CN111713456A - Insect selectivity test device and method - Google Patents
Insect selectivity test device and method Download PDFInfo
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- CN111713456A CN111713456A CN202010686998.0A CN202010686998A CN111713456A CN 111713456 A CN111713456 A CN 111713456A CN 202010686998 A CN202010686998 A CN 202010686998A CN 111713456 A CN111713456 A CN 111713456A
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- 241000238631 Hexapoda Species 0.000 title claims abstract description 125
- 238000012360 testing method Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000017448 oviposition Effects 0.000 claims description 4
- 238000010998 test method Methods 0.000 claims description 4
- 230000037406 food intake Effects 0.000 claims description 3
- 235000012631 food intake Nutrition 0.000 claims description 3
- 244000309464 bull Species 0.000 abstract description 3
- 230000000903 blocking effect Effects 0.000 description 15
- 235000013399 edible fruits Nutrition 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 230000005484 gravity Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241001414826 Lygus Species 0.000 description 1
- 241001414823 Lygus hesperus Species 0.000 description 1
- 235000009470 Theobroma cacao Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 244000240602 cacao Species 0.000 description 1
- 230000009193 crawling Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses an insect selectivity test device and method, wherein the insect selectivity test device comprises: the cylindrical container, the top surface hangs establishes the columniform in the container, and with the coaxial worm releasing box that sets up of container, but the box body bottom of worm releasing box is equipped with open closed bottom plate, the center along the inside bottom surface of container extends to the radius direction and sets up the radius line, the central angle between all adjacent radius lines equals, set up at least one ring that uses the inside bottom surface center of container as the centre of a circle on the inside bottom surface of container, all rings outwards equidistance along the bottom surface center of container distributes, all fan-shaped regions that all rings and all radius lines enclose are used for placing different sources and the unanimous smell source of size. The problem that the throwing of flying insect is difficult, bull flying insect can not carry out the selectivity simultaneously experimental is solved to this application to and spatial structure tissue places as the smell source difficult, a plurality of smell sources can not test simultaneously.
Description
Technical Field
The invention relates to the technical field of insect ethology, in particular to an insect selectivity test device and method.
Background
In the technical field of insect behavior, the selectivity of insects to different host plants or varieties is often required to be researched in an indoor environment so as to determine the influence of different plant odor sources on insect behaviors and further provide data support for an insect behavior selection mechanism. The existing insect selective device mainly comprises a wind tunnel, a Y-shaped olfactometer and a four-arm olfactometer, but the requirements of the existing insect selective device on the number of odor sources are fixed, the existing insect selective device is respectively used for the selectivity of insects on 1, 2 and 4 odor sources, the selectivity of the insects on various odor sources at the same time cannot be met, and in addition, the existing insect selective device can only measure one insect at a time. Another assay, the leaf-saucer method, is mainly used for the selectivity of the crawling larvae or adults on different leaves, and has limitations: the method is carried out in a culture dish, and only plane leaves are suitable as the odor source, but tissues (such as fruits, potted seedlings and the like) with three-dimensional structures are not suitable as the odor source; the insect throwing position is manually controlled, and the insect throwing position cannot be ensured to be positioned in the center of the leaf disc every time; flying insects cannot be thrown.
For flying insects, the existing wind tunnel device can perform selective measurement of a single insect on a single odor source, but cannot realize the simultaneous measurement of a plurality of insects on a plurality of odor sources.
In summary, how to measure a plurality of odor sources by a plurality of insects at the same time is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the present invention provides an insect selectivity test apparatus and method, which can solve the problems that flying insects are difficult to throw, multiple flying insects cannot perform selectivity test simultaneously, and a three-dimensional structure is difficult to place as an odor source, and multiple odor sources cannot be tested simultaneously.
In order to achieve the above purpose, the invention provides the following technical scheme:
an insect selectivity test device comprising: the container comprises a cylindrical container, wherein the container is provided with an air vent, a cylindrical insect releasing box which is coaxially arranged with the container is hung on the inner top surface of the container and comprises a box body and a bottom plate which is arranged at the bottom of the box body in an openable and closable manner, the inner bottom surface of the container is provided with a radius line which extends along the center of the inner bottom surface of the container in the radius direction, all adjacent radius lines have the same central angle, the inner bottom surface of the container is provided with at least one circular ring which takes the center of the inner bottom surface of the container as the center of the circle, all the circular rings are distributed outwards at equal intervals along the center of the bottom surface of the container, and all the circular rings and all fan-shaped areas which are surrounded by the radius lines are used for placing odor sources with different sources and have the same size.
