CN111713204B - Application of effector protein expressed by effector factor RxLR129113 in promoting plant growth - Google Patents
Application of effector protein expressed by effector factor RxLR129113 in promoting plant growth Download PDFInfo
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- CN111713204B CN111713204B CN202010428718.6A CN202010428718A CN111713204B CN 111713204 B CN111713204 B CN 111713204B CN 202010428718 A CN202010428718 A CN 202010428718A CN 111713204 B CN111713204 B CN 111713204B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Abstract
The invention relates to the technical field of plant cultivation, and particularly discloses application of an effector protein expressed by an effector factor RxLR129113 in promoting plant growth. According to the invention, through deep research on effector protein expressed by an effector factor RxLR129113, plant seeds are soaked in the solution containing the effector protein, so that the obvious positive effect on promoting the germination of the plant seeds and the growth of sprouts is discovered at a happy place, and the growth and development of plant root systems can be stimulated by adding the solution containing the effector protein into a plant seedling raising substrate, thereby promoting the growth and development of seedlings.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to application of effector protein expressed by an effector factor RxLR129113 in promoting plant growth.
Background
The long-term dependence on chemical fertilizers has also raised some problems in promoting plant growth and increasing yield. Such as: (1) the nitrogen and phosphorus contents in water are increased, so that the eutrophication of rivers, lakes and internal seas causes excessive propagation of algae, deterioration of water quality and mass death of fishes and other aquatic organisms; (2) the soil can be acidified by simply applying chemical fertilizers in an excessive way for a long time. The ammonium ion content of organic and inorganic complexes in the soil solution and on the soil micelle is increased, the soil structure is damaged, the soil is hardened, and the agricultural production cost and the crop yield are directly influenced; (3) toxic components in food, feed and drinking water are increased, and the nitrogen compound content in well water and river water in fertilizer areas is increased and even exceeds the drinking water standard; (4) the soil microflora is destroyed. The excessive application of chemical fertilizers, especially nitrogen fertilizers, has killing and inhibiting effects on microorganisms, a large number of microorganisms die after long-term application, soil microbial flora changes, a plurality of beneficial microorganisms change from dominant species to secondary species, and crops are easy to suffer from various diseases.
The root system is an important absorption, synthesis, fixation and support organ of the plant, plays an important role in the growth and development process of the crop, and the robust root system can provide sufficient nutrients and water for the growth of the plant, thereby being the basis of high yield of the plant. At present, root research is internationally taken as a basic research topic with great potential for further improving crop productivity.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a new application of an effector protein expressed by an effector factor RxLR129113 in promoting the growth and development of plants.
Another purpose of the invention is to provide a method for promoting the germination of plant seeds or the growth of buds and the growth of plant roots or the development of seedlings.
It should be noted that the effector factor RxLR129113 described in the present invention has been described in chinese patent application publication No. CN110878315A (publication No. 03/13/2020), and the nucleotide sequence and the amino acid sequence of the expressed effector protein are not described in detail in the present invention.
The technical scheme provided by the invention is as follows:
in a first aspect, the present invention provides the use of an effector protein expressed by the effector factor RxLR129113 for promoting plant growth.
The application is the application of any one of the following aspects:
(1) promoting the germination of plant seeds;
(2) promoting the growth of plant buds;
(3) promoting the growth of plant roots;
(4) promoting the growth of plant seedlings.
More specifically, plant seeds are soaked by using a solution containing the effector protein, so that the germination of the seeds and the growth of sprouts are promoted; or by applying the solution containing the effector protein to a plant seedling raising substrate, the growth of plant root systems and the development of seedlings are promoted.
Alternatively, the plant of the present invention is a dicotyledonous plant, preferably a plant of the order violales, more preferably a plant of the family Cucurbitaceae (Cucurbitaceae), and even more preferably a plant of the genus Cucumis (Cucumis).
