingredients are improved, the health of people lacking selenium is improved, and the fermented milk has a wide market prospect.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The test was conducted in the microbiology laboratory at Union mussel-Port school on days 5 and 5 to 30 in 2019.
The sources of strains and materials are as follows:
Figure BSA0000200321250000041
example 1
1) Quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 1: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: the skim milk powder and deionized water are mixed fully at a ratio of 1: 10, dissolved and sterilized at high temperature and high pressure of 115 ℃ for 15min to prepare a culture medium, 0.5g of mixed strains of lactobacillus bulgaricus and streptococcus thermophilus at a ratio of 1: 2.5 are inoculated into the culture medium in a biological clean safety cabinet, and the strains are activated by shaking in an air bath constant temperature culture oscillator for two hours. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the leaven into the cooled quinoa malt milk according to the inoculation amount of 3.5.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant temperature and humidity incubator at 38 ℃ for 6 h.
The quinoa fermented milk product prepared by the processing has a milky yellow color and a unique flavor; the detection shows that the protein content of the product is 3.9 percent, the fat content is 3.4 percent, the solid content of the non-fat milk is 9.5 percent, the acidity is 78 DEG T, and the viable count is 9.5 logCFU/mL; the total flavone content is 37mg/100 mL.
Example 2
1) Quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 1.5: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: the skim milk powder and deionized water are mixed fully at a ratio of 1: 10, dissolved and sterilized at high temperature and high pressure of 115 ℃ for 15min to prepare a culture medium, 0.5g of mixed strains of lactobacillus bulgaricus and streptococcus thermophilus at a ratio of 1: 2.7 are inoculated into the culture medium in a biological clean safety cabinet, and the strains are activated by shaking in an air bath constant temperature culture oscillator for two hours. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the leaven into the cooled quinoa malt milk according to the inoculation amount of 3.7.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant temperature and humidity incubator at 38 ℃ for 7 h.
The quinoa fermented milk product prepared by the processing has a milky yellow color and a unique flavor; the detection shows that the protein content of the product is 3.8 percent, the fat content is 3.6 percent, the solid content of the non-fat milk is 9.2 percent, the acidity is 82 DEG T, and the viable count is 9.7 logCFU/mL; the content of total flavone is 36mg/100 mL.
Example 3
1) Quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 2: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: the skim milk powder and deionized water are mixed and fully dissolved at the ratio of 1: 10, and the mixture is sterilized at the high temperature and the high pressure of 115 ℃ for 15min to prepare a culture medium, 0.5g of mixed strains of the lactobacillus bulgaricus and the streptococcus thermophilus at the ratio of 1: 2.9 are inoculated into the culture medium in a biological clean safe cabinet, and the strains are activated by shaking in an air bath constant temperature culture oscillator for two hours. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the leaven into the cooled quinoa malt milk according to the inoculation amount of 3.9.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant temperature and humidity incubator at 38 ℃ for 7 h.
The quinoa fermented milk product prepared by the processing has a milky yellow color and a unique flavor; the detection shows that the protein content of the product is 4.3 percent, the fat content is 3.7 percent, the solid content of the non-fat milk is 9.7 percent, the acidity is 79 DEG T, and the viable count is 9.6 logCFU/mL; the content of total flavone is 39mg/100 mL.
Example 4
1) Quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 1: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: the skim milk powder and deionized water are mixed fully at a ratio of 1: 10, dissolved and sterilized at high temperature and high pressure of 115 ℃ for 15min to prepare a culture medium, 0.5g of mixed strains of lactobacillus bulgaricus and streptococcus thermophilus at a ratio of 1: 3.1 are inoculated into the culture medium in a biological clean safety cabinet, and the strains are activated by shaking in an air bath constant temperature culture oscillator for two hours. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the leaven into the cooled quinoa malt milk according to the inoculation amount of 4.1.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant temperature and humidity incubator at 38 ℃ for 8 h.
The quinoa fermented milk product prepared by the processing has a milky yellow color and a unique flavor; the detection shows that the protein content of the product is 4.6 percent, the fat content is 3.5 percent, the solid content of the non-fat milk is 9.8 percent, the acidity is 80 DEG T, and the viable count is 9.8 logCFU/mL; the content of total flavone is 39mg/100 mL.
Example 5
1) Quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 2: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: the skim milk powder and deionized water are mixed fully at a ratio of 1: 10, dissolved and sterilized at high temperature and high pressure of 115 ℃ for 15min to prepare a culture medium, 0.5g of mixed strains of lactobacillus bulgaricus and streptococcus thermophilus at a ratio of 1: 3.3 are inoculated into the culture medium in a biological clean safety cabinet, and the strains are activated by shaking in an air bath constant temperature culture oscillator for two hours. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the starter to the cooled quinoa malt milk according to the inoculation amount of 4.3.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant temperature and humidity incubator at 38 ℃ for 8 h.