Preferably, the container comprises a cage body and a gauze wrapped on the periphery of the cage body, and an opening capable of being opened and closed is arranged on the gauze.
Preferably, an upper hanging piece is arranged at the center of the top surface of the interior of the container, and a lower hanging piece matched with the upper hanging piece is arranged at the center of the top surface of the exterior of the insect releasing box.
Preferably, the upper hanging piece and the lower hanging piece are both magnetic pieces, and the lower hanging piece is attracted to the upper hanging piece through magnetic attraction.
Preferably, the bottom of the box body is provided with a detachable bottom cover, and the bottom plate is arranged on the bottom cover in an openable and closable manner.
Preferably, the bottom includes annular lid edge, the internal week on lid edge be equipped with the lid is along radial unanimous pivot, the bottom plate includes two semicircular folded sheets, two the straight flange of folded sheet connect respectively in the both sides of pivot, and two when the folded sheet level set up, two the folded sheet blocks the bottom opening on lid edge, the bottom mobilizable being equipped with of pivot is used for opening and closing two the ware that opens and shuts of folded sheet.
Preferably, the shutter comprises a stop lever rotatably arranged at the bottom of the rotating shaft, and the diameter of the stop lever is smaller than or equal to that of the rotating shaft.
Preferably, the bottoms of the two folding plates are respectively provided with a buckle, and when the blocking rod is rotated into the buckles, the blocking rod horizontally supports the two folding plates so that the two folding plates are positioned in the same horizontal plane.
Preferably, the two folding plates are mounted on the rotating shaft through folding buckles.
An insect selectivity test method applied to the insect selectivity test device according to any one of the above, comprising:
the method comprises the following steps that radial lines are arranged in a radial direction in an extending mode along the center of the inner bottom surface of a cylindrical container, so that central angles between all adjacent radial lines are equal, at least one circular ring taking the center of the inner bottom surface of the container as a circle center is arranged on the inner bottom surface of the container, and all the circular rings are distributed outwards at equal intervals along the center of the bottom surface of the container;
placing odor sources with different sources and consistent sizes in all fan-shaped areas defined by all the circular rings and all the radius lines;
a cylindrical insect releasing box which is coaxial with the container and is provided with an openable bottom plate is arranged in the container, and a preset number of insects are placed in the insect releasing box;
and opening the bottom plate, releasing the insects in the insect releasing box, and counting the food intake or egg laying amount of all the insects after preset time.
The problem that the throwing of flying insect is difficult, bull flying insect can not carry out the selectivity simultaneously experimental is solved to this application to and spatial structure tissue places as the smell source difficult, a plurality of smell sources can not test simultaneously.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic view of an insect selectivity test apparatus provided by the present invention;
FIG. 2 is a schematic view of a bottom cover according to the present invention;
fig. 3 is a top view of the bottom cover provided by the present invention.
In FIGS. 1-3:
1-upper hanger, 2-lower hanger, 3-folding plate, 4-rotating shaft, 5-upright post, 6-gauze, 7-insect releasing box, 8-sector area, 9-cover edge, 10-folding buckle, 11-buckle and 12-blocking rod.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The core of the invention is to provide an insect selectivity test device and method, which can solve the problems that flying insects are difficult to throw in, multiple flying insects cannot simultaneously carry out selectivity tests, and a three-dimensional structure tissue is difficult to place as an odor source, and a plurality of odor sources cannot be simultaneously tested.
Referring to fig. 1 to 3, fig. 1 is a schematic view of an insect selectivity test device provided in the present invention; FIG. 2 is a schematic view of a bottom cover according to the present invention; fig. 3 is a top view of the bottom cover provided by the present invention.
An insect selectivity test device comprising: the columniform container, the container is equipped with the air vent, the top surface hangs establishes columniform in the container, and with the coaxial worm releasing box 7 that sets up of container, worm releasing box 7 includes box body and open closed bottom plate that sets up in the box body bottom, be equipped with on the inside bottom surface of container along the inside bottom surface center of container to the radius direction extension set up the radius line, the central angle between all adjacent radius lines equals, set up at least one ring that uses the center of the inside bottom surface of container as the centre of a circle on the inside bottom surface of container, outside equidistance distribution of bottom surface center along the container of all rings, all fan-shaped region 8 that all rings and all radius lines enclose are used for placing different sources and the unanimous smell source of size.