Experiments show that the effector protein expressed by the effector factor RxLR129113 has obvious positive effects on promoting seed germination, sprout growth, root growth, seedling development and the like of cucumber (Cucumis sativus). Furthermore, it is also assumed that plants of the genus Cucumis, which have a close relationship with Cucumis sativus, such as Cucumis bisexualis, Cucumis hystrix Chakrusei, Cucumis melo (Cucumis melo L.), and varieties thereof, such as Cucumis melo (Cucumis melo L.var. chinensis Pangal) can be promoted in terms of growth and development by the above effector proteins.
Furthermore, the effector protein can construct a genetic engineering bacterium containing an effector factor RxLR129113 by a genetic recombination means, and the effector factor is induced and expressed to obtain the effector protein.
In a second aspect, the present invention provides a method of promoting germination or shoot growth of a plant seed by soaking the plant seed with a solution containing an effector protein expressed by the effector factor RxLR 129113.
And the method for promoting the growth of plant root systems or the development of seedlings is characterized in that effector protein expressed by an effector RxLR129113 is added into a seedling raising matrix, and the plant seedlings are placed in the matrix for cultivation, so that the growth of the root systems of the plant seedlings is promoted, and the development of the seedling is more robust.
The raw materials or reagents involved in the invention are all common commercial products, and the operations involved are all routine operations in the field unless otherwise specified.
The above-described preferred conditions may be combined with each other to obtain a specific embodiment, in accordance with common knowledge in the art.
The invention has the beneficial effects that:
according to the invention, through deep research on effector protein expressed by effector factor RxLR129113, plant seeds are soaked in the solution containing the effector protein, positive effects of the effector protein on promoting germination of the plant seeds and growth of sprouts are discovered with pleasure, and the solution containing the effector protein is added into a seedling raising substrate, so that growth of plant root systems can be stimulated, and further growth and development of seedlings are promoted.
The invention is based on the prior invention and carries out the expanded research on the function of an effector RxLR 129113. Not only broadens the application range of the effect factor, but also provides a new inspiration and solution for plant cultivation technology.
Drawings
FIG. 1 is a map of pET28a vector in example 1 of the present invention;
FIG. 2 shows the result of PCR amplification of the RxLR129113 gene in example 1 of the present invention; wherein, M: DNA Marker, 1-4: PCR amplification band of RxLR129113 gene;
FIG. 3 is an SDS-PAGE gel analysis of the test expression of RxLR129113 in example 1 of the present invention; wherein, M: protein marker; 1-2: IPTG induces RxLR129113 protein expression;
FIG. 4 shows the effect of RxLR129113 protein solution on the shoot growth of cucumber seeds in example 1.
FIG. 5 shows the effect of the growth medium added with RxLR129113 protein solution on the growth vigor of 2-leaf stage cucumber seedlings in example 1 of the present invention.
FIG. 6 shows the effect of the seedling substrate added with RxLR129113 protein solution on the growth of cucumber seedlings in 4-5 leaf stage in example 1 of the present invention.
Detailed Description
Preferred embodiments of the present invention will be described in detail with reference to the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
1. Phytophthora capsici (Phytophthora capsaici) effector molecule RxLR129113 gene clone sequencing
1.1 strains of Phytophthora capsici Strong pathogenic Phytophthora capsici SD33 was stored in the vegetable pest biology focus laboratory of Shandong university of agriculture.
1.2 RNA extraction and reverse transcription of cDNA from the strain of Phytophthora capsici SD33
The strong pathogenic strain SD33 stored in the laboratory was cultured using V8 plates in a constant temperature incubator at 28 ℃.