The quinoa fermented milk product prepared by the processing has a milky yellow color and a unique flavor; the detection shows that the protein content of the product is 3.98 percent, the fat content is 3.9 percent, the solid content of the non-fat milk is 9.7 percent, the acidity is 83 DEG T, and the viable count is 9.6 logCFU/mL; the content of total flavone is 38mg/100 mL.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. Selenium-rich quinoa fermented milk and a preparation method thereof, and is characterized in that: sieving quinoa, selecting, soaking, and germinating to obtain quinoa malt; collecting buds of rhizoma et radix Veratri, cleaning, air drying, and pulverizing to obtain rhizoma et radix Veratri bud powder; mixing quinoa malt powder with deionized water, and grinding to obtain quinoa malt milk; quinoa malt milk is inoculated with lactobacillus bulgaricus and streptococcus thermophilus after gelatinization, selenium addition, sterilization, cooling and strain activation, and then fermented to prepare selenium-rich quinoa fermented milk; the protein content of the quinoa fermented milk is more than or equal to 3.8 percent; the fat content is more than or equal to 3.4 percent; the solid of the non-fat milk is more than or equal to 9.2 percent; acidity 75-85 ° T; the viable count is 9.5-9.8log CFU/mL; the total flavone content is 36-39mg/100 mL.
2. The selenium-rich quinoa fermented milk and the preparation method thereof according to claim 1, wherein the selenium-rich quinoa fermented milk comprises the following steps:
1) quinoa seeds: fresh, filled seeds are selected.
2) Sieving and selecting: and (3) carefully selecting fresh and plump quinoa seeds to remove impurities.
3) Soaking: the method comprises the steps of firstly cleaning seeds with clear water, then putting the seeds into deionized water for soaking for 30 minutes, then cleaning the seeds with flowing deionized water for 10 minutes, and after cleaning, putting the seeds into the deionized water again for soaking for 3 hours.
4) And (3) germination and bud collection: adding a proper amount of deionized water at the bottom of the bean sprout growing machine, putting the cleaned quinoa into the bean sprout growing machine, connecting a power supply, setting the temperature to be 30 ℃, waiting for 24 hours, and collecting the quinoa malt.
5) Cleaning: rubbing and peeling the germinated quinoa malt, and washing with deionized water.
6) Air-blast drying and crushing: placing the cleaned quinoa sprouts into an electric heating constant-temperature blast drying oven, setting the temperature at 30 ℃, taking out the quinoa sprouts after 24 hours, crushing the quinoa sprouts by using a crusher to obtain 60-mesh quinoa sprout powder, and preserving the quinoa sprout powder at low temperature.
7) Grinding: mixing rhizoma et radix Veratri Tribuli bud powder with 60 deg.C deionized water at a ratio of 1-3: 6(g/mL), and grinding with a food processor for 2 min.
8) Pasting: placing the grinded quinoa malt milk in a constant temperature magnetic stirrer at 80 deg.C, heating and stirring for 60min until paste is formed.
9) Adding selenium: adding a proper amount of sodium selenite into the gelatinized quinoa malt milk, and adding 200-300 mu g of sodium selenite into each kilogram of quinoa malt milk.
10) And (3) sterilization and cooling: adding water to water line before use, placing gelatinized and selenium-added quinoa sprout milk in a sterilizer, closing cover, checking state of exhaust valve and safety valve, and turning on power supply. When the pressure rises to 0.15MPa (121 ℃), the sterilizing pot automatically deflates, and then the time is started for 15 min. After the specified time is reached, the power supply is closed, and the air release valve is opened to release air slowly; when the pressure indicator is reduced to 0.00MPa, the air release valve can be opened to take out the quinoa malt milk when no steam is discharged, and the quinoa malt milk is cooled.
11) Activating strains: mixing skimmed milk powder with deionized water at a ratio of 1: 10, dissolving thoroughly, sterilizing at 115 deg.C under high temperature and high pressure for 15min to obtain culture medium, inoculating 0.5g mixed strain of Lactobacillus bulgaricus and Streptococcus thermophilus in biological clean safe cabinet into the culture medium, and shaking in gas bath constant temperature culture oscillator for two hours for activating strain. Making into starter, and storing at 0-4 deg.C.
12) Inoculation: inoculating the leaven into the cooled quinoa malt milk according to the inoculation amount of 3.5-4.5%.
13) And (3) heat preservation fermentation: fermenting and culturing the quinoa malt milk in a constant-temperature constant-humidity incubator at a certain temperature for a certain time.
3. The selenium-rich quinoa fermented milk and the preparation method thereof according to claim 2, wherein the selenium-rich quinoa fermented milk is prepared by the following steps: the ratio of lactobacillus bulgaricus to streptococcus thermophilus is 1: 2.2-3.7.
4. The selenium-rich quinoa fermented milk and the preparation method thereof according to claim 2, wherein the selenium-rich quinoa fermented milk is prepared by the following steps: the fermentation temperature of the quinoa malt milk is 38-43 ℃; fermentation time: 6-8 h.
5. The selenium-rich quinoa fermented milk and the preparation method thereof according to claim 1, wherein the selenium-rich quinoa fermented milk is prepared by the following steps: the lactobacillus bulgaricus is lactobacillus delbrueckii subsp bulgaricus.
CN202010034925.3A 2020-01-08 2020-01-08 Selenium-rich quinoa fermented milk and preparation method thereof Pending CN111713621A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114568687A (en) * 2022-03-10 2022-06-03 上海交通大学 Preparation method of quinoa compound whole grain enzyme

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106418089A (en) * 2016-12-19 2017-02-22 山西大学 Chenopodium quinoa fermented milk and preparation method thereof
CN107494731A (en) * 2017-09-27 2017-12-22 光明乳业股份有限公司 A kind of selenium-enriched probioticses acidified milk and preparation method thereof
CN109393281A (en) * 2018-12-13 2019-03-01 赵雪玲 The preparation method of black fungus quinoa composite fermented beverage

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106418089A (en) * 2016-12-19 2017-02-22 山西大学 Chenopodium quinoa fermented milk and preparation method thereof
CN107494731A (en) * 2017-09-27 2017-12-22 光明乳业股份有限公司 A kind of selenium-enriched probioticses acidified milk and preparation method thereof
CN109393281A (en) * 2018-12-13 2019-03-01 赵雪玲 The preparation method of black fungus quinoa composite fermented beverage

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114568687A (en) * 2022-03-10 2022-06-03 上海交通大学 Preparation method of quinoa compound whole grain enzyme

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