It should be noted that the insect selectivity test device is of an inner sleeve structure, consists of an external container and an insect releasing box 7 which are both cylindrical structures, the insect releasing box 7 is suspended at the center of the top of the cage, the circle center distance and the circle angle scale lines at the bottom of the cage are convenient for the uniform arrangement of odor sources, the consistent distance of the odor sources annularly arranged from the insect releasing box 7 to the bottom of the cage is ensured, and the scientificity of the insect selectivity test is ensured.
The bottom of the container is provided with a circle which takes the axis of the container as the center of a circle, has equal scale lines at intervals of 1 degree of circumferential angle and has equal intervals from the center of the circle, and is used for placing and positioning the odor source sample. The odor sources need to be a plurality of odor sources with the same growth period and consistent size.
A plurality of odor sources with three-dimensional structures or planar structures are arranged at the bottom of the container, all fan-shaped areas 8 between two adjacent circular rings have the same center distance and circumference angle, a plurality of odor sources with even dozens of three-dimensional or planar structures can be simultaneously arranged, a plurality of test flying insects are arranged in the insect releasing box 7, the axis of the insect releasing box 7 is collinear with the axis of the container, dozens of insects can be simultaneously arranged in the insect releasing box 7, and thus, after the bottom cover of the insect releasing box 7 is opened, the selection opportunities of the insects for the odor sources are equal. The problem that the throwing of flying insect is difficult, bull flying insect can not carry out the selectivity simultaneously experimental is solved to this application to and spatial structure tissue places as the smell source difficult, a plurality of smell sources can not test simultaneously.
In actual use, according to the number of the odor sources, the circumferential angle interval degree between the positions where the odor sources are placed is calculated, and the calculation formula is as follows: the number of the interval degrees is 360 DEG/the number of the odor sources. After the calculation, the odor sources were placed, and taking 10 odor sources as an example, the odor sources were spaced apart by 36 °. 10 plant varieties and fruits (odor sources) which are in the same growth period and are consistent in size are collected, and according to the scale marks at the bottom of the container, the 10 odor sources are annularly placed at the positions which are in the same center distance and are spaced by 36 degrees in pairs to form a complete closed loop. After a certain amount of tested insects are subjected to starvation treatment for a proper time, the tested insects are placed in the insect release box 7, and the insect release box 7 is hung on the top of the cage. The whole insect selectivity test device is arranged in a dark environment, a red light lamp is hung right above the center outside a container, the influence of light on the insect selectivity is reduced, a bottom plate is opened to release insects, the insects can selectively search different odor sources to eat or lay eggs, biological data such as food taking amount or egg laying amount after a certain time are counted, so that once biological measurement is completed, and the biological measurement is generally repeated for three times.
To further illustrate the advantages of the present invention, the insect selectivity test apparatus was used to test the preference of lygus hesperus for 26 cocoa germplasm resources fruits. This example detects 60 insects and 26 treatment scent sources in a single test, i.e., one biological test; the wind tunnel device and the Y-type olfactometer can detect 1 odor treatment source and 1 insect at a time, and each odor treatment source needs to detect 60 insects, namely a biological test; the four-arm olfactometer can detect 3 treatment odor sources and 1 insect, and 60 insects need to be detected every 3 treatment odor sources, namely a biological test; the bladed disk method cannot be used in this embodiment. As can be seen from table 1, the number of insects needed in this example is 180, the time needed is 72h, and the number of operations is 3, which are all significantly less than other apparatuses and methods, on the premise of ensuring scientificity (three biological repeated experiments), which indicates that this example has very significant high efficiency. It should be noted that although 72 hours are needed, the feeding trace and the laying trace after 24 hours of treatment need to be manually investigated in each biological test process, and other devices and methods need to be manually operated and observed in the required time, so that the manual workload is reduced remarkably.
Table 1 comparison of efficiency of different selectivity test methods for detecting preference of lygus theacrinus for 26 germplasm resource fruits
Note: different letters after the same column of data show significant differences (P < 0.01). T: treating the odor source; CK: blank control odor source.
In addition to the above-described embodiments, the container preferably includes a cage and a screen 6 wrapped around the outer periphery of the cage, and the screen 6 is provided with an opening that can be opened and closed.