1.2.1 RNA extraction procedure as follows:
1) grinding a sample: grinding phytophthora capsici SD33 mycelia into powder by using a mortar precooled by liquid nitrogen;
2) homogenizing: taking 1g of mycelium ground powder, adding 10mL of Trizol, fully homogenizing for 2min by an electric vortex instrument, standing for 3-5min at room temperature to fully crack;
3) centrifuging at 12000rpm at 4 deg.C for 10min, sucking supernatant and removing precipitate;
4) adding chloroform into 200 mu L chloroform/mL Trizol, shaking and mixing uniformly (a vortex shaking instrument is not used), standing for 15min at 25 ℃, and centrifuging for 15min at the rotation speed of 13000rpm at 4 ℃;
5) absorbing the upper water phase into a new centrifugal tube, discarding the lower phenol phase without absorbing the middle interface, wherein the phenol phase is used for extracting protein;
6) adding 500 mu L of isopropanol into 1mL of Trizol, reversing, and standing at room temperature for 5-10 min;
7) centrifuging at 4 deg.C and 12000rpm for 10min, and collecting supernatant;
8) adding absolute Ethanol according to the proportion of 1mL of 5% Ethanol/mL Trizol, gently inverting, centrifuging at the temperature of 4 ℃ and the rotation speed of 8000rpm for 5min, and removing the supernatant as much as possible;
9) standing at room temperature, air drying or oven drying for 5-10min, removing ethanol, and preventing RNA sample from drying excessively or dissolving;
10) using 50 μ L H2Dissolving O, TE buffer or 0.5% SDS, treating the solvent with DEPC, and autoclaving at 121 deg.C for 30 min;
11) OD measurement using ultraviolet spectrophotometry600，A260/A280 1.8～2.0，A260/A2301.8-2.2, which indicates negligible contamination and success.
1.2.2 RNA reverse transcription to synthesize cDNA, the flow of the instruction is as follows:
1) RNA reverse transcription is carried out on the RNA, an RNase removing centrifugal tube is prepared into a PCR system, and the preparation system is shown in table 1.
TABLE 1 RT-PCR reaction System
Sucking, beating and mixing after mixing, centrifuging and placing on ice.
2) Incubating at 50 deg.C for 15min, incubating at 85 deg.C for 2min, and immediately subjecting the product to PCR reaction, or storing at-20 deg.C for half a year; the long-term preservation is recommended to be carried out after subpackaging and then storing in a freezer at the temperature of-80 ℃, and repeated freeze thawing of cDNA is avoided.
1.3RxLR129113 Gene PCR cloning primer design
A pair of specific primers is designed according to the whole genome https of phytophthora capsici// genome. jgi. doe. gov/portal/RxLR 129113 gene sequence (signal peptide removal sequence) and two enzyme cutting sites NcoI and XhoI of a recombinant vector pET28 a:
upstream primer RxLR 129113F:
5’-CATGCCATGGTGGTGCCAGCAAAAGCCCAAT-3’；
downstream primer RxLR 129113R:
5’-CCGCTCGAGACCATTTCTCGCCGCCTTGT-3’。
for later purification of the RxLR129113 protein, the C-terminal his tag of pET28a vector was ensured to be translated normally, and the stop codon of RxLR129113 was deleted. The specific primer pair is synthesized by Qingdao organism in limited way.
The map of the pET28a vector is shown in FIG. 1.
1.4PCR amplification of the target Gene RxLR129113
The target fragment was amplified from the cDNA by polymerase chain reaction, the PCR reaction system is shown in Table 2.
TABLE 2RxLR129113 Gene PCR amplification System
The PCR amplification product is shown by agarose gel electrophoresis, and the amplification band is about 1200bp and is basically consistent with the size of the target gene band. The PCR amplified target gene fragment is recovered by using a gel recovery kit (DNA recovery kit), and the recovered product can be stored for later use at the temperature of minus 20 ℃ for a short time. The PCR amplification result of the target RxLR129113 gene is shown in FIG. 2.
2. Recombinant vector construction
2.1 double digestion of target Gene and vector
The RxLR129113 gene PCR amplification gel recovered product and the vector pET28a are subjected to double enzyme digestion by using enzyme 1(NcoI) and enzyme 2(XhoI), respectively, and are subjected to water bath at 37 ℃ for 2-3 h, wherein the reaction system of the double enzyme digestion is shown in Table 3. The RxLR129113 and the vector pET-28a after the enzyme digestion are recovered by using a gel recovery kit.
TABLE 3 double digestion reaction System for RxLR129113 and vector pET28a
2.2 the target gene RxLR129113 is connected to the vector pET-28a
The digested RxLR129113 gene DNA fragment and the vector pET-28a are recovered and then connected by Solution I at 16 ℃ for 3h, and the connection system is shown in Table 4.
TABLE 4 ligation of RxLR129113 to vector pET-28a reaction System
2.3 transformation of the pET-28a recombinant vector into E.coli DH5 alpha competent
1) Melting 50 μ L DH5 α competent cells in ice bath, adding ligation product, mixing gently, and ice-cooling for 30 min;
2) carrying out water bath heat shock for 90s at 42 ℃, and then rapidly carrying out ice bath on the centrifugal tube for 2 min;
3) adding 500 mu L of sterile LB culture medium (without antibiotics) into each centrifuge tube on a super clean bench, uniformly mixing, placing at 37 ℃, performing shaking culture at 200rpm for 45-60 min, and ensuring that host bacteria are completely recovered;
4) centrifuging for 1min at 8000rpm of a normal temperature centrifuge, discarding part of supernatant, resuspending with a pipette thallus, uniformly mixing, coating on an LB agar culture medium (containing corresponding antibiotics), and then performing inverted culture in an incubator at 37 ℃ for 12-16 h.
2.4 identification of the recombinant vector pET28a
After bacterial plaque grows on the plate by the prepared competent cells, adding 1.2mL of sterile LB liquid medium (containing corresponding antibiotics) into a 2mL centrifuge tube, selecting the grown single bacterial colony, placing the single bacterial colony into the LB liquid medium centrifuge tube, carrying out shake culture at 37 ℃ for 5-6 h to serve as a bacterial liquid PCR template, and carrying out bacterial liquid PCR identification at proper time, wherein a bacterial liquid PCR reaction system is shown in Table 5.
TABLE 5 PCR reaction system for recombinant vector pET28a bacterial liquid
Carrying out agarose gel electrophoresis identification on a bacteria liquid PCR reaction sample, taking a positive sample with a PCR amplification result as the positive sample, taking 400 mu L of the bacteria liquid of the positive sample, sending the positive sample to Qingdao Zhike biology Limited company for sequencing, using DNAman software to compare and analyze a sequencing result with a genome sequence, taking 840 mu L of the bacteria liquid with a correct sequencing result, adding 160 mu L of 50% sterilized glycerol, storing the bacteria liquid in a freezer at the temperature of-20 ℃, taking another bacteria liquid with a correct sequencing result to extract plasmids, and storing the plasmids in the freezer at the temperature of-20 ℃ for later use, wherein the plasmid extraction is carried out according to the instruction of a CWBIO high-purity plasmid mini-extraction kit.
RxLR129113 recombinant protein expression
3.1 test expression of RxLR129113 recombinant protein
1) The pET28a recombinant plasmid with correct sequencing is transformed into an E.coli Rosetta (DE3) strain, centrifuged for 1min at 8000rpm of a normal-temperature centrifuge, a part of supernatant is discarded, a pipettor is used for carrying out heavy suspension, the mixture is uniformly mixed and then coated on an LB agar plate culture medium (containing corresponding antibiotics), and then the inverted culture is carried out for 12 to 16 hours in an incubator at 37 ℃;
2) selecting a single bacterial strain, inoculating the single bacterial strain into a centrifugal tube filled with 1.5mL of LB liquid medium (added with Kan resistance), and carrying out shake culture at 37 ℃ and 180rpm for 6 h;
3) inoculating 1mL of shaking culture solution into an LB liquid culture medium (added with Kan resistance) containing 1.5mL of the shaking culture solution, culturing until OD600 is 0.6-0.8, and taking 1mL of bacterial solution as a control before induction;
4) adding Inducer (IPTG) with different concentrations into the induced bacteria liquid and the control bacteria liquid respectively, and inducing at 37 ℃ and 180rpm for 3 h;
5) after induction, 1mL of bacterial liquid is taken respectively, and is centrifuged for 1min at 12000rpm simultaneously with the control; discarding the supernatant, respectively adding 40 μ L of 2 × Binding Buffer for resuspension and mixing, and then adding 40 μ L of 2 × Loading Buffer for mixing;
6) boiling the sample for 15min, oscillating for 1 time at 5min intervals, oscillating for 2 times, and centrifuging at 12000rpm for 1min before SDS-PAGE electrophoresis;
7) taking 20 mu L of sample to carry out SDS-PAGE electrophoresis, observing the expression condition of the target protein, and determining that the appropriate concentration of an Inducer (IPTG) is 0.5 mM;
8) 840 mu L of the expressed target protein bacterial liquid is taken, 160 mu L of 50 percent of sterilized glycerol is added, mixed evenly, filled into a sterilized freezing storage tube and stored in a freezer at the temperature of minus 20 ℃.