It should be noted that the cage body includes a plurality of upright columns 5, a sealed cage bottom and a sealed cage top, all upright columns 5 are arranged between the cage bottom and the cage top, all upright columns 5 are arranged along an outer ring of the cage top and an outer ring of the cage bottom, and all upright columns 5 are used for supporting the cage bottom and the cage top. The opening can be set up to annular opening, is equipped with annular zip fastener on annular opening, and the switching operation of annular zip fastener is equal to the switch cage body, adopts the opening and closing operation that the container that this embodiment provided can be convenient for the cage body to the container simple structure that this embodiment provided, the cost is lower.
In addition to the above embodiments, as a further preferable mode, the center of the inner top surface of the container is provided with an upper hanging piece 1, and the center of the outer top surface of the insect releasing box 7 is provided with a lower hanging piece 2 which is matched with the upper hanging piece 1 to be hung.
It should be noted that the upper hanger 1 and the lower hanger 2 can be matched with a hanger, the upper hanger 1 can be a hook or a hanging ring, when the upper hanger 1 adopts the hook, the lower hanger 2 can adopt the hanging ring, and when the upper hanger 1 adopts the hanging ring, the lower hanger 2 can adopt the hook. The insect releasing box 7 is hung in the hanging mode of the upper hanging piece 1 and the lower hanging piece 2, so that the insect releasing box 7 can be conveniently disassembled and assembled, the insect releasing box 7 can be stably placed in a container, and the test precision is improved.
In addition to the above embodiments, it is further preferable that the upper hanger 1 and the lower hanger 2 are both magnetic members, and the lower hanger 2 is magnetically attracted to the upper hanger 1.
It should be noted that the shape of the magnetic part may be cylindrical, the upper hanger 1 is a cylindrical magnetic part arranged on the top surface inside the cage body, the lower hanger 2 is a cylindrical magnetic part arranged on the top surface of the insect releasing box 7, the axis of the upper hanger 1 and the axis of the cage body are arranged on the same straight line, the axis of the lower hanger 2 and the axis of the insect releasing box 7 are arranged on the same straight line, when the upper hanger 1 and the lower hanger 2 are adsorbed, the position of the lower hanger 2 is adjusted to ensure that the axes of the upper hanger 1 and the lower hanger 2 are collinear, so as to ensure that the axes of the cage body and the insect releasing box 7 are collinear, and to improve the test precision.
In addition to the above embodiments, it is further preferable that a detachable bottom cover is provided at the bottom of the case, and the bottom plate is provided on the bottom cover so as to be openable and closable.
It should be noted that the bottom cover is detachably arranged at the bottom of the box body, when the bottom plate is damaged, the whole insect releasing box 7 does not need to be replaced by replacing the bottom cover, and the cost is reduced.
On the basis of the above embodiment, as a further preferred, the bottom cover includes annular cover edge 9, the cover edge 9 is provided with a rotating shaft 4 radially consistent with the cover edge 9 along the inner circumference, the bottom plate includes two semicircular folding plates 3, the straight edges of the two folding plates 3 are respectively connected to two sides of the rotating shaft 4, and when the two folding plates 3 are horizontally arranged, the two folding plates 3 block the bottom opening of the cover edge 9, and the bottom of the rotating shaft 4 is movably provided with an opener for opening and closing the two folding plates 3.
It should be noted that the bottom cover is connected with the bottom opening of the box body through the cover edge 9, the connection mode can adopt threaded connection or friction clamping, preferably adopts friction clamping, and is convenient to detach; the axial of pivot 4 and one of them diameter collineation along 9 of lid, two semicircular folded sheet 3 set up respectively in the both sides of pivot 4, two folded sheet 3's size is unanimous, in order to guarantee the unanimity of opening size, two folded sheet 3 can revolute 4 rotations of pivot, when the ware that opens and shuts is opened, two folded sheet 3 can be vertical downwards under the action of gravity, thereby open the bottom opening of box body, release insect, when the ware that opens and shuts is closed, the ware that opens and shuts supports two folded sheet 3 levels, in order to close the bottom opening of box body. The embodiment has simple structure and is convenient to process and operate.
In addition to the above embodiments, it is further preferable that the opening and closing device includes a stopper rod 12 rotatably disposed at the bottom of the rotating shaft 4, and the diameter of the stopper rod 12 is smaller than or equal to the diameter of the rotating shaft 4.