And then detecting the expression condition of the target protein by using SDS-PAGE electrophoresis technology, wherein SDS-PAGE electrophoresis results show that compared with a control, after the pET-28a-RxLR129113 protein is induced by a proper concentration Inducer (IPTG), the RxLR129113 is induced to express a protein with a molecular weight of 50KDa band, the molecular weight of the protein is consistent with the predicted molecular weight of the PcRxLR129113, and the construction of the pET-28a-RxLR129113 protein prokaryotic expression system is successful, and the result is shown in FIG. 3.
3.2 Mass expression of RxLR129113 recombinant protein
1) Activating the preserved strain with higher expression level, inoculating the strain into 1L LB liquid culture medium containing 50mg/mL Kan according to the proportion of 1:100, and performing shake culture at 37 ℃ and 180rpm for 3-4 h to ensure the OD of the strain600＝0.6～0.8；
2) And (3) ensuring that the temperature of the shaking table is reduced to 16 ℃ in advance, adding an Inducer (IPTG) with the final concentration of 0.5mM, inducing at the temperature of 16 ℃ overnight at 120rpm for 16-21 h to ensure that the OD value is (1.8-2.0), starting to induce for 16h, monitoring the OD value by using an ultraviolet spectrophotometer, and monitoring for 1 time every 1 h.
OD value: the optical density of the protein to be detected was expressed, and 1OD corresponds to E.coli concentration ≈ 108～109cell/mL.
Promoting germination and growth vigor of cucumber seeds and root systems of seedlings by using RxLR129113 protein
4.1RxLR129113 protein promotes germination and sprout growth of cucumber seeds
Soaking cucumber seeds in warm water at 55 ℃ for 5-8 min, diluting the RxLR129113 protein (OD value is 1.8-2.0) prepared by induction by 2-3 times with sterile water, soaking the cucumber seeds in the diluted protein solution for 5min, and simultaneously using LB liquid culture medium as control treatment (CK), and then carrying out germination accelerating treatment according to the conventional technology.
Comparison of the germination and sprout growth of cucumber seeds 2 days after treatment is shown in fig. 4, the average length of the sprouts of 30 cucumber seeds treated by the RxLR129113 protein is 2.3cm, while the average length of the sprouts of 30 cucumber seeds treated by the control is only 1.6cm, the average length of the sprouts of the former cucumber seeds is increased by 0.7cm compared with the average length of the latter cucumber seeds, and the RxLR129113 protein solution can obviously promote the sprout growth of cucumber seeds.
4.2RxLR129113 protein solution for promoting root growth of cucumber seedlings
Diluting 2-3 times of RxLR129113 protein solution with OD value of 1.8-2.0 by using sterile water, and before seedling culture, applying the protein diluted solution in a cucumber seedling culture substrate in a mixing manner to serve as a protein treatment group, wherein the using amount of the protein diluted solution is 100mL/100kg of the seedling culture substrate; LB liquid medium was added to the cucumber seedling substrate in the same ratio as a control group (CK).
And respectively and randomly planting cucumber sprouts with the same growth vigor in the cucumber seedling culture substrate added with the protein diluted solution and the LB liquid culture medium.
When the cucumber seedlings grow to the 2-leaf stage and the 4-5-leaf stage, the root growth conditions and the seedling states of the seedlings in different cucumber seedling raising substrates are respectively compared, and the results are shown in fig. 5 and 6.
FIG. 5 shows that the average length of the young root is 3.6cm (statistics of 15 seedling root systems) at 2-leaf stage of the cucumber seedling treated with protein, while the average length of the young root is 2.2cm (statistics of 15 seedling root systems) at 2-leaf stage of the cucumber seedling treated with control (LB medium), the former is increased by 1.4cm compared with the latter. And in fact, the protein-treated seedlings developed to the 2-leaf stage 8-10 days, while the control-treated seedlings developed to the 2-leaf stage 14 days.
FIG. 6 shows that the average length of the young root is 6.5cm (statistics of 15 seedling root systems) in the 4-5 leaf stage of the cucumber seedling treated with the protein, while the average length of the young root is 4.2cm (statistics of 15 seedling root systems) in the 4-5 leaf stage of the cucumber seedling treated with the control CK (LB medium), the former is increased by 2.3cm compared with the latter. And in fact, the seedlings of the protein-treated group developed to the 4-leaf stage on days 14-17, while the control-treated seedlings developed to the 4-leaf stage on days 21-23.
Therefore, the RxLR129113 protein can obviously promote the root growth and the seedling development of cucumber seedlings.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. Application of effector protein expressed by effector factor RxLR129113 in promoting cucumber (Cucumis) plant growth.
2. The application according to claim 1, wherein the application is any one of the following:
(1) promoting germination of cucumber plant seeds;
(2) promoting the growth of the young buds of the cucumber plants;
(3) promoting the root growth of the cucumber plants;
(4) promoting the development of cucumber plant seedlings.
3. Use according to claim 2, wherein the germination and shoot growth of cucumber seeds is promoted by soaking the cucumber seeds with a solution containing the effector protein;
or applying the solution containing the effector protein to a seedling substrate of the cucumber plant to promote root growth and seedling development of the cucumber plant.
4. Use according to claim 1, wherein the Cucumis plant is cucumber (Cucumis sativus).
5. The use of any one of claims 1 to 4, wherein the effector protein is induced to express by a genetically engineered bacterium comprising the effector factor RxLR 129113.
6. A method of promoting germination or shoot growth of cucumber seeds, comprising soaking the cucumber seeds with a solution comprising an effector protein expressed by an effector factor RxLR 129113.
7. A method for promoting root growth or seedling development of Cucumis plants is characterized in that effector protein expressed by effector factor RxLR129113 is added into a seedling substrate.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003048365A1 (en) * | 2001-12-04 | 2003-06-12 | Syngenta Mogen B.V. | Fungal elicitor |
| CN102612890A (en) * | 2012-04-09 | 2012-08-01 | 上海大学 | Method for improving vitality and cold resistance of corn seeds |
| CN103145816A (en) * | 2013-03-25 | 2013-06-12 | 中国农业科学院植物保护研究所 | Application of protein elicitor Hripl for improving and perfecting salt tolerance and drought resistance of plants |
| CN103210087A (en) * | 2010-09-06 | 2013-07-17 | 淡马锡生命科学研究院有限公司 | Molecular interaction between Xa10 and AvrXa10 |
| CN103820466A (en) * | 2014-01-20 | 2014-05-28 | 上海交通大学 | Plant gene HIR1 capable of being combined with Hpa1 and improving plant disease resistance and ageing resistance |