It should be noted that the blocking rod 12 and the rotating shaft 4 are disposed at the bottom of the rotating shaft 4 at a certain angle, the rotating shaft 4 is parallel to the rotating plane of the blocking rod 12, so that the blocking rod 12 does not contact the rotating shaft 4 when rotating, and the diameter of the blocking rod 12 is smaller than or equal to the diameter of the rotating shaft 4, so as to ensure that the two folding plates 3 are in a vertical state under the action of gravity when being opened, and are not blocked by the blocking rod 12.
In addition to the above embodiments, it is further preferable that the bottom portions of the two folding plates 3 are respectively provided with a buckle 11, and when the blocking lever 12 is rotated into the buckle 11, the blocking lever 12 horizontally supports the two folding plates 3 so that the two folding plates 3 are in the same horizontal plane.
It should be noted that, the buckle 11 is used for being connected with the blocking rod 12 in a clamping manner, the buckle 11 and the blocking rod 12 are detachably connected, when the blocking rod 12 is rotated into the buckle 11, the blocking state of the two folding plates 3 is at this time, the two folding plates 3 are in a horizontal state, and the opening at the bottom of the box body is blocked, and when the blocking rod 12 is rotated out of the two buckles 11 by rotating the blocking rod 12, the two folding plates 3 are in an open state, and the two folding plates 3 are vertically downward to open the opening at the bottom of the box body. The structure that this embodiment set up is simple, be convenient for processing and operation.
In addition to the above-described embodiments, it is further preferable that the two folding plates 3 are attached to the rotating shaft 4 by the folding buckle 10. One side of the folding buckle 10 is connected with the rotating shaft 4, and the other side is connected with the folding plate 3, so that the two folding plates 3 can rotate around the folding buckle 10 connected with the folding plates respectively, and the folding plates 3 can be opened and closed. The structure can ensure that the two folding plates 3 can only move to the horizontal when moving upwards and can not move upwards continuously, at the moment, the baffle rod 12 is utilized to block the bottoms of the two folding plates 3, so that the two folding plates 3 can not move under the action of external force, the bottom opening of the box body can not be opened even if the insect releasing box 7 is overturned, and the safety is improved.
An insect selectivity test method applied to the insect selectivity test device disclosed in any one of the above embodiments, comprising:
the bottom surface inside the container is provided with at least one circular ring taking the center of the bottom surface inside the container as the center of a circle, and all the circular rings are distributed outwards at equal intervals along the center of the bottom surface of the container;
placing odor sources with different sources and consistent sizes in all fan-shaped areas 8 defined by all circular rings and all radius lines;
a cylindrical insect releasing box 7 which is coaxial with the container and can be opened and closed on a bottom plate is arranged in the container, and a preset number of insects are placed in the insect releasing box 7;
and opening the bottom plate, releasing the insects in the insect releasing box 7, and counting the food intake or egg laying amount of all the insects after the preset time.
A plurality of odor sources with three-dimensional structures or planar structures are arranged at the bottom of a container, the odor sources with the same radius have the same center distance and circumference angle, a plurality of or even dozens of odor sources with the three-dimensional or planar structures can be simultaneously arranged, a plurality of flying insects to be tested are arranged in an insect releasing box 7, the axis of the insect releasing box 7 is collinear with the axis of the container, dozens of insects can be simultaneously arranged in the insect releasing box 7, and thus, after the bottom cover of the insect releasing box 7 is opened, the selection opportunities of the insects for the odor sources are equal, the problems that the flying insects are difficult to throw in and the odor sources with the three-dimensional structures are difficult to place in the existing leaf disc method are solved, the problem that the existing device cannot simultaneously test a plurality of odor sources is solved, and the selective test can be simultaneously carried out on the flying insects with a plurality of heads.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The insect selectivity test device and method provided by the invention are described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (10)
1. An insect selectivity test device, comprising: the container comprises a cylindrical container, wherein the container is provided with vent holes, a cylindrical insect releasing box (7) which is coaxially arranged with the container is hung on the inner top surface of the container, the insect releasing box (7) comprises a box body and a bottom plate which is arranged at the bottom of the box body in an openable and closable manner, radius lines are arranged on the inner bottom surface of the container in a manner of extending along the center of the inner bottom surface of the container to the radius direction, the central angles of all adjacent radius lines are equal, at least one circular ring which takes the center of the inner bottom surface of the container as the center of the circle is arranged on the inner bottom surface of the container, all the circular rings are distributed outwards at equal intervals along the center of the bottom surface of the container, and all fan-shaped areas (8) which are surrounded by the circular rings and all the radius lines are used for placing odor sources with.