| WO2018101824A1 (en) * | 2016-11-30 | 2018-06-07 | Universiteit Van Amsterdam | Plants comprising pathogen effector constructs |
| CN108522508A (en) * | 2018-04-18 | 2018-09-14 | 中国农业科学院植物保护研究所 | Applications of the protein elicitor PeaT1 in inducing the anti-aphid of crops |
| CN108575998A (en) * | 2018-04-18 | 2018-09-28 | 中国农业科学院植物保护研究所 | Applications of the albumen exciton Hrip1 in inducing the anti-aphid of crops |
| CN109096378A (en) * | 2018-08-14 | 2018-12-28 | 黑龙江八农垦大学 | A kind of subtilisin exciton AMEP412 and its function |
| CN109134625A (en) * | 2018-08-14 | 2019-01-04 | 黑龙江八农垦大学 | A kind of general mycoprotein exciton Hcp of pineapple and its function |
| CN110483616A (en) * | 2019-07-31 | 2019-11-22 | 中国农业科学院农产品加工研究所 | The method of the apoplast effect protein of pathogen secretion is separated from the plant tissue of infection pathogen |
| CN110878315A (en) * | 2019-11-04 | 2020-03-13 | 山东农业大学 | Bacterial effector factor and coding gene and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003256401A1 (en) * | 2002-08-16 | 2004-03-03 | Boyce Thompson Institute For Plant Research | Backterial effector proteins which inhibit programmed cell death |
-
2020
- 2020-05-20 CN CN202010428718.6A patent/CN111713204B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003048365A1 (en) * | 2001-12-04 | 2003-06-12 | Syngenta Mogen B.V. | Fungal elicitor |
| CN103210087A (en) * | 2010-09-06 | 2013-07-17 | 淡马锡生命科学研究院有限公司 | Molecular interaction between Xa10 and AvrXa10 |
| CN102612890A (en) * | 2012-04-09 | 2012-08-01 | 上海大学 | Method for improving vitality and cold resistance of corn seeds |
| CN103145816A (en) * | 2013-03-25 | 2013-06-12 | 中国农业科学院植物保护研究所 | Application of protein elicitor Hripl for improving and perfecting salt tolerance and drought resistance of plants |
| CN103820466A (en) * | 2014-01-20 | 2014-05-28 | 上海交通大学 | Plant gene HIR1 capable of being combined with Hpa1 and improving plant disease resistance and ageing resistance |
| WO2018101824A1 (en) * | 2016-11-30 | 2018-06-07 | Universiteit Van Amsterdam | Plants comprising pathogen effector constructs |
| CN108522508A (en) * | 2018-04-18 | 2018-09-14 | 中国农业科学院植物保护研究所 | Applications of the protein elicitor PeaT1 in inducing the anti-aphid of crops |
| CN108575998A (en) * | 2018-04-18 | 2018-09-28 | 中国农业科学院植物保护研究所 | Applications of the albumen exciton Hrip1 in inducing the anti-aphid of crops |
| CN109096378A (en) * | 2018-08-14 | 2018-12-28 | 黑龙江八农垦大学 | A kind of subtilisin exciton AMEP412 and its function |
| CN109134625A (en) * | 2018-08-14 | 2019-01-04 | 黑龙江八农垦大学 | A kind of general mycoprotein exciton Hcp of pineapple and its function |
| CN110483616A (en) * | 2019-07-31 | 2019-11-22 | 中国农业科学院农产品加工研究所 | The method of the apoplast effect protein of pathogen secretion is separated from the plant tissue of infection pathogen |
| CN110878315A (en) * | 2019-11-04 | 2020-03-13 | 山东农业大学 | Bacterial effector factor and coding gene and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| Harpin Protein, an Elicitor of Disease Resistance, Acts;Hueywen Chuang;《J Plant Growth Regul》;20140513;第788-797页 * |
| SAUR Proteins as Effectors of Hormonal and;Hong Ren et al.;《Molecular Plant》;20180831(第8期);第1153-1164页 * |
| 交链孢菌蛋白激发子对棉子萌发及幼苗生长的影响;张志刚 等;《中国棉花》;20070515(第5期);第24-25页 * |
| 生物源蛋白激发子的研究进展;汪和贵 等;《广西植物》;20150615;第36卷(第4期);第413-418页 * |
| 真菌激活蛋白对农作物影响的研究进展;王成 等;《湖南农业科学》;20180827(第8期);第128-130页 * |
| 瞬时沉默乙醛脱氢酶对辣椒疫霉效应因子RxLR129113 功能的影响;王俪颖 等;《山东农业大学学报》;20190224;第50卷(第1期);第31-34页 * |
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