2. The insect selectivity test device according to claim 1, wherein the container comprises a cage body and a gauze (6) wrapping the periphery of the cage body, and the gauze (6) is provided with an opening and a closing opening.
3. The insect selectivity test device according to claim 1, characterized in that the center of the inner top surface of the container is provided with an upper hanging piece (1), and the center of the outer top surface of the insect releasing box (7) is provided with a lower hanging piece (2) which is matched with the upper hanging piece (1) in hanging.
4. The insect selectivity test device according to claim 3, wherein the upper hanger (1) and the lower hanger (2) are both magnetic pieces, and the lower hanger (2) is attracted to the upper hanger (1) through magnetic attraction.
5. The insect selectivity test device of any one of claims 1 to 4, wherein the bottom of the box body is provided with a detachable bottom cover, and the bottom plate is arranged on the bottom cover in an openable and closable manner.
6. The insect selectivity test device according to claim 5, wherein the bottom cover comprises an annular cover edge (9), the inner periphery of the cover edge (9) is provided with a rotating shaft (4) which is radially consistent with the cover edge (9), the bottom plate comprises two semicircular folding plates (3), the straight edges of the two folding plates (3) are respectively connected to two sides of the rotating shaft (4), when the two folding plates (3) are horizontally arranged, the two folding plates (3) block the bottom opening of the cover edge (9), and the bottom of the rotating shaft (4) is movably provided with a shutter for opening and closing the two folding plates (3).
7. Insect selectivity test device according to claim 6, characterized in that the shutter comprises a stop lever (12) rotatably arranged at the bottom of the rotating shaft (4), the diameter of the stop lever (12) is smaller than or equal to the diameter of the rotating shaft (4).
8. The insect selectivity test device according to claim 7, characterized in that the bottoms of the two folding plates (3) are respectively provided with a buckle (11), and when the stop lever (12) is rotated into the buckle (11), the stop lever (12) horizontally supports the two folding plates (3) so that the two folding plates (3) are in the same horizontal plane.
9. Insect selectivity test device according to claim 6, characterized in that two of the folding plates (3) are mounted on the spindle (4) by means of folding buckles (10).
10. An insect selectivity test method applied to the insect selectivity test apparatus according to any one of claims 1 to 9, comprising:
the method comprises the following steps that radial lines are arranged in a radial direction in an extending mode along the center of the inner bottom surface of a cylindrical container, so that central angles between all adjacent radial lines are equal, at least one circular ring taking the center of the inner bottom surface of the container as a circle center is arranged on the inner bottom surface of the container, and all the circular rings are distributed outwards at equal intervals along the center of the bottom surface of the container;
placing odor sources with different sources and consistent sizes in all fan-shaped areas (8) formed by the circular rings and all radius lines;
a cylindrical insect releasing box (7) which is coaxial with the container and can be opened and closed on a bottom plate is arranged in the container, and a preset number of insects are placed in the insect releasing box (7);
and opening the bottom plate, releasing the insects in the insect releasing box (7), and counting the food intake or egg laying amount of all the insects after preset time.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142146A (en) * | 2021-05-14 | 2021-07-23 | 云南大学 | Device and method for establishing insect social barrier model |
| CN114946774A (en) * | 2022-06-17 | 2022-08-30 | 河南工业大学 | Testing arrangement is compared to many food choices of bin worm of crawling preference |
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| KR101258655B1 (en) * | 2012-04-05 | 2013-05-13 | 대한민국 | Atttraction and sensing mosquito tarp |
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| CN113142146A (en) * | 2021-05-14 | 2021-07-23 | 云南大学 | Device and method for establishing insect social barrier model |
| CN113142146B (en) * | 2021-05-14 | 2023-08-18 | 云南大学 | Device and method for establishing insect social obstacle model |
| CN114946774A (en) * | 2022-06-17 | 2022-08-30 | 河南工业大学 | Testing arrangement is compared to many food choices of bin worm of crawling preference |
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| CN111713456B (en) | 2022-02-18 |
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